Rorug04G0236500

Mediates both low-affinity uptake and efflux of sugar across the membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004778
Physical Location & Seq
Forward (+)
40282919 .. 40286557
3639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0236500.1

Sequence Viewer

Length: 3552 bp
ATGCATGTACTCTCTGTCCTTACTCCCGTTGCCGGAGGAGTAGTGAGTAAGGTGGCTTTACTTGCTACTGATCAATTGAGACTCGAAATCGTCTGCTGTTTCCGCAAAGAGCTAAATAAGCTCAAAGAATCCTCAATTCTGATTGGAAATATAATCCATGATGCTGCTCACGAACCTGAAGATGGGGATCTGCGGGCAAGGGCCGAGTGGATGAAGAGGCTGATAAGCGTAGCTTATGATGCAGAAGATATCCTGGATAAATTTCAATATGAAGTTTATCGGATCGTTATAAAGGAGACAAGCCTCGACAAGAAGATACTCGGCTTCTTCTGCAACAATCCTCTTGTATTTCGTCTCAAGATGGCCCGCAAGATTTACAACATCAACACATCCTTGGAGGATCTCAACAAGCAAGCACCTGGCGTGGGACTCGTCAGATCAAATGGAGGGGCAGCAGCCAGTCAAGGTATGCAGGACAGGGAAACAACCTCATTCCCAGAGAAAAATGAATTCACCTTCAAAGATGCAATCACTGTTGGAAGGGATCAGGCTGTGTCAGATATAATTACAACCTTTACCAACTCCAACAATCAAGAAAATATTCTTTCGGTGATGGCCATTGTGGGATTGGGAGGCCTAGGTAAAACAACTTTGGCTCGCTTAATTACCAAGCAACTGAAAGAAGGTGAAATGGGAAAGCACTTTGATACAACGCTCTGGGTGTACGTATCTAATACTTTTGATGTCAATTCAATTTTACGTCTAATGCTGGAGTCATGCGGCGCGACGGGGGCAAATCTTTCAAGTCGGGAAGCATTGATTGAAAGCCTCCGCCATAAGTTGAAAGAGAAAAGGTATTTTCTTGTACTTGATGATGTTTGGAATGAAGATGCTAATAATTGGAATGATTTGAAGAGTTGTTTGTTGCAACTAAATTCTGCCAAAGGAAGCTCTGTGATTGTCACCACCCGTAGATCCAATGTTGCATCAATCATGGAAACACTTCCTAGGTGGAATTTAGAAACTTTATCGGATGATGAATGTTGGTCCATAATGAAGGATAGAGCATTTGCAGATCCTAATGCTCCCATTGCTTCAGAACTAGAGGAAATTGGAAGGGAAATTGCCAAAAAGTGTGCTGGTCTTCCATTGGCGGCAAAGGTTTTGGGAAGCTTGATGCGTTCTAAGAACAGTATGAAGGAATGGTTGTCAATTCTAGAAAGTCAAATATGGCAGTTATCATCCAACAATGAGGATAACAGGATCATGTCGGTTTTGAAGTTGAGTTTTGACAATTTGAAATCACCACTGAAACAATGTTTTGCATATTGCTCTATGTTCAAGAGAGGTTACTCAATTGAAAGAGATAAGTTGATCCAACTATGGATGGCTCAAGGTTTTCTCCATCCTTCTTATGGTCCCCAAACCAAGCAAGAGATTGAAATGGAGGATATAGGCAATAAGTATTATGAGACTCTACTGGAGAACTCCTTATTTCAAGATGCTACAAAGGATAAGGATGGTGTTATCATCACCCAATGCAAGATGCATGATCTTGTGCATGATCTTGCAAACGAAGTATCCATGTGTGAGAGCTTGACACCGGACTTCACTCATGAGAAGAATGATCATGATATACTTATTCGACATGTTGCACGGATTCCTACTCCAACACTAGAAGAAATGTCAGAAAGAAGTATTTCAAGACTGCGATCACTCTTTTCAAATGACCCTGTCTCTAATACCATTTTCCAAAAGCTTACAAGTTTACGTGTCTTAAGTTTCTCTAGTTATGAGATTGAGAAGTTGCCAAATTTACTCGGCAAGCTGAAGCACTTGAGGTATCTAGATTTTTCCAGTACAAACATTGAAACACTCCCAAAGTCTATTGGCAAGCTCTATAACCTCCAAACATTGAGATTGCCGCTGGGTCTCAAAGAGTGTCCAAAGGAGATTCAAAATTTGATCAACTTGAGGCATTTTTATTTTGGTAAGGAAATGAAATTTGAGGCTGGGAGTTTGGGGCGATTAACTAATCTCAGAAGATTGCCTCCCTTTAATGTGGGTAAGGAGAGAGGTCTTGCAATCAAGGAGTTGGGTGGGTTGAACCAATTGAGAGGCCACTTAATTATTGATGGGCTAGAACATGTTAGGGATGGAGAAGAAGCAAAGAAGGCTTTCTTAGTGGAGAAGAGTCACTTGAGCAAGCTAACATTTAAATGGACATCCCAGCGACCTAATGTAAGTGAGAAGGATGTACTAGATGGCTTGGAACCTCATGGTAACTTGCAAAGCTTGAAAATTAAGAATTTCACTGGTGCCAGATTTCCGTCATGGATAATGAGTCTCCAAAATTTGAAGCAGATTCGATTAGTCTACTGCAACAATAGTGTGGGAGCTGACTTTTATGGTACTAGTACAACTTTATTTCCGGCACTGGAAACATTAGACATCCGTGCCTGCGAAGAGCTCATTGAATGGATGGAAGCACCGAGGATATCAGCTGAAGGAGAAGTCGTGGTATTTCCCTGCCTTCGATCAATGAAAATTACGGGGTGTAATAAGCTGAGGCATTTGCCGCATGGGCTAGACAGACTCACTCCTCTTGAGACATTGAGAATAAATCATTGTAACGGCCTAGAGTCGATTCAGATCACACACGGCATCGCATCTCTCCGTCAATTGAACATCTCTAATTGTTGGAGATTATCAAGCCTAGAGGTTGGGCTCGATTACTGCACCTTTCTTCAGGAGTTGCATATATATGGTTGCCCTAATCTAATGTCAATTCCAACCACACATGGCATGCCGTCTCTCCGGAAATTGACGATTAATGAGTGTGGGGGATTGTCAAGCCTAGGAAGTGGGCTCAACTACTGCACCTCTCTTCAGGAATTGGAGATTGAGAGATGTCAGAATCTAACATCAATTCCAATCACACAGGGCATCACATCTCTCCGGAGCTTGACGATTGTTTCTTGTCAGAGATTATTAAGCCTACCGAGTGGGCTCCAATTCTGCACCTCTCTTCAGCATCTGGAAATTGGTCTGTTCTGGAAGGAGCTGGACGCCTTCCCTGATTTTCAGCTTCCTCCACCACATGATTCACAACTTGAAGACTTAAAGCTGACAGGGTGGCCTAAGCTCAAGTCTCTGCCCCAACAAATTCAGCACTTGACATGTTTAAAAGAACTCTGGATAAGTTATTTTGAGGGACTAGAGGCTCTTCCTGAGTGGTTGGGAAATCTTACATCTCTTGAGTCTCTGAGAATCTCATACTGTGAGAATCTCAAGTATCTACCTACACAAAATTCTATGAAAAACCTCAACAAATTGAAGACGCTCTACATTGATGATGGATGTCCCCTGCTCAAAAAAGGATGCACCAAGGAGACTGGCCCAGAATGGCCCAAGATTTCTCACATTCCAAAAGTTTCCAGTAATTGTTTTGACCAAATCGATCTGTTTTCTTTTTATTTTCTTTTTTCCTACAAATTTCTTTCTTATTATGTCTCATTTTTCATCTTTCTTAACTCAGTCTCAAGATGGTAA

Protein Analysis

1183

Amino Acids

134.16

Weight (kDa)

7.11

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 14 - 93 2.6e-09 Rx N-terminal domain
NB-ARC PF00931 183 - 359 1.6e-32 NB-ARC domain
AAA_22 PF13401 204 - 321 2.2e-08 AAA domain
NACHT PF05729 204 - 341 6.3e-06 NACHT domain
WHD_DRP PF23559 446 - 524 1.1e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 570 - 851 1.3e-30 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 696 - 820 7.8e-30 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 776 - 900 2.9e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 841 - 972 1.2e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_14 PF23598 1038 - 1117 1.7e-06 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 1056 - 1117 5.9e-07 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 290
AccB1I GGYRCC 1 cut(s) 2318
AccI GTMKAC 1 cut(s) 2376
AccII CGCG 1 cut(s) 785
AccIII TCCGGA 2 cut(s) 2817, 2958
AciI CCGC 8 cut(s) 103, 193, 367, 780, 832, 1154, 1925, 2579
AclWI GGATC 8 cut(s) 195, 290, 408, 552, 969, 1070, 1271, 1369
AcoI YGGCCR 1 cut(s) 615
AcuI CTGAAG 7 cut(s) 198, 1080, 1850, 2526, 2732, 2873, 3014
AcyI GRCGYC 1 cut(s) 3069
AfaI GTAC 7 cut(s) 9, 725, 867, 1861, 2259, 2413, 2419
AfiI CCNNNNNNNGG 4 cut(s) 32, 182, 425, 1415
AflII CTTAAG 1 cut(s) 1777
AflIII ACRYGT 4 cut(s) 1648, 1771, 2146, 3179
AhlI ACTAGT 1 cut(s) 2414
AjnI CCWGG 2 cut(s) 252, 418
Alw21I GWGCWC 1 cut(s) 2472
AlwI GGATC 8 cut(s) 195, 290, 408, 552, 969, 1070, 1271, 1369
AlwNI CAGNNNCTG 1 cut(s) 3037
Aor13HI TCCGGA 2 cut(s) 2817, 2958
AoxI GGCC 9 cut(s) 201, 363, 615, 634, 2119, 2635, 3137, 3397, 3407
ApeKI GCWGC 3 cut(s) 164, 452, 455
AseI ATTAAT 1 cut(s) 2832
Asp700I GAANNNNTTC 4 cut(s) 600, 1002, 1699, 2177
AspA2I CCTAGG 3 cut(s) 637, 1007, 2857
AspLEI GCGC 1 cut(s) 785
AspS9I GGNCC 6 cut(s) 201, 364, 1047, 1418, 3398, 3408
AsuHPI GGTGA 6 cut(s) 505, 622, 698, 955, 1296, 1525
AvaII GGWCC 2 cut(s) 1047, 1418
AvrII CCTAGG 3 cut(s) 637, 1007, 2857
BalI TGGCCA 1 cut(s) 617
BanI GGYRCC 1 cut(s) 2318
BanII GRGCYC 4 cut(s) 2472, 2730, 2871, 3012
BarI GAAGNNNNNNTAC 2 cut(s) 3329, 3361
BbsI GAAGAC 3 cut(s) 1136, 3123, 3344
Bbv12I GWGCWC 1 cut(s) 2472
BbvCI CCTCAGC 1 cut(s) 2567
BbvI GCAGC 3 cut(s) 151, 464, 467
BceAI ACGGC 3 cut(s) 2650, 2677, 2794
BciT130I CCWGG 2 cut(s) 254, 420
BciVI GTATCC 1 cut(s) 1591
BclI TGATCA 3 cut(s) 70, 1627, 1965
BcuI ACTAGT 1 cut(s) 2414
BfrI CTTAAG 1 cut(s) 1777
BfuI GTATCC 1 cut(s) 1591
BglI GCCNNNNNGGC 1 cut(s) 2584
BisI GCNGC 7 cut(s) 165, 453, 456, 781, 1155, 1925, 2579
BlnI CCTAGG 3 cut(s) 637, 1007, 2857
BlsI GCNGC 7 cut(s) 166, 454, 457, 782, 1156, 1926, 2580
Bme1390I CCNGG 2 cut(s) 254, 420
Bme18I GGWCC 2 cut(s) 1047, 1418
BmgT120I GGNCC 6 cut(s) 201, 364, 1047, 1418, 3398, 3408
BmiI GGNNCC 4 cut(s) 1420, 2274, 2320, 3011
BmrFI CCNGG 2 cut(s) 254, 420
BpiI GAAGAC 3 cut(s) 1136, 3123, 3344
BpmI CTGGAG 2 cut(s) 791, 1502
Bpu10I CCTNAGC 2 cut(s) 2567, 3141
Bsa29I ATCGAT 1 cut(s) 3459
BsaAI YACGTR 2 cut(s) 727, 1772
BsaBI GATNNNNATC 2 cut(s) 186, 2651
BsaHI GRCGYC 1 cut(s) 3069
BsaI GGTCTC 1 cut(s) 1937
BsaJI CCNNGG 6 cut(s) 393, 637, 1007, 2492, 2857, 3387
BsaWI WCCGGW 3 cut(s) 1603, 2817, 2958
BsaXI ACNNNNNCTCC 4 cut(s) 27, 57, 2502, 2532
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 182, 425, 1415
Bse1I ACTGG 7 cut(s) 459, 1485, 1857, 2320, 2442, 3400, 3438
Bse3DI GCAATG 1 cut(s) 1089
Bse8I GATNNNNATC 2 cut(s) 186, 2651
BseAI TCCGGA 2 cut(s) 2817, 2958
BseBI CCWGG 2 cut(s) 254, 420
BseCI ATCGAT 1 cut(s) 3459
BseDI CCNNGG 6 cut(s) 393, 637, 1007, 2492, 2857, 3387
BseJI GATNNNNATC 2 cut(s) 186, 2651
BseLI CCNNNNNNNGG 4 cut(s) 32, 182, 425, 1415
BseMI GCAATG 1 cut(s) 1089
BseMII CTCAG 5 cut(s) 2053, 2558, 3222, 3257, 3549
BseNI ACTGG 7 cut(s) 459, 1485, 1857, 2320, 2442, 3400, 3438
BseRI GAGGAG 2 cut(s) 51, 2592
BseXI GCAGC 3 cut(s) 151, 464, 467
BseYI CCCAGC 3 cut(s) 1927, 2012, 2229
BsgI GTGCAG 3 cut(s) 2722, 2863, 3004
Bsh1236I CGCG 1 cut(s) 785
BshFI GGCC 9 cut(s) 203, 365, 617, 636, 2121, 2637, 3139, 3399, 3409
BshNI GGYRCC 1 cut(s) 2318
BshVI ATCGAT 1 cut(s) 3459
BsiHKAI GWGCWC 1 cut(s) 2472
BsiSI CCGG 5 cut(s) 33, 1604, 2432, 2818, 2959
BslFI GGGAC 4 cut(s) 441, 1404, 3228, 3348
BslI CCNNNNNNNGG 4 cut(s) 32, 182, 425, 1415
BsmBI CGTCTC 2 cut(s) 359, 2817
BsmFI GGGAC 4 cut(s) 441, 1404, 3228, 3348
BsnI GGCC 9 cut(s) 203, 365, 617, 636, 2121, 2637, 3139, 3399, 3409
Bso31I GGTCTC 1 cut(s) 1937
Bsp1286I GDGCHC 4 cut(s) 2472, 2730, 2871, 3012
Bsp13I TCCGGA 2 cut(s) 2817, 2958
BspACI CCGC 8 cut(s) 103, 193, 367, 780, 832, 1154, 1925, 2579
BspANI GGCC 9 cut(s) 203, 365, 617, 636, 2121, 2637, 3139, 3399, 3409
BspCNI CTCAG 5 cut(s) 2052, 2559, 3223, 3258, 3548
BspDI ATCGAT 1 cut(s) 3459
BspEI TCCGGA 2 cut(s) 2817, 2958
BspFNI CGCG 1 cut(s) 785
BspHI TCATGA 2 cut(s) 1615, 1630
BspLI GGNNCC 4 cut(s) 1420, 2274, 2320, 3011
BspPI GGATC 8 cut(s) 195, 290, 408, 552, 969, 1070, 1271, 1369
BspQI GCTCTTC 2 cut(s) 2460, 3231
BspT107I GGYRCC 1 cut(s) 2318
BspTI CTTAAG 1 cut(s) 1777
BspTNI GGTCTC 1 cut(s) 1937
BsrDI GCAATG 1 cut(s) 1089
BsrI ACTGG 7 cut(s) 459, 1485, 1857, 2320, 2442, 3400, 3438
BssECI CCNNGG 6 cut(s) 393, 637, 1007, 2492, 2857, 3387
BssNI GRCGYC 1 cut(s) 3069
BssT1I CCWWGG 5 cut(s) 393, 637, 1007, 2857, 3387
Bst2UI CCWGG 2 cut(s) 254, 420
Bst4CI ACNGT 3 cut(s) 535, 1193, 3281
Bst6I CTCTTC 7 cut(s) 209, 908, 2186, 2460, 2892, 3033, 3231
BstACI GRCGYC 1 cut(s) 3069
BstAFI CTTAAG 1 cut(s) 1777
BstBAI YACGTR 2 cut(s) 727, 1772
BstC8I GCNNGC 9 cut(s) 195, 367, 414, 658, 1826, 1895, 2207, 2461, 2807
BstDEI CTNAG 8 cut(s) 1185, 2039, 2182, 2567, 3141, 3231, 3266, 3535
BstFNI CGCG 1 cut(s) 785
BstHHI GCGC 1 cut(s) 785
BstNI CCWGG 2 cut(s) 254, 420
BstNSI RCATGY 5 cut(s) 8, 1652, 2150, 2809, 3183
BstSCI CCNGG 2 cut(s) 252, 418
BstSNI TACGTA 1 cut(s) 727
BstUI CGCG 1 cut(s) 785
BstV1I GCAGC 3 cut(s) 151, 464, 467
BstV2I GAAGAC 3 cut(s) 1136, 3123, 3344
BstX2I RGATCY 4 cut(s) 187, 400, 974, 1075
BstYI RGATCY 4 cut(s) 187, 400, 974, 1075
Bsu15I ATCGAT 1 cut(s) 3459
BsuI GTATCC 1 cut(s) 1591
BsuRI GGCC 9 cut(s) 203, 365, 617, 636, 2121, 2637, 3139, 3399, 3409
BsuTUI ATCGAT 1 cut(s) 3459
BtgZI GCGATG 1 cut(s) 2650
BtsIMutI CAGTG 4 cut(s) 531, 1307, 2313, 2435
Cac8I GCNNGC 9 cut(s) 195, 367, 414, 658, 1826, 1895, 2207, 2461, 2807
CaiI CAGNNNCTG 1 cut(s) 3037
CciI TCATGA 2 cut(s) 1615, 1630
CfoI GCGC 1 cut(s) 785
Cfr13I GGNCC 6 cut(s) 201, 364, 1047, 1418, 3398, 3408
ClaI ATCGAT 1 cut(s) 3459
CseI GACGC 2 cut(s) 3077, 3349
Csp6I GTAC 7 cut(s) 8, 724, 866, 1860, 2258, 2412, 2418
CviQI GTAC 7 cut(s) 8, 724, 866, 1860, 2258, 2412, 2418
DdeI CTNAG 8 cut(s) 1185, 2039, 2182, 2567, 3141, 3231, 3266, 3535
DraI TTTAAA 2 cut(s) 2218, 3186
EaeI YGGCCR 1 cut(s) 615
Eam1104I CTCTTC 7 cut(s) 209, 908, 2186, 2460, 2892, 3033, 3231
EarI CTCTTC 7 cut(s) 209, 908, 2186, 2460, 2892, 3033, 3231
EciI GGCGGA 1 cut(s) 821
Ecl136II GAGCTC 1 cut(s) 2470
Eco105I TACGTA 1 cut(s) 727
Eco130I CCWWGG 5 cut(s) 393, 637, 1007, 2857, 3387
Eco147I AGGCCT 1 cut(s) 636
Eco24I GRGCYC 4 cut(s) 2472, 2730, 2871, 3012
Eco31I GGTCTC 1 cut(s) 1937
Eco32I GATATC 2 cut(s) 250, 2499
Eco47I GGWCC 2 cut(s) 1047, 1418
Eco53kI GAGCTC 1 cut(s) 2470
Eco57I CTGAAG 7 cut(s) 198, 1080, 1850, 2526, 2732, 2873, 3014
EcoICRI GAGCTC 1 cut(s) 2470
EcoRI GAATTC 1 cut(s) 509
EcoRII CCWGG 2 cut(s) 252, 418
EcoRV GATATC 2 cut(s) 250, 2499
EcoT14I CCWWGG 5 cut(s) 393, 637, 1007, 2857, 3387
EcoT22I ATGCAT 2 cut(s) 6, 1551
EcoT38I GRGCYC 4 cut(s) 2472, 2730, 2871, 3012
ErhI CCWWGG 5 cut(s) 393, 637, 1007, 2857, 3387
Esp3I CGTCTC 2 cut(s) 359, 2817
FalI AAGNNNNNCTT 6 cut(s) 217, 249, 2165, 2197, 2183, 2215
FaqI GGGAC 4 cut(s) 441, 1404, 3228, 3348
FauI CCCGC 2 cut(s) 186, 374
FbaI TGATCA 3 cut(s) 70, 1627, 1965
FblI GTMKAC 1 cut(s) 2376
Fnu4HI GCNGC 7 cut(s) 165, 453, 456, 781, 1155, 1925, 2579
FriOI GRGCYC 4 cut(s) 2472, 2730, 2871, 3012
Fsp4HI GCNGC 7 cut(s) 165, 453, 456, 781, 1155, 1925, 2579
GlaI GCGC 1 cut(s) 784
GluI GCNGC 7 cut(s) 165, 453, 456, 781, 1155, 1925, 2579
GsaI CCCAGC 3 cut(s) 1931, 2016, 2233
GsuI CTGGAG 2 cut(s) 791, 1502
HaeIII GGCC 9 cut(s) 203, 365, 617, 636, 2121, 2637, 3139, 3399, 3409
HapII CCGG 5 cut(s) 33, 1604, 2432, 2818, 2959
HgaI GACGC 2 cut(s) 3077, 3349
HhaI GCGC 1 cut(s) 785
Hin1I GRCGYC 1 cut(s) 3069
Hin6I GCGC 1 cut(s) 783
HinP1I GCGC 1 cut(s) 783
HindIII AAGCTT 3 cut(s) 1171, 1757, 2293
HpaII CCGG 5 cut(s) 33, 1604, 2432, 2818, 2959
HphI GGTGA 6 cut(s) 505, 622, 698, 955, 1296, 1525
Hpy166II GTNNAC 3 cut(s) 724, 1769, 2377
Hpy8I GTNNAC 3 cut(s) 724, 1769, 2377
Hpy99I CGWCG 1 cut(s) 790
HpyCH4III ACNGT 3 cut(s) 535, 1193, 3281
HpyCH4IV ACGT 3 cut(s) 726, 760, 1771
HpyF3I CTNAG 8 cut(s) 1185, 2039, 2182, 2567, 3141, 3231, 3266, 3535
HpySE526I ACGT 3 cut(s) 726, 760, 1771
Hsp92I GRCGYC 1 cut(s) 3069
HspAI GCGC 1 cut(s) 783
Kpn2I TCCGGA 2 cut(s) 2817, 2958
Ksp22I TGATCA 3 cut(s) 70, 1627, 1965
LguI GCTCTTC 2 cut(s) 2460, 3231
LmnI GCTCC 5 cut(s) 1090, 2396, 2961, 3015, 3061
Lsp1109I GCAGC 3 cut(s) 151, 464, 467
MaeII ACGT 3 cut(s) 726, 760, 1771
MaeIII GTNAC 5 cut(s) 961, 1349, 2195, 2282, 2630
MfeI CAATTG 4 cut(s) 74, 1356, 2111, 2681
MflI RGATCY 4 cut(s) 187, 400, 974, 1075
MhlI GDGCHC 4 cut(s) 2472, 2730, 2871, 3012
MlsI TGGCCA 1 cut(s) 617
MluNI TGGCCA 1 cut(s) 617
MlyI GAGTC 9 cut(s) 75, 423, 782, 1468, 2203, 2353, 2589, 2651, 3269
MmeI TCCRAC 7 cut(s) 517, 609, 1269, 1402, 1694, 2681, 2816
Mox20I TGGCCA 1 cut(s) 617
Mph1103I ATGCAT 2 cut(s) 6, 1551
MroI TCCGGA 2 cut(s) 2817, 2958
MroXI GAANNNNTTC 4 cut(s) 600, 1002, 1699, 2177
MscI TGGCCA 1 cut(s) 617
MslI CAYNNNNRTG 2 cut(s) 2218, 2763
Msp20I TGGCCA 1 cut(s) 617
MspA1I CMGCKG 2 cut(s) 1927, 2504
MspCI CTTAAG 1 cut(s) 1777
MspI CCGG 5 cut(s) 33, 1604, 2432, 2818, 2959
MspR9I CCNGG 2 cut(s) 254, 420
MunI CAATTG 4 cut(s) 74, 1356, 2111, 2681
MvaI CCWGG 2 cut(s) 254, 420
MvnI CGCG 1 cut(s) 785
NlaIV GGNNCC 4 cut(s) 1420, 2274, 2320, 3011
NmeAIII GCCGAG 3 cut(s) 229, 300, 1800
NmuCI GTSAC 2 cut(s) 961, 2195
NsiI ATGCAT 2 cut(s) 6, 1551
NspI RCATGY 5 cut(s) 8, 1652, 2150, 2809, 3183
PaeI GCATGC 1 cut(s) 2809
PagI TCATGA 2 cut(s) 1615, 1630
PceI AGGCCT 1 cut(s) 636
PciI ACATGT 3 cut(s) 1648, 2146, 3179
PciSI GCTCTTC 2 cut(s) 2460, 3231
PdmI GAANNNNTTC 4 cut(s) 600, 1002, 1699, 2177
PfeI GAWTC 9 cut(s) 128, 1660, 1954, 2365, 2647, 2917, 3104, 3270, 3286
PflFI GACNNNGTC 1 cut(s) 1733
PfoI TCCNGGA 1 cut(s) 252
PkrI GCNGC 7 cut(s) 166, 454, 457, 782, 1156, 1926, 2580
PleI GAGTC 9 cut(s) 75, 423, 781, 1468, 2202, 2352, 2589, 2650, 3268
PpsI GAGTC 9 cut(s) 75, 423, 781, 1468, 2202, 2352, 2589, 2650, 3268
Ppu21I YACGTR 2 cut(s) 727, 1772
PscI ACATGT 3 cut(s) 1648, 2146, 3179
PshBI ATTAAT 1 cut(s) 2832
PsiI TTATAA 1 cut(s) 290
Psp124BI GAGCTC 1 cut(s) 2472
Psp6I CCWGG 2 cut(s) 252, 418
PspFI CCCAGC 3 cut(s) 1927, 2012, 2229
PspGI CCWGG 2 cut(s) 252, 418
PspN4I GGNNCC 4 cut(s) 1420, 2274, 2320, 3011
PspPI GGNCC 6 cut(s) 201, 364, 1047, 1418, 3398, 3408
PstNI CAGNNNCTG 1 cut(s) 3037
PsuI RGATCY 4 cut(s) 187, 400, 974, 1075
PsyI GACNNNGTC 1 cut(s) 1733
PvuII CAGCTG 1 cut(s) 2504
RsaI GTAC 7 cut(s) 9, 725, 867, 1861, 2259, 2413, 2419
RsaNI GTAC 7 cut(s) 8, 724, 866, 1860, 2258, 2412, 2418
RseI CAYNNNNRTG 2 cut(s) 2218, 2763
SacI GAGCTC 1 cut(s) 2472
SapI GCTCTTC 2 cut(s) 2460, 3231
SatI GCNGC 7 cut(s) 165, 453, 456, 781, 1155, 1925, 2579
Sau96I GGNCC 6 cut(s) 201, 364, 1047, 1418, 3398, 3408
SchI GAGTC 9 cut(s) 75, 423, 782, 1468, 2203, 2353, 2589, 2651, 3269
ScrFI CCNGG 2 cut(s) 254, 420
SduI GDGCHC 4 cut(s) 2472, 2730, 2871, 3012
SinI GGWCC 2 cut(s) 1047, 1418
SmiI ATTTAAAT 1 cut(s) 2218
SmiMI CAYNNNNRTG 2 cut(s) 2218, 2763
SnaBI TACGTA 1 cut(s) 727
SpeI ACTAGT 1 cut(s) 2414
SphI GCATGC 1 cut(s) 2809
SseBI AGGCCT 1 cut(s) 636
SsiI CCGC 8 cut(s) 103, 193, 367, 780, 832, 1154, 1925, 2579
SspI AATATT 1 cut(s) 601
SstI GAGCTC 1 cut(s) 2472
StuI AGGCCT 1 cut(s) 636
StyD4I CCNGG 2 cut(s) 252, 418
StyI CCWWGG 5 cut(s) 393, 637, 1007, 2857, 3387
SwaI ATTTAAAT 1 cut(s) 2218
TaaI ACNGT 3 cut(s) 535, 1193, 3281
TaiI ACGT 3 cut(s) 729, 763, 1774
TaqI TCGA 8 cut(s) 84, 306, 1645, 2368, 2536, 2645, 2730, 3459
TatI WGTACW 5 cut(s) 7, 865, 1859, 2257, 2417
TauI GCSGC 4 cut(s) 783, 1157, 1927, 2581
TfiI GAWTC 9 cut(s) 128, 1660, 1954, 2365, 2647, 2917, 3104, 3270, 3286
TscAI CASTG 4 cut(s) 538, 1314, 2320, 2442
TseFI GTSAC 2 cut(s) 961, 2195
TseI GCWGC 3 cut(s) 164, 452, 455
Tsp45I GTSAC 2 cut(s) 961, 2195
TspGWI ACGGA 4 cut(s) 1672, 2319, 2444, 2666
TspRI CASTG 4 cut(s) 538, 1314, 2320, 2442
Tth111I GACNNNGTC 1 cut(s) 1733
Vha464I CTTAAG 1 cut(s) 1777
VpaK11BI GGWCC 2 cut(s) 1047, 1418
VspI ATTAAT 1 cut(s) 2832
XbaI TCTAGA 2 cut(s) 1216, 1846
XceI RCATGY 5 cut(s) 8, 1652, 2150, 2809, 3183
XcmI CCANNNNNNNNNTGG 2 cut(s) 625, 1412
XmaJI CCTAGG 3 cut(s) 637, 1007, 2857
XmiI GTMKAC 1 cut(s) 2376
XmnI GAANNNNTTC 4 cut(s) 600, 1002, 1699, 2177
Zsp2I ATGCAT 2 cut(s) 6, 1551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.