Rh1BG416500

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0002123
Physical Location & Seq
Forward (+)
53857551 .. 53863018
5468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG416500.1

Sequence Viewer

Length: 1632 bp
ATGAGTTTAATCAAGAAACCGGGACGGGATGGAGTTGCGGTGAGTCGAGGAGATGGTCGCGATGGGGTAGTCTCTGCTGGTCAAGAACAATGTGTTCAGCCTGCTCCTTTTGGACCCTTACCAAAGGGATATGTATGGGATCGCCTATCTGTCTCTACCAGCGGTGTTGGACGTCCTCTCTGTGCAGTTCCTATGTGTTGTGTTATTTGCAACAAGTTTCTTGATCACATGACTGAAGACTGCCCTCATTTGCATGATGAGAGTTACAGCGATCGCCGTTGGAAGGTTGGCCATGTTCCTAGTGTTCCAATTGGTCCTTTGTCTCTACTTGGGATGCCTCCCTTTCTTCCACCCGGACAGGCTCTGTCCGAGAGTTTGCAGATAGCTACACAAACTGAACCTCCATCTTCCCAAAATGATCAAAACCTGATAAGATCAGATGCAAAGTATCACTGTGTAACTTCTGTTAATGGGCAACCATTTCATCAAGACTATTTGGATGTTCAAATTCGCGAAGGGGCAGAGCCTGAGCCTGTGATATCATCTTCCCCCGAGGAAGCATATGTTCTTGAGTCAATTAATAGCAGTTACTTGGTCTCACCCCAAACTGATCAGAGCTTGCAACAAATGTCTTCCCAATTTACTGATTCTTTAAGATTGGATTCCCTTGAGAAGAGCAATGAAACCAAGGCACATGAGCAGAATGTTCAGACCTTGATCGATCATGGGTTGGACGGACAATTTTTAACGGCTGAGAAACCAAATTCTGCTACCAAGTCCTCAAAATCTGACACTCCAATCCATTCAGTCAATCTCAATGAAATTACAATAAACAATTCCCCTGGTACAGGTTTGCCTGAGTCCTTTGTCTCAACTGGACACACCAATGCACCATCAGTGGGTAGGACTTCGGAGACCGCTCTTCTAGATGAAAGGTCATTGTTGGCTTGCATGGTTCGGACAATACCAGCTGGTGGTAGAATTAGGATCAGTTCGACGCTGCCAAATAGGCTCGGGAAGATGCTTGCACCTTTACACTGGCATGATTACAAGAAAAAGTATGGAAAGCTAGATGATTTCGTTGCTGCTCATACTGAAGAGAGTTCTGCAGATGATTTCAAATGCATCTATGTTTGTCTATTTGTGATTGAGGGGGACTATATTCAGCTGCGTGAAGGAGCTCAAGAGATGATAGCAGCTACAGCCGCCGTTGCCAGAGTTGCTGCTGCTGCAGCAGCATCATCCCCCTACTCTTCATGTTTGCCTTCTGTGGCTGTTACTCCAGTGGCACAGACTCACCGCTTAAAGAAGATATCATCTCTTGATTCCAAAAATGTGGTTACTTCTACTCCAAATGCAAATAATGCTCACTTGCAGTCTGTGAAGCAGAATCAGCAATTAAATGGTGTATCTTTTGGTGTTTCTGGAGGAATGTCGAATGTAACAATTTTGAGCAAATCCAAGGACATGAATGGGCCTGAACCTACTCCCAGTCTGTCATCTGTACTTCTTAATGGTGGAAATGGAGCTCCTCTTGACAGATCAATGGCCAATGGCCGGCATTTTGTTGGGAAACAGCATGGCAGGTATGCCTCTAAACCTTCAAACATACTTGGAAGACACTTAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

543

Amino Acids

58.35

Weight (kDa)

6.41

Isoelectric Point (pI)

52.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7725 PF24851 308 - 369 4.9e-34 Domain of unknown function (DUF7725)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 175
Acc36I ACCTGC 1 cut(s) 1575
AccB7I CCANNNNNTGG 1 cut(s) 974
AccBSI CCGCTC 1 cut(s) 920
AccII CGCG 2 cut(s) 60, 513
AciI CCGC 5 cut(s) 38, 162, 918, 1206, 1300
AclWI GGATC 2 cut(s) 147, 995
AcoI YGGCCR 3 cut(s) 289, 1548, 1555
AcsI RAATTY 2 cut(s) 507, 763
AcuI CTGAAG 2 cut(s) 255, 1116
AcyI GRCGYC 1 cut(s) 172
AfaI GTAC 2 cut(s) 847, 1506
AfiI CCNNNNNNNGG 5 cut(s) 283, 848, 899, 974, 1557
AgsI TTSAA 3 cut(s) 506, 1120, 1605
AjnI CCWGG 1 cut(s) 841
AluBI AGCT 8 cut(s) 386, 618, 971, 1069, 1168, 1181, 1199, 1529
AluI AGCT 8 cut(s) 386, 618, 971, 1069, 1168, 1181, 1199, 1529
Alw21I GWGCWC 2 cut(s) 1183, 1531
Alw26I GTCTC 6 cut(s) 76, 157, 327, 601, 874, 908
AlwI GGATC 2 cut(s) 147, 995
AlwNI CAGNNNCTG 2 cut(s) 364, 527
Ama87I CYCGRG 2 cut(s) 551, 1013
AoxI GGCC 4 cut(s) 289, 1475, 1548, 1555
ApeKI GCWGC 9 cut(s) 1000, 1085, 1168, 1196, 1223, 1226, 1229, 1232, 1235
ApoI RAATTY 2 cut(s) 507, 763
AseI ATTAAT 1 cut(s) 579
AsiSI GCGATCGC 1 cut(s) 274
AspS9I GGNCC 3 cut(s) 113, 314, 1475
AsuC2I CCSGG 2 cut(s) 21, 354
AsuHPI GGTGA 3 cut(s) 52, 591, 1289
AvaI CYCGRG 2 cut(s) 551, 1013
AvaII GGWCC 2 cut(s) 113, 314
BalI TGGCCA 2 cut(s) 291, 1550
BanII GRGCYC 2 cut(s) 1183, 1531
BbsI GAAGAC 3 cut(s) 243, 624, 1624
Bbv12I GWGCWC 2 cut(s) 1183, 1531
BbvI GCAGC 9 cut(s) 987, 1072, 1155, 1208, 1210, 1213, 1216, 1244, 1247
BccI CCATC 5 cut(s) 23, 47, 56, 412, 901
BceAI ACGGC 3 cut(s) 261, 765, 1193
BciT130I CCWGG 1 cut(s) 843
BclI TGATCA 3 cut(s) 223, 418, 610
BcnI CCSGG 2 cut(s) 21, 354
BcoDI GTCTC 6 cut(s) 76, 157, 327, 601, 874, 908
BfaI CTAG 4 cut(s) 300, 926, 1070, 1630
BfmI CTRYAG 3 cut(s) 1107, 1200, 1230
BfuAI ACCTGC 1 cut(s) 1575
BglI GCCNNNNNGGC 1 cut(s) 1009
Bme1390I CCNGG 3 cut(s) 21, 354, 843
Bme18I GGWCC 2 cut(s) 113, 314
BmeT110I CYCGRG 2 cut(s) 551, 1013
BmgT120I GGNCC 3 cut(s) 113, 314, 1475
BmiI GGNNCC 1 cut(s) 115
BmrFI CCNGG 3 cut(s) 21, 354, 843
BmrI ACTGGG 1 cut(s) 1485
BmsI GCATC 5 cut(s) 324, 430, 1011, 1134, 1247
BmuI ACTGGG 1 cut(s) 1485
BpiI GAAGAC 3 cut(s) 243, 624, 1624
BpmI CTGGAG 2 cut(s) 1266, 1446
Bpu10I CCTNAGC 1 cut(s) 528
BpuEI CTTGAG 3 cut(s) 590, 689, 1167
BpuMI CCSGG 2 cut(s) 21, 354
Bsa29I ATCGAT 1 cut(s) 720
BsaHI GRCGYC 1 cut(s) 172
BsaI GGTCTC 2 cut(s) 601, 908
BsaJI CCNNGG 4 cut(s) 552, 687, 841, 1461
BsaXI ACNNNNNCTCC 6 cut(s) 24, 54, 385, 415, 1333, 1363
Bsc4I CCNNNNNNNGG 5 cut(s) 283, 848, 899, 974, 1557
Bse118I RCCGGY 1 cut(s) 1557
Bse1I ACTGG 4 cut(s) 880, 1043, 1283, 1491
Bse3DI GCAATG 1 cut(s) 685
BseBI CCWGG 1 cut(s) 843
BseCI ATCGAT 1 cut(s) 720
BseDI CCNNGG 4 cut(s) 552, 687, 841, 1461
BseGI GGATG 4 cut(s) 34, 339, 505, 1241
BseLI CCNNNNNNNGG 5 cut(s) 283, 848, 899, 974, 1557
BseMI GCAATG 1 cut(s) 685
BseMII CTCAG 3 cut(s) 519, 744, 849
BseNI ACTGG 4 cut(s) 880, 1043, 1283, 1491
BseRI GAGGAG 2 cut(s) 63, 1521
BseXI GCAGC 9 cut(s) 987, 1072, 1155, 1208, 1210, 1213, 1216, 1244, 1247
BsgI GTGCAG 1 cut(s) 204
Bsh1236I CGCG 2 cut(s) 60, 513
Bsh1285I CGRYCG 1 cut(s) 274
BshFI GGCC 4 cut(s) 291, 1477, 1550, 1557
BshVI ATCGAT 1 cut(s) 720
BsiEI CGRYCG 1 cut(s) 274
BsiHKAI GWGCWC 2 cut(s) 1183, 1531
BsiHKCI CYCGRG 2 cut(s) 551, 1013
BsiSI CCGG 3 cut(s) 20, 354, 1558
BslFI GGGAC 2 cut(s) 36, 1169
BslI CCNNNNNNNGG 5 cut(s) 283, 848, 899, 974, 1557
BsmAI GTCTC 6 cut(s) 76, 157, 327, 601, 874, 908
BsmFI GGGAC 2 cut(s) 36, 1169
BsnI GGCC 4 cut(s) 291, 1477, 1550, 1557
Bso31I GGTCTC 2 cut(s) 601, 908
BsoBI CYCGRG 2 cut(s) 551, 1013
Bsp1286I GDGCHC 2 cut(s) 1183, 1531
Bsp68I TCGCGA 2 cut(s) 60, 513
BspACI CCGC 5 cut(s) 38, 162, 918, 1206, 1300
BspANI GGCC 4 cut(s) 291, 1477, 1550, 1557
BspCNI CTCAG 3 cut(s) 520, 745, 850
BspDI ATCGAT 1 cut(s) 720
BspFNI CGCG 2 cut(s) 60, 513
BspLI GGNNCC 1 cut(s) 115
BspMAI CTGCAG 2 cut(s) 1111, 1234
BspMI ACCTGC 1 cut(s) 1575
BspPI GGATC 2 cut(s) 147, 995
BspQI GCTCTTC 2 cut(s) 668, 927
BspTNI GGTCTC 2 cut(s) 601, 908
BsrBI CCGCTC 1 cut(s) 920
BsrDI GCAATG 1 cut(s) 685
BsrFI RCCGGY 1 cut(s) 1557
BsrI ACTGG 4 cut(s) 880, 1043, 1283, 1491
BssAI RCCGGY 1 cut(s) 1557
BssECI CCNNGG 4 cut(s) 552, 687, 841, 1461
BssNI GRCGYC 1 cut(s) 172
BssT1I CCWWGG 2 cut(s) 687, 1461
Bst2UI CCWGG 1 cut(s) 843
Bst4CI ACNGT 1 cut(s) 455
Bst6I CTCTTC 4 cut(s) 668, 927, 1092, 1258
BstACI GRCGYC 1 cut(s) 172
BstAPI GCANNNNNTGC 1 cut(s) 1364
BstC8I GCNNGC 5 cut(s) 102, 620, 949, 1026, 1559
BstDEI CTNAG 3 cut(s) 528, 753, 858
BstENI CCTNNNNNAGG 1 cut(s) 846
BstF5I GGATG 4 cut(s) 34, 339, 505, 1241
BstFNI CGCG 2 cut(s) 60, 513
BstMAI GTCTC 6 cut(s) 76, 157, 327, 601, 874, 908
BstMCI CGRYCG 1 cut(s) 274
BstNI CCWGG 1 cut(s) 843
BstSCI CCNGG 3 cut(s) 19, 352, 841
BstSFI CTRYAG 3 cut(s) 1107, 1200, 1230
BstUI CGCG 2 cut(s) 60, 513
BstV1I GCAGC 9 cut(s) 987, 1072, 1155, 1208, 1210, 1213, 1216, 1244, 1247
BstV2I GAAGAC 3 cut(s) 243, 624, 1624
BstXI CCANNNNNNTGG 1 cut(s) 1336
Bsu15I ATCGAT 1 cut(s) 720
BsuRI GGCC 4 cut(s) 291, 1477, 1550, 1557
BsuTUI ATCGAT 1 cut(s) 720
BtgZI GCGATG 1 cut(s) 75
BtsCI GGATG 4 cut(s) 34, 339, 505, 1241
BtsIMutI CAGTG 4 cut(s) 451, 903, 1036, 1290
BtuMI TCGCGA 2 cut(s) 60, 513
BveI ACCTGC 1 cut(s) 1575
Cac8I GCNNGC 5 cut(s) 102, 620, 949, 1026, 1559
CaiI CAGNNNCTG 2 cut(s) 364, 527
Cfr10I RCCGGY 1 cut(s) 1557
Cfr13I GGNCC 3 cut(s) 113, 314, 1475
ClaI ATCGAT 1 cut(s) 720
CseI GACGC 1 cut(s) 1006
Csp6I GTAC 2 cut(s) 846, 1505
CviQI GTAC 2 cut(s) 846, 1505
DdeI CTNAG 3 cut(s) 528, 753, 858
EaeI YGGCCR 3 cut(s) 289, 1548, 1555
Eam1104I CTCTTC 4 cut(s) 668, 927, 1092, 1258
EarI CTCTTC 4 cut(s) 668, 927, 1092, 1258
Ecl136II GAGCTC 2 cut(s) 1181, 1529
Eco130I CCWWGG 2 cut(s) 687, 1461
Eco24I GRGCYC 2 cut(s) 1183, 1531
Eco31I GGTCTC 2 cut(s) 601, 908
Eco32I GATATC 2 cut(s) 540, 1314
Eco47I GGWCC 2 cut(s) 113, 314
Eco53kI GAGCTC 2 cut(s) 1181, 1529
Eco57I CTGAAG 2 cut(s) 255, 1116
Eco88I CYCGRG 2 cut(s) 551, 1013
EcoICRI GAGCTC 2 cut(s) 1181, 1529
EcoNI CCTNNNNNAGG 1 cut(s) 846
EcoRII CCWGG 1 cut(s) 841
EcoRV GATATC 2 cut(s) 540, 1314
EcoT14I CCWWGG 2 cut(s) 687, 1461
EcoT22I ATGCAT 1 cut(s) 1127
EcoT38I GRGCYC 2 cut(s) 1183, 1531
ErhI CCWWGG 2 cut(s) 687, 1461
FaqI GGGAC 2 cut(s) 36, 1169
FauNDI CATATG 1 cut(s) 562
FbaI TGATCA 3 cut(s) 223, 418, 610
FokI GGATG 4 cut(s) 41, 346, 512, 1228
FriOI GRGCYC 2 cut(s) 1183, 1531
FspBI CTAG 4 cut(s) 300, 926, 1070, 1630
GsuI CTGGAG 2 cut(s) 1266, 1446
HaeIII GGCC 4 cut(s) 291, 1477, 1550, 1557
HapII CCGG 3 cut(s) 20, 354, 1558
HgaI GACGC 1 cut(s) 1006
Hin1I GRCGYC 1 cut(s) 172
HinfI GANTC 8 cut(s) 43, 572, 647, 662, 860, 1294, 1325, 1390
HpaII CCGG 3 cut(s) 20, 354, 1558
HphI GGTGA 3 cut(s) 52, 591, 1289
Hpy188I TCNGA 7 cut(s) 370, 439, 615, 711, 790, 913, 960
Hpy99I CGWCG 1 cut(s) 1000
HpyAV CCTTC 5 cut(s) 277, 509, 1169, 1275, 1611
HpyCH4III ACNGT 1 cut(s) 455
HpyCH4IV ACGT 1 cut(s) 172
HpyF3I CTNAG 3 cut(s) 528, 753, 858
HpySE526I ACGT 1 cut(s) 172
Hsp92I GRCGYC 1 cut(s) 172
KroI GCCGGC 1 cut(s) 1557
KroNI GCCGGC 1 cut(s) 1559
Ksp22I TGATCA 3 cut(s) 223, 418, 610
LguI GCTCTTC 2 cut(s) 668, 927
LmnI GCTCC 4 cut(s) 109, 1178, 1526, 1534
Lsp1109I GCAGC 9 cut(s) 987, 1072, 1155, 1208, 1210, 1213, 1216, 1244, 1247
LweI GCATC 5 cut(s) 324, 430, 1011, 1134, 1247
MaeI CTAG 4 cut(s) 300, 926, 1070, 1630
MaeII ACGT 1 cut(s) 172
MaeIII GTNAC 6 cut(s) 263, 457, 587, 1276, 1339, 1441
MbiI CCGCTC 1 cut(s) 920
MfeI CAATTG 1 cut(s) 309
MhlI GDGCHC 2 cut(s) 1183, 1531
MlsI TGGCCA 2 cut(s) 291, 1550
MluNI TGGCCA 2 cut(s) 291, 1550
MlyI GAGTC 4 cut(s) 52, 581, 869, 1288
MmeI TCCRAC 3 cut(s) 148, 260, 711
Mox20I TGGCCA 2 cut(s) 291, 1550
Mph1103I ATGCAT 1 cut(s) 1127
MroNI GCCGGC 1 cut(s) 1557
MscI TGGCCA 2 cut(s) 291, 1550
MseI TTAA 9 cut(s) 8, 468, 579, 653, 746, 1304, 1400, 1512, 1625
MslI CAYNNNNRTG 3 cut(s) 252, 885, 1041
Msp20I TGGCCA 2 cut(s) 291, 1550
MspA1I CMGCKG 3 cut(s) 162, 971, 1168
MspI CCGG 3 cut(s) 20, 354, 1558
MspR9I CCNGG 3 cut(s) 21, 354, 843
MunI CAATTG 1 cut(s) 309
MvaI CCWGG 1 cut(s) 843
MvnI CGCG 2 cut(s) 60, 513
NaeI GCCGGC 1 cut(s) 1559
NciI CCSGG 2 cut(s) 21, 354
NdeI CATATG 1 cut(s) 562
NgoMIV GCCGGC 1 cut(s) 1557
NlaIV GGNNCC 1 cut(s) 115
NruI TCGCGA 2 cut(s) 60, 513
NsiI ATGCAT 1 cut(s) 1127
PciSI GCTCTTC 2 cut(s) 668, 927
PdiI GCCGGC 1 cut(s) 1559
PfeI GAWTC 4 cut(s) 647, 662, 1325, 1390
PflMI CCANNNNNTGG 1 cut(s) 974
Ple19I CGATCG 1 cut(s) 274
PleI GAGTC 4 cut(s) 51, 580, 868, 1288
PpsI GAGTC 4 cut(s) 51, 580, 868, 1288
PshBI ATTAAT 1 cut(s) 579
Psp124BI GAGCTC 2 cut(s) 1183, 1531
Psp6I CCWGG 1 cut(s) 841
PspGI CCWGG 1 cut(s) 841
PspN4I GGNNCC 1 cut(s) 115
PspPI GGNCC 3 cut(s) 113, 314, 1475
PstI CTGCAG 2 cut(s) 1111, 1234
PstNI CAGNNNCTG 2 cut(s) 364, 527
PvuI CGATCG 1 cut(s) 274
PvuII CAGCTG 2 cut(s) 971, 1168
RgaI GCGATCGC 1 cut(s) 274
RruI TCGCGA 2 cut(s) 60, 513
RsaI GTAC 2 cut(s) 847, 1506
RsaNI GTAC 2 cut(s) 846, 1505
RseI CAYNNNNRTG 3 cut(s) 252, 885, 1041
SacI GAGCTC 2 cut(s) 1183, 1531
SapI GCTCTTC 2 cut(s) 668, 927
SaqAI TTAA 9 cut(s) 8, 468, 579, 653, 746, 1304, 1400, 1512, 1625
Sau96I GGNCC 3 cut(s) 113, 314, 1475
SchI GAGTC 4 cut(s) 52, 581, 869, 1288
ScrFI CCNGG 3 cut(s) 21, 354, 843
SduI GDGCHC 2 cut(s) 1183, 1531
SfaAI GCGATCGC 1 cut(s) 274
SfaNI GCATC 5 cut(s) 324, 430, 1011, 1134, 1247
SfcI CTRYAG 3 cut(s) 1107, 1200, 1230
SgfI GCGATCGC 1 cut(s) 274
SinI GGWCC 2 cut(s) 113, 314
SmiMI CAYNNNNRTG 3 cut(s) 252, 885, 1041
SmlI CTYRAG 3 cut(s) 569, 668, 1182
SmoI CTYRAG 3 cut(s) 569, 668, 1182
SsiI CCGC 5 cut(s) 38, 162, 918, 1206, 1300
SspMI CTAG 4 cut(s) 300, 926, 1070, 1630
SstI GAGCTC 2 cut(s) 1183, 1531
StyD4I CCNGG 3 cut(s) 19, 352, 841
StyI CCWWGG 2 cut(s) 687, 1461
TaaI ACNGT 1 cut(s) 455
TaiI ACGT 1 cut(s) 175
TaqI TCGA 4 cut(s) 46, 720, 995, 1436
TatI WGTACW 1 cut(s) 1504
TauI GCSGC 1 cut(s) 1208
TfiI GAWTC 4 cut(s) 647, 662, 1325, 1390
Tru1I TTAA 9 cut(s) 8, 468, 579, 653, 746, 1304, 1400, 1512, 1625
Tru9I TTAA 9 cut(s) 8, 468, 579, 653, 746, 1304, 1400, 1512, 1625
TscAI CASTG 4 cut(s) 458, 903, 1043, 1290
TseI GCWGC 9 cut(s) 1000, 1085, 1168, 1196, 1223, 1226, 1229, 1232, 1235
TspDTI ATGAA 6 cut(s) 473, 696, 834, 945, 1245, 1484
TspGWI ACGGA 1 cut(s) 750
TspRI CASTG 4 cut(s) 458, 903, 1043, 1290
Van91I CCANNNNNTGG 1 cut(s) 974
VpaK11BI GGWCC 2 cut(s) 113, 314
VspI ATTAAT 1 cut(s) 579
XagI CCTNNNNNAGG 1 cut(s) 846
XapI RAATTY 2 cut(s) 507, 763
XbaI TCTAGA 1 cut(s) 925
XspI CTAG 4 cut(s) 300, 926, 1070, 1630
ZraI GACGTC 1 cut(s) 173
Zsp2I ATGCAT 1 cut(s) 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.