RchiOBHm_Chr1g0342201

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001265
Physical Location & Seq
Reverse (-)
34139285 .. 34140553
1269 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 831 bp
ATGTCGACCTTGGCCGATCCCAAAGCTCACCACCATGAATTTGTTATCCAAGCAACACCAGTGAAGAGGCTGTGCAAAACCCACAACACCATCACAGTGAATGGACAGTTTCCTGGACCAACCTTGGAAGTAAACAATGGCGACACTCTGGTCGTCAAAGTCACTAACAAAGCTCGATATAACGTCACCATTCACTGTTACACCTATCGGTTTACAGTTCAGGGTCAAGAAGGTACTCTGTGGTGGCACGCTCATAGCTCATGGCTTAGAGCCACCGTGTATGGAGCACTTATTATTCATCCGAAACAAGGAGACTCGTATCCATTCACTAAACCGAATCGTCAAGCAACACTTCTTCTAGGTGAGTGGTGGGATGGTAACCCTATTGACGTGGTGAGGAGGGCGACTCGCACAGGAGCAGTTCCAAATGTTTCTAACGCATATACCATTAACGGTCAACCCGGAGATCTTTACAGTTGCTCCAGCAAAGGTTTGATTCAAACTCCCATTGGCACCGTCATAGTTCCAATAGACTCTGGTGAGACCAACCTACTGAGAGTGATAAACGCCGCCCTCAACCAACCTCTTTTCTTCTCCGTCGCCAACCATAAACTGACTGTGGTTGGTGCTGATGCCTCGTACACCAAGCCTTTCACCACCAAAGTTCTCATGCTAGGCCCAGACCAGACTACTGATGTTTTAATCACCGGGGACCAGCCACCATCTCGATACTACTTGGCAGCGAGTGCCTATTTCAGTGCACAAAATGCTCCGTTTGACAACACCACCACCACAGCCATCAGGGCCGGTCCTGAGATTCTAACGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

276

Amino Acids

30.19

Weight (kDa)

8.91

Isoelectric Point (pI)

25.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 16 - 65 1.5e-12 Multicopper oxidase
Cu-oxidase_3 PF07732 67 - 103 1.9e-09 Multicopper oxidase
Cu-oxidase PF00394 116 - 267 6.2e-36 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
AccB1I GGYRCC 1 cut(s) 512
AccI GTMKAC 1 cut(s) 5
AciI CCGC 1 cut(s) 570
AclWI GGATC 1 cut(s) 11
AcoI YGGCCR 1 cut(s) 12
AcsI RAATTY 1 cut(s) 38
AfaI GTAC 2 cut(s) 235, 641
AfiI CCNNNNNNNGG 1 cut(s) 308
AgsI TTSAA 1 cut(s) 500
AjiI CACGTC 1 cut(s) 391
AjnI CCWGG 1 cut(s) 112
AluBI AGCT 3 cut(s) 26, 173, 258
AluI AGCT 3 cut(s) 26, 173, 258
Alw21I GWGCWC 2 cut(s) 289, 763
Alw26I GTCTC 2 cut(s) 306, 536
Alw44I GTGCAC 1 cut(s) 759
AlwI GGATC 1 cut(s) 11
AoxI GGCC 4 cut(s) 12, 676, 804, 825
ApaLI GTGCAC 1 cut(s) 759
ApeKI GCWGC 1 cut(s) 740
ApoI RAATTY 1 cut(s) 38
AspS9I GGNCC 5 cut(s) 116, 677, 712, 804, 809
AsuC2I CCSGG 2 cut(s) 462, 709
AsuHPI GGTGA 7 cut(s) 20, 178, 374, 406, 551, 646, 697
AvaII GGWCC 3 cut(s) 116, 712, 809
BaeGI GKGCMC 1 cut(s) 763
BanI GGYRCC 1 cut(s) 512
Bbv12I GWGCWC 2 cut(s) 289, 763
BbvI GCAGC 1 cut(s) 752
BccI CCATC 4 cut(s) 98, 368, 730, 806
BciT130I CCWGG 1 cut(s) 114
BciVI GTATCC 1 cut(s) 330
BcnI CCSGG 2 cut(s) 462, 709
BcoDI GTCTC 2 cut(s) 306, 536
BfaI CTAG 2 cut(s) 359, 674
BfuI GTATCC 1 cut(s) 330
BglI GCCNNNNNGGC 1 cut(s) 803
BglII AGATCT 1 cut(s) 466
BisI GCNGC 2 cut(s) 570, 741
BlsI GCNGC 2 cut(s) 571, 742
Bme1390I CCNGG 3 cut(s) 114, 462, 709
Bme18I GGWCC 3 cut(s) 116, 712, 809
BmgBI CACGTC 1 cut(s) 391
BmgT120I GGNCC 5 cut(s) 116, 677, 712, 804, 809
BmiI GGNNCC 2 cut(s) 514, 713
BmrFI CCNGG 3 cut(s) 114, 462, 709
BmsI GCATC 1 cut(s) 622
BplI GAGNNNNNCTC 2 cut(s) 391, 423
BpmI CTGGAG 1 cut(s) 466
BpuMI CCSGG 2 cut(s) 462, 709
BsaI GGTCTC 1 cut(s) 536
BsaJI CCNNGG 3 cut(s) 9, 123, 708
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bsc4I CCNNNNNNNGG 1 cut(s) 308
Bse118I RCCGGY 1 cut(s) 806
Bse1I ACTGG 1 cut(s) 59
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 3 cut(s) 9, 123, 708
BseGI GGATG 2 cut(s) 298, 379
BseLI CCNNNNNNNGG 1 cut(s) 308
BseMII CTCAG 2 cut(s) 545, 804
BseNI ACTGG 1 cut(s) 59
BseRI GAGGAG 1 cut(s) 412
BseSI GKGCMC 1 cut(s) 763
BseXI GCAGC 1 cut(s) 752
BshFI GGCC 4 cut(s) 14, 678, 806, 827
BshNI GGYRCC 1 cut(s) 512
BsiHKAI GWGCWC 2 cut(s) 289, 763
BsiSI CCGG 3 cut(s) 462, 708, 807
BslFI GGGAC 1 cut(s) 725
BslI CCNNNNNNNGG 1 cut(s) 308
BsmAI GTCTC 2 cut(s) 306, 536
BsmFI GGGAC 1 cut(s) 725
BsnI GGCC 4 cut(s) 14, 678, 806, 827
Bso31I GGTCTC 1 cut(s) 536
Bsp1286I GDGCHC 2 cut(s) 289, 763
Bsp143I GATC 2 cut(s) 16, 466
BspACI CCGC 1 cut(s) 570
BspANI GGCC 4 cut(s) 14, 678, 806, 827
BspCNI CTCAG 2 cut(s) 546, 805
BspLI GGNNCC 2 cut(s) 514, 713
BspPI GGATC 1 cut(s) 11
BspT107I GGYRCC 1 cut(s) 512
BspTNI GGTCTC 1 cut(s) 536
BsrFI RCCGGY 1 cut(s) 806
BsrI ACTGG 1 cut(s) 59
BssAI RCCGGY 1 cut(s) 806
BssECI CCNNGG 3 cut(s) 9, 123, 708
BssMI GATC 2 cut(s) 16, 466
BssT1I CCWWGG 2 cut(s) 9, 123
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 9 cut(s) 97, 108, 197, 217, 277, 455, 476, 517, 619
Bst6I CTCTTC 1 cut(s) 59
BstAPI GCANNNNNTGC 2 cut(s) 746, 767
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 3 cut(s) 266, 554, 813
BstEII GGTNACC 1 cut(s) 377
BstF5I GGATG 2 cut(s) 298, 379
BstKTI GATC 2 cut(s) 19, 469
BstMAI GTCTC 2 cut(s) 306, 536
BstMBI GATC 2 cut(s) 16, 466
BstMWI GCNNNNNNNGC 3 cut(s) 746, 767, 803
BstNI CCWGG 1 cut(s) 114
BstPI GGTNACC 1 cut(s) 377
BstSCI CCNGG 3 cut(s) 112, 460, 707
BstSLI GKGCMC 1 cut(s) 763
BstV1I GCAGC 1 cut(s) 752
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BsuI GTATCC 1 cut(s) 330
BsuRI GGCC 4 cut(s) 14, 678, 806, 827
BtrI CACGTC 1 cut(s) 391
BtsCI GGATG 2 cut(s) 298, 379
BtsIMutI CAGTG 4 cut(s) 66, 102, 193, 763
Cac8I GCNNGC 1 cut(s) 249
Cfr10I RCCGGY 1 cut(s) 806
Cfr13I GGNCC 5 cut(s) 116, 677, 712, 804, 809
Csp6I GTAC 2 cut(s) 234, 640
CviAII CATG 3 cut(s) 35, 261, 670
CviQI GTAC 2 cut(s) 234, 640
DdeI CTNAG 3 cut(s) 266, 554, 813
DpnI GATC 2 cut(s) 18, 468
DpnII GATC 2 cut(s) 16, 466
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 1 cut(s) 12
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Eco130I CCWWGG 2 cut(s) 9, 123
Eco31I GGTCTC 1 cut(s) 536
Eco47I GGWCC 3 cut(s) 116, 712, 809
Eco91I GGTNACC 1 cut(s) 377
EcoO65I GGTNACC 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 112
EcoT14I CCWWGG 2 cut(s) 9, 123
ErhI CCWWGG 2 cut(s) 9, 123
FaeI CATG 3 cut(s) 38, 264, 673
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FaqI GGGAC 1 cut(s) 725
FatI CATG 3 cut(s) 34, 260, 669
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 2 cut(s) 570, 741
FokI GGATG 2 cut(s) 285, 386
Fsp4HI GCNGC 2 cut(s) 570, 741
FspBI CTAG 2 cut(s) 359, 674
GluI GCNGC 2 cut(s) 570, 741
GsuI CTGGAG 1 cut(s) 466
HaeIII GGCC 4 cut(s) 14, 678, 806, 827
HapII CCGG 3 cut(s) 462, 708, 807
Hin1II CATG 3 cut(s) 38, 264, 673
HincII GTYRAC 2 cut(s) 6, 458
HindII GTYRAC 2 cut(s) 6, 458
HinfI GANTC 6 cut(s) 314, 337, 406, 496, 533, 817
HpaII CCGG 3 cut(s) 462, 708, 807
HphI GGTGA 7 cut(s) 20, 178, 374, 406, 551, 646, 697
Hpy166II GTNNAC 6 cut(s) 6, 133, 213, 458, 642, 761
Hpy188I TCNGA 1 cut(s) 303
Hpy188III TCNNGA 3 cut(s) 227, 726, 812
Hpy8I GTNNAC 6 cut(s) 6, 133, 213, 458, 642, 761
Hpy99I CGWCG 1 cut(s) 602
HpyAV CCTTC 1 cut(s) 224
HpyCH4III ACNGT 9 cut(s) 97, 108, 197, 217, 277, 455, 476, 517, 619
HpyCH4IV ACGT 2 cut(s) 183, 390
HpyCH4V TGCA 2 cut(s) 75, 761
HpyF10VI GCNNNNNNNGC 3 cut(s) 746, 767, 803
HpyF3I CTNAG 3 cut(s) 266, 554, 813
HpySE526I ACGT 2 cut(s) 183, 390
Hsp92II CATG 3 cut(s) 38, 264, 673
Kzo9I GATC 2 cut(s) 16, 466
LmnI GCTCC 4 cut(s) 284, 416, 485, 775
Lsp1109I GCAGC 1 cut(s) 752
LweI GCATC 1 cut(s) 622
MaeI CTAG 2 cut(s) 359, 674
MaeII ACGT 2 cut(s) 183, 390
MaeIII GTNAC 4 cut(s) 160, 184, 197, 377
MalI GATC 2 cut(s) 18, 468
MboI GATC 2 cut(s) 16, 466
MboII GAAGA 3 cut(s) 76, 347, 583
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 2 cut(s) 289, 763
MluCI AATT 1 cut(s) 38
MlyI GAGTC 3 cut(s) 308, 400, 527
MnlI CCTC 6 cut(s) 60, 390, 393, 584, 594, 646
MseI TTAA 2 cut(s) 450, 701
MslI CAYNNNNRTG 2 cut(s) 33, 95
MspI CCGG 3 cut(s) 462, 708, 807
MspR9I CCNGG 3 cut(s) 114, 462, 709
MvaI CCWGG 1 cut(s) 114
MwoI GCNNNNNNNGC 3 cut(s) 746, 767, 803
NciI CCSGG 2 cut(s) 462, 709
NdeII GATC 2 cut(s) 16, 466
NlaIII CATG 3 cut(s) 38, 264, 673
NlaIV GGNNCC 2 cut(s) 514, 713
NmuCI GTSAC 2 cut(s) 160, 184
PfeI GAWTC 3 cut(s) 337, 496, 817
PfoI TCCNGGA 1 cut(s) 112
PkrI GCNGC 2 cut(s) 571, 742
PleI GAGTC 3 cut(s) 308, 400, 527
PpsI GAGTC 3 cut(s) 308, 400, 527
Psp6I CCWGG 1 cut(s) 112
PspEI GGTNACC 1 cut(s) 377
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 2 cut(s) 514, 713
PspPI GGNCC 5 cut(s) 116, 677, 712, 804, 809
PsuI RGATCY 1 cut(s) 466
RsaI GTAC 2 cut(s) 235, 641
RsaNI GTAC 2 cut(s) 234, 640
RseI CAYNNNNRTG 2 cut(s) 33, 95
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 450, 701
SatI GCNGC 2 cut(s) 570, 741
Sau3AI GATC 2 cut(s) 16, 466
Sau96I GGNCC 5 cut(s) 116, 677, 712, 804, 809
SchI GAGTC 3 cut(s) 308, 400, 527
ScrFI CCNGG 3 cut(s) 114, 462, 709
SduI GDGCHC 2 cut(s) 289, 763
SfaNI GCATC 1 cut(s) 622
SinI GGWCC 3 cut(s) 116, 712, 809
SmiMI CAYNNNNRTG 2 cut(s) 33, 95
Sse9I AATT 1 cut(s) 38
SsiI CCGC 1 cut(s) 570
SspMI CTAG 2 cut(s) 359, 674
StyD4I CCNGG 3 cut(s) 112, 460, 707
StyI CCWWGG 2 cut(s) 9, 123
TaaI ACNGT 9 cut(s) 97, 108, 197, 217, 277, 455, 476, 517, 619
TaiI ACGT 2 cut(s) 186, 393
TaqI TCGA 3 cut(s) 5, 175, 727
TasI AATT 1 cut(s) 38
TauI GCSGC 1 cut(s) 572
TfiI GAWTC 3 cut(s) 337, 496, 817
Tru1I TTAA 2 cut(s) 450, 701
Tru9I TTAA 2 cut(s) 450, 701
TscAI CASTG 4 cut(s) 66, 102, 200, 763
TseFI GTSAC 2 cut(s) 160, 184
TseI GCWGC 1 cut(s) 740
Tsp45I GTSAC 2 cut(s) 160, 184
TspDTI ATGAA 2 cut(s) 51, 287
TspGWI ACGGA 2 cut(s) 586, 762
TspRI CASTG 4 cut(s) 66, 102, 200, 763
VneI GTGCAC 1 cut(s) 759
VpaK11BI GGWCC 3 cut(s) 116, 712, 809
XapI RAATTY 1 cut(s) 38
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 359, 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.