RchiOBHm_Chr7g0224291

FHA domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002999
Physical Location & Seq
Forward (+)
46310550 .. 46316085
5536 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20083

Sequence Viewer

Length: 1014 bp
ATGCTGGACCCTTTAGCTCTCAAGCTCGAACTGCTCAAAGGCCCACGTGAGGGAGAAACCCTAGAGTACCGACCCAGATCCAAGGTCCGAATCGGCCGGGTCGTCCGCGGCAACAACCTCGCCATCAGAGACTCCGGCATTTCCACCAACCACCTCATCATCGAATTCGAATCGGGTAAATGGATGATCCGAGACCTCGGCTCCTCCAACGGAACCATCGTAAACGACACCAAGCTCAAGCCGGACACGCCGTTGGAGCTGAACGACGGCGACGAGATAAAGATCGGGGAGTACACGTCGATCTCCGTCAAGATCGACGGCCACGAACGGAGCCGGTTGAGAAGGAATCCGAGGCGCGGCGCGGTTGTGGAGAATCAGGGCCTGAGATGTAGGAAGGAAGAGAATTCTGAATTGGAGAGAGAGGGAGGAGAGGAAATTGAGGTGGTAGAGAAGCCGAAGAGGGGCCGGGGCCGCGGCCGGCCGAGGAAAGCTAGGGTTTTGAAGAGTGAGGTTGCTGTGTCGGAAGTGAGTTCCAGAGATGTGGAAGCTGTGGTTCCGGATTTGGAGACTTGCAGTGTACAGTGTGGGGGGAGGAAGAGGAAGAATGTGCAACAAGTGCCGCTGATGTGTGAGAAAGATAATGTTGTGGTGGCGGAGAAAGATTCGGGAGAAATTGGTCCCGTTGGTGTTAATGGCGATAAAGGGGATGACTGTAATGTTGGAGTGGGTGGCGATAAGGTTGAAAGTGGTAGTAGGAGCTGTTTTGGTGTTAGTGGTGCTAAGAATGTTGATAAAAAGGGGAATGGTTCTGGTGTTAAACAAGGTTTGGGAAAGATTTGGCAGGAGCCAGATTTGGAGAAGATGACTTTGGATGATTGGTTTGATTATGTGCAAGTTTCTTTGCCGAAAGGGGTTAATGATGAGATTGACAAGATTTGTGCCGGAATGAGAGACAAGGCTAAGCTAGTCCAGGAGTATATAGCACAACACAACAAGGAAAAGTGTCACAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FHA

Protein Analysis

337

Amino Acids

37.2

Weight (kDa)

8.02

Isoelectric Point (pI)

28.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yop-YscD_cpl PF16697 10 - 97 2.8e-11 Inner membrane component of T3SS, cytoplasmic domain
FHA PF00498 29 - 96 8.2e-19 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 108, 357, 362, 474
AccIII TCCGGA 1 cut(s) 556
AciI CCGC 8 cut(s) 106, 108, 357, 362, 472, 474, 620, 653
AclWI GGATC 2 cut(s) 72, 181
AcoI YGGCCR 4 cut(s) 94, 319, 475, 479
AcsI RAATTY 2 cut(s) 164, 403
AcvI CACGTG 1 cut(s) 47
AfaI GTAC 3 cut(s) 68, 293, 579
AfiI CCNNNNNNNGG 4 cut(s) 49, 50, 356, 461
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 2 cut(s) 502, 743
AjiI CACGTC 1 cut(s) 297
AjnI CCWGG 1 cut(s) 969
AjuI GAANNNNNNNTTGG 4 cut(s) 395, 427, 851, 883
AluBI AGCT 8 cut(s) 17, 25, 235, 259, 491, 548, 759, 964
AluI AGCT 8 cut(s) 17, 25, 235, 259, 491, 548, 759, 964
Alw26I GTCTC 4 cut(s) 123, 186, 560, 945
AlwI GGATC 2 cut(s) 72, 181
AlwNI CAGNNNCTG 1 cut(s) 382
Aor13HI TCCGGA 1 cut(s) 556
AoxI GGCC 8 cut(s) 40, 94, 319, 379, 463, 469, 475, 479
ApoI RAATTY 2 cut(s) 164, 403
AspLEI GCGC 2 cut(s) 357, 362
AspS9I GGNCC 7 cut(s) 7, 41, 85, 379, 463, 469, 677
AsuC2I CCSGG 2 cut(s) 98, 467
AsuII TTCGAA 1 cut(s) 168
AvaII GGWCC 3 cut(s) 7, 85, 677
BbrPI CACGTG 1 cut(s) 47
BccI CCATC 2 cut(s) 131, 224
BceAI ACGGC 3 cut(s) 235, 283, 334
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BciT130I CCWGG 1 cut(s) 971
BcnI CCSGG 2 cut(s) 98, 467
BcoDI GTCTC 4 cut(s) 123, 186, 560, 945
BfaI CTAG 3 cut(s) 62, 492, 965
BisI GCNGC 5 cut(s) 109, 358, 472, 475, 620
BlpI GCTNAGC 1 cut(s) 960
BlsI GCNGC 5 cut(s) 110, 359, 473, 476, 621
Bme1390I CCNGG 3 cut(s) 98, 467, 971
Bme18I GGWCC 3 cut(s) 7, 85, 677
BmgBI CACGTC 1 cut(s) 297
BmgT120I GGNCC 7 cut(s) 7, 41, 85, 379, 463, 469, 677
BmiI GGNNCC 9 cut(s) 9, 202, 214, 332, 464, 470, 555, 679, 846
BmrFI CCNGG 3 cut(s) 98, 467, 971
Bpu1102I GCTNAGC 1 cut(s) 960
Bpu14I TTCGAA 1 cut(s) 168
BpuEI CTTGAG 2 cut(s) 5, 221
BpuMI CCSGG 2 cut(s) 98, 467
BsaAI YACGTR 1 cut(s) 47
BsaBI GATNNNNATC 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 186
BsaJI CCNNGG 7 cut(s) 81, 106, 196, 350, 466, 472, 482
BsaWI WCCGGW 1 cut(s) 556
BsaXI ACNNNNNCTCC 4 cut(s) 185, 215, 281, 311
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 50, 356, 461
Bse118I RCCGGY 2 cut(s) 333, 477
Bse8I GATNNNNATC 1 cut(s) 281
BseAI TCCGGA 1 cut(s) 556
BseBI CCWGG 1 cut(s) 971
BseDI CCNNGG 7 cut(s) 81, 106, 196, 350, 466, 472, 482
BseGI GGATG 3 cut(s) 189, 712, 877
BseJI GATNNNNATC 1 cut(s) 281
BseLI CCNNNNNNNGG 4 cut(s) 49, 50, 356, 461
BseMII CTCAG 1 cut(s) 374
BseRI GAGGAG 2 cut(s) 193, 441
BseX3I CGGCCG 3 cut(s) 94, 475, 479
Bsh1236I CGCG 4 cut(s) 108, 357, 362, 474
Bsh1285I CGRYCG 3 cut(s) 97, 478, 482
BshFI GGCC 8 cut(s) 42, 96, 321, 381, 465, 471, 477, 481
BsiEI CGRYCG 3 cut(s) 97, 478, 482
BsiSI CCGG 8 cut(s) 97, 135, 242, 334, 466, 478, 557, 942
BslFI GGGAC 1 cut(s) 663
BslI CCNNNNNNNGG 4 cut(s) 49, 50, 356, 461
BsmAI GTCTC 4 cut(s) 123, 186, 560, 945
BsmFI GGGAC 1 cut(s) 663
BsnI GGCC 8 cut(s) 42, 96, 321, 381, 465, 471, 477, 481
Bso31I GGTCTC 1 cut(s) 186
Bsp119I TTCGAA 1 cut(s) 168
Bsp13I TCCGGA 1 cut(s) 556
Bsp1407I TGTACA 1 cut(s) 577
Bsp143I GATC 5 cut(s) 77, 186, 282, 300, 312
Bsp1720I GCTNAGC 1 cut(s) 960
BspACI CCGC 8 cut(s) 106, 108, 357, 362, 472, 474, 620, 653
BspANI GGCC 8 cut(s) 42, 96, 321, 381, 465, 471, 477, 481
BspCNI CTCAG 1 cut(s) 375
BspEI TCCGGA 1 cut(s) 556
BspFNI CGCG 4 cut(s) 108, 357, 362, 474
BspLI GGNNCC 9 cut(s) 9, 202, 214, 332, 464, 470, 555, 679, 846
BspPI GGATC 2 cut(s) 72, 181
BspT104I TTCGAA 1 cut(s) 168
BspTNI GGTCTC 1 cut(s) 186
BsrFI RCCGGY 2 cut(s) 333, 477
BsrGI TGTACA 1 cut(s) 577
BssAI RCCGGY 2 cut(s) 333, 477
BssECI CCNNGG 7 cut(s) 81, 106, 196, 350, 466, 472, 482
BssMI GATC 5 cut(s) 77, 186, 282, 300, 312
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 971
Bst4CI ACNGT 3 cut(s) 582, 713, 1010
Bst6I CTCTTC 4 cut(s) 393, 452, 497, 590
BstAPI GCANNNNNTGC 1 cut(s) 616
BstAUI TGTACA 1 cut(s) 577
BstBAI YACGTR 1 cut(s) 47
BstBI TTCGAA 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 479
BstDEI CTNAG 3 cut(s) 383, 780, 960
BstDSI CCRYGG 2 cut(s) 106, 472
BstF5I GGATG 3 cut(s) 189, 712, 877
BstFNI CGCG 4 cut(s) 108, 357, 362, 474
BstHHI GCGC 2 cut(s) 357, 362
BstKTI GATC 5 cut(s) 80, 189, 285, 303, 315
BstMAI GTCTC 4 cut(s) 123, 186, 560, 945
BstMBI GATC 5 cut(s) 77, 186, 282, 300, 312
BstMCI CGRYCG 3 cut(s) 97, 478, 482
BstMWI GCNNNNNNNGC 5 cut(s) 31, 247, 256, 471, 616
BstNI CCWGG 1 cut(s) 971
BstSCI CCNGG 3 cut(s) 96, 465, 969
BstUI CGCG 4 cut(s) 108, 357, 362, 474
BstX2I RGATCY 1 cut(s) 77
BstXI CCANNNNNNTGG 1 cut(s) 541
BstYI RGATCY 1 cut(s) 77
BstZI CGGCCG 3 cut(s) 94, 475, 479
BsuRI GGCC 8 cut(s) 42, 96, 321, 381, 465, 471, 477, 481
BtgI CCRYGG 2 cut(s) 106, 472
BtrI CACGTC 1 cut(s) 297
BtsCI GGATG 3 cut(s) 189, 712, 877
BtsI GCAGTG 1 cut(s) 580
BtsIMutI CAGTG 2 cut(s) 580, 587
Cac8I GCNNGC 1 cut(s) 479
CaiI CAGNNNCTG 1 cut(s) 382
CfoI GCGC 2 cut(s) 357, 362
Cfr10I RCCGGY 2 cut(s) 333, 477
Cfr13I GGNCC 7 cut(s) 7, 41, 85, 379, 463, 469, 677
Cfr42I CCGCGG 2 cut(s) 109, 475
Csp6I GTAC 3 cut(s) 67, 292, 578
CviQI GTAC 3 cut(s) 67, 292, 578
DdeI CTNAG 3 cut(s) 383, 780, 960
DpnI GATC 5 cut(s) 79, 188, 284, 302, 314
DpnII GATC 5 cut(s) 77, 186, 282, 300, 312
EaeI YGGCCR 4 cut(s) 94, 319, 475, 479
EagI CGGCCG 3 cut(s) 94, 475, 479
Eam1104I CTCTTC 4 cut(s) 393, 452, 497, 590
EarI CTCTTC 4 cut(s) 393, 452, 497, 590
EciI GGCGGA 1 cut(s) 668
EclXI CGGCCG 3 cut(s) 94, 475, 479
Eco130I CCWWGG 1 cut(s) 81
Eco31I GGTCTC 1 cut(s) 186
Eco47I GGWCC 3 cut(s) 7, 85, 677
Eco52I CGGCCG 3 cut(s) 94, 475, 479
Eco72I CACGTG 1 cut(s) 47
EcoO109I RGGNCCY 1 cut(s) 379
EcoRI GAATTC 2 cut(s) 164, 403
EcoRII CCWGG 1 cut(s) 969
EcoT14I CCWWGG 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 81
FaiI YATR 3 cut(s) 888, 978, 980
FaqI GGGAC 1 cut(s) 663
Fnu4HI GCNGC 5 cut(s) 109, 358, 472, 475, 620
FokI GGATG 3 cut(s) 196, 719, 884
FseI GGCCGGCC 1 cut(s) 481
Fsp4HI GCNGC 5 cut(s) 109, 358, 472, 475, 620
FspBI CTAG 3 cut(s) 62, 492, 965
GlaI GCGC 2 cut(s) 356, 361
GluI GCNGC 5 cut(s) 109, 358, 472, 475, 620
HaeIII GGCC 8 cut(s) 42, 96, 321, 381, 465, 471, 477, 481
HapII CCGG 8 cut(s) 97, 135, 242, 334, 466, 478, 557, 942
HhaI GCGC 2 cut(s) 357, 362
Hin6I GCGC 2 cut(s) 355, 360
HinP1I GCGC 2 cut(s) 355, 360
HinfI GANTC 6 cut(s) 90, 131, 170, 346, 373, 662
HpaII CCGG 8 cut(s) 97, 135, 242, 334, 466, 478, 557, 942
Hpy166II GTNNAC 3 cut(s) 223, 294, 578
Hpy188I TCNGA 6 cut(s) 89, 128, 191, 351, 409, 523
Hpy188III TCNNGA 4 cut(s) 310, 534, 557, 666
Hpy8I GTNNAC 3 cut(s) 223, 294, 578
Hpy99I CGWCG 4 cut(s) 269, 275, 301, 320
HpyAV CCTTC 2 cut(s) 336, 388
HpyCH4III ACNGT 3 cut(s) 582, 713, 1010
HpyCH4IV ACGT 2 cut(s) 46, 296
HpyCH4V TGCA 3 cut(s) 573, 610, 892
HpyF10VI GCNNNNNNNGC 5 cut(s) 31, 247, 256, 471, 616
HpyF3I CTNAG 3 cut(s) 383, 780, 960
HpySE526I ACGT 2 cut(s) 46, 296
HspAI GCGC 2 cut(s) 355, 360
Kpn2I TCCGGA 1 cut(s) 556
KroI GCCGGC 1 cut(s) 477
KroNI GCCGGC 1 cut(s) 479
KspI CCGCGG 2 cut(s) 109, 475
Kzo9I GATC 5 cut(s) 77, 186, 282, 300, 312
LmnI GCTCC 5 cut(s) 206, 256, 330, 756, 844
MaeI CTAG 3 cut(s) 62, 492, 965
MaeII ACGT 2 cut(s) 46, 296
MaeIII GTNAC 1 cut(s) 1004
MalI GATC 5 cut(s) 79, 188, 284, 302, 314
MboI GATC 5 cut(s) 77, 186, 282, 300, 312
MboII GAAGA 6 cut(s) 410, 469, 514, 607, 613, 871
MflI RGATCY 1 cut(s) 77
MluCI AATT 5 cut(s) 164, 403, 410, 435, 672
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 4 cut(s) 231, 234, 501, 700
MroI TCCGGA 1 cut(s) 556
MroNI GCCGGC 1 cut(s) 477
MseI TTAA 4 cut(s) 690, 816, 915, 1012
MspA1I CMGCKG 3 cut(s) 108, 474, 622
MspI CCGG 8 cut(s) 97, 135, 242, 334, 466, 478, 557, 942
MspR9I CCNGG 3 cut(s) 98, 467, 971
MteI GCGCNGCGC 1 cut(s) 358
MvaI CCWGG 1 cut(s) 971
MvnI CGCG 4 cut(s) 108, 357, 362, 474
MwoI GCNNNNNNNGC 5 cut(s) 31, 247, 256, 471, 616
NaeI GCCGGC 1 cut(s) 479
NciI CCSGG 2 cut(s) 98, 467
NdeII GATC 5 cut(s) 77, 186, 282, 300, 312
NgoMIV GCCGGC 1 cut(s) 477
NlaIV GGNNCC 9 cut(s) 9, 202, 214, 332, 464, 470, 555, 679, 846
NmeAIII GCCGAG 2 cut(s) 177, 507
NmuCI GTSAC 1 cut(s) 1004
NspV TTCGAA 1 cut(s) 168
PcsI WCGNNNNNNNCGW 4 cut(s) 99, 216, 270, 321
PdiI GCCGGC 1 cut(s) 479
PfeI GAWTC 5 cut(s) 90, 170, 346, 373, 662
PfoI TCCNGGA 1 cut(s) 969
PkrI GCNGC 5 cut(s) 110, 359, 473, 476, 621
PleI GAGTC 1 cut(s) 125
PmaCI CACGTG 1 cut(s) 47
PmlI CACGTG 1 cut(s) 47
PpsI GAGTC 1 cut(s) 125
Ppu21I YACGTR 1 cut(s) 47
Psp6I CCWGG 1 cut(s) 969
PspCI CACGTG 1 cut(s) 47
PspGI CCWGG 1 cut(s) 969
PspN4I GGNNCC 9 cut(s) 9, 202, 214, 332, 464, 470, 555, 679, 846
PspPI GGNCC 7 cut(s) 7, 41, 85, 379, 463, 469, 677
PstNI CAGNNNCTG 1 cut(s) 382
PsuI RGATCY 1 cut(s) 77
RigI GGCCGGCC 1 cut(s) 481
RsaI GTAC 3 cut(s) 68, 293, 579
RsaNI GTAC 3 cut(s) 67, 292, 578
SacII CCGCGG 2 cut(s) 109, 475
SaqAI TTAA 4 cut(s) 690, 816, 915, 1012
SatI GCNGC 5 cut(s) 109, 358, 472, 475, 620
Sau3AI GATC 5 cut(s) 77, 186, 282, 300, 312
Sau96I GGNCC 7 cut(s) 7, 41, 85, 379, 463, 469, 677
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 3 cut(s) 98, 467, 971
Sfr303I CCGCGG 2 cut(s) 109, 475
SfuI TTCGAA 1 cut(s) 168
SgrBI CCGCGG 2 cut(s) 109, 475
SinI GGWCC 3 cut(s) 7, 85, 677
SmlI CTYRAG 2 cut(s) 20, 236
SmoI CTYRAG 2 cut(s) 20, 236
Sse9I AATT 5 cut(s) 164, 403, 410, 435, 672
SsiI CCGC 8 cut(s) 106, 108, 357, 362, 472, 474, 620, 653
SspMI CTAG 3 cut(s) 62, 492, 965
StyD4I CCNGG 3 cut(s) 96, 465, 969
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 3 cut(s) 582, 713, 1010
TaiI ACGT 2 cut(s) 49, 299
TaqI TCGA 5 cut(s) 27, 162, 168, 299, 315
TasI AATT 5 cut(s) 164, 403, 410, 435, 672
TatI WGTACW 2 cut(s) 291, 577
TauI GCSGC 5 cut(s) 111, 360, 474, 477, 622
TfiI GAWTC 5 cut(s) 90, 170, 346, 373, 662
Tru1I TTAA 4 cut(s) 690, 816, 915, 1012
Tru9I TTAA 4 cut(s) 690, 816, 915, 1012
TscAI CASTG 2 cut(s) 580, 587
TseFI GTSAC 1 cut(s) 1004
Tsp45I GTSAC 1 cut(s) 1004
TspGWI ACGGA 3 cut(s) 225, 295, 343
TspRI CASTG 2 cut(s) 580, 587
VpaK11BI GGWCC 3 cut(s) 7, 85, 677
XapI RAATTY 2 cut(s) 164, 403
XspI CTAG 3 cut(s) 62, 492, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.