RchiOBHm_Chr0c32g0501491

Acylpyruvase FAHD1

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_chinensis
Unknown
OG0002365
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 168 bp
ATGAGGTGGAGGCGTGGAGCTGCTGTCATCATATCCAAGAAAGCTCGCGATGTTCCTCAGGCCAAGGCCATGGATTACGTTGGTGGTTATGCACTTGCTCTTGATATGACCGACAGAGAGATTCAAGCTACTGCTAAGGTAATCATCATAATTTGTAGTGTGAATTGA

Protein Analysis

55

Amino Acids

6.08

Weight (kDa)

9.74

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAA_hydrolase PF01557 8 - 46 8.4e-10 Fumarylacetoacetate (FAA) hydrolase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 48
AgsI TTSAA 1 cut(s) 125
AluBI AGCT 3 cut(s) 20, 44, 128
AluI AGCT 3 cut(s) 20, 44, 128
AoxI GGCC 2 cut(s) 60, 66
ApeKI GCWGC 1 cut(s) 20
AxyI CCTNAGG 1 cut(s) 57
BbvI GCAGC 1 cut(s) 7
BisI GCNGC 1 cut(s) 21
BlsI GCNGC 1 cut(s) 22
Bpu10I CCTNAGC 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 63, 69
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bse21I CCTNAGG 1 cut(s) 57
BseDI CCNNGG 2 cut(s) 63, 69
BseMII CTCAG 1 cut(s) 71
BseXI GCAGC 1 cut(s) 7
Bsh1236I CGCG 1 cut(s) 48
BshFI GGCC 2 cut(s) 62, 68
BsnI GGCC 2 cut(s) 62, 68
Bsp19I CCATGG 1 cut(s) 69
Bsp68I TCGCGA 1 cut(s) 48
BspANI GGCC 2 cut(s) 62, 68
BspCNI CTCAG 1 cut(s) 70
BspFNI CGCG 1 cut(s) 48
BssECI CCNNGG 2 cut(s) 63, 69
BssT1I CCWWGG 2 cut(s) 63, 69
BstC8I GCNNGC 1 cut(s) 46
BstDEI CTNAG 2 cut(s) 57, 135
BstDSI CCRYGG 1 cut(s) 69
BstFNI CGCG 1 cut(s) 48
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 1 cut(s) 7
BstXI CCANNNNNNTGG 1 cut(s) 70
Bsu36I CCTNAGG 1 cut(s) 57
BsuRI GGCC 2 cut(s) 62, 68
BtgI CCRYGG 1 cut(s) 69
BtgZI GCGATG 1 cut(s) 63
BtuMI TCGCGA 1 cut(s) 48
Cac8I GCNNGC 1 cut(s) 46
CviAII CATG 1 cut(s) 70
CviJI RGCY 5 cut(s) 20, 44, 62, 68, 128
CviKI_1 RGCY 5 cut(s) 20, 44, 62, 68, 128
DdeI CTNAG 2 cut(s) 57, 135
Eco130I CCWWGG 2 cut(s) 63, 69
Eco81I CCTNAGG 1 cut(s) 57
EcoT14I CCWWGG 2 cut(s) 63, 69
ErhI CCWWGG 2 cut(s) 63, 69
FaeI CATG 1 cut(s) 73
FaiI YATR 5 cut(s) 32, 71, 90, 107, 149
FatI CATG 1 cut(s) 69
Fnu4HI GCNGC 1 cut(s) 21
Fsp4HI GCNGC 1 cut(s) 21
GluI GCNGC 1 cut(s) 21
HaeIII GGCC 2 cut(s) 62, 68
Hin1II CATG 1 cut(s) 73
HinfI GANTC 1 cut(s) 121
Hpy188III TCNNGA 2 cut(s) 47, 101
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 1 cut(s) 92
HpyF3I CTNAG 2 cut(s) 57, 135
HpySE526I ACGT 1 cut(s) 78
Hsp92II CATG 1 cut(s) 73
LmnI GCTCC 1 cut(s) 17
LpnPI CCDG 1 cut(s) 44
Lsp1109I GCAGC 1 cut(s) 7
MaeII ACGT 1 cut(s) 78
MluCI AATT 2 cut(s) 150, 163
MnlI CCTC 2 cut(s) 3, 66
MvnI CGCG 1 cut(s) 48
NcoI CCATGG 1 cut(s) 69
NlaIII CATG 1 cut(s) 73
NruI TCGCGA 1 cut(s) 48
PfeI GAWTC 1 cut(s) 121
PkrI GCNGC 1 cut(s) 22
RruI TCGCGA 1 cut(s) 48
SatI GCNGC 1 cut(s) 21
SetI ASST 6 cut(s) 8, 22, 46, 81, 130, 141
Sse9I AATT 2 cut(s) 150, 163
StyI CCWWGG 2 cut(s) 63, 69
TaiI ACGT 1 cut(s) 81
TaqII GACCGA 1 cut(s) 125
TasI AATT 2 cut(s) 150, 163
TfiI GAWTC 1 cut(s) 121
TseI GCWGC 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.