RchiOBHm_Chr1g0377601

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002024
Physical Location & Seq
Forward (+)
64411245 .. 64413809
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 717 bp
ATGCAGGAAAGGTGTTCATATATTAATTGGTTAGGGACAGAGCCATTTTTGTATATAGCAGATCCGGTTTTACTGAAAAAAATGTCGATAGAGGTCACTGCAAAGAATTGGGGGAAACCTACTGTGTTCAAACGTGATAGGGCTCCCATGTTTGGTAATGGTCTTGTCATGTCTGAAGGTGATGAATGGGCTCTTCACCGCCATGTTATCACTCCTGCGTTTAACGACTTTAACTTTAACCCTACTAACTTGAAGTCTAATGTACGTCCAATCCTATGGGTGAGCTTGATGGTGGAGACTACCACAATAATGCTAGACAAGTGGACTAACCAGCTCATGAATTCCAGCACTCAAGAAATTGATGTCGAGAAATCGATCACAGAAACAGCAGGGGAGATCATCGCCAAGGCCAGCTTCGGCATTAGTTACCGAAGTGACAGCCGAGTGTTTGTAAAATTCAGGGCCATACAAATCAGCCTCTTCCAAACAAAGCGCTTTCTGGGAGTTCCATTTGGGGAATTCTTGTACCCCAAGCAAGCCCTCGAGGCCAGAAGACTTGGAAAAGAAATTAACCAACTGTTCTCACCGCTCATAGCTTCACGAAAGAATTTGATTGGGTCAGTATTGTCTCCTGCGCAGCACGACCTGCTCGGATTATTACTTAACGAGAGTGATCAACAAGCAGGCAGCTTCAGAAAGGCTTTGACGACACAGTAG

Protein Analysis

238

Amino Acids

27.02

Weight (kDa)

9.32

Isoelectric Point (pI)

36.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 10 - 226 3.3e-11 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 542
Acc16I TGCGCA 1 cut(s) 636
Acc36I ACCTGC 1 cut(s) 654
AccBSI CCGCTC 1 cut(s) 589
AciI CCGC 2 cut(s) 199, 587
AclWI GGATC 1 cut(s) 56
AcsI RAATTY 4 cut(s) 340, 455, 518, 607
AcuI CTGAAG 2 cut(s) 195, 676
AfaI GTAC 2 cut(s) 264, 527
AfeI AGCGCT 1 cut(s) 494
AfiI CCNNNNNNNGG 1 cut(s) 152
AgsI TTSAA 2 cut(s) 130, 253
AjuI GAANNNNNNNTTGG 2 cut(s) 398, 430
AluBI AGCT 5 cut(s) 285, 334, 414, 596, 690
AluI AGCT 5 cut(s) 285, 334, 414, 596, 690
Alw26I GTCTC 2 cut(s) 290, 633
AlwI GGATC 1 cut(s) 56
Ama87I CYCGRG 1 cut(s) 542
Aor51HI AGCGCT 1 cut(s) 494
AoxI GGCC 3 cut(s) 408, 462, 546
ApeKI GCWGC 2 cut(s) 637, 687
ApoI RAATTY 4 cut(s) 340, 455, 518, 607
AseI ATTAAT 1 cut(s) 24
AspLEI GCGC 2 cut(s) 495, 637
AspS9I GGNCC 1 cut(s) 462
AsuHPI GGTGA 4 cut(s) 188, 191, 292, 576
AvaI CYCGRG 1 cut(s) 542
BanII GRGCYC 2 cut(s) 145, 193
BbsI GAAGAC 1 cut(s) 559
BbvI GCAGC 2 cut(s) 649, 699
BccI CCATC 1 cut(s) 283
BclI TGATCA 1 cut(s) 673
BcoDI GTCTC 2 cut(s) 290, 633
BfaI CTAG 1 cut(s) 314
BfoI RGCGCY 1 cut(s) 496
BfuAI ACCTGC 1 cut(s) 654
BglI GCCNNNNNGGC 1 cut(s) 545
BisI GCNGC 2 cut(s) 638, 688
BlsI GCNGC 2 cut(s) 639, 689
BmeT110I CYCGRG 1 cut(s) 542
BmgT120I GGNCC 1 cut(s) 462
BmiI GGNNCC 1 cut(s) 144
BpiI GAAGAC 1 cut(s) 559
BpuEI CTTGAG 1 cut(s) 336
Bsa29I ATCGAT 1 cut(s) 374
BsaJI CCNNGG 1 cut(s) 405
BsaWI WCCGGW 1 cut(s) 64
Bsc4I CCNNNNNNNGG 1 cut(s) 152
BseCI ATCGAT 1 cut(s) 374
BseDI CCNNGG 1 cut(s) 405
BseLI CCNNNNNNNGG 1 cut(s) 152
BseXI GCAGC 2 cut(s) 649, 699
BshFI GGCC 3 cut(s) 410, 464, 548
BshVI ATCGAT 1 cut(s) 374
BsiHKCI CYCGRG 1 cut(s) 542
BsiSI CCGG 1 cut(s) 65
BslFI GGGAC 1 cut(s) 49
BslI CCNNNNNNNGG 1 cut(s) 152
BsmAI GTCTC 2 cut(s) 290, 633
BsmFI GGGAC 1 cut(s) 49
BsnI GGCC 3 cut(s) 410, 464, 548
BsoBI CYCGRG 1 cut(s) 542
Bsp1286I GDGCHC 2 cut(s) 145, 193
Bsp143I GATC 4 cut(s) 61, 375, 396, 673
BspACI CCGC 2 cut(s) 199, 587
BspANI GGCC 3 cut(s) 410, 464, 548
BspDI ATCGAT 1 cut(s) 374
BspHI TCATGA 1 cut(s) 336
BspLI GGNNCC 1 cut(s) 144
BspMI ACCTGC 1 cut(s) 654
BspPI GGATC 1 cut(s) 56
BspQI GCTCTTC 1 cut(s) 198
BsrBI CCGCTC 1 cut(s) 589
BssECI CCNNGG 1 cut(s) 405
BssMI GATC 4 cut(s) 61, 375, 396, 673
BssT1I CCWWGG 1 cut(s) 405
Bst4CI ACNGT 3 cut(s) 124, 579, 714
Bst6I CTCTTC 2 cut(s) 198, 485
BstAPI GCANNNNNTGC 1 cut(s) 646
BstC8I GCNNGC 3 cut(s) 412, 537, 685
BstH2I RGCGCY 1 cut(s) 496
BstHHI GCGC 2 cut(s) 495, 637
BstKTI GATC 4 cut(s) 64, 378, 399, 676
BstMAI GTCTC 2 cut(s) 290, 633
BstMBI GATC 4 cut(s) 61, 375, 396, 673
BstMWI GCNNNNNNNGC 2 cut(s) 545, 646
BstV1I GCAGC 2 cut(s) 649, 699
BstV2I GAAGAC 1 cut(s) 559
BstX2I RGATCY 1 cut(s) 61
BstXI CCANNNNNNTGG 1 cut(s) 276
BstYI RGATCY 1 cut(s) 61
Bsu15I ATCGAT 1 cut(s) 374
BsuRI GGCC 3 cut(s) 410, 464, 548
BsuTUI ATCGAT 1 cut(s) 374
BtgZI GCGATG 1 cut(s) 385
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 1 cut(s) 96
BveI ACCTGC 1 cut(s) 654
Cac8I GCNNGC 3 cut(s) 412, 537, 685
CciI TCATGA 1 cut(s) 336
CfoI GCGC 2 cut(s) 495, 637
Cfr13I GGNCC 1 cut(s) 462
ClaI ATCGAT 1 cut(s) 374
Csp6I GTAC 2 cut(s) 263, 526
CviAII CATG 4 cut(s) 148, 169, 203, 337
CviQI GTAC 2 cut(s) 263, 526
DpnI GATC 4 cut(s) 63, 377, 398, 675
DpnII GATC 4 cut(s) 61, 375, 396, 673
Eam1104I CTCTTC 2 cut(s) 198, 485
EarI CTCTTC 2 cut(s) 198, 485
Eco130I CCWWGG 1 cut(s) 405
Eco24I GRGCYC 2 cut(s) 145, 193
Eco47III AGCGCT 1 cut(s) 494
Eco57I CTGAAG 2 cut(s) 195, 676
Eco88I CYCGRG 1 cut(s) 542
EcoRI GAATTC 2 cut(s) 340, 518
EcoT14I CCWWGG 1 cut(s) 405
EcoT38I GRGCYC 2 cut(s) 145, 193
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 4 cut(s) 151, 172, 206, 340
FalI AAGNNNNNCTT 2 cut(s) 398, 430
FaqI GGGAC 1 cut(s) 49
FatI CATG 4 cut(s) 147, 168, 202, 336
FbaI TGATCA 1 cut(s) 673
Fnu4HI GCNGC 2 cut(s) 638, 688
FriOI GRGCYC 2 cut(s) 145, 193
Fsp4HI GCNGC 2 cut(s) 638, 688
FspBI CTAG 1 cut(s) 314
FspI TGCGCA 1 cut(s) 636
GlaI GCGC 2 cut(s) 494, 636
GluI GCNGC 2 cut(s) 638, 688
HaeII RGCGCY 1 cut(s) 496
HaeIII GGCC 3 cut(s) 410, 464, 548
HapII CCGG 1 cut(s) 65
HhaI GCGC 2 cut(s) 495, 637
Hin1II CATG 4 cut(s) 151, 172, 206, 340
Hin6I GCGC 2 cut(s) 493, 635
HinP1I GCGC 2 cut(s) 493, 635
HpaII CCGG 1 cut(s) 65
HphI GGTGA 4 cut(s) 188, 191, 292, 576
Hpy166II GTNNAC 1 cut(s) 324
Hpy188I TCNGA 3 cut(s) 175, 653, 695
Hpy188III TCNNGA 4 cut(s) 337, 353, 367, 600
Hpy8I GTNNAC 1 cut(s) 324
HpyAV CCTTC 1 cut(s) 170
HpyCH4III ACNGT 3 cut(s) 124, 579, 714
HpyCH4IV ACGT 2 cut(s) 133, 265
HpyCH4V TGCA 2 cut(s) 4, 101
HpyF10VI GCNNNNNNNGC 2 cut(s) 545, 646
HpySE526I ACGT 2 cut(s) 133, 265
Hsp92II CATG 4 cut(s) 151, 172, 206, 340
HspAI GCGC 2 cut(s) 493, 635
Ksp22I TGATCA 1 cut(s) 673
Kzo9I GATC 4 cut(s) 61, 375, 396, 673
LguI GCTCTTC 1 cut(s) 198
LmnI GCTCC 1 cut(s) 148
Lsp1109I GCAGC 2 cut(s) 649, 699
MaeI CTAG 1 cut(s) 314
MaeII ACGT 2 cut(s) 133, 265
MaeIII GTNAC 3 cut(s) 94, 425, 434
MalI GATC 4 cut(s) 63, 377, 398, 675
MbiI CCGCTC 1 cut(s) 589
MboI GATC 4 cut(s) 61, 375, 396, 673
MboII GAAGA 3 cut(s) 185, 472, 564
MflI RGATCY 1 cut(s) 61
MhlI GDGCHC 2 cut(s) 145, 193
MluCI AATT 8 cut(s) 25, 106, 340, 357, 455, 518, 567, 607
MnlI CCTC 4 cut(s) 85, 488, 538, 551
MseI TTAA 6 cut(s) 24, 222, 231, 237, 570, 663
MslI CAYNNNNRTG 2 cut(s) 201, 308
MspI CCGG 1 cut(s) 65
MwoI GCNNNNNNNGC 2 cut(s) 545, 646
NdeII GATC 4 cut(s) 61, 375, 396, 673
NlaIII CATG 4 cut(s) 151, 172, 206, 340
NlaIV GGNNCC 1 cut(s) 144
NmeAIII GCCGAG 1 cut(s) 467
NmuCI GTSAC 2 cut(s) 94, 434
NsbI TGCGCA 1 cut(s) 636
PaeR7I CTCGAG 1 cut(s) 542
PagI TCATGA 1 cut(s) 336
PciSI GCTCTTC 1 cut(s) 198
PkrI GCNGC 2 cut(s) 639, 689
PshBI ATTAAT 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 144
PspPI GGNCC 1 cut(s) 462
PspXI VCTCGAGB 1 cut(s) 542
PsuI RGATCY 1 cut(s) 61
RsaI GTAC 2 cut(s) 264, 527
RsaNI GTAC 2 cut(s) 263, 526
RseI CAYNNNNRTG 2 cut(s) 201, 308
SapI GCTCTTC 1 cut(s) 198
SaqAI TTAA 6 cut(s) 24, 222, 231, 237, 570, 663
SatI GCNGC 2 cut(s) 638, 688
Sau3AI GATC 4 cut(s) 61, 375, 396, 673
Sau96I GGNCC 1 cut(s) 462
SduI GDGCHC 2 cut(s) 145, 193
Sfr274I CTCGAG 1 cut(s) 542
SlaI CTCGAG 1 cut(s) 542
SmiMI CAYNNNNRTG 2 cut(s) 201, 308
SmlI CTYRAG 2 cut(s) 351, 542
SmoI CTYRAG 2 cut(s) 351, 542
Sse9I AATT 8 cut(s) 25, 106, 340, 357, 455, 518, 567, 607
SsiI CCGC 2 cut(s) 199, 587
SspMI CTAG 1 cut(s) 314
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 3 cut(s) 124, 579, 714
TaiI ACGT 2 cut(s) 136, 268
TaqI TCGA 4 cut(s) 86, 366, 374, 543
TasI AATT 8 cut(s) 25, 106, 340, 357, 455, 518, 567, 607
Tru1I TTAA 6 cut(s) 24, 222, 231, 237, 570, 663
Tru9I TTAA 6 cut(s) 24, 222, 231, 237, 570, 663
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 2 cut(s) 94, 434
TseI GCWGC 2 cut(s) 637, 687
Tsp45I GTSAC 2 cut(s) 94, 434
TspDTI ATGAA 3 cut(s) 6, 198, 353
TspRI CASTG 1 cut(s) 103
VspI ATTAAT 1 cut(s) 24
XapI RAATTY 4 cut(s) 340, 455, 518, 607
XhoI CTCGAG 1 cut(s) 542
XspI CTAG 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.