RchiOBHm_Chr1g0359961

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002278
Physical Location & Seq
Reverse (-)
51763018 .. 51763662
645 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 564 bp
ATGGATTTTGCTTCCACGGCGGTAGCATTAAGTTTAGCCTTAGTTTTAGCTGTAGTTGCAGTTAGACACCGTGCCCGAAGATTGCATGACAAGAACAAGAGATACCATCCTGTTGCCGGAACTGTCGTGCACCAACTTATAAACTTCCCTAGGTTACACCATTACATGACTGAACTTGCGGGTAAGTATAACACTTACAGGATGCTCGACTTGTTCAAAGGTGCGGTTTACACAGCAGATCCTGCAAATGTGGAATATGTGCTCAAAACAAACTTCACTAATTATGGCAAGGGGTCGTATATGTACAACATTTTGTCAGATGGACTAGGTGATGGGATCTTCGCAGTGGATGGGCAAAAATGGCGGCATCAGAGGAAGGTGACGAGCACTGAATTCTCAACAAAAGTAGTGAGAGACTTCAGCAGTGCAGTTTTCAAAACCAATGCAGTTAAACTTGCTGGCATAATTTATGAAGCTGCAACCAGCTGCAGCAACCAAGCAATAGAGATCCAAGTATGTTTGCAACGTTTCAGTGTTTCACTAATCACATTGCATTGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

187

Amino Acids

21.03

Weight (kDa)

9.54

Isoelectric Point (pI)

25.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 45 - 183 4.9e-08 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 140
AciI CCGC 4 cut(s) 20, 179, 224, 364
AclI AACGTT 1 cut(s) 526
AclWI GGATC 3 cut(s) 233, 344, 502
AcsI RAATTY 1 cut(s) 392
AcuI CTGAAG 1 cut(s) 403
AfaI GTAC 1 cut(s) 305
AfiI CCNNNNNNNGG 1 cut(s) 116
AgsI TTSAA 2 cut(s) 217, 436
AluBI AGCT 3 cut(s) 50, 476, 486
AluI AGCT 3 cut(s) 50, 476, 486
Alw21I GWGCWC 3 cut(s) 132, 264, 389
Alw26I GTCTC 1 cut(s) 408
Alw44I GTGCAC 1 cut(s) 128
AlwI GGATC 3 cut(s) 233, 344, 502
AlwNI CAGNNNCTG 1 cut(s) 242
ApaLI GTGCAC 1 cut(s) 128
ApeKI GCWGC 3 cut(s) 476, 486, 489
ApoI RAATTY 1 cut(s) 392
AspA2I CCTAGG 1 cut(s) 149
AsuHPI GGTGA 2 cut(s) 341, 391
AvrII CCTAGG 1 cut(s) 149
BaeGI GKGCMC 2 cut(s) 76, 132
Bbv12I GWGCWC 3 cut(s) 132, 264, 389
BbvI GCAGC 3 cut(s) 463, 473, 501
BccI CCATC 4 cut(s) 114, 314, 326, 344
BceAI ACGGC 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 408
BfaI CTAG 2 cut(s) 150, 326
BfmI CTRYAG 2 cut(s) 51, 487
BisI GCNGC 4 cut(s) 365, 477, 487, 490
BlnI CCTAGG 1 cut(s) 149
BlsI GCNGC 4 cut(s) 366, 478, 488, 491
BmsI GCATC 2 cut(s) 192, 376
BsaJI CCNNGG 2 cut(s) 15, 149
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse3DI GCAATG 1 cut(s) 548
BseDI CCNNGG 2 cut(s) 15, 149
BseGI GGATG 3 cut(s) 106, 207, 355
BseLI CCNNNNNNNGG 1 cut(s) 116
BseMI GCAATG 1 cut(s) 548
BseSI GKGCMC 2 cut(s) 76, 132
BseXI GCAGC 3 cut(s) 463, 473, 501
BsgI GTGCAG 1 cut(s) 447
BsiHKAI GWGCWC 3 cut(s) 132, 264, 389
BsiSI CCGG 1 cut(s) 117
BslI CCNNNNNNNGG 1 cut(s) 116
BsmAI GTCTC 1 cut(s) 408
Bsp1286I GDGCHC 4 cut(s) 76, 132, 264, 389
Bsp1407I TGTACA 1 cut(s) 303
Bsp143I GATC 3 cut(s) 238, 336, 507
BspACI CCGC 4 cut(s) 20, 179, 224, 364
BspMAI CTGCAG 1 cut(s) 491
BspPI GGATC 3 cut(s) 233, 344, 502
BsrDI GCAATG 1 cut(s) 548
BsrGI TGTACA 1 cut(s) 303
BssECI CCNNGG 2 cut(s) 15, 149
BssMI GATC 3 cut(s) 238, 336, 507
BssT1I CCWWGG 1 cut(s) 149
Bst4CI ACNGT 2 cut(s) 71, 124
BstAPI GCANNNNNTGC 1 cut(s) 242
BstAUI TGTACA 1 cut(s) 303
BstC8I GCNNGC 1 cut(s) 460
BstDEI CTNAG 1 cut(s) 40
BstDSI CCRYGG 1 cut(s) 15
BstF5I GGATG 3 cut(s) 106, 207, 355
BstKTI GATC 3 cut(s) 241, 339, 510
BstMAI GTCTC 1 cut(s) 408
BstMBI GATC 3 cut(s) 238, 336, 507
BstMWI GCNNNNNNNGC 4 cut(s) 17, 56, 242, 361
BstSFI CTRYAG 2 cut(s) 51, 487
BstSLI GKGCMC 2 cut(s) 76, 132
BstV1I GCAGC 3 cut(s) 463, 473, 501
BstX2I RGATCY 3 cut(s) 238, 336, 507
BstYI RGATCY 3 cut(s) 238, 336, 507
BtgI CCRYGG 1 cut(s) 15
BtsCI GGATG 3 cut(s) 106, 207, 355
BtsI GCAGTG 2 cut(s) 351, 430
BtsIMutI CAGTG 4 cut(s) 351, 387, 430, 538
Cac8I GCNNGC 1 cut(s) 460
CaiI CAGNNNCTG 1 cut(s) 242
Csp6I GTAC 1 cut(s) 304
CviAII CATG 2 cut(s) 86, 166
CviJI RGCY 4 cut(s) 38, 50, 476, 486
CviKI_1 RGCY 4 cut(s) 38, 50, 476, 486
CviQI GTAC 1 cut(s) 304
DdeI CTNAG 1 cut(s) 40
DpnI GATC 3 cut(s) 240, 338, 509
DpnII GATC 3 cut(s) 238, 336, 507
Eco130I CCWWGG 1 cut(s) 149
Eco57I CTGAAG 1 cut(s) 403
EcoRI GAATTC 1 cut(s) 392
EcoT14I CCWWGG 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 149
FaeI CATG 2 cut(s) 89, 169
FatI CATG 2 cut(s) 85, 165
FauI CCCGC 1 cut(s) 172
Fnu4HI GCNGC 4 cut(s) 365, 477, 487, 490
FokI GGATG 3 cut(s) 93, 214, 362
Fsp4HI GCNGC 4 cut(s) 365, 477, 487, 490
FspBI CTAG 2 cut(s) 150, 326
GluI GCNGC 4 cut(s) 365, 477, 487, 490
HapII CCGG 1 cut(s) 117
Hin1II CATG 2 cut(s) 89, 169
HpaII CCGG 1 cut(s) 117
HphI GGTGA 2 cut(s) 341, 391
Hpy166II GTNNAC 2 cut(s) 130, 229
Hpy188I TCNGA 2 cut(s) 319, 372
Hpy8I GTNNAC 2 cut(s) 130, 229
HpyAV CCTTC 1 cut(s) 370
HpyCH4III ACNGT 2 cut(s) 71, 124
HpyCH4IV ACGT 1 cut(s) 526
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 56, 242, 361
HpyF3I CTNAG 1 cut(s) 40
HpySE526I ACGT 1 cut(s) 526
Hsp92II CATG 2 cut(s) 89, 169
Kzo9I GATC 3 cut(s) 238, 336, 507
LpnPI CCDG 6 cut(s) 123, 130, 184, 255, 444, 496
Lsp1109I GCAGC 3 cut(s) 463, 473, 501
LweI GCATC 2 cut(s) 192, 376
MaeI CTAG 2 cut(s) 150, 326
MaeII ACGT 1 cut(s) 526
MaeIII GTNAC 2 cut(s) 153, 379
MalI GATC 3 cut(s) 240, 338, 509
MboI GATC 3 cut(s) 238, 336, 507
MboII GAAGA 2 cut(s) 90, 331
MflI RGATCY 3 cut(s) 238, 336, 507
MhlI GDGCHC 4 cut(s) 76, 132, 264, 389
MluCI AATT 3 cut(s) 280, 392, 465
MnlI CCTC 1 cut(s) 366
MseI TTAA 3 cut(s) 29, 450, 562
MspA1I CMGCKG 1 cut(s) 486
MspI CCGG 1 cut(s) 117
MwoI GCNNNNNNNGC 4 cut(s) 17, 56, 242, 361
NdeII GATC 3 cut(s) 238, 336, 507
NlaIII CATG 2 cut(s) 89, 169
NmuCI GTSAC 1 cut(s) 379
PkrI GCNGC 4 cut(s) 366, 478, 488, 491
PsiI TTATAA 1 cut(s) 140
Psp1406I AACGTT 1 cut(s) 526
PsrI GAACNNNNNNTAC 2 cut(s) 86, 118
PstI CTGCAG 1 cut(s) 491
PstNI CAGNNNCTG 1 cut(s) 242
PsuI RGATCY 3 cut(s) 238, 336, 507
PvuII CAGCTG 1 cut(s) 486
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
SaqAI TTAA 3 cut(s) 29, 450, 562
SatI GCNGC 4 cut(s) 365, 477, 487, 490
Sau3AI GATC 3 cut(s) 238, 336, 507
SduI GDGCHC 4 cut(s) 76, 132, 264, 389
SetI ASST 8 cut(s) 52, 155, 223, 331, 381, 478, 488, 529
SfaNI GCATC 2 cut(s) 192, 376
SfcI CTRYAG 2 cut(s) 51, 487
Sse9I AATT 3 cut(s) 280, 392, 465
SsiI CCGC 4 cut(s) 20, 179, 224, 364
SspMI CTAG 2 cut(s) 150, 326
StyI CCWWGG 1 cut(s) 149
TaaI ACNGT 2 cut(s) 71, 124
TaiI ACGT 1 cut(s) 529
TaqI TCGA 1 cut(s) 207
TasI AATT 3 cut(s) 280, 392, 465
TatI WGTACW 1 cut(s) 303
TauI GCSGC 1 cut(s) 367
Tru1I TTAA 3 cut(s) 29, 450, 562
Tru9I TTAA 3 cut(s) 29, 450, 562
TscAI CASTG 4 cut(s) 351, 394, 430, 538
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 3 cut(s) 476, 486, 489
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 1 cut(s) 486
TspRI CASTG 4 cut(s) 351, 394, 430, 538
VneI GTGCAC 1 cut(s) 128
XapI RAATTY 1 cut(s) 392
XmaJI CCTAGG 1 cut(s) 149
XspI CTAG 2 cut(s) 150, 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.