Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0002542

Physical Location & Seq

Genomic Coordinates Reverse (-)

506239 .. 509880

Gene Structure 3642 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G000730.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3213 bp

ATGCCAGACATCGCAAGCCCTGTGCACCAGTTCGACCTCGAGTCTCAGCATACAGTGTTGCTGAAAACCAGTAGTACTAACCCACCAATAGACCACTGGCGCTGGATTTATGTGATTCTCCAAGTGCGCAAACTCCTCATCTCATCCAGAGCCAACAAACCTAAAAACGGGTCTCAACCACCTCACCACCTCTCATTTGCTATTATCAGCACCAGCACTACTACTGGTGATGCAAGAGATGCGGACTTGCAACTGACATCAGGGACTACAGAAATCTTACCTGCCTCGCCTCAATTTTCTGGAGATGTCCATCAGATCGGGGAGGGTGATGGTAGTACTGCTGCTACTGATGCAAAACTCCAGCGTACAAATATTGCCAAGATAGTCACGGAGAAAGACTTTGAGTCCTTACAGAAGTTTGGATGCGTCCAAGGAATTGCAGAGGCACTTAACAGCGATTTACATAATGGAATCCCAGGCAATGAAGATGTGAGTTCTCGCCGCCCAGTTAGTGCCCTCTTAACCACACAGGCTCCTGTTCAAGGCTTCTTCAAACTGCTCCTGCAGTCCAGCAACAACTGCATCATTTTCCTTCTCTTGGTGGCTGCAGTGCTGTCGATTGGCTTTGGTATGAAGGAGGAAGGCCCAAGCACCGGTTGGTATGAAGGGGCCATCATAGCGTTTGTTCTCATCATACTCGTTGTTGCGCCTTCCATACGCGACTTTTGGCTTCAAAATCCACAGAGCCATGCAATGAGACAGACTGCAGAGGCCATTAAAATGGTGGTTGAGGTCATCAGAGGGGGATCCCGATGTGAAATACCCATATATGATGTTGTTTCAGGTGACATAGTGTGCTTGAAACGGGGATCTATAGTTCCCGGTGATGGTTTGTTTGTTTCTGGTGAGTTCTTGGTATTGGATGATGGTGCAGTGGCAACCATTTATGACAAGAAACCATTCTTGTTCTGTGGTTCAAAGGTGGTTGGTGGAAGTGGTCGCATGCTTGTAACGTCTGTGGGCATGGACACAGCATGGGGTGAGCTGATGAAAAAAGTAGCCAATACTCCTGACCGGGCTCATTTACCAGCTCAACTTGATAAGGTCAGCACAGGCACGCAGATTATAGGGATCTCAATCTCTATTCTCATCCTGGTAGTGTTGTTCTCCCGTTTCATGATTGAAAGGGAAGACAATCACTCCGGCCTCCCAGACCTTAAGGGGAAACCAACTGCAAGCAAGGAGATAATGAATTTAATTGAGAAAATTGTGATGAAACCAAGTGGTCGGATCAGTATCCTAACGACATCTCTGGCCATATTTCTCGTAGGAGTAGTGGAAGGAATACCTCTTTTTGTTACACTTGCCATAAGGTATTGGAATAAGAAGACTTTATCTGGAAAGGCCACTGCTCAAGGGACTTTGGCGTGTGTCACCATGGGCTCTATCACAACTATTTGCACTGACAAAACTGGCGTGCTGACCTTGAACACACTGGAAGTGGACAAGTGCTACATCGGGGATGAAGTCATAGAAACTGACCGTGTCACTGGAATAGACACACATGTTCGTGAAGCTTTGTGCAATGGAATCAGCACACCCCTTCTGATGCCATCAAACCCTCATTGTAGCTCGAGAGAGGACCCGCTCCTTTCCTGCTCTAACTTCAATTTGGGAATGCAATATGAGATTTTGAGGCAAAATTGCACCATCCTGGAGACCAAAGGATTGAGCACCAATGAGGAAGGCAGTGGAGTACTGATGAGAAGAAGCAGTACTGACAACGAAGGAGATATGCGCTTGCATTGGAAAGGACCTGCAACAACAATATTAGCTATGTGTTCGCACTACAATGACAACAAAGGGGAAACAAAGGTCATGGATGAACAAAGTACATTGGTTTTTAACCAAATTATAGAGGGCATGCAGTCCAATTCTCTCAAGACCATTGCATTTGCTTACAGACAGACTAAGGTTCCAATGCTAGAAGAAAACAGCTTAATCTTAATAGCATTGCTAGGTGTGAGGTATTCATGCTGCAGAGATATAGCGGAAGCTTGTCAAGAAGCAGGAGTCAACATCATACTGGTCTCAGAAGATAATGTTTCGGACCTGGAAGACATTGCTAGTGAGTGTGGAATACTTCCGAACCCAAACAGATTGGTAGTTGAAGGTAAAAGTTTTCGAAATAGTGCCCAAGAAAACAGGATGGACATGGCAGATAAAATATGTGTGATGGGAAGCTCCATTCCTTCTGACAGGCTCCTTTTGGTGCAATGTCTGAAAGAAAAAGGCCATGTCGTAGCAATGGTTGGAGTCGGAACAAATGACGCTCCAACTCTGAAAGAAGCTGATGTGGGCATTGCAATGGGGACTTGGAGTAATGGAGTGGCAAGAGAGAGTTCTGACATCATCATCTGGGACGGAAATGTTAGTTTCTTGGTCACCATTATTGGGTGTGGAAGAAGCATATACTATAACATTCAGAAGTACATTCAACTTGAGCTCACCATGAATATATCATGGCTGTTGATAACATCCGCTACCACAATGTGCTTAGGACAAAGTGCAATTGCAGCCATACAATTGGTCTGGGCAAACATGATTGTGACTCTTCTAGGTGGGCTTGCTCTATTGATGGAGCCTCCAACGGAGGAACTAATGAAAAAGGCACCGGTCAAGCAAAATGAATCTCTCATTAGCAAGGCAATGTGGAGAAACATAGTCAGTCAAGCTTTATATCAGACTACCATCTTGGTCGCCTTTCAATTCAAAGGACCAACTATCCCAGGCATCAGCAAGAAGGTCAGTGAGTCCATTGTTTTCAACAGTTTTGTTCTCTGTCAGGTATTCAACCAAGTGAATTCAAGGGAACTCGAGAAGAAAAACGTATTTAGGGGCATATTTCACAATCTGTTGTTTTGGGTGGCTGTGGGTGCCATTCTTATCCTGCAGGCGGGTTTTGTTGAGATTGCACACATTCTGGTTGGCAATGCAAGGTTGAATTGGGCAGAGTGGGGTATCTGTCTTCTAATTGGGATGGTTTCATGGGCAATTGATTTGGTTGTTAAGTTTGCAACCAGTTTCATACTTAGCTCTGCAGGATCCATTAGTATGCCTATGACACCCTCTAATGTTTCAGAATCCGCCCTTAATCTAGAGCTCCCACTCATTAATGAAATTCCACATGAATCCCATCTTAGGCTGTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase Hydrolase

Protein Analysis

1070

Amino Acids

116.61

Weight (kDa)

5.73

Isoelectric Point (pI)

35.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	262 - 353	3e-10	P-type ATPase actuator domain
Cation_ATPase	PF13246	553 - 658	1.9e-09	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	856 - 1025	1.8e-36	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002542)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g00630 FvH4_3g13880
prunus_persica	Prupe.4G007300_v2.0.a1 Prupe.4G007300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0000841 RchiOBHm_Chr5g0000871
rosa_laevigata	RLG00000030901 RLG00000030902 RLG00000030903 RLG00000032693 RLG00000032697
rosa_multiflora	Rmu_co8361431.1_g000001 Rmu_sc0000255.1_g000013 Rmu_sc0000255.1_g000017 Rmu_sc0001979.1_g000005 Rmu_sc0002085.1_g000003 Rmu_sc0002085.1_g000004 Rmu_sc0010050.1_g000003 Rmu_sc0010050.1_g000006 Rmu_sc0019378.1_g000001 Rmu_sc0034511.1_g000001
rosa_roxburghii	Rroxscaffold_1G00056260 Rroxscaffold_1G00075400
rosa_rugosa	Rorug04G0387000 Rorug04G0387100 Rorug04G0387200 Rorug05G0076900
rosa_samantha	Rh5AG006900 Rh5AG007000 Rh5AG163100 Rh5BG009000 Rh5BG009100 Rh5BG162300 Rh5CG007600 Rh5CG007700 Rh5CG177100 Rh5DG007600 Rh5DG163600
rosa_wichuraiana	Rw5G000710 Rw5G000730 Rw5G014710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	128
Acc36I	ACCTGC	2 cut(s)	289, 1825
AccB1I	GGYRCC	2 cut(s)	2671, 2936
AccBSI	CCGCTC	1 cut(s)	1648
AccII	CGCG	1 cut(s)	720
AciI	CCGC	7 cut(s)	242, 502, 1646, 2051, 2541, 2957, 3147
AclWI	GGATC	7 cut(s)	801, 814, 877, 1139, 1298, 3099, 3112
AcoI	YGGCCR	1 cut(s)	1314
AcsI	RAATTY	3 cut(s)	1252, 2863, 3180
AdeI	CACNNNGTG	1 cut(s)	2553
AfaI	GTAC	7 cut(s)	76, 337, 367, 1758, 1777, 1894, 2492
AfiI	CCNNNNNNNGG	8 cut(s)	167, 542, 598, 653, 887, 2454, 2956, 3201
AflII	CTTAAG	1 cut(s)	1217
AflIII	ACRYGT	1 cut(s)	1564
AgeI	ACCGGT	2 cut(s)	653, 2674
AhdI	GACNNNNNGTC	2 cut(s)	40, 403
AjnI	CCWGG	5 cut(s)	475, 1152, 1713, 2112, 2788
AloI	GAACNNNNNNTCC	2 cut(s)	861, 893
Alw21I	GWGCWC	4 cut(s)	27, 1736, 2508, 3165
Alw26I	GTCTC	5 cut(s)	48, 177, 751, 1712, 2095
Alw44I	GTGCAC	1 cut(s)	23
AlwI	GGATC	7 cut(s)	801, 814, 877, 1139, 1298, 3099, 3112
Ama87I	CYCGRG	3 cut(s)	38, 1633, 2876
AoxI	GGCC	7 cut(s)	643, 669, 771, 1204, 1314, 1404, 2293
ApaLI	GTGCAC	1 cut(s)	23
ApeKI	GCWGC	4 cut(s)	341, 605, 2037, 2576
ApoI	RAATTY	3 cut(s)	1252, 2863, 3180
AseI	ATTAAT	1 cut(s)	3174
AsiGI	ACCGGT	2 cut(s)	653, 2674
Asp700I	GAANNNNTTC	1 cut(s)	959
AspLEI	GCGC	4 cut(s)	102, 129, 709, 1800
AspS9I	GGNCC	6 cut(s)	644, 669, 1642, 1814, 2110, 2777
AsuC2I	CCSGG	2 cut(s)	882, 1076
AsuII	TTCGAA	1 cut(s)	2185
AvaI	CYCGRG	3 cut(s)	38, 1633, 2876
AvaII	GGWCC	4 cut(s)	1642, 1814, 2110, 2777
BaeGI	GKGCMC	3 cut(s)	27, 517, 2197
BalI	TGGCCA	1 cut(s)	1316
BamHI	GGATCC	2 cut(s)	806, 3104
BanI	GGYRCC	2 cut(s)	2671, 2936
BanII	GRGCYC	4 cut(s)	1081, 1445, 2508, 3165
BarI	GAAGNNNNNNTAC	2 cut(s)	1759, 1791
BbsI	GAAGAC	4 cut(s)	1197, 1394, 2124, 3020
Bbv12I	GWGCWC	4 cut(s)	27, 1736, 2508, 3165
BbvI	GCAGC	4 cut(s)	328, 592, 2024, 2588
BcgI	CGANNNNNNTGC	2 cut(s)	597, 631
BciT130I	CCWGG	5 cut(s)	477, 1154, 1715, 2114, 2790
BciVI	GTATCC	1 cut(s)	1307
BcnI	CCSGG	2 cut(s)	882, 1076
BcoDI	GTCTC	5 cut(s)	48, 177, 751, 1712, 2095
BfaI	CTAG	5 cut(s)	1985, 2018, 2127, 2618, 3158
BfmI	CTRYAG	8 cut(s)	267, 563, 606, 765, 873, 2038, 2951, 3099
BfoI	RGCGCY	1 cut(s)	103
BfrI	CTTAAG	1 cut(s)	1217
BfuAI	ACCTGC	2 cut(s)	289, 1825
BfuI	GTATCC	1 cut(s)	1307
BisI	GCNGC	5 cut(s)	342, 502, 606, 2038, 2577
BlsI	GCNGC	5 cut(s)	343, 503, 607, 2039, 2578
BmcAI	AGTACT	4 cut(s)	76, 337, 1758, 1777
Bme1390I	CCNGG	7 cut(s)	477, 882, 1076, 1154, 1715, 2114, 2790
Bme18I	GGWCC	4 cut(s)	1642, 1814, 2110, 2777
BmeRI	GACNNNNNGTC	2 cut(s)	40, 403
BmeT110I	CYCGRG	3 cut(s)	38, 1633, 2876
BmgT120I	GGNCC	6 cut(s)	644, 669, 1642, 1814, 2110, 2777
BmrFI	CCNGG	7 cut(s)	477, 882, 1076, 1154, 1715, 2114, 2790
BmrI	ACTGGG	1 cut(s)	500
BmsI	GCATC	7 cut(s)	220, 229, 340, 413, 591, 1599, 2802
BmuI	ACTGGG	1 cut(s)	500
BpiI	GAAGAC	4 cut(s)	1197, 1394, 2124, 3020
BpmI	CTGGAG	3 cut(s)	321, 344, 1736
Bpu10I	CCTNAGC	1 cut(s)	2557
Bpu14I	TTCGAA	1 cut(s)	2185
BpuEI	CTTGAG	3 cut(s)	1398, 1925, 2522
BpuMI	CCSGG	2 cut(s)	882, 1076
BsaBI	GATNNNNATC	3 cut(s)	309, 1136, 1295
BsaI	GGTCTC	3 cut(s)	177, 1712, 2095
BsaJI	CCNNGG	4 cut(s)	430, 475, 1437, 2788
BsaWI	WCCGGW	2 cut(s)	653, 2674
BsaXI	ACNNNNNCTCC	4 cut(s)	517, 547, 1184, 1214
Bsc4I	CCNNNNNNNGG	8 cut(s)	167, 542, 598, 653, 887, 2454, 2956, 3201
Bse118I	RCCGGY	2 cut(s)	653, 2674
Bse8I	GATNNNNATC	3 cut(s)	309, 1136, 1295
BseBI	CCWGG	5 cut(s)	477, 1154, 1715, 2114, 2790
BseDI	CCNNGG	4 cut(s)	430, 475, 1437, 2788
BseJI	GATNNNNATC	3 cut(s)	309, 1136, 1295
BseLI	CCNNNNNNNGG	8 cut(s)	167, 542, 598, 653, 887, 2454, 2956, 3201
BseMII	CTCAG	2 cut(s)	59, 2106
BseRI	GAGGAG	1 cut(s)	125
BseSI	GKGCMC	3 cut(s)	27, 517, 2197
BseXI	GCAGC	4 cut(s)	328, 592, 2024, 2588
BsgI	GTGCAG	1 cut(s)	951
Bsh1236I	CGCG	1 cut(s)	720
BshFI	GGCC	7 cut(s)	645, 671, 773, 1206, 1316, 1406, 2295
BshNI	GGYRCC	2 cut(s)	2671, 2936
BshTI	ACCGGT	2 cut(s)	653, 2674
BsiHKAI	GWGCWC	4 cut(s)	27, 1736, 2508, 3165
BsiHKCI	CYCGRG	3 cut(s)	38, 1633, 2876
BsiSI	CCGG	5 cut(s)	654, 882, 1075, 1203, 2675
BslFI	GGGAC	4 cut(s)	277, 1432, 2386, 2435
BslI	CCNNNNNNNGG	8 cut(s)	167, 542, 598, 653, 887, 2454, 2956, 3201
BsmAI	GTCTC	5 cut(s)	48, 177, 751, 1712, 2095
BsmFI	GGGAC	4 cut(s)	277, 1432, 2386, 2435
BsmI	GAATGC	1 cut(s)	1683
BsnI	GGCC	7 cut(s)	645, 671, 773, 1206, 1316, 1406, 2295
Bso31I	GGTCTC	3 cut(s)	177, 1712, 2095
BsoBI	CYCGRG	3 cut(s)	38, 1633, 2876
Bsp119I	TTCGAA	1 cut(s)	2185
Bsp1286I	GDGCHC	8 cut(s)	27, 517, 1081, 1445, 1736, 2197, 2508, 3165
Bsp143I	GATC	6 cut(s)	315, 806, 869, 1131, 1290, 3104
Bsp19I	CCATGG	1 cut(s)	1437
BspACI	CCGC	7 cut(s)	242, 502, 1646, 2051, 2541, 2957, 3147
BspANI	GGCC	7 cut(s)	645, 671, 773, 1206, 1316, 1406, 2295
BspCNI	CTCAG	2 cut(s)	58, 2105
BspFNI	CGCG	1 cut(s)	720
BspHI	TCATGA	1 cut(s)	1176
BspMAI	CTGCAG	6 cut(s)	567, 610, 769, 2042, 2955, 3103
BspMI	ACCTGC	2 cut(s)	289, 1825
BspPI	GGATC	7 cut(s)	801, 814, 877, 1139, 1298, 3099, 3112
BspT104I	TTCGAA	1 cut(s)	2185
BspT107I	GGYRCC	2 cut(s)	2671, 2936
BspTI	CTTAAG	1 cut(s)	1217
BspTNI	GGTCTC	3 cut(s)	177, 1712, 2095
BsrBI	CCGCTC	1 cut(s)	1648
BsrFI	RCCGGY	2 cut(s)	653, 2674
BssAI	RCCGGY	2 cut(s)	653, 2674
BssECI	CCNNGG	4 cut(s)	430, 475, 1437, 2788
BssMI	GATC	6 cut(s)	315, 806, 869, 1131, 1290, 3104
BssT1I	CCWWGG	2 cut(s)	430, 1437
Bst2UI	CCWGG	5 cut(s)	477, 1154, 1715, 2114, 2790
Bst4CI	ACNGT	3 cut(s)	55, 1544, 2831
Bst6I	CTCTTC	1 cut(s)	2619
BstAFI	CTTAAG	1 cut(s)	1217
BstAPI	GCANNNNNTGC	2 cut(s)	239, 579
BstBI	TTCGAA	1 cut(s)	2185
BstC8I	GCNNGC	9 cut(s)	16, 1004, 1118, 1237, 1478, 1802, 1925, 2628, 2955
BstDEI	CTNAG	6 cut(s)	45, 1971, 2092, 2557, 3092, 3200
BstDSI	CCRYGG	1 cut(s)	1437
BstEII	GGTNACC	1 cut(s)	2443
BstENI	CCTNNNNNAGG	1 cut(s)	540
BstFNI	CGCG	1 cut(s)	720
BstH2I	RGCGCY	1 cut(s)	103
BstHHI	GCGC	4 cut(s)	102, 129, 709, 1800
BstKTI	GATC	6 cut(s)	318, 809, 872, 1134, 1293, 3107
BstMAI	GTCTC	5 cut(s)	48, 177, 751, 1712, 2095
BstMBI	GATC	6 cut(s)	315, 806, 869, 1131, 1290, 3104
BstMWI	GCNNNNNNNGC	6 cut(s)	239, 350, 579, 677, 2576, 2936
BstNI	CCWGG	5 cut(s)	477, 1154, 1715, 2114, 2790
BstNSI	RCATGY	3 cut(s)	1006, 1568, 1927
BstPI	GGTNACC	1 cut(s)	2443
BstSCI	CCNGG	7 cut(s)	475, 880, 1074, 1152, 1713, 2112, 2788
BstSFI	CTRYAG	8 cut(s)	267, 563, 606, 765, 873, 2038, 2951, 3099
BstSLI	GKGCMC	3 cut(s)	27, 517, 2197
BstUI	CGCG	1 cut(s)	720
BstV1I	GCAGC	4 cut(s)	328, 592, 2024, 2588
BstV2I	GAAGAC	4 cut(s)	1197, 1394, 2124, 3020
BstX2I	RGATCY	4 cut(s)	806, 869, 1131, 3104
BstXI	CCANNNNNNTGG	2 cut(s)	781, 2587
BstYI	RGATCY	4 cut(s)	806, 869, 1131, 3104
BsuI	GTATCC	1 cut(s)	1307
BsuRI	GGCC	7 cut(s)	645, 671, 773, 1206, 1316, 1406, 2295
BtgI	CCRYGG	1 cut(s)	1437
BtsI	GCAGTG	4 cut(s)	615, 939, 1407, 1756
BveI	ACCTGC	2 cut(s)	289, 1825
Cac8I	GCNNGC	9 cut(s)	16, 1004, 1118, 1237, 1478, 1802, 1925, 2628, 2955
CciI	TCATGA	1 cut(s)	1176
CfoI	GCGC	4 cut(s)	102, 129, 709, 1800
Cfr10I	RCCGGY	2 cut(s)	653, 2674
Cfr13I	GGNCC	6 cut(s)	644, 669, 1642, 1814, 2110, 2777
CseI	GACGC	2 cut(s)	415, 2339
Csp6I	GTAC	7 cut(s)	75, 336, 366, 1757, 1776, 1893, 2491
CspAI	ACCGGT	2 cut(s)	653, 2674
CviQI	GTAC	7 cut(s)	75, 336, 366, 1757, 1776, 1893, 2491
DdeI	CTNAG	6 cut(s)	45, 1971, 2092, 2557, 3092, 3200
DpnI	GATC	6 cut(s)	317, 808, 871, 1133, 1292, 3106
DpnII	GATC	6 cut(s)	315, 806, 869, 1131, 1290, 3104
DraIII	CACNNNGTG	1 cut(s)	2553
DriI	GACNNNNNGTC	2 cut(s)	40, 403
EaeI	YGGCCR	1 cut(s)	1314
Eam1104I	CTCTTC	1 cut(s)	2619
Eam1105I	GACNNNNNGTC	2 cut(s)	40, 403
EarI	CTCTTC	1 cut(s)	2619
EciI	GGCGGA	1 cut(s)	3136
Ecl136II	GAGCTC	2 cut(s)	2506, 3163
Eco130I	CCWWGG	2 cut(s)	430, 1437
Eco24I	GRGCYC	4 cut(s)	1081, 1445, 2508, 3165
Eco31I	GGTCTC	3 cut(s)	177, 1712, 2095
Eco47I	GGWCC	4 cut(s)	1642, 1814, 2110, 2777
Eco53kI	GAGCTC	2 cut(s)	2506, 3163
Eco88I	CYCGRG	3 cut(s)	38, 1633, 2876
Eco91I	GGTNACC	1 cut(s)	2443
EcoICRI	GAGCTC	2 cut(s)	2506, 3163
EcoNI	CCTNNNNNAGG	1 cut(s)	540
EcoO109I	RGGNCCY	2 cut(s)	1642, 1814
EcoO65I	GGTNACC	1 cut(s)	2443
EcoRI	GAATTC	1 cut(s)	2863
EcoRII	CCWGG	5 cut(s)	475, 1152, 1713, 2112, 2788
EcoT14I	CCWWGG	2 cut(s)	430, 1437
EcoT38I	GRGCYC	4 cut(s)	1081, 1445, 2508, 3165
ErhI	CCWWGG	2 cut(s)	430, 1437
FaqI	GGGAC	4 cut(s)	277, 1432, 2386, 2435
FauI	CCCGC	2 cut(s)	1653, 2950
Fnu4HI	GCNGC	5 cut(s)	342, 502, 606, 2038, 2577
FriOI	GRGCYC	4 cut(s)	1081, 1445, 2508, 3165
Fsp4HI	GCNGC	5 cut(s)	342, 502, 606, 2038, 2577
FspBI	CTAG	5 cut(s)	1985, 2018, 2127, 2618, 3158
FspI	TGCGCA	1 cut(s)	128
GlaI	GCGC	4 cut(s)	101, 128, 708, 1799
GluI	GCNGC	5 cut(s)	342, 502, 606, 2038, 2577
GsuI	CTGGAG	3 cut(s)	321, 344, 1736
HaeII	RGCGCY	1 cut(s)	103
HaeIII	GGCC	7 cut(s)	645, 671, 773, 1206, 1316, 1406, 2295
HapII	CCGG	5 cut(s)	654, 882, 1075, 1203, 2675
HgaI	GACGC	2 cut(s)	415, 2339
HhaI	GCGC	4 cut(s)	102, 129, 709, 1800
Hin6I	GCGC	4 cut(s)	100, 127, 707, 1798
HinP1I	GCGC	4 cut(s)	100, 127, 707, 1798
HincII	GTYRAC	1 cut(s)	2077
HindII	GTYRAC	1 cut(s)	2077
HindIII	AAGCTT	3 cut(s)	1575, 2055, 2733
HpaII	CCGG	5 cut(s)	654, 882, 1075, 1203, 2675
Hpy166II	GTNNAC	3 cut(s)	25, 1504, 2077
Hpy8I	GTNNAC	3 cut(s)	25, 1504, 2077
HpyCH4III	ACNGT	3 cut(s)	55, 1544, 2831
HpyCH4IV	ACGT	2 cut(s)	1013, 2889
HpyF10VI	GCNNNNNNNGC	6 cut(s)	239, 350, 579, 677, 2576, 2936
HpyF3I	CTNAG	6 cut(s)	45, 1971, 2092, 2557, 3092, 3200
HpySE526I	ACGT	2 cut(s)	1013, 2889
HspAI	GCGC	4 cut(s)	100, 127, 707, 1798
Kzo9I	GATC	6 cut(s)	315, 806, 869, 1131, 1290, 3104
LmnI	GCTCC	8 cut(s)	538, 564, 1653, 2249, 2268, 2338, 2641, 3168
Lsp1109I	GCAGC	4 cut(s)	328, 592, 2024, 2588
LweI	GCATC	7 cut(s)	220, 229, 340, 413, 591, 1599, 2802
MaeI	CTAG	5 cut(s)	1985, 2018, 2127, 2618, 3158
MaeII	ACGT	2 cut(s)	1013, 2889
MaeIII	GTNAC	8 cut(s)	385, 845, 1009, 1357, 1432, 1546, 2443, 2608
MalI	GATC	6 cut(s)	317, 808, 871, 1133, 1292, 3106
MbiI	CCGCTC	1 cut(s)	1648
MboI	GATC	6 cut(s)	315, 806, 869, 1131, 1290, 3104
MfeI	CAATTG	3 cut(s)	2571, 2585, 3054
MflI	RGATCY	4 cut(s)	806, 869, 1131, 3104
MhlI	GDGCHC	8 cut(s)	27, 517, 1081, 1445, 1736, 2197, 2508, 3165
MlsI	TGGCCA	1 cut(s)	1316
MluNI	TGGCCA	1 cut(s)	1316
MlyI	GAGTC	6 cut(s)	50, 413, 2082, 2325, 2605, 2822
MmeI	TCCRAC	5 cut(s)	1268, 2293, 2299, 2360, 2672
Mox20I	TGGCCA	1 cut(s)	1316
MroXI	GAANNNNTTC	1 cut(s)	959
MscI	TGGCCA	1 cut(s)	1316
MslI	CAYNNNNRTG	6 cut(s)	779, 1569, 1851, 2366, 2606, 3113
Msp20I	TGGCCA	1 cut(s)	1316
MspCI	CTTAAG	1 cut(s)	1217
MspI	CCGG	5 cut(s)	654, 882, 1075, 1203, 2675
MspR9I	CCNGG	7 cut(s)	477, 882, 1076, 1154, 1715, 2114, 2790
MunI	CAATTG	3 cut(s)	2571, 2585, 3054
Mva1269I	GAATGC	1 cut(s)	1683
MvaI	CCWGG	5 cut(s)	477, 1154, 1715, 2114, 2790
MvnI	CGCG	1 cut(s)	720
MwoI	GCNNNNNNNGC	6 cut(s)	239, 350, 579, 677, 2576, 2936
NciI	CCSGG	2 cut(s)	882, 1076
NcoI	CCATGG	1 cut(s)	1437
NdeII	GATC	6 cut(s)	315, 806, 869, 1131, 1290, 3104
NmuCI	GTSAC	6 cut(s)	385, 845, 1432, 1546, 2443, 2608
NsbI	TGCGCA	1 cut(s)	128
NspI	RCATGY	3 cut(s)	1006, 1568, 1927
NspV	TTCGAA	1 cut(s)	2185
PaeI	GCATGC	2 cut(s)	1006, 1927
PaeR7I	CTCGAG	3 cut(s)	38, 1633, 2876
PagI	TCATGA	1 cut(s)	1176
PciI	ACATGT	1 cut(s)	1564
PctI	GAATGC	1 cut(s)	1683
PdmI	GAANNNNTTC	1 cut(s)	959
PfeI	GAWTC	6 cut(s)	115, 471, 1590, 2690, 3143, 3191
PflFI	GACNNNGTC	1 cut(s)	1544
PfoI	TCCNGGA	1 cut(s)	1713
PinAI	ACCGGT	2 cut(s)	653, 2674
PkrI	GCNGC	5 cut(s)	343, 503, 607, 2039, 2578
PleI	GAGTC	6 cut(s)	49, 412, 2081, 2324, 2605, 2821
PpsI	GAGTC	6 cut(s)	49, 412, 2081, 2324, 2605, 2821
PpuMI	RGGWCCY	2 cut(s)	1642, 1814
PscI	ACATGT	1 cut(s)	1564
PshBI	ATTAAT	1 cut(s)	3174
Psp124BI	GAGCTC	2 cut(s)	2508, 3165
Psp5II	RGGWCCY	2 cut(s)	1642, 1814
Psp6I	CCWGG	5 cut(s)	475, 1152, 1713, 2112, 2788
PspEI	GGTNACC	1 cut(s)	2443
PspGI	CCWGG	5 cut(s)	475, 1152, 1713, 2112, 2788
PspPI	GGNCC	6 cut(s)	644, 669, 1642, 1814, 2110, 2777
PspPPI	RGGWCCY	2 cut(s)	1642, 1814
PspXI	VCTCGAGB	1 cut(s)	38
PstI	CTGCAG	6 cut(s)	567, 610, 769, 2042, 2955, 3103
PsuI	RGATCY	4 cut(s)	806, 869, 1131, 3104
PsyI	GACNNNGTC	1 cut(s)	1544
RsaI	GTAC	7 cut(s)	76, 337, 367, 1758, 1777, 1894, 2492
RsaNI	GTAC	7 cut(s)	75, 336, 366, 1757, 1776, 1893, 2491
RseI	CAYNNNNRTG	6 cut(s)	779, 1569, 1851, 2366, 2606, 3113
SacI	GAGCTC	2 cut(s)	2508, 3165
SatI	GCNGC	5 cut(s)	342, 502, 606, 2038, 2577
Sau3AI	GATC	6 cut(s)	315, 806, 869, 1131, 1290, 3104
Sau96I	GGNCC	6 cut(s)	644, 669, 1642, 1814, 2110, 2777
SbfI	CCTGCAGG	1 cut(s)	2955
ScaI	AGTACT	4 cut(s)	76, 337, 1758, 1777
SchI	GAGTC	6 cut(s)	50, 413, 2082, 2325, 2605, 2822
ScrFI	CCNGG	7 cut(s)	477, 882, 1076, 1154, 1715, 2114, 2790
SdaI	CCTGCAGG	1 cut(s)	2955
SduI	GDGCHC	8 cut(s)	27, 517, 1081, 1445, 1736, 2197, 2508, 3165
SfaNI	GCATC	7 cut(s)	220, 229, 340, 413, 591, 1599, 2802
SfcI	CTRYAG	8 cut(s)	267, 563, 606, 765, 873, 2038, 2951, 3099
Sfr274I	CTCGAG	3 cut(s)	38, 1633, 2876
SfuI	TTCGAA	1 cut(s)	2185
SinI	GGWCC	4 cut(s)	1642, 1814, 2110, 2777
SlaI	CTCGAG	3 cut(s)	38, 1633, 2876
SmiMI	CAYNNNNRTG	6 cut(s)	779, 1569, 1851, 2366, 2606, 3113
SmlI	CTYRAG	7 cut(s)	38, 1217, 1413, 1633, 1940, 2501, 2876
SmoI	CTYRAG	7 cut(s)	38, 1217, 1413, 1633, 1940, 2501, 2876
SphI	GCATGC	2 cut(s)	1006, 1927
Sse8387I	CCTGCAGG	1 cut(s)	2955
SsiI	CCGC	7 cut(s)	242, 502, 1646, 2051, 2541, 2957, 3147
SspI	AATATT	2 cut(s)	373, 1830
SspMI	CTAG	5 cut(s)	1985, 2018, 2127, 2618, 3158
SstI	GAGCTC	2 cut(s)	2508, 3165
StyD4I	CCNGG	7 cut(s)	475, 880, 1074, 1152, 1713, 2112, 2788
StyI	CCWWGG	2 cut(s)	430, 1437
TaaI	ACNGT	3 cut(s)	55, 1544, 2831
TaiI	ACGT	2 cut(s)	1016, 2892
TaqI	TCGA	6 cut(s)	33, 39, 617, 1634, 2185, 2877
TatI	WGTACW	6 cut(s)	74, 335, 1756, 1775, 1892, 2490
TauI	GCSGC	1 cut(s)	504
TfiI	GAWTC	6 cut(s)	115, 471, 1590, 2690, 3143, 3191
TseFI	GTSAC	6 cut(s)	385, 845, 1432, 1546, 2443, 2608
TseI	GCWGC	4 cut(s)	341, 605, 2037, 2576
Tsp45I	GTSAC	6 cut(s)	385, 845, 1432, 1546, 2443, 2608
TspGWI	ACGGA	3 cut(s)	404, 2439, 2666
Tth111I	GACNNNGTC	1 cut(s)	1544
Vha464I	CTTAAG	1 cut(s)	1217
VneI	GTGCAC	1 cut(s)	23
VpaK11BI	GGWCC	4 cut(s)	1642, 1814, 2110, 2777
VspI	ATTAAT	1 cut(s)	3174
XagI	CCTNNNNNAGG	1 cut(s)	540
XapI	RAATTY	3 cut(s)	1252, 2863, 3180
XbaI	TCTAGA	1 cut(s)	3157
XceI	RCATGY	3 cut(s)	1006, 1568, 1927
XcmI	CCANNNNNNNNNTGG	3 cut(s)	93, 654, 781
XhoI	CTCGAG	3 cut(s)	38, 1633, 2876
XmnI	GAANNNNTTC	1 cut(s)	959
XspI	CTAG	5 cut(s)	1985, 2018, 2127, 2618, 3158
ZrmI	AGTACT	4 cut(s)	76, 337, 1758, 1777

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.