Rh4AG163200

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003527
Physical Location & Seq
Reverse (-)
41317019 .. 41321962
4944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG163200.1

Sequence Viewer

Length: 504 bp
ATGGCGAGCATCAGGAGGATGAAGCTGGGGTCAGAAGGCCTGGAGGTCTCGGCTCAAGGGCTGGGTTGCGTGGGCATGTCCGTCTTCTCTGGCCCCCCGAAGCCTGAAGCCGACATGATCAAGCTCATCCACCACGCCATCAACGCCGGCGTCACCTTCCTTGATACCGCTGACGTTTACGGTCCCTTCACCAACGAACTTCTTCTTGGCAAGGCTTTGAGTGGAGGGGTGAGAGACAAGGTTGAATTGGCCACCAAATTTGGTATCAGCTTTGAGGACAACAAGAGAGAGATTATTCGAGGCGACCCGGCATATGTCAGGTCTGCTTGTGAAGGAAGCTTGAAACGACTTGGTGTAGATTCTATTGATCTCTATTATCAGCATCGGATTGACACTCGTGTCCCCATCGAAGTCACGGTTTGTTTTCTTCTTCAATTTGATCTCTCAATGTCGTTTGTTTGCTTGAACTCAGTTTTGCTAGTGTCTCAAATCTTAACGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

167

Amino Acids

18.23

Weight (kDa)

5.9

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 24 - 143 7.2e-23 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 398
AciI CCGC 1 cut(s) 168
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 1 cut(s) 257
AcuI CTGAAG 1 cut(s) 126
AcyI GRCGYC 1 cut(s) 150
AgsI TTSAA 4 cut(s) 245, 343, 434, 466
AjnI CCWGG 1 cut(s) 39
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AluBI AGCT 4 cut(s) 25, 124, 270, 339
AluI AGCT 4 cut(s) 25, 124, 270, 339
Alw26I GTCTC 3 cut(s) 52, 228, 489
AoxI GGCC 3 cut(s) 37, 91, 249
ApoI RAATTY 1 cut(s) 257
Asp700I GAANNNNTTC 1 cut(s) 201
AspS9I GGNCC 2 cut(s) 92, 182
AsuC2I CCSGG 1 cut(s) 308
AsuHPI GGTGA 3 cut(s) 145, 181, 241
AvaII GGWCC 1 cut(s) 182
BalI TGGCCA 1 cut(s) 251
BauI CACGAG 1 cut(s) 396
BbsI GAAGAC 1 cut(s) 76
BccI CCATC 2 cut(s) 146, 413
BciT130I CCWGG 1 cut(s) 41
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 1 cut(s) 308
BcoDI GTCTC 3 cut(s) 52, 228, 489
BfaI CTAG 1 cut(s) 479
Bme1390I CCNGG 2 cut(s) 41, 308
Bme18I GGWCC 1 cut(s) 182
BmgT120I GGNCC 2 cut(s) 92, 182
BmiI GGNNCC 2 cut(s) 94, 184
BmrFI CCNGG 2 cut(s) 41, 308
BmsI GCATC 2 cut(s) 18, 391
BpiI GAAGAC 1 cut(s) 76
BpmI CTGGAG 1 cut(s) 62
BpuEI CTTGAG 1 cut(s) 39
BpuMI CCSGG 1 cut(s) 308
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 52
Bse118I RCCGGY 1 cut(s) 146
BseBI CCWGG 1 cut(s) 41
BseGI GGATG 2 cut(s) 24, 126
BseMII CTCAG 1 cut(s) 483
BseYI CCCAGC 2 cut(s) 25, 61
BshFI GGCC 3 cut(s) 39, 93, 251
BsiSI CCGG 2 cut(s) 147, 308
BslFI GGGAC 2 cut(s) 168, 386
BsmAI GTCTC 3 cut(s) 52, 228, 489
BsmFI GGGAC 2 cut(s) 168, 386
BsnI GGCC 3 cut(s) 39, 93, 251
Bso31I GGTCTC 1 cut(s) 52
Bsp143I GATC 3 cut(s) 117, 367, 439
BspACI CCGC 1 cut(s) 168
BspANI GGCC 3 cut(s) 39, 93, 251
BspCNI CTCAG 1 cut(s) 482
BspLI GGNNCC 2 cut(s) 94, 184
BspTNI GGTCTC 1 cut(s) 52
BsrFI RCCGGY 1 cut(s) 146
BssAI RCCGGY 1 cut(s) 146
BssMI GATC 3 cut(s) 117, 367, 439
BssNI GRCGYC 1 cut(s) 150
BssSI CACGAG 1 cut(s) 396
Bst2BI CACGAG 1 cut(s) 396
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 2 cut(s) 182, 418
BstACI GRCGYC 1 cut(s) 150
BstC8I GCNNGC 2 cut(s) 7, 148
BstDEI CTNAG 1 cut(s) 469
BstF5I GGATG 2 cut(s) 24, 126
BstKTI GATC 3 cut(s) 120, 370, 442
BstMAI GTCTC 3 cut(s) 52, 228, 489
BstMBI GATC 3 cut(s) 117, 367, 439
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 1 cut(s) 79
BstSCI CCNGG 2 cut(s) 39, 306
BstV2I GAAGAC 1 cut(s) 76
BsuRI GGCC 3 cut(s) 39, 93, 251
BtsCI GGATG 2 cut(s) 24, 126
Cac8I GCNNGC 2 cut(s) 7, 148
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 2 cut(s) 92, 182
CseI GACGC 1 cut(s) 139
CspCI CAANNNNNGTGG 2 cut(s) 241, 276
CviAII CATG 2 cut(s) 76, 115
DdeI CTNAG 1 cut(s) 469
DpnI GATC 3 cut(s) 119, 369, 441
DpnII GATC 3 cut(s) 117, 367, 439
DrdI GACNNNNNNGTC 1 cut(s) 398
DseDI GACNNNNNNGTC 1 cut(s) 398
EaeI YGGCCR 1 cut(s) 249
Eco147I AGGCCT 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 126
EcoRII CCWGG 1 cut(s) 39
FaeI CATG 2 cut(s) 79, 118
FaiI YATR 4 cut(s) 77, 116, 313, 315
FaqI GGGAC 2 cut(s) 168, 386
FatI CATG 2 cut(s) 75, 114
FauNDI CATATG 1 cut(s) 313
FbaI TGATCA 1 cut(s) 117
FokI GGATG 2 cut(s) 31, 113
FspBI CTAG 1 cut(s) 479
GsaI CCCAGC 2 cut(s) 29, 65
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 3 cut(s) 39, 93, 251
HapII CCGG 2 cut(s) 147, 308
HgaI GACGC 1 cut(s) 139
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 2 cut(s) 79, 118
HindIII AAGCTT 1 cut(s) 337
HinfI GANTC 1 cut(s) 359
HpaII CCGG 2 cut(s) 147, 308
HphI GGTGA 3 cut(s) 145, 181, 241
Hpy166II GTNNAC 1 cut(s) 178
Hpy188I TCNGA 2 cut(s) 34, 387
Hpy188III TCNNGA 1 cut(s) 13
Hpy8I GTNNAC 1 cut(s) 178
HpyAV CCTTC 4 cut(s) 29, 166, 196, 326
HpyCH4III ACNGT 2 cut(s) 182, 418
HpyCH4IV ACGT 1 cut(s) 174
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 1 cut(s) 469
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 2 cut(s) 79, 118
KroI GCCGGC 1 cut(s) 146
KroNI GCCGGC 1 cut(s) 148
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 3 cut(s) 117, 367, 439
LpnPI CCDG 9 cut(s) 11, 26, 47, 53, 75, 117, 160, 304, 321
LweI GCATC 2 cut(s) 18, 391
MaeI CTAG 1 cut(s) 479
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 2 cut(s) 151, 412
MalI GATC 3 cut(s) 119, 369, 441
MboI GATC 3 cut(s) 117, 367, 439
MboII GAAGA 4 cut(s) 76, 194, 419, 422
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 4 cut(s) 245, 257, 434, 499
MluNI TGGCCA 1 cut(s) 251
MnlI CCTC 5 cut(s) 9, 37, 218, 268, 293
Mox20I TGGCCA 1 cut(s) 251
MreI CGCCGGCG 1 cut(s) 146
MroNI GCCGGC 1 cut(s) 146
MroXI GAANNNNTTC 1 cut(s) 201
MscI TGGCCA 1 cut(s) 251
MseI TTAA 1 cut(s) 494
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 2 cut(s) 147, 308
MspR9I CCNGG 2 cut(s) 41, 308
MvaI CCWGG 1 cut(s) 41
MwoI GCNNNNNNNGC 1 cut(s) 143
NaeI GCCGGC 1 cut(s) 148
NciI CCSGG 1 cut(s) 308
NdeI CATATG 1 cut(s) 313
NdeII GATC 3 cut(s) 117, 367, 439
NgoMIV GCCGGC 1 cut(s) 146
NlaIII CATG 2 cut(s) 79, 118
NlaIV GGNNCC 2 cut(s) 94, 184
NmeAIII GCCGAG 1 cut(s) 29
NmuCI GTSAC 2 cut(s) 151, 412
NspI RCATGY 1 cut(s) 79
PceI AGGCCT 1 cut(s) 39
PdiI GCCGGC 1 cut(s) 148
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 1 cut(s) 359
Psp6I CCWGG 1 cut(s) 39
PspFI CCCAGC 2 cut(s) 25, 61
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 2 cut(s) 94, 184
PspPI GGNCC 2 cut(s) 92, 182
SaqAI TTAA 1 cut(s) 494
Sau3AI GATC 3 cut(s) 117, 367, 439
Sau96I GGNCC 2 cut(s) 92, 182
ScrFI CCNGG 2 cut(s) 41, 308
SetI ASST 9 cut(s) 27, 48, 126, 158, 177, 243, 272, 323, 341
SfaNI GCATC 2 cut(s) 18, 391
SgrAI CRCCGGYG 1 cut(s) 146
SinI GGWCC 1 cut(s) 182
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
Sse9I AATT 4 cut(s) 245, 257, 434, 499
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 1 cut(s) 168
SspMI CTAG 1 cut(s) 479
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 39, 306
TaaI ACNGT 2 cut(s) 182, 418
TaiI ACGT 1 cut(s) 177
TaqI TCGA 2 cut(s) 298, 408
TasI AATT 4 cut(s) 245, 257, 434, 499
TfiI GAWTC 1 cut(s) 359
Tru1I TTAA 1 cut(s) 494
Tru9I TTAA 1 cut(s) 494
TseFI GTSAC 2 cut(s) 151, 412
Tsp45I GTSAC 2 cut(s) 151, 412
TspDTI ATGAA 1 cut(s) 35
TspGWI ACGGA 1 cut(s) 70
VpaK11BI GGWCC 1 cut(s) 182
XapI RAATTY 1 cut(s) 257
XceI RCATGY 1 cut(s) 79
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.