RchiOBHm_Chr1g0370491

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002740
Physical Location & Seq
Forward (+)
60002594 .. 60013477
10884 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5106 bp
ATGGCGGCGGCTAACGCTCCGATCACCATGAAGGAGGCCCTAACGCTTCCGAGCGTTGGGATTAATCCGCAATTCATAACGTTTACACATGTTACTATGGAGTCCGATAAGTACATATGTGTTCGGGAAACAGCGCCGCAGAATAGTATTGTGATTATTGATATGAGTATGCCGAATCAACCTTTGAGGAGGCCTATCACCGCTGACTCTGCACTTATGAATCCAAACTCCAAAATCCTTGCTTTGAAAGCTCAAGTCCAGGGAACTACTCAGGACCACCTTCAAATTTTTAACATTGAGATGAAAGCAAAACTGAAATCACACCTTATGCCTGAGCAGATTGTCTTTTGGAAATGGATAACCCCAAAGATGTTGGGTCTTGTCACACAGACTACAGTATATCATTGGTCAATTGAAGGTGAGTCTGAACCAGTAAAGGTATTTGAGCGAACAGCTAACCTGGCAAACAATCAGATCATAAATTACCGTTGTGATCCTTCTGAGAAGTGGTTGGTTCTGATTGGAATTGCTCCTGGTGCTCCTGAGAGGCCGCAATTGGTTAAAGGGAACTTGCAACTCTTCTCGGTGGATCAGCAGCGCAGTCAAGCTCTTGAAGCGCATGCCGCATCCTTTGCCCAGTACAAGGTTCCAGGGAATGAGAATCCTTCCATTCTGATTTCCTTTGCCACTAAGACACTCAATGCTGGACAGATTACATCCAAGTTGCATGTTATTGAGCTTGGTGCCCAGCCAGGGAAGCCATCTTTTACAAAGAAACAAGCAGATCTTTTCTTTCCTCCAGATTTTGCAGATGATTTCCCAGTTGCCATGCAGATGTCTCACAAGTACAGTTTGATTTATGTAATTACAAAGCTGGGGCTTCTATTTGTATACGACTTAGAGACAGCTAGTGCAGTTTACAGAAATAGAATTAGTCCAGATCCAATTTTCTTGACGACAGAAGCTTCATCAGTGGGAGGGTTTTATGCTGTTAATAGGCGGGGCCAGGTGTTGTTGGCTACCATTAATGAGCAAACAATTGTGCCTTTTGTCAGTGGTCAGTTAAACAATTTGGAGCTTGCCGTCAATCTGGCAAAAAGAGGAAATCTTCCTGGTGCTGAGAATCTGGTTGTCCAGCGTTTCCAAGAACTGTTTGCCCAAACCAAGTACAAAGAAGCTGCCGAACTGGCTGCTGAGTCCCCACAAGGAATTCTTCGTACACCGGATACAGTTGCTAAATTTCAGAGTGTTCCTGTCCAAGCTGGGCAAACACCCCCTTTGTTGCAATACTTTGGGACTCTCTTAACGAGAGGGAAGCTCAATGCCTTTGAGTCATTGGAATTGTCACGTCTTGTTGTGAACCAGAACAAGAAGAATCTTTTGGAAAACTGGTTGGCAGAGGACAAACTGGAATGCAGTGAGGAACTAGGAGATCTTGTGAAGACTGTGGATAATGATCTGGCTTTGAAAATATACATTAAAGCCAGAGCAACTCCAAAAGTTGTTGCAGCTTTTGCCGAGCGAAGGGAGTTTGACAAAATCCTGATCTACTCAAAGCAGGTTGGCTACACACCTGACTATCTGTTCCTTTTGCAAACAATTCTCCGAGCAGACCCGCAGGGTGCTGTTAATTTTGCATTGATGATGTCCCAAATGGAGGGTGGTTGTCCTGTTGATTACAACACCATTACAGATCTCTTTCTTCAGAGGAATCTGATTCGTGAGGCAACCGCTTTTCTACTGGATGTTTTGAAGCCAAATCTCCCTGAACATGCCTTCCTGCAGACAAAGGTCCTCGAGATTAACTTGGTTACTTTTCCAAATGTGGCTGATGCCATTTTAGCCAATGGTATGTTCAGTCATTATGATCGTCCTCGTATTGCCCAGTTATGTGAAAAAGCTGGTCTTTATGTGCGAGCTTTGCAACACTACTCCGAGTTGCCTGATATTAAACGTGTCATAGTGAATACACATGCAATAGAGCCGCAGTCACTAGTAGAGTTTTTTGGTACCTTGTCAAAGGAATGGGCGTTGGAGTGTATGAAGGATCTCTTGCTGGTCAATTTGAGAGGCAACCTTCAGATAATTGTGCAGGTTGCTAAGGAGTACTCTGATCAGTTGGGTACTGATCAATGCATGAAGCTATTTGAGCAGTTCAAGTCATATGAAGGATTGTACTTCTTCCTCGGATCATTTTTGAGTTCAAGTGAGGATCCTGAAATCCACTTCAAGTACATTGAGGCAGCTGCCAAAACTGGACAAATTAAAGAGGTTGAGAGAGTTACTAGAGAATCAAATTTCTATGATGCAGAGAAAACAAAGAACTTTTTAATGGAAGCTAAGCTCCCTGATGCACGACCCCTTATCAATGTTTGCGACCGTTTTGGTTTTGTTCCAGATCTCACCCACTACTTGTACACAAACAATATGCTCCGTTACATTGAGGGTTACGTTCAAAAGGTGAATCCTTCAAATGCTCCTTTAGTTGTGGGACAGTTGCTAGATGACGAGTGCCCTGAAGATTTCATCAAAGGCTTGATCCTTTCTGTACGTTCATTGCTTCCAGTGGAGCCCCTTGTGGAGGAATGTGAGAAGAGGAATCGACTTCGTTTACTCACTCAGTTCTTGGAGCATCTTGTGAGTGAGGGAAGCCAGGATGTGCACGTCCACAATGCGTTGGGTAAAATCATCATTGATAGTGGAAACAATCCTGAACACTTCCTTACCACAAATCCATATTATGATTCTCGTGTTGTGGGTAAATATTGTGAGAAACGTGATCCTACTCTGGCTGTTGTTGCTTATCGACGTGGGCAATGTGATGATGAGCTTATCAATGTTACAAATAAAAACTCTTTGTTCAAATTACAAGCAAGATATGTCGTTGAGAGGATGGATGAGGATCTTTGGGCTAAAGTTCTTGACCCTGAGAATGAATTTAGAAGACAACTTATCGATCAAGTGGTTTCTACTGCCTTGCCTGAAAGCAAGAGCCCAGAACAAGTTTCTGCGGCTGTTAAGGCTTTCATGACTGCTGATTTGCCCCATGAATTAATTGAGCTTCTTGAAAAGATTGTGCTTCAAAACTCTGCCTTCAGTGGCAACTTTAATCTGCAAAATCTACTCATTTTGACAGCCATCAAGGCAGATCCGTCCAGAGTAATGGATTATATTAATAGGCTAGACAATTTCGATGGACCTGCAGTTGGGGAAGTAGCTGTGGAAGCCCAACTGTATGAGGAAGCATTTGCAATTTTCAAGAAGTTCAACTTGAATGTCCAAGCTGTCAATGTCTTGCTGGATAATATCCGAAGCATTGATAGGGCTGTAGAATTTGCATTCCGTGTTGAAGAGGATGCTGTTTGGAGTCAGGTTGGAAAGGCTCAACTCCGGGAGGGACTTGTGAGTGATGCGATTGAGTCATTTATTAGGGCGGATGATGCCACACAATTCTTGGATGTTATTCGAGCTTCTGAAGAAGCGGATGTTTACCATGACTTGGTGAGATACCTTCTGATGGTTAGGCAGAAGACAAAAGAGCCGAAGGTGGACAGTGAGCTCATTTATGCATATGCCAAGATTGATAGGCTGGCTGACATTGAAGAGTTCATTCTTATGCCAAATGTTGCCAATCTTCAAAATGTTGGTGATCGCCTATATGATGAGACCCTCTATGAGGCTGCAAAAATAATTTATGCTTTTATATCCAACTGGGCCAAGTTGGCTGTCACACTTGTCAAGCTCAAACAATTCCAAGGTGCTGTTGATGCAGCACGAAAAGCCAATAGTGCAAAGACATGGAAGGAAGTTTGCTTTGCTTGTGTTGATGCTGAGGAGTTCCGCTTAGCTCAAATTTGTGGTCTCAACATCATTATACAGGTGGATGATTTGGAAGAGGTCAGTGAATTCTACCAGAATAGAGGGTGCTTCAATGAGCTAATATCTCTAATGGAAAGTGGTCTTGGCTTAGAACGTGCACACATGGGAATCTTTACTGAATTGGGGGTTCTGTATGCTAGATACCGTCCTGAGAAACTTATGGAGCACATCAAACTGTTCTCAACTCGTTTAAATATTCCCAAGCTCATTCGAGCTTGTGATGAGCAGCAGCATTGGAAGGAGCTAACCTATCTATATATTCAATATGATGAGTTTGATAATGCTGCAACAACCATCATGAATCATTCCCCTGAAGCCTGGGATCACATGCAGTTCAAAGATGTGGCAGTTAAAGTTGCAAATGTGGAGCTATATTATAAGGCTGTGCACTTTTACTTGCAAGAGCATCCAGATCTCATTAATGATATTCTAAATGTGCTTGCTCTCCGTGTAGATCATACTCGTGTGGTGGACATTATGCGAAAGGCTGGGCACCTACTCCTTGTGAAGCCATACATGGTTGCTGTTCAGAGCAACAATGTCTCAGCTGTGAATGAGGCTTTGAATGAGATATATGTTGAAGAGGAAGACTATGAAAGATTGCGTGAATCAATTGATTTACATGACAGCTTTGATCAAATAGGTCTTGCTCAGAAGATTGAAAAACACGAGCTTCTGGAAATGAGGCGCGTTGCTGCTTATATTTACAAGAAAGCTGGAAGGTGGAAGCAGTCCATTGCATTATCAAAGAAAGACAAACTTTACAAAGATGCCATGGAGACAGCCTCACAGTCTGGTGACCGCGAACTTGCGGAGGAATTGCTTGTTTACTTCATTGAACAGGGAAAGAAAGAATGCTTTGCGTCATGCCTCTTCGTCTGTTACGATTTGATTAGGCCGGATACTGCCCTTGAACTTGCTTGGATGAACAATATGATAGACTTTGCCTTCCCATATCTGTTGCAGTTCATCCGTGAATACACCGGTAAAGTCGATGAATTGGTGAAGGACAGAATTGAAGCCCAGAATGAGGTCAAGGAGAAGGAGAAGGAAGAGAAAGAAGTTATAGCACAGCAGAACATGTATGCACAGTTGTTGCCTCTTGCTTTGCCTGCTCCACCAATGCCTGGTATGGGAGGAGGGTATGGTCCTCCGCCTCCAATGGGAGGAATGGGGATGCCTCCGATGCCACCTTATGGCATGCCACCAATGGGCAGTAGCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100
Pfam Domains
Protein Families
Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

1701

Amino Acids

192.92

Weight (kDa)

5.27

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_propel PF01394 20 - 55 2.9e-06 Clathrin propeller repeat
Clathrin-link PF09268 344 - 367 9.3e-09 Clathrin, heavy-chain linker
Clathrin_H_link PF13838 369 - 434 4e-28 Clathrin-H-link
Clathrin PF00637 557 - 692 7.9e-25 Region in Clathrin and VPS
Clathrin PF00637 701 - 841 7.4e-27 Region in Clathrin and VPS
Clathrin PF00637 850 - 984 1.6e-32 Region in Clathrin and VPS
Clathrin PF00637 993 - 1131 4.1e-32 Region in Clathrin and VPS
Clathrin PF00637 1144 - 1281 5.7e-31 Region in Clathrin and VPS
Clathrin PF00637 1290 - 1432 2.2e-27 Region in Clathrin and VPS
Clathrin PF00637 1438 - 1579 3.7e-32 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4257
Acc36I ACCTGC 3 cut(s) 1549, 2083, 3208
Acc65I GGTACC 1 cut(s) 2009
AccB1I GGYRCC 3 cut(s) 743, 2009, 4371
AccB7I CCANNNNNTGG 3 cut(s) 3499, 5006, 5075
AccI GTMKAC 1 cut(s) 891
AccII CGCG 2 cut(s) 4569, 4683
AclI AACGTT 1 cut(s) 80
AcsI RAATTY 8 cut(s) 285, 1209, 1238, 2296, 2936, 3332, 3852, 3905
AcuI CTGAAG 6 cut(s) 1688, 2063, 2538, 3079, 3495, 4212
AflIII ACRYGT 3 cut(s) 88, 1954, 4959
AgeI ACCGGT 1 cut(s) 4862
AhlI ACTAGT 1 cut(s) 1993
AjiI CACGTC 3 cut(s) 1349, 2665, 2810
AjuI GAANNNNNNNTTGG 6 cut(s) 1364, 1396, 1838, 1870, 3945, 3977
AloI GAACNNNNNNTCC 2 cut(s) 1761, 1793
Alw21I GWGCWC 6 cut(s) 541, 2664, 3561, 3979, 4047, 4269
Alw26I GTCTC 6 cut(s) 843, 896, 3659, 3866, 4426, 4652
Alw44I GTGCAC 3 cut(s) 2660, 3975, 4265
Ama87I CYCGRG 1 cut(s) 1796
AoxI GGCC 6 cut(s) 36, 191, 548, 1003, 3714, 4775
ApaLI GTGCAC 3 cut(s) 2660, 3975, 4265
ApoI RAATTY 8 cut(s) 285, 1209, 1238, 2296, 2936, 3332, 3852, 3905
ArsI GACNNNNNNTTYG 4 cut(s) 3022, 3054, 3844, 3876
AseI ATTAAT 5 cut(s) 63, 1026, 3053, 3174, 4299
AsiGI ACCGGT 1 cut(s) 4862
Asp718I GGTACC 1 cut(s) 2009
AspLEI GCGC 4 cut(s) 136, 600, 619, 4569
AspS9I GGNCC 7 cut(s) 37, 274, 1003, 1792, 3197, 3714, 5027
AsuC2I CCSGG 1 cut(s) 3392
AsuHPI GGTGA 9 cut(s) 16, 190, 431, 2395, 2473, 3514, 3659, 4688, 4894
AvaI CYCGRG 1 cut(s) 1796
AvaII GGWCC 4 cut(s) 274, 1792, 3197, 5027
BaeGI GKGCMC 6 cut(s) 748, 2516, 2664, 3979, 4269, 4374
BamHI GGATCC 1 cut(s) 2212
BanI GGYRCC 3 cut(s) 743, 2009, 4371
BanII GRGCYC 3 cut(s) 2574, 2996, 3561
BauI CACGAG 3 cut(s) 2748, 4341, 4547
BbsI GAAGAC 4 cut(s) 1448, 2950, 3536, 4473
Bbv12I GWGCWC 6 cut(s) 541, 2664, 3561, 3979, 4047, 4269
BbvCI CCTCAGC 1 cut(s) 3831
BccI CCATC 6 cut(s) 769, 2887, 3146, 3188, 3511, 4181
BceAI ACGGC 1 cut(s) 1067
BcgI CGANNNNNNTGC 4 cut(s) 1172, 1206, 3182, 3216
BciVI GTATCC 2 cut(s) 1219, 4774
BclI TGATCA 3 cut(s) 2113, 2128, 4513
BcnI CCSGG 1 cut(s) 3392
BcoDI GTCTC 6 cut(s) 843, 896, 3659, 3866, 4426, 4652
BcuI ACTAGT 1 cut(s) 1993
BfaI CTAG 7 cut(s) 909, 1427, 1994, 2286, 2501, 3182, 4017
BfmI CTRYAG 4 cut(s) 393, 1781, 3201, 3327
BfoI RGCGCY 1 cut(s) 137
BfuAI ACCTGC 3 cut(s) 1549, 2083, 3208
BfuI GTATCC 2 cut(s) 1219, 4774
BglI GCCNNNNNGGC 3 cut(s) 1187, 3143, 3722
BglII AGATCT 5 cut(s) 784, 1432, 1693, 2398, 4291
BlpI GCTNAGC 2 cut(s) 2340, 3844
BmcAI AGTACT 1 cut(s) 2108
Bme18I GGWCC 4 cut(s) 274, 1792, 3197, 5027
BmeT110I CYCGRG 1 cut(s) 1796
BmgBI CACGTC 3 cut(s) 1349, 2665, 2810
BmgT120I GGNCC 7 cut(s) 37, 274, 1003, 1792, 3197, 3714, 5027
BmiI GGNNCC 7 cut(s) 648, 745, 1004, 2011, 2214, 2571, 4373
BmrI ACTGGG 4 cut(s) 631, 815, 1879, 3721
BmuI ACTGGG 4 cut(s) 631, 815, 1879, 3721
BoxI GACNNNNGTC 1 cut(s) 1790
BpiI GAAGAC 4 cut(s) 1448, 2950, 3536, 4473
BplI GAGNNNNNCTC 4 cut(s) 1302, 1334, 4649, 4681
BpmI CTGGAG 1 cut(s) 783
Bpu10I CCTNAGC 3 cut(s) 333, 2100, 3831
Bpu1102I GCTNAGC 2 cut(s) 2340, 3844
BpuEI CTTGAG 1 cut(s) 237
BpuMI CCSGG 1 cut(s) 3392
Bsa29I ATCGAT 1 cut(s) 2955
BsaBI GATNNNNATC 2 cut(s) 1455, 2901
BsaI GGTCTC 2 cut(s) 3659, 3866
BsaJI CCNNGG 7 cut(s) 259, 650, 752, 2185, 3754, 4197, 4653
BsaWI WCCGGW 2 cut(s) 1222, 4862
BsaXI ACNNNNNCTCC 2 cut(s) 1422, 1452
Bse118I RCCGGY 1 cut(s) 4862
Bse3DI GCAATG 3 cut(s) 2555, 2822, 4614
Bse8I GATNNNNATC 2 cut(s) 1455, 2901
BseCI ATCGAT 1 cut(s) 2955
BseDI CCNNGG 7 cut(s) 259, 650, 752, 2185, 3754, 4197, 4653
BseJI GATNNNNATC 2 cut(s) 1455, 2901
BseMI GCAATG 3 cut(s) 2555, 2822, 4614
BseRI GAGGAG 3 cut(s) 202, 3848, 5031
BseSI GKGCMC 6 cut(s) 748, 2516, 2664, 3979, 4269, 4374
BseYI CCCAGC 4 cut(s) 747, 874, 1262, 4367
BsgI GTGCAG 3 cut(s) 195, 933, 2111
Bsh1236I CGCG 2 cut(s) 4569, 4683
Bsh1285I CGRYCG 1 cut(s) 2380
BshFI GGCC 6 cut(s) 38, 193, 550, 1005, 3716, 4777
BshNI GGYRCC 3 cut(s) 743, 2009, 4371
BshTI ACCGGT 1 cut(s) 4862
BshVI ATCGAT 1 cut(s) 2955
BsiEI CGRYCG 1 cut(s) 2380
BsiHKAI GWGCWC 6 cut(s) 541, 2664, 3561, 3979, 4047, 4269
BsiHKCI CYCGRG 1 cut(s) 1796
BsiSI CCGG 4 cut(s) 1223, 3391, 4778, 4863
BslFI GGGAC 5 cut(s) 1183, 1309, 1633, 2505, 3411
BsmAI GTCTC 6 cut(s) 843, 896, 3659, 3866, 4426, 4652
BsmFI GGGAC 5 cut(s) 1183, 1309, 1633, 2505, 3411
BsmI GAATGC 3 cut(s) 1418, 3338, 4739
BsnI GGCC 6 cut(s) 38, 193, 550, 1005, 3716, 4777
Bso31I GGTCTC 2 cut(s) 3659, 3866
BsoBI CYCGRG 1 cut(s) 1796
Bsp1407I TGTACA 1 cut(s) 2415
Bsp1720I GCTNAGC 2 cut(s) 2340, 3844
Bsp19I CCATGG 1 cut(s) 4653
BspANI GGCC 6 cut(s) 38, 193, 550, 1005, 3716, 4777
BspDI ATCGAT 1 cut(s) 2955
BspFNI CGCG 2 cut(s) 4569, 4683
BspHI TCATGA 2 cut(s) 3027, 4176
BspLI GGNNCC 7 cut(s) 648, 745, 1004, 2011, 2214, 2571, 4373
BspMAI CTGCAG 2 cut(s) 1785, 3205
BspMI ACCTGC 3 cut(s) 1549, 2083, 3208
BspT107I GGYRCC 3 cut(s) 743, 2009, 4371
BspTNI GGTCTC 2 cut(s) 3659, 3866
BsrDI GCAATG 3 cut(s) 2555, 2822, 4614
BsrFI RCCGGY 1 cut(s) 4862
BsrGI TGTACA 1 cut(s) 2415
BssAI RCCGGY 1 cut(s) 4862
BssECI CCNNGG 7 cut(s) 259, 650, 752, 2185, 3754, 4197, 4653
BssNAI GTATAC 1 cut(s) 892
BssSI CACGAG 3 cut(s) 2748, 4341, 4547
BssT1I CCWWGG 2 cut(s) 3754, 4653
Bst1107I GTATAC 1 cut(s) 892
Bst2BI CACGAG 3 cut(s) 2748, 4341, 4547
Bst6I CTCTTC 8 cut(s) 584, 2588, 3345, 3597, 3888, 4455, 4757, 4926
BstAPI GCANNNNNTGC 2 cut(s) 632, 1514
BstAUI TGTACA 1 cut(s) 2415
BstC8I GCNNGC 7 cut(s) 621, 1080, 1917, 3591, 4320, 4992, 5081
BstDSI CCRYGG 1 cut(s) 4653
BstEII GGTNACC 1 cut(s) 4676
BstENI CCTNNNNNAGG 2 cut(s) 2579, 3674
BstFNI CGCG 2 cut(s) 4569, 4683
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 4 cut(s) 136, 600, 619, 4569
BstMAI GTCTC 6 cut(s) 843, 896, 3659, 3866, 4426, 4652
BstMCI CGRYCG 1 cut(s) 2380
BstNSI RCATGY 8 cut(s) 92, 623, 731, 1775, 1976, 4210, 4963, 5083
BstPAI GACNNNNGTC 1 cut(s) 1790
BstPI GGTNACC 1 cut(s) 4676
BstSFI CTRYAG 4 cut(s) 393, 1781, 3201, 3327
BstSLI GKGCMC 6 cut(s) 748, 2516, 2664, 3979, 4269, 4374
BstUI CGCG 2 cut(s) 4569, 4683
BstV2I GAAGAC 4 cut(s) 1448, 2950, 3536, 4473
BstXI CCANNNNNNTGG 1 cut(s) 3163
BstZ17I GTATAC 1 cut(s) 892
Bsu15I ATCGAT 1 cut(s) 2955
BsuI GTATCC 2 cut(s) 1219, 4774
BsuRI GGCC 6 cut(s) 38, 193, 550, 1005, 3716, 4777
BsuTUI ATCGAT 1 cut(s) 2955
BtgI CCRYGG 1 cut(s) 4653
BtrI CACGTC 3 cut(s) 1349, 2665, 2810
BtsI GCAGTG 1 cut(s) 1423
BtsIMutI CAGTG 7 cut(s) 978, 1060, 1423, 2571, 3103, 3559, 3907
BveI ACCTGC 3 cut(s) 1549, 2083, 3208
Cac8I GCNNGC 7 cut(s) 621, 1080, 1917, 3591, 4320, 4992, 5081
CciI TCATGA 2 cut(s) 3027, 4176
CfoI GCGC 4 cut(s) 136, 600, 619, 4569
Cfr10I RCCGGY 1 cut(s) 4862
Cfr13I GGNCC 7 cut(s) 37, 274, 1003, 1792, 3197, 3714, 5027
ClaI ATCGAT 1 cut(s) 2955
CseI GACGC 1 cut(s) 4731
CspAI ACCGGT 1 cut(s) 4862
DraI TTTAAA 1 cut(s) 4071
Eam1104I CTCTTC 8 cut(s) 584, 2588, 3345, 3597, 3888, 4455, 4757, 4926
EarI CTCTTC 8 cut(s) 584, 2588, 3345, 3597, 3888, 4455, 4757, 4926
EciI GGCGGA 2 cut(s) 3449, 5022
Ecl136II GAGCTC 1 cut(s) 3559
Eco130I CCWWGG 2 cut(s) 3754, 4653
Eco147I AGGCCT 1 cut(s) 193
Eco24I GRGCYC 3 cut(s) 2574, 2996, 3561
Eco31I GGTCTC 2 cut(s) 3659, 3866
Eco47I GGWCC 4 cut(s) 274, 1792, 3197, 5027
Eco53kI GAGCTC 1 cut(s) 3559
Eco57I CTGAAG 6 cut(s) 1688, 2063, 2538, 3079, 3495, 4212
Eco88I CYCGRG 1 cut(s) 1796
Eco91I GGTNACC 1 cut(s) 4676
EcoICRI GAGCTC 1 cut(s) 3559
EcoNI CCTNNNNNAGG 2 cut(s) 2579, 3674
EcoO109I RGGNCCY 2 cut(s) 37, 1792
EcoO65I GGTNACC 1 cut(s) 4676
EcoRI GAATTC 2 cut(s) 1209, 3905
EcoT14I CCWWGG 2 cut(s) 3754, 4653
EcoT22I ATGCAT 2 cut(s) 2138, 3571
EcoT38I GRGCYC 3 cut(s) 2574, 2996, 3561
ErhI CCWWGG 2 cut(s) 3754, 4653
FaqI GGGAC 5 cut(s) 1183, 1309, 1633, 2505, 3411
FauI CCCGC 2 cut(s) 993, 1623
FauNDI CATATG 3 cut(s) 116, 2164, 3571
FbaI TGATCA 3 cut(s) 2113, 2128, 4513
FblI GTMKAC 1 cut(s) 891
FriOI GRGCYC 3 cut(s) 2574, 2996, 3561
FspBI CTAG 7 cut(s) 909, 1427, 1994, 2286, 2501, 3182, 4017
GlaI GCGC 4 cut(s) 135, 599, 618, 4568
GsaI CCCAGC 4 cut(s) 751, 878, 1266, 4371
GsuI CTGGAG 1 cut(s) 783
HaeII RGCGCY 1 cut(s) 137
HaeIII GGCC 6 cut(s) 38, 193, 550, 1005, 3716, 4777
HapII CCGG 4 cut(s) 1223, 3391, 4778, 4863
HgaI GACGC 1 cut(s) 4731
HhaI GCGC 4 cut(s) 136, 600, 619, 4569
Hin6I GCGC 4 cut(s) 134, 598, 617, 4567
HinP1I GCGC 4 cut(s) 134, 598, 617, 4567
HindIII AAGCTT 1 cut(s) 963
HpaII CCGG 4 cut(s) 1223, 3391, 4778, 4863
HphI GGTGA 9 cut(s) 16, 190, 431, 2395, 2473, 3514, 3659, 4688, 4894
Hpy99I CGWCG 1 cut(s) 2811
HpyCH4IV ACGT 9 cut(s) 80, 1348, 1954, 2451, 2551, 2664, 2776, 2809, 3973
HpySE526I ACGT 9 cut(s) 80, 1348, 1954, 2451, 2551, 2664, 2776, 2809, 3973
HspAI GCGC 4 cut(s) 134, 598, 617, 4567
KpnI GGTACC 1 cut(s) 2013
Ksp22I TGATCA 3 cut(s) 2113, 2128, 4513
MaeI CTAG 7 cut(s) 909, 1427, 1994, 2286, 2501, 3182, 4017
MaeII ACGT 9 cut(s) 80, 1348, 1954, 2451, 2551, 2664, 2776, 2809, 3973
MfeI CAATTG 4 cut(s) 411, 554, 1038, 4491
MlyI GAGTC 8 cut(s) 110, 200, 431, 1205, 1291, 1340, 3376, 3428
MmeI TCCRAC 3 cut(s) 2013, 3355, 3732
Mph1103I ATGCAT 2 cut(s) 2138, 3571
MslI CAYNNNNRTG 4 cut(s) 299, 833, 3614, 4341
MspA1I CMGCKG 3 cut(s) 203, 2246, 4427
MspI CCGG 4 cut(s) 1223, 3391, 4778, 4863
MunI CAATTG 4 cut(s) 411, 554, 1038, 4491
Mva1269I GAATGC 3 cut(s) 1418, 3338, 4739
MvnI CGCG 2 cut(s) 4569, 4683
NciI CCSGG 1 cut(s) 3392
NcoI CCATGG 1 cut(s) 4653
NdeI CATATG 3 cut(s) 116, 2164, 3571
NlaIV GGNNCC 7 cut(s) 648, 745, 1004, 2011, 2214, 2571, 4373
NmeAIII GCCGAG 1 cut(s) 1543
NmuCI GTSAC 5 cut(s) 382, 1344, 1989, 3727, 4676
NsiI ATGCAT 2 cut(s) 2138, 3571
NspI RCATGY 8 cut(s) 92, 623, 731, 1775, 1976, 4210, 4963, 5083
PaeI GCATGC 2 cut(s) 623, 5083
PaeR7I CTCGAG 1 cut(s) 1796
PagI TCATGA 2 cut(s) 3027, 4176
PceI AGGCCT 1 cut(s) 193
PciI ACATGT 2 cut(s) 88, 4959
PcsI WCGNNNNNNNCGW 1 cut(s) 4761
PctI GAATGC 3 cut(s) 1418, 3338, 4739
PflMI CCANNNNNTGG 3 cut(s) 3499, 5006, 5075
PfoI TCCNGGA 1 cut(s) 3390
PinAI ACCGGT 1 cut(s) 4862
PleI GAGTC 8 cut(s) 109, 200, 430, 1204, 1291, 1339, 3375, 3427
PpsI GAGTC 8 cut(s) 109, 200, 430, 1204, 1291, 1339, 3375, 3427
PpuMI RGGWCCY 1 cut(s) 1792
PscI ACATGT 2 cut(s) 88, 4959
PshAI GACNNNNGTC 1 cut(s) 1790
PshBI ATTAAT 5 cut(s) 63, 1026, 3053, 3174, 4299
PsiI TTATAA 1 cut(s) 4257
Psp124BI GAGCTC 1 cut(s) 3561
Psp1406I AACGTT 1 cut(s) 80
Psp5II RGGWCCY 1 cut(s) 1792
PspEI GGTNACC 1 cut(s) 4676
PspFI CCCAGC 4 cut(s) 747, 874, 1262, 4367
PspN4I GGNNCC 7 cut(s) 648, 745, 1004, 2011, 2214, 2571, 4373
PspPI GGNCC 7 cut(s) 37, 274, 1003, 1792, 3197, 3714, 5027
PspPPI RGGWCCY 1 cut(s) 1792
PstI CTGCAG 2 cut(s) 1785, 3205
PvuII CAGCTG 2 cut(s) 2246, 4427
RseI CAYNNNNRTG 4 cut(s) 299, 833, 3614, 4341
SacI GAGCTC 1 cut(s) 3561
Sau96I GGNCC 7 cut(s) 37, 274, 1003, 1792, 3197, 3714, 5027
ScaI AGTACT 1 cut(s) 2108
SchI GAGTC 8 cut(s) 110, 200, 431, 1205, 1291, 1340, 3376, 3428
SfcI CTRYAG 4 cut(s) 393, 1781, 3201, 3327
Sfr274I CTCGAG 1 cut(s) 1796
SinI GGWCC 4 cut(s) 274, 1792, 3197, 5027
SlaI CTCGAG 1 cut(s) 1796
SmiMI CAYNNNNRTG 4 cut(s) 299, 833, 3614, 4341
SmlI CTYRAG 2 cut(s) 252, 1796
SmoI CTYRAG 2 cut(s) 252, 1796
SpeI ACTAGT 1 cut(s) 1993
SphI GCATGC 2 cut(s) 623, 5083
SseBI AGGCCT 1 cut(s) 193
SspI AATATT 2 cut(s) 2765, 4075
SspMI CTAG 7 cut(s) 909, 1427, 1994, 2286, 2501, 3182, 4017
SstI GAGCTC 1 cut(s) 3561
StuI AGGCCT 1 cut(s) 193
StyI CCWWGG 2 cut(s) 3754, 4653
TaiI ACGT 9 cut(s) 83, 1351, 1957, 2454, 2554, 2667, 2779, 2812, 3976
TaqI TCGA 8 cut(s) 1797, 2602, 2806, 2955, 3192, 3466, 4090, 4872
TatI WGTACW 8 cut(s) 111, 639, 846, 1167, 2106, 2175, 2232, 2415
TauI GCSGC 7 cut(s) 8, 11, 139, 553, 626, 1987, 3014
TscAI CASTG 7 cut(s) 978, 1060, 1423, 2571, 3103, 3559, 3907
TseFI GTSAC 5 cut(s) 382, 1344, 1989, 3727, 4676
Tsp45I GTSAC 5 cut(s) 382, 1344, 1989, 3727, 4676
TspGWI ACGGA 5 cut(s) 2423, 3141, 3332, 4316, 4841
TspRI CASTG 7 cut(s) 978, 1060, 1423, 2571, 3103, 3559, 3907
Van91I CCANNNNNTGG 3 cut(s) 3499, 5006, 5075
VneI GTGCAC 3 cut(s) 2660, 3975, 4265
VpaK11BI GGWCC 4 cut(s) 274, 1792, 3197, 5027
VspI ATTAAT 5 cut(s) 63, 1026, 3053, 3174, 4299
XagI CCTNNNNNAGG 2 cut(s) 2579, 3674
XapI RAATTY 8 cut(s) 285, 1209, 1238, 2296, 2936, 3332, 3852, 3905
XceI RCATGY 8 cut(s) 92, 623, 731, 1775, 1976, 4210, 4963, 5083
XcmI CCANNNNNNNNNTGG 2 cut(s) 1658, 3451
XhoI CTCGAG 1 cut(s) 1796
XmiI GTMKAC 1 cut(s) 891
XspI CTAG 7 cut(s) 909, 1427, 1994, 2286, 2501, 3182, 4017
ZrmI AGTACT 1 cut(s) 2108
Zsp2I ATGCAT 2 cut(s) 2138, 3571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.