Rorug04G0105100

histone-lysine N-methyltransferase, H3 lysine-9 specific

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001110
Physical Location & Seq
Forward (+)
16305120 .. 16305890
771 bp
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UTR
Exon/CDS
Intron
Rorug04G0105100.1

Sequence Viewer

Length: 609 bp
ATGGCGGAAGTCAAGCTCTTTAAGACATGGTCAAGCTCTTTTGCTTTGAGGATAGTTTGGGCACTGAAGCTCAAAGATGTCCCATATGATACCATATTTGAAGATCTCTCAAACAAAAGCCCTTTACTTCTTCAATACAACCTCATTCATAAGAAGGTTCCAGTGCTTGTGCACAATGGAAACTCAATTGCTGAATCTTTGGTGATCCTTGAATACATTGAGGAAACATGGAAACAAAATCCTTTGCTGCCTGAAGATCTTCACGATAAAGCCGCTGCACGATTTTGGGCCAAATTTGGTGATGACCAGATTTTTCCACCTATTGTGGACGCATTATGCAGTGAAGGGAAAGAGCAAGAGGAAGCTATTGTGAAAGCAAGGGAGAACTTGAAGTACTTGCAAGAAGAGCTAAAGGGAAAGAAATTCTTTGCGGGAAAGCAAATTGGATTTGCAGATATCGCGCTCAGATGGCTTGCACAACATGAGAACGTGTTTGAAGAGGTAGCTAGCATGAAGCTGATAGCAGAAGATGAGTTCCCATTGTTATCCCAATGGCAAAAGATCGACTTTATCAGATGCTCCTATAATCAAAGAGAATTGGGCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SAD_SRA SET

Protein Analysis

202

Amino Acids

23.39

Weight (kDa)

5.35

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 3 - 75 1.5e-18 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 6 - 78 1.4e-11 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 12 - 76 3.1e-13 Glutathione S-transferase, N-terminal domain
GST_C PF00043 115 - 185 8.6e-10 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 461
AciI CCGC 3 cut(s) 5, 273, 431
AclWI GGATC 1 cut(s) 199
AcsI RAATTY 2 cut(s) 293, 422
AcuI CTGAAG 2 cut(s) 86, 273
AfaI GTAC 1 cut(s) 395
AflIII ACRYGT 1 cut(s) 489
AgsI TTSAA 5 cut(s) 101, 134, 212, 391, 497
AluBI AGCT 7 cut(s) 16, 36, 70, 365, 409, 506, 517
AluI AGCT 7 cut(s) 16, 36, 70, 365, 409, 506, 517
Alw21I GWGCWC 1 cut(s) 174
Alw44I GTGCAC 1 cut(s) 170
AlwI GGATC 1 cut(s) 199
AoxI GGCC 1 cut(s) 288
ApaLI GTGCAC 1 cut(s) 170
ApeKI GCWGC 2 cut(s) 247, 275
ApoI RAATTY 2 cut(s) 293, 422
Asp700I GAANNNNTTC 1 cut(s) 258
AspLEI GCGC 1 cut(s) 463
AspS9I GGNCC 1 cut(s) 288
AsuHPI GGTGA 2 cut(s) 214, 311
AsuNHI GCTAGC 1 cut(s) 506
BaeGI GKGCMC 2 cut(s) 64, 174
BanII GRGCYC 1 cut(s) 605
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 2 cut(s) 234, 262
BccI CCATC 1 cut(s) 462
BfaI CTAG 2 cut(s) 507, 607
BglII AGATCT 2 cut(s) 103, 256
BisI GCNGC 3 cut(s) 248, 273, 276
BlsI GCNGC 3 cut(s) 249, 274, 277
BmcAI AGTACT 1 cut(s) 395
BmgT120I GGNCC 1 cut(s) 288
BmiI GGNNCC 2 cut(s) 159, 604
BmsI GCATC 1 cut(s) 566
BmtI GCTAGC 1 cut(s) 510
Bse1I ACTGG 1 cut(s) 161
BseMII CTCAG 1 cut(s) 478
BseNI ACTGG 1 cut(s) 161
BseSI GKGCMC 2 cut(s) 64, 174
BseXI GCAGC 2 cut(s) 234, 262
BsgI GTGCAG 1 cut(s) 261
Bsh1236I CGCG 1 cut(s) 461
BshFI GGCC 1 cut(s) 290
BsiHKAI GWGCWC 1 cut(s) 174
BslFI GGGAC 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 65
BsnI GGCC 1 cut(s) 290
Bsp1286I GDGCHC 3 cut(s) 64, 174, 605
Bsp143I GATC 4 cut(s) 103, 204, 256, 561
BspACI CCGC 3 cut(s) 5, 273, 431
BspANI GGCC 1 cut(s) 290
BspCNI CTCAG 1 cut(s) 477
BspFNI CGCG 1 cut(s) 461
BspLI GGNNCC 2 cut(s) 159, 604
BspOI GCTAGC 1 cut(s) 510
BspPI GGATC 1 cut(s) 199
BspQI GCTCTTC 1 cut(s) 399
BsrI ACTGG 1 cut(s) 161
BssMI GATC 4 cut(s) 103, 204, 256, 561
Bst6I CTCTTC 2 cut(s) 399, 492
BstC8I GCNNGC 2 cut(s) 474, 508
BstDEI CTNAG 1 cut(s) 464
BstFNI CGCG 1 cut(s) 461
BstHHI GCGC 1 cut(s) 463
BstKTI GATC 4 cut(s) 106, 207, 259, 564
BstMBI GATC 4 cut(s) 103, 204, 256, 561
BstMWI GCNNNNNNNGC 3 cut(s) 406, 458, 469
BstSLI GKGCMC 2 cut(s) 64, 174
BstUI CGCG 1 cut(s) 461
BstV1I GCAGC 2 cut(s) 234, 262
BstX2I RGATCY 2 cut(s) 103, 256
BstYI RGATCY 2 cut(s) 103, 256
BsuRI GGCC 1 cut(s) 290
BtsI GCAGTG 1 cut(s) 346
BtsIMutI CAGTG 3 cut(s) 62, 168, 346
Cac8I GCNNGC 2 cut(s) 474, 508
CfoI GCGC 1 cut(s) 463
Cfr13I GGNCC 1 cut(s) 288
CseI GACGC 1 cut(s) 338
Csp6I GTAC 1 cut(s) 394
CviAII CATG 4 cut(s) 27, 228, 482, 511
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 1 cut(s) 464
DpnI GATC 4 cut(s) 105, 206, 258, 563
DpnII GATC 4 cut(s) 103, 204, 256, 561
Eam1104I CTCTTC 2 cut(s) 399, 492
EarI CTCTTC 2 cut(s) 399, 492
EciI GGCGGA 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 605
Eco32I GATATC 1 cut(s) 457
Eco57I CTGAAG 2 cut(s) 86, 273
EcoRV GATATC 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 605
FaeI CATG 4 cut(s) 30, 231, 485, 514
FalI AAGNNNNNCTT 4 cut(s) 410, 442, 551, 583
FaqI GGGAC 1 cut(s) 65
FatI CATG 4 cut(s) 26, 227, 481, 510
FauI CCCGC 1 cut(s) 424
FauNDI CATATG 1 cut(s) 85
Fnu4HI GCNGC 3 cut(s) 248, 273, 276
FriOI GRGCYC 1 cut(s) 605
Fsp4HI GCNGC 3 cut(s) 248, 273, 276
FspBI CTAG 2 cut(s) 507, 607
GlaI GCGC 1 cut(s) 462
GluI GCNGC 3 cut(s) 248, 273, 276
HaeIII GGCC 1 cut(s) 290
HgaI GACGC 1 cut(s) 338
HhaI GCGC 1 cut(s) 463
Hin1II CATG 4 cut(s) 30, 231, 485, 514
Hin6I GCGC 1 cut(s) 461
HinP1I GCGC 1 cut(s) 461
HinfI GANTC 1 cut(s) 194
HphI GGTGA 2 cut(s) 214, 311
Hpy166II GTNNAC 2 cut(s) 172, 328
Hpy188I TCNGA 2 cut(s) 467, 575
Hpy188III TCNNGA 1 cut(s) 263
Hpy8I GTNNAC 2 cut(s) 172, 328
HpyAV CCTTC 2 cut(s) 148, 338
HpyCH4IV ACGT 1 cut(s) 489
HpyCH4V TGCA 6 cut(s) 172, 278, 339, 400, 452, 476
HpyF10VI GCNNNNNNNGC 3 cut(s) 406, 458, 469
HpyF3I CTNAG 1 cut(s) 464
HpySE526I ACGT 1 cut(s) 489
Hsp92II CATG 4 cut(s) 30, 231, 485, 514
HspAI GCGC 1 cut(s) 461
Kzo9I GATC 4 cut(s) 103, 204, 256, 561
LguI GCTCTTC 1 cut(s) 399
LmnI GCTCC 2 cut(s) 584, 608
LpnPI CCDG 3 cut(s) 174, 264, 320
Lsp1109I GCAGC 2 cut(s) 234, 262
LweI GCATC 1 cut(s) 566
MaeI CTAG 2 cut(s) 507, 607
MaeII ACGT 1 cut(s) 489
MalI GATC 4 cut(s) 105, 206, 258, 563
MboI GATC 4 cut(s) 103, 204, 256, 561
MboII GAAGA 7 cut(s) 113, 122, 251, 266, 416, 509, 539
MfeI CAATTG 1 cut(s) 186
MflI RGATCY 2 cut(s) 103, 256
MhlI GDGCHC 3 cut(s) 64, 174, 605
MluCI AATT 5 cut(s) 186, 293, 422, 441, 596
MnlI CCTC 5 cut(s) 42, 152, 214, 352, 493
MroXI GAANNNNTTC 1 cut(s) 258
MseI TTAA 1 cut(s) 21
MspA1I CMGCKG 1 cut(s) 275
MunI CAATTG 1 cut(s) 186
MvnI CGCG 1 cut(s) 461
MwoI GCNNNNNNNGC 3 cut(s) 406, 458, 469
NdeI CATATG 1 cut(s) 85
NdeII GATC 4 cut(s) 103, 204, 256, 561
NheI GCTAGC 1 cut(s) 506
NlaIII CATG 4 cut(s) 30, 231, 485, 514
NlaIV GGNNCC 2 cut(s) 159, 604
PciSI GCTCTTC 1 cut(s) 399
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 1 cut(s) 194
PflFI GACNNNGTC 1 cut(s) 28
PkrI GCNGC 3 cut(s) 249, 274, 277
PspN4I GGNNCC 2 cut(s) 159, 604
PspPI GGNCC 1 cut(s) 288
PsrI GAACNNNNNNTAC 2 cut(s) 377, 409
PsuI RGATCY 2 cut(s) 103, 256
PsyI GACNNNGTC 1 cut(s) 28
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
SapI GCTCTTC 1 cut(s) 399
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 3 cut(s) 248, 273, 276
Sau3AI GATC 4 cut(s) 103, 204, 256, 561
Sau96I GGNCC 1 cut(s) 288
ScaI AGTACT 1 cut(s) 395
SduI GDGCHC 3 cut(s) 64, 174, 605
SfaNI GCATC 1 cut(s) 566
Sse9I AATT 5 cut(s) 186, 293, 422, 441, 596
SsiI CCGC 3 cut(s) 5, 273, 431
SspMI CTAG 2 cut(s) 507, 607
TaiI ACGT 1 cut(s) 492
TaqI TCGA 1 cut(s) 564
TasI AATT 5 cut(s) 186, 293, 422, 441, 596
TatI WGTACW 1 cut(s) 393
TauI GCSGC 1 cut(s) 275
TfiI GAWTC 1 cut(s) 194
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TscAI CASTG 3 cut(s) 69, 168, 346
TseI GCWGC 2 cut(s) 247, 275
TspDTI ATGAA 2 cut(s) 137, 527
TspRI CASTG 3 cut(s) 69, 168, 346
Tth111I GACNNNGTC 1 cut(s) 28
VneI GTGCAC 1 cut(s) 170
XapI RAATTY 2 cut(s) 293, 422
XmnI GAANNNNTTC 1 cut(s) 258
XspI CTAG 2 cut(s) 507, 607
ZrmI AGTACT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.