Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0002542

Physical Location & Seq

Genomic Coordinates Reverse (-)

460358 .. 464029

Gene Structure 3672 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ28235

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3165 bp

ATGAACATTGCTCGGCTCCTAGAGGAGGGGTGGAACGGCCGGCGGCCGGAGGGCCTGGCGGTGGTAGAGGCAGCCTGGCATCAGAGGGTCAGGCTTCCCTCACTTGATCGGGTTTGGGCTTGGGTCGTTGGGCCTGGGGTCAAACTCGAACTGAGGGGTGCTTGGGCTTTGGGGCCTAAGTTTGAGTCCAAGCTTGGGCTGTGGGAGTCTAATGCAGAGGCATTGAAAAAGAAGAGCAAGACCAACCTGATGCCGAGATTGTTGGCTCCTGAAGAGGCTGAGGACGGTGCTGGCTCCAACCCTAAGGGGGACTTGGAAGTAACTATAACCCCTAATCCTACACCACCTGAAGGGGTTACCGCCAATGTATATTCCGACAACCCCAATGACAGTGAATATCAGCTCCAGTTTGCCACCATTTCCAACATCATAAAGACAAAGGATTTAACATCCTTACATGACTTTGGAGGTGCCCAAGCATTTGCAGAGGCTCTCGGTAGTGATTTGGAGACTGGGATCCCCACCAACGATGAAGCTACTTGTTGTCGGCTCAAATCCACCACAAATTCCACTACTCCTGAACAGGCTGCTGCAAGAGGCTTCATTCAGCTAGCCAAAATATATTGCAACAGTTACATGATTCTCTTGCTTTTTGTGGCTGCTGTGCTCTCACTTTGCTTTGGAATCGTGGAGGAAGGCCCGATGACTGGTTGGTTTGAAGGGGCCATCATACTTGATGCCATTATCATGCTCGTGGTTCTTCCTTCCATACGTGATTATTGGCGGGAGTCTAAAGGGTTGTCCGGGAGGCAAAAACTGTTGGAGAACACAGATGAAGTGGAGGTTGAGGTCTTAAGAGGCGGAAATGAGGAGAAGATCTCCTCATCAGATATTTTGTTTGGTGACATCGTGTGGTTGAAGAGAGGACATCAGGTTCCTGCTGATGGTTTATTCATATCAGGTGGTTTCGTAGTCTTGGATGATCAATTAGGGGCAGTAGTTGATGCCGATAACCCTTTTCTCTTCTATGGAGCGGAAGTGATAGATGGCAATGGTCGCATGCTTGTGACATCTGTGGGTGTAAACACAGTGTGGGGTGACATGATGAGCAGCATAAACCATTTGAAGAAGACACCATTAGAGGCACAACTTGACAGGGTGAACACTTGTATGCAGATCATTGGTCTCCTAATCTCTATCCTGATCCTTGTGGTGTTGTTCTTGCGTTTTGAACTCAAAAAAGAACTGCACACCGGGGCATCCATGGATCTAAGGGGAGAGCCAACTAAAATGAACATGTTTATGGATGCCATCAAGAAAATTGTCATGAAACCAAGCGGAAAGATTAGTATCTTGACAACTTCTCTTACGGTGATGCTGGTTGGGATAGTAGAAGGGTTACCTTTCTGTATCAACCTTGCCATCATTTACTGGAACAGGAAGGCTTTGAATGGGATGGCCTCTCTTCAACATCTGTTGGCCTGTGTCACCATGGCTTCTGTTACATCTATCTGCACAGACAAAAACGGTGGGCTAACATTGGATTCAATGGAAGTTGACATGTGCTGGATTGGCGAACAAGTTATGGCTGAAAATTCTGTTATAGCCTCAAATGTCCTCGAAGCTTTTCGTGATGGAATCAGCATGCTCCTTCTCTCGCAGCCTCCTCCTTATACCTCAATAGAAGACCCTGTCATTTCCTGGGCGGCACTGAAGTTGGGCATGGAATTGGAAATTGTGAGGCAGAGTAATACCATTGTCGAGGATAAGGAATTGACCACTGATCAGGAAGGAAGAGGAGCAATGATGCGGAAGAACAGAGACAGTGAAAGAGAAAGAATGTACTTGCATTGCAGAGGACCTGCTACAAGTATTTTGCCCATTTGTTCACACTACCATGACTGTAAAGGGATGATCAACGTCATCGAAGAACGAAACAGGGTGACTTTTCATAGAAATGTCGAGGATATGCAGTCCAAGCATCTTAAAACCATGGCATTTGCGTACAAGGAAATTGATGTCCCAACAATGGAAGAAAGAGATCTGATATTGCTGGGACTGGTTGGTCTGAAGTCATGTTTGGAAAAGATGAGGGAATCAATCAGAGCCATAAGAAACGCTGGAGTCAATATCATACTGGTTTCCGGAGATGATGTGCAGACACTAGTGAGCATAGCTGAGCATTTCAGAATTCAGGGGGTGCCGATTTCAAATGAAATGGTGACCACAGGTGAAAAGTTCAGGAATTGCAGTGATGAAGACAGGATGAAGATGGTAAGTGAAATTTCAGTGATGGGAAACTCACTCCCCTCCGACAAGCTTCTTCTGGTTAAGTGTTTGAAGCAAAAAGGGGAAACAGTAGCAGTCATTGGAGTTAAAACGAATGAGGCTCCTGCACTGAAAGAAGCTGATGTTGGACTTACAATGGGAATATGGACCACCAAAATGGCTAAAGCGAGTTCAGACATCATCCTAAGGGTTGCAGATATCAGTTGCCTGGTAAGTATCCTCAGGCATGGAAGATGTGCTTATTACAACATTCAAAAGTACATCCAACTTGAGCTTACCATAGTAGCCGCAGGGCTTCTAGTAACATTTATCATAGCAGCATCTACAGGAAATATGCCCATAACAAGTATCCAATTGTTCTGGGTAAATTTGGTAGTGCCAATGCTGGGCGGCCTGGCTCTATTGACAGAGCTCCCAAGAAAGAAGTTAATGGAGAAATCACAATCTGGACAAACCAAAGCAATTATTACCATGGCCATGTGCAGAAATATAATAATCCAAGCTTCATATCAAGCTGTTACTATAGTCACCTTCCATTTCAAAGGACAAGCAATCTTGGGTGTTGATGAAAAGGTCCGCAAGACTATCATCTTCAATAGTTTTGTCATCTGCCAGATTTTTAACAAGTTCAGTGCGAGAGAGCTGGTAAAGAAAAATGTGTTTAAGGCGATCCACAAGAACCAATGGTTTTGGGTGGCTTTGGCTGCAATCCTAGTGCTACAGCTGAGTTTTATTGAGATTTCACATGTGGTTGCTGGTGATGCAAGGCTGAGCTTGGTGCACTGGGGTTTCTGCCTTCTTATTGGGATTGTTTCGCTAGGGATGGATATAGCTGCAAAGGGCATATATGATTACATATCATCTTTCGTCGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase Hydrolase

Protein Analysis

1054

Amino Acids

116.38

Weight (kDa)

5.98

Isoelectric Point (pI)

30.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	280 - 372	1.8e-08	P-type ATPase actuator domain
Cation_ATPase	PF13246	570 - 691	1.9e-09	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	687 - 804	4.3e-06	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	875 - 1043	2.2e-32	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002542)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g00630 FvH4_3g13880
prunus_persica	Prupe.4G007300_v2.0.a1 Prupe.4G007300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0000841 RchiOBHm_Chr5g0000871
rosa_laevigata	RLG00000030901 RLG00000030902 RLG00000030903 RLG00000032693 RLG00000032697
rosa_multiflora	Rmu_co8361431.1_g000001 Rmu_sc0000255.1_g000013 Rmu_sc0000255.1_g000017 Rmu_sc0001979.1_g000005 Rmu_sc0002085.1_g000003 Rmu_sc0002085.1_g000004 Rmu_sc0010050.1_g000003 Rmu_sc0010050.1_g000006 Rmu_sc0019378.1_g000001 Rmu_sc0034511.1_g000001
rosa_roxburghii	Rroxscaffold_1G00056260 Rroxscaffold_1G00075400
rosa_rugosa	Rorug04G0387000 Rorug04G0387100 Rorug04G0387200 Rorug05G0076900
rosa_samantha	Rh5AG006900 Rh5AG007000 Rh5AG163100 Rh5BG009000 Rh5BG009100 Rh5BG162300 Rh5CG007600 Rh5CG007700 Rh5CG177100 Rh5DG007600 Rh5DG163600
rosa_wichuraiana	Rw5G000710 Rw5G000730 Rw5G014710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2064
Acc36I	ACCTGC	1 cut(s)	1870
AccB1I	GGYRCC	2 cut(s)	470, 2200
AccBSI	CCGCTC	1 cut(s)	1034
AccIII	TCCGGA	1 cut(s)	2144
AclWI	GGATC	5 cut(s)	511, 524, 1198, 1275, 2953
AcoI	YGGCCR	3 cut(s)	37, 44, 2763
AcsI	RAATTY	5 cut(s)	565, 1594, 2190, 2283, 2656
AcuI	CTGAAG	4 cut(s)	291, 369, 1733, 2090
AdeI	CACNNNGTG	1 cut(s)	1092
AfaI	GTAC	3 cut(s)	1844, 2006, 2549
AflII	CTTAAG	1 cut(s)	853
AflIII	ACRYGT	3 cut(s)	1296, 1560, 3034
AhlI	ACTAGT	1 cut(s)	2164
AjnI	CCWGG	6 cut(s)	54, 74, 133, 1700, 2496, 2682
AjuI	GAANNNNNNNTTGG	4 cut(s)	2063, 2095, 2397, 2429
AloI	GAACNNNNNNTCC	2 cut(s)	918, 950
Alw21I	GWGCWC	3 cut(s)	669, 2703, 3072
Alw26I	GTCTC	3 cut(s)	503, 1190, 1815
Alw44I	GTGCAC	1 cut(s)	3068
AlwI	GGATC	5 cut(s)	511, 524, 1198, 1275, 2953
Aor13HI	TCCGGA	1 cut(s)	2144
ApaLI	GTGCAC	1 cut(s)	3068
ApeKI	GCWGC	9 cut(s)	71, 587, 590, 659, 1110, 1660, 2606, 2993, 3122
ApoI	RAATTY	5 cut(s)	565, 1594, 2190, 2283, 2656
Asp700I	GAANNNNTTC	1 cut(s)	1626
AspS9I	GGNCC	8 cut(s)	52, 131, 173, 698, 723, 1859, 2436, 2863
AsuC2I	CCSGG	2 cut(s)	805, 1255
AsuNHI	GCTAGC	1 cut(s)	610
AvaII	GGWCC	3 cut(s)	1859, 2436, 2863
AxyI	CCTNAGG	3 cut(s)	303, 2474, 2510
BaeGI	GKGCMC	2 cut(s)	475, 3072
BalI	TGGCCA	1 cut(s)	2765
BamHI	GGATCC	1 cut(s)	516
BanI	GGYRCC	2 cut(s)	470, 2200
BanII	GRGCYC	1 cut(s)	2703
BauI	CACGAG	1 cut(s)	752
BbsI	GAAGAC	3 cut(s)	1136, 1692, 2265
Bbv12I	GWGCWC	3 cut(s)	669, 2703, 3072
BbvCI	CCTCAGC	1 cut(s)	279
BbvI	GCAGC	9 cut(s)	83, 574, 577, 646, 1122, 1672, 2618, 2980, 3109
BceAI	ACGGC	1 cut(s)	52
BciT130I	CCWGG	6 cut(s)	56, 76, 135, 1702, 2498, 2684
BciVI	GTATCC	2 cut(s)	2516, 2648
BclI	TGATCA	3 cut(s)	982, 1783, 1914
BcnI	CCSGG	2 cut(s)	805, 1255
BcoDI	GTCTC	3 cut(s)	503, 1190, 1815
BcuI	ACTAGT	1 cut(s)	2164
BfaI	CTAG	6 cut(s)	20, 611, 2165, 2588, 3002, 3107
BfmI	CTRYAG	3 cut(s)	2613, 2811, 3008
BfrI	CTTAAG	1 cut(s)	853
BfuAI	ACCTGC	1 cut(s)	1870
BfuI	GTATCC	2 cut(s)	2516, 2648
BglII	AGATCT	2 cut(s)	876, 2041
BlpI	GCTNAGC	2 cut(s)	2178, 3059
Bme1390I	CCNGG	8 cut(s)	56, 76, 135, 805, 1255, 1702, 2498, 2684
Bme18I	GGWCC	3 cut(s)	1859, 2436, 2863
BmgT120I	GGNCC	8 cut(s)	52, 131, 173, 698, 723, 1859, 2436, 2863
BmrFI	CCNGG	8 cut(s)	56, 76, 135, 805, 1255, 1702, 2498, 2684
BmrI	ACTGGG	2 cut(s)	522, 3082
BmtI	GCTAGC	1 cut(s)	614
BmuI	ACTGGG	2 cut(s)	522, 3082
BpiI	GAAGAC	3 cut(s)	1136, 1692, 2265
BplI	GAGNNNNNCTC	2 cut(s)	863, 895
BpmI	CTGGAG	2 cut(s)	389, 2142
Bpu10I	CCTNAGC	1 cut(s)	279
Bpu1102I	GCTNAGC	2 cut(s)	2178, 3059
BpuEI	CTTGAG	1 cut(s)	2579
BpuMI	CCSGG	2 cut(s)	805, 1255
BsaAI	YACGTR	1 cut(s)	773
BsaBI	GATNNNNATC	1 cut(s)	1349
BsaI	GGTCTC	1 cut(s)	1190
BsaJI	CCNNGG	7 cut(s)	134, 1254, 1263, 1491, 1701, 1992, 2760
BsaWI	WCCGGW	1 cut(s)	2144
BsaXI	ACNNNNNCTCC	4 cut(s)	1023, 1053, 2139, 2169
Bse118I	RCCGGY	1 cut(s)	39
Bse1I	ACTGG	7 cut(s)	406, 517, 712, 1436, 2064, 2142, 3077
Bse21I	CCTNAGG	3 cut(s)	303, 2474, 2510
Bse3DI	GCAATG	4 cut(s)	6, 1057, 1809, 1849
Bse8I	GATNNNNATC	1 cut(s)	1349
BseAI	TCCGGA	1 cut(s)	2144
BseBI	CCWGG	6 cut(s)	56, 76, 135, 1702, 2498, 2684
BseDI	CCNNGG	7 cut(s)	134, 1254, 1263, 1491, 1701, 1992, 2760
BseJI	GATNNNNATC	1 cut(s)	1349
BseMI	GCAATG	4 cut(s)	6, 1057, 1809, 1849
BseMII	CTCAG	6 cut(s)	143, 270, 2169, 2524, 3005, 3050
BseNI	ACTGG	7 cut(s)	406, 517, 712, 1436, 2064, 2142, 3077
BseRI	GAGGAG	5 cut(s)	38, 871, 884, 1656, 1812
BseSI	GKGCMC	2 cut(s)	475, 3072
BseX3I	CGGCCG	2 cut(s)	37, 44
BseXI	GCAGC	9 cut(s)	83, 574, 577, 646, 1122, 1672, 2618, 2980, 3109
BseYI	CCCAGC	2 cut(s)	2053, 2674
BsgI	GTGCAG	5 cut(s)	1232, 1498, 2177, 2379, 2791
Bsh1285I	CGRYCG	2 cut(s)	40, 47
BshNI	GGYRCC	2 cut(s)	470, 2200
BsiEI	CGRYCG	2 cut(s)	40, 47
BsiHKAI	GWGCWC	3 cut(s)	669, 2703, 3072
BsiSI	CCGG	5 cut(s)	40, 47, 804, 1254, 2145
BslFI	GGGAC	3 cut(s)	323, 2006, 2070
BsmAI	GTCTC	3 cut(s)	503, 1190, 1815
BsmFI	GGGAC	3 cut(s)	323, 2006, 2070
Bso31I	GGTCTC	1 cut(s)	1190
Bsp1286I	GDGCHC	4 cut(s)	475, 669, 2703, 3072
Bsp13I	TCCGGA	1 cut(s)	2144
Bsp1720I	GCTNAGC	2 cut(s)	2178, 3059
Bsp19I	CCATGG	4 cut(s)	1263, 1491, 1992, 2760
BspCNI	CTCAG	6 cut(s)	144, 271, 2170, 2523, 3006, 3051
BspEI	TCCGGA	1 cut(s)	2144
BspHI	TCATGA	1 cut(s)	1326
BspMI	ACCTGC	1 cut(s)	1870
BspOI	GCTAGC	1 cut(s)	614
BspPI	GGATC	5 cut(s)	511, 524, 1198, 1275, 2953
BspQI	GCTCTTC	1 cut(s)	227
BspT107I	GGYRCC	2 cut(s)	470, 2200
BspTI	CTTAAG	1 cut(s)	853
BspTNI	GGTCTC	1 cut(s)	1190
BsrBI	CCGCTC	1 cut(s)	1034
BsrDI	GCAATG	4 cut(s)	6, 1057, 1809, 1849
BsrFI	RCCGGY	1 cut(s)	39
BsrI	ACTGG	7 cut(s)	406, 517, 712, 1436, 2064, 2142, 3077
BssAI	RCCGGY	1 cut(s)	39
BssECI	CCNNGG	7 cut(s)	134, 1254, 1263, 1491, 1701, 1992, 2760
BssSI	CACGAG	1 cut(s)	752
BssT1I	CCWWGG	4 cut(s)	1263, 1491, 1992, 2760
Bst2BI	CACGAG	1 cut(s)	752
Bst2UI	CCWGG	6 cut(s)	56, 76, 135, 1702, 2498, 2684
Bst6I	CTCTTC	6 cut(s)	227, 267, 914, 1028, 1470, 1789
BstAFI	CTTAAG	1 cut(s)	853
BstBAI	YACGTR	1 cut(s)	773
BstC8I	GCNNGC	5 cut(s)	41, 292, 612, 1061, 1646
BstDSI	CCRYGG	4 cut(s)	1263, 1491, 1992, 2760
BstEII	GGTNACC	3 cut(s)	355, 1398, 2221
BstENI	CCTNNNNNAGG	1 cut(s)	23
BstMAI	GTCTC	3 cut(s)	503, 1190, 1815
BstMCI	CGRYCG	2 cut(s)	40, 47
BstMWI	GCNNNNNNNGC	5 cut(s)	1056, 1572, 1978, 2993, 3050
BstNI	CCWGG	6 cut(s)	56, 76, 135, 1702, 2498, 2684
BstNSI	RCATGY	5 cut(s)	1063, 1300, 1564, 1648, 3038
BstPI	GGTNACC	3 cut(s)	355, 1398, 2221
BstSCI	CCNGG	8 cut(s)	54, 74, 133, 803, 1253, 1700, 2496, 2682
BstSFI	CTRYAG	3 cut(s)	2613, 2811, 3008
BstSLI	GKGCMC	2 cut(s)	475, 3072
BstV1I	GCAGC	9 cut(s)	83, 574, 577, 646, 1122, 1672, 2618, 2980, 3109
BstV2I	GAAGAC	3 cut(s)	1136, 1692, 2265
BstX2I	RGATCY	4 cut(s)	516, 876, 1267, 2041
BstXI	CCANNNNNNTGG	1 cut(s)	2446
BstYI	RGATCY	4 cut(s)	516, 876, 1267, 2041
BstZI	CGGCCG	2 cut(s)	37, 44
Bsu36I	CCTNAGG	3 cut(s)	303, 2474, 2510
BsuI	GTATCC	2 cut(s)	2516, 2648
BtgI	CCRYGG	4 cut(s)	1263, 1491, 1992, 2760
BtsI	GCAGTG	1 cut(s)	2257
BveI	ACCTGC	1 cut(s)	1870
Cac8I	GCNNGC	5 cut(s)	41, 292, 612, 1061, 1646
CciI	TCATGA	1 cut(s)	1326
Cfr10I	RCCGGY	1 cut(s)	39
Cfr13I	GGNCC	8 cut(s)	52, 131, 173, 698, 723, 1859, 2436, 2863
Csp6I	GTAC	3 cut(s)	1843, 2005, 2548
CspCI	CAANNNNNGTGG	2 cut(s)	1510, 1545
CviQI	GTAC	3 cut(s)	1843, 2005, 2548
DraIII	CACNNNGTG	1 cut(s)	1092
DrdI	GACNNNNNNGTC	1 cut(s)	2064
DseDI	GACNNNNNNGTC	1 cut(s)	2064
EaeI	YGGCCR	3 cut(s)	37, 44, 2763
EagI	CGGCCG	2 cut(s)	37, 44
Eam1104I	CTCTTC	6 cut(s)	227, 267, 914, 1028, 1470, 1789
EarI	CTCTTC	6 cut(s)	227, 267, 914, 1028, 1470, 1789
EciI	GGCGGA	1 cut(s)	876
Ecl136II	GAGCTC	1 cut(s)	2701
EclXI	CGGCCG	2 cut(s)	37, 44
Eco130I	CCWWGG	4 cut(s)	1263, 1491, 1992, 2760
Eco24I	GRGCYC	1 cut(s)	2703
Eco31I	GGTCTC	1 cut(s)	1190
Eco32I	GATATC	1 cut(s)	2488
Eco47I	GGWCC	3 cut(s)	1859, 2436, 2863
Eco52I	CGGCCG	2 cut(s)	37, 44
Eco53kI	GAGCTC	1 cut(s)	2701
Eco57I	CTGAAG	4 cut(s)	291, 369, 1733, 2090
Eco81I	CCTNAGG	3 cut(s)	303, 2474, 2510
Eco91I	GGTNACC	3 cut(s)	355, 1398, 2221
EcoICRI	GAGCTC	1 cut(s)	2701
EcoNI	CCTNNNNNAGG	1 cut(s)	23
EcoO109I	RGGNCCY	3 cut(s)	52, 173, 1859
EcoO65I	GGTNACC	3 cut(s)	355, 1398, 2221
EcoRI	GAATTC	1 cut(s)	2190
EcoRII	CCWGG	6 cut(s)	54, 74, 133, 1700, 2496, 2682
EcoRV	GATATC	1 cut(s)	2488
EcoT14I	CCWWGG	4 cut(s)	1263, 1491, 1992, 2760
EcoT38I	GRGCYC	1 cut(s)	2703
ErhI	CCWWGG	4 cut(s)	1263, 1491, 1992, 2760
FalI	AAGNNNNNCTT	4 cut(s)	296, 328, 2512, 2544
FaqI	GGGAC	3 cut(s)	323, 2006, 2070
FauI	CCCGC	1 cut(s)	777
FbaI	TGATCA	3 cut(s)	982, 1783, 1914
FriOI	GRGCYC	1 cut(s)	2703
FspBI	CTAG	6 cut(s)	20, 611, 2165, 2588, 3002, 3107
GsaI	CCCAGC	2 cut(s)	2057, 2678
GsuI	CTGGAG	2 cut(s)	389, 2142
HapII	CCGG	5 cut(s)	40, 47, 804, 1254, 2145
HincII	GTYRAC	1 cut(s)	1558
HindII	GTYRAC	1 cut(s)	1558
HindIII	AAGCTT	4 cut(s)	191, 1623, 2318, 2790
HinfI	GANTC	9 cut(s)	185, 206, 640, 684, 788, 1544, 1638, 2096, 2124
HpaII	CCGG	5 cut(s)	40, 47, 804, 1254, 2145
Hpy166II	GTNNAC	5 cut(s)	1084, 1162, 1558, 1889, 3070
Hpy188I	TCNGA	9 cut(s)	84, 376, 889, 2046, 2070, 2105, 2189, 2314, 2464
Hpy8I	GTNNAC	5 cut(s)	1084, 1162, 1558, 1889, 3070
Hpy99I	CGWCG	1 cut(s)	3161
HpyCH4IV	ACGT	2 cut(s)	772, 1920
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1056, 1572, 1978, 2993, 3050
HpySE526I	ACGT	2 cut(s)	772, 1920
Kpn2I	TCCGGA	1 cut(s)	2144
KroI	GCCGGC	1 cut(s)	39
KroNI	GCCGGC	1 cut(s)	41
Ksp22I	TGATCA	3 cut(s)	982, 1783, 1914
LguI	GCTCTTC	1 cut(s)	227
LmnI	GCTCC	9 cut(s)	21, 271, 299, 408, 1031, 1653, 1799, 2395, 2706
Lsp1109I	GCAGC	9 cut(s)	83, 574, 577, 646, 1122, 1672, 2618, 2980, 3109
MaeI	CTAG	6 cut(s)	20, 611, 2165, 2588, 3002, 3107
MaeII	ACGT	2 cut(s)	772, 1920
MbiI	CCGCTC	1 cut(s)	1034
MfeI	CAATTG	1 cut(s)	2642
MflI	RGATCY	4 cut(s)	516, 876, 1267, 2041
MhlI	GDGCHC	4 cut(s)	475, 669, 2703, 3072
MlsI	TGGCCA	1 cut(s)	2765
MluNI	TGGCCA	1 cut(s)	2765
MlyI	GAGTC	4 cut(s)	194, 215, 797, 2133
MmeI	TCCRAC	7 cut(s)	321, 399, 447, 801, 2337, 2395, 2578
Mox20I	TGGCCA	1 cut(s)	2765
MroI	TCCGGA	1 cut(s)	2144
MroNI	GCCGGC	1 cut(s)	39
MroXI	GAANNNNTTC	1 cut(s)	1626
MscI	TGGCCA	1 cut(s)	2765
MseI	TTAA	8 cut(s)	446, 854, 1986, 2331, 2376, 2717, 2910, 2952
MslI	CAYNNNNRTG	9 cut(s)	746, 752, 1064, 1169, 1301, 1896, 1956, 2444, 2765
Msp20I	TGGCCA	1 cut(s)	2765
MspA1I	CMGCKG	1 cut(s)	3013
MspCI	CTTAAG	1 cut(s)	853
MspI	CCGG	5 cut(s)	40, 47, 804, 1254, 2145
MspR9I	CCNGG	8 cut(s)	56, 76, 135, 805, 1255, 1702, 2498, 2684
MunI	CAATTG	1 cut(s)	2642
MvaI	CCWGG	6 cut(s)	56, 76, 135, 1702, 2498, 2684
MwoI	GCNNNNNNNGC	5 cut(s)	1056, 1572, 1978, 2993, 3050
NaeI	GCCGGC	1 cut(s)	41
NciI	CCSGG	2 cut(s)	805, 1255
NcoI	CCATGG	4 cut(s)	1263, 1491, 1992, 2760
NgoMIV	GCCGGC	1 cut(s)	39
NheI	GCTAGC	1 cut(s)	610
NmeAIII	GCCGAG	1 cut(s)	279
NmuCI	GTSAC	7 cut(s)	902, 1066, 1097, 1486, 1942, 2221, 2815
NspI	RCATGY	5 cut(s)	1063, 1300, 1564, 1648, 3038
PaeI	GCATGC	2 cut(s)	1063, 1648
PagI	TCATGA	1 cut(s)	1326
PciI	ACATGT	3 cut(s)	1296, 1560, 3034
PciSI	GCTCTTC	1 cut(s)	227
PdiI	GCCGGC	1 cut(s)	41
PdmI	GAANNNNTTC	1 cut(s)	1626
PfeI	GAWTC	5 cut(s)	640, 684, 1544, 1638, 2096
PflFI	GACNNNGTC	1 cut(s)	1691
PfoI	TCCNGGA	1 cut(s)	803
PleI	GAGTC	4 cut(s)	193, 214, 796, 2132
PpsI	GAGTC	4 cut(s)	193, 214, 796, 2132
Ppu21I	YACGTR	1 cut(s)	773
PpuMI	RGGWCCY	1 cut(s)	1859
PscI	ACATGT	3 cut(s)	1296, 1560, 3034
Psp124BI	GAGCTC	1 cut(s)	2703
Psp5II	RGGWCCY	1 cut(s)	1859
Psp6I	CCWGG	6 cut(s)	54, 74, 133, 1700, 2496, 2682
PspEI	GGTNACC	3 cut(s)	355, 1398, 2221
PspFI	CCCAGC	2 cut(s)	2053, 2674
PspGI	CCWGG	6 cut(s)	54, 74, 133, 1700, 2496, 2682
PspPI	GGNCC	8 cut(s)	52, 131, 173, 698, 723, 1859, 2436, 2863
PspPPI	RGGWCCY	1 cut(s)	1859
PsuI	RGATCY	4 cut(s)	516, 876, 1267, 2041
PsyI	GACNNNGTC	1 cut(s)	1691
PvuII	CAGCTG	1 cut(s)	3013
RsaI	GTAC	3 cut(s)	1844, 2006, 2549
RsaNI	GTAC	3 cut(s)	1843, 2005, 2548
RseI	CAYNNNNRTG	9 cut(s)	746, 752, 1064, 1169, 1301, 1896, 1956, 2444, 2765
SacI	GAGCTC	1 cut(s)	2703
SapI	GCTCTTC	1 cut(s)	227
SaqAI	TTAA	8 cut(s)	446, 854, 1986, 2331, 2376, 2717, 2910, 2952
Sau96I	GGNCC	8 cut(s)	52, 131, 173, 698, 723, 1859, 2436, 2863
SchI	GAGTC	4 cut(s)	194, 215, 797, 2133
ScrFI	CCNGG	8 cut(s)	56, 76, 135, 805, 1255, 1702, 2498, 2684
SduI	GDGCHC	4 cut(s)	475, 669, 2703, 3072
SfcI	CTRYAG	3 cut(s)	2613, 2811, 3008
SinI	GGWCC	3 cut(s)	1859, 2436, 2863
SmiMI	CAYNNNNRTG	9 cut(s)	746, 752, 1064, 1169, 1301, 1896, 1956, 2444, 2765
SmlI	CTYRAG	2 cut(s)	853, 2558
SmoI	CTYRAG	2 cut(s)	853, 2558
SpeI	ACTAGT	1 cut(s)	2164
SphI	GCATGC	2 cut(s)	1063, 1648
SspMI	CTAG	6 cut(s)	20, 611, 2165, 2588, 3002, 3107
SstI	GAGCTC	1 cut(s)	2703
StyD4I	CCNGG	8 cut(s)	54, 74, 133, 803, 1253, 1700, 2496, 2682
StyI	CCWWGG	4 cut(s)	1263, 1491, 1992, 2760
TaiI	ACGT	2 cut(s)	775, 1923
TaqI	TCGA	6 cut(s)	147, 1620, 1761, 1926, 1962, 3159
TatI	WGTACW	2 cut(s)	1842, 2547
TauI	GCSGC	4 cut(s)	46, 1709, 2579, 2682
TfiI	GAWTC	5 cut(s)	640, 684, 1544, 1638, 2096
Tru1I	TTAA	8 cut(s)	446, 854, 1986, 2331, 2376, 2717, 2910, 2952
Tru9I	TTAA	8 cut(s)	446, 854, 1986, 2331, 2376, 2717, 2910, 2952
TseFI	GTSAC	7 cut(s)	902, 1066, 1097, 1486, 1942, 2221, 2815
TseI	GCWGC	9 cut(s)	71, 587, 590, 659, 1110, 1660, 2606, 2993, 3122
Tsp45I	GTSAC	7 cut(s)	902, 1066, 1097, 1486, 1942, 2221, 2815
Tth111I	GACNNNGTC	1 cut(s)	1691
Vha464I	CTTAAG	1 cut(s)	853
VneI	GTGCAC	1 cut(s)	3068
VpaK11BI	GGWCC	3 cut(s)	1859, 2436, 2863
XagI	CCTNNNNNAGG	1 cut(s)	23
XapI	RAATTY	5 cut(s)	565, 1594, 2190, 2283, 2656
XceI	RCATGY	5 cut(s)	1063, 1300, 1564, 1648, 3038
XmnI	GAANNNNTTC	1 cut(s)	1626
XspI	CTAG	6 cut(s)	20, 611, 2165, 2588, 3002, 3107

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.