RchiOBHm_Chr1g0382521

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002123
Physical Location & Seq
Forward (+)
67352596 .. 67358211
5616 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60549

Sequence Viewer

Length: 2529 bp
ATGGAGGCTACGGCCGCCGCGCGCGGTGGTTCGATGCCGGCGATGATGCAAGCTCAGACGCGCAAGCAGTGGCGCGCCGTCTCGGAGCATCATTCGGCTCGGAACATCGGCGATGAGGAGCTGGAGCGATCAAAGTTAGGCCATTCTGATGAGAGAACAGTATATGAGCAGGGAAGAGAGCCAGTTGATGTAGACTTTTGTTCGATCTCCATAGATGGGACTTTGGACCATGACCTCTTGCAGCAGCGGCTTCACGATATTGTCAGGCAAAGAGAGGAACTGCAGCATATGGAGACTGAGCTTAGAGCTCAAATGATTGCAAGATCTGAGATAATGGATATACAAAATAACTTTGATGCCCAACTGAAGGAGCATGCTAATGCTGCTTCCAAGCTTCAGGAGCAACTCCATGAAAAGGAGCAGGCCATACATGATTTGGAGAGGGAATTGGAAGAAAAAGATAGAGAGCTGCATGCTACCAAGTTAGATAATGAAGCGGCTTGGGCCAAAGAGGGCCTTCTTAGAGAACAGAACAAAGAATTAGCAACTTTCAGAAGAGAGCGTGACCATTCAGAAGCTGAAAGAGCCCAGCATATACAGCAATTACATGATCTTCAAGAACATATTCAAGAAAAAGAGAGGCAACTTATTGAGTTGCAGGAACAGCATAGGCTTGCTCAAGAAGCCATTCTATTTAAGGATGAACAACTGAGCGAAGCCCAAGCTTGGATCACCCGTGTTCAAGAAATGGATGCTTTGCAGTCAACTACACTTCAAAATCAGTTGCGGGAACACTCAGAACACTATAATCAGCTCTGGCTTGGTTGGCAAAGACAGTTTGCTGAAATGGAGAGACATCATATGCATACGGTACAACAGCTTCAGCTTGAGTTAGCTGATGCCAGACAAAGAAGTGGAACGTACACTGATGAATCACGTGCAGCAAATTCAACTTCAAAGGATGCATCTCAATTTGGTCGGAACAATGGAAACCAGATTGATATAAATACGTCAAATGGAAATACAGGGGCCCTCCCAAATGGGAATTCACATGACGTTTCATCGTTCTCTTCAACTGTCAATGCATCAAGTCAGGTTGACCATGTTCCTAGTGTTCCAATTGGTCCTTCTTCTCTACTTGGGATGCCTCCCTTTCTTCCACCTGGACAGGTGACTGGTATGCATCCATTTGTTCTGCATCAACCTGGGGTTCCCCATTCCATGCCACCACAGGTTCCCCAATCACATGTGGGGAATTTTCACTCAATACCTGCAATGTCATCTCTCCAACAGTGGCAAAACCAACAGGCTCCGTCCGAGAGTTTGCAGATAGCTACACAAACTGAACCTCCATCTTCCCAAAATGATCAAAACCTGATAAGATCAGATGCGAAGTATCACTATGAAACTTCTGTTAATGGGCAACAATTTCATCAAGACTATTTGGATGTTCAAATTCGCGAAGGGGCAGAGCCTGAGCCTGTGATATCATCTTCCCCCGAGGAAGCACAGGTTCTTGAGTCAATTAATAGCAGTTACTTGGTCTCACCCCAAACTGATCAGAGCTTGCAACAAATGTCTTCCCAATTTACTGATTCTTTAAGATTGGATTCCCTTGAGAAGAGCAGTGAAATCAAGGCACATGAGCAGAATGTTCAGACCTTGATCGATCATGGGTTGGACGGACAAGTTTTAACGGCTGAGAAACCAAATTCTGCTACCAAGTCCTCAAAATCTGACACTCCAATCCATTCAGTCAATCTCAATGAAATTACAATAAACAATTCCCCTGGTACAGGTTTGCCTGAGTCCTTTGTCTCAACTGGACACACCAATGCACCATCAGTGGGTAGGACTTCGGAGACCGCTCTTCTAGATGAAAGGTCATTGTTGGCTTGTATGGTTCGGACAATACCAGCTGGTGGTAGAATTAGGATCAGTTCGACGCTGCCAAATAGGCTCGGGAAGATGCTTGCACCTTTACACTGGCATGATTACAAGAAAAAGTATGGAAAGCTAGATGATTTCGTTGCTGCTCATACTGAACTATTTGTGATTGAGGGGGACTATATTCAGCTGCGTGAAGGAGCTCAAGAGATGATAGCAGCTACAGCCGCTGTTGCCAGAGTTGCTGCTGCTGCAGCAGCATCATCCCCCTACTCTTCATGTTTGCCTTCTGTGGCTGTTACACCAGTGGCACAGACTCACCGCTTAAAGAAGATATCATCTCTTGATTCCAAAAATGTGGTTACTTCTACTCCAAATGCAAATAATGCTCACTTGCAGTCTGTGAAGCAGAATCAGCAATTAAATGGTGTCTCTTTTGGTGTTTCGGGAGGTATGTCGAACGTAAAAATTTTGAGCAAATCCAAGGACATGAATGGGCCTGAACCTACTCCCAGCCAGTCATCTGTACTTCTTAATGGTGGAAATGGAGCTCCTCTGGACAGATCAATGGCCAATGGCCGGCATTTTGTTGGGAAACAGCATGGCAGGATGACTAATTCTGCATTTACCTCCAGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

842

Amino Acids

93.5

Weight (kDa)

5.72

Isoelectric Point (pI)

56.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7725 PF24851 624 - 694 8.6e-39 Domain of unknown function (DUF7725)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1279
AccB7I CCANNNNNTGG 1 cut(s) 1922
AccBSI CCGCTC 1 cut(s) 1868
AccI GTMKAC 1 cut(s) 192
AccII CGCG 6 cut(s) 20, 22, 24, 61, 75, 1461
AciI CCGC 9 cut(s) 15, 18, 24, 247, 497, 787, 1866, 2115, 2209
AclWI GGATC 2 cut(s) 737, 1943
AcoI YGGCCR 3 cut(s) 12, 2457, 2464
AcsI RAATTY 6 cut(s) 946, 1045, 1255, 1455, 1711, 2355
AcuI CTGAAG 3 cut(s) 380, 386, 866
AcvI CACGTG 1 cut(s) 938
AfaI GTAC 4 cut(s) 873, 923, 1795, 2415
AfiI CCNNNNNNNGG 8 cut(s) 216, 367, 415, 726, 1796, 1847, 1922, 2466
AflIII ACRYGT 1 cut(s) 1246
AgsI TTSAA 8 cut(s) 617, 629, 743, 776, 951, 957, 1074, 1454
AjnI CCWGG 3 cut(s) 1162, 1204, 1789
AjuI GAANNNNNNNTTGG 4 cut(s) 1030, 1062, 1111, 1143
Alw21I GWGCWC 3 cut(s) 310, 2092, 2440
Alw26I GTCTC 7 cut(s) 85, 287, 847, 1549, 1822, 1856, 2323
AlwI GGATC 2 cut(s) 737, 1943
AlwNI CAGNNNCTG 3 cut(s) 578, 1475, 2117
Ama87I CYCGRG 2 cut(s) 1499, 1961
AoxI GGCC 9 cut(s) 12, 139, 423, 504, 514, 1029, 2384, 2457, 2464
ApaI GGGCCC 1 cut(s) 1033
ApoI RAATTY 6 cut(s) 946, 1045, 1255, 1455, 1711, 2355
AscI GGCGCGCC 1 cut(s) 73
AseI ATTAAT 1 cut(s) 1527
Asp700I GAANNNNTTC 2 cut(s) 624, 687
AspLEI GCGC 5 cut(s) 22, 24, 63, 75, 77
AspS9I GGNCC 7 cut(s) 226, 504, 514, 1029, 1030, 1124, 2384
AsuHPI GGTGA 4 cut(s) 724, 1183, 1539, 2198
AvaI CYCGRG 2 cut(s) 1499, 1961
AvaII GGWCC 2 cut(s) 226, 1124
BaeGI GKGCMC 1 cut(s) 1033
BalI TGGCCA 1 cut(s) 2459
BanII GRGCYC 5 cut(s) 310, 589, 1033, 2092, 2440
BarI GAAGNNNNNNTAC 2 cut(s) 864, 896
BbrPI CACGTG 1 cut(s) 938
BbsI GAAGAC 1 cut(s) 1572
Bbv12I GWGCWC 3 cut(s) 310, 2092, 2440
BccI CCATC 3 cut(s) 209, 1360, 1849
BceAI ACGGC 3 cut(s) 27, 62, 1713
BciT130I CCWGG 3 cut(s) 1164, 1206, 1791
BclI TGATCA 2 cut(s) 1366, 1558
BcoDI GTCTC 7 cut(s) 85, 287, 847, 1549, 1822, 1856, 2323
BfaI CTAG 3 cut(s) 1110, 1874, 2018
BfmI CTRYAG 3 cut(s) 281, 2109, 2139
BfuAI ACCTGC 1 cut(s) 1279
BglI GCCNNNNNGGC 1 cut(s) 1957
BglII AGATCT 1 cut(s) 323
Bme1390I CCNGG 3 cut(s) 1164, 1206, 1791
Bme18I GGWCC 2 cut(s) 226, 1124
BmeT110I CYCGRG 2 cut(s) 1499, 1961
BmgT120I GGNCC 7 cut(s) 226, 504, 514, 1029, 1030, 1124, 2384
BmiI GGNNCC 5 cut(s) 1030, 1031, 1212, 1236, 1311
BmrFI CCNGG 3 cut(s) 1164, 1206, 1791
BpiI GAAGAC 1 cut(s) 1572
BpmI CTGGAG 2 cut(s) 143, 2503
Bpu10I CCTNAGC 1 cut(s) 1476
BpuEI CTTGAG 5 cut(s) 663, 908, 1538, 1637, 2076
Bsa29I ATCGAT 1 cut(s) 1668
BsaAI YACGTR 1 cut(s) 938
BsaI GGTCTC 2 cut(s) 1549, 1856
BsaJI CCNNGG 4 cut(s) 1205, 1500, 1789, 2370
BsaXI ACNNNNNCTCC 4 cut(s) 1333, 1363, 2242, 2272
Bsc4I CCNNNNNNNGG 8 cut(s) 216, 367, 415, 726, 1796, 1847, 1922, 2466
Bse118I RCCGGY 2 cut(s) 37, 2466
Bse1I ACTGG 6 cut(s) 182, 1180, 1828, 1991, 2192, 2404
Bse3DI GCAATG 1 cut(s) 1281
BseBI CCWGG 3 cut(s) 1164, 1206, 1791
BseCI ATCGAT 1 cut(s) 1668
BseDI CCNNGG 4 cut(s) 1205, 1500, 1789, 2370
BseGI GGATG 8 cut(s) 706, 757, 967, 1149, 1183, 1453, 2150, 2502
BseLI CCNNNNNNNGG 8 cut(s) 216, 367, 415, 726, 1796, 1847, 1922, 2466
BseMI GCAATG 1 cut(s) 1281
BseMII CTCAG 8 cut(s) 68, 288, 318, 701, 810, 1467, 1692, 1797
BseNI ACTGG 6 cut(s) 182, 1180, 1828, 1991, 2192, 2404
BsePI GCGCGC 2 cut(s) 20, 73
BseRI GAGGAG 2 cut(s) 131, 2430
BseSI GKGCMC 1 cut(s) 1033
BseX3I CGGCCG 1 cut(s) 12
BseYI CCCAGC 2 cut(s) 588, 2399
BsgI GTGCAG 1 cut(s) 960
Bsh1236I CGCG 6 cut(s) 20, 22, 24, 61, 75, 1461
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 9 cut(s) 14, 141, 425, 506, 516, 1031, 2386, 2459, 2466
BshVI ATCGAT 1 cut(s) 1668
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 3 cut(s) 310, 2092, 2440
BsiHKCI CYCGRG 2 cut(s) 1499, 1961
BsiSI CCGG 2 cut(s) 38, 2467
BslFI GGGAC 2 cut(s) 232, 2078
BslI CCNNNNNNNGG 8 cut(s) 216, 367, 415, 726, 1796, 1847, 1922, 2466
BsmAI GTCTC 7 cut(s) 85, 287, 847, 1549, 1822, 1856, 2323
BsmBI CGTCTC 1 cut(s) 85
BsmFI GGGAC 2 cut(s) 232, 2078
BsnI GGCC 9 cut(s) 14, 141, 425, 506, 516, 1031, 2386, 2459, 2466
Bso31I GGTCTC 2 cut(s) 1549, 1856
BsoBI CYCGRG 2 cut(s) 1499, 1961
Bsp120I GGGCCC 1 cut(s) 1029
Bsp1286I GDGCHC 5 cut(s) 310, 589, 1033, 2092, 2440
Bsp68I TCGCGA 1 cut(s) 1461
BspACI CCGC 9 cut(s) 15, 18, 24, 247, 497, 787, 1866, 2115, 2209
BspANI GGCC 9 cut(s) 14, 141, 425, 506, 516, 1031, 2386, 2459, 2466
BspCNI CTCAG 8 cut(s) 67, 289, 319, 702, 809, 1468, 1693, 1798
BspDI ATCGAT 1 cut(s) 1668
BspFNI CGCG 6 cut(s) 20, 22, 24, 61, 75, 1461
BspLI GGNNCC 5 cut(s) 1030, 1031, 1212, 1236, 1311
BspMAI CTGCAG 2 cut(s) 285, 2143
BspMI ACCTGC 1 cut(s) 1279
BspPI GGATC 2 cut(s) 737, 1943
BspQI GCTCTTC 2 cut(s) 1616, 1875
BspTNI GGTCTC 2 cut(s) 1549, 1856
BsrBI CCGCTC 1 cut(s) 1868
BsrDI GCAATG 1 cut(s) 1281
BsrFI RCCGGY 2 cut(s) 37, 2466
BsrI ACTGG 6 cut(s) 182, 1180, 1828, 1991, 2192, 2404
BssAI RCCGGY 2 cut(s) 37, 2466
BssECI CCNNGG 4 cut(s) 1205, 1500, 1789, 2370
BssHII GCGCGC 2 cut(s) 20, 73
BssT1I CCWWGG 1 cut(s) 2370
Bst2UI CCWGG 3 cut(s) 1164, 1206, 1791
Bst4CI ACNGT 5 cut(s) 160, 837, 871, 1078, 1293
Bst6I CTCTTC 6 cut(s) 169, 550, 1075, 1616, 1875, 2167
BstAPI GCANNNNNTGC 1 cut(s) 2273
BstBAI YACGTR 1 cut(s) 938
BstENI CCTNNNNNAGG 1 cut(s) 1794
BstF5I GGATG 8 cut(s) 706, 757, 967, 1149, 1183, 1453, 2150, 2502
BstFNI CGCG 6 cut(s) 20, 22, 24, 61, 75, 1461
BstHHI GCGC 5 cut(s) 22, 24, 63, 75, 77
BstMAI GTCTC 7 cut(s) 85, 287, 847, 1549, 1822, 1856, 2323
BstMCI CGRYCG 1 cut(s) 15
BstNI CCWGG 3 cut(s) 1164, 1206, 1791
BstNSI RCATGY 3 cut(s) 377, 476, 1250
BstSCI CCNGG 3 cut(s) 1162, 1204, 1789
BstSFI CTRYAG 3 cut(s) 281, 2109, 2139
BstSLI GKGCMC 1 cut(s) 1033
BstUI CGCG 6 cut(s) 20, 22, 24, 61, 75, 1461
BstV2I GAAGAC 1 cut(s) 1572
BstX2I RGATCY 1 cut(s) 323
BstXI CCANNNNNNTGG 1 cut(s) 2245
BstYI RGATCY 1 cut(s) 323
BstZI CGGCCG 1 cut(s) 12
Bsu15I ATCGAT 1 cut(s) 1668
BsuRI GGCC 9 cut(s) 14, 141, 425, 506, 516, 1031, 2386, 2459, 2466
BsuTUI ATCGAT 1 cut(s) 1668
BtgZI GCGATG 2 cut(s) 56, 126
BtsCI GGATG 8 cut(s) 706, 757, 967, 1149, 1183, 1453, 2150, 2502
BtsI GCAGTG 2 cut(s) 74, 1633
BtsIMutI CAGTG 7 cut(s) 74, 924, 1298, 1633, 1851, 1984, 2199
BtuMI TCGCGA 1 cut(s) 1461
BveI ACCTGC 1 cut(s) 1279
CaiI CAGNNNCTG 3 cut(s) 578, 1475, 2117
CfoI GCGC 5 cut(s) 22, 24, 63, 75, 77
Cfr10I RCCGGY 2 cut(s) 37, 2466
Cfr13I GGNCC 7 cut(s) 226, 504, 514, 1029, 1030, 1124, 2384
ClaI ATCGAT 1 cut(s) 1668
CseI GACGC 2 cut(s) 67, 1954
Csp6I GTAC 4 cut(s) 872, 922, 1794, 2414
CviQI GTAC 4 cut(s) 872, 922, 1794, 2414
EaeI YGGCCR 3 cut(s) 12, 2457, 2464
EagI CGGCCG 1 cut(s) 12
Eam1104I CTCTTC 6 cut(s) 169, 550, 1075, 1616, 1875, 2167
EarI CTCTTC 6 cut(s) 169, 550, 1075, 1616, 1875, 2167
Ecl136II GAGCTC 3 cut(s) 308, 2090, 2438
EclXI CGGCCG 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 2370
Eco24I GRGCYC 5 cut(s) 310, 589, 1033, 2092, 2440
Eco31I GGTCTC 2 cut(s) 1549, 1856
Eco32I GATATC 2 cut(s) 1488, 2223
Eco47I GGWCC 2 cut(s) 226, 1124
Eco52I CGGCCG 1 cut(s) 12
Eco53kI GAGCTC 3 cut(s) 308, 2090, 2438
Eco57I CTGAAG 3 cut(s) 380, 386, 866
Eco72I CACGTG 1 cut(s) 938
Eco88I CYCGRG 2 cut(s) 1499, 1961
EcoICRI GAGCTC 3 cut(s) 308, 2090, 2438
EcoNI CCTNNNNNAGG 1 cut(s) 1794
EcoO109I RGGNCCY 3 cut(s) 514, 1029, 1030
EcoRI GAATTC 1 cut(s) 1045
EcoRII CCWGG 3 cut(s) 1162, 1204, 1789
EcoRV GATATC 2 cut(s) 1488, 2223
EcoT14I CCWWGG 1 cut(s) 2370
EcoT22I ATGCAT 4 cut(s) 867, 967, 1087, 1185
EcoT38I GRGCYC 5 cut(s) 310, 589, 1033, 2092, 2440
ErhI CCWWGG 1 cut(s) 2370
Esp3I CGTCTC 1 cut(s) 85
FalI AAGNNNNNCTT 2 cut(s) 501, 533
FaqI GGGAC 2 cut(s) 232, 2078
FauI CCCGC 1 cut(s) 780
FauNDI CATATG 2 cut(s) 288, 861
FbaI TGATCA 2 cut(s) 1366, 1558
FblI GTMKAC 1 cut(s) 192
FokI GGATG 8 cut(s) 713, 764, 974, 1156, 1170, 1460, 2137, 2509
FriOI GRGCYC 5 cut(s) 310, 589, 1033, 2092, 2440
FspBI CTAG 3 cut(s) 1110, 1874, 2018
GlaI GCGC 5 cut(s) 21, 23, 62, 74, 76
GsaI CCCAGC 2 cut(s) 592, 2403
GsuI CTGGAG 2 cut(s) 143, 2503
HaeIII GGCC 9 cut(s) 14, 141, 425, 506, 516, 1031, 2386, 2459, 2466
HapII CCGG 2 cut(s) 38, 2467
HgaI GACGC 2 cut(s) 67, 1954
HhaI GCGC 5 cut(s) 22, 24, 63, 75, 77
Hin6I GCGC 5 cut(s) 20, 22, 61, 73, 75
HinP1I GCGC 5 cut(s) 20, 22, 61, 73, 75
HincII GTYRAC 2 cut(s) 765, 1099
HindII GTYRAC 2 cut(s) 765, 1099
HindIII AAGCTT 2 cut(s) 392, 723
HinfI GANTC 8 cut(s) 932, 1520, 1595, 1610, 1808, 2203, 2234, 2299
HpaII CCGG 2 cut(s) 38, 2467
HphI GGTGA 4 cut(s) 724, 1183, 1539, 2198
Hpy166II GTNNAC 4 cut(s) 193, 765, 924, 1099
Hpy8I GTNNAC 4 cut(s) 193, 765, 924, 1099
Hpy99I CGWCG 1 cut(s) 1948
HpyAV CCTTC 6 cut(s) 361, 527, 1137, 1457, 2078, 2184
HpyCH4III ACNGT 5 cut(s) 160, 837, 871, 1078, 1293
HpyCH4IV ACGT 5 cut(s) 920, 937, 1010, 1056, 2349
HpySE526I ACGT 5 cut(s) 920, 937, 1010, 1056, 2349
HspAI GCGC 5 cut(s) 20, 22, 61, 73, 75
KroI GCCGGC 2 cut(s) 37, 2466
KroNI GCCGGC 2 cut(s) 39, 2468
Ksp22I TGATCA 2 cut(s) 1366, 1558
LguI GCTCTTC 2 cut(s) 1616, 1875
MaeI CTAG 3 cut(s) 1110, 1874, 2018
MaeII ACGT 5 cut(s) 920, 937, 1010, 1056, 2349
MaeIII GTNAC 5 cut(s) 563, 1171, 1535, 2185, 2248
MauBI CGCGCGCG 1 cut(s) 20
MbiI CCGCTC 1 cut(s) 1868
MfeI CAATTG 1 cut(s) 1119
MflI RGATCY 1 cut(s) 323
MhlI GDGCHC 5 cut(s) 310, 589, 1033, 2092, 2440
MlsI TGGCCA 1 cut(s) 2459
MluNI TGGCCA 1 cut(s) 2459
MlyI GAGTC 3 cut(s) 1529, 1817, 2197
MmeI TCCRAC 3 cut(s) 959, 1312, 1659
Mox20I TGGCCA 1 cut(s) 2459
Mph1103I ATGCAT 4 cut(s) 867, 967, 1087, 1185
MroNI GCCGGC 2 cut(s) 37, 2466
MroXI GAANNNNTTC 2 cut(s) 624, 687
MscI TGGCCA 1 cut(s) 2459
MseI TTAA 8 cut(s) 696, 1416, 1527, 1601, 1694, 2213, 2309, 2421
MslI CAYNNNNRTG 4 cut(s) 147, 378, 1833, 1989
Msp20I TGGCCA 1 cut(s) 2459
MspA1I CMGCKG 4 cut(s) 247, 1919, 2077, 2117
MspI CCGG 2 cut(s) 38, 2467
MspR9I CCNGG 3 cut(s) 1164, 1206, 1791
MunI CAATTG 1 cut(s) 1119
MvaI CCWGG 3 cut(s) 1164, 1206, 1791
MvnI CGCG 6 cut(s) 20, 22, 24, 61, 75, 1461
NaeI GCCGGC 2 cut(s) 39, 2468
NdeI CATATG 2 cut(s) 288, 861
NgoMIV GCCGGC 2 cut(s) 37, 2466
NlaIV GGNNCC 5 cut(s) 1030, 1031, 1212, 1236, 1311
NmuCI GTSAC 2 cut(s) 563, 1171
NruI TCGCGA 1 cut(s) 1461
NsiI ATGCAT 4 cut(s) 867, 967, 1087, 1185
NspI RCATGY 3 cut(s) 377, 476, 1250
PaeI GCATGC 2 cut(s) 377, 476
PalAI GGCGCGCC 1 cut(s) 73
PauI GCGCGC 2 cut(s) 20, 73
PciI ACATGT 1 cut(s) 1246
PciSI GCTCTTC 2 cut(s) 1616, 1875
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PdiI GCCGGC 2 cut(s) 39, 2468
PdmI GAANNNNTTC 2 cut(s) 624, 687
PfeI GAWTC 5 cut(s) 932, 1595, 1610, 2234, 2299
PflMI CCANNNNNTGG 1 cut(s) 1922
PleI GAGTC 3 cut(s) 1528, 1816, 2197
PmaCI CACGTG 1 cut(s) 938
PmlI CACGTG 1 cut(s) 938
PpsI GAGTC 3 cut(s) 1528, 1816, 2197
Ppu21I YACGTR 1 cut(s) 938
PscI ACATGT 1 cut(s) 1246
PshBI ATTAAT 1 cut(s) 1527
Psp124BI GAGCTC 3 cut(s) 310, 2092, 2440
Psp6I CCWGG 3 cut(s) 1162, 1204, 1789
PspCI CACGTG 1 cut(s) 938
PspFI CCCAGC 2 cut(s) 588, 2399
PspGI CCWGG 3 cut(s) 1162, 1204, 1789
PspN4I GGNNCC 5 cut(s) 1030, 1031, 1212, 1236, 1311
PspOMI GGGCCC 1 cut(s) 1029
PspPI GGNCC 7 cut(s) 226, 504, 514, 1029, 1030, 1124, 2384
PstI CTGCAG 2 cut(s) 285, 2143
PstNI CAGNNNCTG 3 cut(s) 578, 1475, 2117
PsuI RGATCY 1 cut(s) 323
PteI GCGCGC 2 cut(s) 20, 73
PvuII CAGCTG 2 cut(s) 1919, 2077
RruI TCGCGA 1 cut(s) 1461
RsaI GTAC 4 cut(s) 873, 923, 1795, 2415
RsaNI GTAC 4 cut(s) 872, 922, 1794, 2414
RseI CAYNNNNRTG 4 cut(s) 147, 378, 1833, 1989
SacI GAGCTC 3 cut(s) 310, 2092, 2440
SapI GCTCTTC 2 cut(s) 1616, 1875
SaqAI TTAA 8 cut(s) 696, 1416, 1527, 1601, 1694, 2213, 2309, 2421
Sau96I GGNCC 7 cut(s) 226, 504, 514, 1029, 1030, 1124, 2384
SchI GAGTC 3 cut(s) 1529, 1817, 2197
ScrFI CCNGG 3 cut(s) 1164, 1206, 1791
SduI GDGCHC 5 cut(s) 310, 589, 1033, 2092, 2440
SfcI CTRYAG 3 cut(s) 281, 2109, 2139
SgsI GGCGCGCC 1 cut(s) 73
SinI GGWCC 2 cut(s) 226, 1124
SmiMI CAYNNNNRTG 4 cut(s) 147, 378, 1833, 1989
SmlI CTYRAG 5 cut(s) 678, 887, 1517, 1616, 2091
SmoI CTYRAG 5 cut(s) 678, 887, 1517, 1616, 2091
SphI GCATGC 2 cut(s) 377, 476
SsiI CCGC 9 cut(s) 15, 18, 24, 247, 497, 787, 1866, 2115, 2209
SspMI CTAG 3 cut(s) 1110, 1874, 2018
SstI GAGCTC 3 cut(s) 310, 2092, 2440
StyD4I CCNGG 3 cut(s) 1162, 1204, 1789
StyI CCWWGG 1 cut(s) 2370
TaaI ACNGT 5 cut(s) 160, 837, 871, 1078, 1293
TaiI ACGT 5 cut(s) 923, 940, 1013, 1059, 2352
TaqI TCGA 5 cut(s) 32, 203, 1668, 1943, 2345
TatI WGTACW 1 cut(s) 2413
TauI GCSGC 5 cut(s) 17, 20, 250, 500, 2117
TfiI GAWTC 5 cut(s) 932, 1595, 1610, 2234, 2299
Tru1I TTAA 8 cut(s) 696, 1416, 1527, 1601, 1694, 2213, 2309, 2421
Tru9I TTAA 8 cut(s) 696, 1416, 1527, 1601, 1694, 2213, 2309, 2421
TscAI CASTG 7 cut(s) 74, 931, 1298, 1633, 1851, 1991, 2199
TseFI GTSAC 2 cut(s) 563, 1171
Tsp45I GTSAC 2 cut(s) 563, 1171
TspGWI ACGGA 2 cut(s) 1302, 1698
TspRI CASTG 7 cut(s) 74, 931, 1298, 1633, 1851, 1991, 2199
Van91I CCANNNNNTGG 1 cut(s) 1922
VpaK11BI GGWCC 2 cut(s) 226, 1124
VspI ATTAAT 1 cut(s) 1527
XagI CCTNNNNNAGG 1 cut(s) 1794
XapI RAATTY 6 cut(s) 946, 1045, 1255, 1455, 1711, 2355
XbaI TCTAGA 1 cut(s) 1873
XceI RCATGY 3 cut(s) 377, 476, 1250
XcmI CCANNNNNNNNNTGG 1 cut(s) 433
XmiI GTMKAC 1 cut(s) 192
XmnI GAANNNNTTC 2 cut(s) 624, 687
XspI CTAG 3 cut(s) 1110, 1874, 2018
Zsp2I ATGCAT 4 cut(s) 867, 967, 1087, 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.