Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0002542

Physical Location & Seq

Genomic Coordinates Forward (+)

16917557 .. 16925652

Gene Structure 8096 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG162300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3051 bp

ATGGGGAGTTCTGGTGAAGTCGACCTTGAGAATCAACAAACGGTTCCCCGTGTGGCGACTGATCGTGGAGGCGGCTGGCTAACTAGTCTCTTGAATGTAATTCATTCATTTTGCGAGTTTCTGATCATAAAACCTTCTTCTGCTGATGATGAAAGTGAGGAACTGGAACTTACCAGAACTACACACCTTGCATCTGCATCACCTCACGTTTCTAAAGCAGTATCAAGTTTGAGCGTGGAGGATGATGGTACCATCACTAGTACTGCTACCGTTTCTGACCAAGTGAATCTGAACATTGAGAATGATATTACTACCAGGATTGCTAATGAGGCTAAGATCCAGCATGCAAATATTTCCAAGATAGTAAAGGAGAAGGACTTCGCTACCTTACAGAAGTTTGGAGGCATCAAAGGAATTGCAGAGGCACTCAGTACAGATTTACACAGTGGAATCTCAGGAGATGAACAAGATCTTCATGATCGACGCAGTGCAGTAGTCTCAACAACACAGGCTCCAACTCCAAGCTTCTTTAAACTTCTCCGACAATCTTGTTTCGATTGGACCGTTTTCCTTCTCTTCTTGTGTGTCATCCTGTCAACTGTATTTGGTATCCAGCAGGAAGGCCCAAGTAATGGTTGGCTTGAAGGAGCTGTCGGAATGTTTGCGATCATCATACTTGTGGTTGTACCTACAATGCGCAAGTATTTCGCCATGCCGCATCCCAGGAATACTAGTCATCAAATGAAAACTAAGGTCCGCAGAGGAGGAAACCTGCTTCATATCTCCCCATCGGATGTTGTGCCAGGTGACATAGTATGCTTGGAATTGGGATCTCTAGTTCCTGCTGATGGTTTGTTCGTTTCGACTGGTGAATCCTTAGTGTTGGAAGATGGTATGAAATTTCCAACCGTCGATGACAGCAATCCTTTTCTGGTTTATGGTGCAAGGGTGGTAAATGGTAATGGTCGCATGTTGGTTACTTGTGTGGGGATGAAGACAACATTAGGTGAGCTGATGGGCCAAGTTAGATGTACACCTAACCAGGACCAATTACCAGCTCAACTTGATAACTTGAGCACAAGGTTGCAGAATGTTGGGCTCTTGATCACAATTTTCATGACTGTAGTGCTTATCATTCGTTCCGTGGTTCTAAAGGTTAATGAGTACCCTGGCCTCCAAGCTGATCAGTTCAAGGCTAAACCAACTGCAAGCGAGGAGCTAATCAATGTCATTGGCAGATTTTTCATGAAATCAAGTGGGCAGATTAGTGTGTTGGCGACGTATCTGCCTTTATTGATTGTAGGCTTAGCGGAAGGAGTACCTTTTTCAGTTGCACTTGCCATCAATTATTGGACTAAGACCACGCTGTCTGGGAAAGCTAGTGCTCAGAGAATCTTAGCTTGTTTCACCATGGGCTGTGTGTCAAATATTTGCATTGGTGATGGGCTAACTTTGAACCCTGTAATTGAAGTCGACGTGAGCTGTGATGGGGATCAAATAATCGATACAGAGAGTGTTGCTGGTATTAACAGACACGTTCGTGAAGCTTTATACAAAGGAATTAGCACACCACTTTTGATGCCTTCAAGCCCTTGTAGCTCAACAGAACACCGGGTCCTGCTACCGTGGGCCAAATCTAATTTGGGTATGGACAATGAGAATTTATTGCAAAATTGCACCATTGTGGGGAGCGAAAAGTTGAGCACCGATGAGGAGGCTAGTGGAGTTCTGATGAAGGACAATGAAACTGGAGATATGTGCTTTTATTGGAATGGACCTGCAACAACAATATTGCCCATGTGTTCCGAATACTATGACAGCAAGGGGACAACAAAGCTCATGGATGAACAGAAGAAACGTGATTTTAAAGAAATTATGGGGAACATGCAGTCCAAACATCTCAAGACCATTGCATTTGCGTACAAGAAGATTGATGCTCTAATGCCTGAAGAAGGCAACAGCTTAATATTGATAGGACTGCTAGGTGTGAAGTGTTGCACTGAGACAATGGAAGCACTGGAAGTTCTACAAAAAGCAGGAGTGGACATCATCCAGTTCCCAAAAGATAAAGAGTCAGTACTGTCGGAAGGTGAAAGTCCTGTTTATGGAATGGGAAGACCTGCTGACAAGCTCCACAAACTGCAACGTCTGAAGGAAGAGGGCCATGTTGTAGCAATGGTTGGGGCTAGAACAAATGAGATTCCAGCTCTAAAAGAAGCCGATGTGGGGATTGCAATAGGGACTTGCATTAGTGAGATGGCAAGAGAGGGTTCTGATCTCATCATCCCCGATTGCAGTCTCAGTTTCTTGGTCAACATTATTCGGATCGGAAGATGCATTCACTACAACATTCAGAAGTACATCCAAGTTGAGCTCACCATGAATATATCATTGCTTGTGACAATCTCGATCACTACAATGTCCTCGGGACATTGCACAGTTGGGGCAATCGAAGTAGTGTGGGCAAATATGGTTGTGACACTATGTGGGCTTGCGCTACTGGTTGAGCCGCCAACCGAGGAGCAGATGGCAAAGCCGCCGGTAAAACAAACTGAATCTCTCATTACCAAGGCAATGTGGAGAAACATATTACTTCAAGCTTTATATCAGGCTGCCATCTTGGTCACCTTTCAGTCCAAATATAAAGCTCTCCCAGGCATCAACGAGAAGATTATGGAGTCCATAGTTTTCAGCAGTTATGTTCTTTGTCAGGTATTTAATCAAGTCAGCTCAAGGGAGCTAGAGAAGAAAAATGTGTTCAGAGGTATACTTCGAACTCGATGGTTATGGGTGGCTGTGGGGGTTAGTATCACGATGCAGGTGGCTTTTGTTGAGATTGCACACAAGGTTGCTGGCAATGCAAGGTTGAATTGGGCACAATGGGGTGTCTGTCTTTTAATAGCAATGGTTTCCTGGCCAATCGATTTGGCTGGGAAATGCGTATGGGGTGTCATCATGAAGATTAGAATACTTGAATCTGATATTGGATCCACCAGTATGAGGTCCTCTGCTGTCTCAGAATCTGCTTCTAATCGAGAGCTCCTAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase Hydrolase

Protein Analysis

1016

Amino Acids

110.98

Weight (kDa)

5.62

Isoelectric Point (pI)

38.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	247 - 342	6.4e-10	P-type ATPase actuator domain
Cation_ATPase	PF13246	548 - 662	2.9e-07	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	816 - 979	3.1e-34	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002542)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g00630 FvH4_3g13880
prunus_persica	Prupe.4G007300_v2.0.a1 Prupe.4G007300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0000841 RchiOBHm_Chr5g0000871
rosa_laevigata	RLG00000030901 RLG00000030902 RLG00000030903 RLG00000032693 RLG00000032697
rosa_multiflora	Rmu_co8361431.1_g000001 Rmu_sc0000255.1_g000013 Rmu_sc0000255.1_g000017 Rmu_sc0001979.1_g000005 Rmu_sc0002085.1_g000003 Rmu_sc0002085.1_g000004 Rmu_sc0010050.1_g000003 Rmu_sc0010050.1_g000006 Rmu_sc0019378.1_g000001 Rmu_sc0034511.1_g000001
rosa_roxburghii	Rroxscaffold_1G00056260 Rroxscaffold_1G00075400
rosa_rugosa	Rorug04G0387000 Rorug04G0387100 Rorug04G0387200 Rorug05G0076900
rosa_samantha	Rh5AG006900 Rh5AG007000 Rh5AG163100 Rh5BG009000 Rh5BG009100 Rh5BG162300 Rh5CG007600 Rh5CG007700 Rh5CG177100 Rh5DG007600 Rh5DG163600
rosa_wichuraiana	Rw5G000710 Rw5G000730 Rw5G014710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2809
AasI	GACNNNNNNGTC	1 cut(s)	1366
Acc16I	TGCGCA	1 cut(s)	698
Acc36I	ACCTGC	4 cut(s)	780, 1786, 2128, 2809
Acc65I	GGTACC	1 cut(s)	248
AccB1I	GGYRCC	1 cut(s)	248
AccB7I	CCANNNNNTGG	1 cut(s)	632
AccI	GTMKAC	3 cut(s)	21, 1473, 2767
AciI	CCGC	6 cut(s)	72, 716, 757, 1310, 2510, 2537
AclWI	GGATC	6 cut(s)	331, 838, 1500, 2333, 2982, 2995
AcoI	YGGCCR	1 cut(s)	2915
AcsI	RAATTY	2 cut(s)	899, 1660
AcuI	CTGAAG	2 cut(s)	1968, 2171
AdeI	CACNNNGTG	1 cut(s)	2486
AfiI	CCNNNNNNNGG	7 cut(s)	632, 848, 1686, 1952, 2105, 2226, 3000
AflIII	ACRYGT	1 cut(s)	1534
AgsI	TTSAA	9 cut(s)	94, 644, 1192, 1456, 1469, 1587, 2597, 2869, 2975
AhlI	ACTAGT	3 cut(s)	83, 257, 731
AjiI	CACGTC	1 cut(s)	1477
AjnI	CCWGG	7 cut(s)	314, 722, 802, 1041, 1168, 2653, 2912
AjuI	GAANNNNNNNTTGG	2 cut(s)	2967, 2999
AleI	CACNNNNGTG	2 cut(s)	1539, 1682
AloI	GAACNNNNNNTCC	6 cut(s)	822, 854, 1599, 1631, 1874, 1906
Alw21I	GWGCWC	5 cut(s)	1079, 1387, 1706, 2376, 3042
Alw26I	GTCTC	5 cut(s)	92, 502, 1997, 2303, 3019
AlwI	GGATC	6 cut(s)	331, 838, 1500, 2333, 2982, 2995
AlwNI	CAGNNNCTG	1 cut(s)	3023
Ama87I	CYCGRG	1 cut(s)	2425
AoxI	GGCC	6 cut(s)	622, 1018, 1171, 1629, 2161, 2915
ApeKI	GCWGC	1 cut(s)	2612
ApoI	RAATTY	2 cut(s)	899, 1660
Asp700I	GAANNNNTTC	1 cut(s)	377
Asp718I	GGTACC	1 cut(s)	248
AspLEI	GCGC	2 cut(s)	699, 2497
AsuC2I	CCSGG	1 cut(s)	1613
AsuII	TTCGAA	1 cut(s)	2773
AvaI	CYCGRG	1 cut(s)	2425
AvaII	GGWCC	6 cut(s)	561, 754, 1045, 1615, 1775, 3003
BaeGI	GKGCMC	1 cut(s)	2878
BalI	TGGCCA	1 cut(s)	2917
BamHI	GGATCC	1 cut(s)	2987
BanI	GGYRCC	1 cut(s)	248
BanII	GRGCYC	3 cut(s)	1101, 2376, 3042
BbsI	GAAGAC	2 cut(s)	1001, 2122
Bbv12I	GWGCWC	5 cut(s)	1079, 1387, 1706, 2376, 3042
BbvI	GCAGC	1 cut(s)	2599
BcgI	CGANNNNNNTGC	4 cut(s)	688, 722, 1267, 1301
BciT130I	CCWGG	7 cut(s)	316, 724, 804, 1043, 1170, 2655, 2914
BciVI	GTATCC	1 cut(s)	620
BclI	TGATCA	3 cut(s)	123, 1104, 1183
BcnI	CCSGG	1 cut(s)	1613
BcoDI	GTCTC	5 cut(s)	92, 502, 1997, 2303, 3019
BcuI	ACTAGT	3 cut(s)	83, 257, 731
BfmI	CTRYAG	1 cut(s)	1122
BfuAI	ACCTGC	4 cut(s)	780, 1786, 2128, 2809
BfuI	GTATCC	1 cut(s)	620
BglII	AGATCT	1 cut(s)	469
BisI	GCNGC	5 cut(s)	73, 716, 2510, 2537, 2613
BlpI	GCTNAGC	1 cut(s)	1306
BlsI	GCNGC	5 cut(s)	74, 717, 2511, 2538, 2614
BmcAI	AGTACT	2 cut(s)	262, 2079
Bme1390I	CCNGG	8 cut(s)	316, 724, 804, 1043, 1170, 1613, 2655, 2914
Bme18I	GGWCC	6 cut(s)	561, 754, 1045, 1615, 1775, 3003
BmeT110I	CYCGRG	1 cut(s)	2425
BmgBI	CACGTC	1 cut(s)	1477
BmiI	GGNNCC	5 cut(s)	45, 250, 513, 1616, 2989
BmrFI	CCNGG	8 cut(s)	316, 724, 804, 1043, 1170, 1613, 2655, 2914
BmsI	GCATC	9 cut(s)	200, 206, 414, 727, 1569, 1924, 2324, 2667, 2805
BpiI	GAAGAC	2 cut(s)	1001, 2122
BpmI	CTGGAG	1 cut(s)	1770
Bpu1102I	GCTNAGC	1 cut(s)	1306
Bpu14I	TTCGAA	1 cut(s)	2773
BpuEI	CTTGAG	4 cut(s)	47, 1093, 1886, 2716
BpuMI	CCSGG	1 cut(s)	1613
Bsa29I	ATCGAT	2 cut(s)	1503, 2922
BsaBI	GATNNNNATC	1 cut(s)	1491
BsaJI	CCNNGG	9 cut(s)	722, 1143, 1168, 1410, 1625, 2424, 2517, 2568, 2653
BsaXI	ACNNNNNCTCC	3 cut(s)	28, 496, 526
Bsc4I	CCNNNNNNNGG	7 cut(s)	632, 848, 1686, 1952, 2105, 2226, 3000
Bse118I	RCCGGY	1 cut(s)	2539
Bse1I	ACTGG	7 cut(s)	168, 871, 1753, 2022, 2053, 2505, 2994
Bse3DI	GCAATG	7 cut(s)	1908, 2181, 2390, 2431, 2580, 2863, 2910
Bse8I	GATNNNNATC	1 cut(s)	1491
BseBI	CCWGG	7 cut(s)	316, 724, 804, 1043, 1170, 2655, 2914
BseCI	ATCGAT	2 cut(s)	1503, 2922
BseDI	CCNNGG	9 cut(s)	722, 1143, 1168, 1410, 1625, 2424, 2517, 2568, 2653
BseGI	GGATG	9 cut(s)	247, 588, 718, 799, 996, 1849, 2049, 2283, 2361
BseJI	GATNNNNATC	1 cut(s)	1491
BseLI	CCNNNNNNNGG	7 cut(s)	632, 848, 1686, 1952, 2105, 2226, 3000
BseMI	GCAATG	7 cut(s)	1908, 2181, 2390, 2431, 2580, 2863, 2910
BseMII	CTCAG	6 cut(s)	442, 468, 1400, 1992, 2314, 3030
BseNI	ACTGG	7 cut(s)	168, 871, 1753, 2022, 2053, 2505, 2994
BseRI	GAGGAG	4 cut(s)	777, 1229, 1727, 2534
BseSI	GKGCMC	1 cut(s)	2878
BseXI	GCAGC	1 cut(s)	2599
BseYI	CCCAGC	1 cut(s)	2930
BsgI	GTGCAG	1 cut(s)	510
BshFI	GGCC	6 cut(s)	624, 1020, 1173, 1631, 2163, 2917
BshNI	GGYRCC	1 cut(s)	248
BshVI	ATCGAT	2 cut(s)	1503, 2922
BsiHKAI	GWGCWC	5 cut(s)	1079, 1387, 1706, 2376, 3042
BsiHKCI	CYCGRG	1 cut(s)	2425
BsiSI	CCGG	2 cut(s)	1612, 2540
BslFI	GGGAC	3 cut(s)	1840, 2254, 2442
BslI	CCNNNNNNNGG	7 cut(s)	632, 848, 1686, 1952, 2105, 2226, 3000
BsmAI	GTCTC	5 cut(s)	92, 502, 1997, 2303, 3019
BsmFI	GGGAC	3 cut(s)	1840, 2254, 2442
BsmI	GAATGC	1 cut(s)	2337
BsnI	GGCC	6 cut(s)	624, 1020, 1173, 1631, 2163, 2917
BsoBI	CYCGRG	1 cut(s)	2425
Bsp119I	TTCGAA	1 cut(s)	2773
Bsp1286I	GDGCHC	7 cut(s)	1079, 1101, 1387, 1706, 2376, 2878, 3042
Bsp1407I	TGTACA	1 cut(s)	1031
Bsp1720I	GCTNAGC	1 cut(s)	1306
Bsp19I	CCATGG	1 cut(s)	1410
BspACI	CCGC	6 cut(s)	72, 716, 757, 1310, 2510, 2537
BspANI	GGCC	6 cut(s)	624, 1020, 1173, 1631, 2163, 2917
BspCNI	CTCAG	6 cut(s)	441, 467, 1399, 1993, 2313, 3029
BspDI	ATCGAT	2 cut(s)	1503, 2922
BspHI	TCATGA	4 cut(s)	475, 1116, 1245, 2955
BspLI	GGNNCC	5 cut(s)	45, 250, 513, 1616, 2989
BspMI	ACCTGC	4 cut(s)	780, 1786, 2128, 2809
BspPI	GGATC	6 cut(s)	331, 838, 1500, 2333, 2982, 2995
BspT104I	TTCGAA	1 cut(s)	2773
BspT107I	GGYRCC	1 cut(s)	248
BsrDI	GCAATG	7 cut(s)	1908, 2181, 2390, 2431, 2580, 2863, 2910
BsrFI	RCCGGY	1 cut(s)	2539
BsrGI	TGTACA	1 cut(s)	1031
BsrI	ACTGG	7 cut(s)	168, 871, 1753, 2022, 2053, 2505, 2994
BssAI	RCCGGY	1 cut(s)	2539
BssECI	CCNNGG	9 cut(s)	722, 1143, 1168, 1410, 1625, 2424, 2517, 2568, 2653
BssNAI	GTATAC	1 cut(s)	2768
BssT1I	CCWWGG	2 cut(s)	1410, 2568
Bst1107I	GTATAC	1 cut(s)	2768
Bst2UI	CCWGG	7 cut(s)	316, 724, 804, 1043, 1170, 2655, 2914
Bst6I	CTCTTC	2 cut(s)	581, 2151
BstAUI	TGTACA	1 cut(s)	1031
BstBI	TTCGAA	1 cut(s)	2773
BstC8I	GCNNGC	5 cut(s)	77, 345, 1210, 2493, 2854
BstDSI	CCRYGG	3 cut(s)	1143, 1410, 1625
BstEII	GGTNACC	1 cut(s)	2623
BstENI	CCTNNNNNAGG	1 cut(s)	1950
BstF5I	GGATG	9 cut(s)	247, 588, 718, 799, 996, 1849, 2049, 2283, 2361
BstHHI	GCGC	2 cut(s)	699, 2497
BstMAI	GTCTC	5 cut(s)	92, 502, 1997, 2303, 3019
BstMWI	GCNNNNNNNGC	3 cut(s)	329, 1596, 2858
BstNI	CCWGG	7 cut(s)	316, 724, 804, 1043, 1170, 2655, 2914
BstNSI	RCATGY	3 cut(s)	347, 973, 1888
BstPI	GGTNACC	1 cut(s)	2623
BstSCI	CCNGG	8 cut(s)	314, 722, 802, 1041, 1168, 1611, 2653, 2912
BstSFI	CTRYAG	1 cut(s)	1122
BstSLI	GKGCMC	1 cut(s)	2878
BstV1I	GCAGC	1 cut(s)	2599
BstV2I	GAAGAC	2 cut(s)	1001, 2122
BstX2I	RGATCY	4 cut(s)	336, 469, 830, 2987
BstYI	RGATCY	4 cut(s)	336, 469, 830, 2987
BstZ17I	GTATAC	1 cut(s)	2768
Bsu15I	ATCGAT	2 cut(s)	1503, 2922
BsuI	GTATCC	1 cut(s)	620
BsuRI	GGCC	6 cut(s)	624, 1020, 1173, 1631, 2163, 2917
BsuTUI	ATCGAT	2 cut(s)	1503, 2922
BtgI	CCRYGG	3 cut(s)	1143, 1410, 1625
BtrI	CACGTC	1 cut(s)	1477
BtsCI	GGATG	9 cut(s)	247, 588, 718, 799, 996, 1849, 2049, 2283, 2361
BtsI	GCAGTG	1 cut(s)	493
BtsIMutI	CAGTG	4 cut(s)	451, 493, 1998, 2015
BveI	ACCTGC	4 cut(s)	780, 1786, 2128, 2809
Cac8I	GCNNGC	5 cut(s)	77, 345, 1210, 2493, 2854
CaiI	CAGNNNCTG	1 cut(s)	3023
CciI	TCATGA	4 cut(s)	475, 1116, 1245, 2955
CfoI	GCGC	2 cut(s)	699, 2497
Cfr10I	RCCGGY	1 cut(s)	2539
ClaI	ATCGAT	2 cut(s)	1503, 2922
CseI	GACGC	1 cut(s)	492
DraI	TTTAAA	2 cut(s)	532, 1867
DraIII	CACNNNGTG	1 cut(s)	2486
DrdI	GACNNNNNNGTC	1 cut(s)	1366
DseDI	GACNNNNNNGTC	1 cut(s)	1366
EaeI	YGGCCR	1 cut(s)	2915
Eam1104I	CTCTTC	2 cut(s)	581, 2151
EarI	CTCTTC	2 cut(s)	581, 2151
Ecl136II	GAGCTC	2 cut(s)	2374, 3040
Eco130I	CCWWGG	2 cut(s)	1410, 2568
Eco24I	GRGCYC	3 cut(s)	1101, 2376, 3042
Eco47I	GGWCC	6 cut(s)	561, 754, 1045, 1615, 1775, 3003
Eco53kI	GAGCTC	2 cut(s)	2374, 3040
Eco57I	CTGAAG	2 cut(s)	1968, 2171
Eco88I	CYCGRG	1 cut(s)	2425
Eco91I	GGTNACC	1 cut(s)	2623
EcoICRI	GAGCTC	2 cut(s)	2374, 3040
EcoNI	CCTNNNNNAGG	1 cut(s)	1950
EcoO109I	RGGNCCY	2 cut(s)	1615, 3003
EcoO65I	GGTNACC	1 cut(s)	2623
EcoRII	CCWGG	7 cut(s)	314, 722, 802, 1041, 1168, 2653, 2912
EcoT14I	CCWWGG	2 cut(s)	1410, 2568
EcoT22I	ATGCAT	1 cut(s)	2339
EcoT38I	GRGCYC	3 cut(s)	1101, 2376, 3042
ErhI	CCWWGG	2 cut(s)	1410, 2568
FalI	AAGNNNNNCTT	2 cut(s)	9, 41
FaqI	GGGAC	3 cut(s)	1840, 2254, 2442
FbaI	TGATCA	3 cut(s)	123, 1104, 1183
FblI	GTMKAC	3 cut(s)	21, 1473, 2767
Fnu4HI	GCNGC	5 cut(s)	73, 716, 2510, 2537, 2613
FokI	GGATG	9 cut(s)	254, 575, 705, 806, 1003, 1856, 2036, 2270, 2348
FriOI	GRGCYC	3 cut(s)	1101, 2376, 3042
Fsp4HI	GCNGC	5 cut(s)	73, 716, 2510, 2537, 2613
FspI	TGCGCA	1 cut(s)	698
GlaI	GCGC	2 cut(s)	698, 2496
GluI	GCNGC	5 cut(s)	73, 716, 2510, 2537, 2613
GsaI	CCCAGC	1 cut(s)	2934
GsuI	CTGGAG	1 cut(s)	1770
HaeIII	GGCC	6 cut(s)	624, 1020, 1173, 1631, 2163, 2917
HapII	CCGG	2 cut(s)	1612, 2540
HgaI	GACGC	1 cut(s)	492
HhaI	GCGC	2 cut(s)	699, 2497
Hin6I	GCGC	2 cut(s)	697, 2495
HinP1I	GCGC	2 cut(s)	697, 2495
HincII	GTYRAC	4 cut(s)	22, 597, 1474, 2314
HindII	GTYRAC	4 cut(s)	22, 597, 1474, 2314
HindIII	AAGCTT	3 cut(s)	523, 1545, 2598
HpaII	CCGG	2 cut(s)	1612, 2540
Hpy166II	GTNNAC	7 cut(s)	22, 597, 1034, 1474, 2044, 2314, 2768
Hpy8I	GTNNAC	7 cut(s)	22, 597, 1034, 1474, 2044, 2314, 2768
Hpy99I	CGWCG	4 cut(s)	486, 914, 1282, 1478
HpyCH4IV	ACGT	6 cut(s)	207, 1280, 1476, 1536, 1858, 2146
HpyF10VI	GCNNNNNNNGC	3 cut(s)	329, 1596, 2858
HpySE526I	ACGT	6 cut(s)	207, 1280, 1476, 1536, 1858, 2146
HspAI	GCGC	2 cut(s)	697, 2495
KpnI	GGTACC	1 cut(s)	252
Ksp22I	TGATCA	3 cut(s)	123, 1104, 1183
LmnI	GCTCC	8 cut(s)	517, 647, 1216, 1689, 2136, 2521, 2737, 3045
Lsp1109I	GCAGC	1 cut(s)	2599
LweI	GCATC	9 cut(s)	200, 206, 414, 727, 1569, 1924, 2324, 2667, 2805
MaeII	ACGT	6 cut(s)	207, 1280, 1476, 1536, 1858, 2146
MaeIII	GTNAC	5 cut(s)	806, 976, 2398, 2476, 2623
MflI	RGATCY	4 cut(s)	336, 469, 830, 2987
MhlI	GDGCHC	7 cut(s)	1079, 1101, 1387, 1706, 2376, 2878, 3042
MlsI	TGGCCA	1 cut(s)	2917
MluNI	TGGCCA	1 cut(s)	2917
MlyI	GAGTC	2 cut(s)	2081, 2687
MmeI	TCCRAC	6 cut(s)	539, 565, 634, 864, 929, 2064
Mox20I	TGGCCA	1 cut(s)	2917
Mph1103I	ATGCAT	1 cut(s)	2339
MroXI	GAANNNNTTC	1 cut(s)	377
MscI	TGGCCA	1 cut(s)	2917
MseI	TTAA	7 cut(s)	531, 1158, 1527, 1866, 1964, 2718, 2897
MslI	CAYNNNNRTG	5 cut(s)	677, 1539, 1682, 2417, 2996
Msp20I	TGGCCA	1 cut(s)	2917
MspI	CCGG	2 cut(s)	1612, 2540
MspR9I	CCNGG	8 cut(s)	316, 724, 804, 1043, 1170, 1613, 2655, 2914
Mva1269I	GAATGC	1 cut(s)	2337
MvaI	CCWGG	7 cut(s)	316, 724, 804, 1043, 1170, 2655, 2914
MwoI	GCNNNNNNNGC	3 cut(s)	329, 1596, 2858
NciI	CCSGG	1 cut(s)	1613
NcoI	CCATGG	1 cut(s)	1410
NlaIV	GGNNCC	5 cut(s)	45, 250, 513, 1616, 2989
NmuCI	GTSAC	4 cut(s)	806, 2398, 2476, 2623
NsbI	TGCGCA	1 cut(s)	698
NsiI	ATGCAT	1 cut(s)	2339
NspI	RCATGY	3 cut(s)	347, 973, 1888
NspV	TTCGAA	1 cut(s)	2773
OliI	CACNNNNGTG	2 cut(s)	1539, 1682
PaeI	GCATGC	1 cut(s)	347
PagI	TCATGA	4 cut(s)	475, 1116, 1245, 2955
PaqCI	CACCTGC	1 cut(s)	2809
PcsI	WCGNNNNNNNCGW	1 cut(s)	561
PctI	GAATGC	1 cut(s)	2337
PdmI	GAANNNNTTC	1 cut(s)	377
PfeI	GAWTC	9 cut(s)	31, 286, 450, 872, 1392, 2200, 2555, 2975, 3020
PflMI	CCANNNNNTGG	1 cut(s)	632
PkrI	GCNGC	5 cut(s)	74, 717, 2511, 2538, 2614
PleI	GAGTC	2 cut(s)	2080, 2686
PpsI	GAGTC	2 cut(s)	2080, 2686
PpuMI	RGGWCCY	2 cut(s)	1615, 3003
Psp124BI	GAGCTC	2 cut(s)	2376, 3042
Psp5II	RGGWCCY	2 cut(s)	1615, 3003
Psp6I	CCWGG	7 cut(s)	314, 722, 802, 1041, 1168, 2653, 2912
PspEI	GGTNACC	1 cut(s)	2623
PspFI	CCCAGC	1 cut(s)	2930
PspGI	CCWGG	7 cut(s)	314, 722, 802, 1041, 1168, 2653, 2912
PspN4I	GGNNCC	5 cut(s)	45, 250, 513, 1616, 2989
PspPPI	RGGWCCY	2 cut(s)	1615, 3003
PstNI	CAGNNNCTG	1 cut(s)	3023
PsuI	RGATCY	4 cut(s)	336, 469, 830, 2987
RseI	CAYNNNNRTG	5 cut(s)	677, 1539, 1682, 2417, 2996
SacI	GAGCTC	2 cut(s)	2376, 3042
SalI	GTCGAC	2 cut(s)	20, 1472
SaqAI	TTAA	7 cut(s)	531, 1158, 1527, 1866, 1964, 2718, 2897
SatI	GCNGC	5 cut(s)	73, 716, 2510, 2537, 2613
ScaI	AGTACT	2 cut(s)	262, 2079
SchI	GAGTC	2 cut(s)	2081, 2687
ScrFI	CCNGG	8 cut(s)	316, 724, 804, 1043, 1170, 1613, 2655, 2914
SduI	GDGCHC	7 cut(s)	1079, 1101, 1387, 1706, 2376, 2878, 3042
SfaNI	GCATC	9 cut(s)	200, 206, 414, 727, 1569, 1924, 2324, 2667, 2805
SfcI	CTRYAG	1 cut(s)	1122
SfuI	TTCGAA	1 cut(s)	2773
SinI	GGWCC	6 cut(s)	561, 754, 1045, 1615, 1775, 3003
SmiMI	CAYNNNNRTG	5 cut(s)	677, 1539, 1682, 2417, 2996
SmlI	CTYRAG	4 cut(s)	26, 1072, 1901, 2731
SmoI	CTYRAG	4 cut(s)	26, 1072, 1901, 2731
SpeI	ACTAGT	3 cut(s)	83, 257, 731
SphI	GCATGC	1 cut(s)	347
SsiI	CCGC	6 cut(s)	72, 716, 757, 1310, 2510, 2537
SspI	AATATT	4 cut(s)	352, 1429, 1791, 1968
SstI	GAGCTC	2 cut(s)	2376, 3042
StyD4I	CCNGG	8 cut(s)	314, 722, 802, 1041, 1168, 1611, 2653, 2912
StyI	CCWWGG	2 cut(s)	1410, 2568
TaiI	ACGT	6 cut(s)	210, 1283, 1479, 1539, 1861, 2149
TatI	WGTACW	5 cut(s)	260, 431, 1031, 2077, 2358
TauI	GCSGC	4 cut(s)	75, 718, 2512, 2539
TfiI	GAWTC	9 cut(s)	31, 286, 450, 872, 1392, 2200, 2555, 2975, 3020
Tru1I	TTAA	7 cut(s)	531, 1158, 1527, 1866, 1964, 2718, 2897
Tru9I	TTAA	7 cut(s)	531, 1158, 1527, 1866, 1964, 2718, 2897
TscAI	CASTG	4 cut(s)	451, 493, 2005, 2022
TseFI	GTSAC	4 cut(s)	806, 2398, 2476, 2623
TseI	GCWGC	1 cut(s)	2612
Tsp45I	GTSAC	4 cut(s)	806, 2398, 2476, 2623
TspGWI	ACGGA	1 cut(s)	1132
TspRI	CASTG	4 cut(s)	451, 493, 2005, 2022
Van91I	CCANNNNNTGG	1 cut(s)	632
VpaK11BI	GGWCC	6 cut(s)	561, 754, 1045, 1615, 1775, 3003
XagI	CCTNNNNNAGG	1 cut(s)	1950
XapI	RAATTY	2 cut(s)	899, 1660
XceI	RCATGY	3 cut(s)	347, 973, 1888
XcmI	CCANNNNNNNNNTGG	2 cut(s)	633, 1639
XmiI	GTMKAC	3 cut(s)	21, 1473, 2767
XmnI	GAANNNNTTC	1 cut(s)	377
ZrmI	AGTACT	2 cut(s)	262, 2079
Zsp2I	ATGCAT	1 cut(s)	2339

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.