Rh4AG164500

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003527
Physical Location & Seq
Forward (+)
41624319 .. 41630477
6159 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG164500.1

Sequence Viewer

Length: 369 bp
ATGGCGCCCCGAAGCCCGAAGCCCGAAGCCGACATGATCAAGCTCATCCACCACGCCATCGACTCCGGCGTCACCTTCCTCGACACCGCCGACCTGTACGGTCCCCACACCAACGAAATTCTCCTCGGCAAGGCTTTGAGTGGAGGGGTGAGAGACAAGGTTGAACTGGCCACCAAATGCGGTATCAGGTTTGCGGACAACAAAATGGAGGTTCAAGGCGACCCGGCATATGTGAGAGCTGCTTTTGAAGGAAGCTTGAAACGCCTTGGTGTTGCTTCTGTTGATCTCTATTATCAGATTAGGATTGACACCCGTGTCCCCATTGAAGTCACGCATGAGGAGGATGAAGTTGGGTTCACAAGACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

122

Amino Acids

13.45

Weight (kDa)

5.52

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 6 - 110 3e-22 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 68, 314
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 3 cut(s) 87, 180, 194
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 1 cut(s) 117
AcyI GRCGYC 2 cut(s) 5, 69
AfaI GTAC 1 cut(s) 98
AfiI CCNNNNNNNGG 1 cut(s) 130
AgsI TTSAA 5 cut(s) 164, 215, 248, 259, 326
AluBI AGCT 3 cut(s) 43, 239, 255
AluI AGCT 3 cut(s) 43, 239, 255
Alw26I GTCTC 1 cut(s) 147
AoxI GGCC 1 cut(s) 168
ApeKI GCWGC 1 cut(s) 239
ApoI RAATTY 1 cut(s) 117
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 101
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 2 cut(s) 64, 160
AvaII GGWCC 1 cut(s) 101
BalI TGGCCA 1 cut(s) 170
BanI GGYRCC 1 cut(s) 4
BbvI GCAGC 1 cut(s) 226
BccI CCATC 1 cut(s) 65
BclI TGATCA 1 cut(s) 36
BcnI CCSGG 1 cut(s) 224
BcoDI GTCTC 1 cut(s) 147
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 1 cut(s) 240
BlsI GCNGC 1 cut(s) 241
Bme1390I CCNGG 1 cut(s) 224
Bme18I GGWCC 1 cut(s) 101
BmgT120I GGNCC 1 cut(s) 101
BmiI GGNNCC 2 cut(s) 6, 103
BmrFI CCNGG 1 cut(s) 224
BpuMI CCSGG 1 cut(s) 224
BsaHI GRCGYC 2 cut(s) 5, 69
BsaJI CCNNGG 2 cut(s) 124, 265
Bsc4I CCNNNNNNNGG 1 cut(s) 130
Bse1I ACTGG 1 cut(s) 171
BseDI CCNNGG 2 cut(s) 124, 265
BseGI GGATG 2 cut(s) 45, 349
BseLI CCNNNNNNNGG 1 cut(s) 130
BseNI ACTGG 1 cut(s) 171
BseRI GAGGAG 2 cut(s) 113, 353
BseXI GCAGC 1 cut(s) 226
BshFI GGCC 1 cut(s) 170
BshNI GGYRCC 1 cut(s) 4
BsiSI CCGG 2 cut(s) 66, 224
BslFI GGGAC 2 cut(s) 87, 302
BslI CCNNNNNNNGG 1 cut(s) 130
BsmAI GTCTC 1 cut(s) 147
BsmFI GGGAC 2 cut(s) 87, 302
BsnI GGCC 1 cut(s) 170
Bsp143I GATC 2 cut(s) 36, 283
BspACI CCGC 3 cut(s) 87, 180, 194
BspANI GGCC 1 cut(s) 170
BspLI GGNNCC 2 cut(s) 6, 103
BspT107I GGYRCC 1 cut(s) 4
BsrI ACTGG 1 cut(s) 171
BssECI CCNNGG 2 cut(s) 124, 265
BssMI GATC 2 cut(s) 36, 283
BssNI GRCGYC 2 cut(s) 5, 69
BssT1I CCWWGG 1 cut(s) 265
Bst4CI ACNGT 1 cut(s) 101
BstACI GRCGYC 2 cut(s) 5, 69
BstENI CCTNNNNNAGG 1 cut(s) 128
BstF5I GGATG 2 cut(s) 45, 349
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 2 cut(s) 39, 286
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 2 cut(s) 36, 283
BstMWI GCNNNNNNNGC 1 cut(s) 261
BstSCI CCNGG 1 cut(s) 222
BstV1I GCAGC 1 cut(s) 226
BsuRI GGCC 1 cut(s) 170
BtsCI GGATG 2 cut(s) 45, 349
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 101
CseI GACGC 1 cut(s) 58
Csp6I GTAC 1 cut(s) 97
CviAII CATG 2 cut(s) 34, 335
CviJI RGCY 8 cut(s) 15, 22, 29, 43, 134, 170, 239, 255
CviKI_1 RGCY 8 cut(s) 15, 22, 29, 43, 134, 170, 239, 255
CviQI GTAC 1 cut(s) 97
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 38, 285
DpnII GATC 2 cut(s) 36, 283
DrdI GACNNNNNNGTC 2 cut(s) 68, 314
DseDI GACNNNNNNGTC 2 cut(s) 68, 314
EaeI YGGCCR 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 265
Eco47I GGWCC 1 cut(s) 101
EcoNI CCTNNNNNAGG 1 cut(s) 128
EcoT14I CCWWGG 1 cut(s) 265
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 2 cut(s) 37, 338
FaiI YATR 4 cut(s) 35, 229, 231, 336
FaqI GGGAC 2 cut(s) 87, 302
FatI CATG 2 cut(s) 33, 334
FauNDI CATATG 1 cut(s) 229
FbaI TGATCA 1 cut(s) 36
Fnu4HI GCNGC 1 cut(s) 240
FokI GGATG 2 cut(s) 32, 356
Fsp4HI GCNGC 1 cut(s) 240
GlaI GCGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 240
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 170
HapII CCGG 2 cut(s) 66, 224
HgaI GACGC 1 cut(s) 58
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 2 cut(s) 5, 69
Hin1II CATG 2 cut(s) 37, 338
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HindIII AAGCTT 1 cut(s) 253
HinfI GANTC 1 cut(s) 62
HpaII CCGG 2 cut(s) 66, 224
HphI GGTGA 2 cut(s) 64, 160
Hpy166II GTNNAC 1 cut(s) 357
Hpy188I TCNGA 1 cut(s) 297
Hpy8I GTNNAC 1 cut(s) 357
HpyAV CCTTC 2 cut(s) 85, 242
HpyCH4III ACNGT 1 cut(s) 101
HpyF10VI GCNNNNNNNGC 1 cut(s) 261
Hsp92I GRCGYC 2 cut(s) 5, 69
Hsp92II CATG 2 cut(s) 37, 338
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 2 cut(s) 36, 283
LpnPI CCDG 5 cut(s) 79, 107, 152, 172, 237
Lsp1109I GCAGC 1 cut(s) 226
MaeIII GTNAC 2 cut(s) 70, 328
MalI GATC 2 cut(s) 38, 285
MboI GATC 2 cut(s) 36, 283
MlsI TGGCCA 1 cut(s) 170
MluCI AATT 1 cut(s) 117
MluNI TGGCCA 1 cut(s) 170
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 56
MnlI CCTC 6 cut(s) 89, 134, 137, 202, 331, 334
Mox20I TGGCCA 1 cut(s) 170
MscI TGGCCA 1 cut(s) 170
Msp20I TGGCCA 1 cut(s) 170
MspI CCGG 2 cut(s) 66, 224
MspR9I CCNGG 1 cut(s) 224
MwoI GCNNNNNNNGC 1 cut(s) 261
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 224
NdeI CATATG 1 cut(s) 229
NdeII GATC 2 cut(s) 36, 283
NlaIII CATG 2 cut(s) 37, 338
NlaIV GGNNCC 2 cut(s) 6, 103
NmeAIII GCCGAG 1 cut(s) 105
NmuCI GTSAC 2 cut(s) 70, 328
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 87
PkrI GCNGC 1 cut(s) 241
PleI GAGTC 1 cut(s) 56
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 56
PspN4I GGNNCC 2 cut(s) 6, 103
PspPI GGNCC 1 cut(s) 101
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
SatI GCNGC 1 cut(s) 240
Sau3AI GATC 2 cut(s) 36, 283
Sau96I GGNCC 1 cut(s) 101
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 1 cut(s) 224
SetI ASST 9 cut(s) 45, 77, 96, 162, 191, 213, 241, 257, 367
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 101
Sse9I AATT 1 cut(s) 117
SsiI CCGC 3 cut(s) 87, 180, 194
SspDI GGCGCC 1 cut(s) 4
StyD4I CCNGG 1 cut(s) 222
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 1 cut(s) 101
TaqI TCGA 2 cut(s) 60, 81
TasI AATT 1 cut(s) 117
TseFI GTSAC 2 cut(s) 70, 328
TseI GCWGC 1 cut(s) 239
Tsp45I GTSAC 2 cut(s) 70, 328
TspDTI ATGAA 1 cut(s) 360
VpaK11BI GGWCC 1 cut(s) 101
XagI CCTNNNNNAGG 1 cut(s) 128
XapI RAATTY 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.