Rroxscaffold_5G00336920

FHA domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002999
Physical Location & Seq
Forward (+)
4810303 .. 4814988
4686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00336920.1

Sequence Viewer

Length: 1218 bp
ATGATCCGAGACCTCGACTCCTCCAACGGAACCATCGTAAACGGCACCAAGCTCAAGCCGGACACGCCGTTGGAGCTGAGCGACGGCGACGAGATCAAGATTGGGGAGTACACGTCGATCTCCGTCAAGATCGACGGCCACGAAGGGAGCAGGTTGAGAAGGAATCCGAGGCGTGGCGCGGCGGGGAAGGTGGGGACGGCTGCGGAAAGTCGGGGCCGGAGAGGGAGGGTTAAGGAGAGTGAGGTCGAAGTGAAATTGGAGGTGGAGATTGAGAATGATGAGGAAATTAGTGTAGCAGCACCGGTCGCTGAGAATCAGGGCCGGAGAGGTAGGGCTGCGAAGAAGAAGGAAGAGAATTCTGAATTGGATAGAAAGGGAGAGGAAATTGAGGTGGTGGAGAAACCGAAGAGGGGCCGGGGCCGGCCGAGGAAAGCTAGGGTTTTGAAGAGTGAGGATTTGGTGGAGGAGGTTGCTGTGCCGGAAGTGAGTTCGAGAGCAACTCAAAGGTGTAAGGATGTGGAAGCTGTGGTTCTGGATTCGGAGAATTGCAGTGTAGAGTTTGAACAAGTGAAGATTGAACCTAAGAGGCGTGGTGGGGGTAGGAAGAGGAAGAATGTGCAAGAAGTGCCGCGGCTGTGTGAGGAAGGTAATGTTGCGGCGGAGAAAGATTTGGGAGAAATTGGTTCAGTTGGTGTTAATGGCGATAATGAAGATAAAGGGGATGTTCCAGAGCAGAAGGGTTCGGGAGGAATTGGTCCAGTTGATATCAAGGAGAATGGCGTGTATGATGATGATGCTAAAGGGTTGAATTTTGGGGATGAAGAGGCAGCAGTAGGTTTTGATGTGGGAGAGAGTGGCAATGATAATGAGAAGGCAAAAGGGTCCAATTTGGGCCATGAAGAGGCAGCCATTGACTGTAATGTAGGAGAGAGTGGCGATAAGGTTGGAAGTGGTAGTAGGAGCTGTTTTAGTGTTAGTGGTGCTAATGATGTTGATAAAAAGGGGACTGACTCTGGTGTTAAAGAAGGTTTGAGAAAGGTTTGGCAGGAGCCAGATTTGGAGAAGATGACTTTGGACGATTGGTTTGATTATGTGGAATTTTCTTTGCCGAAAGCGGTTAATGATGAGATTGACAAGATTTGTTCCGGAATGAGAGAGAAGGCTAAGCTAGTCCAGGAGTATATAGCAAAACACAAGAAGGAGAAGTGTCAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FHA

Protein Analysis

405

Amino Acids

44.11

Weight (kDa)

5.06

Isoelectric Point (pI)

38.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FHA PF00498 2 - 35 1.7e-08 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 141
AccB1I GGYRCC 1 cut(s) 44
AccII CGCG 2 cut(s) 179, 631
AccIII TCCGGA 1 cut(s) 1145
AciI CCGC 8 cut(s) 179, 182, 203, 629, 631, 656, 659, 1115
AcoI YGGCCR 2 cut(s) 136, 422
AcsI RAATTY 3 cut(s) 355, 808, 1097
AfaI GTAC 1 cut(s) 110
AfiI CCNNNNNNNGG 3 cut(s) 173, 410, 901
AflIII ACRYGT 1 cut(s) 111
AgeI ACCGGT 1 cut(s) 301
AgsI TTSAA 4 cut(s) 445, 563, 578, 808
AjiI CACGTC 1 cut(s) 114
AjnI CCWGG 1 cut(s) 1173
AjuI GAANNNNNNNTTGG 4 cut(s) 347, 379, 1055, 1087
AluBI AGCT 6 cut(s) 52, 76, 434, 524, 963, 1168
AluI AGCT 6 cut(s) 52, 76, 434, 524, 963, 1168
Alw26I GTCTC 1 cut(s) 3
Aor13HI TCCGGA 1 cut(s) 1145
AoxI GGCC 7 cut(s) 136, 214, 319, 412, 418, 422, 892
ApeKI GCWGC 5 cut(s) 200, 296, 335, 827, 905
ApoI RAATTY 3 cut(s) 355, 808, 1097
ArsI GACNNNNNNTTYG 2 cut(s) 1067, 1099
AsiGI ACCGGT 1 cut(s) 301
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 7 cut(s) 214, 319, 412, 418, 755, 882, 892
AsuC2I CCSGG 1 cut(s) 416
AvaII GGWCC 2 cut(s) 755, 882
BanI GGYRCC 1 cut(s) 44
BbvI GCAGC 5 cut(s) 187, 308, 322, 839, 917
BccI CCATC 1 cut(s) 41
BceAI ACGGC 5 cut(s) 52, 58, 100, 151, 213
BciT130I CCWGG 1 cut(s) 1175
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 1 cut(s) 3
BfaI CTAG 2 cut(s) 435, 1169
BfuAI ACCTGC 1 cut(s) 141
BisI GCNGC 9 cut(s) 180, 201, 297, 336, 629, 632, 657, 828, 906
BlpI GCTNAGC 2 cut(s) 77, 1164
BlsI GCNGC 9 cut(s) 181, 202, 298, 337, 630, 633, 658, 829, 907
Bme1390I CCNGG 2 cut(s) 416, 1175
Bme18I GGWCC 2 cut(s) 755, 882
BmgBI CACGTC 1 cut(s) 114
BmgT120I GGNCC 7 cut(s) 214, 319, 412, 418, 755, 882, 892
BmiI GGNNCC 7 cut(s) 31, 46, 215, 413, 419, 883, 1050
BmrFI CCNGG 2 cut(s) 416, 1175
BmsI GCATC 1 cut(s) 784
BplI GAGNNNNNCTC 2 cut(s) 484, 516
Bpu1102I GCTNAGC 2 cut(s) 77, 1164
BpuEI CTTGAG 1 cut(s) 38
BpuMI CCSGG 1 cut(s) 416
BsaI GGTCTC 1 cut(s) 3
BsaJI CCNNGG 4 cut(s) 167, 415, 425, 629
BsaWI WCCGGW 2 cut(s) 301, 1145
BsaXI ACNNNNNCTCC 3 cut(s) 32, 98, 128
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 410, 901
Bse118I RCCGGY 2 cut(s) 301, 420
Bse1I ACTGG 1 cut(s) 758
Bse3DI GCAATG 1 cut(s) 865
BseAI TCCGGA 1 cut(s) 1145
BseBI CCWGG 1 cut(s) 1175
BseDI CCNNGG 4 cut(s) 167, 415, 425, 629
BseGI GGATG 3 cut(s) 520, 727, 823
BseLI CCNNNNNNNGG 3 cut(s) 173, 410, 901
BseMI GCAATG 1 cut(s) 865
BseMII CTCAG 2 cut(s) 68, 300
BseNI ACTGG 1 cut(s) 758
BseRI GAGGAG 2 cut(s) 10, 479
BseX3I CGGCCG 1 cut(s) 422
BseXI GCAGC 5 cut(s) 187, 308, 322, 839, 917
Bsh1236I CGCG 2 cut(s) 179, 631
Bsh1285I CGRYCG 2 cut(s) 306, 425
BshFI GGCC 7 cut(s) 138, 216, 321, 414, 420, 424, 894
BshNI GGYRCC 1 cut(s) 44
BshTI ACCGGT 1 cut(s) 301
BsiEI CGRYCG 2 cut(s) 306, 425
BsiSI CCGG 8 cut(s) 59, 217, 302, 322, 415, 421, 479, 1146
BslFI GGGAC 2 cut(s) 208, 1018
BslI CCNNNNNNNGG 3 cut(s) 173, 410, 901
BsmAI GTCTC 1 cut(s) 3
BsmFI GGGAC 2 cut(s) 208, 1018
BsnI GGCC 7 cut(s) 138, 216, 321, 414, 420, 424, 894
Bso31I GGTCTC 1 cut(s) 3
Bsp13I TCCGGA 1 cut(s) 1145
Bsp143I GATC 4 cut(s) 3, 93, 117, 129
Bsp1720I GCTNAGC 2 cut(s) 77, 1164
BspACI CCGC 8 cut(s) 179, 182, 203, 629, 631, 656, 659, 1115
BspANI GGCC 7 cut(s) 138, 216, 321, 414, 420, 424, 894
BspCNI CTCAG 2 cut(s) 69, 301
BspEI TCCGGA 1 cut(s) 1145
BspFNI CGCG 2 cut(s) 179, 631
BspLI GGNNCC 7 cut(s) 31, 46, 215, 413, 419, 883, 1050
BspMI ACCTGC 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 44
BspTNI GGTCTC 1 cut(s) 3
BsrDI GCAATG 1 cut(s) 865
BsrFI RCCGGY 2 cut(s) 301, 420
BsrI ACTGG 1 cut(s) 758
BssAI RCCGGY 2 cut(s) 301, 420
BssECI CCNNGG 4 cut(s) 167, 415, 425, 629
BssMI GATC 4 cut(s) 3, 93, 117, 129
Bst2UI CCWGG 1 cut(s) 1175
Bst4CI ACNGT 1 cut(s) 917
Bst6I CTCTTC 6 cut(s) 345, 401, 440, 599, 816, 894
BstAPI GCANNNNNTGC 1 cut(s) 625
BstC8I GCNNGC 1 cut(s) 422
BstDEI CTNAG 4 cut(s) 77, 309, 582, 1164
BstDSI CCRYGG 1 cut(s) 629
BstF5I GGATG 3 cut(s) 520, 727, 823
BstFNI CGCG 2 cut(s) 179, 631
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 4 cut(s) 6, 96, 120, 132
BstMAI GTCTC 1 cut(s) 3
BstMBI GATC 4 cut(s) 3, 93, 117, 129
BstMCI CGRYCG 2 cut(s) 306, 425
BstMWI GCNNNNNNNGC 4 cut(s) 64, 73, 305, 625
BstNI CCWGG 1 cut(s) 1175
BstSCI CCNGG 2 cut(s) 414, 1173
BstUI CGCG 2 cut(s) 179, 631
BstV1I GCAGC 5 cut(s) 187, 308, 322, 839, 917
BstZI CGGCCG 1 cut(s) 422
BsuRI GGCC 7 cut(s) 138, 216, 321, 414, 420, 424, 894
BtgI CCRYGG 1 cut(s) 629
BtrI CACGTC 1 cut(s) 114
BtsCI GGATG 3 cut(s) 520, 727, 823
BtsI GCAGTG 1 cut(s) 556
BtsIMutI CAGTG 1 cut(s) 556
BveI ACCTGC 1 cut(s) 141
Cac8I GCNNGC 1 cut(s) 422
CfoI GCGC 1 cut(s) 179
Cfr10I RCCGGY 2 cut(s) 301, 420
Cfr13I GGNCC 7 cut(s) 214, 319, 412, 418, 755, 882, 892
Cfr42I CCGCGG 1 cut(s) 632
Csp6I GTAC 1 cut(s) 109
CspAI ACCGGT 1 cut(s) 301
CviAII CATG 1 cut(s) 896
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 4 cut(s) 77, 309, 582, 1164
DpnI GATC 4 cut(s) 5, 95, 119, 131
DpnII GATC 4 cut(s) 3, 93, 117, 129
EaeI YGGCCR 2 cut(s) 136, 422
EagI CGGCCG 1 cut(s) 422
Eam1104I CTCTTC 6 cut(s) 345, 401, 440, 599, 816, 894
EarI CTCTTC 6 cut(s) 345, 401, 440, 599, 816, 894
EciI GGCGGA 1 cut(s) 674
EclXI CGGCCG 1 cut(s) 422
Eco31I GGTCTC 1 cut(s) 3
Eco32I GATATC 1 cut(s) 766
Eco47I GGWCC 2 cut(s) 755, 882
Eco52I CGGCCG 1 cut(s) 422
EcoRI GAATTC 1 cut(s) 355
EcoRII CCWGG 1 cut(s) 1173
EcoRV GATATC 1 cut(s) 766
FaeI CATG 1 cut(s) 899
FaiI YATR 5 cut(s) 786, 897, 1092, 1182, 1184
FaqI GGGAC 2 cut(s) 208, 1018
FatI CATG 1 cut(s) 895
FauI CCCGC 1 cut(s) 175
Fnu4HI GCNGC 9 cut(s) 180, 201, 297, 336, 629, 632, 657, 828, 906
FokI GGATG 3 cut(s) 527, 734, 830
FseI GGCCGGCC 1 cut(s) 424
Fsp4HI GCNGC 9 cut(s) 180, 201, 297, 336, 629, 632, 657, 828, 906
FspBI CTAG 2 cut(s) 435, 1169
GlaI GCGC 1 cut(s) 178
GluI GCNGC 9 cut(s) 180, 201, 297, 336, 629, 632, 657, 828, 906
HaeIII GGCC 7 cut(s) 138, 216, 321, 414, 420, 424, 894
HapII CCGG 8 cut(s) 59, 217, 302, 322, 415, 421, 479, 1146
HhaI GCGC 1 cut(s) 179
Hin1II CATG 1 cut(s) 899
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HinfI GANTC 5 cut(s) 17, 163, 313, 536, 1010
HpaII CCGG 8 cut(s) 59, 217, 302, 322, 415, 421, 479, 1146
Hpy166II GTNNAC 2 cut(s) 40, 111
Hpy188I TCNGA 4 cut(s) 8, 168, 361, 541
Hpy188III TCNNGA 7 cut(s) 97, 127, 492, 533, 728, 744, 1146
Hpy8I GTNNAC 2 cut(s) 40, 111
Hpy99I CGWCG 4 cut(s) 86, 92, 118, 137
HpyCH4III ACNGT 1 cut(s) 917
HpyCH4IV ACGT 1 cut(s) 113
HpyCH4V TGCA 2 cut(s) 549, 619
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 73, 305, 625
HpyF3I CTNAG 4 cut(s) 77, 309, 582, 1164
HpySE526I ACGT 1 cut(s) 113
Hsp92II CATG 1 cut(s) 899
HspAI GCGC 1 cut(s) 177
Kpn2I TCCGGA 1 cut(s) 1145
KroI GCCGGC 1 cut(s) 420
KroNI GCCGGC 1 cut(s) 422
KspI CCGCGG 1 cut(s) 632
Kzo9I GATC 4 cut(s) 3, 93, 117, 129
LmnI GCTCC 4 cut(s) 73, 147, 960, 1048
Lsp1109I GCAGC 5 cut(s) 187, 308, 322, 839, 917
LweI GCATC 1 cut(s) 784
MaeI CTAG 2 cut(s) 435, 1169
MaeII ACGT 1 cut(s) 113
MalI GATC 4 cut(s) 5, 95, 119, 131
MboI GATC 4 cut(s) 3, 93, 117, 129
MlyI GAGTC 2 cut(s) 11, 1004
MmeI TCCRAC 3 cut(s) 48, 51, 925
MroI TCCGGA 1 cut(s) 1145
MroNI GCCGGC 1 cut(s) 420
MseI TTAA 5 cut(s) 231, 696, 1020, 1119, 1216
MspA1I CMGCKG 1 cut(s) 631
MspI CCGG 8 cut(s) 59, 217, 302, 322, 415, 421, 479, 1146
MspR9I CCNGG 2 cut(s) 416, 1175
MvaI CCWGG 1 cut(s) 1175
MvnI CGCG 2 cut(s) 179, 631
MwoI GCNNNNNNNGC 4 cut(s) 64, 73, 305, 625
NaeI GCCGGC 1 cut(s) 422
NciI CCSGG 1 cut(s) 416
NdeII GATC 4 cut(s) 3, 93, 117, 129
NgoMIV GCCGGC 1 cut(s) 420
NlaIII CATG 1 cut(s) 899
NlaIV GGNNCC 7 cut(s) 31, 46, 215, 413, 419, 883, 1050
NmeAIII GCCGAG 1 cut(s) 450
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 138
PdiI GCCGGC 1 cut(s) 422
PfeI GAWTC 3 cut(s) 163, 313, 536
PfoI TCCNGGA 1 cut(s) 1173
PinAI ACCGGT 1 cut(s) 301
PkrI GCNGC 9 cut(s) 181, 202, 298, 337, 630, 633, 658, 829, 907
PleI GAGTC 2 cut(s) 11, 1004
PpsI GAGTC 2 cut(s) 11, 1004
Psp6I CCWGG 1 cut(s) 1173
PspGI CCWGG 1 cut(s) 1173
PspN4I GGNNCC 7 cut(s) 31, 46, 215, 413, 419, 883, 1050
PspPI GGNCC 7 cut(s) 214, 319, 412, 418, 755, 882, 892
RigI GGCCGGCC 1 cut(s) 424
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
SacII CCGCGG 1 cut(s) 632
SaqAI TTAA 5 cut(s) 231, 696, 1020, 1119, 1216
SatI GCNGC 9 cut(s) 180, 201, 297, 336, 629, 632, 657, 828, 906
Sau3AI GATC 4 cut(s) 3, 93, 117, 129
Sau96I GGNCC 7 cut(s) 214, 319, 412, 418, 755, 882, 892
SchI GAGTC 2 cut(s) 11, 1004
ScrFI CCNGG 2 cut(s) 416, 1175
SfaNI GCATC 1 cut(s) 784
Sfr303I CCGCGG 1 cut(s) 632
SgrBI CCGCGG 1 cut(s) 632
SinI GGWCC 2 cut(s) 755, 882
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
SsiI CCGC 8 cut(s) 179, 182, 203, 629, 631, 656, 659, 1115
SspMI CTAG 2 cut(s) 435, 1169
StyD4I CCNGG 2 cut(s) 414, 1173
TaaI ACNGT 1 cut(s) 917
TaiI ACGT 1 cut(s) 116
TaqI TCGA 5 cut(s) 15, 116, 132, 246, 491
TatI WGTACW 1 cut(s) 108
TauI GCSGC 4 cut(s) 182, 631, 634, 659
TfiI GAWTC 3 cut(s) 163, 313, 536
Tru1I TTAA 5 cut(s) 231, 696, 1020, 1119, 1216
Tru9I TTAA 5 cut(s) 231, 696, 1020, 1119, 1216
TscAI CASTG 1 cut(s) 556
TseI GCWGC 5 cut(s) 200, 296, 335, 827, 905
TspDTI ATGAA 3 cut(s) 723, 834, 912
TspGWI ACGGA 2 cut(s) 42, 112
TspRI CASTG 1 cut(s) 556
VpaK11BI GGWCC 2 cut(s) 755, 882
XapI RAATTY 3 cut(s) 355, 808, 1097
XspI CTAG 2 cut(s) 435, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.