Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0002542

Physical Location & Seq

Genomic Coordinates Reverse (-)

524446 .. 528129

Gene Structure 3684 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000030903

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3219 bp

ATGACAGACATCGCCAGCCCTGTGCACCAGTTCGACCTCGAGTCTCAGCATACAGTGTTGCTGAAAACTTGTAGTACTAACCCACCAATAGACCACTGGCGCTGGATTTATGTGATTCTCCAAGTGCGCAAACTCCTCATCTCATCCAGAGCCAACAAACCTAAAAACGGTTCTCAACCACCTCACCACCTCTCATCAGCTACAATCCCTTATGCTATTATCAGCACCAGCACTACTGCTGGTGATGCACGAGATGCGGACTCGCAACTGACATCAAGGACTACAGAAATCTTACCTGCCTCGCCTCAATTTTCTGGAGATGTCCATCAGATCGGGGAGGATGATGGTAGTACTGCTGCTACTGATGCAAAACTCCAGCTTACATATATTGCCAAGATAGTCACGGAGAAAGACTTTGAGTCCTTTCAGAAGTTTGGATGCGTCCAAGGAATTGCAGAGGCACTTAATAGCGATTTACAGAATGGAATCCCAGGCAATCAAGAAGATGTGAGTTCTCGCCGCCCAGTTAGTGCCCTCTTCACAACACAGGCTCCTGTTCAAGGCTTCTTCAAACTGCTCCTACAGTCCAGCAACAACTACATCATTTTCCTTCTCTTGGTGGCTGCAGTGCTGTCGATTGGCTTTGGTATGAAGGAGGAAGGCCCAAGCACCGGTTGGTATGAAGGGGCCATCATACTGTTTGTCATCATCATACTCGTTGTTGCGCCTTCCATACGCGACTTTTGGCTTCAAAATCCACAGAGCCATGCAATGAGCCAGACTGCAGGGGCCATTAAAATGGTGGTTGAGGTCATCAGAGGGGGATCCCAATGTGAAATACCCATATGTGCTGTTGTTTCAGGCGACATAGTGTGCTTGAAACGGGGATCTATAGTTCCCGGTGATGGTTTGTTTGTTTCTGGTGAGTTCTTGGTATTGGATGATGGTGCAGTGGCAACCATTTATGACAAGAAACCATTCTTGTTCTGTGGTTCAAAGGTGGTTGGTGGAAGTGGTCGCATGCTTGTAACGTCTGTGGGCATGGACACAGCATGGGGTGAGCTGATGAAGAAAGTAGCCAATACTCCTAACCGGGCTCATTTACCGGCTCAACTTGATAAGGTCAGCACAGGCACGCAGATTATAGGGATCTCAATCTCTATTCTCATCCTGGTAGTGTTGTTCTTCCGTTTCATGGTTGAAAGGGAAGACAATCACTCTGGCCTCCCAGACCTTAAGGGGAAGTCAACTGCAAGCAAGGAGATAATGAATTTAATTGAGAAAATTGTGATGAAACCAAGTGGTCGGATCAGTATCCTAACGACATCTCTGGCTATATTCCTCCTAGGAGTAGTGGAAGGAATACCTATTTTTGTTACACTTGCCATAAGGTATTGGAATAAGAAGACTTTATCTGGAAAGGCCACTGCTCAAGGGACTTTGGCGTGTGTCACCATGGGCTCTATCACAACTATTTGCACTGACAAAACTGGCGTGCTGAAATTGTCAGTAGACAAGTGCTACATCGGGGATGAAGTCATAGAAACCGACTATGTCACTAGAATAGACACACATGTTCGTGAAGCTTTGTGCAATGGAATCAGCACACCCCTTCTGATGCCAAACCCTCATTGTAGCTCGAGAGAGGACCCGCTTCTTATTTCCTGGGCTAACCTCAATTTGGGAATGCAACATGAGATTTTGAGGCAAAATTGCACCATCCTGGAGATCAAAGGATTGAGCACCAACGACGAAGGCAGTGGAGTACTGATGAGAAGAAGCAGTGCTGACAACGAAGGAGATATGCGCTTGCATTGGAAAGGACCTGCAACAACAATATTAGCCATGTGTTCGCACTACAATGACAACAAAGGGGAAACAAAGGTCATGGATGAACAAAGTATATTGGTTTTTAACCAAATTATAGAGGGCATGCAGTCCAAATATCTCAAGACCATTGCATTTGCTTACAAACAGACTGAGGTTTCAATGCTAGAAGAAAACAGCTTAATCTTAATAGCACTGCTAGGTGTGAGGTATTCATGCTGCCCAGATATAGTGGAAGCTTGTCAAGAAGCAGGAGTCAACATCATACTGGTCTCAGAAGATAATGTTTCGGACCTGAAAGACATTGCTCGTGAGTGTGGAATACTTCTGAACCCAAACAGATTGGTAGTTGAAGGTAAAAGTTTTCGAAATAGTGCCCAAGAAGACAGGATGGACATGGCAGATAAAATATGTGTGATGGGAAACTCCATTCCTTCTGACAGGCTCCTTTTGGTGCAATGTCTGAAAGAAAGAGGCCATGTCGTAGCAATGGTTGGAGTCGGAACAAATGATGCTCCAACTCTGAAAGAAGCTGATGTGGGCATTGCAATGGGGACTTGGAGTAATGGAGTGGCAAGAGAGAGTTCTGACATCATCATCTGGGACGGAAATGATAGTTTCTTGGTCACCATTATTGGGCGTGGAAGAAGCATATACAATAACATTCAGAAGTACATTCAACTTGAGCTCACCATGAATATATCATGGCTGTTGATAGCATCCGCAACCACAATGTGTTTAGGACAAAGTGCAATTGCAGCCATACAATTGGTCTGGGCAAACATGATTGTGACTCTTCTAGGTGGGCTTGCTCTATTGATGGAGCCTCCAACAGAGGAACTAATGAAAAAGGCACCGGTCAAGCGAAATGAATCTCTCATTAGCAAGGGAATGTGGAGAAACATAGTCAGTCAAGCTTTATATCAGACTACCATCTTGGTCGCCTTTCAATTCAAAGGACCAACTATCCCAGGCATCAGCAAGAAGGTCAGTGAGTCCATTGTTTTCAACAGTTTTGTTCTCTGTCAGGTATTCAACCAAGTGAATTCAAGGGAACTCGAGAAGAAAAACGTATTTAGGGGCATATTTCACAATCTGTGGTTTTGGGTGGCTGTGGGTGCCATTCTTATCCTGCAGGTGGGTTTTGTTGAGATTGCACACATTCTGGTTGGCAATGCAAGGTTGAATTGGGCAGAGTGGGGTATCTGTCTTCTAATTGGGATGGTTTCATGGGCAATTGATTTGGTTGTTAAGTTTGCAACCAGTTTCATACTTAGCTCAGGATCCATTAGTATGCCTATGACACCCTCTAATGTTTCAGAATCCGCTGCTAATCTAGAGCTCCCACTCATTAATGAAATTCCACATGAATCCCATCTTAGGCTGTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase Hydrolase

Protein Analysis

1073

Amino Acids

117.22

Weight (kDa)

5.89

Isoelectric Point (pI)

34.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	268 - 359	4.4e-10	P-type ATPase actuator domain
Cation_ATPase	PF13246	561 - 664	2.1e-08	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	859 - 1028	5.5e-38	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002542)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g00630 FvH4_3g13880
prunus_persica	Prupe.4G007300_v2.0.a1 Prupe.4G007300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0000841 RchiOBHm_Chr5g0000871
rosa_laevigata	RLG00000030901 RLG00000030902 RLG00000030903 RLG00000032693 RLG00000032697
rosa_multiflora	Rmu_co8361431.1_g000001 Rmu_sc0000255.1_g000013 Rmu_sc0000255.1_g000017 Rmu_sc0001979.1_g000005 Rmu_sc0002085.1_g000003 Rmu_sc0002085.1_g000004 Rmu_sc0010050.1_g000003 Rmu_sc0010050.1_g000006 Rmu_sc0019378.1_g000001 Rmu_sc0034511.1_g000001
rosa_roxburghii	Rroxscaffold_1G00056260 Rroxscaffold_1G00075400
rosa_rugosa	Rorug04G0387000 Rorug04G0387100 Rorug04G0387200 Rorug05G0076900
rosa_samantha	Rh5AG006900 Rh5AG007000 Rh5AG163100 Rh5BG009000 Rh5BG009100 Rh5BG162300 Rh5CG007600 Rh5CG007700 Rh5CG177100 Rh5DG007600 Rh5DG163600
rosa_wichuraiana	Rw5G000710 Rw5G000730 Rw5G014710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2953
Acc16I	TGCGCA	1 cut(s)	128
Acc36I	ACCTGC	3 cut(s)	304, 1834, 2953
AccB1I	GGYRCC	2 cut(s)	2680, 2945
AccI	GTMKAC	1 cut(s)	1512
AccII	CGCG	1 cut(s)	738
AciI	CCGC	5 cut(s)	257, 520, 1652, 2550, 3153
AclWI	GGATC	7 cut(s)	819, 832, 895, 1157, 1316, 3105, 3118
AcsI	RAATTY	3 cut(s)	1270, 2872, 3186
AdeI	CACNNNGTG	1 cut(s)	2562
AfaI	GTAC	4 cut(s)	76, 352, 1767, 2501
AflII	CTTAAG	1 cut(s)	1235
AflIII	ACRYGT	1 cut(s)	1573
AgeI	ACCGGT	2 cut(s)	671, 2683
AhdI	GACNNNNNGTC	2 cut(s)	40, 418
AjnI	CCWGG	5 cut(s)	490, 1170, 1664, 1722, 2797
AloI	GAACNNNNNNTCC	2 cut(s)	879, 911
Alw21I	GWGCWC	4 cut(s)	27, 1745, 2517, 3171
Alw26I	GTCTC	2 cut(s)	48, 2104
Alw44I	GTGCAC	1 cut(s)	23
AlwI	GGATC	7 cut(s)	819, 832, 895, 1157, 1316, 3105, 3118
Ama87I	CYCGRG	3 cut(s)	38, 1639, 2885
AoxI	GGCC	6 cut(s)	661, 687, 789, 1222, 1422, 2302
ApaLI	GTGCAC	1 cut(s)	23
ApeKI	GCWGC	5 cut(s)	356, 623, 2046, 2585, 3155
ApoI	RAATTY	3 cut(s)	1270, 2872, 3186
AseI	ATTAAT	1 cut(s)	3180
AsiGI	ACCGGT	2 cut(s)	671, 2683
Asp700I	GAANNNNTTC	1 cut(s)	977
AspA2I	CCTAGG	1 cut(s)	1345
AspLEI	GCGC	4 cut(s)	102, 129, 727, 1809
AspS9I	GGNCC	7 cut(s)	662, 687, 789, 1648, 1823, 2119, 2786
AsuC2I	CCSGG	2 cut(s)	900, 1094
AsuHPI	GGTGA	8 cut(s)	176, 254, 914, 935, 1070, 1444, 2446, 2509
AsuII	TTCGAA	1 cut(s)	2194
AvaI	CYCGRG	3 cut(s)	38, 1639, 2885
AvaII	GGWCC	4 cut(s)	1648, 1823, 2119, 2786
AvrII	CCTAGG	1 cut(s)	1345
BaeGI	GKGCMC	3 cut(s)	27, 535, 2206
BamHI	GGATCC	2 cut(s)	824, 3110
BanI	GGYRCC	2 cut(s)	2680, 2945
BanII	GRGCYC	4 cut(s)	1099, 1463, 2517, 3171
BauI	CACGAG	2 cut(s)	249, 2136
BbsI	GAAGAC	4 cut(s)	1215, 1412, 2217, 3029
Bbv12I	GWGCWC	4 cut(s)	27, 1745, 2517, 3171
BbvI	GCAGC	5 cut(s)	343, 610, 2033, 2597, 3142
BcgI	CGANNNNNNTGC	2 cut(s)	615, 649
BciT130I	CCWGG	5 cut(s)	492, 1172, 1666, 1724, 2799
BciVI	GTATCC	1 cut(s)	1325
BcnI	CCSGG	2 cut(s)	900, 1094
BcoDI	GTCTC	2 cut(s)	48, 2104
BfaI	CTAG	6 cut(s)	1346, 1560, 1994, 2027, 2627, 3164
BfmI	CTRYAG	6 cut(s)	282, 581, 624, 783, 891, 2960
BfoI	RGCGCY	1 cut(s)	103
BfrI	CTTAAG	1 cut(s)	1235
BfuAI	ACCTGC	3 cut(s)	304, 1834, 2953
BfuI	GTATCC	1 cut(s)	1325
BisI	GCNGC	6 cut(s)	357, 520, 624, 2047, 2586, 3156
BlnI	CCTAGG	1 cut(s)	1345
BlsI	GCNGC	6 cut(s)	358, 521, 625, 2048, 2587, 3157
BmcAI	AGTACT	3 cut(s)	76, 352, 1767
Bme1390I	CCNGG	7 cut(s)	492, 900, 1094, 1172, 1666, 1724, 2799
Bme18I	GGWCC	4 cut(s)	1648, 1823, 2119, 2786
BmeRI	GACNNNNNGTC	2 cut(s)	40, 418
BmeT110I	CYCGRG	3 cut(s)	38, 1639, 2885
BmgT120I	GGNCC	7 cut(s)	662, 687, 789, 1648, 1823, 2119, 2786
BmrFI	CCNGG	7 cut(s)	492, 900, 1094, 1172, 1666, 1724, 2799
BmrI	ACTGGG	1 cut(s)	518
BmsI	GCATC	8 cut(s)	235, 244, 355, 428, 1608, 2329, 2555, 2811
BmuI	ACTGGG	1 cut(s)	518
BpiI	GAAGAC	4 cut(s)	1215, 1412, 2217, 3029
BpmI	CTGGAG	3 cut(s)	336, 359, 1745
Bpu10I	CCTNAGC	1 cut(s)	3106
Bpu14I	TTCGAA	1 cut(s)	2194
BpuEI	CTTGAG	3 cut(s)	1416, 1934, 2531
BpuMI	CCSGG	2 cut(s)	900, 1094
BsaBI	GATNNNNATC	3 cut(s)	324, 1154, 1313
BsaI	GGTCTC	1 cut(s)	2104
BsaJI	CCNNGG	6 cut(s)	445, 490, 1345, 1455, 1665, 2797
BsaWI	WCCGGW	2 cut(s)	671, 2683
BsaXI	ACNNNNNCTCC	2 cut(s)	535, 565
Bse118I	RCCGGY	3 cut(s)	671, 1105, 2683
Bse1I	ACTGG	6 cut(s)	28, 101, 524, 1495, 2100, 3090
Bse3DI	GCAATG	9 cut(s)	777, 1600, 1956, 2130, 2291, 2322, 2370, 2382, 3007
Bse8I	GATNNNNATC	3 cut(s)	324, 1154, 1313
BseBI	CCWGG	5 cut(s)	492, 1172, 1666, 1724, 2799
BseDI	CCNNGG	6 cut(s)	445, 490, 1345, 1455, 1665, 2797
BseJI	GATNNNNATC	3 cut(s)	324, 1154, 1313
BseMI	GCAATG	9 cut(s)	777, 1600, 1956, 2130, 2291, 2322, 2370, 2382, 3007
BseMII	CTCAG	4 cut(s)	59, 1971, 2115, 3120
BseNI	ACTGG	6 cut(s)	28, 101, 524, 1495, 2100, 3090
BseRI	GAGGAG	1 cut(s)	125
BseSI	GKGCMC	3 cut(s)	27, 535, 2206
BseXI	GCAGC	5 cut(s)	343, 610, 2033, 2597, 3142
BsgI	GTGCAG	1 cut(s)	969
Bsh1236I	CGCG	1 cut(s)	738
BshFI	GGCC	6 cut(s)	663, 689, 791, 1224, 1424, 2304
BshNI	GGYRCC	2 cut(s)	2680, 2945
BshTI	ACCGGT	2 cut(s)	671, 2683
BsiHKAI	GWGCWC	4 cut(s)	27, 1745, 2517, 3171
BsiHKCI	CYCGRG	3 cut(s)	38, 1639, 2885
BsiSI	CCGG	5 cut(s)	672, 900, 1093, 1106, 2684
BslFI	GGGAC	3 cut(s)	1450, 2395, 2444
BsmAI	GTCTC	2 cut(s)	48, 2104
BsmFI	GGGAC	3 cut(s)	1450, 2395, 2444
BsmI	GAATGC	1 cut(s)	1692
BsnI	GGCC	6 cut(s)	663, 689, 791, 1224, 1424, 2304
Bso31I	GGTCTC	1 cut(s)	2104
BsoBI	CYCGRG	3 cut(s)	38, 1639, 2885
Bsp119I	TTCGAA	1 cut(s)	2194
Bsp1286I	GDGCHC	8 cut(s)	27, 535, 1099, 1463, 1745, 2206, 2517, 3171
Bsp143I	GATC	7 cut(s)	330, 824, 887, 1149, 1308, 1728, 3110
Bsp19I	CCATGG	1 cut(s)	1455
BspACI	CCGC	5 cut(s)	257, 520, 1652, 2550, 3153
BspANI	GGCC	6 cut(s)	663, 689, 791, 1224, 1424, 2304
BspCNI	CTCAG	4 cut(s)	58, 1972, 2114, 3119
BspFNI	CGCG	1 cut(s)	738
BspMAI	CTGCAG	3 cut(s)	628, 787, 2964
BspMI	ACCTGC	3 cut(s)	304, 1834, 2953
BspPI	GGATC	7 cut(s)	819, 832, 895, 1157, 1316, 3105, 3118
BspT104I	TTCGAA	1 cut(s)	2194
BspT107I	GGYRCC	2 cut(s)	2680, 2945
BspTI	CTTAAG	1 cut(s)	1235
BspTNI	GGTCTC	1 cut(s)	2104
BsrDI	GCAATG	9 cut(s)	777, 1600, 1956, 2130, 2291, 2322, 2370, 2382, 3007
BsrFI	RCCGGY	3 cut(s)	671, 1105, 2683
BsrI	ACTGG	6 cut(s)	28, 101, 524, 1495, 2100, 3090
BssAI	RCCGGY	3 cut(s)	671, 1105, 2683
BssECI	CCNNGG	6 cut(s)	445, 490, 1345, 1455, 1665, 2797
BssMI	GATC	7 cut(s)	330, 824, 887, 1149, 1308, 1728, 3110
BssSI	CACGAG	2 cut(s)	249, 2136
BssT1I	CCWWGG	3 cut(s)	445, 1345, 1455
Bst2BI	CACGAG	2 cut(s)	249, 2136
Bst2UI	CCWGG	5 cut(s)	492, 1172, 1666, 1724, 2799
Bst4CI	ACNGT	5 cut(s)	55, 170, 585, 699, 2840
Bst6I	CTCTTC	2 cut(s)	542, 2628
BstAFI	CTTAAG	1 cut(s)	1235
BstAPI	GCANNNNNTGC	1 cut(s)	254
BstBI	TTCGAA	1 cut(s)	2194
BstC8I	GCNNGC	8 cut(s)	16, 1022, 1136, 1255, 1496, 1811, 1934, 2637
BstDEI	CTNAG	6 cut(s)	45, 1980, 2101, 3101, 3106, 3206
BstDSI	CCRYGG	1 cut(s)	1455
BstEII	GGTNACC	1 cut(s)	2452
BstENI	CCTNNNNNAGG	1 cut(s)	558
BstFNI	CGCG	1 cut(s)	738
BstH2I	RGCGCY	1 cut(s)	103
BstHHI	GCGC	4 cut(s)	102, 129, 727, 1809
BstKTI	GATC	7 cut(s)	333, 827, 890, 1152, 1311, 1731, 3113
BstMAI	GTCTC	2 cut(s)	48, 2104
BstMBI	GATC	7 cut(s)	330, 824, 887, 1149, 1308, 1728, 3110
BstMWI	GCNNNNNNNGC	5 cut(s)	245, 254, 365, 2585, 2945
BstNI	CCWGG	5 cut(s)	492, 1172, 1666, 1724, 2799
BstNSI	RCATGY	3 cut(s)	1024, 1577, 1936
BstPI	GGTNACC	1 cut(s)	2452
BstSCI	CCNGG	7 cut(s)	490, 898, 1092, 1170, 1664, 1722, 2797
BstSFI	CTRYAG	6 cut(s)	282, 581, 624, 783, 891, 2960
BstSLI	GKGCMC	3 cut(s)	27, 535, 2206
BstUI	CGCG	1 cut(s)	738
BstV1I	GCAGC	5 cut(s)	343, 610, 2033, 2597, 3142
BstV2I	GAAGAC	4 cut(s)	1215, 1412, 2217, 3029
BstX2I	RGATCY	4 cut(s)	824, 887, 1149, 3110
BstXI	CCANNNNNNTGG	2 cut(s)	799, 2596
BstYI	RGATCY	4 cut(s)	824, 887, 1149, 3110
BsuI	GTATCC	1 cut(s)	1325
BsuRI	GGCC	6 cut(s)	663, 689, 791, 1224, 1424, 2304
BtgI	CCRYGG	1 cut(s)	1455
BtsI	GCAGTG	6 cut(s)	633, 957, 1425, 1765, 1789, 2021
BveI	ACCTGC	3 cut(s)	304, 1834, 2953
Cac8I	GCNNGC	8 cut(s)	16, 1022, 1136, 1255, 1496, 1811, 1934, 2637
CfoI	GCGC	4 cut(s)	102, 129, 727, 1809
Cfr10I	RCCGGY	3 cut(s)	671, 1105, 2683
Cfr13I	GGNCC	7 cut(s)	662, 687, 789, 1648, 1823, 2119, 2786
CseI	GACGC	1 cut(s)	430
Csp6I	GTAC	4 cut(s)	75, 351, 1766, 2500
CspAI	ACCGGT	2 cut(s)	671, 2683
CviQI	GTAC	4 cut(s)	75, 351, 1766, 2500
DdeI	CTNAG	6 cut(s)	45, 1980, 2101, 3101, 3106, 3206
DpnI	GATC	7 cut(s)	332, 826, 889, 1151, 1310, 1730, 3112
DpnII	GATC	7 cut(s)	330, 824, 887, 1149, 1308, 1728, 3110
DraIII	CACNNNGTG	1 cut(s)	2562
DriI	GACNNNNNGTC	2 cut(s)	40, 418
Eam1104I	CTCTTC	2 cut(s)	542, 2628
Eam1105I	GACNNNNNGTC	2 cut(s)	40, 418
EarI	CTCTTC	2 cut(s)	542, 2628
Ecl136II	GAGCTC	2 cut(s)	2515, 3169
Eco130I	CCWWGG	3 cut(s)	445, 1345, 1455
Eco24I	GRGCYC	4 cut(s)	1099, 1463, 2517, 3171
Eco31I	GGTCTC	1 cut(s)	2104
Eco47I	GGWCC	4 cut(s)	1648, 1823, 2119, 2786
Eco53kI	GAGCTC	2 cut(s)	2515, 3169
Eco88I	CYCGRG	3 cut(s)	38, 1639, 2885
Eco91I	GGTNACC	1 cut(s)	2452
EcoICRI	GAGCTC	2 cut(s)	2515, 3169
EcoNI	CCTNNNNNAGG	1 cut(s)	558
EcoO109I	RGGNCCY	2 cut(s)	1648, 1823
EcoO65I	GGTNACC	1 cut(s)	2452
EcoRI	GAATTC	1 cut(s)	2872
EcoRII	CCWGG	5 cut(s)	490, 1170, 1664, 1722, 2797
EcoT14I	CCWWGG	3 cut(s)	445, 1345, 1455
EcoT38I	GRGCYC	4 cut(s)	1099, 1463, 2517, 3171
ErhI	CCWWGG	3 cut(s)	445, 1345, 1455
FaqI	GGGAC	3 cut(s)	1450, 2395, 2444
FauI	CCCGC	1 cut(s)	1659
FauNDI	CATATG	1 cut(s)	845
FblI	GTMKAC	1 cut(s)	1512
Fnu4HI	GCNGC	6 cut(s)	357, 520, 624, 2047, 2586, 3156
FriOI	GRGCYC	4 cut(s)	1099, 1463, 2517, 3171
Fsp4HI	GCNGC	6 cut(s)	357, 520, 624, 2047, 2586, 3156
FspBI	CTAG	6 cut(s)	1346, 1560, 1994, 2027, 2627, 3164
FspI	TGCGCA	1 cut(s)	128
GlaI	GCGC	4 cut(s)	101, 128, 726, 1808
GluI	GCNGC	6 cut(s)	357, 520, 624, 2047, 2586, 3156
GsuI	CTGGAG	3 cut(s)	336, 359, 1745
HaeII	RGCGCY	1 cut(s)	103
HaeIII	GGCC	6 cut(s)	663, 689, 791, 1224, 1424, 2304
HapII	CCGG	5 cut(s)	672, 900, 1093, 1106, 2684
HgaI	GACGC	1 cut(s)	430
HhaI	GCGC	4 cut(s)	102, 129, 727, 1809
Hin6I	GCGC	4 cut(s)	100, 127, 725, 1807
HinP1I	GCGC	4 cut(s)	100, 127, 725, 1807
HincII	GTYRAC	2 cut(s)	1248, 2086
HindII	GTYRAC	2 cut(s)	1248, 2086
HindIII	AAGCTT	3 cut(s)	1584, 2064, 2742
HpaII	CCGG	5 cut(s)	672, 900, 1093, 1106, 2684
HphI	GGTGA	8 cut(s)	176, 254, 914, 935, 1070, 1444, 2446, 2509
Hpy166II	GTNNAC	4 cut(s)	25, 1248, 1513, 2086
Hpy8I	GTNNAC	4 cut(s)	25, 1248, 1513, 2086
Hpy99I	CGWCG	1 cut(s)	1754
HpyCH4III	ACNGT	5 cut(s)	55, 170, 585, 699, 2840
HpyCH4IV	ACGT	2 cut(s)	1031, 2898
HpyF10VI	GCNNNNNNNGC	5 cut(s)	245, 254, 365, 2585, 2945
HpyF3I	CTNAG	6 cut(s)	45, 1980, 2101, 3101, 3106, 3206
HpySE526I	ACGT	2 cut(s)	1031, 2898
HspAI	GCGC	4 cut(s)	100, 127, 725, 1807
Kzo9I	GATC	7 cut(s)	330, 824, 887, 1149, 1308, 1728, 3110
LmnI	GCTCC	6 cut(s)	556, 582, 2277, 2347, 2650, 3174
Lsp1109I	GCAGC	5 cut(s)	343, 610, 2033, 2597, 3142
LweI	GCATC	8 cut(s)	235, 244, 355, 428, 1608, 2329, 2555, 2811
MaeI	CTAG	6 cut(s)	1346, 1560, 1994, 2027, 2627, 3164
MaeII	ACGT	2 cut(s)	1031, 2898
MaeIII	GTNAC	7 cut(s)	400, 1027, 1375, 1450, 1555, 2452, 2617
MalI	GATC	7 cut(s)	332, 826, 889, 1151, 1310, 1730, 3112
MboI	GATC	7 cut(s)	330, 824, 887, 1149, 1308, 1728, 3110
MfeI	CAATTG	3 cut(s)	2580, 2594, 3063
MflI	RGATCY	4 cut(s)	824, 887, 1149, 3110
MhlI	GDGCHC	8 cut(s)	27, 535, 1099, 1463, 1745, 2206, 2517, 3171
MlyI	GAGTC	7 cut(s)	50, 254, 428, 2091, 2334, 2614, 2831
MmeI	TCCRAC	5 cut(s)	1286, 2302, 2308, 2369, 2681
MroXI	GAANNNNTTC	1 cut(s)	977
MslI	CAYNNNNRTG	6 cut(s)	797, 1578, 1860, 2375, 2615, 3119
MspA1I	CMGCKG	1 cut(s)	3155
MspCI	CTTAAG	1 cut(s)	1235
MspI	CCGG	5 cut(s)	672, 900, 1093, 1106, 2684
MspR9I	CCNGG	7 cut(s)	492, 900, 1094, 1172, 1666, 1724, 2799
MunI	CAATTG	3 cut(s)	2580, 2594, 3063
Mva1269I	GAATGC	1 cut(s)	1692
MvaI	CCWGG	5 cut(s)	492, 1172, 1666, 1724, 2799
MvnI	CGCG	1 cut(s)	738
MwoI	GCNNNNNNNGC	5 cut(s)	245, 254, 365, 2585, 2945
NciI	CCSGG	2 cut(s)	900, 1094
NcoI	CCATGG	1 cut(s)	1455
NdeI	CATATG	1 cut(s)	845
NdeII	GATC	7 cut(s)	330, 824, 887, 1149, 1308, 1728, 3110
NmuCI	GTSAC	5 cut(s)	400, 1450, 1555, 2452, 2617
NsbI	TGCGCA	1 cut(s)	128
NspI	RCATGY	3 cut(s)	1024, 1577, 1936
NspV	TTCGAA	1 cut(s)	2194
PaeI	GCATGC	2 cut(s)	1024, 1936
PaeR7I	CTCGAG	3 cut(s)	38, 1639, 2885
PaqCI	CACCTGC	1 cut(s)	2953
PciI	ACATGT	1 cut(s)	1573
PctI	GAATGC	1 cut(s)	1692
PdmI	GAANNNNTTC	1 cut(s)	977
PfeI	GAWTC	6 cut(s)	115, 486, 1599, 2699, 3149, 3197
PflFI	GACNNNGTC	1 cut(s)	1553
PfoI	TCCNGGA	1 cut(s)	1722
PinAI	ACCGGT	2 cut(s)	671, 2683
PkrI	GCNGC	6 cut(s)	358, 521, 625, 2048, 2587, 3157
PleI	GAGTC	7 cut(s)	49, 254, 427, 2090, 2333, 2614, 2830
PpsI	GAGTC	7 cut(s)	49, 254, 427, 2090, 2333, 2614, 2830
PpuMI	RGGWCCY	2 cut(s)	1648, 1823
PscI	ACATGT	1 cut(s)	1573
PshBI	ATTAAT	1 cut(s)	3180
Psp124BI	GAGCTC	2 cut(s)	2517, 3171
Psp5II	RGGWCCY	2 cut(s)	1648, 1823
Psp6I	CCWGG	5 cut(s)	490, 1170, 1664, 1722, 2797
PspEI	GGTNACC	1 cut(s)	2452
PspGI	CCWGG	5 cut(s)	490, 1170, 1664, 1722, 2797
PspPI	GGNCC	7 cut(s)	662, 687, 789, 1648, 1823, 2119, 2786
PspPPI	RGGWCCY	2 cut(s)	1648, 1823
PspXI	VCTCGAGB	1 cut(s)	38
PstI	CTGCAG	3 cut(s)	628, 787, 2964
PsuI	RGATCY	4 cut(s)	824, 887, 1149, 3110
PsyI	GACNNNGTC	1 cut(s)	1553
RsaI	GTAC	4 cut(s)	76, 352, 1767, 2501
RsaNI	GTAC	4 cut(s)	75, 351, 1766, 2500
RseI	CAYNNNNRTG	6 cut(s)	797, 1578, 1860, 2375, 2615, 3119
SacI	GAGCTC	2 cut(s)	2517, 3171
SatI	GCNGC	6 cut(s)	357, 520, 624, 2047, 2586, 3156
Sau3AI	GATC	7 cut(s)	330, 824, 887, 1149, 1308, 1728, 3110
Sau96I	GGNCC	7 cut(s)	662, 687, 789, 1648, 1823, 2119, 2786
SbfI	CCTGCAGG	1 cut(s)	2964
ScaI	AGTACT	3 cut(s)	76, 352, 1767
SchI	GAGTC	7 cut(s)	50, 254, 428, 2091, 2334, 2614, 2831
ScrFI	CCNGG	7 cut(s)	492, 900, 1094, 1172, 1666, 1724, 2799
SdaI	CCTGCAGG	1 cut(s)	2964
SduI	GDGCHC	8 cut(s)	27, 535, 1099, 1463, 1745, 2206, 2517, 3171
SfaNI	GCATC	8 cut(s)	235, 244, 355, 428, 1608, 2329, 2555, 2811
SfcI	CTRYAG	6 cut(s)	282, 581, 624, 783, 891, 2960
Sfr274I	CTCGAG	3 cut(s)	38, 1639, 2885
SfuI	TTCGAA	1 cut(s)	2194
SinI	GGWCC	4 cut(s)	1648, 1823, 2119, 2786
SlaI	CTCGAG	3 cut(s)	38, 1639, 2885
SmiMI	CAYNNNNRTG	6 cut(s)	797, 1578, 1860, 2375, 2615, 3119
SmlI	CTYRAG	7 cut(s)	38, 1235, 1431, 1639, 1949, 2510, 2885
SmoI	CTYRAG	7 cut(s)	38, 1235, 1431, 1639, 1949, 2510, 2885
SphI	GCATGC	2 cut(s)	1024, 1936
Sse8387I	CCTGCAGG	1 cut(s)	2964
SsiI	CCGC	5 cut(s)	257, 520, 1652, 2550, 3153
SspI	AATATT	1 cut(s)	1839
SspMI	CTAG	6 cut(s)	1346, 1560, 1994, 2027, 2627, 3164
SstI	GAGCTC	2 cut(s)	2517, 3171
StyD4I	CCNGG	7 cut(s)	490, 898, 1092, 1170, 1664, 1722, 2797
StyI	CCWWGG	3 cut(s)	445, 1345, 1455
TaaI	ACNGT	5 cut(s)	55, 170, 585, 699, 2840
TaiI	ACGT	2 cut(s)	1034, 2901
TaqI	TCGA	6 cut(s)	33, 39, 635, 1640, 2194, 2886
TatI	WGTACW	4 cut(s)	74, 350, 1765, 2499
TauI	GCSGC	1 cut(s)	522
TfiI	GAWTC	6 cut(s)	115, 486, 1599, 2699, 3149, 3197
TseFI	GTSAC	5 cut(s)	400, 1450, 1555, 2452, 2617
TseI	GCWGC	5 cut(s)	356, 623, 2046, 2585, 3155
Tsp45I	GTSAC	5 cut(s)	400, 1450, 1555, 2452, 2617
TspGWI	ACGGA	3 cut(s)	419, 1178, 2448
Tth111I	GACNNNGTC	1 cut(s)	1553
Vha464I	CTTAAG	1 cut(s)	1235
VneI	GTGCAC	1 cut(s)	23
VpaK11BI	GGWCC	4 cut(s)	1648, 1823, 2119, 2786
VspI	ATTAAT	1 cut(s)	3180
XagI	CCTNNNNNAGG	1 cut(s)	558
XapI	RAATTY	3 cut(s)	1270, 2872, 3186
XbaI	TCTAGA	1 cut(s)	3163
XceI	RCATGY	3 cut(s)	1024, 1577, 1936
XcmI	CCANNNNNNNNNTGG	3 cut(s)	93, 672, 799
XhoI	CTCGAG	3 cut(s)	38, 1639, 2885
XmaJI	CCTAGG	1 cut(s)	1345
XmiI	GTMKAC	1 cut(s)	1512
XmnI	GAANNNNTTC	1 cut(s)	977
XspI	CTAG	6 cut(s)	1346, 1560, 1994, 2027, 2627, 3164
ZrmI	AGTACT	3 cut(s)	76, 352, 1767

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.