RchiOBHm_Chr1g0377591

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002024
Physical Location & Seq
Forward (+)
64406573 .. 64409285
2713 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60103

Sequence Viewer

Length: 1413 bp
ATGGAGCACATGGCCATGGCAGTGATATTCTTCTTCTTCATCATTCTCTACTTATTATGCAGGCTGTCCCTTTCTTGTTGGGTTTTTCCTTTCCTAACTCACCGGAGGCTCAAGAAAAATGGTTTGACCGGCCCATCTCCAAGTTTTCCATTAGGAAACCTCAGTGAGATGAAGAAGAAGACTAACACTGGAAGCTCTTCTTGCACTAATAGTATATCCCATGACATATACTCAACTCTATTCCCTCACTTTGCTCGTTGGAGGAATTCCCACGGCAAGGTGTTCGTATACTGGTTGGGAACAGAGCCATTTTTGTACATAGCAGATCCTGTTTTAGTGAAAAAACTATCAACTCAGGTCTCTGCAAAGAACTGGGGCAAACCTACTGTGTTCAAACGCGACAGAGCTCCCATGTTTGGCAATGGCCTCATTATGTCTGATTGTCTGAAGGCAATGGTGAGTTTGATGGTGGAGACTACCAGAACAATGCTAGACACCTGGACTACCCAGCTCAGAAATTCCGACACTCGAGAAATTGATGTAGAGAAATCAATCAGAGAAACAGCAGGGGAGATCATCGCCAAGGCCAGCTTTGGCATTAGTTACCAAAGTGATCACCATGTGTTTGAAAAATTTAGGGCCTTGCAAATCAGCCTCTTCCAAACAAAACGCCTTTTGGGAGTTCCATTTGGGAAATTCTTGTACCTCAAGCAAGCCCTCGAGGCCAGCAGACTTGGAAAAGAAATTAACCAACTTGATCAACAAGGCAGCTTCAGAAAGGCTTTGACAACACAGCAGTTGGTGGATGAGTGCAAGACATTTTTCTTTTCAGGCCATGAGACATCGGCATTGTCTATCATATGGACGATGCTCCTTCTGGCCACGAATCCGGAGTGGCAAAATGAACTGAGGGAAGAGATTAGGGAGGTGGTAGGAGATAATAAAGAACTTCATTTCAACATGCTTGCTGGACTAAAGAAGATGGGATGGGTCATGAATGAGGTGTTCCGATTATATCCTTCAGGACCAAATGCACAGAGGCAAGCTAAAGATGACATTCGAGTGAGTGAAGACCTAACAATCCCTAGAGGAACCAATTTGTGGATTGACATTGTGGGCATGCACCATGACTCAGAGCTATGGGGTGAGGATGTGCATGAGTTCAAGCCAGAGAGGTTTAAGGATGATATTAATGGTGGGTGCAAGCACAAAATGGGTTTCTTACCTTTTGGGTTTGGAGGAAGGATGTGCATTGGTAGAAACTTGAGCTTTTTGGAGTATAAGATTGTGCTGACCCTAATTCTGTCTAGGTTTTCTTTTACCATGTCTCCTACATATTGTCACTGCCCTTCAACTTTACTCACTATGAGGCCATCTTATGGTCTGCCTCTAGTATTCCAGCCCTTGGACTGA

Protein Analysis

470

Amino Acids

53.91

Weight (kDa)

9.06

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 256 - 451 7.8e-54 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 719
AccB7I CCANNNNNTGG 3 cut(s) 1101, 1379, 1405
AccI GTMKAC 1 cut(s) 288
AccII CGCG 1 cut(s) 399
AccIII TCCGGA 1 cut(s) 889
AclWI GGATC 1 cut(s) 320
AcoI YGGCCR 2 cut(s) 12, 879
AcsI RAATTY 4 cut(s) 265, 517, 632, 695
AcuI CTGAAG 3 cut(s) 467, 757, 1005
AdeI CACNNNGTG 1 cut(s) 622
AfaI GTAC 2 cut(s) 317, 704
AfiI CCNNNNNNNGG 5 cut(s) 277, 416, 1101, 1379, 1405
AgsI TTSAA 5 cut(s) 394, 629, 958, 1165, 1353
AjnI CCWGG 1 cut(s) 497
AluBI AGCT 8 cut(s) 195, 407, 511, 591, 771, 1046, 1138, 1269
AluI AGCT 8 cut(s) 195, 407, 511, 591, 771, 1046, 1138, 1269
Alw21I GWGCWC 2 cut(s) 9, 409
Alw26I GTCTC 4 cut(s) 364, 467, 833, 1332
AlwI GGATC 1 cut(s) 320
AlwNI CAGNNNCTG 1 cut(s) 329
Ama87I CYCGRG 2 cut(s) 528, 719
Aor13HI TCCGGA 1 cut(s) 889
AoxI GGCC 9 cut(s) 12, 130, 424, 585, 639, 723, 832, 879, 1370
ApeKI GCWGC 1 cut(s) 768
ApoI RAATTY 4 cut(s) 265, 517, 632, 695
AseI ATTAAT 1 cut(s) 1191
Asp700I GAANNNNTTC 1 cut(s) 196
AspS9I GGNCC 3 cut(s) 131, 639, 1025
AsuHPI GGTGA 4 cut(s) 92, 469, 608, 1157
AvaI CYCGRG 2 cut(s) 528, 719
AvaII GGWCC 1 cut(s) 1025
BalI TGGCCA 2 cut(s) 14, 881
BanII GRGCYC 1 cut(s) 409
BbsI GAAGAC 2 cut(s) 185, 1077
Bbv12I GWGCWC 2 cut(s) 9, 409
BbvI GCAGC 1 cut(s) 780
BccI CCATC 5 cut(s) 142, 460, 976, 981, 1381
BceAI ACGGC 1 cut(s) 289
BcgI CGANNNNNNTGC 2 cut(s) 265, 299
BciT130I CCWGG 1 cut(s) 499
BclI TGATCA 2 cut(s) 613, 757
BcoDI GTCTC 4 cut(s) 364, 467, 833, 1332
BfaI CTAG 4 cut(s) 491, 1086, 1308, 1391
BglI GCCNNNNNGGC 1 cut(s) 722
BisI GCNGC 1 cut(s) 769
BlsI GCNGC 1 cut(s) 770
Bme1390I CCNGG 1 cut(s) 499
Bme18I GGWCC 1 cut(s) 1025
BmeT110I CYCGRG 2 cut(s) 528, 719
BmgT120I GGNCC 3 cut(s) 131, 639, 1025
BmiI GGNNCC 1 cut(s) 1093
BmrFI CCNGG 1 cut(s) 499
BmrI ACTGGG 1 cut(s) 382
BmsI GCATC 1 cut(s) 858
BmuI ACTGGG 1 cut(s) 382
BpiI GAAGAC 2 cut(s) 185, 1077
BpuEI CTTGAG 3 cut(s) 95, 692, 1285
BsaI GGTCTC 1 cut(s) 364
BsaJI CCNNGG 4 cut(s) 15, 271, 582, 1404
BsaWI WCCGGW 2 cut(s) 102, 889
BsaXI ACNNNNNCTCC 2 cut(s) 1312, 1342
Bsc4I CCNNNNNNNGG 5 cut(s) 277, 416, 1101, 1379, 1405
Bse118I RCCGGY 1 cut(s) 128
Bse1I ACTGG 3 cut(s) 193, 296, 377
Bse3DI GCAATG 2 cut(s) 427, 459
BseAI TCCGGA 1 cut(s) 889
BseBI CCWGG 1 cut(s) 499
BseDI CCNNGG 4 cut(s) 15, 271, 582, 1404
BseGI GGATG 5 cut(s) 811, 992, 1156, 1189, 1251
BseLI CCNNNNNNNGG 5 cut(s) 277, 416, 1101, 1379, 1405
BseMI GCAATG 2 cut(s) 427, 459
BseMII CTCAG 5 cut(s) 175, 368, 526, 899, 1146
BseNI ACTGG 3 cut(s) 193, 296, 377
BseXI GCAGC 1 cut(s) 780
BseYI CCCAGC 1 cut(s) 507
Bsh1236I CGCG 1 cut(s) 399
BshFI GGCC 9 cut(s) 14, 132, 426, 587, 641, 725, 834, 881, 1372
BsiHKAI GWGCWC 2 cut(s) 9, 409
BsiHKCI CYCGRG 2 cut(s) 528, 719
BsiSI CCGG 3 cut(s) 103, 129, 890
BslFI GGGAC 1 cut(s) 52
BslI CCNNNNNNNGG 5 cut(s) 277, 416, 1101, 1379, 1405
BsmAI GTCTC 4 cut(s) 364, 467, 833, 1332
BsmFI GGGAC 1 cut(s) 52
BsnI GGCC 9 cut(s) 14, 132, 426, 587, 641, 725, 834, 881, 1372
Bso31I GGTCTC 1 cut(s) 364
BsoBI CYCGRG 2 cut(s) 528, 719
Bsp1286I GDGCHC 2 cut(s) 9, 409
Bsp13I TCCGGA 1 cut(s) 889
Bsp1407I TGTACA 1 cut(s) 315
Bsp143I GATC 4 cut(s) 325, 573, 613, 757
Bsp19I CCATGG 1 cut(s) 15
BspANI GGCC 9 cut(s) 14, 132, 426, 587, 641, 725, 834, 881, 1372
BspCNI CTCAG 5 cut(s) 174, 367, 525, 900, 1145
BspEI TCCGGA 1 cut(s) 889
BspFNI CGCG 1 cut(s) 399
BspHI TCATGA 1 cut(s) 993
BspLI GGNNCC 1 cut(s) 1093
BspPI GGATC 1 cut(s) 320
BspQI GCTCTTC 1 cut(s) 202
BspTNI GGTCTC 1 cut(s) 364
BsrDI GCAATG 2 cut(s) 427, 459
BsrFI RCCGGY 1 cut(s) 128
BsrGI TGTACA 1 cut(s) 315
BsrI ACTGG 3 cut(s) 193, 296, 377
BssAI RCCGGY 1 cut(s) 128
BssECI CCNNGG 4 cut(s) 15, 271, 582, 1404
BssMI GATC 4 cut(s) 325, 573, 613, 757
BssNAI GTATAC 1 cut(s) 289
BssT1I CCWWGG 3 cut(s) 15, 582, 1404
Bst1107I GTATAC 1 cut(s) 289
Bst2UI CCWGG 1 cut(s) 499
Bst4CI ACNGT 1 cut(s) 388
Bst6I CTCTTC 3 cut(s) 202, 662, 909
BstAUI TGTACA 1 cut(s) 315
BstC8I GCNNGC 8 cut(s) 62, 589, 714, 727, 966, 1044, 1121, 1205
BstDEI CTNAG 5 cut(s) 161, 354, 512, 908, 1132
BstDSI CCRYGG 2 cut(s) 15, 271
BstF5I GGATG 5 cut(s) 811, 992, 1156, 1189, 1251
BstFNI CGCG 1 cut(s) 399
BstKTI GATC 4 cut(s) 328, 576, 616, 760
BstMAI GTCTC 4 cut(s) 364, 467, 833, 1332
BstMBI GATC 4 cut(s) 325, 573, 613, 757
BstMWI GCNNNNNNNGC 2 cut(s) 201, 722
BstNI CCWGG 1 cut(s) 499
BstNSI RCATGY 2 cut(s) 964, 1123
BstSCI CCNGG 1 cut(s) 497
BstUI CGCG 1 cut(s) 399
BstV1I GCAGC 1 cut(s) 780
BstV2I GAAGAC 2 cut(s) 185, 1077
BstX2I RGATCY 1 cut(s) 325
BstYI RGATCY 1 cut(s) 325
BstZ17I GTATAC 1 cut(s) 289
BsuRI GGCC 9 cut(s) 14, 132, 426, 587, 641, 725, 834, 881, 1372
BtgI CCRYGG 2 cut(s) 15, 271
BtgZI GCGATG 1 cut(s) 562
BtsCI GGATG 5 cut(s) 811, 992, 1156, 1189, 1251
BtsI GCAGTG 2 cut(s) 27, 1342
BtsIMutI CAGTG 4 cut(s) 27, 169, 186, 1342
Cac8I GCNNGC 8 cut(s) 62, 589, 714, 727, 966, 1044, 1121, 1205
CaiI CAGNNNCTG 1 cut(s) 329
CciI TCATGA 1 cut(s) 993
Cfr10I RCCGGY 1 cut(s) 128
Cfr13I GGNCC 3 cut(s) 131, 639, 1025
Csp6I GTAC 2 cut(s) 316, 703
CviQI GTAC 2 cut(s) 316, 703
DdeI CTNAG 5 cut(s) 161, 354, 512, 908, 1132
DpnI GATC 4 cut(s) 327, 575, 615, 759
DpnII GATC 4 cut(s) 325, 573, 613, 757
DraIII CACNNNGTG 1 cut(s) 622
EaeI YGGCCR 2 cut(s) 12, 879
Eam1104I CTCTTC 3 cut(s) 202, 662, 909
EarI CTCTTC 3 cut(s) 202, 662, 909
Ecl136II GAGCTC 1 cut(s) 407
Eco130I CCWWGG 3 cut(s) 15, 582, 1404
Eco24I GRGCYC 1 cut(s) 409
Eco31I GGTCTC 1 cut(s) 364
Eco47I GGWCC 1 cut(s) 1025
Eco53kI GAGCTC 1 cut(s) 407
Eco57I CTGAAG 3 cut(s) 467, 757, 1005
Eco88I CYCGRG 2 cut(s) 528, 719
EcoICRI GAGCTC 1 cut(s) 407
EcoO109I RGGNCCY 1 cut(s) 639
EcoRI GAATTC 1 cut(s) 265
EcoRII CCWGG 1 cut(s) 497
EcoT14I CCWWGG 3 cut(s) 15, 582, 1404
EcoT38I GRGCYC 1 cut(s) 409
ErhI CCWWGG 3 cut(s) 15, 582, 1404
FalI AAGNNNNNCTT 4 cut(s) 184, 216, 575, 607
FaqI GGGAC 1 cut(s) 52
FauNDI CATATG 1 cut(s) 860
FbaI TGATCA 2 cut(s) 613, 757
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 1 cut(s) 769
FokI GGATG 5 cut(s) 818, 999, 1163, 1196, 1258
FriOI GRGCYC 1 cut(s) 409
Fsp4HI GCNGC 1 cut(s) 769
FspBI CTAG 4 cut(s) 491, 1086, 1308, 1391
GluI GCNGC 1 cut(s) 769
GsaI CCCAGC 1 cut(s) 511
HaeIII GGCC 9 cut(s) 14, 132, 426, 587, 641, 725, 834, 881, 1372
HapII CCGG 3 cut(s) 103, 129, 890
HinfI GANTC 2 cut(s) 886, 1130
HpaII CCGG 3 cut(s) 103, 129, 890
HphI GGTGA 4 cut(s) 92, 469, 608, 1157
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 8 cut(s) 439, 447, 515, 523, 557, 776, 1010, 1135
Hpy188III TCNNGA 5 cut(s) 112, 530, 890, 994, 1023
Hpy8I GTNNAC 1 cut(s) 289
HpyAV CCTTC 5 cut(s) 442, 884, 1029, 1236, 1359
HpyCH4III ACNGT 1 cut(s) 388
HpyF10VI GCNNNNNNNGC 2 cut(s) 201, 722
HpyF3I CTNAG 5 cut(s) 161, 354, 512, 908, 1132
Kpn2I TCCGGA 1 cut(s) 889
Ksp22I TGATCA 2 cut(s) 613, 757
Kzo9I GATC 4 cut(s) 325, 573, 613, 757
LguI GCTCTTC 1 cut(s) 202
LmnI GCTCC 3 cut(s) 4, 412, 876
Lsp1109I GCAGC 1 cut(s) 780
LweI GCATC 1 cut(s) 858
MaeI CTAG 4 cut(s) 491, 1086, 1308, 1391
MaeIII GTNAC 2 cut(s) 602, 1340
MalI GATC 4 cut(s) 327, 575, 615, 759
MboI GATC 4 cut(s) 325, 573, 613, 757
MflI RGATCY 1 cut(s) 325
MhlI GDGCHC 2 cut(s) 9, 409
MlsI TGGCCA 2 cut(s) 14, 881
MluCI AATT 8 cut(s) 265, 517, 534, 632, 695, 744, 1096, 1299
MluNI TGGCCA 2 cut(s) 14, 881
MlyI GAGTC 1 cut(s) 1124
MmeI TCCRAC 2 cut(s) 239, 546
Mox20I TGGCCA 2 cut(s) 14, 881
MroI TCCGGA 1 cut(s) 889
MroXI GAANNNNTTC 1 cut(s) 196
MscI TGGCCA 2 cut(s) 14, 881
MseI TTAA 3 cut(s) 747, 1179, 1191
MslI CAYNNNNRTG 3 cut(s) 14, 20, 1061
Msp20I TGGCCA 2 cut(s) 14, 881
MspI CCGG 3 cut(s) 103, 129, 890
MspR9I CCNGG 1 cut(s) 499
MvaI CCWGG 1 cut(s) 499
MvnI CGCG 1 cut(s) 399
MwoI GCNNNNNNNGC 2 cut(s) 201, 722
NcoI CCATGG 1 cut(s) 15
NdeI CATATG 1 cut(s) 860
NdeII GATC 4 cut(s) 325, 573, 613, 757
NlaIV GGNNCC 1 cut(s) 1093
NmuCI GTSAC 1 cut(s) 1340
NspI RCATGY 2 cut(s) 964, 1123
PaeI GCATGC 1 cut(s) 1123
PaeR7I CTCGAG 2 cut(s) 528, 719
PagI TCATGA 1 cut(s) 993
PciSI GCTCTTC 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 196
PfeI GAWTC 1 cut(s) 886
PflMI CCANNNNNTGG 3 cut(s) 1101, 1379, 1405
PkrI GCNGC 1 cut(s) 770
PleI GAGTC 1 cut(s) 1124
PpsI GAGTC 1 cut(s) 1124
PshBI ATTAAT 1 cut(s) 1191
Psp124BI GAGCTC 1 cut(s) 409
Psp6I CCWGG 1 cut(s) 497
PspFI CCCAGC 1 cut(s) 507
PspGI CCWGG 1 cut(s) 497
PspN4I GGNNCC 1 cut(s) 1093
PspPI GGNCC 3 cut(s) 131, 639, 1025
PspXI VCTCGAGB 1 cut(s) 719
PstNI CAGNNNCTG 1 cut(s) 329
PsuI RGATCY 1 cut(s) 325
RsaI GTAC 2 cut(s) 317, 704
RsaNI GTAC 2 cut(s) 316, 703
RseI CAYNNNNRTG 3 cut(s) 14, 20, 1061
SacI GAGCTC 1 cut(s) 409
SapI GCTCTTC 1 cut(s) 202
SaqAI TTAA 3 cut(s) 747, 1179, 1191
SatI GCNGC 1 cut(s) 769
Sau3AI GATC 4 cut(s) 325, 573, 613, 757
Sau96I GGNCC 3 cut(s) 131, 639, 1025
SchI GAGTC 1 cut(s) 1124
ScrFI CCNGG 1 cut(s) 499
SduI GDGCHC 2 cut(s) 9, 409
SfaNI GCATC 1 cut(s) 858
Sfr274I CTCGAG 2 cut(s) 528, 719
SinI GGWCC 1 cut(s) 1025
SlaI CTCGAG 2 cut(s) 528, 719
SmiMI CAYNNNNRTG 3 cut(s) 14, 20, 1061
SmlI CTYRAG 5 cut(s) 110, 528, 707, 719, 1264
SmoI CTYRAG 5 cut(s) 110, 528, 707, 719, 1264
SphI GCATGC 1 cut(s) 1123
Sse9I AATT 8 cut(s) 265, 517, 534, 632, 695, 744, 1096, 1299
SspMI CTAG 4 cut(s) 491, 1086, 1308, 1391
SstI GAGCTC 1 cut(s) 409
StyD4I CCNGG 1 cut(s) 497
StyI CCWWGG 3 cut(s) 15, 582, 1404
TaaI ACNGT 1 cut(s) 388
TaqI TCGA 3 cut(s) 529, 720, 1060
TasI AATT 8 cut(s) 265, 517, 534, 632, 695, 744, 1096, 1299
TatI WGTACW 1 cut(s) 315
TfiI GAWTC 1 cut(s) 886
Tru1I TTAA 3 cut(s) 747, 1179, 1191
Tru9I TTAA 3 cut(s) 747, 1179, 1191
TscAI CASTG 4 cut(s) 27, 169, 193, 1349
TseFI GTSAC 1 cut(s) 1340
TseI GCWGC 1 cut(s) 768
Tsp45I GTSAC 1 cut(s) 1340
TspDTI ATGAA 5 cut(s) 28, 185, 918, 941, 1010
TspRI CASTG 4 cut(s) 27, 169, 193, 1349
Van91I CCANNNNNTGG 3 cut(s) 1101, 1379, 1405
VpaK11BI GGWCC 1 cut(s) 1025
VspI ATTAAT 1 cut(s) 1191
XapI RAATTY 4 cut(s) 265, 517, 632, 695
XceI RCATGY 2 cut(s) 964, 1123
XhoI CTCGAG 2 cut(s) 528, 719
XmiI GTMKAC 1 cut(s) 288
XmnI GAANNNNTTC 1 cut(s) 196
XspI CTAG 4 cut(s) 491, 1086, 1308, 1391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.