RchiOBHm_Chr4g0422881

Mediates both low-affinity uptake and efflux of sugar across the membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004778
Physical Location & Seq
Forward (+)
48455827 .. 48456430
604 bp
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UTR
Exon/CDS
Intron
PRQ39232

Sequence Viewer

Length: 435 bp
ATGGCACAGGTGATCCGCACAGAAAGTGTTGAATTTATGCCGTTCAGCTTATCATTCTTCCTTACATTGAGTGGCATGTTGTGGTTTGCGTATGGACTGTTCCTCAAGGACATACGCATAGCACTTCCAAACGGGTTAGGTTTTGTCTTGGGTCTACTTCAGATGCTGTTCTATGCCATATACAGAAACCGAAAGCTAGTCTTAGTTGATATGGATGAAAACCTTTCGGCATCGGAGCACGTGACAAACGTTGCGATTTTGAGCACTATCGCAACATCCGAAGTTCATTTGGTGAATGCTAAGCCGTGCGATGGCGATGACGGCGAAGATGGAAATAAGGACAGTGATGGCAATTGCGTGGTGGTGGTGGATGTGGATGCAGATGCTTCGGGTGATCACCTTCCACTGAAATCTGATGAATCATGTGTAGTGTGA

Protein Analysis

144

Amino Acids

15.72

Weight (kDa)

4.32

Isoelectric Point (pI)

27.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MtN3_slv PF03083 1 - 65 1.3e-18 Sugar efflux transporter for intercellular exchange
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 154
AciI CCGC 1 cut(s) 16
AclI AACGTT 1 cut(s) 249
AclWI GGATC 1 cut(s) 7
AcsI RAATTY 1 cut(s) 32
AcuI CTGAAG 1 cut(s) 143
AcvI CACGTG 1 cut(s) 241
AfiI CCNNNNNNNGG 1 cut(s) 311
AgsI TTSAA 1 cut(s) 32
AluBI AGCT 2 cut(s) 48, 196
AluI AGCT 2 cut(s) 48, 196
Alw21I GWGCWC 2 cut(s) 240, 266
AlwI GGATC 1 cut(s) 7
AlwNI CAGNNNCTG 1 cut(s) 166
ApoI RAATTY 1 cut(s) 32
AsuHPI GGTGA 4 cut(s) 22, 304, 389, 404
BbrPI CACGTG 1 cut(s) 241
Bbv12I GWGCWC 2 cut(s) 240, 266
BccI CCATC 3 cut(s) 305, 323, 341
BceAI ACGGC 3 cut(s) 25, 289, 337
BcgI CGANNNNNNTGC 2 cut(s) 369, 403
BclI TGATCA 1 cut(s) 394
BfaI CTAG 1 cut(s) 197
BlpI GCTNAGC 1 cut(s) 300
BmsI GCATC 4 cut(s) 153, 239, 367, 373
Bpu1102I GCTNAGC 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 89
BsaAI YACGTR 1 cut(s) 241
Bsc4I CCNNNNNNNGG 1 cut(s) 311
BseGI GGATG 4 cut(s) 220, 275, 376, 382
BseLI CCNNNNNNNGG 1 cut(s) 311
BsiHKAI GWGCWC 2 cut(s) 240, 266
BslI CCNNNNNNNGG 1 cut(s) 311
BsmI GAATGC 1 cut(s) 301
Bsp1286I GDGCHC 2 cut(s) 240, 266
Bsp143I GATC 2 cut(s) 12, 394
Bsp1720I GCTNAGC 1 cut(s) 300
BspACI CCGC 1 cut(s) 16
BspPI GGATC 1 cut(s) 7
BssMI GATC 2 cut(s) 12, 394
Bst4CI ACNGT 2 cut(s) 99, 344
BstBAI YACGTR 1 cut(s) 241
BstDEI CTNAG 2 cut(s) 202, 300
BstF5I GGATG 4 cut(s) 220, 275, 376, 382
BstKTI GATC 2 cut(s) 15, 397
BstMBI GATC 2 cut(s) 12, 394
BstMWI GCNNNNNNNGC 1 cut(s) 321
BstNSI RCATGY 1 cut(s) 79
BtgZI GCGATG 2 cut(s) 324, 330
BtsCI GGATG 4 cut(s) 220, 275, 376, 382
BtsIMutI CAGTG 2 cut(s) 349, 404
CaiI CAGNNNCTG 1 cut(s) 166
CviAII CATG 2 cut(s) 76, 423
CviJI RGCY 3 cut(s) 48, 196, 304
CviKI_1 RGCY 3 cut(s) 48, 196, 304
DdeI CTNAG 2 cut(s) 202, 300
DpnI GATC 2 cut(s) 14, 396
DpnII GATC 2 cut(s) 12, 394
Eco57I CTGAAG 1 cut(s) 143
Eco72I CACGTG 1 cut(s) 241
FaeI CATG 2 cut(s) 79, 426
FalI AAGNNNNNCTT 2 cut(s) 185, 217
FatI CATG 2 cut(s) 75, 422
FbaI TGATCA 1 cut(s) 394
FblI GTMKAC 1 cut(s) 154
FokI GGATG 4 cut(s) 227, 262, 383, 389
FspBI CTAG 1 cut(s) 197
Hin1II CATG 2 cut(s) 79, 426
HinfI GANTC 1 cut(s) 419
HphI GGTGA 4 cut(s) 22, 304, 389, 404
Hpy166II GTNNAC 1 cut(s) 155
Hpy188I TCNGA 4 cut(s) 162, 235, 280, 415
Hpy8I GTNNAC 1 cut(s) 155
HpyAV CCTTC 1 cut(s) 410
HpyCH4III ACNGT 2 cut(s) 99, 344
HpyCH4IV ACGT 2 cut(s) 240, 249
HpyCH4V TGCA 1 cut(s) 380
HpyF10VI GCNNNNNNNGC 1 cut(s) 321
HpyF3I CTNAG 2 cut(s) 202, 300
HpySE526I ACGT 2 cut(s) 240, 249
Hsp92II CATG 2 cut(s) 79, 426
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 2 cut(s) 12, 394
LmnI GCTCC 1 cut(s) 235
LweI GCATC 4 cut(s) 153, 239, 367, 373
MaeI CTAG 1 cut(s) 197
MaeII ACGT 2 cut(s) 240, 249
MaeIII GTNAC 1 cut(s) 241
MalI GATC 2 cut(s) 14, 396
MboI GATC 2 cut(s) 12, 394
MboII GAAGA 2 cut(s) 49, 338
MfeI CAATTG 1 cut(s) 352
MhlI GDGCHC 2 cut(s) 240, 266
MluCI AATT 2 cut(s) 32, 352
MnlI CCTC 1 cut(s) 113
MunI CAATTG 1 cut(s) 352
Mva1269I GAATGC 1 cut(s) 301
MwoI GCNNNNNNNGC 1 cut(s) 321
NdeII GATC 2 cut(s) 12, 394
NlaIII CATG 2 cut(s) 79, 426
NmuCI GTSAC 1 cut(s) 241
NspI RCATGY 1 cut(s) 79
PcsI WCGNNNNNNNCGW 2 cut(s) 246, 276
PctI GAATGC 1 cut(s) 301
PfeI GAWTC 1 cut(s) 419
PmaCI CACGTG 1 cut(s) 241
PmlI CACGTG 1 cut(s) 241
Ppu21I YACGTR 1 cut(s) 241
Psp1406I AACGTT 1 cut(s) 249
PspCI CACGTG 1 cut(s) 241
PsrI GAACNNNNNNTAC 2 cut(s) 83, 115
PstNI CAGNNNCTG 1 cut(s) 166
Sau3AI GATC 2 cut(s) 12, 394
SduI GDGCHC 2 cut(s) 240, 266
SetI ASST 8 cut(s) 12, 50, 142, 198, 225, 243, 252, 402
SfaNI GCATC 4 cut(s) 153, 239, 367, 373
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 2 cut(s) 32, 352
SsiI CCGC 1 cut(s) 16
SspMI CTAG 1 cut(s) 197
TaaI ACNGT 2 cut(s) 99, 344
TaiI ACGT 2 cut(s) 243, 252
TasI AATT 2 cut(s) 32, 352
TfiI GAWTC 1 cut(s) 419
TscAI CASTG 2 cut(s) 349, 411
TseFI GTSAC 1 cut(s) 241
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 3 cut(s) 231, 275, 432
TspRI CASTG 2 cut(s) 349, 411
XapI RAATTY 1 cut(s) 32
XceI RCATGY 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 154
XspI CTAG 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.