RchiOBHm_Chr4g0412311

histone-lysine N-methyltransferase, H3 lysine-9 specific

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001110
Physical Location & Seq
Reverse (-)
36037271 .. 36038434
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38299

Sequence Viewer

Length: 1164 bp
ATGTCATCAACGGATGCGAAGAAAGACGGGCGAAAGAAAGCCAAGGTTGATGGTGCTACAGAGACTTTGGAGGTAAACAATTTAGGGATTAGTTGGTTTGCTCCATTTACTGTTTTAAGCACGAGCTCTATTAGCTTTTTTAGAAGCTTGAGAGAAAATCTTAGAGTTGACAATCTAGTCAGTACTGATCAGTTCCATTTTACTCGTAAGAGTCAACTGGCTATAGGTCAAGAGTCTTCTAGGGTTGTTGAGAATGACTTACCCGGGTTGGTCTGCAACGACCTTTGTAATGGAAAAGAAAACATATGCATCCCTGTTACAAATGAAATTGATGTCCCTCCTGTAGCTCCCGAAAAGTTTGAGTATCTCAGCTCCATTGAAGTAGCAAAAAATTTCGTCATTCCGGCGAGTGCTGAGGGATGTAATTGCAAAGGGAAGTGTACTAATACGAGGACTTGTTCGTGTGCTAGGCTCAATGGATCTGACTTTCCTTATGTTCGGAAAGCCGGTGGCGGCAGATTGTTTGAGCCTAAGGCTGTTGTTTTCGAGTGTGGTCCGAACTGTGGCTGCGGGCCCAAGTGTGTGAACCGTACTTCTCAGCAGGGATTGAAGTACAGGCTTGAGGTTTATAAGACACCTGACAAAGGCTGGGCTGTTAGGTCTTGGGATTTTATTCCCTCTGGTGGTTATGTTTGCGAGTATACTGGTGTTCTGATGAAGACCAGTGAACTGGACAATGTCTCCATGAATGACTACTTTTTTGAAATTGATTGTGAGCATACAATGAATGGGATTGGAGTGAGGGAGAAGCGCCTAGGTGAAGTGCCCATTTCAAATGGTCTTGGTGCTGGCGAAGATGGAGGTGACAAGTTATTAGTAGAAAGTGACCCCGACTATTGTATAGATGCTGGATCCAGTGGGAATGTTGCTAGGTTTATCAACCATAGTTGCGAGCCGAATCTGTTTGTGCAGTGTGTCTTGAGTTCTCACCATGATGTTAAACTAGCTAGAATTGTGTTGTTTGCTTCCGATGACATACCTCCTATGCAAGAGCTGACTTATGACTATGGCTATGAGCTTGATAGTGTCGTTGGTGCTGATGGAAAAGTCAAGGAGTTGGCTTGTCATTGTGGTACAGCGGACTGTCGGAAGCGTTTGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SAD_SRA SET

Protein Analysis

387

Amino Acids

42.44

Weight (kDa)

5.35

Isoelectric Point (pI)

29.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pre-SET PF05033 97 - 197 6.7e-18 Pre-SET motif
SET PF00856 216 - 357 4.3e-20 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 630
AasI GACNNNNNNGTC 1 cut(s) 176
AccI GTMKAC 1 cut(s) 701
AciI CCGC 3 cut(s) 513, 570, 1139
AclWI GGATC 3 cut(s) 487, 906, 919
AcsI RAATTY 1 cut(s) 391
AfaI GTAC 6 cut(s) 184, 442, 592, 614, 1135, 1160
AfiI CCNNNNNNNGG 3 cut(s) 563, 644, 683
AgsI TTSAA 4 cut(s) 380, 610, 764, 834
AluBI AGCT 8 cut(s) 126, 135, 147, 347, 372, 1007, 1054, 1078
AluI AGCT 8 cut(s) 126, 135, 147, 347, 372, 1007, 1054, 1078
Alw21I GWGCWC 1 cut(s) 128
Alw26I GTCTC 2 cut(s) 56, 745
AlwI GGATC 3 cut(s) 487, 906, 919
Ama87I CYCGRG 1 cut(s) 263
AoxI GGCC 1 cut(s) 572
ApaI GGGCCC 1 cut(s) 576
ApeKI GCWGC 1 cut(s) 567
ApoI RAATTY 1 cut(s) 391
ArsI GACNNNNNNTTYG 2 cut(s) 743, 775
AspA2I CCTAGG 1 cut(s) 814
AspLEI GCGC 1 cut(s) 813
AspS9I GGNCC 3 cut(s) 554, 572, 573
AsuC2I CCSGG 2 cut(s) 264, 265
AsuHPI GGTGA 3 cut(s) 830, 875, 980
AvaI CYCGRG 1 cut(s) 263
AvaII GGWCC 1 cut(s) 554
AvrII CCTAGG 1 cut(s) 814
AxyI CCTNAGG 1 cut(s) 531
BaeGI GKGCMC 2 cut(s) 576, 828
BamHI GGATCC 1 cut(s) 911
BanII GRGCYC 2 cut(s) 128, 576
BarI GAAGNNNNNNTAC 1 cut(s) 1142
BauI CACGAG 1 cut(s) 121
BbsI GAAGAC 2 cut(s) 228, 725
Bbv12I GWGCWC 1 cut(s) 128
BbvCI CCTCAGC 1 cut(s) 414
BbvI GCAGC 1 cut(s) 554
BccI CCATC 3 cut(s) 44, 851, 1094
BclI TGATCA 1 cut(s) 187
BcnI CCSGG 2 cut(s) 264, 265
BcoDI GTCTC 2 cut(s) 56, 745
BfaI CTAG 8 cut(s) 176, 240, 468, 815, 930, 1004, 1008, 1162
BfmI CTRYAG 3 cut(s) 57, 222, 342
BfoI RGCGCY 1 cut(s) 814
BisI GCNGC 2 cut(s) 514, 568
BlnI CCTAGG 1 cut(s) 814
BlsI GCNGC 2 cut(s) 515, 569
BmcAI AGTACT 1 cut(s) 184
Bme1390I CCNGG 2 cut(s) 264, 265
Bme18I GGWCC 1 cut(s) 554
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 3 cut(s) 554, 572, 573
BmiI GGNNCC 2 cut(s) 574, 913
BmrFI CCNGG 2 cut(s) 264, 265
BmsI GCATC 3 cut(s) 4, 318, 895
BpiI GAAGAC 2 cut(s) 228, 725
Bpu10I CCTNAGC 1 cut(s) 414
BpuEI CTTGAG 3 cut(s) 169, 641, 1000
BpuMI CCSGG 2 cut(s) 264, 265
BsaJI CCNNGG 3 cut(s) 42, 263, 814
BsaXI ACNNNNNCTCC 2 cut(s) 725, 755
Bsc4I CCNNNNNNNGG 3 cut(s) 563, 644, 683
Bse118I RCCGGY 1 cut(s) 506
Bse1I ACTGG 5 cut(s) 222, 709, 723, 735, 915
Bse21I CCTNAGG 1 cut(s) 531
BseDI CCNNGG 3 cut(s) 42, 263, 814
BseGI GGATG 3 cut(s) 19, 309, 425
BseLI CCNNNNNNNGG 3 cut(s) 563, 644, 683
BseMII CTCAG 3 cut(s) 382, 405, 611
BseNI ACTGG 5 cut(s) 222, 709, 723, 735, 915
BseSI GKGCMC 2 cut(s) 576, 828
BseXI GCAGC 1 cut(s) 554
BseYI CCCAGC 1 cut(s) 648
BsgI GTGCAG 1 cut(s) 989
BshFI GGCC 1 cut(s) 574
BsiHKAI GWGCWC 1 cut(s) 128
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 3 cut(s) 264, 404, 507
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 3 cut(s) 563, 644, 683
BsmAI GTCTC 2 cut(s) 56, 745
BsmFI GGGAC 1 cut(s) 320
BsnI GGCC 1 cut(s) 574
BsoBI CYCGRG 1 cut(s) 263
Bsp120I GGGCCC 1 cut(s) 572
Bsp1286I GDGCHC 3 cut(s) 128, 576, 828
Bsp143I GATC 3 cut(s) 187, 479, 911
BspACI CCGC 3 cut(s) 513, 570, 1139
BspANI GGCC 1 cut(s) 574
BspCNI CTCAG 3 cut(s) 381, 406, 610
BspLI GGNNCC 2 cut(s) 574, 913
BspPI GGATC 3 cut(s) 487, 906, 919
BsrFI RCCGGY 1 cut(s) 506
BsrI ACTGG 5 cut(s) 222, 709, 723, 735, 915
BssAI RCCGGY 1 cut(s) 506
BssECI CCNNGG 3 cut(s) 42, 263, 814
BssMI GATC 3 cut(s) 187, 479, 911
BssNAI GTATAC 1 cut(s) 702
BssSI CACGAG 1 cut(s) 121
BssT1I CCWWGG 2 cut(s) 42, 814
Bst1107I GTATAC 1 cut(s) 702
Bst2BI CACGAG 1 cut(s) 121
Bst4CI ACNGT 4 cut(s) 112, 563, 590, 1145
BstC8I GCNNGC 3 cut(s) 572, 850, 953
BstDEI CTNAG 5 cut(s) 161, 368, 414, 531, 597
BstENI CCTNNNNNAGG 1 cut(s) 642
BstF5I GGATG 3 cut(s) 19, 309, 425
BstH2I RGCGCY 1 cut(s) 814
BstHHI GCGC 1 cut(s) 813
BstKTI GATC 3 cut(s) 190, 482, 914
BstMAI GTCTC 2 cut(s) 56, 745
BstMBI GATC 3 cut(s) 187, 479, 911
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstSCI CCNGG 2 cut(s) 262, 263
BstSFI CTRYAG 3 cut(s) 57, 222, 342
BstSLI GKGCMC 2 cut(s) 576, 828
BstV1I GCAGC 1 cut(s) 554
BstV2I GAAGAC 2 cut(s) 228, 725
BstX2I RGATCY 2 cut(s) 479, 911
BstXI CCANNNNNNTGG 1 cut(s) 730
BstYI RGATCY 2 cut(s) 479, 911
BstZ17I GTATAC 1 cut(s) 702
Bsu36I CCTNAGG 1 cut(s) 531
BsuRI GGCC 1 cut(s) 574
BtsCI GGATG 3 cut(s) 19, 309, 425
BtsI GCAGTG 1 cut(s) 977
BtsIMutI CAGTG 3 cut(s) 730, 922, 977
Cac8I GCNNGC 3 cut(s) 572, 850, 953
CfoI GCGC 1 cut(s) 813
Cfr10I RCCGGY 1 cut(s) 506
Cfr13I GGNCC 3 cut(s) 554, 572, 573
Cfr9I CCCGGG 1 cut(s) 263
Csp6I GTAC 6 cut(s) 183, 441, 591, 613, 1134, 1159
CviAII CATG 2 cut(s) 745, 992
CviQI GTAC 6 cut(s) 183, 441, 591, 613, 1134, 1159
DdeI CTNAG 5 cut(s) 161, 368, 414, 531, 597
DpnI GATC 3 cut(s) 189, 481, 913
DpnII GATC 3 cut(s) 187, 479, 911
DrdI GACNNNNNNGTC 1 cut(s) 176
DseDI GACNNNNNNGTC 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 126
Eco130I CCWWGG 2 cut(s) 42, 814
Eco24I GRGCYC 2 cut(s) 128, 576
Eco47I GGWCC 1 cut(s) 554
Eco53kI GAGCTC 1 cut(s) 126
Eco81I CCTNAGG 1 cut(s) 531
Eco88I CYCGRG 1 cut(s) 263
EcoICRI GAGCTC 1 cut(s) 126
EcoNI CCTNNNNNAGG 1 cut(s) 642
EcoT14I CCWWGG 2 cut(s) 42, 814
EcoT22I ATGCAT 1 cut(s) 311
EcoT38I GRGCYC 2 cut(s) 128, 576
ErhI CCWWGG 2 cut(s) 42, 814
FaeI CATG 2 cut(s) 748, 995
FaqI GGGAC 1 cut(s) 320
FatI CATG 2 cut(s) 744, 991
FauI CCCGC 1 cut(s) 563
FauNDI CATATG 1 cut(s) 305
FbaI TGATCA 1 cut(s) 187
FblI GTMKAC 1 cut(s) 701
Fnu4HI GCNGC 2 cut(s) 514, 568
FokI GGATG 3 cut(s) 26, 296, 432
FriOI GRGCYC 2 cut(s) 128, 576
Fsp4HI GCNGC 2 cut(s) 514, 568
FspBI CTAG 8 cut(s) 176, 240, 468, 815, 930, 1004, 1008, 1162
GlaI GCGC 1 cut(s) 812
GluI GCNGC 2 cut(s) 514, 568
GsaI CCCAGC 1 cut(s) 652
HaeII RGCGCY 1 cut(s) 814
HaeIII GGCC 1 cut(s) 574
HapII CCGG 3 cut(s) 264, 404, 507
HhaI GCGC 1 cut(s) 813
Hin1II CATG 2 cut(s) 748, 995
Hin6I GCGC 1 cut(s) 811
HinP1I GCGC 1 cut(s) 811
HincII GTYRAC 2 cut(s) 169, 215
HindII GTYRAC 2 cut(s) 169, 215
HindIII AAGCTT 1 cut(s) 145
HinfI GANTC 3 cut(s) 211, 233, 958
HpaII CCGG 3 cut(s) 264, 404, 507
HphI GGTGA 3 cut(s) 830, 875, 980
Hpy166II GTNNAC 7 cut(s) 76, 169, 215, 441, 586, 702, 728
Hpy188I TCNGA 6 cut(s) 484, 501, 558, 714, 1030, 1149
Hpy188III TCNNGA 3 cut(s) 230, 350, 979
Hpy8I GTNNAC 7 cut(s) 76, 169, 215, 441, 586, 702, 728
HpyCH4III ACNGT 4 cut(s) 112, 563, 590, 1145
HpyCH4V TGCA 5 cut(s) 276, 309, 429, 970, 1048
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
HpyF3I CTNAG 5 cut(s) 161, 368, 414, 531, 597
Hsp92II CATG 2 cut(s) 748, 995
HspAI GCGC 1 cut(s) 811
Ksp22I TGATCA 1 cut(s) 187
Kzo9I GATC 3 cut(s) 187, 479, 911
LmnI GCTCC 3 cut(s) 106, 352, 377
Lsp1109I GCAGC 1 cut(s) 554
LweI GCATC 3 cut(s) 4, 318, 895
MaeI CTAG 8 cut(s) 176, 240, 468, 815, 930, 1004, 1008, 1162
MaeIII GTNAC 3 cut(s) 316, 863, 884
MalI GATC 3 cut(s) 189, 481, 913
MboI GATC 3 cut(s) 187, 479, 911
MboII GAAGA 4 cut(s) 31, 228, 730, 866
MflI RGATCY 2 cut(s) 479, 911
MhlI GDGCHC 3 cut(s) 128, 576, 828
MluCI AATT 6 cut(s) 79, 327, 391, 424, 765, 1011
MlyI GAGTC 2 cut(s) 220, 242
MmeI TCCRAC 1 cut(s) 1127
MnlI CCTC 9 cut(s) 64, 348, 409, 444, 616, 688, 795, 854, 1050
Mph1103I ATGCAT 1 cut(s) 311
MseI TTAA 2 cut(s) 116, 999
MslI CAYNNNNRTG 1 cut(s) 993
MspA1I CMGCKG 1 cut(s) 1139
MspI CCGG 3 cut(s) 264, 404, 507
MspR9I CCNGG 2 cut(s) 264, 265
MwoI GCNNNNNNNGC 1 cut(s) 132
NciI CCSGG 2 cut(s) 264, 265
NdeI CATATG 1 cut(s) 305
NdeII GATC 3 cut(s) 187, 479, 911
NlaIII CATG 2 cut(s) 748, 995
NlaIV GGNNCC 2 cut(s) 574, 913
NmuCI GTSAC 2 cut(s) 863, 884
NsiI ATGCAT 1 cut(s) 311
PfeI GAWTC 1 cut(s) 958
PflFI GACNNNGTC 1 cut(s) 737
PkrI GCNGC 2 cut(s) 515, 569
PleI GAGTC 2 cut(s) 219, 241
PpsI GAGTC 2 cut(s) 219, 241
PsiI TTATAA 1 cut(s) 630
Psp124BI GAGCTC 1 cut(s) 128
PspFI CCCAGC 1 cut(s) 648
PspN4I GGNNCC 2 cut(s) 574, 913
PspOMI GGGCCC 1 cut(s) 572
PspPI GGNCC 3 cut(s) 554, 572, 573
PsuI RGATCY 2 cut(s) 479, 911
PsyI GACNNNGTC 1 cut(s) 737
RsaI GTAC 6 cut(s) 184, 442, 592, 614, 1135, 1160
RsaNI GTAC 6 cut(s) 183, 441, 591, 613, 1134, 1159
RseI CAYNNNNRTG 1 cut(s) 993
SacI GAGCTC 1 cut(s) 128
SaqAI TTAA 2 cut(s) 116, 999
SatI GCNGC 2 cut(s) 514, 568
Sau3AI GATC 3 cut(s) 187, 479, 911
Sau96I GGNCC 3 cut(s) 554, 572, 573
ScaI AGTACT 1 cut(s) 184
SchI GAGTC 2 cut(s) 220, 242
ScrFI CCNGG 2 cut(s) 264, 265
SduI GDGCHC 3 cut(s) 128, 576, 828
SfaNI GCATC 3 cut(s) 4, 318, 895
SfcI CTRYAG 3 cut(s) 57, 222, 342
SinI GGWCC 1 cut(s) 554
SmaI CCCGGG 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 993
SmlI CTYRAG 3 cut(s) 148, 620, 979
SmoI CTYRAG 3 cut(s) 148, 620, 979
Sse9I AATT 6 cut(s) 79, 327, 391, 424, 765, 1011
SsiI CCGC 3 cut(s) 513, 570, 1139
SspMI CTAG 8 cut(s) 176, 240, 468, 815, 930, 1004, 1008, 1162
SstI GAGCTC 1 cut(s) 128
StyD4I CCNGG 2 cut(s) 262, 263
StyI CCWWGG 2 cut(s) 42, 814
TaaI ACNGT 4 cut(s) 112, 563, 590, 1145
TaqI TCGA 1 cut(s) 546
TasI AATT 6 cut(s) 79, 327, 391, 424, 765, 1011
TatI WGTACW 4 cut(s) 182, 440, 612, 1158
TauI GCSGC 1 cut(s) 516
TfiI GAWTC 1 cut(s) 958
Tru1I TTAA 2 cut(s) 116, 999
Tru9I TTAA 2 cut(s) 116, 999
TscAI CASTG 3 cut(s) 730, 922, 977
TseFI GTSAC 2 cut(s) 863, 884
TseI GCWGC 1 cut(s) 567
Tsp45I GTSAC 2 cut(s) 863, 884
TspDTI ATGAA 4 cut(s) 339, 731, 761, 800
TspGWI ACGGA 1 cut(s) 26
TspMI CCCGGG 1 cut(s) 263
TspRI CASTG 3 cut(s) 730, 922, 977
Tth111I GACNNNGTC 1 cut(s) 737
VpaK11BI GGWCC 1 cut(s) 554
XagI CCTNNNNNAGG 1 cut(s) 642
XapI RAATTY 1 cut(s) 391
XmaI CCCGGG 1 cut(s) 263
XmaJI CCTAGG 1 cut(s) 814
XmiI GTMKAC 1 cut(s) 701
XspI CTAG 8 cut(s) 176, 240, 468, 815, 930, 1004, 1008, 1162
ZrmI AGTACT 1 cut(s) 184
Zsp2I ATGCAT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.