Rroxscaffold_4G00277980

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002123
Physical Location & Seq
Reverse (-)
1155018 .. 1156541
1524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00277980.1

Sequence Viewer

Length: 1074 bp
ATGACGACGACGACTCCCCGAAGGCAGATGACGCCGGAACAAGTCTTTCCGGCCACCGAGGACTCTTCGTTCATGACGAGGACGACTCCCTGGGCCAGACGACGCCGGAAAGCCGGCAAGAGGCCACCGAGGACTCTTCTTCCATTCCGAAAAATGCCTCCAATCCGATTGCTGCTTGAAGACTACACTGTCGTTGCTGCTCTGCCGTTTCACCGCATTCCCAAACGGCTTCGCCGTCCACTTTTCGGCACTCTCGCTCTTAGTAGAATAAGCTGCAGGATTCAACTTGATCAGATGGACACTCTTGCCGCAATTACGAACCAACGCTTTTGCCGTCGTCTGGCCTCGGGTACTCGGCGTCTTCCCGTTGTCGGCTCAGCAGGACGACGACGAGTCCCCGAGGCAGATGACGGCGAAGCGCTGGTTTGTAATCGCGGCGAGAGTAGCAGAGTGCATATGATCAAGTCGAATCCAGCAGCTTATTCTATACTGCCGAATAGGCTCGGGAAGATGCTTGCACCTTTACACTGGCATGATTACAAGAAAAAGTATGGAAAGCTAGATGATTTCGTTGCTGCTCATACTGAACTATTTGTGATTGAGGGGGACTATATTCAGCTGCGTGAAGGAGCTCAAGAGATGATAGCAGCTACAGCCGCCGTTGCCAGAGTTGCTGCTGCTGCAGCAGCAGCATCCCCCTACTCTTCATGTTTGCCTTCTGTGGCTGTTACTCCAGTGGCACAGACTCACCGCTTAAAGAAGATATCATCTCTTGATTCCAAAAATGTGGTTACTTCTACTCCAAATGCAAATAATGCTCACTTGCAGTCTGTGAAGCAGAACCAGCAATTAAATGGTGTATCTTTTGGTGTTTCGGGTATGTCGAACGTAAAAATTTTGAGCAAATCCAAGGACATGAATGGGCCTGAACCTACTCCCAGCCAGTCATCTGTACTTCTTAATGGTGGAAATGGTGCTCCTCTGGACAGATCAATGGCCAATGGAAGGCCGAGCTCAAATTTTGTTGGGAAACAGCATGGCAGTCAAGTCTCCACAGCCAGCTGTCTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

357

Amino Acids

39.06

Weight (kDa)

10.67

Isoelectric Point (pI)

58.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7725 PF24851 161 - 208 2.6e-24 Domain of unknown function (DUF7725)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
AccII CGCG 1 cut(s) 435
AciI CCGC 5 cut(s) 214, 309, 435, 657, 751
AcoI YGGCCR 2 cut(s) 51, 996
AcsI RAATTY 2 cut(s) 894, 1018
AcyI GRCGYC 3 cut(s) 32, 103, 358
AfaI GTAC 2 cut(s) 352, 954
AfeI AGCGCT 1 cut(s) 420
AfiI CCNNNNNNNGG 5 cut(s) 120, 245, 340, 371, 1005
AgsI TTSAA 2 cut(s) 179, 284
AhdI GACNNNNNGTC 1 cut(s) 392
AjnI CCWGG 1 cut(s) 89
AloI GAACNNNNNNTCC 2 cut(s) 53, 85
AluBI AGCT 8 cut(s) 273, 479, 559, 619, 632, 650, 1014, 1062
AluI AGCT 8 cut(s) 273, 479, 559, 619, 632, 650, 1014, 1062
Alw21I GWGCWC 3 cut(s) 634, 979, 1016
Alw26I GTCTC 1 cut(s) 1054
Ama87I CYCGRG 3 cut(s) 346, 398, 503
Aor51HI AGCGCT 1 cut(s) 420
AoxI GGCC 7 cut(s) 51, 93, 122, 342, 923, 996, 1007
ApoI RAATTY 2 cut(s) 894, 1018
AspLEI GCGC 1 cut(s) 421
AspS9I GGNCC 2 cut(s) 93, 923
AsuHPI GGTGA 2 cut(s) 203, 740
AvaI CYCGRG 3 cut(s) 346, 398, 503
BalI TGGCCA 1 cut(s) 998
BanII GRGCYC 2 cut(s) 634, 1016
BbsI GAAGAC 2 cut(s) 186, 353
Bbv12I GWGCWC 3 cut(s) 634, 979, 1016
BccI CCATC 1 cut(s) 289
BceAI ACGGC 6 cut(s) 190, 219, 242, 318, 427, 644
BciT130I CCWGG 1 cut(s) 91
BclI TGATCA 2 cut(s) 289, 459
BcoDI GTCTC 1 cut(s) 1054
BfaI CTAG 1 cut(s) 560
BfmI CTRYAG 3 cut(s) 274, 651, 681
BfoI RGCGCY 1 cut(s) 422
BglI GCCNNNNNGGC 1 cut(s) 499
BlpI GCTNAGC 1 cut(s) 376
Bme1390I CCNGG 1 cut(s) 91
BmeRI GACNNNNNGTC 1 cut(s) 392
BmeT110I CYCGRG 3 cut(s) 346, 398, 503
BmgT120I GGNCC 2 cut(s) 93, 923
BmrFI CCNGG 1 cut(s) 91
BmsI GCATC 2 cut(s) 501, 701
BpiI GAAGAC 2 cut(s) 186, 353
BplI GAGNNNNNCTC 2 cut(s) 70, 102
BpmI CTGGAG 1 cut(s) 717
Bpu1102I GCTNAGC 1 cut(s) 376
BpuEI CTTGAG 1 cut(s) 618
BsaHI GRCGYC 3 cut(s) 32, 103, 358
BsaJI CCNNGG 7 cut(s) 57, 89, 90, 128, 345, 399, 909
BsaXI ACNNNNNCTCC 3 cut(s) 28, 784, 814
Bsc4I CCNNNNNNNGG 5 cut(s) 120, 245, 340, 371, 1005
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 3 cut(s) 533, 734, 943
BseBI CCWGG 1 cut(s) 91
BseDI CCNNGG 7 cut(s) 57, 89, 90, 128, 345, 399, 909
BseGI GGATG 1 cut(s) 692
BseLI CCNNNNNNNGG 5 cut(s) 120, 245, 340, 371, 1005
BseMII CTCAG 1 cut(s) 390
BseNI ACTGG 3 cut(s) 533, 734, 943
BseRI GAGGAG 1 cut(s) 969
BseYI CCCAGC 1 cut(s) 938
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 7 cut(s) 53, 95, 124, 344, 925, 998, 1009
BsiHKAI GWGCWC 3 cut(s) 634, 979, 1016
BsiHKCI CYCGRG 3 cut(s) 346, 398, 503
BsiSI CCGG 4 cut(s) 35, 50, 106, 114
BslFI GGGAC 2 cut(s) 380, 620
BslI CCNNNNNNNGG 5 cut(s) 120, 245, 340, 371, 1005
BsmAI GTCTC 1 cut(s) 1054
BsmFI GGGAC 2 cut(s) 380, 620
BsmI GAATGC 1 cut(s) 216
BsnI GGCC 7 cut(s) 53, 95, 124, 344, 925, 998, 1009
BsoBI CYCGRG 3 cut(s) 346, 398, 503
Bsp1286I GDGCHC 3 cut(s) 634, 979, 1016
Bsp143I GATC 3 cut(s) 289, 459, 989
Bsp1720I GCTNAGC 1 cut(s) 376
BspACI CCGC 5 cut(s) 214, 309, 435, 657, 751
BspANI GGCC 7 cut(s) 53, 95, 124, 344, 925, 998, 1009
BspCNI CTCAG 1 cut(s) 389
BspFNI CGCG 1 cut(s) 435
BspHI TCATGA 1 cut(s) 72
BspMAI CTGCAG 2 cut(s) 278, 685
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 3 cut(s) 533, 734, 943
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 7 cut(s) 57, 89, 90, 128, 345, 399, 909
BssMI GATC 3 cut(s) 289, 459, 989
BssNI GRCGYC 3 cut(s) 32, 103, 358
BssT1I CCWWGG 1 cut(s) 909
Bst2UI CCWGG 1 cut(s) 91
Bst4CI ACNGT 1 cut(s) 190
Bst6I CTCTTC 3 cut(s) 70, 141, 709
BstACI GRCGYC 3 cut(s) 32, 103, 358
BstAPI GCANNNNNTGC 1 cut(s) 815
BstC8I GCNNGC 3 cut(s) 115, 516, 1060
BstDEI CTNAG 2 cut(s) 260, 376
BstF5I GGATG 1 cut(s) 692
BstFNI CGCG 1 cut(s) 435
BstH2I RGCGCY 1 cut(s) 422
BstHHI GCGC 1 cut(s) 421
BstKTI GATC 3 cut(s) 292, 462, 992
BstMAI GTCTC 1 cut(s) 1054
BstMBI GATC 3 cut(s) 289, 459, 989
BstNI CCWGG 1 cut(s) 91
BstSCI CCNGG 1 cut(s) 89
BstSFI CTRYAG 3 cut(s) 274, 651, 681
BstUI CGCG 1 cut(s) 435
BstV2I GAAGAC 2 cut(s) 186, 353
BstXI CCANNNNNNTGG 1 cut(s) 787
BsuRI GGCC 7 cut(s) 53, 95, 124, 344, 925, 998, 1009
BtsCI GGATG 1 cut(s) 692
BtsIMutI CAGTG 3 cut(s) 186, 526, 741
Cac8I GCNNGC 3 cut(s) 115, 516, 1060
CciI TCATGA 1 cut(s) 72
CfoI GCGC 1 cut(s) 421
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 2 cut(s) 93, 923
CseI GACGC 3 cut(s) 40, 111, 347
Csp6I GTAC 2 cut(s) 351, 953
CviAII CATG 5 cut(s) 73, 533, 708, 916, 1037
CviQI GTAC 2 cut(s) 351, 953
DdeI CTNAG 2 cut(s) 260, 376
DpnI GATC 3 cut(s) 291, 461, 991
DpnII GATC 3 cut(s) 289, 459, 989
DrdI GACNNNNNNGTC 1 cut(s) 188
DriI GACNNNNNGTC 1 cut(s) 392
DseDI GACNNNNNNGTC 1 cut(s) 188
EaeI YGGCCR 2 cut(s) 51, 996
Eam1104I CTCTTC 3 cut(s) 70, 141, 709
Eam1105I GACNNNNNGTC 1 cut(s) 392
EarI CTCTTC 3 cut(s) 70, 141, 709
Ecl136II GAGCTC 2 cut(s) 632, 1014
Eco130I CCWWGG 1 cut(s) 909
Eco24I GRGCYC 2 cut(s) 634, 1016
Eco32I GATATC 1 cut(s) 765
Eco47III AGCGCT 1 cut(s) 420
Eco53kI GAGCTC 2 cut(s) 632, 1014
Eco88I CYCGRG 3 cut(s) 346, 398, 503
EcoICRI GAGCTC 2 cut(s) 632, 1014
EcoRII CCWGG 1 cut(s) 89
EcoRV GATATC 1 cut(s) 765
EcoT14I CCWWGG 1 cut(s) 909
EcoT38I GRGCYC 2 cut(s) 634, 1016
ErhI CCWWGG 1 cut(s) 909
FaeI CATG 5 cut(s) 76, 536, 711, 919, 1040
FaqI GGGAC 2 cut(s) 380, 620
FatI CATG 5 cut(s) 72, 532, 707, 915, 1036
FauNDI CATATG 1 cut(s) 456
FbaI TGATCA 2 cut(s) 289, 459
FokI GGATG 1 cut(s) 679
FriOI GRGCYC 2 cut(s) 634, 1016
FspBI CTAG 1 cut(s) 560
GlaI GCGC 1 cut(s) 420
GsaI CCCAGC 1 cut(s) 942
GsuI CTGGAG 1 cut(s) 717
HaeII RGCGCY 1 cut(s) 422
HaeIII GGCC 7 cut(s) 53, 95, 124, 344, 925, 998, 1009
HapII CCGG 4 cut(s) 35, 50, 106, 114
HgaI GACGC 3 cut(s) 40, 111, 347
HhaI GCGC 1 cut(s) 421
Hin1I GRCGYC 3 cut(s) 32, 103, 358
Hin1II CATG 5 cut(s) 76, 536, 711, 919, 1040
Hin6I GCGC 1 cut(s) 419
HinP1I GCGC 1 cut(s) 419
HinfI GANTC 9 cut(s) 13, 62, 85, 133, 280, 393, 469, 745, 776
HpaII CCGG 4 cut(s) 35, 50, 106, 114
HphI GGTGA 2 cut(s) 203, 740
Hpy166II GTNNAC 1 cut(s) 239
Hpy188I TCNGA 4 cut(s) 149, 167, 294, 1068
Hpy188III TCNNGA 5 cut(s) 73, 505, 635, 773, 983
Hpy8I GTNNAC 1 cut(s) 239
Hpy99I CGWCG 6 cut(s) 10, 13, 105, 339, 390, 393
HpyAV CCTTC 4 cut(s) 15, 620, 726, 999
HpyCH4III ACNGT 1 cut(s) 190
HpyCH4IV ACGT 1 cut(s) 888
HpyCH4V TGCA 6 cut(s) 276, 454, 518, 683, 809, 826
HpyF3I CTNAG 2 cut(s) 260, 376
HpySE526I ACGT 1 cut(s) 888
Hsp92I GRCGYC 3 cut(s) 32, 103, 358
Hsp92II CATG 5 cut(s) 76, 536, 711, 919, 1040
HspAI GCGC 1 cut(s) 419
KroI GCCGGC 1 cut(s) 113
KroNI GCCGGC 1 cut(s) 115
Ksp22I TGATCA 2 cut(s) 289, 459
Kzo9I GATC 3 cut(s) 289, 459, 989
LmnI GCTCC 2 cut(s) 629, 982
LweI GCATC 2 cut(s) 501, 701
MaeI CTAG 1 cut(s) 560
MaeII ACGT 1 cut(s) 888
MaeIII GTNAC 2 cut(s) 727, 790
MalI GATC 3 cut(s) 291, 461, 991
MboI GATC 3 cut(s) 289, 459, 989
MboII GAAGA 8 cut(s) 57, 128, 131, 191, 353, 520, 696, 772
MhlI GDGCHC 3 cut(s) 634, 979, 1016
MlsI TGGCCA 1 cut(s) 998
MluCI AATT 4 cut(s) 312, 848, 894, 1018
MluNI TGGCCA 1 cut(s) 998
MlyI GAGTC 6 cut(s) 7, 56, 79, 127, 402, 739
MnlI CCTC 9 cut(s) 52, 72, 114, 123, 168, 355, 394, 595, 990
Mox20I TGGCCA 1 cut(s) 998
MroNI GCCGGC 1 cut(s) 113
MscI TGGCCA 1 cut(s) 998
MseI TTAA 3 cut(s) 755, 851, 960
MslI CAYNNNNRTG 1 cut(s) 531
Msp20I TGGCCA 1 cut(s) 998
MspA1I CMGCKG 2 cut(s) 619, 1062
MspI CCGG 4 cut(s) 35, 50, 106, 114
MspR9I CCNGG 1 cut(s) 91
Mva1269I GAATGC 1 cut(s) 216
MvaI CCWGG 1 cut(s) 91
MvnI CGCG 1 cut(s) 435
NaeI GCCGGC 1 cut(s) 115
NdeI CATATG 1 cut(s) 456
NdeII GATC 3 cut(s) 289, 459, 989
NgoMIV GCCGGC 1 cut(s) 113
NlaIII CATG 5 cut(s) 76, 536, 711, 919, 1040
NmeAIII GCCGAG 2 cut(s) 334, 1035
PagI TCATGA 1 cut(s) 72
PasI CCCWGGG 1 cut(s) 90
PcsI WCGNNNNNNNCGW 4 cut(s) 74, 232, 331, 881
PctI GAATGC 1 cut(s) 216
PdiI GCCGGC 1 cut(s) 115
PfeI GAWTC 3 cut(s) 280, 469, 776
PleI GAGTC 6 cut(s) 7, 56, 79, 127, 401, 739
PpsI GAGTC 6 cut(s) 7, 56, 79, 127, 401, 739
Psp124BI GAGCTC 2 cut(s) 634, 1016
Psp6I CCWGG 1 cut(s) 89
PspFI CCCAGC 1 cut(s) 938
PspGI CCWGG 1 cut(s) 89
PspPI GGNCC 2 cut(s) 93, 923
PstI CTGCAG 2 cut(s) 278, 685
PvuII CAGCTG 2 cut(s) 619, 1062
RsaI GTAC 2 cut(s) 352, 954
RsaNI GTAC 2 cut(s) 351, 953
RseI CAYNNNNRTG 1 cut(s) 531
SacI GAGCTC 2 cut(s) 634, 1016
SaqAI TTAA 3 cut(s) 755, 851, 960
Sau3AI GATC 3 cut(s) 289, 459, 989
Sau96I GGNCC 2 cut(s) 93, 923
SchI GAGTC 6 cut(s) 7, 56, 79, 127, 402, 739
ScrFI CCNGG 1 cut(s) 91
SduI GDGCHC 3 cut(s) 634, 979, 1016
SfaNI GCATC 2 cut(s) 501, 701
SfcI CTRYAG 3 cut(s) 274, 651, 681
SmiMI CAYNNNNRTG 1 cut(s) 531
SmlI CTYRAG 1 cut(s) 633
SmoI CTYRAG 1 cut(s) 633
Sse9I AATT 4 cut(s) 312, 848, 894, 1018
SsiI CCGC 5 cut(s) 214, 309, 435, 657, 751
SspMI CTAG 1 cut(s) 560
SstI GAGCTC 2 cut(s) 634, 1016
StyD4I CCNGG 1 cut(s) 89
StyI CCWWGG 1 cut(s) 909
TaaI ACNGT 1 cut(s) 190
TaiI ACGT 1 cut(s) 891
TaqI TCGA 2 cut(s) 467, 884
TasI AATT 4 cut(s) 312, 848, 894, 1018
TatI WGTACW 1 cut(s) 952
TauI GCSGC 3 cut(s) 311, 438, 659
TfiI GAWTC 3 cut(s) 280, 469, 776
Tru1I TTAA 3 cut(s) 755, 851, 960
Tru9I TTAA 3 cut(s) 755, 851, 960
TscAI CASTG 3 cut(s) 193, 533, 741
TspDTI ATGAA 3 cut(s) 61, 696, 932
TspRI CASTG 3 cut(s) 193, 533, 741
XapI RAATTY 2 cut(s) 894, 1018
XcmI CCANNNNNNNNNTGG 1 cut(s) 851
XspI CTAG 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.