Rroxscaffold_5G00355720

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003527
Physical Location & Seq
Forward (+)
34615056 .. 34616302
1247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00355720.1

Sequence Viewer

Length: 576 bp
ATGTCGGGTCCATCTCTGTTGGTCACTCTGACGTACGTTCTTACGCCATCCCCTCGTTCCATTCTACTCTTCTTTGACCTCACTGCTGACGCCATCACCACCACCAACTGTTTCCTTCACCGAACAGACTTATATATAACTCACCCATGCACCTTCCTTCACTCACTCTGCTACTCTCGATCTGCTTCTGCTCACTCACTCACCGAGAGACATTTAACACATAGACATGGCGGGTGTGAGGAGGATGAAGCTCGGGTCACAAGGCCTGGAGGTGTCAGCTCAAGGACTGGGCTGCATGGGCATGTCAGCCTTCTATGGCCCCCGAAGCCCGAAACCGACATGATCAAGCTCATCCACCATGCCGTCGACTCCGGCGTCACCTTCCTCGACACCTCCGACGTCTACGGTCCCCACACCAACGAAATTCTCCTCGGCAAGGCTTTGAGTGGAGGGGTGAGAGACAAGGTTGAACTGGCCACCAAATGCGGTATCAGGTTTGCGGACAACAAAATGGAGGTTCAAGGCGACCCGGCATATGTTAGAGCTGCTTGTGAAGGAAGCTTGTGGTTTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

191

Amino Acids

20.95

Weight (kDa)

6.44

Isoelectric Point (pI)

39.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 106 - 188 2e-14 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 374
AatII GACGTC 1 cut(s) 402
AccI GTMKAC 2 cut(s) 366, 402
AciI CCGC 3 cut(s) 231, 486, 500
AcoI YGGCCR 1 cut(s) 474
AcsI RAATTY 1 cut(s) 423
AcyI GRCGYC 3 cut(s) 90, 375, 399
AfaI GTAC 1 cut(s) 35
AfiI CCNNNNNNNGG 1 cut(s) 436
AgsI TTSAA 2 cut(s) 470, 521
AjnI CCWGG 1 cut(s) 265
AluBI AGCT 5 cut(s) 251, 279, 349, 545, 561
AluI AGCT 5 cut(s) 251, 279, 349, 545, 561
Alw26I GTCTC 2 cut(s) 202, 453
Ama87I CYCGRG 1 cut(s) 252
AoxI GGCC 3 cut(s) 263, 317, 474
ApeKI GCWGC 2 cut(s) 292, 545
ApoI RAATTY 1 cut(s) 423
AspS9I GGNCC 3 cut(s) 8, 318, 407
AsuC2I CCSGG 1 cut(s) 530
AsuHPI GGTGA 6 cut(s) 88, 110, 134, 193, 370, 466
AvaI CYCGRG 1 cut(s) 252
AvaII GGWCC 2 cut(s) 8, 407
BalI TGGCCA 1 cut(s) 476
BbvI GCAGC 2 cut(s) 279, 532
BccI CCATC 3 cut(s) 19, 55, 101
BceAI ACGGC 1 cut(s) 347
BciT130I CCWGG 1 cut(s) 267
BclI TGATCA 1 cut(s) 342
BcnI CCSGG 1 cut(s) 530
BcoDI GTCTC 2 cut(s) 202, 453
BfaI CTAG 1 cut(s) 574
BisI GCNGC 2 cut(s) 293, 546
BlsI GCNGC 2 cut(s) 294, 547
Bme1390I CCNGG 2 cut(s) 267, 530
Bme18I GGWCC 2 cut(s) 8, 407
BmeT110I CYCGRG 1 cut(s) 252
BmgT120I GGNCC 3 cut(s) 8, 318, 407
BmiI GGNNCC 3 cut(s) 9, 320, 409
BmrFI CCNGG 2 cut(s) 267, 530
BmrI ACTGGG 1 cut(s) 297
BmuI ACTGGG 1 cut(s) 297
BpmI CTGGAG 1 cut(s) 288
BpuEI CTTGAG 1 cut(s) 265
BpuMI CCSGG 1 cut(s) 530
BsaHI GRCGYC 3 cut(s) 90, 375, 399
BsaJI CCNNGG 1 cut(s) 430
Bsc4I CCNNNNNNNGG 1 cut(s) 436
Bse1I ACTGG 2 cut(s) 292, 477
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 1 cut(s) 430
BseGI GGATG 3 cut(s) 47, 250, 351
BseLI CCNNNNNNNGG 1 cut(s) 436
BseNI ACTGG 2 cut(s) 292, 477
BseRI GAGGAG 2 cut(s) 254, 419
BseXI GCAGC 2 cut(s) 279, 532
BshFI GGCC 3 cut(s) 265, 319, 476
BsiHKCI CYCGRG 1 cut(s) 252
BsiSI CCGG 2 cut(s) 372, 530
BsiWI CGTACG 1 cut(s) 33
BslFI GGGAC 1 cut(s) 393
BslI CCNNNNNNNGG 1 cut(s) 436
BsmAI GTCTC 2 cut(s) 202, 453
BsmFI GGGAC 1 cut(s) 393
BsnI GGCC 3 cut(s) 265, 319, 476
BsoBI CYCGRG 1 cut(s) 252
Bsp143I GATC 2 cut(s) 179, 342
BspACI CCGC 3 cut(s) 231, 486, 500
BspANI GGCC 3 cut(s) 265, 319, 476
BspLI GGNNCC 3 cut(s) 9, 320, 409
BsrI ACTGG 2 cut(s) 292, 477
BssECI CCNNGG 1 cut(s) 430
BssMI GATC 2 cut(s) 179, 342
BssNI GRCGYC 3 cut(s) 90, 375, 399
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 2 cut(s) 110, 407
Bst6I CTCTTC 1 cut(s) 74
BstACI GRCGYC 3 cut(s) 90, 375, 399
BstENI CCTNNNNNAGG 1 cut(s) 434
BstF5I GGATG 3 cut(s) 47, 250, 351
BstKTI GATC 2 cut(s) 182, 345
BstMAI GTCTC 2 cut(s) 202, 453
BstMBI GATC 2 cut(s) 179, 342
BstMWI GCNNNNNNNGC 2 cut(s) 298, 325
BstNI CCWGG 1 cut(s) 267
BstNSI RCATGY 1 cut(s) 305
BstSCI CCNGG 2 cut(s) 265, 528
BstV1I GCAGC 2 cut(s) 279, 532
BsuRI GGCC 3 cut(s) 265, 319, 476
BtsCI GGATG 3 cut(s) 47, 250, 351
BtsI GCAGTG 1 cut(s) 81
BtsIMutI CAGTG 1 cut(s) 81
Cfr13I GGNCC 3 cut(s) 8, 318, 407
CseI GACGC 2 cut(s) 98, 364
Csp6I GTAC 1 cut(s) 34
CviAII CATG 6 cut(s) 147, 227, 296, 302, 340, 359
CviQI GTAC 1 cut(s) 34
DpnI GATC 2 cut(s) 181, 344
DpnII GATC 2 cut(s) 179, 342
DrdI GACNNNNNNGTC 1 cut(s) 374
DseDI GACNNNNNNGTC 1 cut(s) 374
EaeI YGGCCR 1 cut(s) 474
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Eco147I AGGCCT 1 cut(s) 265
Eco47I GGWCC 2 cut(s) 8, 407
Eco88I CYCGRG 1 cut(s) 252
EcoNI CCTNNNNNAGG 1 cut(s) 434
EcoRII CCWGG 1 cut(s) 265
FaeI CATG 6 cut(s) 150, 230, 299, 305, 343, 362
FaqI GGGAC 1 cut(s) 393
FatI CATG 6 cut(s) 146, 226, 295, 301, 339, 358
FauI CCCGC 1 cut(s) 224
FauNDI CATATG 1 cut(s) 535
FbaI TGATCA 1 cut(s) 342
FblI GTMKAC 2 cut(s) 366, 402
Fnu4HI GCNGC 2 cut(s) 293, 546
FokI GGATG 3 cut(s) 34, 257, 338
Fsp4HI GCNGC 2 cut(s) 293, 546
FspBI CTAG 1 cut(s) 574
GluI GCNGC 2 cut(s) 293, 546
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 3 cut(s) 265, 319, 476
HapII CCGG 2 cut(s) 372, 530
HgaI GACGC 2 cut(s) 98, 364
Hin1I GRCGYC 3 cut(s) 90, 375, 399
Hin1II CATG 6 cut(s) 150, 230, 299, 305, 343, 362
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HindIII AAGCTT 1 cut(s) 559
HinfI GANTC 1 cut(s) 368
HpaII CCGG 2 cut(s) 372, 530
HphI GGTGA 6 cut(s) 88, 110, 134, 193, 370, 466
Hpy166II GTNNAC 2 cut(s) 367, 403
Hpy188I TCNGA 2 cut(s) 30, 397
Hpy188III TCNNGA 1 cut(s) 177
Hpy8I GTNNAC 2 cut(s) 367, 403
Hpy99I CGWCG 2 cut(s) 368, 401
HpyAV CCTTC 6 cut(s) 125, 163, 167, 320, 391, 548
HpyCH4III ACNGT 2 cut(s) 110, 407
HpyCH4IV ACGT 3 cut(s) 32, 36, 399
HpyCH4V TGCA 2 cut(s) 150, 295
HpyF10VI GCNNNNNNNGC 2 cut(s) 298, 325
HpySE526I ACGT 3 cut(s) 32, 36, 399
Hsp92I GRCGYC 3 cut(s) 90, 375, 399
Hsp92II CATG 6 cut(s) 150, 230, 299, 305, 343, 362
Ksp22I TGATCA 1 cut(s) 342
Kzo9I GATC 2 cut(s) 179, 342
LpnPI CCDG 7 cut(s) 252, 273, 279, 385, 458, 478, 543
Lsp1109I GCAGC 2 cut(s) 279, 532
MaeI CTAG 1 cut(s) 574
MaeII ACGT 3 cut(s) 32, 36, 399
MaeIII GTNAC 3 cut(s) 22, 256, 376
MalI GATC 2 cut(s) 181, 344
MboI GATC 2 cut(s) 179, 342
MboII GAAGA 1 cut(s) 61
MlsI TGGCCA 1 cut(s) 476
MluCI AATT 1 cut(s) 423
MluNI TGGCCA 1 cut(s) 476
MlyI GAGTC 1 cut(s) 362
MmeI TCCRAC 1 cut(s) 420
Mox20I TGGCCA 1 cut(s) 476
MscI TGGCCA 1 cut(s) 476
MseI TTAA 1 cut(s) 215
MslI CAYNNNNRTG 2 cut(s) 225, 300
Msp20I TGGCCA 1 cut(s) 476
MspI CCGG 2 cut(s) 372, 530
MspR9I CCNGG 2 cut(s) 267, 530
MvaI CCWGG 1 cut(s) 267
MwoI GCNNNNNNNGC 2 cut(s) 298, 325
NciI CCSGG 1 cut(s) 530
NdeI CATATG 1 cut(s) 535
NdeII GATC 2 cut(s) 179, 342
NlaIII CATG 6 cut(s) 150, 230, 299, 305, 343, 362
NlaIV GGNNCC 3 cut(s) 9, 320, 409
NmeAIII GCCGAG 1 cut(s) 411
NmuCI GTSAC 3 cut(s) 22, 256, 376
NspI RCATGY 1 cut(s) 305
PceI AGGCCT 1 cut(s) 265
PcsI WCGNNNNNNNCGW 2 cut(s) 372, 393
Pfl23II CGTACG 1 cut(s) 33
PkrI GCNGC 2 cut(s) 294, 547
PleI GAGTC 1 cut(s) 362
PpsI GAGTC 1 cut(s) 362
Psp6I CCWGG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 265
PspLI CGTACG 1 cut(s) 33
PspN4I GGNNCC 3 cut(s) 9, 320, 409
PspPI GGNCC 3 cut(s) 8, 318, 407
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
RseI CAYNNNNRTG 2 cut(s) 225, 300
SalI GTCGAC 1 cut(s) 365
SaqAI TTAA 1 cut(s) 215
SatI GCNGC 2 cut(s) 293, 546
Sau3AI GATC 2 cut(s) 179, 342
Sau96I GGNCC 3 cut(s) 8, 318, 407
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 2 cut(s) 267, 530
SinI GGWCC 2 cut(s) 8, 407
SmiMI CAYNNNNRTG 2 cut(s) 225, 300
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
Sse9I AATT 1 cut(s) 423
SseBI AGGCCT 1 cut(s) 265
SsiI CCGC 3 cut(s) 231, 486, 500
SspMI CTAG 1 cut(s) 574
StuI AGGCCT 1 cut(s) 265
StyD4I CCNGG 2 cut(s) 265, 528
TaaI ACNGT 2 cut(s) 110, 407
TaiI ACGT 3 cut(s) 35, 39, 402
TaqI TCGA 3 cut(s) 178, 366, 387
TasI AATT 1 cut(s) 423
Tru1I TTAA 1 cut(s) 215
Tru9I TTAA 1 cut(s) 215
TscAI CASTG 1 cut(s) 88
TseFI GTSAC 3 cut(s) 22, 256, 376
TseI GCWGC 2 cut(s) 292, 545
Tsp45I GTSAC 3 cut(s) 22, 256, 376
TspDTI ATGAA 1 cut(s) 261
TspRI CASTG 1 cut(s) 88
VpaK11BI GGWCC 2 cut(s) 8, 407
XagI CCTNNNNNAGG 1 cut(s) 434
XapI RAATTY 1 cut(s) 423
XceI RCATGY 1 cut(s) 305
XmiI GTMKAC 2 cut(s) 366, 402
XspI CTAG 1 cut(s) 574
ZraI GACGTC 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.