Rh4DG160300

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0003527
Physical Location & Seq
Forward (+)
31898236 .. 31901430
3195 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG160300.1

Sequence Viewer

Length: 420 bp
ATGGCGAGCATGAGGAGGATGAAGCTCGGATCACAAGGCCTGGAGGTCTCAGCTCAAGGACTGGGCTGCATGGGCATGTCTGCCTTCTATGGCGCCCCGAAGCCCGAAGCCGACATGATCAAGCTCATCCACCACGCCATCGACTCCGGCGTCACCTTCCTCGACACCGCCGACCTGTACGGTCCCCACACCAACGAAATTCTCCTCGGCAAGGCTTTGAGTGGAGGGGTGAGAGACAAGGTTGAACTGGCCACCAAATGCGGTATCAGGTTTGCGGACAACAAAATGGAGGTTCAAGGCGACCCGGCATATGTGAGAGCTGCTTTTGAAGGAAGCTTGAAACGCCTTGGTGTTGCTTCTGTTGATCTCTATTATCAGATTAGGATTGACACCCGTGTCCCCATTGAAGTCACGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

139

Amino Acids

15.02

Weight (kDa)

6.82

Isoelectric Point (pI)

31.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 137 2.9e-23 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 149, 395
AccB1I GGYRCC 1 cut(s) 92
AciI CCGC 3 cut(s) 168, 261, 275
AclWI GGATC 1 cut(s) 37
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 1 cut(s) 198
AcyI GRCGYC 2 cut(s) 93, 150
AfaI GTAC 1 cut(s) 179
AfiI CCNNNNNNNGG 1 cut(s) 211
AgsI TTSAA 5 cut(s) 245, 296, 329, 340, 407
AjnI CCWGG 1 cut(s) 39
AluBI AGCT 5 cut(s) 25, 53, 124, 320, 336
AluI AGCT 5 cut(s) 25, 53, 124, 320, 336
Alw26I GTCTC 2 cut(s) 52, 228
AlwI GGATC 1 cut(s) 37
AoxI GGCC 2 cut(s) 37, 249
ApeKI GCWGC 2 cut(s) 66, 320
ApoI RAATTY 1 cut(s) 198
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 1 cut(s) 182
AsuC2I CCSGG 1 cut(s) 305
AsuHPI GGTGA 2 cut(s) 145, 241
AvaII GGWCC 1 cut(s) 182
BalI TGGCCA 1 cut(s) 251
BanI GGYRCC 1 cut(s) 92
BbvI GCAGC 2 cut(s) 53, 307
BccI CCATC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 41
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 1 cut(s) 305
BcoDI GTCTC 2 cut(s) 52, 228
BfoI RGCGCY 1 cut(s) 96
BisI GCNGC 2 cut(s) 67, 321
BlsI GCNGC 2 cut(s) 68, 322
Bme1390I CCNGG 2 cut(s) 41, 305
Bme18I GGWCC 1 cut(s) 182
BmgT120I GGNCC 1 cut(s) 182
BmiI GGNNCC 2 cut(s) 94, 184
BmrFI CCNGG 2 cut(s) 41, 305
BmrI ACTGGG 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 71
BpmI CTGGAG 1 cut(s) 62
BpuEI CTTGAG 1 cut(s) 39
BpuMI CCSGG 1 cut(s) 305
BsaHI GRCGYC 2 cut(s) 93, 150
BsaI GGTCTC 1 cut(s) 52
BsaJI CCNNGG 2 cut(s) 205, 346
Bsc4I CCNNNNNNNGG 1 cut(s) 211
Bse1I ACTGG 2 cut(s) 66, 252
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 2 cut(s) 205, 346
BseGI GGATG 2 cut(s) 24, 126
BseLI CCNNNNNNNGG 1 cut(s) 211
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 2 cut(s) 66, 252
BseRI GAGGAG 2 cut(s) 28, 194
BseXI GCAGC 2 cut(s) 53, 307
BshFI GGCC 2 cut(s) 39, 251
BshNI GGYRCC 1 cut(s) 92
BsiSI CCGG 2 cut(s) 147, 305
BslFI GGGAC 2 cut(s) 168, 383
BslI CCNNNNNNNGG 1 cut(s) 211
BsmAI GTCTC 2 cut(s) 52, 228
BsmFI GGGAC 2 cut(s) 168, 383
BsnI GGCC 2 cut(s) 39, 251
Bso31I GGTCTC 1 cut(s) 52
Bsp143I GATC 3 cut(s) 29, 117, 364
BspACI CCGC 3 cut(s) 168, 261, 275
BspANI GGCC 2 cut(s) 39, 251
BspCNI CTCAG 1 cut(s) 62
BspLI GGNNCC 2 cut(s) 94, 184
BspPI GGATC 1 cut(s) 37
BspT107I GGYRCC 1 cut(s) 92
BspTNI GGTCTC 1 cut(s) 52
BsrI ACTGG 2 cut(s) 66, 252
BssECI CCNNGG 2 cut(s) 205, 346
BssMI GATC 3 cut(s) 29, 117, 364
BssNI GRCGYC 2 cut(s) 93, 150
BssT1I CCWWGG 1 cut(s) 346
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 2 cut(s) 182, 415
BstACI GRCGYC 2 cut(s) 93, 150
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 49
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 2 cut(s) 24, 126
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 3 cut(s) 32, 120, 367
BstMAI GTCTC 2 cut(s) 52, 228
BstMBI GATC 3 cut(s) 29, 117, 364
BstMWI GCNNNNNNNGC 2 cut(s) 72, 342
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 1 cut(s) 79
BstSCI CCNGG 2 cut(s) 39, 303
BstV1I GCAGC 2 cut(s) 53, 307
BsuRI GGCC 2 cut(s) 39, 251
BtsCI GGATG 2 cut(s) 24, 126
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 182
CseI GACGC 1 cut(s) 139
Csp6I GTAC 1 cut(s) 178
CviAII CATG 4 cut(s) 10, 70, 76, 115
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 1 cut(s) 49
DinI GGCGCC 1 cut(s) 94
DpnI GATC 3 cut(s) 31, 119, 366
DpnII GATC 3 cut(s) 29, 117, 364
DrdI GACNNNNNNGTC 2 cut(s) 149, 395
DseDI GACNNNNNNGTC 2 cut(s) 149, 395
EaeI YGGCCR 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 346
Eco147I AGGCCT 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 182
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoRII CCWGG 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 346
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 346
FaeI CATG 4 cut(s) 13, 73, 79, 118
FaiI YATR 7 cut(s) 11, 71, 77, 90, 116, 310, 312
FaqI GGGAC 2 cut(s) 168, 383
FatI CATG 4 cut(s) 9, 69, 75, 114
FauNDI CATATG 1 cut(s) 310
FbaI TGATCA 1 cut(s) 117
Fnu4HI GCNGC 2 cut(s) 67, 321
FokI GGATG 2 cut(s) 31, 113
Fsp4HI GCNGC 2 cut(s) 67, 321
GlaI GCGC 1 cut(s) 94
GluI GCNGC 2 cut(s) 67, 321
GsuI CTGGAG 1 cut(s) 62
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 2 cut(s) 39, 251
HapII CCGG 2 cut(s) 147, 305
HgaI GACGC 1 cut(s) 139
HhaI GCGC 1 cut(s) 95
Hin1I GRCGYC 2 cut(s) 93, 150
Hin1II CATG 4 cut(s) 13, 73, 79, 118
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HindIII AAGCTT 1 cut(s) 334
HinfI GANTC 1 cut(s) 143
HpaII CCGG 2 cut(s) 147, 305
HphI GGTGA 2 cut(s) 145, 241
Hpy188I TCNGA 2 cut(s) 29, 378
HpyAV CCTTC 3 cut(s) 94, 166, 323
HpyCH4III ACNGT 2 cut(s) 182, 415
HpyCH4V TGCA 1 cut(s) 69
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 342
HpyF3I CTNAG 1 cut(s) 49
Hsp92I GRCGYC 2 cut(s) 93, 150
Hsp92II CATG 4 cut(s) 13, 73, 79, 118
HspAI GCGC 1 cut(s) 93
KasI GGCGCC 1 cut(s) 92
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 3 cut(s) 29, 117, 364
LpnPI CCDG 8 cut(s) 26, 47, 53, 160, 188, 233, 253, 318
Lsp1109I GCAGC 2 cut(s) 53, 307
MaeIII GTNAC 2 cut(s) 151, 409
MalI GATC 3 cut(s) 31, 119, 366
MboI GATC 3 cut(s) 29, 117, 364
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 1 cut(s) 198
MluNI TGGCCA 1 cut(s) 251
Mly113I GGCGCC 1 cut(s) 93
MlyI GAGTC 1 cut(s) 137
MnlI CCTC 7 cut(s) 6, 9, 37, 170, 215, 218, 283
Mox20I TGGCCA 1 cut(s) 251
MscI TGGCCA 1 cut(s) 251
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 251
MspI CCGG 2 cut(s) 147, 305
MspR9I CCNGG 2 cut(s) 41, 305
MvaI CCWGG 1 cut(s) 41
MwoI GCNNNNNNNGC 2 cut(s) 72, 342
NarI GGCGCC 1 cut(s) 93
NciI CCSGG 1 cut(s) 305
NdeI CATATG 1 cut(s) 310
NdeII GATC 3 cut(s) 29, 117, 364
NlaIII CATG 4 cut(s) 13, 73, 79, 118
NlaIV GGNNCC 2 cut(s) 94, 184
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 2 cut(s) 151, 409
NspI RCATGY 1 cut(s) 79
PceI AGGCCT 1 cut(s) 39
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 168
PkrI GCNGC 2 cut(s) 68, 322
PleI GAGTC 1 cut(s) 137
PluTI GGCGCC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 137
Psp6I CCWGG 1 cut(s) 39
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 2 cut(s) 94, 184
PspPI GGNCC 1 cut(s) 182
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
RseI CAYNNNNRTG 1 cut(s) 74
SatI GCNGC 2 cut(s) 67, 321
Sau3AI GATC 3 cut(s) 29, 117, 364
Sau96I GGNCC 1 cut(s) 182
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 2 cut(s) 41, 305
SfoI GGCGCC 1 cut(s) 94
SinI GGWCC 1 cut(s) 182
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
Sse9I AATT 1 cut(s) 198
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 3 cut(s) 168, 261, 275
SspDI GGCGCC 1 cut(s) 92
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 39, 303
StyI CCWWGG 1 cut(s) 346
TaaI ACNGT 2 cut(s) 182, 415
TaqI TCGA 2 cut(s) 141, 162
TasI AATT 1 cut(s) 198
TseFI GTSAC 2 cut(s) 151, 409
TseI GCWGC 2 cut(s) 66, 320
Tsp45I GTSAC 2 cut(s) 151, 409
TspDTI ATGAA 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 182
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 1 cut(s) 198
XceI RCATGY 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.