Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0002542

Physical Location & Seq

Genomic Coordinates Reverse (-)

78028841 .. 78037858

Gene Structure 9018 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00056260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3081 bp

ATGGGGAGTTCTGGTGAAGCCGACCTTGAGAATCAACAAACGGTTCCCCATGTGGCGACTGATCGTGGAGGCGGCTGGCTAACTAGTCTCTTGAATGTAATTCATTCATTTCGCCAGTTTCTGATCATAAAACCTTCTTCTGCTGATGATGAAAGTGAGGAGACTAATGTCACTAGACCAAAACGATACTCGGAACTGGAACTTACAAGAACTACACACCTTGCATCTGCATCACCTCAGGTTTCTAAAGCAGTATCAAGTTTGAGCGTGGAGGATGATAGTACCATCACTACTACTGCTACCGTTTCTGACCAAGTGAATCTGAACATTGAGGATGATATTACTACCAGGATTGCTAATGAGGCTAAGATCCAGCATGCAAATATTTCCAAGATAGTAAAGGAGAAGGACTTCGCTACCTTACAGAAGTTTGGAGGCATCAAAGGAATTGCAGAGGCACTCAGTACAGATTTACACAGCGGAATCCCAGGTGATGAACAAGATTTTCATGATCGACGCAGTGCAGTAGTCTCAACAACACAGGCTCCAACTCCAAGCTTCTTTAAACTTCTCCGACAATCTTGTTTCGATTGGACTGTTTTCCTTCTCTTCGTGTGTGTCATCCTGTCAACTGTATTTGGTATCCAGCAGGAAGGCCCAAGTAATGGTTGGCTTGAAGGAGCTGTCGGAATGTTTGCGATCATCATACTTGTGGTTGTACCTACAATACGCAAGTATTTCTCAGTGCCGCATCCCGGGAATACTAGTCAAATGAAAGTTAAGGTCCGCAGAGGAGGAAACCTGCTTCATATCTCCCCATCGGATGTTGTGCCAGGTGACATAGTATGCTTGGAATTGGGATCTCTAATTCCTGCTGATGGTTTGTTCGTTTCGACTGGTGAATCCTTAGTGTTGGAAGATGGTATGGAATTTCCAACCGTCGATGACAGCAGTCCTTTTCTGGTTTATGGTGCAAGGGTGGTATATGGTAATGGTCGCATGTTGGTTACTTGTGTGGGGATGAAGACAACATTAGGTGAGCTGATGGGCCAAGTTAGATGTACACCTAACCAGGACCAATTACCAGCTCAACTTGATAACTTGAGCACAAGGTTGCAGAATGTTGGGCTCTTGATCACAATTTTCATGACTGTAGTGCTTATCATTCGTTCCGTGGTTCTAAAGGTTAATGAGTACCCTGGCCTCCAAGCTGATCAGTTCAAGGCTAAAACAACTGCAAGCGAGGAGCTAATCAATGCCATTGGCAGATTTTTCATGAAATCAAGTAGGCAGATTAGTGTGTTGGCGACGTATCTGCCTTTATTGATTGTAGGCTTAGCGGAAGGAGTACCTTTTTCAGTTGTACTTGCCATCAATTATTGGACCAAGACCACGCTGTCTGGGAAAGCCAGTGCTCAAAGAATCTTAGCTTGTTTCACCATGGGCTGTGTGTCAAATATCTGCATCAGTGATGGGCTAACTTTGAACCCTGTACTTGAAGTCGACGTGAGCTGTGATGGGGATCAAATAATCGATACAGAGAGTGTTGCTAGTATTGACAGACACGTTCGTGAAGCTTTATACAAAGGAATTAGCACACCACTTTTGATGCCGTCAAGCCCTTGTAGCTCAACAGAACACCGGGCCCTGCTACCGTGGGCCAAATCTAATTTGGGTATGGACAATGAGAATTTATTGCAAAATTGCACCATTGTGGGGAGCGAAGAGTTGAGCATCGATGAGGAGGCTAGTGGAGTTCTGATGAAGGACAGTGAAACTGGAGATATGTGCTTTTATTGGAATGGACCTGCAACAACAATATTGCCCATGTGTTCCGAATACTACGACAGCAAGGGGACAACAAAGCTCATGGATGAACAGAAGAAACGTGATTTTAAAGAAATTATGGGGAACATGCAGTCTAAACATCTCAAGACCATTGCATTTGCGTACAAGAAGATTGATGCTCCACTGCCTGAAGAAGGCAACAACTTAATATTGATAGGACTGCTAGGTGTGAAGTGTTGCACTGAGACAATGGAAGCACTGGAAGTTCTACAAAAAGCGGGAGTGGACATCATCCAGTTCCCAAAAGATAAAGAGTCAGTACTGTCGGAAGGTGAAAGTCCTGTTTATGGAATGGGAAGACCTGCTGACAAGCTCCACACACTACAACGTCTGAAGGAAGAGGGCCATGTTGTAGCAATGGTTGGGGCTAGAACAAATGAGACTCCAGCTCTAAAAGAAGCCGATGTGGGGATTGCAATAGGGACTTGCATTAGTGAAATGGCAAGAGAGGGTTCTGATCTCATCATCCCCGATTGCAGTCTCAGTTTCTTGGTCAACATTATTCGGATCGGAAGATGCATTCACTACAACATTCAGAAGTACATCCAAGTTGAGCTCACCATGAATATATCATTGCTTGTGACAATCTCGATCACTACAATGTCATTGGGACACGGCACAGTTGGGGCAATCGAAGTAGTGTGGGCAAATATGGTTGTGACACTATGTGGGCTTGCGCTACTGGTTGAGCCGCCAACAGAGGAGCAGATGGAACAGCCGCCGGTAAAACAAACTGAATCTCTCATTAGCAAGACAATGTGGAGAAACATATTACTTCAAGCTTTATATCAGGCTGCCATCTTGGTCACCTTTCGGTTCAAATATAAAGCTTTCCCAAGCATCAACGAGAAGATTATGGAGTCCATAGTTTTCAGCAGTTATGTTCTTTGTCAGGTATTTAATCAAGTCAGCTCAAGGGAGCTAGAGAAGAAAAATGTGTTCAGAGGTATACTTCGAACTCGATGGTTATGGGTGGCTGTGGGGGTTAGTATCACGATGCAAGTGGCTTTTGTTGAGATTGCACACAAGGTTGCTGGCAATGCAAGGTTGAATTGGGCACAATGGGGTGTCTGCCTTTTAGTAGGGATGGTTTCCTGGCCAATCGATTTGGCTGGGAAATGCGTATGGGGTGTCATCATGAAGATTAGAATACTTGAATCTGATATTGGATCCACCAGTATGGGGTCCTCTGCTGTCTCAGAATCAGCTTCTAATCAAGAGCTCCTAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase Hydrolase

Protein Analysis

1026

Amino Acids

112.27

Weight (kDa)

5.29

Isoelectric Point (pI)

39.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	256 - 352	1.5e-09	P-type ATPase actuator domain
Cation_ATPase	PF13246	549 - 673	7.2e-08	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	826 - 989	2.7e-34	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002542)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g00630 FvH4_3g13880
prunus_persica	Prupe.4G007300_v2.0.a1 Prupe.4G007300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0000841 RchiOBHm_Chr5g0000871
rosa_laevigata	RLG00000030901 RLG00000030902 RLG00000030903 RLG00000032693 RLG00000032697
rosa_multiflora	Rmu_co8361431.1_g000001 Rmu_sc0000255.1_g000013 Rmu_sc0000255.1_g000017 Rmu_sc0001979.1_g000005 Rmu_sc0002085.1_g000003 Rmu_sc0002085.1_g000004 Rmu_sc0010050.1_g000003 Rmu_sc0010050.1_g000006 Rmu_sc0019378.1_g000001 Rmu_sc0034511.1_g000001
rosa_roxburghii	Rroxscaffold_1G00056260 Rroxscaffold_1G00075400
rosa_rugosa	Rorug04G0387000 Rorug04G0387100 Rorug04G0387200 Rorug05G0076900
rosa_samantha	Rh5AG006900 Rh5AG007000 Rh5AG163100 Rh5BG009000 Rh5BG009100 Rh5BG162300 Rh5CG007600 Rh5CG007700 Rh5CG177100 Rh5DG007600 Rh5DG163600
rosa_wichuraiana	Rw5G000710 Rw5G000730 Rw5G014710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1396
Acc36I	ACCTGC	3 cut(s)	810, 1816, 2158
AccB7I	CCANNNNNTGG	1 cut(s)	665
AccI	GTMKAC	2 cut(s)	1503, 2797
AciI	CCGC	8 cut(s)	72, 480, 749, 787, 1340, 2066, 2540, 2567
AclWI	GGATC	6 cut(s)	364, 868, 1530, 2363, 3012, 3025
AcoI	YGGCCR	1 cut(s)	2945
AcsI	RAATTY	2 cut(s)	929, 1690
AcuI	CTGAAG	2 cut(s)	1998, 2201
AdeI	CACNNNGTG	1 cut(s)	2516
AfiI	CCNNNNNNNGG	8 cut(s)	665, 755, 878, 1716, 1982, 2135, 2256, 3030
AflIII	ACRYGT	1 cut(s)	1564
AgsI	TTSAA	9 cut(s)	94, 677, 1222, 1486, 1499, 2627, 2668, 2899, 3005
AhlI	ACTAGT	2 cut(s)	83, 764
AjiI	CACGTC	1 cut(s)	1507
AjnI	CCWGG	6 cut(s)	347, 487, 832, 1071, 1198, 2942
AjuI	GAANNNNNNNTTGG	2 cut(s)	2997, 3029
AleI	CACNNNNGTG	2 cut(s)	1569, 1712
Alw21I	GWGCWC	4 cut(s)	1109, 1417, 2406, 3072
Alw26I	GTCTC	7 cut(s)	92, 155, 535, 2027, 2222, 2333, 3049
AlwI	GGATC	6 cut(s)	364, 868, 1530, 2363, 3012, 3025
AlwNI	CAGNNNCTG	1 cut(s)	121
Ama87I	CYCGRG	1 cut(s)	755
AoxI	GGCC	7 cut(s)	655, 1048, 1201, 1644, 1659, 2191, 2945
ApaI	GGGCCC	1 cut(s)	1648
ApeKI	GCWGC	1 cut(s)	2642
ApoI	RAATTY	2 cut(s)	929, 1690
Asp700I	GAANNNNTTC	1 cut(s)	410
AspLEI	GCGC	1 cut(s)	2527
AsuC2I	CCSGG	3 cut(s)	756, 757, 1643
AsuII	TTCGAA	1 cut(s)	2803
AvaI	CYCGRG	1 cut(s)	755
AvaII	GGWCC	5 cut(s)	784, 1075, 1383, 1805, 3033
AxyI	CCTNAGG	1 cut(s)	237
BaeGI	GKGCMC	2 cut(s)	1648, 2908
BalI	TGGCCA	1 cut(s)	2947
BamHI	GGATCC	1 cut(s)	3017
BanII	GRGCYC	4 cut(s)	1131, 1648, 2406, 3072
BbsI	GAAGAC	2 cut(s)	1031, 2152
Bbv12I	GWGCWC	4 cut(s)	1109, 1417, 2406, 3072
BbvI	GCAGC	1 cut(s)	2629
BceAI	ACGGC	2 cut(s)	1597, 2479
BcgI	CGANNNNNNTGC	2 cut(s)	1297, 1331
BciT130I	CCWGG	6 cut(s)	349, 489, 834, 1073, 1200, 2944
BciVI	GTATCC	1 cut(s)	653
BclI	TGATCA	3 cut(s)	123, 1134, 1213
BcnI	CCSGG	3 cut(s)	756, 757, 1643
BcoDI	GTCTC	7 cut(s)	92, 155, 535, 2027, 2222, 2333, 3049
BcuI	ACTAGT	2 cut(s)	83, 764
BfaI	CTAG	9 cut(s)	84, 174, 765, 1551, 1749, 2012, 2217, 2771, 3074
BfmI	CTRYAG	1 cut(s)	1152
BfuAI	ACCTGC	3 cut(s)	810, 1816, 2158
BfuI	GTATCC	1 cut(s)	653
BisI	GCNGC	5 cut(s)	73, 749, 2540, 2567, 2643
BlpI	GCTNAGC	1 cut(s)	1336
BlsI	GCNGC	5 cut(s)	74, 750, 2541, 2568, 2644
BmcAI	AGTACT	1 cut(s)	2109
Bme1390I	CCNGG	9 cut(s)	349, 489, 756, 757, 834, 1073, 1200, 1643, 2944
Bme18I	GGWCC	5 cut(s)	784, 1075, 1383, 1805, 3033
BmeT110I	CYCGRG	1 cut(s)	755
BmgBI	CACGTC	1 cut(s)	1507
BmiI	GGNNCC	5 cut(s)	45, 546, 1646, 3019, 3034
BmrFI	CCNGG	9 cut(s)	349, 489, 756, 757, 834, 1073, 1200, 1643, 2944
BoxI	GACNNNNGTC	2 cut(s)	167, 951
BpiI	GAAGAC	2 cut(s)	1031, 2152
BpmI	CTGGAG	2 cut(s)	1800, 2217
Bpu1102I	GCTNAGC	1 cut(s)	1336
Bpu14I	TTCGAA	1 cut(s)	2803
BpuEI	CTTGAG	4 cut(s)	47, 1123, 1916, 2746
BpuMI	CCSGG	3 cut(s)	756, 757, 1643
Bsa29I	ATCGAT	3 cut(s)	1533, 1737, 2952
BsaBI	GATNNNNATC	1 cut(s)	1521
BsaJI	CCNNGG	6 cut(s)	487, 755, 1173, 1198, 1440, 1655
BsaXI	ACNNNNNCTCC	3 cut(s)	28, 529, 559
Bsc4I	CCNNNNNNNGG	8 cut(s)	665, 755, 878, 1716, 1982, 2135, 2256, 3030
Bse118I	RCCGGY	1 cut(s)	2569
Bse1I	ACTGG	9 cut(s)	115, 201, 901, 1410, 1783, 2052, 2083, 2535, 3024
Bse21I	CCTNAGG	1 cut(s)	237
Bse3DI	GCAATG	4 cut(s)	1938, 2211, 2420, 2893
Bse8I	GATNNNNATC	1 cut(s)	1521
BseBI	CCWGG	6 cut(s)	349, 489, 834, 1073, 1200, 2944
BseCI	ATCGAT	3 cut(s)	1533, 1737, 2952
BseDI	CCNNGG	6 cut(s)	487, 755, 1173, 1198, 1440, 1655
BseJI	GATNNNNATC	1 cut(s)	1521
BseLI	CCNNNNNNNGG	8 cut(s)	665, 755, 878, 1716, 1982, 2135, 2256, 3030
BseMI	GCAATG	4 cut(s)	1938, 2211, 2420, 2893
BseMII	CTCAG	6 cut(s)	251, 475, 756, 2022, 2344, 3060
BseNI	ACTGG	9 cut(s)	115, 201, 901, 1410, 1783, 2052, 2083, 2535, 3024
BseRI	GAGGAG	5 cut(s)	173, 807, 1259, 1757, 2564
BseSI	GKGCMC	2 cut(s)	1648, 2908
BseXI	GCAGC	1 cut(s)	2629
BseYI	CCCAGC	1 cut(s)	2960
BsgI	GTGCAG	1 cut(s)	543
BshFI	GGCC	7 cut(s)	657, 1050, 1203, 1646, 1661, 2193, 2947
BshVI	ATCGAT	3 cut(s)	1533, 1737, 2952
BsiHKAI	GWGCWC	4 cut(s)	1109, 1417, 2406, 3072
BsiHKCI	CYCGRG	1 cut(s)	755
BsiSI	CCGG	3 cut(s)	756, 1642, 2570
BslFI	GGGAC	3 cut(s)	1870, 2284, 2472
BslI	CCNNNNNNNGG	8 cut(s)	665, 755, 878, 1716, 1982, 2135, 2256, 3030
BsmAI	GTCTC	7 cut(s)	92, 155, 535, 2027, 2222, 2333, 3049
BsmFI	GGGAC	3 cut(s)	1870, 2284, 2472
BsmI	GAATGC	1 cut(s)	2367
BsnI	GGCC	7 cut(s)	657, 1050, 1203, 1646, 1661, 2193, 2947
BsoBI	CYCGRG	1 cut(s)	755
Bsp119I	TTCGAA	1 cut(s)	2803
Bsp120I	GGGCCC	1 cut(s)	1644
Bsp1286I	GDGCHC	7 cut(s)	1109, 1131, 1417, 1648, 2406, 2908, 3072
Bsp1407I	TGTACA	1 cut(s)	1061
Bsp1720I	GCTNAGC	1 cut(s)	1336
Bsp19I	CCATGG	1 cut(s)	1440
BspACI	CCGC	8 cut(s)	72, 480, 749, 787, 1340, 2066, 2540, 2567
BspANI	GGCC	7 cut(s)	657, 1050, 1203, 1646, 1661, 2193, 2947
BspCNI	CTCAG	6 cut(s)	250, 474, 755, 2023, 2343, 3059
BspDI	ATCGAT	3 cut(s)	1533, 1737, 2952
BspHI	TCATGA	4 cut(s)	508, 1146, 1275, 2985
BspLI	GGNNCC	5 cut(s)	45, 546, 1646, 3019, 3034
BspMI	ACCTGC	3 cut(s)	810, 1816, 2158
BspPI	GGATC	6 cut(s)	364, 868, 1530, 2363, 3012, 3025
BspT104I	TTCGAA	1 cut(s)	2803
BsrDI	GCAATG	4 cut(s)	1938, 2211, 2420, 2893
BsrFI	RCCGGY	1 cut(s)	2569
BsrGI	TGTACA	1 cut(s)	1061
BsrI	ACTGG	9 cut(s)	115, 201, 901, 1410, 1783, 2052, 2083, 2535, 3024
BssAI	RCCGGY	1 cut(s)	2569
BssECI	CCNNGG	6 cut(s)	487, 755, 1173, 1198, 1440, 1655
BssNAI	GTATAC	1 cut(s)	2798
BssT1I	CCWWGG	1 cut(s)	1440
Bst1107I	GTATAC	1 cut(s)	2798
Bst2UI	CCWGG	6 cut(s)	349, 489, 834, 1073, 1200, 2944
Bst6I	CTCTTC	3 cut(s)	614, 1719, 2181
BstAUI	TGTACA	1 cut(s)	1061
BstBI	TTCGAA	1 cut(s)	2803
BstC8I	GCNNGC	5 cut(s)	77, 378, 1240, 2523, 2884
BstDSI	CCRYGG	3 cut(s)	1173, 1440, 1655
BstEII	GGTNACC	1 cut(s)	2653
BstENI	CCTNNNNNAGG	1 cut(s)	1980
BstHHI	GCGC	1 cut(s)	2527
BstMAI	GTCTC	7 cut(s)	92, 155, 535, 2027, 2222, 2333, 3049
BstMWI	GCNNNNNNNGC	3 cut(s)	362, 1626, 2888
BstNI	CCWGG	6 cut(s)	349, 489, 834, 1073, 1200, 2944
BstNSI	RCATGY	3 cut(s)	380, 1003, 1918
BstPAI	GACNNNNGTC	2 cut(s)	167, 951
BstPI	GGTNACC	1 cut(s)	2653
BstSCI	CCNGG	9 cut(s)	347, 487, 754, 755, 832, 1071, 1198, 1641, 2942
BstSFI	CTRYAG	1 cut(s)	1152
BstSLI	GKGCMC	2 cut(s)	1648, 2908
BstV1I	GCAGC	1 cut(s)	2629
BstV2I	GAAGAC	2 cut(s)	1031, 2152
BstX2I	RGATCY	3 cut(s)	369, 860, 3017
BstXI	CCANNNNNNTGG	1 cut(s)	3028
BstYI	RGATCY	3 cut(s)	369, 860, 3017
BstZ17I	GTATAC	1 cut(s)	2798
Bsu15I	ATCGAT	3 cut(s)	1533, 1737, 2952
Bsu36I	CCTNAGG	1 cut(s)	237
BsuI	GTATCC	1 cut(s)	653
BsuRI	GGCC	7 cut(s)	657, 1050, 1203, 1646, 1661, 2193, 2947
BsuTUI	ATCGAT	3 cut(s)	1533, 1737, 2952
BtgI	CCRYGG	3 cut(s)	1173, 1440, 1655
BtrI	CACGTC	1 cut(s)	1507
BtsI	GCAGTG	2 cut(s)	526, 1970
BtsIMutI	CAGTG	8 cut(s)	526, 750, 1417, 1474, 1777, 1970, 2028, 2045
BveI	ACCTGC	3 cut(s)	810, 1816, 2158
Cac8I	GCNNGC	5 cut(s)	77, 378, 1240, 2523, 2884
CaiI	CAGNNNCTG	1 cut(s)	121
CciI	TCATGA	4 cut(s)	508, 1146, 1275, 2985
CfoI	GCGC	1 cut(s)	2527
Cfr10I	RCCGGY	1 cut(s)	2569
Cfr9I	CCCGGG	1 cut(s)	755
ClaI	ATCGAT	3 cut(s)	1533, 1737, 2952
CseI	GACGC	1 cut(s)	525
DraI	TTTAAA	2 cut(s)	565, 1897
DraIII	CACNNNGTG	1 cut(s)	2516
DrdI	GACNNNNNNGTC	1 cut(s)	1396
DseDI	GACNNNNNNGTC	1 cut(s)	1396
EaeI	YGGCCR	1 cut(s)	2945
Eam1104I	CTCTTC	3 cut(s)	614, 1719, 2181
EarI	CTCTTC	3 cut(s)	614, 1719, 2181
Ecl136II	GAGCTC	2 cut(s)	2404, 3070
Eco130I	CCWWGG	1 cut(s)	1440
Eco24I	GRGCYC	4 cut(s)	1131, 1648, 2406, 3072
Eco47I	GGWCC	5 cut(s)	784, 1075, 1383, 1805, 3033
Eco53kI	GAGCTC	2 cut(s)	2404, 3070
Eco57I	CTGAAG	2 cut(s)	1998, 2201
Eco81I	CCTNAGG	1 cut(s)	237
Eco88I	CYCGRG	1 cut(s)	755
Eco91I	GGTNACC	1 cut(s)	2653
EcoICRI	GAGCTC	2 cut(s)	2404, 3070
EcoNI	CCTNNNNNAGG	1 cut(s)	1980
EcoO109I	RGGNCCY	2 cut(s)	1645, 3033
EcoO65I	GGTNACC	1 cut(s)	2653
EcoRII	CCWGG	6 cut(s)	347, 487, 832, 1071, 1198, 2942
EcoT14I	CCWWGG	1 cut(s)	1440
EcoT22I	ATGCAT	1 cut(s)	2369
EcoT38I	GRGCYC	4 cut(s)	1131, 1648, 2406, 3072
ErhI	CCWWGG	1 cut(s)	1440
FalI	AAGNNNNNCTT	2 cut(s)	9, 41
FaqI	GGGAC	3 cut(s)	1870, 2284, 2472
FauI	CCCGC	1 cut(s)	2059
FbaI	TGATCA	3 cut(s)	123, 1134, 1213
FblI	GTMKAC	2 cut(s)	1503, 2797
Fnu4HI	GCNGC	5 cut(s)	73, 749, 2540, 2567, 2643
FriOI	GRGCYC	4 cut(s)	1131, 1648, 2406, 3072
Fsp4HI	GCNGC	5 cut(s)	73, 749, 2540, 2567, 2643
FspBI	CTAG	9 cut(s)	84, 174, 765, 1551, 1749, 2012, 2217, 2771, 3074
GlaI	GCGC	1 cut(s)	2526
GluI	GCNGC	5 cut(s)	73, 749, 2540, 2567, 2643
GsaI	CCCAGC	1 cut(s)	2964
GsuI	CTGGAG	2 cut(s)	1800, 2217
HaeIII	GGCC	7 cut(s)	657, 1050, 1203, 1646, 1661, 2193, 2947
HapII	CCGG	3 cut(s)	756, 1642, 2570
HgaI	GACGC	1 cut(s)	525
HhaI	GCGC	1 cut(s)	2527
Hin6I	GCGC	1 cut(s)	2525
HinP1I	GCGC	1 cut(s)	2525
HincII	GTYRAC	3 cut(s)	630, 1504, 2344
HindII	GTYRAC	3 cut(s)	630, 1504, 2344
HindIII	AAGCTT	4 cut(s)	556, 1575, 2628, 2676
HpaII	CCGG	3 cut(s)	756, 1642, 2570
Hpy166II	GTNNAC	6 cut(s)	630, 1064, 1504, 2074, 2344, 2798
Hpy8I	GTNNAC	6 cut(s)	630, 1064, 1504, 2074, 2344, 2798
Hpy99I	CGWCG	4 cut(s)	519, 944, 1312, 1508
HpyCH4IV	ACGT	5 cut(s)	1310, 1506, 1566, 1888, 2176
HpyF10VI	GCNNNNNNNGC	3 cut(s)	362, 1626, 2888
HpySE526I	ACGT	5 cut(s)	1310, 1506, 1566, 1888, 2176
HspAI	GCGC	1 cut(s)	2525
Ksp22I	TGATCA	3 cut(s)	123, 1134, 1213
LmnI	GCTCC	9 cut(s)	550, 680, 1246, 1719, 1972, 2166, 2551, 2767, 3075
Lsp1109I	GCAGC	1 cut(s)	2629
MaeI	CTAG	9 cut(s)	84, 174, 765, 1551, 1749, 2012, 2217, 2771, 3074
MaeII	ACGT	5 cut(s)	1310, 1506, 1566, 1888, 2176
MaeIII	GTNAC	6 cut(s)	169, 836, 1006, 2428, 2506, 2653
MflI	RGATCY	3 cut(s)	369, 860, 3017
MhlI	GDGCHC	7 cut(s)	1109, 1131, 1417, 1648, 2406, 2908, 3072
MlsI	TGGCCA	1 cut(s)	2947
MluNI	TGGCCA	1 cut(s)	2947
MlyI	GAGTC	3 cut(s)	2111, 2224, 2717
MmeI	TCCRAC	6 cut(s)	572, 598, 667, 894, 959, 2094
Mox20I	TGGCCA	1 cut(s)	2947
Mph1103I	ATGCAT	1 cut(s)	2369
MroXI	GAANNNNTTC	1 cut(s)	410
MscI	TGGCCA	1 cut(s)	2947
MseI	TTAA	6 cut(s)	564, 780, 1188, 1896, 1994, 2748
MslI	CAYNNNNRTG	5 cut(s)	710, 1569, 1712, 2447, 3026
Msp20I	TGGCCA	1 cut(s)	2947
MspA1I	CMGCKG	1 cut(s)	480
MspI	CCGG	3 cut(s)	756, 1642, 2570
MspR9I	CCNGG	9 cut(s)	349, 489, 756, 757, 834, 1073, 1200, 1643, 2944
Mva1269I	GAATGC	1 cut(s)	2367
MvaI	CCWGG	6 cut(s)	349, 489, 834, 1073, 1200, 2944
MwoI	GCNNNNNNNGC	3 cut(s)	362, 1626, 2888
NciI	CCSGG	3 cut(s)	756, 757, 1643
NcoI	CCATGG	1 cut(s)	1440
NlaIV	GGNNCC	5 cut(s)	45, 546, 1646, 3019, 3034
NmuCI	GTSAC	5 cut(s)	169, 836, 2428, 2506, 2653
NsiI	ATGCAT	1 cut(s)	2369
NspI	RCATGY	3 cut(s)	380, 1003, 1918
NspV	TTCGAA	1 cut(s)	2803
OliI	CACNNNNGTG	2 cut(s)	1569, 1712
PaeI	GCATGC	1 cut(s)	380
PagI	TCATGA	4 cut(s)	508, 1146, 1275, 2985
PctI	GAATGC	1 cut(s)	2367
PdmI	GAANNNNTTC	1 cut(s)	410
PfeI	GAWTC	8 cut(s)	31, 319, 483, 902, 1422, 2585, 3005, 3050
PflMI	CCANNNNNTGG	1 cut(s)	665
PkrI	GCNGC	5 cut(s)	74, 750, 2541, 2568, 2644
PleI	GAGTC	3 cut(s)	2110, 2224, 2716
PpsI	GAGTC	3 cut(s)	2110, 2224, 2716
PpuMI	RGGWCCY	1 cut(s)	3033
PshAI	GACNNNNGTC	2 cut(s)	167, 951
Psp124BI	GAGCTC	2 cut(s)	2406, 3072
Psp5II	RGGWCCY	1 cut(s)	3033
Psp6I	CCWGG	6 cut(s)	347, 487, 832, 1071, 1198, 2942
PspEI	GGTNACC	1 cut(s)	2653
PspFI	CCCAGC	1 cut(s)	2960
PspGI	CCWGG	6 cut(s)	347, 487, 832, 1071, 1198, 2942
PspN4I	GGNNCC	5 cut(s)	45, 546, 1646, 3019, 3034
PspOMI	GGGCCC	1 cut(s)	1644
PspPPI	RGGWCCY	1 cut(s)	3033
PstNI	CAGNNNCTG	1 cut(s)	121
PsuI	RGATCY	3 cut(s)	369, 860, 3017
RseI	CAYNNNNRTG	5 cut(s)	710, 1569, 1712, 2447, 3026
SacI	GAGCTC	2 cut(s)	2406, 3072
SalI	GTCGAC	1 cut(s)	1502
SaqAI	TTAA	6 cut(s)	564, 780, 1188, 1896, 1994, 2748
SatI	GCNGC	5 cut(s)	73, 749, 2540, 2567, 2643
ScaI	AGTACT	1 cut(s)	2109
SchI	GAGTC	3 cut(s)	2111, 2224, 2717
ScrFI	CCNGG	9 cut(s)	349, 489, 756, 757, 834, 1073, 1200, 1643, 2944
SduI	GDGCHC	7 cut(s)	1109, 1131, 1417, 1648, 2406, 2908, 3072
SfcI	CTRYAG	1 cut(s)	1152
SfuI	TTCGAA	1 cut(s)	2803
SinI	GGWCC	5 cut(s)	784, 1075, 1383, 1805, 3033
SmaI	CCCGGG	1 cut(s)	757
SmiMI	CAYNNNNRTG	5 cut(s)	710, 1569, 1712, 2447, 3026
SmlI	CTYRAG	4 cut(s)	26, 1102, 1931, 2761
SmoI	CTYRAG	4 cut(s)	26, 1102, 1931, 2761
SpeI	ACTAGT	2 cut(s)	83, 764
SphI	GCATGC	1 cut(s)	380
SsiI	CCGC	8 cut(s)	72, 480, 749, 787, 1340, 2066, 2540, 2567
SspI	AATATT	3 cut(s)	385, 1821, 1998
SspMI	CTAG	9 cut(s)	84, 174, 765, 1551, 1749, 2012, 2217, 2771, 3074
SstI	GAGCTC	2 cut(s)	2406, 3072
StyD4I	CCNGG	9 cut(s)	347, 487, 754, 755, 832, 1071, 1198, 1641, 2942
StyI	CCWWGG	1 cut(s)	1440
TaiI	ACGT	5 cut(s)	1313, 1509, 1569, 1891, 2179
TatI	WGTACW	6 cut(s)	464, 1061, 1363, 1492, 2107, 2388
TauI	GCSGC	4 cut(s)	75, 751, 2542, 2569
TfiI	GAWTC	8 cut(s)	31, 319, 483, 902, 1422, 2585, 3005, 3050
Tru1I	TTAA	6 cut(s)	564, 780, 1188, 1896, 1994, 2748
Tru9I	TTAA	6 cut(s)	564, 780, 1188, 1896, 1994, 2748
TscAI	CASTG	8 cut(s)	526, 750, 1417, 1474, 1777, 1977, 2035, 2052
TseFI	GTSAC	5 cut(s)	169, 836, 2428, 2506, 2653
TseI	GCWGC	1 cut(s)	2642
Tsp45I	GTSAC	5 cut(s)	169, 836, 2428, 2506, 2653
TspGWI	ACGGA	1 cut(s)	1162
TspMI	CCCGGG	1 cut(s)	755
TspRI	CASTG	8 cut(s)	526, 750, 1417, 1474, 1777, 1977, 2035, 2052
Van91I	CCANNNNNTGG	1 cut(s)	665
VpaK11BI	GGWCC	5 cut(s)	784, 1075, 1383, 1805, 3033
XagI	CCTNNNNNAGG	1 cut(s)	1980
XapI	RAATTY	2 cut(s)	929, 1690
XceI	RCATGY	3 cut(s)	380, 1003, 1918
XcmI	CCANNNNNNNNNTGG	2 cut(s)	666, 1669
XmaI	CCCGGG	1 cut(s)	755
XmiI	GTMKAC	2 cut(s)	1503, 2797
XmnI	GAANNNNTTC	1 cut(s)	410
XspI	CTAG	9 cut(s)	84, 174, 765, 1551, 1749, 2012, 2217, 2771, 3074
ZrmI	AGTACT	1 cut(s)	2109
Zsp2I	ATGCAT	1 cut(s)	2369

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.