RLG00000008375

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003527
Physical Location & Seq
Reverse (-)
29755572 .. 29756011
440 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008375

Sequence Viewer

Length: 360 bp
ATGTTCGTCTTCTTTGGCCCCCTGAAGCCCGAACCACATATGATCAAACTCATCCACCATGCCACCGACACCGGCATCACCTTCCTTGATACCGCTGACCTTTATGGTCCCTTCACCAATGAAGTTCTTCTCGGCAAGCCTTTGAATGGAGGGGTGAGAGACAAGCTTGAATTGGCCACCAAATTCGATATCAGCTTTGAGCACAACAAGAGAGAGATTATTCGACGGGAGCCGGTACATGTGAGAGCTGCTAGTGAAGGAATCTTGAAATGCCTTCATGTAGACTCTATTGATCTCTATTTTCAGCATCGGATTGACACCCGTGTCCACATTCAAATCACGATCTATTTTCTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

120

Amino Acids

13.75

Weight (kDa)

6.7

Isoelectric Point (pI)

27.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 11 - 114 1.2e-15 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 323
AccI GTMKAC 1 cut(s) 282
AciI CCGC 1 cut(s) 93
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 1 cut(s) 182
AcuI CTGAAG 1 cut(s) 44
AfaI GTAC 1 cut(s) 237
AfiI CCNNNNNNNGG 1 cut(s) 146
AflIII ACRYGT 1 cut(s) 238
AgsI TTSAA 4 cut(s) 145, 170, 268, 335
AluBI AGCT 3 cut(s) 166, 195, 248
AluI AGCT 3 cut(s) 166, 195, 248
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 1 cut(s) 153
AoxI GGCC 2 cut(s) 16, 174
ApeKI GCWGC 1 cut(s) 248
ApoI RAATTY 1 cut(s) 182
Asp700I GAANNNNTTC 1 cut(s) 126
AspS9I GGNCC 2 cut(s) 17, 107
AsuHPI GGTGA 3 cut(s) 70, 106, 166
AvaII GGWCC 1 cut(s) 107
BalI TGGCCA 1 cut(s) 176
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 1 cut(s) 235
BclI TGATCA 1 cut(s) 42
BcoDI GTCTC 1 cut(s) 153
BfaI CTAG 1 cut(s) 252
BisI GCNGC 1 cut(s) 249
BlsI GCNGC 1 cut(s) 250
Bme18I GGWCC 1 cut(s) 107
BmgT120I GGNCC 2 cut(s) 17, 107
BmiI GGNNCC 3 cut(s) 19, 109, 231
BmsI GCATC 2 cut(s) 84, 316
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse118I RCCGGY 2 cut(s) 71, 232
BseGI GGATG 1 cut(s) 51
BseLI CCNNNNNNNGG 1 cut(s) 146
BseXI GCAGC 1 cut(s) 235
BshFI GGCC 2 cut(s) 18, 176
BsiHKAI GWGCWC 1 cut(s) 204
BsiSI CCGG 2 cut(s) 72, 233
BslFI GGGAC 1 cut(s) 93
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 1 cut(s) 153
BsmFI GGGAC 1 cut(s) 93
BsnI GGCC 2 cut(s) 18, 176
Bsp1286I GDGCHC 1 cut(s) 204
Bsp143I GATC 3 cut(s) 42, 292, 342
BspACI CCGC 1 cut(s) 93
BspANI GGCC 2 cut(s) 18, 176
BspLI GGNNCC 3 cut(s) 19, 109, 231
BsrFI RCCGGY 2 cut(s) 71, 232
BssAI RCCGGY 2 cut(s) 71, 232
BssMI GATC 3 cut(s) 42, 292, 342
BstC8I GCNNGC 1 cut(s) 137
BstF5I GGATG 1 cut(s) 51
BstKTI GATC 3 cut(s) 45, 295, 345
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 3 cut(s) 42, 292, 342
BstNSI RCATGY 1 cut(s) 242
BstV1I GCAGC 1 cut(s) 235
BsuRI GGCC 2 cut(s) 18, 176
BtsCI GGATG 1 cut(s) 51
Cac8I GCNNGC 1 cut(s) 137
Cfr10I RCCGGY 2 cut(s) 71, 232
Cfr13I GGNCC 2 cut(s) 17, 107
Csp6I GTAC 1 cut(s) 236
CviAII CATG 3 cut(s) 59, 239, 278
CviJI RGCY 8 cut(s) 18, 28, 139, 166, 176, 195, 232, 248
CviKI_1 RGCY 8 cut(s) 18, 28, 139, 166, 176, 195, 232, 248
CviQI GTAC 1 cut(s) 236
DpnI GATC 3 cut(s) 44, 294, 344
DpnII GATC 3 cut(s) 42, 292, 342
DrdI GACNNNNNNGTC 1 cut(s) 323
DseDI GACNNNNNNGTC 1 cut(s) 323
EaeI YGGCCR 1 cut(s) 174
Eco32I GATATC 1 cut(s) 190
Eco47I GGWCC 1 cut(s) 107
Eco57I CTGAAG 1 cut(s) 44
EcoRV GATATC 1 cut(s) 190
FaeI CATG 3 cut(s) 62, 242, 281
FaiI YATR 6 cut(s) 39, 41, 60, 105, 240, 279
FaqI GGGAC 1 cut(s) 93
FatI CATG 3 cut(s) 58, 238, 277
FauNDI CATATG 1 cut(s) 39
FbaI TGATCA 1 cut(s) 42
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 1 cut(s) 249
FokI GGATG 1 cut(s) 38
Fsp4HI GCNGC 1 cut(s) 249
FspBI CTAG 1 cut(s) 252
GluI GCNGC 1 cut(s) 249
HaeIII GGCC 2 cut(s) 18, 176
HapII CCGG 2 cut(s) 72, 233
Hin1II CATG 3 cut(s) 62, 242, 281
HindIII AAGCTT 1 cut(s) 164
HinfI GANTC 2 cut(s) 261, 284
HpaII CCGG 2 cut(s) 72, 233
HphI GGTGA 3 cut(s) 70, 106, 166
Hpy166II GTNNAC 2 cut(s) 283, 328
Hpy188I TCNGA 1 cut(s) 312
Hpy188III TCNNGA 2 cut(s) 265, 340
Hpy8I GTNNAC 2 cut(s) 283, 328
Hpy99I CGWCG 1 cut(s) 228
HpyAV CCTTC 4 cut(s) 91, 121, 251, 284
Hsp92II CATG 3 cut(s) 62, 242, 281
Ksp22I TGATCA 1 cut(s) 42
Kzo9I GATC 3 cut(s) 42, 292, 342
LmnI GCTCC 1 cut(s) 229
LpnPI CCDG 3 cut(s) 35, 85, 246
Lsp1109I GCAGC 1 cut(s) 235
LweI GCATC 2 cut(s) 84, 316
MaeI CTAG 1 cut(s) 252
MalI GATC 3 cut(s) 44, 294, 344
MboI GATC 3 cut(s) 42, 292, 342
MboII GAAGA 2 cut(s) 119, 344
MhlI GDGCHC 1 cut(s) 204
MlsI TGGCCA 1 cut(s) 176
MluCI AATT 2 cut(s) 170, 182
MluNI TGGCCA 1 cut(s) 176
MlyI GAGTC 1 cut(s) 278
MnlI CCTC 1 cut(s) 143
Mox20I TGGCCA 1 cut(s) 176
MroXI GAANNNNTTC 1 cut(s) 126
MscI TGGCCA 1 cut(s) 176
MseI TTAA 1 cut(s) 358
Msp20I TGGCCA 1 cut(s) 176
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 2 cut(s) 72, 233
NdeI CATATG 1 cut(s) 39
NdeII GATC 3 cut(s) 42, 292, 342
NlaIII CATG 3 cut(s) 62, 242, 281
NlaIV GGNNCC 3 cut(s) 19, 109, 231
NmeAIII GCCGAG 1 cut(s) 111
NspI RCATGY 1 cut(s) 242
PciI ACATGT 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 126
PfeI GAWTC 1 cut(s) 261
PkrI GCNGC 1 cut(s) 250
PleI GAGTC 1 cut(s) 278
PpsI GAGTC 1 cut(s) 278
PscI ACATGT 1 cut(s) 238
PspN4I GGNNCC 3 cut(s) 19, 109, 231
PspPI GGNCC 2 cut(s) 17, 107
RsaI GTAC 1 cut(s) 237
RsaNI GTAC 1 cut(s) 236
SaqAI TTAA 1 cut(s) 358
SatI GCNGC 1 cut(s) 249
Sau3AI GATC 3 cut(s) 42, 292, 342
Sau96I GGNCC 2 cut(s) 17, 107
SchI GAGTC 1 cut(s) 278
SduI GDGCHC 1 cut(s) 204
SetI ASST 5 cut(s) 83, 102, 168, 197, 250
SfaNI GCATC 2 cut(s) 84, 316
SinI GGWCC 1 cut(s) 107
Sse9I AATT 2 cut(s) 170, 182
SsiI CCGC 1 cut(s) 93
SspMI CTAG 1 cut(s) 252
TaqI TCGA 2 cut(s) 186, 223
TasI AATT 2 cut(s) 170, 182
TfiI GAWTC 1 cut(s) 261
Tru1I TTAA 1 cut(s) 358
Tru9I TTAA 1 cut(s) 358
TseI GCWGC 1 cut(s) 248
TspDTI ATGAA 2 cut(s) 135, 266
VpaK11BI GGWCC 1 cut(s) 107
XapI RAATTY 1 cut(s) 182
XceI RCATGY 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 282
XmnI GAANNNNTTC 1 cut(s) 126
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.