RchiOBHm_Chr2g0128501

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000862
Physical Location & Seq
Forward (+)
43613662 .. 43614675
1014 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 861 bp
ATGGGAGGGTGCTTTTCGGACGTCAGAGGAGGGAAGCAGGCAGTAGGGGGAGCGCAACAAAGGCCAATTAACTACCATGAGGATTGCAACGACGCCGTTGACTGCTTTAACAAGTCTCAGGGAGCTGTCCCACTATTCACTCCACTCGAGCTATCCTTCTCCGCCTCTAACTTACTTGATCGTGATTTCACTTCGAAGAGTGATCCCATGGTTAAAGTTAGAGTTGCATATCATTTCGAGTTTGTACAGCCGTTGCTATTCCGTGTGTATGATGTCGACACAAAATATCACAACGTAAATGTGAAGACACTGGATTTGAAGAATCAAGAATTTCTAGGGGAAGGCACTTGTGCTCTTTCGGAGATAGTGACTAAACAAAGTCGAAGTCTTACCCTAAATCTCAATGATGACTATGGACGTCTTGGAGGCCGGAGAAACCTAGGAACACTCACTGTACATGCTGAGGAATTTAGTGCTTCTAAGAGTGTTGTTGAGATAAAATTTCGTTGTTCCCGCTTGGAAAACAAAGACCTGTTTTCTAAAAGCGATCCATTCTTAAGAATATCGAGGATGGTTGAGACCGGTGGTTGTGTTCCAATTTGCAAGACAGAAGTGGTGGACAACAATTTAAATCCAACTTGGAAATCACTGTGCCTGAGTATGCAGCAGATTGGAAGCAAGGATAATCCCTTAGTTATTGAATGCTTTGATTTCAACAGCAATGGCGAACATGTTCTTATTGGAAAACTCCAAAAGTCGGTGGCAGACCTCGAGAAACTCTACAAAGAGAAAAGCAGCGTCAACTTTGTCATTCCGCCATCTTCTCGTCATGGTCATGAAAAGCTCTCCAAGGTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

286

Amino Acids

31.96

Weight (kDa)

7.56

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 169 - 259 4.1e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 24, 421
AccI GTMKAC 1 cut(s) 276
AciI CCGC 3 cut(s) 162, 514, 815
AclWI GGATC 2 cut(s) 197, 542
AcsI RAATTY 3 cut(s) 329, 467, 500
AcyI GRCGYC 3 cut(s) 21, 93, 418
AfaI GTAC 2 cut(s) 246, 456
AfiI CCNNNNNNNGG 1 cut(s) 757
AflII CTTAAG 1 cut(s) 556
AflIII ACRYGT 1 cut(s) 730
AgeI ACCGGT 1 cut(s) 581
AgsI TTSAA 3 cut(s) 319, 701, 715
AluBI AGCT 3 cut(s) 125, 151, 844
AluI AGCT 3 cut(s) 125, 151, 844
Alw21I GWGCWC 1 cut(s) 355
Alw26I GTCTC 2 cut(s) 120, 572
AlwI GGATC 2 cut(s) 197, 542
Ama87I CYCGRG 2 cut(s) 146, 770
AoxI GGCC 2 cut(s) 62, 427
ApeKI GCWGC 2 cut(s) 664, 795
ApoI RAATTY 3 cut(s) 329, 467, 500
ArsI GACNNNNNNTTYG 4 cut(s) 298, 330, 370, 402
AsiGI ACCGGT 1 cut(s) 581
Asp700I GAANNNNTTC 1 cut(s) 732
AspA2I CCTAGG 1 cut(s) 439
AspLEI GCGC 1 cut(s) 55
AsuII TTCGAA 1 cut(s) 194
AvaI CYCGRG 2 cut(s) 146, 770
AvrII CCTAGG 1 cut(s) 439
BbsI GAAGAC 1 cut(s) 311
Bbv12I GWGCWC 1 cut(s) 355
BbvCI CCTCAGC 1 cut(s) 462
BbvI GCAGC 2 cut(s) 676, 807
BccI CCATC 2 cut(s) 565, 826
BceAI ACGGC 2 cut(s) 80, 235
BcoDI GTCTC 2 cut(s) 120, 572
BfaI CTAG 2 cut(s) 335, 440
BfrI CTTAAG 1 cut(s) 556
BisI GCNGC 2 cut(s) 665, 796
BlnI CCTAGG 1 cut(s) 439
BlsI GCNGC 2 cut(s) 666, 797
BmeT110I CYCGRG 2 cut(s) 146, 770
BpiI GAAGAC 1 cut(s) 311
Bpu10I CCTNAGC 1 cut(s) 462
Bpu14I TTCGAA 1 cut(s) 194
BsaHI GRCGYC 3 cut(s) 21, 93, 418
BsaI GGTCTC 1 cut(s) 572
BsaJI CCNNGG 3 cut(s) 207, 439, 849
BsaWI WCCGGW 1 cut(s) 581
Bsc4I CCNNNNNNNGG 1 cut(s) 757
Bse118I RCCGGY 1 cut(s) 581
Bse1I ACTGG 1 cut(s) 315
Bse3DI GCAATG 1 cut(s) 727
BseDI CCNNGG 3 cut(s) 207, 439, 849
BseGI GGATG 1 cut(s) 576
BseLI CCNNNNNNNGG 1 cut(s) 757
BseMI GCAATG 1 cut(s) 727
BseMII CTCAG 3 cut(s) 131, 453, 647
BseNI ACTGG 1 cut(s) 315
BseRI GAGGAG 1 cut(s) 42
BseXI GCAGC 2 cut(s) 676, 807
BshFI GGCC 2 cut(s) 64, 429
BshTI ACCGGT 1 cut(s) 581
BsiHKAI GWGCWC 1 cut(s) 355
BsiHKCI CYCGRG 2 cut(s) 146, 770
BsiSI CCGG 2 cut(s) 430, 582
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 757
BsmAI GTCTC 2 cut(s) 120, 572
BsmFI GGGAC 1 cut(s) 113
BsmI GAATGC 1 cut(s) 707
BsnI GGCC 2 cut(s) 64, 429
Bso31I GGTCTC 1 cut(s) 572
BsoBI CYCGRG 2 cut(s) 146, 770
Bsp119I TTCGAA 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 355
Bsp1407I TGTACA 2 cut(s) 244, 454
Bsp143I GATC 3 cut(s) 178, 202, 547
Bsp19I CCATGG 1 cut(s) 207
BspACI CCGC 3 cut(s) 162, 514, 815
BspANI GGCC 2 cut(s) 64, 429
BspCNI CTCAG 3 cut(s) 130, 454, 648
BspHI TCATGA 1 cut(s) 835
BspPI GGATC 2 cut(s) 197, 542
BspT104I TTCGAA 1 cut(s) 194
BspTI CTTAAG 1 cut(s) 556
BspTNI GGTCTC 1 cut(s) 572
BsrDI GCAATG 1 cut(s) 727
BsrFI RCCGGY 1 cut(s) 581
BsrGI TGTACA 2 cut(s) 244, 454
BsrI ACTGG 1 cut(s) 315
BssAI RCCGGY 1 cut(s) 581
BssECI CCNNGG 3 cut(s) 207, 439, 849
BssMI GATC 3 cut(s) 178, 202, 547
BssNI GRCGYC 3 cut(s) 21, 93, 418
BssT1I CCWWGG 3 cut(s) 207, 439, 849
Bst4CI ACNGT 2 cut(s) 454, 651
Bst6I CTCTTC 1 cut(s) 191
BstACI GRCGYC 3 cut(s) 21, 93, 418
BstAFI CTTAAG 1 cut(s) 556
BstAUI TGTACA 2 cut(s) 244, 454
BstBI TTCGAA 1 cut(s) 194
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 5 cut(s) 117, 462, 480, 656, 691
BstDSI CCRYGG 1 cut(s) 207
BstF5I GGATG 1 cut(s) 576
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 3 cut(s) 181, 205, 550
BstMAI GTCTC 2 cut(s) 120, 572
BstMBI GATC 3 cut(s) 178, 202, 547
BstMWI GCNNNNNNNGC 1 cut(s) 61
BstNSI RCATGY 2 cut(s) 461, 734
BstV1I GCAGC 2 cut(s) 676, 807
BstV2I GAAGAC 1 cut(s) 311
BsuRI GGCC 2 cut(s) 64, 429
BtgI CCRYGG 1 cut(s) 207
BtsCI GGATG 1 cut(s) 576
BtsIMutI CAGTG 3 cut(s) 308, 450, 647
Cac8I GCNNGC 1 cut(s) 39
CciI TCATGA 1 cut(s) 835
CfoI GCGC 1 cut(s) 55
Cfr10I RCCGGY 1 cut(s) 581
CseI GACGC 2 cut(s) 101, 787
Csp6I GTAC 2 cut(s) 245, 455
CspAI ACCGGT 1 cut(s) 581
CviAII CATG 6 cut(s) 77, 208, 458, 731, 830, 836
CviJI RGCY 6 cut(s) 64, 125, 151, 250, 429, 844
CviKI_1 RGCY 6 cut(s) 64, 125, 151, 250, 429, 844
CviQI GTAC 2 cut(s) 245, 455
DdeI CTNAG 5 cut(s) 117, 462, 480, 656, 691
DpnI GATC 3 cut(s) 180, 204, 549
DpnII GATC 3 cut(s) 178, 202, 547
DraI TTTAAA 1 cut(s) 630
Eam1104I CTCTTC 1 cut(s) 191
EarI CTCTTC 1 cut(s) 191
EciI GGCGGA 2 cut(s) 151, 804
Eco130I CCWWGG 3 cut(s) 207, 439, 849
Eco31I GGTCTC 1 cut(s) 572
Eco88I CYCGRG 2 cut(s) 146, 770
EcoT14I CCWWGG 3 cut(s) 207, 439, 849
ErhI CCWWGG 3 cut(s) 207, 439, 849
FaeI CATG 6 cut(s) 80, 211, 461, 734, 833, 839
FaqI GGGAC 1 cut(s) 113
FatI CATG 6 cut(s) 76, 207, 457, 730, 829, 835
FauI CCCGC 1 cut(s) 521
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 2 cut(s) 665, 796
FokI GGATG 1 cut(s) 583
Fsp4HI GCNGC 2 cut(s) 665, 796
FspBI CTAG 2 cut(s) 335, 440
GlaI GCGC 1 cut(s) 54
GluI GCNGC 2 cut(s) 665, 796
HaeIII GGCC 2 cut(s) 64, 429
HapII CCGG 2 cut(s) 430, 582
HgaI GACGC 2 cut(s) 101, 787
HhaI GCGC 1 cut(s) 55
Hin1I GRCGYC 3 cut(s) 21, 93, 418
Hin1II CATG 6 cut(s) 80, 211, 461, 734, 833, 839
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HincII GTYRAC 3 cut(s) 100, 277, 802
HindII GTYRAC 3 cut(s) 100, 277, 802
HinfI GANTC 1 cut(s) 322
HpaII CCGG 2 cut(s) 430, 582
Hpy166II GTNNAC 4 cut(s) 100, 277, 619, 802
Hpy188I TCNGA 3 cut(s) 19, 26, 361
Hpy188III TCNNGA 4 cut(s) 182, 326, 772, 836
Hpy8I GTNNAC 4 cut(s) 100, 277, 619, 802
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 2 cut(s) 166, 335
HpyCH4III ACNGT 2 cut(s) 454, 651
HpyCH4IV ACGT 3 cut(s) 21, 294, 418
HpyCH4V TGCA 4 cut(s) 87, 227, 603, 664
HpyF10VI GCNNNNNNNGC 1 cut(s) 61
HpyF3I CTNAG 5 cut(s) 117, 462, 480, 656, 691
HpySE526I ACGT 3 cut(s) 21, 294, 418
Hsp92I GRCGYC 3 cut(s) 21, 93, 418
Hsp92II CATG 6 cut(s) 80, 211, 461, 734, 833, 839
HspAI GCGC 1 cut(s) 53
Kzo9I GATC 3 cut(s) 178, 202, 547
LmnI GCTCC 2 cut(s) 50, 122
LpnPI CCDG 7 cut(s) 23, 104, 296, 443, 545, 595, 668
Lsp1109I GCAGC 2 cut(s) 676, 807
MaeI CTAG 2 cut(s) 335, 440
MaeII ACGT 3 cut(s) 21, 294, 418
MaeIII GTNAC 1 cut(s) 367
MalI GATC 3 cut(s) 180, 204, 549
MboI GATC 3 cut(s) 178, 202, 547
MboII GAAGA 4 cut(s) 208, 316, 331, 813
MhlI GDGCHC 1 cut(s) 355
MluCI AATT 6 cut(s) 66, 329, 467, 500, 597, 625
MmeI TCCRAC 1 cut(s) 659
MnlI CCTC 8 cut(s) 20, 23, 73, 175, 419, 457, 561, 779
MroXI GAANNNNTTC 1 cut(s) 732
MseI TTAA 5 cut(s) 69, 108, 213, 557, 629
MslI CAYNNNNRTG 1 cut(s) 834
MspCI CTTAAG 1 cut(s) 556
MspI CCGG 2 cut(s) 430, 582
Mva1269I GAATGC 1 cut(s) 707
MwoI GCNNNNNNNGC 1 cut(s) 61
NcoI CCATGG 1 cut(s) 207
NdeII GATC 3 cut(s) 178, 202, 547
NlaIII CATG 6 cut(s) 80, 211, 461, 734, 833, 839
NmuCI GTSAC 1 cut(s) 367
NspI RCATGY 2 cut(s) 461, 734
NspV TTCGAA 1 cut(s) 194
PaeR7I CTCGAG 2 cut(s) 146, 770
PagI TCATGA 1 cut(s) 835
PciI ACATGT 1 cut(s) 730
PctI GAATGC 1 cut(s) 707
PdmI GAANNNNTTC 1 cut(s) 732
PfeI GAWTC 1 cut(s) 322
PinAI ACCGGT 1 cut(s) 581
PkrI GCNGC 2 cut(s) 666, 797
PscI ACATGT 1 cut(s) 730
PspXI VCTCGAGB 1 cut(s) 146
RsaI GTAC 2 cut(s) 246, 456
RsaNI GTAC 2 cut(s) 245, 455
RseI CAYNNNNRTG 1 cut(s) 834
SalI GTCGAC 1 cut(s) 275
SaqAI TTAA 5 cut(s) 69, 108, 213, 557, 629
SatI GCNGC 2 cut(s) 665, 796
Sau3AI GATC 3 cut(s) 178, 202, 547
SduI GDGCHC 1 cut(s) 355
Sfr274I CTCGAG 2 cut(s) 146, 770
SfuI TTCGAA 1 cut(s) 194
SlaI CTCGAG 2 cut(s) 146, 770
SmiI ATTTAAAT 1 cut(s) 630
SmiMI CAYNNNNRTG 1 cut(s) 834
SmlI CTYRAG 3 cut(s) 146, 556, 770
SmoI CTYRAG 3 cut(s) 146, 556, 770
Sse9I AATT 6 cut(s) 66, 329, 467, 500, 597, 625
SsiI CCGC 3 cut(s) 162, 514, 815
SspMI CTAG 2 cut(s) 335, 440
StyI CCWWGG 3 cut(s) 207, 439, 849
SwaI ATTTAAAT 1 cut(s) 630
TaaI ACNGT 2 cut(s) 454, 651
TaiI ACGT 3 cut(s) 24, 297, 421
TaqI TCGA 7 cut(s) 147, 194, 237, 276, 382, 566, 771
TasI AATT 6 cut(s) 66, 329, 467, 500, 597, 625
TatI WGTACW 2 cut(s) 244, 454
TfiI GAWTC 1 cut(s) 322
Tru1I TTAA 5 cut(s) 69, 108, 213, 557, 629
Tru9I TTAA 5 cut(s) 69, 108, 213, 557, 629
TscAI CASTG 3 cut(s) 315, 457, 654
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 2 cut(s) 664, 795
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 1 cut(s) 852
TspGWI ACGGA 1 cut(s) 251
TspRI CASTG 3 cut(s) 315, 457, 654
Vha464I CTTAAG 1 cut(s) 556
XapI RAATTY 3 cut(s) 329, 467, 500
XceI RCATGY 2 cut(s) 461, 734
XhoI CTCGAG 2 cut(s) 146, 770
XmaJI CCTAGG 1 cut(s) 439
XmiI GTMKAC 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 732
XspI CTAG 2 cut(s) 335, 440
ZraI GACGTC 2 cut(s) 22, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.