Rh4AG164000

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003527
Physical Location & Seq
Forward (+)
41499749 .. 41518710
18962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG164000.1

Sequence Viewer

Length: 420 bp
ATGGCGGGCGTGAGGAGGATCAAGCTCGGGTCACAGGGCCTGGAGGTCTCAGCTCAAGGACTCGGCTGCATGGGCATGTCCTCCTTCTATGGGCCCCCGAAGCCCGAAGCCGACATGATCAAGCTCATCCGCCACGCCGTCGACTCAGGCGTCACCTTCCTCGACACCTCCGACATCTACGGCCCGCACACCAACGAAATTCTCCTCGGCGAAGCTTTGAGTGGAGGGGTGAGAGACAAGGTTGAATTGGCCACGAAATTTGGTATCAGCTTTGAGGACAACAAGAGAGAGGTTCGGGGCGATCCGGCTTATGTCAGGTCTGCTTGTGAAGGAAGCTTGAAACGCCTCGGTGTTAATTGTGTGGATCTCTATTATCAGCATCGGATCGACATTCGTCTCCCCATTGAAGTCACGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

139

Amino Acids

15.16

Weight (kDa)

6.29

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 136 9.8e-25 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
AccI GTMKAC 1 cut(s) 141
AciI CCGC 3 cut(s) 5, 130, 185
AclWI GGATC 4 cut(s) 26, 296, 372, 392
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 2 cut(s) 198, 257
AcyI GRCGYC 1 cut(s) 150
AfiI CCNNNNNNNGG 1 cut(s) 90
AgsI TTSAA 3 cut(s) 245, 340, 407
AjiI CACGTC 1 cut(s) 414
AjnI CCWGG 1 cut(s) 39
AluBI AGCT 6 cut(s) 25, 53, 124, 215, 270, 336
AluI AGCT 6 cut(s) 25, 53, 124, 215, 270, 336
Alw26I GTCTC 3 cut(s) 52, 228, 401
AlwI GGATC 4 cut(s) 26, 296, 372, 392
AlwNI CAGNNNCTG 1 cut(s) 40
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 4 cut(s) 37, 92, 181, 249
ApaI GGGCCC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 66
ApoI RAATTY 2 cut(s) 198, 257
AspS9I GGNCC 4 cut(s) 37, 92, 93, 182
AsuHPI GGTGA 2 cut(s) 145, 241
AvaI CYCGRG 1 cut(s) 26
BaeGI GKGCMC 1 cut(s) 96
BalI TGGCCA 1 cut(s) 251
BanII GRGCYC 1 cut(s) 96
BbvI GCAGC 1 cut(s) 53
BceAI ACGGC 2 cut(s) 122, 196
BciT130I CCWGG 1 cut(s) 41
BclI TGATCA 1 cut(s) 117
BcoDI GTCTC 3 cut(s) 52, 228, 401
BisI GCNGC 1 cut(s) 67
BlsI GCNGC 1 cut(s) 68
Bme1390I CCNGG 1 cut(s) 41
BmeT110I CYCGRG 1 cut(s) 26
BmgBI CACGTC 1 cut(s) 414
BmgT120I GGNCC 4 cut(s) 37, 92, 93, 182
BmiI GGNNCC 2 cut(s) 94, 95
BmrFI CCNGG 1 cut(s) 41
BmsI GCATC 1 cut(s) 388
BoxI GACNNNNGTC 1 cut(s) 393
BpmI CTGGAG 1 cut(s) 62
BpuEI CTTGAG 1 cut(s) 39
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 52
BsaJI CCNNGG 2 cut(s) 205, 346
Bsc4I CCNNNNNNNGG 1 cut(s) 90
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 2 cut(s) 205, 346
BseGI GGATG 1 cut(s) 126
BseLI CCNNNNNNNGG 1 cut(s) 90
BseMII CTCAG 2 cut(s) 63, 159
BseRI GAGGAG 2 cut(s) 28, 194
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 1 cut(s) 53
BshFI GGCC 4 cut(s) 39, 94, 183, 251
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 1 cut(s) 305
BslI CCNNNNNNNGG 1 cut(s) 90
BsmAI GTCTC 3 cut(s) 52, 228, 401
BsmBI CGTCTC 1 cut(s) 401
BsnI GGCC 4 cut(s) 39, 94, 183, 251
Bso31I GGTCTC 1 cut(s) 52
BsoBI CYCGRG 1 cut(s) 26
Bsp120I GGGCCC 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 5 cut(s) 18, 117, 301, 364, 384
BspACI CCGC 3 cut(s) 5, 130, 185
BspANI GGCC 4 cut(s) 39, 94, 183, 251
BspCNI CTCAG 2 cut(s) 62, 158
BspLI GGNNCC 2 cut(s) 94, 95
BspPI GGATC 4 cut(s) 26, 296, 372, 392
BspTNI GGTCTC 1 cut(s) 52
BssECI CCNNGG 2 cut(s) 205, 346
BssMI GATC 5 cut(s) 18, 117, 301, 364, 384
BssNI GRCGYC 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 41
BstACI GRCGYC 1 cut(s) 150
BstC8I GCNNGC 2 cut(s) 7, 185
BstDEI CTNAG 2 cut(s) 49, 145
BstF5I GGATG 1 cut(s) 126
BstKTI GATC 5 cut(s) 21, 120, 304, 367, 387
BstMAI GTCTC 3 cut(s) 52, 228, 401
BstMBI GATC 5 cut(s) 18, 117, 301, 364, 384
BstMWI GCNNNNNNNGC 3 cut(s) 72, 100, 342
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 1 cut(s) 79
BstPAI GACNNNNGTC 1 cut(s) 393
BstSCI CCNGG 1 cut(s) 39
BstSLI GKGCMC 1 cut(s) 96
BstV1I GCAGC 1 cut(s) 53
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BsuRI GGCC 4 cut(s) 39, 94, 183, 251
BtrI CACGTC 1 cut(s) 414
BtsCI GGATG 1 cut(s) 126
Cac8I GCNNGC 2 cut(s) 7, 185
CaiI CAGNNNCTG 1 cut(s) 40
Cfr13I GGNCC 4 cut(s) 37, 92, 93, 182
CseI GACGC 1 cut(s) 139
CspCI CAANNNNNGTGG 2 cut(s) 241, 276
CviAII CATG 3 cut(s) 70, 76, 115
DdeI CTNAG 2 cut(s) 49, 145
DpnI GATC 5 cut(s) 20, 119, 303, 366, 386
DpnII GATC 5 cut(s) 18, 117, 301, 364, 384
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 1 cut(s) 249
EciI GGCGGA 1 cut(s) 119
Eco24I GRGCYC 1 cut(s) 96
Eco31I GGTCTC 1 cut(s) 52
Eco88I CYCGRG 1 cut(s) 26
EcoO109I RGGNCCY 2 cut(s) 37, 93
EcoRII CCWGG 1 cut(s) 39
EcoT38I GRGCYC 1 cut(s) 96
Esp3I CGTCTC 1 cut(s) 401
FaeI CATG 3 cut(s) 73, 79, 118
FaiI YATR 5 cut(s) 71, 77, 90, 116, 312
FatI CATG 3 cut(s) 69, 75, 114
FauI CCCGC 1 cut(s) 192
FbaI TGATCA 1 cut(s) 117
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 1 cut(s) 67
FokI GGATG 1 cut(s) 113
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 67
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 4 cut(s) 39, 94, 183, 251
HapII CCGG 1 cut(s) 305
HgaI GACGC 1 cut(s) 139
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 3 cut(s) 73, 79, 118
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HindIII AAGCTT 2 cut(s) 213, 334
HinfI GANTC 2 cut(s) 60, 143
HpaII CCGG 1 cut(s) 305
HphI GGTGA 2 cut(s) 145, 241
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 2 cut(s) 172, 384
Hpy8I GTNNAC 1 cut(s) 142
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 3 cut(s) 94, 166, 323
HpyCH4IV ACGT 1 cut(s) 413
HpyCH4V TGCA 1 cut(s) 69
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 100, 342
HpyF3I CTNAG 2 cut(s) 49, 145
HpySE526I ACGT 1 cut(s) 413
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 3 cut(s) 73, 79, 118
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 5 cut(s) 18, 117, 301, 364, 384
LpnPI CCDG 6 cut(s) 20, 26, 53, 132, 301, 318
Lsp1109I GCAGC 1 cut(s) 53
LweI GCATC 1 cut(s) 388
MaeII ACGT 1 cut(s) 413
MaeIII GTNAC 3 cut(s) 30, 151, 409
MalI GATC 5 cut(s) 20, 119, 303, 366, 386
MboI GATC 5 cut(s) 18, 117, 301, 364, 384
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 1 cut(s) 96
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 4 cut(s) 198, 245, 257, 355
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 2 cut(s) 54, 137
MmeI TCCRAC 1 cut(s) 195
Mox20I TGGCCA 1 cut(s) 251
MscI TGGCCA 1 cut(s) 251
MseI TTAA 2 cut(s) 354, 418
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 251
MspI CCGG 1 cut(s) 305
MspR9I CCNGG 1 cut(s) 41
MvaI CCWGG 1 cut(s) 41
MwoI GCNNNNNNNGC 3 cut(s) 72, 100, 342
NdeII GATC 5 cut(s) 18, 117, 301, 364, 384
NlaIII CATG 3 cut(s) 73, 79, 118
NlaIV GGNNCC 2 cut(s) 94, 95
NmeAIII GCCGAG 2 cut(s) 42, 186
NmuCI GTSAC 3 cut(s) 30, 151, 409
NspI RCATGY 1 cut(s) 79
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 168
PkrI GCNGC 1 cut(s) 68
PleI GAGTC 2 cut(s) 54, 137
PpsI GAGTC 2 cut(s) 54, 137
PshAI GACNNNNGTC 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 39
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 2 cut(s) 94, 95
PspOMI GGGCCC 1 cut(s) 92
PspPI GGNCC 4 cut(s) 37, 92, 93, 182
PstNI CAGNNNCTG 1 cut(s) 40
PsuI RGATCY 1 cut(s) 364
RseI CAYNNNNRTG 1 cut(s) 74
SalI GTCGAC 1 cut(s) 140
SaqAI TTAA 2 cut(s) 354, 418
SatI GCNGC 1 cut(s) 67
Sau3AI GATC 5 cut(s) 18, 117, 301, 364, 384
Sau96I GGNCC 4 cut(s) 37, 92, 93, 182
SchI GAGTC 2 cut(s) 54, 137
ScrFI CCNGG 1 cut(s) 41
SduI GDGCHC 1 cut(s) 96
SfaNI GCATC 1 cut(s) 388
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
Sse9I AATT 4 cut(s) 198, 245, 257, 355
SsiI CCGC 3 cut(s) 5, 130, 185
StyD4I CCNGG 1 cut(s) 39
TaiI ACGT 1 cut(s) 416
TaqI TCGA 3 cut(s) 141, 162, 387
TasI AATT 4 cut(s) 198, 245, 257, 355
Tru1I TTAA 2 cut(s) 354, 418
Tru9I TTAA 2 cut(s) 354, 418
TseFI GTSAC 3 cut(s) 30, 151, 409
TseI GCWGC 1 cut(s) 66
Tsp45I GTSAC 3 cut(s) 30, 151, 409
XapI RAATTY 2 cut(s) 198, 257
XceI RCATGY 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.