RLG00000008308

histone-lysine N-methyltransferase, H3 lysine-9 specific

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001110
Physical Location & Seq
Forward (+)
28714221 .. 28715685
1465 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008308

Sequence Viewer

Length: 1233 bp
ATGGGAGCCTTAAAGGGCTCCACCGATGAGCCTAGGGTTAGCCCCAGCCGGCAAAACCGTCGGAAACGGATGTCATCAACGGATGCGAAGAAAGGCGGGCGAAAGAAAGCCAAGGTTGATGGTGATACAGAGGCTTTGGAGGTAAACAATTTAGGGATTAGTTGGTTTGCTCCATTTATTGTTTTAAGCACGAGCTCTATTAGCTTTTTTAGAAGCTTGAGAGAAACTCTTAGAGTTGACAATCTAGTCAGTACTGATCAGTTCCATTTTACTCGTAAGAGTCAACGGGCTATAGGTCAAGAGTCTTCTGGGGTTGTTGAGAATGAGTTACCTGGGTTGGTCTGCAACGACCTTTGTAATGGAAAAGAAAACATATGCATCCCTGTTACCAATGAAGTTGATGTCCCTCCTGTAGCTCCCGATAAGTTTGAGTATCTCAGCTCCATTGAAGTAGCAAGTAATTTGGTCATTCCGGCGAGTGCTGAGGGATGTAATTGCAAAGGGAAGTGTACCAATACGAGGACTTGTTCGTGTGCTAGCCTCAATCGATCTGACTTTCCTTATGTTCGGAAAGCTGGTGGAGGCAGATTGTTTGAGCCTAAGGCTGTTGTTTTCGAGTGTGGTCCGAACTGTGGCTGCGGGCCTAAGTGTGTGAACCGCACTTCTCAGCAGGGATTGAAGTACAGGCTTGAGGTTTATAAGACACCTGACAAAGGCTGGGCTGTTCGGTCTTGGGATTTTATTCCCTCTGGTGGTTATGTTTGCGAGTATACTGGTGTCCTGATGAAGACCAGTGAACTGGACAATGTCTCCGTCAATGATTACTTTTTTGAAATTGATTGTGAGCATACAATGAATGGGATTGGAGGGAGGGAGAAGCGCCTGGGTGAAGTGCCCATTCCAAATGGTCTTGGTGCTGGCGAAGATGGAGGTGACAAGTTATTAGAAGAAAGTGACCCCGACTATTGTATAGATGCTGGATCCAGTGGGAATGTTGCTAGGTTTATCAACCATAGTTGCGAGCCGAATCTGTTTGTGCAGTGTGTCTTGAGTTCTCACCATGATGTTAAACTAGCTAGAATTGTGTTGTTTGCTTCCGATGACATACCTCCTATGCAAGAGCTGACTTATGACTATGGCTATGAGCTTGATAGTGTCGTTGGTGCTGATGGAAAAGTGAAAGAGTTGGCTTGTCGTTGTGGTACAGCGGACTGTCGGAAGCGTTTGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SAD_SRA SET

Protein Analysis

411

Amino Acids

44.88

Weight (kDa)

5.74

Isoelectric Point (pI)

32.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pre-SET PF05033 120 - 220 3.6e-17 Pre-SET motif
SET PF00856 239 - 380 5.7e-20 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 699
AasI GACNNNNNNGTC 1 cut(s) 245
AccI GTMKAC 1 cut(s) 770
AciI CCGC 4 cut(s) 96, 639, 658, 1208
AclWI GGATC 2 cut(s) 975, 988
AfaI GTAC 5 cut(s) 253, 511, 683, 1204, 1229
AfiI CCNNNNNNNGG 4 cut(s) 519, 632, 713, 752
AgsI TTSAA 3 cut(s) 449, 679, 833
AjnI CCWGG 2 cut(s) 331, 882
AluBI AGCT 9 cut(s) 195, 204, 216, 416, 441, 575, 1076, 1123, 1147
AluI AGCT 9 cut(s) 195, 204, 216, 416, 441, 575, 1076, 1123, 1147
Alw21I GWGCWC 1 cut(s) 197
Alw26I GTCTC 1 cut(s) 814
AlwI GGATC 2 cut(s) 975, 988
AoxI GGCC 1 cut(s) 641
ApeKI GCWGC 1 cut(s) 636
AspA2I CCTAGG 1 cut(s) 32
AspLEI GCGC 1 cut(s) 882
AspS9I GGNCC 2 cut(s) 623, 641
AsuHPI GGTGA 4 cut(s) 134, 899, 944, 1049
AsuNHI GCTAGC 1 cut(s) 536
AvaII GGWCC 1 cut(s) 623
AvrII CCTAGG 1 cut(s) 32
AxyI CCTNAGG 1 cut(s) 600
BaeGI GKGCMC 1 cut(s) 897
BamHI GGATCC 1 cut(s) 980
BanII GRGCYC 2 cut(s) 20, 197
BarI GAAGNNNNNNTAC 1 cut(s) 1211
BauI CACGAG 1 cut(s) 190
BbsI GAAGAC 2 cut(s) 297, 794
Bbv12I GWGCWC 1 cut(s) 197
BbvCI CCTCAGC 1 cut(s) 483
BbvI GCAGC 1 cut(s) 623
BccI CCATC 3 cut(s) 113, 920, 1163
BcgI CGANNNNNNTGC 2 cut(s) 41, 75
BciT130I CCWGG 2 cut(s) 333, 884
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 1 cut(s) 814
BfaI CTAG 7 cut(s) 33, 245, 537, 999, 1073, 1077, 1231
BfmI CTRYAG 2 cut(s) 291, 411
BfoI RGCGCY 1 cut(s) 883
BisI GCNGC 1 cut(s) 637
BlnI CCTAGG 1 cut(s) 32
BlsI GCNGC 1 cut(s) 638
BmcAI AGTACT 1 cut(s) 253
Bme1390I CCNGG 2 cut(s) 333, 884
Bme18I GGWCC 1 cut(s) 623
BmgT120I GGNCC 2 cut(s) 623, 641
BmiI GGNNCC 3 cut(s) 7, 19, 982
BmrFI CCNGG 2 cut(s) 333, 884
BmsI GCATC 3 cut(s) 73, 387, 964
BmtI GCTAGC 1 cut(s) 540
BpiI GAAGAC 2 cut(s) 297, 794
BplI GAGNNNNNCTC 2 cut(s) 211, 243
Bpu10I CCTNAGC 1 cut(s) 483
BpuEI CTTGAG 3 cut(s) 238, 710, 1069
Bsa29I ATCGAT 1 cut(s) 547
BsaJI CCNNGG 4 cut(s) 32, 111, 332, 883
BsaXI ACNNNNNCTCC 2 cut(s) 794, 824
Bsc4I CCNNNNNNNGG 4 cut(s) 519, 632, 713, 752
Bse118I RCCGGY 1 cut(s) 48
Bse1I ACTGG 4 cut(s) 778, 792, 804, 984
Bse21I CCTNAGG 1 cut(s) 600
BseBI CCWGG 2 cut(s) 333, 884
BseCI ATCGAT 1 cut(s) 547
BseDI CCNNGG 4 cut(s) 32, 111, 332, 883
BseGI GGATG 4 cut(s) 75, 88, 378, 494
BseLI CCNNNNNNNGG 4 cut(s) 519, 632, 713, 752
BseMII CTCAG 3 cut(s) 451, 474, 680
BseNI ACTGG 4 cut(s) 778, 792, 804, 984
BseSI GKGCMC 1 cut(s) 897
BseXI GCAGC 1 cut(s) 623
BseYI CCCAGC 2 cut(s) 44, 717
BsgI GTGCAG 1 cut(s) 1058
BshFI GGCC 1 cut(s) 643
BshVI ATCGAT 1 cut(s) 547
BsiHKAI GWGCWC 1 cut(s) 197
BsiSI CCGG 2 cut(s) 49, 473
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 4 cut(s) 519, 632, 713, 752
BsmAI GTCTC 1 cut(s) 814
BsmFI GGGAC 1 cut(s) 389
BsnI GGCC 1 cut(s) 643
Bsp1286I GDGCHC 3 cut(s) 20, 197, 897
Bsp143I GATC 3 cut(s) 256, 548, 980
BspACI CCGC 4 cut(s) 96, 639, 658, 1208
BspANI GGCC 1 cut(s) 643
BspCNI CTCAG 3 cut(s) 450, 475, 679
BspDI ATCGAT 1 cut(s) 547
BspLI GGNNCC 3 cut(s) 7, 19, 982
BspOI GCTAGC 1 cut(s) 540
BspPI GGATC 2 cut(s) 975, 988
BsrFI RCCGGY 1 cut(s) 48
BsrI ACTGG 4 cut(s) 778, 792, 804, 984
BssAI RCCGGY 1 cut(s) 48
BssECI CCNNGG 4 cut(s) 32, 111, 332, 883
BssMI GATC 3 cut(s) 256, 548, 980
BssNAI GTATAC 1 cut(s) 771
BssSI CACGAG 1 cut(s) 190
BssT1I CCWWGG 2 cut(s) 32, 111
Bst1107I GTATAC 1 cut(s) 771
Bst2BI CACGAG 1 cut(s) 190
Bst2UI CCWGG 2 cut(s) 333, 884
Bst4CI ACNGT 3 cut(s) 59, 632, 1214
BstC8I GCNNGC 6 cut(s) 50, 98, 538, 641, 919, 1022
BstDEI CTNAG 6 cut(s) 230, 437, 483, 600, 645, 666
BstENI CCTNNNNNAGG 1 cut(s) 711
BstF5I GGATG 4 cut(s) 75, 88, 378, 494
BstH2I RGCGCY 1 cut(s) 883
BstHHI GCGC 1 cut(s) 882
BstKTI GATC 3 cut(s) 259, 551, 983
BstMAI GTCTC 1 cut(s) 814
BstMBI GATC 3 cut(s) 256, 548, 980
BstMWI GCNNNNNNNGC 1 cut(s) 201
BstNI CCWGG 2 cut(s) 333, 884
BstSCI CCNGG 2 cut(s) 331, 882
BstSFI CTRYAG 2 cut(s) 291, 411
BstSLI GKGCMC 1 cut(s) 897
BstV1I GCAGC 1 cut(s) 623
BstV2I GAAGAC 2 cut(s) 297, 794
BstX2I RGATCY 1 cut(s) 980
BstXI CCANNNNNNTGG 1 cut(s) 799
BstYI RGATCY 1 cut(s) 980
BstZ17I GTATAC 1 cut(s) 771
Bsu15I ATCGAT 1 cut(s) 547
Bsu36I CCTNAGG 1 cut(s) 600
BsuRI GGCC 1 cut(s) 643
BsuTUI ATCGAT 1 cut(s) 547
BtsCI GGATG 4 cut(s) 75, 88, 378, 494
BtsI GCAGTG 1 cut(s) 1046
BtsIMutI CAGTG 3 cut(s) 799, 991, 1046
Cac8I GCNNGC 6 cut(s) 50, 98, 538, 641, 919, 1022
CfoI GCGC 1 cut(s) 882
Cfr10I RCCGGY 1 cut(s) 48
Cfr13I GGNCC 2 cut(s) 623, 641
ClaI ATCGAT 1 cut(s) 547
Csp6I GTAC 5 cut(s) 252, 510, 682, 1203, 1228
CviAII CATG 1 cut(s) 1061
CviQI GTAC 5 cut(s) 252, 510, 682, 1203, 1228
DdeI CTNAG 6 cut(s) 230, 437, 483, 600, 645, 666
DpnI GATC 3 cut(s) 258, 550, 982
DpnII GATC 3 cut(s) 256, 548, 980
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
Ecl136II GAGCTC 1 cut(s) 195
Eco130I CCWWGG 2 cut(s) 32, 111
Eco24I GRGCYC 2 cut(s) 20, 197
Eco47I GGWCC 1 cut(s) 623
Eco53kI GAGCTC 1 cut(s) 195
Eco81I CCTNAGG 1 cut(s) 600
EcoICRI GAGCTC 1 cut(s) 195
EcoNI CCTNNNNNAGG 1 cut(s) 711
EcoRII CCWGG 2 cut(s) 331, 882
EcoT14I CCWWGG 2 cut(s) 32, 111
EcoT22I ATGCAT 1 cut(s) 380
EcoT38I GRGCYC 2 cut(s) 20, 197
ErhI CCWWGG 2 cut(s) 32, 111
FaeI CATG 1 cut(s) 1064
FaqI GGGAC 1 cut(s) 389
FatI CATG 1 cut(s) 1060
FauI CCCGC 2 cut(s) 89, 632
FauNDI CATATG 1 cut(s) 374
FbaI TGATCA 1 cut(s) 256
FblI GTMKAC 1 cut(s) 770
Fnu4HI GCNGC 1 cut(s) 637
FokI GGATG 4 cut(s) 82, 95, 365, 501
FriOI GRGCYC 2 cut(s) 20, 197
Fsp4HI GCNGC 1 cut(s) 637
FspBI CTAG 7 cut(s) 33, 245, 537, 999, 1073, 1077, 1231
GlaI GCGC 1 cut(s) 881
GluI GCNGC 1 cut(s) 637
GsaI CCCAGC 2 cut(s) 48, 721
HaeII RGCGCY 1 cut(s) 883
HaeIII GGCC 1 cut(s) 643
HapII CCGG 2 cut(s) 49, 473
HhaI GCGC 1 cut(s) 882
Hin1II CATG 1 cut(s) 1064
Hin6I GCGC 1 cut(s) 880
HinP1I GCGC 1 cut(s) 880
HincII GTYRAC 2 cut(s) 238, 284
HindII GTYRAC 2 cut(s) 238, 284
HindIII AAGCTT 1 cut(s) 214
HinfI GANTC 3 cut(s) 280, 302, 1027
HpaII CCGG 2 cut(s) 49, 473
HphI GGTGA 4 cut(s) 134, 899, 944, 1049
Hpy166II GTNNAC 7 cut(s) 145, 238, 284, 510, 655, 771, 797
Hpy188I TCNGA 6 cut(s) 63, 553, 570, 627, 1099, 1218
Hpy188III TCNNGA 4 cut(s) 299, 419, 781, 1048
Hpy8I GTNNAC 7 cut(s) 145, 238, 284, 510, 655, 771, 797
Hpy99I CGWCG 1 cut(s) 63
HpyCH4III ACNGT 3 cut(s) 59, 632, 1214
HpyCH4V TGCA 5 cut(s) 345, 378, 498, 1039, 1117
HpyF10VI GCNNNNNNNGC 1 cut(s) 201
HpyF3I CTNAG 6 cut(s) 230, 437, 483, 600, 645, 666
Hsp92II CATG 1 cut(s) 1064
HspAI GCGC 1 cut(s) 880
KroI GCCGGC 1 cut(s) 48
KroNI GCCGGC 1 cut(s) 50
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 3 cut(s) 256, 548, 980
LmnI GCTCC 5 cut(s) 5, 23, 175, 421, 446
Lsp1109I GCAGC 1 cut(s) 623
LweI GCATC 3 cut(s) 73, 387, 964
MaeI CTAG 7 cut(s) 33, 245, 537, 999, 1073, 1077, 1231
MaeIII GTNAC 4 cut(s) 327, 385, 932, 953
MalI GATC 3 cut(s) 258, 550, 982
MboI GATC 3 cut(s) 256, 548, 980
MboII GAAGA 5 cut(s) 100, 297, 799, 935, 959
MflI RGATCY 1 cut(s) 980
MhlI GDGCHC 3 cut(s) 20, 197, 897
MluCI AATT 5 cut(s) 148, 460, 493, 834, 1080
MlyI GAGTC 2 cut(s) 289, 311
MmeI TCCRAC 2 cut(s) 41, 1196
Mph1103I ATGCAT 1 cut(s) 380
MroNI GCCGGC 1 cut(s) 48
MseI TTAA 3 cut(s) 11, 185, 1068
MslI CAYNNNNRTG 1 cut(s) 1062
MspA1I CMGCKG 1 cut(s) 1208
MspI CCGG 2 cut(s) 49, 473
MspR9I CCNGG 2 cut(s) 333, 884
MvaI CCWGG 2 cut(s) 333, 884
MwoI GCNNNNNNNGC 1 cut(s) 201
NaeI GCCGGC 1 cut(s) 50
NdeI CATATG 1 cut(s) 374
NdeII GATC 3 cut(s) 256, 548, 980
NgoMIV GCCGGC 1 cut(s) 48
NheI GCTAGC 1 cut(s) 536
NlaIII CATG 1 cut(s) 1064
NlaIV GGNNCC 3 cut(s) 7, 19, 982
NmuCI GTSAC 2 cut(s) 932, 953
NsiI ATGCAT 1 cut(s) 380
PdiI GCCGGC 1 cut(s) 50
PfeI GAWTC 1 cut(s) 1027
PflFI GACNNNGTC 1 cut(s) 806
PkrI GCNGC 1 cut(s) 638
PleI GAGTC 2 cut(s) 288, 310
PpsI GAGTC 2 cut(s) 288, 310
PsiI TTATAA 1 cut(s) 699
Psp124BI GAGCTC 1 cut(s) 197
Psp6I CCWGG 2 cut(s) 331, 882
PspFI CCCAGC 2 cut(s) 44, 717
PspGI CCWGG 2 cut(s) 331, 882
PspN4I GGNNCC 3 cut(s) 7, 19, 982
PspPI GGNCC 2 cut(s) 623, 641
PsuI RGATCY 1 cut(s) 980
PsyI GACNNNGTC 1 cut(s) 806
RsaI GTAC 5 cut(s) 253, 511, 683, 1204, 1229
RsaNI GTAC 5 cut(s) 252, 510, 682, 1203, 1228
RseI CAYNNNNRTG 1 cut(s) 1062
SacI GAGCTC 1 cut(s) 197
SaqAI TTAA 3 cut(s) 11, 185, 1068
SatI GCNGC 1 cut(s) 637
Sau3AI GATC 3 cut(s) 256, 548, 980
Sau96I GGNCC 2 cut(s) 623, 641
ScaI AGTACT 1 cut(s) 253
SchI GAGTC 2 cut(s) 289, 311
ScrFI CCNGG 2 cut(s) 333, 884
SduI GDGCHC 3 cut(s) 20, 197, 897
SfaNI GCATC 3 cut(s) 73, 387, 964
SfcI CTRYAG 2 cut(s) 291, 411
SinI GGWCC 1 cut(s) 623
SmiMI CAYNNNNRTG 1 cut(s) 1062
SmlI CTYRAG 3 cut(s) 217, 689, 1048
SmoI CTYRAG 3 cut(s) 217, 689, 1048
Sse9I AATT 5 cut(s) 148, 460, 493, 834, 1080
SsiI CCGC 4 cut(s) 96, 639, 658, 1208
SspMI CTAG 7 cut(s) 33, 245, 537, 999, 1073, 1077, 1231
SstI GAGCTC 1 cut(s) 197
StyD4I CCNGG 2 cut(s) 331, 882
StyI CCWWGG 2 cut(s) 32, 111
TaaI ACNGT 3 cut(s) 59, 632, 1214
TaqI TCGA 2 cut(s) 547, 615
TaqII GACCGA 1 cut(s) 717
TasI AATT 5 cut(s) 148, 460, 493, 834, 1080
TatI WGTACW 3 cut(s) 251, 681, 1227
TfiI GAWTC 1 cut(s) 1027
Tru1I TTAA 3 cut(s) 11, 185, 1068
Tru9I TTAA 3 cut(s) 11, 185, 1068
TscAI CASTG 3 cut(s) 799, 991, 1046
TseFI GTSAC 2 cut(s) 932, 953
TseI GCWGC 1 cut(s) 636
Tsp45I GTSAC 2 cut(s) 932, 953
TspDTI ATGAA 3 cut(s) 408, 800, 869
TspGWI ACGGA 3 cut(s) 82, 95, 802
TspRI CASTG 3 cut(s) 799, 991, 1046
Tth111I GACNNNGTC 1 cut(s) 806
VpaK11BI GGWCC 1 cut(s) 623
XagI CCTNNNNNAGG 1 cut(s) 711
XmaJI CCTAGG 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 770
XspI CTAG 7 cut(s) 33, 245, 537, 999, 1073, 1077, 1231
ZrmI AGTACT 1 cut(s) 253
Zsp2I ATGCAT 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.