Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0002542

Physical Location & Seq

Genomic Coordinates Reverse (-)

478916 .. 485507

Gene Structure 6592 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG006900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2166 bp

ATGGACATGTCAAAGGACAATCCAGGTGGAGTCGATCATAAGGACCTTGAGTCTCAGCAAACAGTGTCAAAAGAGCTTGAACTGGTGCCCATTGATCCTAACCGCCGCTGGCGTACGATAGTGCTGATCCTTCAGGCACGCCATATTTTCATCACCTTAGCAGCTGCTAAAAATCCTGAACTTGGATCTCTTACAGGAGACTTGGAAGTAACTATAACCCCTAATCCTACACCACCTGAAGGGGTTACCGCCAATGTATATTCCGACAACCCCAATGACAGTGAATATCAGCTCCAGTTTGCCACCATTTCCAACATCATAAAGACAAAGGATTTAACATCCTTACATGACTTTGGAGGTGCCCAAGCATTTGCAGAGGCTCTCGGTAGTGATTTGGAGACTGGGATCCCCACCAACGATGAAGCTACTTGTTGTCGGCTCAAATCCACCACAAATTCCACTACTCCTGAACAGGCTGCTGCAAGAGGCTTCATTCAGCTAGCCAAAATATATTGCAACAGTTACATGATTCTCTTGCTTTTTGTGGCTGCTGTGCTCTCACTTTGCTTTGGAATCGTGGAGGAAGGCCCGATGACTGGTTGGTTTGAAGGGGCCATCATACTTGATGCCATTATCATGCTCGTGGTTCTTCCTTCCATACGTGATTATTGGCGGGAGTCTAAAGGGTTGTCCGGGAGGCAAAAACTGTTGGAGAACACAGATGAAGTGGAGGTTGAGGTCTTAAGAGGCGGAAATGAGGAGAAGATCTCCTCATCAGATATTTTGTTTGGTGACATCGTGTGGTTGAAGAGAGGACATCAGGTTCCTGCTGATGGTTTATTCATATCAGGTGGTTTCGTAGTCTTGGATGATCAATTAGGGGCAGTAGTTGATGCCGATAACCCTTTTCTCTTCTATGGAGCGGAAGTGATAGATGGCAATGGTCGCATGCTTGTGACATCTGTGGGTGTAAACACAGTGTGGGGTGACATGATGAGCAGCATAAACCATTTGAAGAAGACACCATTAGAGGCACAACTTGACAGGGTGAACACTTGTATGCAGATCATTGGTCTCCTAATCTCTATCCTGATCCTTGTGGTGTTGTTCTTGCGTTTTGAACTCAAAAAAGAACTGCACACCGGGGCATCCATGGATCTAAGGGGAGAGCCAACTAAAATGAACATGTTTATGGATGCCATCAAGAAAATTGTCATGAAACCAAGCGGAAAGATTAGTATCTTGACAACTTCTCTTACGGTGATGCTGGTTGGGATAGTAGAAGGGTTACCTTTCTGTATCAACCTTGCCATCATTTACTGGAACAGGAAGGCTTTGAATGGGATGGCCTCTCTTCAACATCTGTTGGCCTGTGTCACCATGGCTTCTGTTACATCTATCTGCACAGACAAAAACGGTGGGCTAACATTGGATTCAATGGAAGTTGACATGTGCTGGATTGGCGAACAAGTTATGGCTGAAAATTCTGTTATAGCCTCAAATGTCCTCGAAGCTTTTCGTGATGGAATCAGCATGCTCCTTCTCTCGCAGCCTCCTCCTTATACCTCAATAGAAGACCCTGTCATTTCCTGGGCGGCACTGAAGTTGGGCATGGAATTGGAAATTGTGAGGCAGAGTAATACCATTGTCGAGGATAAGGAATTGACCACTGATCAGGAAGGAAGAGGAGCAATGATGCGGAAGAACAGAGACAGTGAAAGAGAAAGAATGTACTTGCATTGCAGAGGACCTGCTACAAGTATTTTGCCCATTTGTTCACACTACCATGACTGTAAAGGGATGATCAACGTCATCGAAGAACGAAACAGGGTGACTTTTCATAGAAATGTCGAGGATATGCAGTCCAAGCATCTTAAAACCATGGCATTTGCGTACAAGGAAATTGATGTCCCAACAATGGAAGAAAGAGATCTGATATTGCTGGGACTGGTTGGTCTGAAGTCATGTTTGGAAAAGATGAGGGAATCAATCAGAGCCATAAGAAACGCTGGAGTCAATATCATACTGGTTTCCGGAGATGATGTGCAGACACTAGTGAGCATAGCTGAGCATTTCAGAATTCAGGGGGTGCCGATTTCAAATGAAATGGTGACCACAGGTGAAAAGTTCAGGAATTGCAGTGATGAAGACAGGATGAAGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase Hydrolase

Protein Analysis

721

Amino Acids

79.82

Weight (kDa)

5.04

Isoelectric Point (pI)

33.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	243 - 335	1.1e-08	P-type ATPase actuator domain
Cation_ATPase	PF13246	533 - 654	1.1e-09	P-type ATPase, cytoplasmic domain N

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002542)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g00630 FvH4_3g13880
prunus_persica	Prupe.4G007300_v2.0.a1 Prupe.4G007300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0000841 RchiOBHm_Chr5g0000871
rosa_laevigata	RLG00000030901 RLG00000030902 RLG00000030903 RLG00000032693 RLG00000032697
rosa_multiflora	Rmu_co8361431.1_g000001 Rmu_sc0000255.1_g000013 Rmu_sc0000255.1_g000017 Rmu_sc0001979.1_g000005 Rmu_sc0002085.1_g000003 Rmu_sc0002085.1_g000004 Rmu_sc0010050.1_g000003 Rmu_sc0010050.1_g000006 Rmu_sc0019378.1_g000001 Rmu_sc0034511.1_g000001
rosa_roxburghii	Rroxscaffold_1G00056260 Rroxscaffold_1G00075400
rosa_rugosa	Rorug04G0387000 Rorug04G0387100 Rorug04G0387200 Rorug05G0076900
rosa_samantha	Rh5AG006900 Rh5AG007000 Rh5AG163100 Rh5BG009000 Rh5BG009100 Rh5BG162300 Rh5CG007600 Rh5CG007700 Rh5CG177100 Rh5DG007600 Rh5DG163600
rosa_wichuraiana	Rw5G000710 Rw5G000730 Rw5G014710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1953
Acc36I	ACCTGC	1 cut(s)	1759
AccB1I	GGYRCC	3 cut(s)	85, 359, 2089
AccBSI	CCGCTC	1 cut(s)	923
AccIII	TCCGGA	1 cut(s)	2033
AciI	CCGC	9 cut(s)	103, 106, 249, 673, 750, 923, 1227, 1595, 1699
AclWI	GGATC	7 cut(s)	89, 121, 193, 400, 413, 1087, 1164
AcsI	RAATTY	3 cut(s)	454, 1483, 2079
AcuI	CTGAAG	4 cut(s)	116, 258, 1622, 1979
AdeI	CACNNNGTG	1 cut(s)	981
AfaI	GTAC	3 cut(s)	115, 1733, 1895
AfiI	CCNNNNNNNGG	5 cut(s)	182, 239, 596, 833, 1918
AflII	CTTAAG	1 cut(s)	742
AflIII	ACRYGT	3 cut(s)	6, 1185, 1449
AgsI	TTSAA	9 cut(s)	80, 608, 808, 1015, 1121, 1339, 1358, 1437, 2100
AhdI	GACNNNNNGTC	1 cut(s)	49
AhlI	ACTAGT	1 cut(s)	2053
AjnI	CCWGG	2 cut(s)	22, 1589
AjuI	GAANNNNNNNTTGG	2 cut(s)	1952, 1984
AloI	GAACNNNNNNTCC	2 cut(s)	807, 839
AluBI	AGCT	7 cut(s)	76, 164, 292, 425, 499, 1514, 2066
AluI	AGCT	7 cut(s)	76, 164, 292, 425, 499, 1514, 2066
Alw21I	GWGCWC	1 cut(s)	558
Alw26I	GTCTC	5 cut(s)	57, 192, 392, 1079, 1704
AlwI	GGATC	7 cut(s)	89, 121, 193, 400, 413, 1087, 1164
Aor13HI	TCCGGA	1 cut(s)	2033
AoxI	GGCC	4 cut(s)	586, 612, 1347, 1368
ApeKI	GCWGC	7 cut(s)	161, 164, 476, 479, 548, 999, 1549
ApoI	RAATTY	3 cut(s)	454, 1483, 2079
Asp700I	GAANNNNTTC	1 cut(s)	1515
AspS9I	GGNCC	4 cut(s)	43, 587, 612, 1748
AsuC2I	CCSGG	2 cut(s)	694, 1144
AsuHPI	GGTGA	9 cut(s)	145, 803, 998, 1060, 1273, 1369, 1843, 2122, 2132
AsuNHI	GCTAGC	1 cut(s)	499
AvaII	GGWCC	2 cut(s)	43, 1748
BaeGI	GKGCMC	2 cut(s)	90, 364
BamHI	GGATCC	1 cut(s)	405
BanI	GGYRCC	3 cut(s)	85, 359, 2089
BauI	CACGAG	1 cut(s)	641
BbsI	GAAGAC	3 cut(s)	1025, 1581, 2154
Bbv12I	GWGCWC	1 cut(s)	558
BbvI	GCAGC	7 cut(s)	151, 173, 463, 466, 535, 1011, 1561
BccI	CCATC	7 cut(s)	623, 827, 929, 1208, 1319, 1339, 1517
BciT130I	CCWGG	2 cut(s)	24, 1591
BclI	TGATCA	3 cut(s)	871, 1672, 1803
BcnI	CCSGG	2 cut(s)	694, 1144
BcoDI	GTCTC	5 cut(s)	57, 192, 392, 1079, 1704
BcuI	ACTAGT	1 cut(s)	2053
BfaI	CTAG	2 cut(s)	500, 2054
BfrI	CTTAAG	1 cut(s)	742
BfuAI	ACCTGC	1 cut(s)	1759
BglII	AGATCT	2 cut(s)	765, 1930
BisI	GCNGC	9 cut(s)	106, 162, 165, 477, 480, 549, 1000, 1550, 1596
BlpI	GCTNAGC	1 cut(s)	2067
BlsI	GCNGC	9 cut(s)	107, 163, 166, 478, 481, 550, 1001, 1551, 1597
Bme1390I	CCNGG	4 cut(s)	24, 694, 1144, 1591
Bme18I	GGWCC	2 cut(s)	43, 1748
BmeRI	GACNNNNNGTC	1 cut(s)	49
BmgT120I	GGNCC	4 cut(s)	43, 587, 612, 1748
BmiI	GGNNCC	6 cut(s)	87, 361, 407, 613, 825, 2091
BmrFI	CCNGG	4 cut(s)	24, 694, 1144, 1591
BmrI	ACTGGG	1 cut(s)	411
BmsI	GCATC	7 cut(s)	616, 883, 1157, 1186, 1254, 1686, 1879
BmtI	GCTAGC	1 cut(s)	503
BmuI	ACTGGG	1 cut(s)	411
BpiI	GAAGAC	3 cut(s)	1025, 1581, 2154
BplI	GAGNNNNNCTC	2 cut(s)	752, 784
BpmI	CTGGAG	2 cut(s)	278, 2031
Bpu10I	CCTNAGC	1 cut(s)	157
Bpu1102I	GCTNAGC	1 cut(s)	2067
BpuEI	CTTGAG	1 cut(s)	68
BpuMI	CCSGG	2 cut(s)	694, 1144
BsaAI	YACGTR	1 cut(s)	662
BsaBI	GATNNNNATC	1 cut(s)	1238
BsaI	GGTCTC	1 cut(s)	1079
BsaJI	CCNNGG	5 cut(s)	1143, 1152, 1380, 1590, 1881
BsaWI	WCCGGW	1 cut(s)	2033
BsaXI	ACNNNNNCTCC	4 cut(s)	912, 942, 2028, 2058
Bsc4I	CCNNNNNNNGG	5 cut(s)	182, 239, 596, 833, 1918
Bse1I	ACTGG	7 cut(s)	87, 295, 406, 601, 1325, 1953, 2031
Bse3DI	GCAATG	3 cut(s)	946, 1698, 1738
Bse8I	GATNNNNATC	1 cut(s)	1238
BseAI	TCCGGA	1 cut(s)	2033
BseBI	CCWGG	2 cut(s)	24, 1591
BseDI	CCNNGG	5 cut(s)	1143, 1152, 1380, 1590, 1881
BseGI	GGATG	7 cut(s)	338, 874, 1148, 1201, 1350, 1806, 2160
BseJI	GATNNNNATC	1 cut(s)	1238
BseLI	CCNNNNNNNGG	5 cut(s)	182, 239, 596, 833, 1918
BseMI	GCAATG	3 cut(s)	946, 1698, 1738
BseMII	CTCAG	2 cut(s)	68, 2058
BseNI	ACTGG	7 cut(s)	87, 295, 406, 601, 1325, 1953, 2031
BseRI	GAGGAG	4 cut(s)	760, 773, 1545, 1701
BseSI	GKGCMC	2 cut(s)	90, 364
BseXI	GCAGC	7 cut(s)	151, 173, 463, 466, 535, 1011, 1561
BseYI	CCCAGC	1 cut(s)	1942
BsgI	GTGCAG	3 cut(s)	1121, 1387, 2066
BshFI	GGCC	4 cut(s)	588, 614, 1349, 1370
BshNI	GGYRCC	3 cut(s)	85, 359, 2089
BsiHKAI	GWGCWC	1 cut(s)	558
BsiSI	CCGG	3 cut(s)	693, 1143, 2034
BsiWI	CGTACG	1 cut(s)	113
BslFI	GGGAC	2 cut(s)	1895, 1959
BslI	CCNNNNNNNGG	5 cut(s)	182, 239, 596, 833, 1918
BsmAI	GTCTC	5 cut(s)	57, 192, 392, 1079, 1704
BsmFI	GGGAC	2 cut(s)	1895, 1959
BsnI	GGCC	4 cut(s)	588, 614, 1349, 1370
Bso31I	GGTCTC	1 cut(s)	1079
Bsp1286I	GDGCHC	3 cut(s)	90, 364, 558
Bsp13I	TCCGGA	1 cut(s)	2033
Bsp1720I	GCTNAGC	1 cut(s)	2067
Bsp19I	CCATGG	3 cut(s)	1152, 1380, 1881
BspACI	CCGC	9 cut(s)	103, 106, 249, 673, 750, 923, 1227, 1595, 1699
BspANI	GGCC	4 cut(s)	588, 614, 1349, 1370
BspCNI	CTCAG	2 cut(s)	67, 2059
BspEI	TCCGGA	1 cut(s)	2033
BspHI	TCATGA	1 cut(s)	1215
BspLI	GGNNCC	6 cut(s)	87, 361, 407, 613, 825, 2091
BspMI	ACCTGC	1 cut(s)	1759
BspOI	GCTAGC	1 cut(s)	503
BspPI	GGATC	7 cut(s)	89, 121, 193, 400, 413, 1087, 1164
BspT107I	GGYRCC	3 cut(s)	85, 359, 2089
BspTI	CTTAAG	1 cut(s)	742
BspTNI	GGTCTC	1 cut(s)	1079
BsrBI	CCGCTC	1 cut(s)	923
BsrDI	GCAATG	3 cut(s)	946, 1698, 1738
BsrI	ACTGG	7 cut(s)	87, 295, 406, 601, 1325, 1953, 2031
BssECI	CCNNGG	5 cut(s)	1143, 1152, 1380, 1590, 1881
BssSI	CACGAG	1 cut(s)	641
BssT1I	CCWWGG	3 cut(s)	1152, 1380, 1881
Bst2BI	CACGAG	1 cut(s)	641
Bst2UI	CCWGG	2 cut(s)	24, 1591
Bst4CI	ACNGT	9 cut(s)	64, 281, 521, 708, 979, 1261, 1418, 1715, 1793
Bst6I	CTCTTC	4 cut(s)	803, 917, 1359, 1678
BstAFI	CTTAAG	1 cut(s)	742
BstBAI	YACGTR	1 cut(s)	662
BstC8I	GCNNGC	5 cut(s)	110, 139, 501, 950, 1535
BstDEI	CTNAG	4 cut(s)	54, 157, 1160, 2067
BstDSI	CCRYGG	3 cut(s)	1152, 1380, 1881
BstEII	GGTNACC	3 cut(s)	244, 1287, 2110
BstF5I	GGATG	7 cut(s)	338, 874, 1148, 1201, 1350, 1806, 2160
BstMAI	GTCTC	5 cut(s)	57, 192, 392, 1079, 1704
BstMWI	GCNNNNNNNGC	3 cut(s)	945, 1461, 1867
BstNI	CCWGG	2 cut(s)	24, 1591
BstNSI	RCATGY	5 cut(s)	10, 952, 1189, 1453, 1537
BstPI	GGTNACC	3 cut(s)	244, 1287, 2110
BstSCI	CCNGG	4 cut(s)	22, 692, 1142, 1589
BstSLI	GKGCMC	2 cut(s)	90, 364
BstV1I	GCAGC	7 cut(s)	151, 173, 463, 466, 535, 1011, 1561
BstV2I	GAAGAC	3 cut(s)	1025, 1581, 2154
BstX2I	RGATCY	5 cut(s)	185, 405, 765, 1156, 1930
BstYI	RGATCY	5 cut(s)	185, 405, 765, 1156, 1930
BsuRI	GGCC	4 cut(s)	588, 614, 1349, 1370
BtgI	CCRYGG	3 cut(s)	1152, 1380, 1881
BtsCI	GGATG	7 cut(s)	338, 874, 1148, 1201, 1350, 1806, 2160
BtsI	GCAGTG	1 cut(s)	2146
BtsIMutI	CAGTG	7 cut(s)	69, 286, 984, 1598, 1668, 1720, 2146
BveI	ACCTGC	1 cut(s)	1759
Cac8I	GCNNGC	5 cut(s)	110, 139, 501, 950, 1535
CciI	TCATGA	1 cut(s)	1215
Cfr13I	GGNCC	4 cut(s)	43, 587, 612, 1748
Csp6I	GTAC	3 cut(s)	114, 1732, 1894
CspCI	CAANNNNNGTGG	4 cut(s)	7, 42, 1399, 1434
CviQI	GTAC	3 cut(s)	114, 1732, 1894
DdeI	CTNAG	4 cut(s)	54, 157, 1160, 2067
DraIII	CACNNNGTG	1 cut(s)	981
DrdI	GACNNNNNNGTC	1 cut(s)	1953
DriI	GACNNNNNGTC	1 cut(s)	49
DseDI	GACNNNNNNGTC	1 cut(s)	1953
Eam1104I	CTCTTC	4 cut(s)	803, 917, 1359, 1678
Eam1105I	GACNNNNNGTC	1 cut(s)	49
EarI	CTCTTC	4 cut(s)	803, 917, 1359, 1678
EciI	GGCGGA	1 cut(s)	765
Eco130I	CCWWGG	3 cut(s)	1152, 1380, 1881
Eco31I	GGTCTC	1 cut(s)	1079
Eco47I	GGWCC	2 cut(s)	43, 1748
Eco57I	CTGAAG	4 cut(s)	116, 258, 1622, 1979
Eco91I	GGTNACC	3 cut(s)	244, 1287, 2110
EcoO109I	RGGNCCY	2 cut(s)	43, 1748
EcoO65I	GGTNACC	3 cut(s)	244, 1287, 2110
EcoRI	GAATTC	1 cut(s)	2079
EcoRII	CCWGG	2 cut(s)	22, 1589
EcoT14I	CCWWGG	3 cut(s)	1152, 1380, 1881
ErhI	CCWWGG	3 cut(s)	1152, 1380, 1881
FaqI	GGGAC	2 cut(s)	1895, 1959
FauI	CCCGC	1 cut(s)	666
FbaI	TGATCA	3 cut(s)	871, 1672, 1803
Fnu4HI	GCNGC	9 cut(s)	106, 162, 165, 477, 480, 549, 1000, 1550, 1596
FokI	GGATG	6 cut(s)	325, 881, 1135, 1208, 1357, 1813
Fsp4HI	GCNGC	9 cut(s)	106, 162, 165, 477, 480, 549, 1000, 1550, 1596
FspBI	CTAG	2 cut(s)	500, 2054
GluI	GCNGC	9 cut(s)	106, 162, 165, 477, 480, 549, 1000, 1550, 1596
GsaI	CCCAGC	1 cut(s)	1946
GsuI	CTGGAG	2 cut(s)	278, 2031
HaeIII	GGCC	4 cut(s)	588, 614, 1349, 1370
HapII	CCGG	3 cut(s)	693, 1143, 2034
HincII	GTYRAC	1 cut(s)	1447
HindII	GTYRAC	1 cut(s)	1447
HindIII	AAGCTT	1 cut(s)	1512
HinfI	GANTC	9 cut(s)	30, 50, 529, 573, 677, 1433, 1527, 1985, 2013
HpaII	CCGG	3 cut(s)	693, 1143, 2034
HphI	GGTGA	9 cut(s)	145, 803, 998, 1060, 1273, 1369, 1843, 2122, 2132
Hpy166II	GTNNAC	4 cut(s)	973, 1051, 1447, 1778
Hpy188I	TCNGA	6 cut(s)	265, 778, 1935, 1959, 1994, 2078
Hpy8I	GTNNAC	4 cut(s)	973, 1051, 1447, 1778
HpyAV	CCTTC	9 cut(s)	140, 233, 578, 602, 663, 1277, 1324, 1550, 1673
HpyCH4III	ACNGT	9 cut(s)	64, 281, 521, 708, 979, 1261, 1418, 1715, 1793
HpyCH4IV	ACGT	2 cut(s)	661, 1809
HpyF10VI	GCNNNNNNNGC	3 cut(s)	945, 1461, 1867
HpyF3I	CTNAG	4 cut(s)	54, 157, 1160, 2067
HpySE526I	ACGT	2 cut(s)	661, 1809
Kpn2I	TCCGGA	1 cut(s)	2033
Ksp22I	TGATCA	3 cut(s)	871, 1672, 1803
LmnI	GCTCC	4 cut(s)	297, 920, 1542, 1688
Lsp1109I	GCAGC	7 cut(s)	151, 173, 463, 466, 535, 1011, 1561
LweI	GCATC	7 cut(s)	616, 883, 1157, 1186, 1254, 1686, 1879
MaeI	CTAG	2 cut(s)	500, 2054
MaeII	ACGT	2 cut(s)	661, 1809
MbiI	CCGCTC	1 cut(s)	923
MflI	RGATCY	5 cut(s)	185, 405, 765, 1156, 1930
MhlI	GDGCHC	3 cut(s)	90, 364, 558
MlyI	GAGTC	4 cut(s)	39, 59, 686, 2022
MmeI	TCCRAC	3 cut(s)	288, 336, 690
MroI	TCCGGA	1 cut(s)	2033
MroXI	GAANNNNTTC	1 cut(s)	1515
MseI	TTAA	3 cut(s)	335, 743, 1875
MslI	CAYNNNNRTG	7 cut(s)	635, 641, 953, 1058, 1190, 1785, 1845
MspA1I	CMGCKG	2 cut(s)	108, 164
MspCI	CTTAAG	1 cut(s)	742
MspI	CCGG	3 cut(s)	693, 1143, 2034
MspR9I	CCNGG	4 cut(s)	24, 694, 1144, 1591
MvaI	CCWGG	2 cut(s)	24, 1591
MwoI	GCNNNNNNNGC	3 cut(s)	945, 1461, 1867
NciI	CCSGG	2 cut(s)	694, 1144
NcoI	CCATGG	3 cut(s)	1152, 1380, 1881
NheI	GCTAGC	1 cut(s)	499
NlaIV	GGNNCC	6 cut(s)	87, 361, 407, 613, 825, 2091
NmuCI	GTSAC	6 cut(s)	791, 955, 986, 1375, 1831, 2110
NspI	RCATGY	5 cut(s)	10, 952, 1189, 1453, 1537
PaeI	GCATGC	2 cut(s)	952, 1537
PagI	TCATGA	1 cut(s)	1215
PciI	ACATGT	3 cut(s)	6, 1185, 1449
PdmI	GAANNNNTTC	1 cut(s)	1515
PfeI	GAWTC	5 cut(s)	529, 573, 1433, 1527, 1985
Pfl23II	CGTACG	1 cut(s)	113
PflFI	GACNNNGTC	1 cut(s)	1580
PfoI	TCCNGGA	1 cut(s)	692
PkrI	GCNGC	9 cut(s)	107, 163, 166, 478, 481, 550, 1001, 1551, 1597
PleI	GAGTC	4 cut(s)	38, 58, 685, 2021
PpsI	GAGTC	4 cut(s)	38, 58, 685, 2021
Ppu21I	YACGTR	1 cut(s)	662
PpuMI	RGGWCCY	2 cut(s)	43, 1748
PscI	ACATGT	3 cut(s)	6, 1185, 1449
Psp5II	RGGWCCY	2 cut(s)	43, 1748
Psp6I	CCWGG	2 cut(s)	22, 1589
PspEI	GGTNACC	3 cut(s)	244, 1287, 2110
PspFI	CCCAGC	1 cut(s)	1942
PspGI	CCWGG	2 cut(s)	22, 1589
PspLI	CGTACG	1 cut(s)	113
PspN4I	GGNNCC	6 cut(s)	87, 361, 407, 613, 825, 2091
PspPI	GGNCC	4 cut(s)	43, 587, 612, 1748
PspPPI	RGGWCCY	2 cut(s)	43, 1748
PsuI	RGATCY	5 cut(s)	185, 405, 765, 1156, 1930
PsyI	GACNNNGTC	1 cut(s)	1580
PvuII	CAGCTG	1 cut(s)	164
RsaI	GTAC	3 cut(s)	115, 1733, 1895
RsaNI	GTAC	3 cut(s)	114, 1732, 1894
RseI	CAYNNNNRTG	7 cut(s)	635, 641, 953, 1058, 1190, 1785, 1845
SaqAI	TTAA	3 cut(s)	335, 743, 1875
SatI	GCNGC	9 cut(s)	106, 162, 165, 477, 480, 549, 1000, 1550, 1596
Sau96I	GGNCC	4 cut(s)	43, 587, 612, 1748
SchI	GAGTC	4 cut(s)	39, 59, 686, 2022
ScrFI	CCNGG	4 cut(s)	24, 694, 1144, 1591
SduI	GDGCHC	3 cut(s)	90, 364, 558
SfaNI	GCATC	7 cut(s)	616, 883, 1157, 1186, 1254, 1686, 1879
SinI	GGWCC	2 cut(s)	43, 1748
SmiMI	CAYNNNNRTG	7 cut(s)	635, 641, 953, 1058, 1190, 1785, 1845
SmlI	CTYRAG	2 cut(s)	47, 742
SmoI	CTYRAG	2 cut(s)	47, 742
SpeI	ACTAGT	1 cut(s)	2053
SphI	GCATGC	2 cut(s)	952, 1537
SsiI	CCGC	9 cut(s)	103, 106, 249, 673, 750, 923, 1227, 1595, 1699
SspMI	CTAG	2 cut(s)	500, 2054
StyD4I	CCNGG	4 cut(s)	22, 692, 1142, 1589
StyI	CCWWGG	3 cut(s)	1152, 1380, 1881
TaaI	ACNGT	9 cut(s)	64, 281, 521, 708, 979, 1261, 1418, 1715, 1793
TaiI	ACGT	2 cut(s)	664, 1812
TaqI	TCGA	5 cut(s)	33, 1509, 1650, 1815, 1851
TatI	WGTACW	1 cut(s)	1731
TauI	GCSGC	2 cut(s)	108, 1598
TfiI	GAWTC	5 cut(s)	529, 573, 1433, 1527, 1985
Tru1I	TTAA	3 cut(s)	335, 743, 1875
Tru9I	TTAA	3 cut(s)	335, 743, 1875
TscAI	CASTG	7 cut(s)	69, 286, 984, 1605, 1675, 1720, 2146
TseFI	GTSAC	6 cut(s)	791, 955, 986, 1375, 1831, 2110
TseI	GCWGC	7 cut(s)	161, 164, 476, 479, 548, 999, 1549
Tsp45I	GTSAC	6 cut(s)	791, 955, 986, 1375, 1831, 2110
TspRI	CASTG	7 cut(s)	69, 286, 984, 1605, 1675, 1720, 2146
Tth111I	GACNNNGTC	1 cut(s)	1580
Vha464I	CTTAAG	1 cut(s)	742
VpaK11BI	GGWCC	2 cut(s)	43, 1748
XapI	RAATTY	3 cut(s)	454, 1483, 2079
XceI	RCATGY	5 cut(s)	10, 952, 1189, 1453, 1537
XmnI	GAANNNNTTC	1 cut(s)	1515
XspI	CTAG	2 cut(s)	500, 2054

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.