RchiOBHm_Chr2g0110621

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002881
Physical Location & Seq
Forward (+)
22163626 .. 22165218
1593 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 951 bp
ATGTCACTTGATTGCTGTTTGCTGTTTGCTGGGAAAAAAAATAAATTGTATGATCATTTTTGTAGGCATGAAATTGTTGGCCAAGTGACCAAGGTTGGGCTCAACGTCAAAAAATTCAAAGTTGGAGATGTAGCCGGAGTAGGGTGCATGATGGGTTCATGTGGCTCATGCGAGAACTGCAAACAAGACTTGGAAAATTACTGCCCCAAAATGTTATGGACCTTCAGTGGACAATATGAAGATGGAACAAGAGCCTTTGGTGGATATTCCAATAAGTTTGTTGTGGAAGAGCACTTTGCCGTCCGAATCCCAAATGACATACCACTAGCAGGTGTCGCCCCGCTATTGTGTGCTGGGATTACCGTGTATAGCCCCCTGAAATATTTCGGACTAGTCCCCCAGCCCGGTAGCAAGAAACATTTGGGTGTTGTTGGGCTTGGAGGTGTAGGTCATATGGCCGTGAAGTTTGCCAAAGCTTTCGGAGCAAAGGTCACTGTCATTAGTTCCTCTCCGAGTAAGGAAAAGGAAGCAGTGGAACAACTTGGTGCAGATTCATTTTTGATCAGTCATGACCATGAGAAATTGGAGGCTGCCTTGGGCACCATGGATGGTATCATCGACACTGTTTCTGCTTCTCACTCTCTCCTCCCATTGATTGGCTTGCTAAAAACTAGTGGAAAACTAGTTTTGGTGGGTGCTCCAGTGGAGCCACCTGAGCTTCCCGTCTTCCCTTTAATTATGGGGAGGAAAGTTATTGCTGGTAGTGCAATTGGGGGGATGAAAGAGACTCAAGAGATGATAGATTTTGCAGCAAAGCACAACATCATAGCAGATGTCGAAGTTATTCCAATGGATTATGTGAACACTGCTCTTGAACGACTTGAGAAAGGCGACAATGTTAAATATCGCTTTGTCATCGATGTTGCCAACACAATAAAATGTGCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

316

Amino Acids

33.91

Weight (kDa)

7.08

Isoelectric Point (pI)

27.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 17 - 104 3.9e-13 Alcohol dehydrogenase GroES-like domain
2-Hacid_dh_C PF02826 137 - 181 4.9e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
AlaDh_PNT_C PF01262 141 - 211 1.4e-06 Alanine dehydrogenase/PNT, C-terminal domain
ADH_zinc_N PF00107 148 - 271 6e-23 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 320
Acc36I ACCTGC 1 cut(s) 320
AccB1I GGYRCC 1 cut(s) 599
AccB7I CCANNNNNTGG 1 cut(s) 656
AciI CCGC 1 cut(s) 341
AcoI YGGCCR 2 cut(s) 79, 456
AcsI RAATTY 1 cut(s) 113
AcuI CTGAAG 1 cut(s) 208
AfiI CCNNNNNNNGG 5 cut(s) 96, 141, 329, 404, 656
AgsI TTSAA 2 cut(s) 118, 875
AhlI ACTAGT 3 cut(s) 391, 671, 682
AluBI AGCT 2 cut(s) 476, 718
AluI AGCT 2 cut(s) 476, 718
Alw21I GWGCWC 2 cut(s) 294, 700
Alw26I GTCTC 1 cut(s) 779
AoxI GGCC 2 cut(s) 79, 456
ApeKI GCWGC 2 cut(s) 590, 809
ApoI RAATTY 1 cut(s) 113
Asp700I GAANNNNTTC 2 cut(s) 383, 843
AspS9I GGNCC 1 cut(s) 219
AsuC2I CCSGG 1 cut(s) 405
AvaII GGWCC 1 cut(s) 219
BaeGI GKGCMC 1 cut(s) 602
BalI TGGCCA 1 cut(s) 81
BanI GGYRCC 1 cut(s) 599
BanII GRGCYC 1 cut(s) 102
BbsI GAAGAC 1 cut(s) 718
Bbv12I GWGCWC 2 cut(s) 294, 700
BbvI GCAGC 2 cut(s) 577, 821
BccI CCATC 3 cut(s) 145, 236, 602
BceAI ACGGC 2 cut(s) 284, 443
BclI TGATCA 2 cut(s) 52, 561
BcnI CCSGG 1 cut(s) 405
BcoDI GTCTC 1 cut(s) 779
BcuI ACTAGT 3 cut(s) 391, 671, 682
BfaI CTAG 5 cut(s) 326, 392, 672, 683, 949
BfuAI ACCTGC 1 cut(s) 320
BisI GCNGC 2 cut(s) 591, 810
BlsI GCNGC 2 cut(s) 592, 811
Bme1390I CCNGG 1 cut(s) 405
Bme18I GGWCC 1 cut(s) 219
BmgT120I GGNCC 1 cut(s) 219
BmiI GGNNCC 2 cut(s) 601, 708
BmrFI CCNGG 1 cut(s) 405
BpiI GAAGAC 1 cut(s) 718
BpmI CTGGAG 1 cut(s) 684
Bpu10I CCTNAGC 1 cut(s) 714
BpuEI CTTGAG 2 cut(s) 774, 902
BpuMI CCSGG 1 cut(s) 405
Bsa29I ATCGAT 1 cut(s) 918
BsaJI CCNNGG 3 cut(s) 90, 594, 603
BsaXI ACNNNNNCTCC 2 cut(s) 474, 504
Bsc4I CCNNNNNNNGG 5 cut(s) 96, 141, 329, 404, 656
Bse1I ACTGG 1 cut(s) 701
BseCI ATCGAT 1 cut(s) 918
BseDI CCNNGG 3 cut(s) 90, 594, 603
BseGI GGATG 2 cut(s) 613, 783
BseLI CCNNNNNNNGG 5 cut(s) 96, 141, 329, 404, 656
BseMII CTCAG 1 cut(s) 705
BseNI ACTGG 1 cut(s) 701
BseRI GAGGAG 1 cut(s) 635
BseSI GKGCMC 1 cut(s) 602
BseXI GCAGC 2 cut(s) 577, 821
BseYI CCCAGC 3 cut(s) 29, 353, 399
BsgI GTGCAG 1 cut(s) 567
BshFI GGCC 2 cut(s) 81, 458
BshNI GGYRCC 1 cut(s) 599
BshVI ATCGAT 1 cut(s) 918
BsiHKAI GWGCWC 2 cut(s) 294, 700
BsiSI CCGG 2 cut(s) 135, 405
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 5 cut(s) 96, 141, 329, 404, 656
BsmAI GTCTC 1 cut(s) 779
BsmFI GGGAC 1 cut(s) 380
BsnI GGCC 2 cut(s) 81, 458
Bsp1286I GDGCHC 4 cut(s) 102, 294, 602, 700
Bsp143I GATC 2 cut(s) 52, 561
Bsp19I CCATGG 1 cut(s) 603
BspACI CCGC 1 cut(s) 341
BspANI GGCC 2 cut(s) 81, 458
BspCNI CTCAG 1 cut(s) 706
BspDI ATCGAT 1 cut(s) 918
BspHI TCATGA 1 cut(s) 568
BspLI GGNNCC 2 cut(s) 601, 708
BspMI ACCTGC 1 cut(s) 320
BspQI GCTCTTC 1 cut(s) 282
BspT107I GGYRCC 1 cut(s) 599
BsrI ACTGG 1 cut(s) 701
BssECI CCNNGG 3 cut(s) 90, 594, 603
BssMI GATC 2 cut(s) 52, 561
BssT1I CCWWGG 3 cut(s) 90, 594, 603
Bst4CI ACNGT 3 cut(s) 364, 496, 625
Bst6I CTCTTC 1 cut(s) 282
BstC8I GCNNGC 1 cut(s) 662
BstDEI CTNAG 1 cut(s) 714
BstDSI CCRYGG 1 cut(s) 603
BstF5I GGATG 2 cut(s) 613, 783
BstKTI GATC 2 cut(s) 55, 564
BstMAI GTCTC 1 cut(s) 779
BstMBI GATC 2 cut(s) 52, 561
BstMWI GCNNNNNNNGC 5 cut(s) 177, 335, 482, 715, 764
BstSCI CCNGG 1 cut(s) 403
BstSLI GKGCMC 1 cut(s) 602
BstV1I GCAGC 2 cut(s) 577, 821
BstV2I GAAGAC 1 cut(s) 718
Bsu15I ATCGAT 1 cut(s) 918
BsuRI GGCC 2 cut(s) 81, 458
BsuTUI ATCGAT 1 cut(s) 918
BtgI CCRYGG 1 cut(s) 603
BtsCI GGATG 2 cut(s) 613, 783
BtsI GCAGTG 2 cut(s) 537, 864
BtsIMutI CAGTG 6 cut(s) 232, 492, 537, 621, 708, 864
BveI ACCTGC 1 cut(s) 320
Cac8I GCNNGC 1 cut(s) 662
CciI TCATGA 1 cut(s) 568
Cfr13I GGNCC 1 cut(s) 219
ClaI ATCGAT 1 cut(s) 918
CviAII CATG 7 cut(s) 68, 148, 159, 168, 569, 575, 604
DdeI CTNAG 1 cut(s) 714
DpnI GATC 2 cut(s) 54, 563
DpnII GATC 2 cut(s) 52, 561
EaeI YGGCCR 2 cut(s) 79, 456
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Eco130I CCWWGG 3 cut(s) 90, 594, 603
Eco24I GRGCYC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 219
Eco57I CTGAAG 1 cut(s) 208
EcoT14I CCWWGG 3 cut(s) 90, 594, 603
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 3 cut(s) 90, 594, 603
FaeI CATG 7 cut(s) 71, 151, 162, 171, 572, 578, 607
FaqI GGGAC 1 cut(s) 380
FatI CATG 7 cut(s) 67, 147, 158, 167, 568, 574, 603
FauI CCCGC 1 cut(s) 348
FauNDI CATATG 1 cut(s) 453
FbaI TGATCA 2 cut(s) 52, 561
Fnu4HI GCNGC 2 cut(s) 591, 810
FokI GGATG 2 cut(s) 620, 790
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 2 cut(s) 591, 810
FspBI CTAG 5 cut(s) 326, 392, 672, 683, 949
GluI GCNGC 2 cut(s) 591, 810
GsaI CCCAGC 3 cut(s) 33, 357, 403
GsuI CTGGAG 1 cut(s) 684
HaeIII GGCC 2 cut(s) 81, 458
HapII CCGG 2 cut(s) 135, 405
Hin1II CATG 7 cut(s) 71, 151, 162, 171, 572, 578, 607
HindIII AAGCTT 1 cut(s) 474
HinfI GANTC 3 cut(s) 306, 551, 787
HpaII CCGG 2 cut(s) 135, 405
Hpy166II GTNNAC 2 cut(s) 230, 862
Hpy188I TCNGA 4 cut(s) 305, 389, 482, 513
Hpy188III TCNNGA 3 cut(s) 569, 791, 872
Hpy8I GTNNAC 2 cut(s) 230, 862
HpyAV CCTTC 1 cut(s) 232
HpyCH4III ACNGT 3 cut(s) 364, 496, 625
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 6 cut(s) 147, 180, 548, 767, 809, 944
HpyF10VI GCNNNNNNNGC 5 cut(s) 177, 335, 482, 715, 764
HpyF3I CTNAG 1 cut(s) 714
HpySE526I ACGT 1 cut(s) 105
Hsp92II CATG 7 cut(s) 71, 151, 162, 171, 572, 578, 607
Ksp22I TGATCA 2 cut(s) 52, 561
Kzo9I GATC 2 cut(s) 52, 561
LguI GCTCTTC 1 cut(s) 282
LmnI GCTCC 3 cut(s) 482, 703, 706
Lsp1109I GCAGC 2 cut(s) 577, 821
MaeI CTAG 5 cut(s) 326, 392, 672, 683, 949
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 3 cut(s) 3, 85, 490
MalI GATC 2 cut(s) 54, 563
MboI GATC 2 cut(s) 52, 561
MboII GAAGA 3 cut(s) 251, 299, 718
MfeI CAATTG 1 cut(s) 768
MhlI GDGCHC 4 cut(s) 102, 294, 602, 700
MlsI TGGCCA 1 cut(s) 81
MluCI AATT 7 cut(s) 44, 72, 113, 196, 581, 735, 768
MluNI TGGCCA 1 cut(s) 81
MlyI GAGTC 1 cut(s) 781
MmeI TCCRAC 1 cut(s) 103
MnlI CCTC 5 cut(s) 434, 517, 580, 656, 738
Mox20I TGGCCA 1 cut(s) 81
MroXI GAANNNNTTC 2 cut(s) 383, 843
MscI TGGCCA 1 cut(s) 81
MseI TTAA 2 cut(s) 734, 900
MslI CAYNNNNRTG 2 cut(s) 423, 573
Msp20I TGGCCA 1 cut(s) 81
MspI CCGG 2 cut(s) 135, 405
MspR9I CCNGG 1 cut(s) 405
MunI CAATTG 1 cut(s) 768
MwoI GCNNNNNNNGC 5 cut(s) 177, 335, 482, 715, 764
NciI CCSGG 1 cut(s) 405
NcoI CCATGG 1 cut(s) 603
NdeI CATATG 1 cut(s) 453
NdeII GATC 2 cut(s) 52, 561
NlaIII CATG 7 cut(s) 71, 151, 162, 171, 572, 578, 607
NlaIV GGNNCC 2 cut(s) 601, 708
NmuCI GTSAC 3 cut(s) 3, 85, 490
PagI TCATGA 1 cut(s) 568
PaqCI CACCTGC 1 cut(s) 320
PciSI GCTCTTC 1 cut(s) 282
PdmI GAANNNNTTC 2 cut(s) 383, 843
PfeI GAWTC 2 cut(s) 306, 551
PflMI CCANNNNNTGG 1 cut(s) 656
PkrI GCNGC 2 cut(s) 592, 811
PleI GAGTC 1 cut(s) 781
PpsI GAGTC 1 cut(s) 781
PspFI CCCAGC 3 cut(s) 29, 353, 399
PspN4I GGNNCC 2 cut(s) 601, 708
PspPI GGNCC 1 cut(s) 219
RseI CAYNNNNRTG 2 cut(s) 423, 573
SapI GCTCTTC 1 cut(s) 282
SaqAI TTAA 2 cut(s) 734, 900
SatI GCNGC 2 cut(s) 591, 810
Sau3AI GATC 2 cut(s) 52, 561
Sau96I GGNCC 1 cut(s) 219
SchI GAGTC 1 cut(s) 781
ScrFI CCNGG 1 cut(s) 405
SduI GDGCHC 4 cut(s) 102, 294, 602, 700
SinI GGWCC 1 cut(s) 219
SmiMI CAYNNNNRTG 2 cut(s) 423, 573
SmlI CTYRAG 2 cut(s) 789, 881
SmoI CTYRAG 2 cut(s) 789, 881
SpeI ACTAGT 3 cut(s) 391, 671, 682
Sse9I AATT 7 cut(s) 44, 72, 113, 196, 581, 735, 768
SsiI CCGC 1 cut(s) 341
SspI AATATT 1 cut(s) 383
SspMI CTAG 5 cut(s) 326, 392, 672, 683, 949
StyD4I CCNGG 1 cut(s) 403
StyI CCWWGG 3 cut(s) 90, 594, 603
TaaI ACNGT 3 cut(s) 364, 496, 625
TaiI ACGT 1 cut(s) 108
TaqI TCGA 3 cut(s) 618, 837, 918
TasI AATT 7 cut(s) 44, 72, 113, 196, 581, 735, 768
TfiI GAWTC 2 cut(s) 306, 551
Tru1I TTAA 2 cut(s) 734, 900
Tru9I TTAA 2 cut(s) 734, 900
TscAI CASTG 6 cut(s) 232, 499, 537, 628, 708, 871
TseFI GTSAC 3 cut(s) 3, 85, 490
TseI GCWGC 2 cut(s) 590, 809
Tsp45I GTSAC 3 cut(s) 3, 85, 490
TspDTI ATGAA 5 cut(s) 84, 147, 252, 543, 794
TspRI CASTG 6 cut(s) 232, 499, 537, 628, 708, 871
Van91I CCANNNNNTGG 1 cut(s) 656
VpaK11BI GGWCC 1 cut(s) 219
XapI RAATTY 1 cut(s) 113
XmnI GAANNNNTTC 2 cut(s) 383, 843
XspI CTAG 5 cut(s) 326, 392, 672, 683, 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.