Rh4DG241100

Mediates both low-affinity uptake and efflux of sugar across the membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004778
Physical Location & Seq
Forward (+)
44967443 .. 44968437
995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG241100.1

Sequence Viewer

Length: 549 bp
ATGCTGAAGCAAAATGTTGTGCTTCTCATCACAGTTAACACCTTCGGAAGTGTGATGGAGACTGTGTACATCGCCATGTACATTCTTTATGCAACAAACACTTCAAGGGCACAGGTCATCCGCACAGAAAGTGTTGAATTTATGCCGTTCAGCTTATCATTCTTCCTTACATTGAGTGGCATGTTGTGGTTTGCGTATGGACTGTTCCTCAAGGACATACGCATAGCACTTCCAAACGGGTTAGGTTTTGTCTTGGGTCTACTTCAGATGTTGTTCTATGCCATATACAGAAACCGAAAGCTAGTCTTAGTTGATATGGATGAAAACCTTCCGGCACCGGAGCATGTGACAAACGTTGTGATTCTGAGCACTATCGTAACATCCGAAGTTCATTCGGTGAATGCTAAGCCGTGCGATGGCGATGACGTCGAAGACGGCAAAGATGGAAATAAGGACAGTGATGGCGATTGTGTGGTGGTGGTGGATGTGGATGCGGATGCTTCGGGTGATCACCTTCCACTGAAATCTGATGAATCATGTGTAGTGTGA

Protein Analysis

182

Amino Acids

20.06

Weight (kDa)

4.42

Isoelectric Point (pI)

29.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MtN3_slv PF03083 38 - 100 4.4e-18 Sugar efflux transporter for intercellular exchange
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 429
AccB1I GGYRCC 1 cut(s) 334
AccI GTMKAC 1 cut(s) 259
AciI CCGC 2 cut(s) 121, 494
AclI AACGTT 1 cut(s) 354
AcsI RAATTY 1 cut(s) 137
AcuI CTGAAG 2 cut(s) 26, 248
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 2 cut(s) 68, 80
AfiI CCNNNNNNNGG 1 cut(s) 416
AgsI TTSAA 2 cut(s) 105, 137
AluBI AGCT 2 cut(s) 153, 301
AluI AGCT 2 cut(s) 153, 301
Alw21I GWGCWC 1 cut(s) 371
Alw26I GTCTC 1 cut(s) 53
ApoI RAATTY 1 cut(s) 137
Asp700I GAANNNNTTC 1 cut(s) 327
AsuHPI GGTGA 3 cut(s) 409, 503, 518
BaeGI GKGCMC 1 cut(s) 112
BanI GGYRCC 1 cut(s) 334
BbsI GAAGAC 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 371
BccI CCATC 4 cut(s) 49, 410, 437, 455
BceAI ACGGC 3 cut(s) 130, 394, 451
BclI TGATCA 1 cut(s) 508
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 1 cut(s) 302
BlpI GCTNAGC 1 cut(s) 405
BmiI GGNNCC 1 cut(s) 336
BmsI GCATC 2 cut(s) 481, 487
BpiI GAAGAC 1 cut(s) 438
Bpu1102I GCTNAGC 1 cut(s) 405
BpuEI CTTGAG 1 cut(s) 194
BsaHI GRCGYC 1 cut(s) 426
BsaWI WCCGGW 1 cut(s) 337
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 1 cut(s) 416
BseGI GGATG 6 cut(s) 117, 325, 380, 490, 496, 502
BseLI CCNNNNNNNGG 1 cut(s) 416
BseMII CTCAG 1 cut(s) 356
BseSI GKGCMC 1 cut(s) 112
BshNI GGYRCC 1 cut(s) 334
BsiHKAI GWGCWC 1 cut(s) 371
BsiSI CCGG 2 cut(s) 332, 338
BslI CCNNNNNNNGG 1 cut(s) 416
BsmAI GTCTC 1 cut(s) 53
BsmI GAATGC 1 cut(s) 406
Bsp1286I GDGCHC 2 cut(s) 112, 371
Bsp1407I TGTACA 2 cut(s) 66, 78
Bsp143I GATC 1 cut(s) 508
Bsp1720I GCTNAGC 1 cut(s) 405
BspACI CCGC 2 cut(s) 121, 494
BspCNI CTCAG 1 cut(s) 357
BspLI GGNNCC 1 cut(s) 336
BspT107I GGYRCC 1 cut(s) 334
BsrGI TGTACA 2 cut(s) 66, 78
BssMI GATC 1 cut(s) 508
BssNI GRCGYC 1 cut(s) 426
Bst4CI ACNGT 4 cut(s) 34, 64, 204, 458
BstACI GRCGYC 1 cut(s) 426
BstAUI TGTACA 2 cut(s) 66, 78
BstDEI CTNAG 3 cut(s) 307, 365, 405
BstF5I GGATG 6 cut(s) 117, 325, 380, 490, 496, 502
BstKTI GATC 1 cut(s) 511
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 1 cut(s) 508
BstNSI RCATGY 2 cut(s) 184, 347
BstSLI GKGCMC 1 cut(s) 112
BstV2I GAAGAC 1 cut(s) 438
BtgZI GCGATG 3 cut(s) 55, 429, 435
BtsCI GGATG 6 cut(s) 117, 325, 380, 490, 496, 502
BtsIMutI CAGTG 2 cut(s) 463, 518
Csp6I GTAC 2 cut(s) 67, 79
CviAII CATG 4 cut(s) 76, 181, 344, 537
CviJI RGCY 3 cut(s) 153, 301, 409
CviKI_1 RGCY 3 cut(s) 153, 301, 409
CviQI GTAC 2 cut(s) 67, 79
DdeI CTNAG 3 cut(s) 307, 365, 405
DpnI GATC 1 cut(s) 510
DpnII GATC 1 cut(s) 508
Eco57I CTGAAG 2 cut(s) 26, 248
FaeI CATG 4 cut(s) 79, 184, 347, 540
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FatI CATG 4 cut(s) 75, 180, 343, 536
FbaI TGATCA 1 cut(s) 508
FblI GTMKAC 1 cut(s) 259
FokI GGATG 6 cut(s) 104, 332, 367, 497, 503, 509
FspBI CTAG 1 cut(s) 302
HapII CCGG 2 cut(s) 332, 338
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 4 cut(s) 79, 184, 347, 540
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HinfI GANTC 2 cut(s) 361, 533
HpaI GTTAAC 1 cut(s) 37
HpaII CCGG 2 cut(s) 332, 338
HphI GGTGA 3 cut(s) 409, 503, 518
Hpy166II GTNNAC 3 cut(s) 37, 67, 260
Hpy188I TCNGA 5 cut(s) 47, 267, 366, 385, 529
Hpy8I GTNNAC 3 cut(s) 37, 67, 260
Hpy99I CGWCG 1 cut(s) 431
HpyAV CCTTC 3 cut(s) 52, 338, 524
HpyCH4III ACNGT 4 cut(s) 34, 64, 204, 458
HpyCH4IV ACGT 2 cut(s) 354, 426
HpyCH4V TGCA 1 cut(s) 92
HpyF3I CTNAG 3 cut(s) 307, 365, 405
HpySE526I ACGT 2 cut(s) 354, 426
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 4 cut(s) 79, 184, 347, 540
Ksp22I TGATCA 1 cut(s) 508
KspAI GTTAAC 1 cut(s) 37
Kzo9I GATC 1 cut(s) 508
LmnI GCTCC 1 cut(s) 340
LpnPI CCDG 3 cut(s) 98, 345, 351
LweI GCATC 2 cut(s) 481, 487
MaeI CTAG 1 cut(s) 302
MaeII ACGT 2 cut(s) 354, 426
MaeIII GTNAC 2 cut(s) 346, 376
MalI GATC 1 cut(s) 510
MboI GATC 1 cut(s) 508
MboII GAAGA 2 cut(s) 154, 443
MhlI GDGCHC 2 cut(s) 112, 371
MluCI AATT 1 cut(s) 137
MnlI CCTC 1 cut(s) 218
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 1 cut(s) 36
MslI CAYNNNNRTG 1 cut(s) 74
MspI CCGG 2 cut(s) 332, 338
Mva1269I GAATGC 1 cut(s) 406
NdeII GATC 1 cut(s) 508
NlaIII CATG 4 cut(s) 79, 184, 347, 540
NlaIV GGNNCC 1 cut(s) 336
NmuCI GTSAC 1 cut(s) 346
NspI RCATGY 2 cut(s) 184, 347
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PctI GAATGC 1 cut(s) 406
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 2 cut(s) 361, 533
Psp1406I AACGTT 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 336
PsrI GAACNNNNNNTAC 2 cut(s) 188, 220
RsaI GTAC 2 cut(s) 68, 80
RsaNI GTAC 2 cut(s) 67, 79
RseI CAYNNNNRTG 1 cut(s) 74
SaqAI TTAA 1 cut(s) 36
Sau3AI GATC 1 cut(s) 508
SduI GDGCHC 2 cut(s) 112, 371
SetI ASST 9 cut(s) 44, 117, 155, 247, 303, 330, 357, 429, 516
SfaNI GCATC 2 cut(s) 481, 487
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 1 cut(s) 137
SsiI CCGC 2 cut(s) 121, 494
SspMI CTAG 1 cut(s) 302
TaaI ACNGT 4 cut(s) 34, 64, 204, 458
TaiI ACGT 2 cut(s) 357, 429
TaqI TCGA 1 cut(s) 429
TasI AATT 1 cut(s) 137
TatI WGTACW 2 cut(s) 66, 78
TfiI GAWTC 2 cut(s) 361, 533
Tru1I TTAA 1 cut(s) 36
Tru9I TTAA 1 cut(s) 36
TscAI CASTG 2 cut(s) 463, 525
TseFI GTSAC 1 cut(s) 346
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 3 cut(s) 336, 380, 546
TspRI CASTG 2 cut(s) 463, 525
XapI RAATTY 1 cut(s) 137
XceI RCATGY 2 cut(s) 184, 347
XmiI GTMKAC 1 cut(s) 259
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 1 cut(s) 302
ZraI GACGTC 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.