Rmu_sc0010364.1_g000006

histone-lysine N-methyltransferase, H3 lysine-9 specific

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010364.1
OG0001110
Physical Location & Seq
Forward (+)
31143 .. 32375
1233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010364.1_g000006.1.cds

Sequence Viewer

Length: 1233 bp
atgggcgccttaaagggctccaccgatgagcctagggttagccccggccggcaaaaccgtcggaaacggatgtcatcaacggatgcgaagaaagacgggcgaaagaaggccaaggttgatggggatacagagactttggaggtaaacaatttagggattagttggtttgctccatttactgttttaagcacgagctctattagcttttttagaagcttgagagaaaatcttagagttgacaatctagtcagtactgatcagttccattttactcgtaagagtcaacgggctataggtaaagagtcttctagggttgttgagaatgatttacctgggttggtctgcaacgacctttgtaatggaaaagaaaacatatgcatccccgttacaaatgaaattgatgtccctcctgtagctcccgaaaagtttgagtatctcagctccatcgaagtagcaaaaaatttggtcattccggagagttctgagggatgtaattgcaaagggaagtgtactaatacgaggacttgttcgtgtgctagcctcaatggatctgactttccttacgttcggaaagccggtggcggcagattgtttgagcctaaggctgttgttttcgagtgtggtccgaactgtggctgcgggcccaagtgtgtgaaccgcacttctcagcagggattgaagtacaggcttgaggtttataagacacctgacaaaggctgggctgttaggtcttgggattttattccctctggtggttatgtttgcgagtatactggtgttctgatgaagaccagtgaactggacaacgtctccatgaatgactacttttttgaaattgattgtgagcatacaatgaatgggattggagtgagggagaagcgcctaggtgaagtgcccatttcaaatggtcttggtgctggcgaagatggaggtgacaagttattagaagaaagtgaccccgactattgtatagatgctggatccagtgggaatgttgctaggtttatcaaccatagttgcgagccgaatctgtttgtgcagtgtgtcttgagttctcaccatgatgttaaactagctagaattgtgttgtttgcttccgatgacatacctcctatgcaagagctgacttatgactatggctatgagcttgatagtgtcgttggtgctgatggaaaagtcaaggagttggcttgtcattgtggtacagcggactgtcggaagcgtttgtactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SAD_SRA SET

Protein Analysis

410

Amino Acids

45.11

Weight (kDa)

5.7

Isoelectric Point (pI)

33.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 699
AasI GACNNNNNNGTC 1 cut(s) 245
AccB1I GGYRCC 1 cut(s) 5
AccI GTMKAC 1 cut(s) 770
AccIII TCCGGA 1 cut(s) 472
AciI CCGC 4 cut(s) 582, 639, 658, 1208
AclWI GGATC 3 cut(s) 556, 975, 988
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 1 cut(s) 460
AcyI GRCGYC 1 cut(s) 6
AfaI GTAC 5 cut(s) 253, 511, 683, 1204, 1229
AfiI CCNNNNNNNGG 3 cut(s) 632, 713, 752
AgsI TTSAA 3 cut(s) 679, 833, 903
AjnI CCWGG 1 cut(s) 331
AluBI AGCT 8 cut(s) 195, 204, 216, 416, 441, 1076, 1123, 1147
AluI AGCT 8 cut(s) 195, 204, 216, 416, 441, 1076, 1123, 1147
Alw21I GWGCWC 1 cut(s) 197
Alw26I GTCTC 2 cut(s) 125, 814
AlwI GGATC 3 cut(s) 556, 975, 988
Aor13HI TCCGGA 1 cut(s) 472
AoxI GGCC 3 cut(s) 46, 108, 641
ApaI GGGCCC 1 cut(s) 645
ApeKI GCWGC 1 cut(s) 636
ApoI RAATTY 1 cut(s) 460
ArsI GACNNNNNNTTYG 2 cut(s) 812, 844
AspA2I CCTAGG 2 cut(s) 32, 883
AspLEI GCGC 2 cut(s) 8, 882
AspS9I GGNCC 3 cut(s) 623, 641, 642
AsuC2I CCSGG 1 cut(s) 45
AsuHPI GGTGA 3 cut(s) 899, 944, 1049
AsuNHI GCTAGC 1 cut(s) 536
AvaII GGWCC 1 cut(s) 623
AvrII CCTAGG 2 cut(s) 32, 883
AxyI CCTNAGG 1 cut(s) 600
BaeGI GKGCMC 2 cut(s) 645, 897
BamHI GGATCC 1 cut(s) 980
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 3 cut(s) 20, 197, 645
BarI GAAGNNNNNNTAC 3 cut(s) 289, 321, 1211
BauI CACGAG 1 cut(s) 190
BbsI GAAGAC 2 cut(s) 297, 794
Bbv12I GWGCWC 1 cut(s) 197
BbvI GCAGC 1 cut(s) 623
BccI CCATC 4 cut(s) 113, 452, 920, 1163
BcgI CGANNNNNNTGC 2 cut(s) 41, 75
BciT130I CCWGG 1 cut(s) 333
BciVI GTATCC 1 cut(s) 118
BclI TGATCA 1 cut(s) 256
BcnI CCSGG 1 cut(s) 45
BcoDI GTCTC 2 cut(s) 125, 814
BfaI CTAG 9 cut(s) 33, 245, 309, 537, 884, 999, 1073, 1077, 1231
BfmI CTRYAG 2 cut(s) 291, 411
BfoI RGCGCY 2 cut(s) 9, 883
BfuI GTATCC 1 cut(s) 118
BisI GCNGC 2 cut(s) 583, 637
BlnI CCTAGG 2 cut(s) 32, 883
BlsI GCNGC 2 cut(s) 584, 638
BmcAI AGTACT 1 cut(s) 253
Bme1390I CCNGG 2 cut(s) 45, 333
Bme18I GGWCC 1 cut(s) 623
BmgT120I GGNCC 3 cut(s) 623, 641, 642
BmiI GGNNCC 4 cut(s) 7, 19, 643, 982
BmrFI CCNGG 2 cut(s) 45, 333
BmsI GCATC 3 cut(s) 73, 387, 964
BmtI GCTAGC 1 cut(s) 540
BpiI GAAGAC 2 cut(s) 297, 794
BpuEI CTTGAG 3 cut(s) 238, 710, 1069
BpuMI CCSGG 1 cut(s) 45
BsaHI GRCGYC 1 cut(s) 6
BsaJI CCNNGG 5 cut(s) 32, 43, 111, 332, 883
BsaWI WCCGGW 1 cut(s) 472
BsaXI ACNNNNNCTCC 2 cut(s) 794, 824
Bsc4I CCNNNNNNNGG 3 cut(s) 632, 713, 752
Bse118I RCCGGY 2 cut(s) 48, 575
Bse1I ACTGG 4 cut(s) 778, 792, 804, 984
Bse21I CCTNAGG 1 cut(s) 600
BseAI TCCGGA 1 cut(s) 472
BseBI CCWGG 1 cut(s) 333
BseDI CCNNGG 5 cut(s) 32, 43, 111, 332, 883
BseGI GGATG 4 cut(s) 75, 88, 378, 494
BseLI CCNNNNNNNGG 3 cut(s) 632, 713, 752
BseMII CTCAG 3 cut(s) 451, 474, 680
BseNI ACTGG 4 cut(s) 778, 792, 804, 984
BseSI GKGCMC 2 cut(s) 645, 897
BseX3I CGGCCG 1 cut(s) 46
BseXI GCAGC 1 cut(s) 623
BseYI CCCAGC 1 cut(s) 717
BsgI GTGCAG 1 cut(s) 1058
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 3 cut(s) 48, 110, 643
BshNI GGYRCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 49
BsiHKAI GWGCWC 1 cut(s) 197
BsiSI CCGG 4 cut(s) 45, 49, 473, 576
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 3 cut(s) 632, 713, 752
BsmAI GTCTC 2 cut(s) 125, 814
BsmBI CGTCTC 1 cut(s) 814
BsmFI GGGAC 1 cut(s) 389
BsnI GGCC 3 cut(s) 48, 110, 643
Bsp120I GGGCCC 1 cut(s) 641
Bsp1286I GDGCHC 4 cut(s) 20, 197, 645, 897
Bsp13I TCCGGA 1 cut(s) 472
Bsp143I GATC 3 cut(s) 256, 548, 980
BspACI CCGC 4 cut(s) 582, 639, 658, 1208
BspANI GGCC 3 cut(s) 48, 110, 643
BspCNI CTCAG 3 cut(s) 450, 475, 679
BspEI TCCGGA 1 cut(s) 472
BspLI GGNNCC 4 cut(s) 7, 19, 643, 982
BspOI GCTAGC 1 cut(s) 540
BspPI GGATC 3 cut(s) 556, 975, 988
BspT107I GGYRCC 1 cut(s) 5
BsrFI RCCGGY 2 cut(s) 48, 575
BsrI ACTGG 4 cut(s) 778, 792, 804, 984
BssAI RCCGGY 2 cut(s) 48, 575
BssECI CCNNGG 5 cut(s) 32, 43, 111, 332, 883
BssMI GATC 3 cut(s) 256, 548, 980
BssNAI GTATAC 1 cut(s) 771
BssNI GRCGYC 1 cut(s) 6
BssSI CACGAG 1 cut(s) 190
BssT1I CCWWGG 3 cut(s) 32, 111, 883
Bst1107I GTATAC 1 cut(s) 771
Bst2BI CACGAG 1 cut(s) 190
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 4 cut(s) 59, 181, 632, 1214
BstACI GRCGYC 1 cut(s) 6
BstC8I GCNNGC 5 cut(s) 50, 538, 641, 919, 1022
BstDEI CTNAG 5 cut(s) 230, 437, 483, 600, 666
BstENI CCTNNNNNAGG 1 cut(s) 711
BstF5I GGATG 4 cut(s) 75, 88, 378, 494
BstH2I RGCGCY 2 cut(s) 9, 883
BstHHI GCGC 2 cut(s) 8, 882
BstKTI GATC 3 cut(s) 259, 551, 983
BstMAI GTCTC 2 cut(s) 125, 814
BstMBI GATC 3 cut(s) 256, 548, 980
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 1 cut(s) 201
BstNI CCWGG 1 cut(s) 333
BstSCI CCNGG 2 cut(s) 43, 331
BstSFI CTRYAG 2 cut(s) 291, 411
BstSLI GKGCMC 2 cut(s) 645, 897
BstV1I GCAGC 1 cut(s) 623
BstV2I GAAGAC 2 cut(s) 297, 794
BstX2I RGATCY 2 cut(s) 548, 980
BstXI CCANNNNNNTGG 1 cut(s) 799
BstYI RGATCY 2 cut(s) 548, 980
BstZ17I GTATAC 1 cut(s) 771
BstZI CGGCCG 1 cut(s) 46
Bsu36I CCTNAGG 1 cut(s) 600
BsuI GTATCC 1 cut(s) 118
BsuRI GGCC 3 cut(s) 48, 110, 643
BtsCI GGATG 4 cut(s) 75, 88, 378, 494
BtsI GCAGTG 1 cut(s) 1046
BtsIMutI CAGTG 3 cut(s) 799, 991, 1046
Cac8I GCNNGC 5 cut(s) 50, 538, 641, 919, 1022
CfoI GCGC 2 cut(s) 8, 882
Cfr10I RCCGGY 2 cut(s) 48, 575
Cfr13I GGNCC 3 cut(s) 623, 641, 642
Csp6I GTAC 5 cut(s) 252, 510, 682, 1203, 1228
CviAII CATG 2 cut(s) 814, 1061
CviQI GTAC 5 cut(s) 252, 510, 682, 1203, 1228
DdeI CTNAG 5 cut(s) 230, 437, 483, 600, 666
DinI GGCGCC 1 cut(s) 7
DpnI GATC 3 cut(s) 258, 550, 982
DpnII GATC 3 cut(s) 256, 548, 980
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
EaeI YGGCCR 1 cut(s) 46
EagI CGGCCG 1 cut(s) 46
Ecl136II GAGCTC 1 cut(s) 195
EclXI CGGCCG 1 cut(s) 46
Eco130I CCWWGG 3 cut(s) 32, 111, 883
Eco24I GRGCYC 3 cut(s) 20, 197, 645
Eco47I GGWCC 1 cut(s) 623
Eco52I CGGCCG 1 cut(s) 46
Eco53kI GAGCTC 1 cut(s) 195
Eco81I CCTNAGG 1 cut(s) 600
EcoICRI GAGCTC 1 cut(s) 195
EcoNI CCTNNNNNAGG 1 cut(s) 711
EcoRII CCWGG 1 cut(s) 331
EcoT14I CCWWGG 3 cut(s) 32, 111, 883
EcoT22I ATGCAT 1 cut(s) 380
EcoT38I GRGCYC 3 cut(s) 20, 197, 645
EgeI GGCGCC 1 cut(s) 7
EheI GGCGCC 1 cut(s) 7
ErhI CCWWGG 3 cut(s) 32, 111, 883
Esp3I CGTCTC 1 cut(s) 814
FaeI CATG 2 cut(s) 817, 1064
FaqI GGGAC 1 cut(s) 389
FatI CATG 2 cut(s) 813, 1060
FauI CCCGC 1 cut(s) 632
FauNDI CATATG 1 cut(s) 374
FbaI TGATCA 1 cut(s) 256
FblI GTMKAC 1 cut(s) 770
Fnu4HI GCNGC 2 cut(s) 583, 637
FokI GGATG 4 cut(s) 82, 95, 365, 501
FriOI GRGCYC 3 cut(s) 20, 197, 645
Fsp4HI GCNGC 2 cut(s) 583, 637
FspBI CTAG 9 cut(s) 33, 245, 309, 537, 884, 999, 1073, 1077, 1231
GlaI GCGC 2 cut(s) 7, 881
GluI GCNGC 2 cut(s) 583, 637
GsaI CCCAGC 1 cut(s) 721
HaeII RGCGCY 2 cut(s) 9, 883
HaeIII GGCC 3 cut(s) 48, 110, 643
HapII CCGG 4 cut(s) 45, 49, 473, 576
HhaI GCGC 2 cut(s) 8, 882
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 2 cut(s) 817, 1064
Hin6I GCGC 2 cut(s) 6, 880
HinP1I GCGC 2 cut(s) 6, 880
HincII GTYRAC 2 cut(s) 238, 284
HindII GTYRAC 2 cut(s) 238, 284
HindIII AAGCTT 1 cut(s) 214
HinfI GANTC 3 cut(s) 280, 302, 1027
HpaII CCGG 4 cut(s) 45, 49, 473, 576
HphI GGTGA 3 cut(s) 899, 944, 1049
Hpy166II GTNNAC 7 cut(s) 145, 238, 284, 510, 655, 771, 797
Hpy188I TCNGA 8 cut(s) 63, 484, 553, 570, 627, 783, 1099, 1218
Hpy188III TCNNGA 3 cut(s) 419, 473, 1048
Hpy8I GTNNAC 7 cut(s) 145, 238, 284, 510, 655, 771, 797
Hpy99I CGWCG 1 cut(s) 63
HpyAV CCTTC 1 cut(s) 100
HpyCH4III ACNGT 4 cut(s) 59, 181, 632, 1214
HpyCH4IV ACGT 2 cut(s) 564, 807
HpyCH4V TGCA 5 cut(s) 345, 378, 498, 1039, 1117
HpyF10VI GCNNNNNNNGC 1 cut(s) 201
HpyF3I CTNAG 5 cut(s) 230, 437, 483, 600, 666
HpySE526I ACGT 2 cut(s) 564, 807
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 2 cut(s) 817, 1064
HspAI GCGC 2 cut(s) 6, 880
KasI GGCGCC 1 cut(s) 5
Kpn2I TCCGGA 1 cut(s) 472
KroI GCCGGC 1 cut(s) 48
KroNI GCCGGC 1 cut(s) 50
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 3 cut(s) 256, 548, 980
LmnI GCTCC 4 cut(s) 23, 175, 421, 446
Lsp1109I GCAGC 1 cut(s) 623
LweI GCATC 3 cut(s) 73, 387, 964
MaeI CTAG 9 cut(s) 33, 245, 309, 537, 884, 999, 1073, 1077, 1231
MaeII ACGT 2 cut(s) 564, 807
MaeIII GTNAC 3 cut(s) 385, 932, 953
MalI GATC 3 cut(s) 258, 550, 982
MboI GATC 3 cut(s) 256, 548, 980
MboII GAAGA 5 cut(s) 100, 297, 799, 935, 959
MflI RGATCY 2 cut(s) 548, 980
MhlI GDGCHC 4 cut(s) 20, 197, 645, 897
MluCI AATT 6 cut(s) 148, 396, 460, 493, 834, 1080
Mly113I GGCGCC 1 cut(s) 6
MlyI GAGTC 2 cut(s) 289, 311
MmeI TCCRAC 2 cut(s) 41, 1196
Mph1103I ATGCAT 1 cut(s) 380
MroI TCCGGA 1 cut(s) 472
MroNI GCCGGC 1 cut(s) 48
MseI TTAA 3 cut(s) 11, 185, 1068
MslI CAYNNNNRTG 1 cut(s) 1062
MspA1I CMGCKG 1 cut(s) 1208
MspI CCGG 4 cut(s) 45, 49, 473, 576
MspR9I CCNGG 2 cut(s) 45, 333
MvaI CCWGG 1 cut(s) 333
MwoI GCNNNNNNNGC 1 cut(s) 201
NaeI GCCGGC 1 cut(s) 50
NarI GGCGCC 1 cut(s) 6
NciI CCSGG 1 cut(s) 45
NdeI CATATG 1 cut(s) 374
NdeII GATC 3 cut(s) 256, 548, 980
NgoMIV GCCGGC 1 cut(s) 48
NheI GCTAGC 1 cut(s) 536
NlaIII CATG 2 cut(s) 817, 1064
NlaIV GGNNCC 4 cut(s) 7, 19, 643, 982
NmuCI GTSAC 2 cut(s) 932, 953
NsiI ATGCAT 1 cut(s) 380
PdiI GCCGGC 1 cut(s) 50
PfeI GAWTC 1 cut(s) 1027
PflFI GACNNNGTC 1 cut(s) 806
PkrI GCNGC 2 cut(s) 584, 638
PleI GAGTC 2 cut(s) 288, 310
PluTI GGCGCC 1 cut(s) 9
PpsI GAGTC 2 cut(s) 288, 310
PsiI TTATAA 1 cut(s) 699
Psp124BI GAGCTC 1 cut(s) 197
Psp6I CCWGG 1 cut(s) 331
PspFI CCCAGC 1 cut(s) 717
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 4 cut(s) 7, 19, 643, 982
PspOMI GGGCCC 1 cut(s) 641
PspPI GGNCC 3 cut(s) 623, 641, 642
PsuI RGATCY 2 cut(s) 548, 980
PsyI GACNNNGTC 1 cut(s) 806
RsaI GTAC 5 cut(s) 253, 511, 683, 1204, 1229
RsaNI GTAC 5 cut(s) 252, 510, 682, 1203, 1228
RseI CAYNNNNRTG 1 cut(s) 1062
SacI GAGCTC 1 cut(s) 197
SaqAI TTAA 3 cut(s) 11, 185, 1068
SatI GCNGC 2 cut(s) 583, 637
Sau3AI GATC 3 cut(s) 256, 548, 980
Sau96I GGNCC 3 cut(s) 623, 641, 642
ScaI AGTACT 1 cut(s) 253
SchI GAGTC 2 cut(s) 289, 311
ScrFI CCNGG 2 cut(s) 45, 333
SduI GDGCHC 4 cut(s) 20, 197, 645, 897
SfaNI GCATC 3 cut(s) 73, 387, 964
SfcI CTRYAG 2 cut(s) 291, 411
SfoI GGCGCC 1 cut(s) 7
SinI GGWCC 1 cut(s) 623
SmiMI CAYNNNNRTG 1 cut(s) 1062
SmlI CTYRAG 3 cut(s) 217, 689, 1048
SmoI CTYRAG 3 cut(s) 217, 689, 1048
Sse9I AATT 6 cut(s) 148, 396, 460, 493, 834, 1080
SsiI CCGC 4 cut(s) 582, 639, 658, 1208
SspDI GGCGCC 1 cut(s) 5
SspMI CTAG 9 cut(s) 33, 245, 309, 537, 884, 999, 1073, 1077, 1231
SstI GAGCTC 1 cut(s) 197
StyD4I CCNGG 2 cut(s) 43, 331
StyI CCWWGG 3 cut(s) 32, 111, 883
TaaI ACNGT 4 cut(s) 59, 181, 632, 1214
TaiI ACGT 2 cut(s) 567, 810
TaqI TCGA 2 cut(s) 447, 615
TasI AATT 6 cut(s) 148, 396, 460, 493, 834, 1080
TatI WGTACW 4 cut(s) 251, 509, 681, 1227
TauI GCSGC 1 cut(s) 585
TfiI GAWTC 1 cut(s) 1027
Tru1I TTAA 3 cut(s) 11, 185, 1068
Tru9I TTAA 3 cut(s) 11, 185, 1068
TscAI CASTG 3 cut(s) 799, 991, 1046
TseFI GTSAC 2 cut(s) 932, 953
TseI GCWGC 1 cut(s) 636
Tsp45I GTSAC 2 cut(s) 932, 953
TspDTI ATGAA 4 cut(s) 408, 800, 830, 869
TspGWI ACGGA 2 cut(s) 82, 95
TspRI CASTG 3 cut(s) 799, 991, 1046
Tth111I GACNNNGTC 1 cut(s) 806
VpaK11BI GGWCC 1 cut(s) 623
XagI CCTNNNNNAGG 1 cut(s) 711
XapI RAATTY 1 cut(s) 460
XmaJI CCTAGG 2 cut(s) 32, 883
XmiI GTMKAC 1 cut(s) 770
XspI CTAG 9 cut(s) 33, 245, 309, 537, 884, 999, 1073, 1077, 1231
ZrmI AGTACT 1 cut(s) 253
Zsp2I ATGCAT 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.