Rorug01G0435100

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002123
Physical Location & Seq
Reverse (-)
53442087 .. 53453734
11648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0435100.1

Sequence Viewer

Length: 1407 bp
ATGAGAATGAGAGAGGTTCGAGCATCCTTGAATTGCACTTGCGAAGCAGAGAAAGACATGGAGAATAATGGAAGGAACGTATTGATGAATATGGAAATAAAGGAGATGGTGAGAGGGAAGATTGAGATGAAAAGAATCGAAAATGCGACAAGGAGGCAGGTAACCTTTTCGAAACGTCGAAACGGGCTGTTGAAGAAGGCTTATGAGCTTTCGGTTCTTTGCGATGCAGAAGTTGCAGTCATCGTCTTTTCTCAGAAAAACAGGCTCTACGAGTTTTCCAGCTCTAACATGCAAAAGACTATAAAACAATACCATAAACATGCAAAAGGTGCGCTAACCAACACTACTGAAATGGAACAGTATATGCAGCAATTGAAGCATGAATCAGCTGACATGACCAAGAAGATCGAGATCCTAGAAGCTTCTCAACGGAGGATTTTGGGACATGACTTGGATTCTTGCTCTGCTCAAGAACTCAATCAGATGAGTAGCCAGCTCGACCGAAGCTTGCACATTGTACGAGAGAGAAAGGCTCAGCTATTCATGGAGCAGATAGAACGAGTAAAAGCAAAGGAGAGGCTTCTCTTAGGAGAGAATGCAAAACTACACACAAGGTGTGGTGCAAGGCCTTGTCAGAAACCTTTAGTTCAAGAAAAAGGAGTTGGGGCGGCAAGTTATAACTGGATGAATAGTAGTCCAAGTAGCCGCAGCCAAACAATTAGTAATTCAGATCAGATGGTGAGAGGGAAGATTGAGGTGAAAAGAATCGAAAATGCGACAAGCAGGCAGGTAACCTTTTCGAAACGTCGAAACGGGCTGCTGAAGAAGGCTTTTGAGCTTTCGGTTCTTTGCGATGCCGAAGTTGCAGTCATCATCTTTTCTCCGAAGGGCAGGCTCTATGAGTTTTCTAGCTCTGACATACAAAAGACTATAAAAAAATACCATAAACATGCAAAAGGTGCGCTAACCAACACTACTGAAGTGGAACAGTATATGCAGCAATTGAAGGATGAATCAGCTGACATGGCCAAGAAGATCGAGACCCTAGAAGCTTCTCAACGGAGGCTTTTGGGACATGACTTGGATCCTTGCTCTTCTGAAGAAGTCAATCAGATGAGTAGCCAGCTCGAGCGAAGCTTGCGCATTGTACGAGAGAGAAAGGATCAGCTATTCACGGAGCAGATACTAAAAGCAAAGGAGAGGCTCCTCTTAGAAGAGAATGCAAAACTACACACAGAGTGTGGTGCAAGGCCTTGTCAGAAACCTTTAGTTCAAGAAAAAGGAGTTGGGGCAGCAAGTTATAACTGGATGGATAGTAGTCAAAGTAGCAGCAGCCAAACAATTAATAATTCAGATCAGGTAGAGACTAGATTGTTCATCGGACCGCCAGTGATGCGCTGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

468

Amino Acids

53.65

Weight (kDa)

9.31

Isoelectric Point (pI)

53.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 45 - 92 1.9e-26 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 120 - 205 1.2e-21 K-box region
SRF-TF PF00319 255 - 302 8.8e-27 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 331 - 413 6.5e-19 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 678, 1302
Acc16I TGCGCA 1 cut(s) 1142
Acc36I ACCTGC 2 cut(s) 148, 778
AciI CCGC 3 cut(s) 668, 706, 1385
AclWI GGATC 4 cut(s) 406, 1079, 1092, 1170
AcoI YGGCCR 1 cut(s) 1026
AcuI CTGAAG 3 cut(s) 842, 999, 1119
AdeI CACNNNGTG 2 cut(s) 615, 1239
AfaI GTAC 2 cut(s) 519, 1149
AgsI TTSAA 6 cut(s) 31, 193, 376, 650, 1006, 1274
AjuI GAANNNNNNNTTGG 4 cut(s) 645, 677, 1269, 1301
Alw26I GTCTC 2 cut(s) 1034, 1358
AlwI GGATC 4 cut(s) 406, 1079, 1092, 1170
Ama87I CYCGRG 1 cut(s) 1127
AoxI GGCC 3 cut(s) 626, 1026, 1250
ApeKI GCWGC 7 cut(s) 367, 708, 817, 997, 1292, 1329, 1332
ArsI GACNNNNNNTTYG 2 cut(s) 852, 884
AseI ATTAAT 1 cut(s) 1344
AspLEI GCGC 4 cut(s) 334, 964, 1143, 1398
AspS9I GGNCC 1 cut(s) 1382
AsuHPI GGTGA 3 cut(s) 121, 751, 769
AsuII TTCGAA 2 cut(s) 170, 800
AvaI CYCGRG 1 cut(s) 1127
AvaII GGWCC 1 cut(s) 1382
BalI TGGCCA 1 cut(s) 1028
BamHI GGATCC 1 cut(s) 1084
BbvI GCAGC 7 cut(s) 379, 720, 804, 1009, 1304, 1341, 1344
BccI CCATC 3 cut(s) 100, 730, 1303
BcoDI GTCTC 2 cut(s) 1034, 1358
BfaI CTAG 4 cut(s) 416, 909, 1046, 1368
BfuAI ACCTGC 2 cut(s) 148, 778
BisI GCNGC 9 cut(s) 368, 669, 706, 709, 818, 998, 1293, 1330, 1333
BlpI GCTNAGC 1 cut(s) 534
BlsI GCNGC 9 cut(s) 369, 670, 707, 710, 819, 999, 1294, 1331, 1334
Bme18I GGWCC 1 cut(s) 1382
BmeT110I CYCGRG 1 cut(s) 1127
BmgT120I GGNCC 1 cut(s) 1382
BmiI GGNNCC 2 cut(s) 1086, 1205
BmsI GCATC 4 cut(s) 32, 214, 844, 1383
BplI GAGNNNNNCTC 2 cut(s) 517, 549
Bpu1102I GCTNAGC 1 cut(s) 534
Bpu14I TTCGAA 2 cut(s) 170, 800
BpuEI CTTGAG 1 cut(s) 453
BsaBI GATNNNNATC 1 cut(s) 410
BsaI GGTCTC 1 cut(s) 1034
Bse1I ACTGG 3 cut(s) 686, 1310, 1388
Bse8I GATNNNNATC 1 cut(s) 410
BseGI GGATG 4 cut(s) 23, 690, 1015, 1314
BseJI GATNNNNATC 1 cut(s) 410
BseMII CTCAG 2 cut(s) 266, 548
BseNI ACTGG 3 cut(s) 686, 1310, 1388
BseRI GAGGAG 1 cut(s) 1196
BseXI GCAGC 7 cut(s) 379, 720, 804, 1009, 1304, 1341, 1344
Bsh1285I CGRYCG 1 cut(s) 502
BshFI GGCC 3 cut(s) 628, 1028, 1252
BsiEI CGRYCG 1 cut(s) 502
BsiHKCI CYCGRG 1 cut(s) 1127
BslFI GGGAC 2 cut(s) 456, 1086
BsmAI GTCTC 2 cut(s) 1034, 1358
BsmFI GGGAC 2 cut(s) 456, 1086
BsmI GAATGC 2 cut(s) 601, 1225
BsnI GGCC 3 cut(s) 628, 1028, 1252
Bso31I GGTCTC 1 cut(s) 1034
BsoBI CYCGRG 1 cut(s) 1127
Bsp119I TTCGAA 2 cut(s) 170, 800
Bsp143I GATC 7 cut(s) 405, 411, 730, 1035, 1084, 1162, 1354
Bsp1720I GCTNAGC 1 cut(s) 534
BspACI CCGC 3 cut(s) 668, 706, 1385
BspANI GGCC 3 cut(s) 628, 1028, 1252
BspCNI CTCAG 2 cut(s) 265, 547
BspLI GGNNCC 2 cut(s) 1086, 1205
BspMI ACCTGC 2 cut(s) 148, 778
BspPI GGATC 4 cut(s) 406, 1079, 1092, 1170
BspQI GCTCTTC 1 cut(s) 1099
BspT104I TTCGAA 2 cut(s) 170, 800
BspTNI GGTCTC 1 cut(s) 1034
BsrI ACTGG 3 cut(s) 686, 1310, 1388
BssMI GATC 7 cut(s) 405, 411, 730, 1035, 1084, 1162, 1354
Bst4CI ACNGT 2 cut(s) 360, 990
Bst6I CTCTTC 2 cut(s) 1099, 1209
BstAPI GCANNNNNTGC 3 cut(s) 233, 329, 959
BstBI TTCGAA 2 cut(s) 170, 800
BstC8I GCNNGC 6 cut(s) 494, 509, 785, 893, 1124, 1139
BstDEI CTNAG 4 cut(s) 252, 534, 586, 1210
BstEII GGTNACC 2 cut(s) 160, 790
BstF5I GGATG 4 cut(s) 23, 690, 1015, 1314
BstHHI GCGC 4 cut(s) 334, 964, 1143, 1398
BstKTI GATC 7 cut(s) 408, 414, 733, 1038, 1087, 1165, 1357
BstMAI GTCTC 2 cut(s) 1034, 1358
BstMBI GATC 7 cut(s) 405, 411, 730, 1035, 1084, 1162, 1354
BstMCI CGRYCG 1 cut(s) 502
BstMWI GCNNNNNNNGC 8 cut(s) 233, 329, 376, 863, 959, 1025, 1138, 1393
BstNSI RCATGY 3 cut(s) 292, 323, 953
BstPI GGTNACC 2 cut(s) 160, 790
BstV1I GCAGC 7 cut(s) 379, 720, 804, 1009, 1304, 1341, 1344
BstX2I RGATCY 2 cut(s) 411, 1084
BstYI RGATCY 2 cut(s) 411, 1084
BsuRI GGCC 3 cut(s) 628, 1028, 1252
BtgZI GCGATG 2 cut(s) 237, 867
BtsCI GGATG 4 cut(s) 23, 690, 1015, 1314
BtsIMutI CAGTG 1 cut(s) 1395
BveI ACCTGC 2 cut(s) 148, 778
Cac8I GCNNGC 6 cut(s) 494, 509, 785, 893, 1124, 1139
CfoI GCGC 4 cut(s) 334, 964, 1143, 1398
Cfr13I GGNCC 1 cut(s) 1382
CpoI CGGWCCG 1 cut(s) 1382
Csp6I GTAC 2 cut(s) 518, 1148
CspI CGGWCCG 1 cut(s) 1382
CviQI GTAC 2 cut(s) 518, 1148
DdeI CTNAG 4 cut(s) 252, 534, 586, 1210
DpnI GATC 7 cut(s) 407, 413, 732, 1037, 1086, 1164, 1356
DpnII GATC 7 cut(s) 405, 411, 730, 1035, 1084, 1162, 1354
DraIII CACNNNGTG 2 cut(s) 615, 1239
EaeI YGGCCR 1 cut(s) 1026
Eam1104I CTCTTC 2 cut(s) 1099, 1209
EarI CTCTTC 2 cut(s) 1099, 1209
Eco147I AGGCCT 2 cut(s) 628, 1252
Eco31I GGTCTC 1 cut(s) 1034
Eco47I GGWCC 1 cut(s) 1382
Eco57I CTGAAG 3 cut(s) 842, 999, 1119
Eco88I CYCGRG 1 cut(s) 1127
Eco91I GGTNACC 2 cut(s) 160, 790
EcoO65I GGTNACC 2 cut(s) 160, 790
FaqI GGGAC 2 cut(s) 456, 1086
Fnu4HI GCNGC 9 cut(s) 368, 669, 706, 709, 818, 998, 1293, 1330, 1333
FokI GGATG 4 cut(s) 10, 697, 1022, 1321
Fsp4HI GCNGC 9 cut(s) 368, 669, 706, 709, 818, 998, 1293, 1330, 1333
FspBI CTAG 4 cut(s) 416, 909, 1046, 1368
FspI TGCGCA 1 cut(s) 1142
GlaI GCGC 4 cut(s) 333, 963, 1142, 1397
GluI GCNGC 9 cut(s) 368, 669, 706, 709, 818, 998, 1293, 1330, 1333
HaeIII GGCC 3 cut(s) 628, 1028, 1252
HhaI GCGC 4 cut(s) 334, 964, 1143, 1398
Hin6I GCGC 4 cut(s) 332, 962, 1141, 1396
HinP1I GCGC 4 cut(s) 332, 962, 1141, 1396
HindIII AAGCTT 4 cut(s) 420, 505, 1050, 1135
HinfI GANTC 5 cut(s) 135, 383, 455, 765, 1013
HphI GGTGA 3 cut(s) 121, 751, 769
Hpy188III TCNNGA 5 cut(s) 409, 470, 650, 1039, 1274
Hpy99I CGWCG 2 cut(s) 180, 810
HpyAV CCTTC 5 cut(s) 66, 190, 820, 880, 1000
HpyCH4III ACNGT 2 cut(s) 360, 990
HpyCH4IV ACGT 3 cut(s) 78, 175, 805
HpyF10VI GCNNNNNNNGC 8 cut(s) 233, 329, 376, 863, 959, 1025, 1138, 1393
HpyF3I CTNAG 4 cut(s) 252, 534, 586, 1210
HpySE526I ACGT 3 cut(s) 78, 175, 805
HspAI GCGC 4 cut(s) 332, 962, 1141, 1396
Kzo9I GATC 7 cut(s) 405, 411, 730, 1035, 1084, 1162, 1354
LguI GCTCTTC 1 cut(s) 1099
LmnI GCTCC 3 cut(s) 547, 1177, 1209
Lsp1109I GCAGC 7 cut(s) 379, 720, 804, 1009, 1304, 1341, 1344
LweI GCATC 4 cut(s) 32, 214, 844, 1383
MaeI CTAG 4 cut(s) 416, 909, 1046, 1368
MaeII ACGT 3 cut(s) 78, 175, 805
MaeIII GTNAC 2 cut(s) 160, 790
MalI GATC 7 cut(s) 407, 413, 732, 1037, 1086, 1164, 1356
MboI GATC 7 cut(s) 405, 411, 730, 1035, 1084, 1162, 1354
MboII GAAGA 9 cut(s) 130, 205, 415, 760, 835, 1045, 1086, 1112, 1226
MfeI CAATTG 2 cut(s) 371, 1001
MflI RGATCY 2 cut(s) 411, 1084
MlsI TGGCCA 1 cut(s) 1028
MluCI AATT 7 cut(s) 31, 371, 717, 724, 1001, 1341, 1348
MluNI TGGCCA 1 cut(s) 1028
Mox20I TGGCCA 1 cut(s) 1028
MscI TGGCCA 1 cut(s) 1028
MseI TTAA 1 cut(s) 1344
MslI CAYNNNNRTG 2 cut(s) 318, 948
Msp20I TGGCCA 1 cut(s) 1028
MspA1I CMGCKG 2 cut(s) 389, 1019
MunI CAATTG 2 cut(s) 371, 1001
Mva1269I GAATGC 2 cut(s) 601, 1225
MwoI GCNNNNNNNGC 8 cut(s) 233, 329, 376, 863, 959, 1025, 1138, 1393
NdeII GATC 7 cut(s) 405, 411, 730, 1035, 1084, 1162, 1354
NlaIV GGNNCC 2 cut(s) 1086, 1205
NsbI TGCGCA 1 cut(s) 1142
NspI RCATGY 3 cut(s) 292, 323, 953
NspV TTCGAA 2 cut(s) 170, 800
PaeR7I CTCGAG 1 cut(s) 1127
PceI AGGCCT 2 cut(s) 628, 1252
PciSI GCTCTTC 1 cut(s) 1099
PctI GAATGC 2 cut(s) 601, 1225
PfeI GAWTC 5 cut(s) 135, 383, 455, 765, 1013
PkrI GCNGC 9 cut(s) 369, 670, 707, 710, 819, 999, 1294, 1331, 1334
PshBI ATTAAT 1 cut(s) 1344
PsiI TTATAA 2 cut(s) 678, 1302
PspEI GGTNACC 2 cut(s) 160, 790
PspN4I GGNNCC 2 cut(s) 1086, 1205
PspPI GGNCC 1 cut(s) 1382
PspXI VCTCGAGB 1 cut(s) 1127
PsuI RGATCY 2 cut(s) 411, 1084
PvuII CAGCTG 2 cut(s) 389, 1019
RsaI GTAC 2 cut(s) 519, 1149
RsaNI GTAC 2 cut(s) 518, 1148
RseI CAYNNNNRTG 2 cut(s) 318, 948
Rsr2I CGGWCCG 1 cut(s) 1382
RsrII CGGWCCG 1 cut(s) 1382
SapI GCTCTTC 1 cut(s) 1099
SaqAI TTAA 1 cut(s) 1344
SatI GCNGC 9 cut(s) 368, 669, 706, 709, 818, 998, 1293, 1330, 1333
Sau3AI GATC 7 cut(s) 405, 411, 730, 1035, 1084, 1162, 1354
Sau96I GGNCC 1 cut(s) 1382
SfaNI GCATC 4 cut(s) 32, 214, 844, 1383
Sfr274I CTCGAG 1 cut(s) 1127
SfuI TTCGAA 2 cut(s) 170, 800
SinI GGWCC 1 cut(s) 1382
SlaI CTCGAG 1 cut(s) 1127
SmiMI CAYNNNNRTG 2 cut(s) 318, 948
SmlI CTYRAG 2 cut(s) 468, 1127
SmoI CTYRAG 2 cut(s) 468, 1127
Sse9I AATT 7 cut(s) 31, 371, 717, 724, 1001, 1341, 1348
SseBI AGGCCT 2 cut(s) 628, 1252
SsiI CCGC 3 cut(s) 668, 706, 1385
SspMI CTAG 4 cut(s) 416, 909, 1046, 1368
StuI AGGCCT 2 cut(s) 628, 1252
TaaI ACNGT 2 cut(s) 360, 990
TaiI ACGT 3 cut(s) 81, 178, 808
TaqII GACCGA 1 cut(s) 516
TasI AATT 7 cut(s) 31, 371, 717, 724, 1001, 1341, 1348
TauI GCSGC 2 cut(s) 671, 708
TfiI GAWTC 5 cut(s) 135, 383, 455, 765, 1013
Tru1I TTAA 1 cut(s) 1344
Tru9I TTAA 1 cut(s) 1344
TscAI CASTG 1 cut(s) 1395
TseI GCWGC 7 cut(s) 367, 708, 817, 997, 1292, 1329, 1332
TspDTI ATGAA 7 cut(s) 101, 143, 396, 532, 701, 1026, 1366
TspGWI ACGGA 3 cut(s) 445, 1075, 1190
TspRI CASTG 1 cut(s) 1395
VpaK11BI GGWCC 1 cut(s) 1382
VspI ATTAAT 1 cut(s) 1344
XceI RCATGY 3 cut(s) 292, 323, 953
XhoI CTCGAG 1 cut(s) 1127
XspI CTAG 4 cut(s) 416, 909, 1046, 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.