Rh4AG166000

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003527
Physical Location & Seq
Forward (+)
41851622 .. 41852114
493 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG166000.1

Sequence Viewer

Length: 420 bp
ATGGCGGGTGTGAGGAGGATGAAGCTCGGGTCACAAGGCCTGGAGGTCTCAGCACAAGGATTGGGCTGCATGGGCATGTCAGCCTTCTATGGCCCCCCGAAGCCCGAAGCCGACATGATCAAGCTCATCCACCATGCTGTCGACTCCGGCGTCACCTTCCTCGACACTTCCGACGTCAACGGTCCCCACACCAACGAAATTCTCCTCGGCAAGGCTTTGAGTGGAGGGGTGAGAGACAAGGTTGAACTGGCCACCAAATGCGGTATCAGGTTTGCGGACAACAAAATGGAGGTTCAAGGCGACCCGGCATATGTGAGAGCTGCTTGTGAAGGAAGCTTGAAACGCCTTGGTGTTGCTTCTGTTGATCTCTATTATCAGCATAGGATTGACACCCGTGTCCCCATTGAAGTCACGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

139

Amino Acids

14.9

Weight (kDa)

6.89

Isoelectric Point (pI)

34.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 138 3.5e-23 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 149, 395
AatII GACGTC 1 cut(s) 177
AccI GTMKAC 1 cut(s) 141
AciI CCGC 3 cut(s) 5, 261, 275
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 1 cut(s) 198
AcyI GRCGYC 2 cut(s) 150, 174
AfiI CCNNNNNNNGG 1 cut(s) 211
AgsI TTSAA 4 cut(s) 245, 296, 340, 407
AjnI CCWGG 1 cut(s) 39
AluBI AGCT 4 cut(s) 25, 124, 320, 336
AluI AGCT 4 cut(s) 25, 124, 320, 336
Alw26I GTCTC 2 cut(s) 52, 228
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 3 cut(s) 37, 91, 249
ApeKI GCWGC 2 cut(s) 66, 320
ApoI RAATTY 1 cut(s) 198
AspS9I GGNCC 2 cut(s) 92, 182
AsuC2I CCSGG 1 cut(s) 305
AsuHPI GGTGA 2 cut(s) 145, 241
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 1 cut(s) 182
BalI TGGCCA 1 cut(s) 251
BbvI GCAGC 2 cut(s) 53, 307
BciT130I CCWGG 1 cut(s) 41
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 1 cut(s) 305
BcoDI GTCTC 2 cut(s) 52, 228
BisI GCNGC 2 cut(s) 67, 321
BlsI GCNGC 2 cut(s) 68, 322
Bme1390I CCNGG 2 cut(s) 41, 305
Bme18I GGWCC 1 cut(s) 182
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 2 cut(s) 92, 182
BmiI GGNNCC 2 cut(s) 94, 184
BmrFI CCNGG 2 cut(s) 41, 305
BpmI CTGGAG 1 cut(s) 62
BpuMI CCSGG 1 cut(s) 305
BsaHI GRCGYC 2 cut(s) 150, 174
BsaI GGTCTC 1 cut(s) 52
BsaJI CCNNGG 2 cut(s) 205, 346
Bsc4I CCNNNNNNNGG 1 cut(s) 211
Bse1I ACTGG 1 cut(s) 252
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 2 cut(s) 205, 346
BseGI GGATG 2 cut(s) 24, 126
BseLI CCNNNNNNNGG 1 cut(s) 211
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 1 cut(s) 252
BseRI GAGGAG 2 cut(s) 28, 194
BseXI GCAGC 2 cut(s) 53, 307
BshFI GGCC 3 cut(s) 39, 93, 251
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 2 cut(s) 147, 305
BslFI GGGAC 2 cut(s) 168, 383
BslI CCNNNNNNNGG 1 cut(s) 211
BsmAI GTCTC 2 cut(s) 52, 228
BsmFI GGGAC 2 cut(s) 168, 383
BsnI GGCC 3 cut(s) 39, 93, 251
Bso31I GGTCTC 1 cut(s) 52
BsoBI CYCGRG 1 cut(s) 26
Bsp143I GATC 2 cut(s) 117, 364
BspACI CCGC 3 cut(s) 5, 261, 275
BspANI GGCC 3 cut(s) 39, 93, 251
BspCNI CTCAG 1 cut(s) 62
BspLI GGNNCC 2 cut(s) 94, 184
BspTNI GGTCTC 1 cut(s) 52
BsrI ACTGG 1 cut(s) 252
BssECI CCNNGG 2 cut(s) 205, 346
BssMI GATC 2 cut(s) 117, 364
BssNI GRCGYC 2 cut(s) 150, 174
BssT1I CCWWGG 1 cut(s) 346
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 2 cut(s) 182, 415
BstACI GRCGYC 2 cut(s) 150, 174
BstDEI CTNAG 1 cut(s) 49
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 2 cut(s) 24, 126
BstKTI GATC 2 cut(s) 120, 367
BstMAI GTCTC 2 cut(s) 52, 228
BstMBI GATC 2 cut(s) 117, 364
BstMWI GCNNNNNNNGC 2 cut(s) 72, 342
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 1 cut(s) 79
BstSCI CCNGG 2 cut(s) 39, 303
BstV1I GCAGC 2 cut(s) 53, 307
BsuRI GGCC 3 cut(s) 39, 93, 251
BtsCI GGATG 2 cut(s) 24, 126
Cfr13I GGNCC 2 cut(s) 92, 182
CseI GACGC 1 cut(s) 139
CviAII CATG 4 cut(s) 70, 76, 115, 134
DdeI CTNAG 1 cut(s) 49
DpnI GATC 2 cut(s) 119, 366
DpnII GATC 2 cut(s) 117, 364
DrdI GACNNNNNNGTC 2 cut(s) 149, 395
DseDI GACNNNNNNGTC 2 cut(s) 149, 395
EaeI YGGCCR 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 346
Eco147I AGGCCT 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 182
Eco88I CYCGRG 1 cut(s) 26
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoRII CCWGG 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 346
FaeI CATG 4 cut(s) 73, 79, 118, 137
FaiI YATR 8 cut(s) 71, 77, 90, 116, 135, 310, 312, 381
FaqI GGGAC 2 cut(s) 168, 383
FatI CATG 4 cut(s) 69, 75, 114, 133
FauNDI CATATG 1 cut(s) 310
FbaI TGATCA 1 cut(s) 117
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 2 cut(s) 67, 321
FokI GGATG 2 cut(s) 31, 113
Fsp4HI GCNGC 2 cut(s) 67, 321
GluI GCNGC 2 cut(s) 67, 321
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 3 cut(s) 39, 93, 251
HapII CCGG 2 cut(s) 147, 305
HgaI GACGC 1 cut(s) 139
Hin1I GRCGYC 2 cut(s) 150, 174
Hin1II CATG 4 cut(s) 73, 79, 118, 137
HincII GTYRAC 2 cut(s) 142, 178
HindII GTYRAC 2 cut(s) 142, 178
HindIII AAGCTT 1 cut(s) 334
HinfI GANTC 1 cut(s) 143
HpaII CCGG 2 cut(s) 147, 305
HphI GGTGA 2 cut(s) 145, 241
Hpy166II GTNNAC 2 cut(s) 142, 178
Hpy188I TCNGA 1 cut(s) 172
Hpy8I GTNNAC 2 cut(s) 142, 178
Hpy99I CGWCG 1 cut(s) 176
HpyAV CCTTC 3 cut(s) 94, 166, 323
HpyCH4III ACNGT 2 cut(s) 182, 415
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 1 cut(s) 69
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 342
HpyF3I CTNAG 1 cut(s) 49
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 2 cut(s) 150, 174
Hsp92II CATG 4 cut(s) 73, 79, 118, 137
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 2 cut(s) 117, 364
LpnPI CCDG 6 cut(s) 26, 53, 160, 233, 253, 318
Lsp1109I GCAGC 2 cut(s) 53, 307
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 3 cut(s) 30, 151, 409
MalI GATC 2 cut(s) 119, 366
MboI GATC 2 cut(s) 117, 364
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 1 cut(s) 198
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 7 cut(s) 6, 9, 37, 170, 215, 218, 283
Mox20I TGGCCA 1 cut(s) 251
MscI TGGCCA 1 cut(s) 251
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 251
MspI CCGG 2 cut(s) 147, 305
MspR9I CCNGG 2 cut(s) 41, 305
MvaI CCWGG 1 cut(s) 41
MwoI GCNNNNNNNGC 2 cut(s) 72, 342
NciI CCSGG 1 cut(s) 305
NdeI CATATG 1 cut(s) 310
NdeII GATC 2 cut(s) 117, 364
NlaIII CATG 4 cut(s) 73, 79, 118, 137
NlaIV GGNNCC 2 cut(s) 94, 184
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 3 cut(s) 30, 151, 409
NspI RCATGY 1 cut(s) 79
PceI AGGCCT 1 cut(s) 39
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 168
PkrI GCNGC 2 cut(s) 68, 322
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
Psp6I CCWGG 1 cut(s) 39
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 2 cut(s) 94, 184
PspPI GGNCC 2 cut(s) 92, 182
RseI CAYNNNNRTG 1 cut(s) 74
SalI GTCGAC 1 cut(s) 140
SatI GCNGC 2 cut(s) 67, 321
Sau3AI GATC 2 cut(s) 117, 364
Sau96I GGNCC 2 cut(s) 92, 182
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 2 cut(s) 41, 305
SinI GGWCC 1 cut(s) 182
SmiMI CAYNNNNRTG 1 cut(s) 74
Sse9I AATT 1 cut(s) 198
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 3 cut(s) 5, 261, 275
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 39, 303
StyI CCWWGG 1 cut(s) 346
TaaI ACNGT 2 cut(s) 182, 415
TaiI ACGT 1 cut(s) 177
TaqI TCGA 2 cut(s) 141, 162
TasI AATT 1 cut(s) 198
TseFI GTSAC 3 cut(s) 30, 151, 409
TseI GCWGC 2 cut(s) 66, 320
Tsp45I GTSAC 3 cut(s) 30, 151, 409
TspDTI ATGAA 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 182
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 1 cut(s) 198
XceI RCATGY 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 141
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.