RchiOBHm_Chr3g0473471

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001542
Physical Location & Seq
Reverse (-)
19306102 .. 19307914
1813 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1740 bp
ATGGTAACTGGGTTTTCTCAGAACTTGAGGTTCTCCGAGAGTTTCAAGGCCTTTTCGCAGATGAGAATTGCTGGGGAGAGCCCGACCCAGTTTGCATTTGCAAGTGTCATCAGAGCTTGTGTGGTTCTTGGGTCGGTTGAGATTGGGAGGCAGCTGCATTCTCTTGCTTTGAAATTGGGCTTGGCTTGTGAGTTGTTTGTGGGCAGTAACTTGGCGGATATGTATTCGAAATGTGGGTTCATGGTTGAGGCTTGTAAGTTTTTCGAGGAAATGCCGAGTAAGGATGCTGTGTCTTGGACTTCGATGATTGATGGGTATGCAAAAAGTGGGGATTTTGAGGCAGCTTTACTGAGTTATCAGAGGATGATCAATGATGGGATTGGTGTAGATAAATATGTTGTTTCTAGTGCATTGAGTGCTTGTTCTGCACTGAAGGCTTGTCAGTTTGGAAAGTGTGTTCATTCGACGGTTGTGAAGTTAGGATTGGAAGTAGAGGTTGCTGTGGGAAATGCTCTGGTTGATATGTACTCGAAATCAGGAGATATGGAGAGTGCTTTAAATGTGTTTCATGTTCACCCTGAGTGTAGAAATATTGTGTCGTATTCCTCTCTGATCAATGGTTATGTCGAGATGGATGAAATCGAGAAGGCTTTTAGTGTGTTTGTTGACCTGCAGAGGGGGGGGGGAGTTGAGCCTAATCAGTTCACATTCTCTAGCTTGGTCAAGGCCTGTGCCAACCAAGCTGCACTTGAACAAGGGATTCAACTTCATGCTCAGGTGATCAAGTTTAATTTTGATAGAGACAATTTTGTGTCTTCTGTTCTTGTTGATATGTATGGAAAATGTGGGTTACTTGATCACTCAATACAAGTGTTTGACGAGATAGCAAACCCGACTGAAGTTGCATGGAATTCGTTGCCAAGTGTATTTGCAGTTAATGGCTTAGGAAATGACGCCTTGAAAACTTTTAGTAGAATGGTCCGGGCAGGAGTGAAACCAAATGCAATAACATTTATCATCCTTTTAACAGGTTGTAGCCATTCTGGATCGGTAGAGGAAGGTTTAACGTACTTTTACTTTATGGAGAAAACATATGGCATGGTACCTAGAGCTGGACATTACTCTTGCGTTATTGATTTACTTGGCCGGGCTGGAAGGCTTGAAGAGGCCGAAGAATTCATTAACAGCATGCCAATGCAGCCAAATGCTTTTGGATGGTGCTTTTTTCTTGGCGCTTGCACAATTCGTGGTGATAAAGAGGGGCAAACTGGCAGCAGAAAATGGGAAGATTTCAGGGGTGTGAGGAAGATAATGAGGGATGGCAGCATGAAGAAATTGCCCGGTTATAGTTGGGTTGACATTGGAAACAAAACACACACGTTTGGAGCCAAAGATTGGTCTCATCCTCAGAAGAAAAAAATATATGAGAAACTTGATAGTCTTCTTGATCAGATCAAGAAAGCTGGATATGTTCCAAATACAGATCTTGTTCTACATGATATGGACGACAATTCAAAGGTAGAGCTTCTTCACCATCATAGTGAGAGGATAGCGATTGCATTTGCACTGGTAAGTATGCCTGGGAAGCCAATCATTGTGAAGAAAAATATAAGGGTGTGCTTGGATTGCCATTCTGCAATCAAGTACATCTCCTTGGTTGTTGGAAGAAAGATCATAGTTAGGGACAATACTCGGTTCCACCATTTCGCAGATGGGTTGTGTTCATGTGGAGATTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

579

Amino Acids

64.02

Weight (kDa)

6.63

Isoelectric Point (pI)

29.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 94 - 141 2e-06 PPR repeat family
PPR PF01535 97 - 127 4.4e-07 PPR repeat
PPR_long PF17177 103 - 247 8.9e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 196 - 246 9.2e-12 PPR repeat family
PPR_2 PF13041 298 - 346 8e-10 PPR repeat family
Eplus_motif PF20430 458 - 485 1.8e-08 E+ motif
DYW_deaminase PF14432 489 - 579 5.4e-36 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 678
Acc65I GGTACC 1 cut(s) 1102
AccB1I GGYRCC 1 cut(s) 1102
AccB7I CCANNNNNTGG 1 cut(s) 1395
AciI CCGC 1 cut(s) 215
AclWI GGATC 1 cut(s) 1054
AcoI YGGCCR 1 cut(s) 1144
AcsI RAATTY 2 cut(s) 910, 1175
AcuI CTGAAG 2 cut(s) 452, 918
AcyI GRCGYC 1 cut(s) 954
AfaI GTAC 4 cut(s) 527, 1070, 1104, 1646
AfiI CCNNNNNNNGG 3 cut(s) 676, 1112, 1395
AflIII ACRYGT 1 cut(s) 1377
AgsI TTSAA 7 cut(s) 46, 172, 752, 764, 961, 1163, 1515
AjnI CCWGG 1 cut(s) 1579
AjuI GAANNNNNNNTTGG 4 cut(s) 164, 196, 467, 499
AloI GAACNNNNNNTCC 2 cut(s) 441, 473
AluBI AGCT 8 cut(s) 116, 154, 344, 717, 743, 1112, 1463, 1525
AluI AGCT 8 cut(s) 116, 154, 344, 717, 743, 1112, 1463, 1525
Alw26I GTCTC 2 cut(s) 795, 1404
AlwI GGATC 1 cut(s) 1054
AoxI GGCC 4 cut(s) 48, 726, 1144, 1167
ApeKI GCWGC 7 cut(s) 151, 154, 341, 743, 1198, 1272, 1323
ApoI RAATTY 2 cut(s) 910, 1175
Asp718I GGTACC 1 cut(s) 1102
AspLEI GCGC 1 cut(s) 1235
AspS9I GGNCC 1 cut(s) 979
AsuC2I CCSGG 3 cut(s) 983, 1148, 1341
AsuHPI GGTGA 4 cut(s) 566, 790, 1262, 1523
AsuII TTCGAA 1 cut(s) 227
AvaII GGWCC 1 cut(s) 979
BanI GGYRCC 1 cut(s) 1102
BanII GRGCYC 1 cut(s) 83
BarI GAAGNNNNNNTAC 2 cut(s) 1512, 1544
BbsI GAAGAC 2 cut(s) 807, 1433
BbvI GCAGC 7 cut(s) 141, 163, 353, 730, 1210, 1284, 1335
BccI CCATC 7 cut(s) 305, 368, 625, 1209, 1313, 1542, 1706
BcgI CGANNNNNNTGC 2 cut(s) 894, 928
BciT130I CCWGG 1 cut(s) 1581
BclI TGATCA 5 cut(s) 366, 612, 780, 856, 1447
BcnI CCSGG 3 cut(s) 983, 1148, 1341
BcoDI GTCTC 2 cut(s) 795, 1404
BfaI CTAG 4 cut(s) 405, 714, 1107, 1738
BfmI CTRYAG 1 cut(s) 671
BfoI RGCGCY 1 cut(s) 1236
BfuAI ACCTGC 1 cut(s) 678
BglII AGATCT 1 cut(s) 1483
BisI GCNGC 7 cut(s) 152, 155, 342, 744, 1199, 1273, 1324
BlsI GCNGC 7 cut(s) 153, 156, 343, 745, 1200, 1274, 1325
Bme1390I CCNGG 4 cut(s) 983, 1148, 1341, 1581
Bme18I GGWCC 1 cut(s) 979
BmgT120I GGNCC 1 cut(s) 979
BmiI GGNNCC 3 cut(s) 1104, 1387, 1697
BmrFI CCNGG 4 cut(s) 983, 1148, 1341, 1581
BmrI ACTGGG 2 cut(s) 18, 82
BmsI GCATC 1 cut(s) 274
BmuI ACTGGG 2 cut(s) 18, 82
BpiI GAAGAC 2 cut(s) 807, 1433
Bpu10I CCTNAGC 2 cut(s) 774, 943
Bpu14I TTCGAA 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 46
BpuMI CCSGG 3 cut(s) 983, 1148, 1341
BsaHI GRCGYC 1 cut(s) 954
BsaI GGTCTC 1 cut(s) 1404
BsaJI CCNNGG 2 cut(s) 1580, 1653
Bsc4I CCNNNNNNNGG 3 cut(s) 676, 1112, 1395
Bse1I ACTGG 4 cut(s) 13, 88, 1273, 1572
BseBI CCWGG 1 cut(s) 1581
BseDI CCNNGG 2 cut(s) 1580, 1653
BseGI GGATG 7 cut(s) 289, 369, 640, 1017, 1220, 1324, 1402
BseLI CCNNNNNNNGG 3 cut(s) 676, 1112, 1395
BseMII CTCAG 5 cut(s) 32, 341, 570, 788, 1421
BseNI ACTGG 4 cut(s) 13, 88, 1273, 1572
BseXI GCAGC 7 cut(s) 141, 163, 353, 730, 1210, 1284, 1335
BseYI CCCAGC 1 cut(s) 71
BsgI GTGCAG 2 cut(s) 411, 729
BshFI GGCC 4 cut(s) 50, 728, 1146, 1169
BshNI GGYRCC 1 cut(s) 1102
BsiSI CCGG 3 cut(s) 982, 1147, 1341
BslFI GGGAC 1 cut(s) 1697
BslI CCNNNNNNNGG 3 cut(s) 676, 1112, 1395
BsmAI GTCTC 2 cut(s) 795, 1404
BsmFI GGGAC 1 cut(s) 1697
BsmI GAATGC 1 cut(s) 157
BsnI GGCC 4 cut(s) 50, 728, 1146, 1169
Bso31I GGTCTC 1 cut(s) 1404
Bsp119I TTCGAA 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 83
Bsp143I GATC 9 cut(s) 366, 612, 780, 856, 1046, 1447, 1452, 1483, 1671
BspACI CCGC 1 cut(s) 215
BspANI GGCC 4 cut(s) 50, 728, 1146, 1169
BspCNI CTCAG 5 cut(s) 31, 342, 571, 787, 1420
BspLI GGNNCC 3 cut(s) 1104, 1387, 1697
BspMAI CTGCAG 1 cut(s) 675
BspMI ACCTGC 1 cut(s) 678
BspPI GGATC 1 cut(s) 1054
BspT104I TTCGAA 1 cut(s) 227
BspT107I GGYRCC 1 cut(s) 1102
BspTNI GGTCTC 1 cut(s) 1404
BsrI ACTGG 4 cut(s) 13, 88, 1273, 1572
BssECI CCNNGG 2 cut(s) 1580, 1653
BssMI GATC 9 cut(s) 366, 612, 780, 856, 1046, 1447, 1452, 1483, 1671
BssNI GRCGYC 1 cut(s) 954
BssT1I CCWWGG 1 cut(s) 1653
Bst2UI CCWGG 1 cut(s) 1581
Bst4CI ACNGT 1 cut(s) 469
Bst6I CTCTTC 1 cut(s) 1158
BstACI GRCGYC 1 cut(s) 954
BstAPI GCANNNNNTGC 1 cut(s) 416
BstBI TTCGAA 1 cut(s) 227
BstC8I GCNNGC 2 cut(s) 1190, 1237
BstDEI CTNAG 6 cut(s) 18, 350, 579, 774, 943, 1407
BstF5I GGATG 7 cut(s) 289, 369, 640, 1017, 1220, 1324, 1402
BstH2I RGCGCY 1 cut(s) 1236
BstHHI GCGC 1 cut(s) 1235
BstKTI GATC 9 cut(s) 369, 615, 783, 859, 1049, 1450, 1455, 1486, 1674
BstMAI GTCTC 2 cut(s) 795, 1404
BstMBI GATC 9 cut(s) 366, 612, 780, 856, 1046, 1447, 1452, 1483, 1671
BstMWI GCNNNNNNNGC 7 cut(s) 416, 425, 434, 740, 1198, 1585, 1626
BstNI CCWGG 1 cut(s) 1581
BstNSI RCATGY 1 cut(s) 1192
BstSCI CCNGG 4 cut(s) 981, 1146, 1339, 1579
BstSFI CTRYAG 1 cut(s) 671
BstV1I GCAGC 7 cut(s) 141, 163, 353, 730, 1210, 1284, 1335
BstV2I GAAGAC 2 cut(s) 807, 1433
BstX2I RGATCY 1 cut(s) 1483
BstYI RGATCY 1 cut(s) 1483
BsuRI GGCC 4 cut(s) 50, 728, 1146, 1169
BtsCI GGATG 7 cut(s) 289, 369, 640, 1017, 1220, 1324, 1402
BtsIMutI CAGTG 2 cut(s) 428, 1565
BveI ACCTGC 1 cut(s) 678
Cac8I GCNNGC 2 cut(s) 1190, 1237
CfoI GCGC 1 cut(s) 1235
Cfr13I GGNCC 1 cut(s) 979
CseI GACGC 1 cut(s) 962
Csp6I GTAC 4 cut(s) 526, 1069, 1103, 1645
CviAII CATG 9 cut(s) 241, 569, 770, 906, 1099, 1189, 1327, 1496, 1725
CviQI GTAC 4 cut(s) 526, 1069, 1103, 1645
DdeI CTNAG 6 cut(s) 18, 350, 579, 774, 943, 1407
DpnI GATC 9 cut(s) 368, 614, 782, 858, 1048, 1449, 1454, 1485, 1673
DpnII GATC 9 cut(s) 366, 612, 780, 856, 1046, 1447, 1452, 1483, 1671
DraI TTTAAA 1 cut(s) 558
EaeI YGGCCR 1 cut(s) 1144
Eam1104I CTCTTC 1 cut(s) 1158
EarI CTCTTC 1 cut(s) 1158
EciI GGCGGA 1 cut(s) 230
Eco130I CCWWGG 1 cut(s) 1653
Eco147I AGGCCT 2 cut(s) 50, 728
Eco24I GRGCYC 1 cut(s) 83
Eco31I GGTCTC 1 cut(s) 1404
Eco47I GGWCC 1 cut(s) 979
Eco57I CTGAAG 2 cut(s) 452, 918
EcoRI GAATTC 2 cut(s) 910, 1175
EcoRII CCWGG 1 cut(s) 1579
EcoT14I CCWWGG 1 cut(s) 1653
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 1653
FaeI CATG 9 cut(s) 244, 572, 773, 909, 1102, 1192, 1330, 1499, 1728
FalI AAGNNNNNCTT 2 cut(s) 732, 764
FaqI GGGAC 1 cut(s) 1697
FatI CATG 9 cut(s) 240, 568, 769, 905, 1098, 1188, 1326, 1495, 1724
FauNDI CATATG 1 cut(s) 1093
FbaI TGATCA 5 cut(s) 366, 612, 780, 856, 1447
Fnu4HI GCNGC 7 cut(s) 152, 155, 342, 744, 1199, 1273, 1324
FokI GGATG 7 cut(s) 296, 376, 647, 1004, 1227, 1331, 1389
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 7 cut(s) 152, 155, 342, 744, 1199, 1273, 1324
FspBI CTAG 4 cut(s) 405, 714, 1107, 1738
GlaI GCGC 1 cut(s) 1234
GluI GCNGC 7 cut(s) 152, 155, 342, 744, 1199, 1273, 1324
GsaI CCCAGC 1 cut(s) 75
HaeII RGCGCY 1 cut(s) 1236
HaeIII GGCC 4 cut(s) 50, 728, 1146, 1169
HapII CCGG 3 cut(s) 982, 1147, 1341
HgaI GACGC 1 cut(s) 962
HhaI GCGC 1 cut(s) 1235
Hin1I GRCGYC 1 cut(s) 954
Hin1II CATG 9 cut(s) 244, 572, 773, 909, 1102, 1192, 1330, 1499, 1728
Hin6I GCGC 1 cut(s) 1233
HinP1I GCGC 1 cut(s) 1233
HincII GTYRAC 2 cut(s) 667, 1357
HindII GTYRAC 2 cut(s) 667, 1357
HinfI GANTC 1 cut(s) 760
HpaII CCGG 3 cut(s) 982, 1147, 1341
HphI GGTGA 4 cut(s) 566, 790, 1262, 1523
Hpy166II GTNNAC 4 cut(s) 574, 667, 705, 1357
Hpy188I TCNGA 7 cut(s) 21, 37, 113, 360, 612, 1410, 1452
Hpy188III TCNNGA 6 cut(s) 537, 628, 643, 1044, 1445, 1456
Hpy8I GTNNAC 4 cut(s) 574, 667, 705, 1357
Hpy99I CGWCG 1 cut(s) 469
HpyAV CCTTC 4 cut(s) 427, 640, 1052, 1149
HpyCH4III ACNGT 1 cut(s) 469
HpyCH4IV ACGT 2 cut(s) 1067, 1379
HpyF10VI GCNNNNNNNGC 7 cut(s) 416, 425, 434, 740, 1198, 1585, 1626
HpyF3I CTNAG 6 cut(s) 18, 350, 579, 774, 943, 1407
HpySE526I ACGT 2 cut(s) 1067, 1379
Hsp92I GRCGYC 1 cut(s) 954
Hsp92II CATG 9 cut(s) 244, 572, 773, 909, 1102, 1192, 1330, 1499, 1728
HspAI GCGC 1 cut(s) 1233
KpnI GGTACC 1 cut(s) 1106
Ksp22I TGATCA 5 cut(s) 366, 612, 780, 856, 1447
Kzo9I GATC 9 cut(s) 366, 612, 780, 856, 1046, 1447, 1452, 1483, 1671
LmnI GCTCC 1 cut(s) 1385
Lsp1109I GCAGC 7 cut(s) 141, 163, 353, 730, 1210, 1284, 1335
LweI GCATC 1 cut(s) 274
MaeI CTAG 4 cut(s) 405, 714, 1107, 1738
MaeII ACGT 2 cut(s) 1067, 1379
MaeIII GTNAC 3 cut(s) 4, 206, 849
MalI GATC 9 cut(s) 368, 614, 782, 858, 1048, 1449, 1454, 1485, 1673
MboI GATC 9 cut(s) 366, 612, 780, 856, 1046, 1447, 1452, 1483, 1671
MflI RGATCY 1 cut(s) 1483
MhlI GDGCHC 1 cut(s) 83
MluCI AATT 9 cut(s) 66, 173, 790, 805, 910, 1175, 1242, 1334, 1510
MmeI TCCRAC 1 cut(s) 1642
MseI TTAA 6 cut(s) 557, 789, 936, 1025, 1064, 1182
MslI CAYNNNNRTG 2 cut(s) 1193, 1539
MspA1I CMGCKG 1 cut(s) 154
MspI CCGG 3 cut(s) 982, 1147, 1341
MspR9I CCNGG 4 cut(s) 983, 1148, 1341, 1581
Mva1269I GAATGC 1 cut(s) 157
MvaI CCWGG 1 cut(s) 1581
MwoI GCNNNNNNNGC 7 cut(s) 416, 425, 434, 740, 1198, 1585, 1626
NciI CCSGG 3 cut(s) 983, 1148, 1341
NdeI CATATG 1 cut(s) 1093
NdeII GATC 9 cut(s) 366, 612, 780, 856, 1046, 1447, 1452, 1483, 1671
NlaIII CATG 9 cut(s) 244, 572, 773, 909, 1102, 1192, 1330, 1499, 1728
NlaIV GGNNCC 3 cut(s) 1104, 1387, 1697
NmeAIII GCCGAG 1 cut(s) 300
NspI RCATGY 1 cut(s) 1192
NspV TTCGAA 1 cut(s) 227
PaeI GCATGC 1 cut(s) 1192
PceI AGGCCT 2 cut(s) 50, 728
PctI GAATGC 1 cut(s) 157
PfeI GAWTC 1 cut(s) 760
PflMI CCANNNNNTGG 1 cut(s) 1395
PkrI GCNGC 7 cut(s) 153, 156, 343, 745, 1200, 1274, 1325
Psp6I CCWGG 1 cut(s) 1579
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 1 cut(s) 1579
PspN4I GGNNCC 3 cut(s) 1104, 1387, 1697
PspPI GGNCC 1 cut(s) 979
PstI CTGCAG 1 cut(s) 675
PsuI RGATCY 1 cut(s) 1483
PvuII CAGCTG 1 cut(s) 154
RsaI GTAC 4 cut(s) 527, 1070, 1104, 1646
RsaNI GTAC 4 cut(s) 526, 1069, 1103, 1645
RseI CAYNNNNRTG 2 cut(s) 1193, 1539
SaqAI TTAA 6 cut(s) 557, 789, 936, 1025, 1064, 1182
SatI GCNGC 7 cut(s) 152, 155, 342, 744, 1199, 1273, 1324
Sau3AI GATC 9 cut(s) 366, 612, 780, 856, 1046, 1447, 1452, 1483, 1671
Sau96I GGNCC 1 cut(s) 979
ScrFI CCNGG 4 cut(s) 983, 1148, 1341, 1581
SduI GDGCHC 1 cut(s) 83
SfaNI GCATC 1 cut(s) 274
SfcI CTRYAG 1 cut(s) 671
SfuI TTCGAA 1 cut(s) 227
SinI GGWCC 1 cut(s) 979
SmiMI CAYNNNNRTG 2 cut(s) 1193, 1539
SmlI CTYRAG 1 cut(s) 25
SmoI CTYRAG 1 cut(s) 25
SphI GCATGC 1 cut(s) 1192
Sse9I AATT 9 cut(s) 66, 173, 790, 805, 910, 1175, 1242, 1334, 1510
SseBI AGGCCT 2 cut(s) 50, 728
SsiI CCGC 1 cut(s) 215
SspI AATATT 1 cut(s) 592
SspMI CTAG 4 cut(s) 405, 714, 1107, 1738
StuI AGGCCT 2 cut(s) 50, 728
StyD4I CCNGG 4 cut(s) 981, 1146, 1339, 1579
StyI CCWWGG 1 cut(s) 1653
TaaI ACNGT 1 cut(s) 469
TaiI ACGT 2 cut(s) 1070, 1382
TaqI TCGA 7 cut(s) 227, 264, 302, 464, 530, 627, 642
TasI AATT 9 cut(s) 66, 173, 790, 805, 910, 1175, 1242, 1334, 1510
TatI WGTACW 2 cut(s) 525, 1644
TfiI GAWTC 1 cut(s) 760
Tru1I TTAA 6 cut(s) 557, 789, 936, 1025, 1064, 1182
Tru9I TTAA 6 cut(s) 557, 789, 936, 1025, 1064, 1182
TscAI CASTG 2 cut(s) 435, 1572
TseI GCWGC 7 cut(s) 151, 154, 341, 743, 1198, 1272, 1323
TspDTI ATGAA 8 cut(s) 229, 449, 557, 651, 758, 1168, 1343, 1713
TspRI CASTG 2 cut(s) 435, 1572
Van91I CCANNNNNTGG 1 cut(s) 1395
VpaK11BI GGWCC 1 cut(s) 979
XapI RAATTY 2 cut(s) 910, 1175
XceI RCATGY 1 cut(s) 1192
XcmI CCANNNNNNNNNTGG 1 cut(s) 1709
XspI CTAG 4 cut(s) 405, 714, 1107, 1738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.