RchiOBHm_Chr4g0411091

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003527
Physical Location & Seq
Forward (+)
34861862 .. 34862442
581 bp
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UTR
Exon/CDS
Intron
PRQ38195

Sequence Viewer

Length: 501 bp
ATGTTCGTCTTCTTTGGCCCTCTGAAGCCCGAACCAGACATGATCAAACTCATCTACCATGCCACTGACATCGGGGTCACCTTCCTTGATACCGCTGACCTTTACGATCCCTTCATCAATGAACTTCTTCTCGGTAAGCCTTTGAGTGGAGGGGTGAGAGACAAGCTTGAATTGGCCACCAAATTTGGTATCAGCTTTAAGGACAATAAGAGAGAGATTATTCGAGGGGAGCCGGCATATATGAGAGCTGCTAGTGAAGGAATCTTGAAACGCCTTGGTGTAGACTCTATTAGTCTCTATTATCAGCATCGGATTGACACCCGTGTCCCCATTGAAGTCACGATCTATTTTCTTCTTCAATTTGAACTCTCTGCATCAGGTCATTTCTTCTTCAATTCTTCAATTTTTTGTGGTTTGTGTTTTTTTGTTTTTTTATTTTTAAACTTCATTTTCCAATATTTTTTTGAACTTAATTATCTTTCAATTTTTTTATTGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

166

Amino Acids

19.28

Weight (kDa)

5.9

Isoelectric Point (pI)

25.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 11 - 120 1.5e-15 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 74, 323
AccI GTMKAC 1 cut(s) 282
AciI CCGC 1 cut(s) 93
AclWI GGATC 1 cut(s) 101
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 1 cut(s) 182
AcuI CTGAAG 1 cut(s) 44
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 9 cut(s) 170, 268, 335, 359, 365, 394, 402, 467, 483
AluBI AGCT 3 cut(s) 166, 195, 248
AluI AGCT 3 cut(s) 166, 195, 248
Alw26I GTCTC 2 cut(s) 153, 299
AlwI GGATC 1 cut(s) 101
AoxI GGCC 2 cut(s) 16, 174
ApeKI GCWGC 1 cut(s) 248
ApoI RAATTY 1 cut(s) 182
Asp700I GAANNNNTTC 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 17
AsuHPI GGTGA 2 cut(s) 70, 166
BalI TGGCCA 1 cut(s) 176
BbvI GCAGC 1 cut(s) 235
BclI TGATCA 1 cut(s) 42
BcoDI GTCTC 2 cut(s) 153, 299
BfaI CTAG 1 cut(s) 252
BisI GCNGC 1 cut(s) 249
BlsI GCNGC 1 cut(s) 250
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 1 cut(s) 231
BmsI GCATC 2 cut(s) 316, 383
BsaJI CCNNGG 1 cut(s) 274
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse118I RCCGGY 1 cut(s) 232
BseDI CCNNGG 1 cut(s) 274
BseLI CCNNNNNNNGG 1 cut(s) 146
BseXI GCAGC 1 cut(s) 235
BshFI GGCC 2 cut(s) 18, 176
BsiSI CCGG 1 cut(s) 233
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 2 cut(s) 153, 299
BsmFI GGGAC 1 cut(s) 311
BsnI GGCC 2 cut(s) 18, 176
Bsp143I GATC 3 cut(s) 42, 106, 342
BspACI CCGC 1 cut(s) 93
BspANI GGCC 2 cut(s) 18, 176
BspLI GGNNCC 1 cut(s) 231
BspPI GGATC 1 cut(s) 101
BsrFI RCCGGY 1 cut(s) 232
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 1 cut(s) 274
BssMI GATC 3 cut(s) 42, 106, 342
BssT1I CCWWGG 1 cut(s) 274
BstC8I GCNNGC 1 cut(s) 234
BstEII GGTNACC 1 cut(s) 76
BstKTI GATC 3 cut(s) 45, 109, 345
BstMAI GTCTC 2 cut(s) 153, 299
BstMBI GATC 3 cut(s) 42, 106, 342
BstPI GGTNACC 1 cut(s) 76
BstV1I GCAGC 1 cut(s) 235
BsuRI GGCC 2 cut(s) 18, 176
BtsIMutI CAGTG 1 cut(s) 63
Cac8I GCNNGC 1 cut(s) 234
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 1 cut(s) 17
CspCI CAANNNNNGTGG 2 cut(s) 166, 201
CviAII CATG 2 cut(s) 40, 59
CviJI RGCY 8 cut(s) 18, 28, 139, 166, 176, 195, 232, 248
CviKI_1 RGCY 8 cut(s) 18, 28, 139, 166, 176, 195, 232, 248
DpnI GATC 3 cut(s) 44, 108, 344
DpnII GATC 3 cut(s) 42, 106, 342
DraI TTTAAA 1 cut(s) 441
DrdI GACNNNNNNGTC 2 cut(s) 74, 323
DseDI GACNNNNNNGTC 2 cut(s) 74, 323
EaeI YGGCCR 1 cut(s) 174
Eco130I CCWWGG 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 44
Eco91I GGTNACC 1 cut(s) 76
EcoO65I GGTNACC 1 cut(s) 76
EcoT14I CCWWGG 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 2 cut(s) 43, 62
FaiI YATR 5 cut(s) 41, 60, 238, 240, 242
FaqI GGGAC 1 cut(s) 311
FatI CATG 2 cut(s) 39, 58
FbaI TGATCA 1 cut(s) 42
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 1 cut(s) 249
Fsp4HI GCNGC 1 cut(s) 249
FspBI CTAG 1 cut(s) 252
GluI GCNGC 1 cut(s) 249
HaeIII GGCC 2 cut(s) 18, 176
HapII CCGG 1 cut(s) 233
Hin1II CATG 2 cut(s) 43, 62
HindIII AAGCTT 1 cut(s) 164
HinfI GANTC 2 cut(s) 261, 284
HpaII CCGG 1 cut(s) 233
HphI GGTGA 2 cut(s) 70, 166
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 2 cut(s) 24, 312
Hpy188III TCNNGA 2 cut(s) 265, 340
Hpy8I GTNNAC 1 cut(s) 283
HpyAV CCTTC 3 cut(s) 91, 121, 251
HpyCH4V TGCA 1 cut(s) 374
Hsp92II CATG 2 cut(s) 43, 62
KroI GCCGGC 1 cut(s) 232
KroNI GCCGGC 1 cut(s) 234
Ksp22I TGATCA 1 cut(s) 42
Kzo9I GATC 3 cut(s) 42, 106, 342
LmnI GCTCC 1 cut(s) 229
LpnPI CCDG 3 cut(s) 48, 246, 363
Lsp1109I GCAGC 1 cut(s) 235
LweI GCATC 2 cut(s) 316, 383
MaeI CTAG 1 cut(s) 252
MaeIII GTNAC 2 cut(s) 76, 337
MalI GATC 3 cut(s) 44, 108, 344
MboI GATC 3 cut(s) 42, 106, 342
MboII GAAGA 6 cut(s) 119, 344, 347, 379, 382, 390
MlsI TGGCCA 1 cut(s) 176
MluCI AATT 7 cut(s) 170, 182, 359, 394, 402, 472, 483
MluNI TGGCCA 1 cut(s) 176
MlyI GAGTC 1 cut(s) 278
MnlI CCTC 3 cut(s) 30, 143, 218
Mox20I TGGCCA 1 cut(s) 176
MroNI GCCGGC 1 cut(s) 232
MroXI GAANNNNTTC 1 cut(s) 126
MscI TGGCCA 1 cut(s) 176
MseI TTAA 3 cut(s) 198, 440, 471
Msp20I TGGCCA 1 cut(s) 176
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 1 cut(s) 233
NaeI GCCGGC 1 cut(s) 234
NdeII GATC 3 cut(s) 42, 106, 342
NgoMIV GCCGGC 1 cut(s) 232
NlaIII CATG 2 cut(s) 43, 62
NlaIV GGNNCC 1 cut(s) 231
NmuCI GTSAC 2 cut(s) 76, 337
PdiI GCCGGC 1 cut(s) 234
PdmI GAANNNNTTC 1 cut(s) 126
PfeI GAWTC 1 cut(s) 261
PkrI GCNGC 1 cut(s) 250
PleI GAGTC 1 cut(s) 278
PpsI GAGTC 1 cut(s) 278
PspEI GGTNACC 1 cut(s) 76
PspN4I GGNNCC 1 cut(s) 231
PspPI GGNCC 1 cut(s) 17
SaqAI TTAA 3 cut(s) 198, 440, 471
SatI GCNGC 1 cut(s) 249
Sau3AI GATC 3 cut(s) 42, 106, 342
Sau96I GGNCC 1 cut(s) 17
SchI GAGTC 1 cut(s) 278
SetI ASST 6 cut(s) 83, 102, 168, 197, 250, 382
SfaNI GCATC 2 cut(s) 316, 383
Sse9I AATT 7 cut(s) 170, 182, 359, 394, 402, 472, 483
SsiI CCGC 1 cut(s) 93
SspI AATATT 1 cut(s) 458
SspMI CTAG 1 cut(s) 252
StyI CCWWGG 1 cut(s) 274
TaqI TCGA 1 cut(s) 223
TasI AATT 7 cut(s) 170, 182, 359, 394, 402, 472, 483
TfiI GAWTC 1 cut(s) 261
Tru1I TTAA 3 cut(s) 198, 440, 471
Tru9I TTAA 3 cut(s) 198, 440, 471
TscAI CASTG 1 cut(s) 70
TseFI GTSAC 2 cut(s) 76, 337
TseI GCWGC 1 cut(s) 248
Tsp45I GTSAC 2 cut(s) 76, 337
TspDTI ATGAA 3 cut(s) 103, 135, 436
TspRI CASTG 1 cut(s) 70
XapI RAATTY 1 cut(s) 182
XmiI GTMKAC 1 cut(s) 282
XmnI GAANNNNTTC 1 cut(s) 126
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.