RLG00000015515

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003749
Physical Location & Seq
Reverse (-)
67313809 .. 67315464
1656 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015515

Sequence Viewer

Length: 852 bp
ATGGACTTCGGTGGCCTTGATCCGGATGATGTTATCATCGAAAGGGCACTTCATGGTACTCGTCTATTCGAAGCGGTAAGATTACTTCCAGATTATGGGGATGCAATACCAAAGAGATCAGCAGGGGGCACATTTCAGGTTGAATCAAAAATAGTCTCTCGAGCTCCTAGTCAATCAAGATATCGGTTACATACTGATGATGAATCTAATATCTCATCTTCACCAATACAACATCATGACCCATCTGATAATGTTGGCTCTACCCCAAGAAACTCACCAGTTGGTCATGACCACCCACTTCCACAAAGAGAAACTCGTGAACATGACCTCACAGGCGTTCAAAATCTTGAGACTTTAGATCTTGAAGGATGTAAGACTTTGGTGAAACTTTATGAATCCCTCGGACAACTCAAAAGACTTATTGTATTGAATCTTAAAGATTGCAAGAGTCTCGAGAGTCTGCCAAGTAAGATTGAAATGGAATCTCTTGAAACTTTAATTCTATCTAAGTGTTCAAAAGTTAAAAAGATTCCTGAGTTCGCTGGAAATATGATGCGTTTATCAAAGCTTTATCTCGATGGGACTGCCATTGAAAAACTACATGTGTCAATTGAATGCCTGAGTGGCCTTGCTTCGCTGAATCTGAGCAACTGCAAAAATCTTGTTTGCCTTCCAAGCACCTTTAGCAGATTGGAGTCTCTTGAAAACCTTAATCTTTCTGGATGTTTGAAACTTGGCGAACAGGAAAGTGTGAGGGAGAATGGTGCAATTAGAACTGCTGGGAAATATTTGTCATGTTTTACTGTGAGAAAGATTGTTGCGAGAAAGATAATTGTGAAGTTGTTGGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

284

Amino Acids

31.29

Weight (kDa)

7.6

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_13 PF23286 161 - 247 1.7e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 95
AccIII TCCGGA 1 cut(s) 22
AciI CCGC 1 cut(s) 74
AclWI GGATC 1 cut(s) 14
AfaI GTAC 1 cut(s) 58
AfiI CCNNNNNNNGG 2 cut(s) 22, 95
AflIII ACRYGT 1 cut(s) 601
AluBI AGCT 2 cut(s) 164, 568
AluI AGCT 2 cut(s) 164, 568
Alw21I GWGCWC 1 cut(s) 166
Alw26I GTCTC 4 cut(s) 160, 344, 455, 702
AlwI GGATC 1 cut(s) 14
Ama87I CYCGRG 2 cut(s) 159, 452
Aor13HI TCCGGA 1 cut(s) 22
AoxI GGCC 2 cut(s) 13, 625
AsuHPI GGTGA 3 cut(s) 213, 267, 394
AsuII TTCGAA 1 cut(s) 69
AvaI CYCGRG 2 cut(s) 159, 452
BaeGI GKGCMC 2 cut(s) 49, 131
BanII GRGCYC 1 cut(s) 166
BarI GAAGNNNNNNTAC 2 cut(s) 69, 101
BauI CACGAG 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 166
BccI CCATC 2 cut(s) 250, 572
BcgI CGANNNNNNTGC 4 cut(s) 433, 467, 566, 600
BcoDI GTCTC 4 cut(s) 160, 344, 455, 702
BfaI CTAG 1 cut(s) 168
BglI GCCNNNNNGGC 1 cut(s) 624
BglII AGATCT 1 cut(s) 358
BmeT110I CYCGRG 2 cut(s) 159, 452
BmsI GCATC 2 cut(s) 91, 543
Bpu14I TTCGAA 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 368
BsaJI CCNNGG 1 cut(s) 400
BsaWI WCCGGW 1 cut(s) 22
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 95
Bse1I ACTGG 1 cut(s) 278
BseAI TCCGGA 1 cut(s) 22
BseDI CCNNGG 1 cut(s) 400
BseGI GGATG 4 cut(s) 31, 106, 374, 728
BseLI CCNNNNNNNGG 2 cut(s) 22, 95
BseMII CTCAG 3 cut(s) 525, 611, 635
BseNI ACTGG 1 cut(s) 278
BseSI GKGCMC 2 cut(s) 49, 131
BseYI CCCAGC 1 cut(s) 779
BshFI GGCC 2 cut(s) 15, 627
BsiHKAI GWGCWC 1 cut(s) 166
BsiHKCI CYCGRG 2 cut(s) 159, 452
BsiSI CCGG 1 cut(s) 23
BslFI GGGAC 1 cut(s) 595
BslI CCNNNNNNNGG 2 cut(s) 22, 95
BsmAI GTCTC 4 cut(s) 160, 344, 455, 702
BsmFI GGGAC 1 cut(s) 595
BsmI GAATGC 1 cut(s) 620
BsnI GGCC 2 cut(s) 15, 627
BsoBI CYCGRG 2 cut(s) 159, 452
Bsp119I TTCGAA 1 cut(s) 69
Bsp1286I GDGCHC 3 cut(s) 49, 131, 166
Bsp13I TCCGGA 1 cut(s) 22
Bsp143I GATC 3 cut(s) 19, 116, 358
BspACI CCGC 1 cut(s) 74
BspANI GGCC 2 cut(s) 15, 627
BspCNI CTCAG 3 cut(s) 526, 612, 636
BspEI TCCGGA 1 cut(s) 22
BspHI TCATGA 2 cut(s) 235, 286
BspPI GGATC 1 cut(s) 14
BspT104I TTCGAA 1 cut(s) 69
BsrI ACTGG 1 cut(s) 278
BssECI CCNNGG 1 cut(s) 400
BssMI GATC 3 cut(s) 19, 116, 358
BssSI CACGAG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 805
BstBI TTCGAA 1 cut(s) 69
BstDEI CTNAG 4 cut(s) 507, 534, 620, 644
BstF5I GGATG 4 cut(s) 31, 106, 374, 728
BstKTI GATC 3 cut(s) 22, 119, 361
BstMAI GTCTC 4 cut(s) 160, 344, 455, 702
BstMBI GATC 3 cut(s) 19, 116, 358
BstMWI GCNNNNNNNGC 3 cut(s) 624, 675, 684
BstNSI RCATGY 1 cut(s) 605
BstSLI GKGCMC 2 cut(s) 49, 131
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BsuRI GGCC 2 cut(s) 15, 627
BtsCI GGATG 4 cut(s) 31, 106, 374, 728
CciI TCATGA 2 cut(s) 235, 286
Csp6I GTAC 1 cut(s) 57
CviAII CATG 6 cut(s) 53, 236, 287, 323, 602, 795
CviJI RGCY 5 cut(s) 15, 164, 258, 568, 627
CviKI_1 RGCY 5 cut(s) 15, 164, 258, 568, 627
CviQI GTAC 1 cut(s) 57
DdeI CTNAG 4 cut(s) 507, 534, 620, 644
DpnI GATC 3 cut(s) 21, 118, 360
DpnII GATC 3 cut(s) 19, 116, 358
Ecl136II GAGCTC 1 cut(s) 164
Eco24I GRGCYC 1 cut(s) 166
Eco32I GATATC 1 cut(s) 182
Eco53kI GAGCTC 1 cut(s) 164
Eco88I CYCGRG 2 cut(s) 159, 452
EcoICRI GAGCTC 1 cut(s) 164
EcoRV GATATC 1 cut(s) 182
EcoT38I GRGCYC 1 cut(s) 166
FaeI CATG 6 cut(s) 56, 239, 290, 326, 605, 798
FaqI GGGAC 1 cut(s) 595
FatI CATG 6 cut(s) 52, 235, 286, 322, 601, 794
FokI GGATG 4 cut(s) 38, 113, 381, 735
FriOI GRGCYC 1 cut(s) 166
FspBI CTAG 1 cut(s) 168
GsaI CCCAGC 1 cut(s) 783
HaeIII GGCC 2 cut(s) 15, 627
HapII CCGG 1 cut(s) 23
Hin1II CATG 6 cut(s) 56, 239, 290, 326, 605, 798
HindIII AAGCTT 1 cut(s) 566
HpaII CCGG 1 cut(s) 23
HphI GGTGA 3 cut(s) 213, 267, 394
Hpy166II GTNNAC 1 cut(s) 320
Hpy188I TCNGA 3 cut(s) 247, 404, 645
Hpy8I GTNNAC 1 cut(s) 320
HpyAV CCTTC 2 cut(s) 359, 680
HpyCH4III ACNGT 1 cut(s) 805
HpyCH4V TGCA 4 cut(s) 104, 444, 654, 767
HpyF10VI GCNNNNNNNGC 3 cut(s) 624, 675, 684
HpyF3I CTNAG 4 cut(s) 507, 534, 620, 644
Hsp92II CATG 6 cut(s) 56, 239, 290, 326, 605, 798
Kpn2I TCCGGA 1 cut(s) 22
Kzo9I GATC 3 cut(s) 19, 116, 358
LmnI GCTCC 1 cut(s) 169
LweI GCATC 2 cut(s) 91, 543
MaeI CTAG 1 cut(s) 168
MaeIII GTNAC 1 cut(s) 186
MalI GATC 3 cut(s) 21, 118, 360
MboI GATC 3 cut(s) 19, 116, 358
MboII GAAGA 1 cut(s) 210
MfeI CAATTG 1 cut(s) 609
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 3 cut(s) 49, 131, 166
MluCI AATT 4 cut(s) 498, 609, 768, 831
MlyI GAGTC 3 cut(s) 457, 466, 704
MnlI CCTC 3 cut(s) 338, 410, 747
MroI TCCGGA 1 cut(s) 22
MseI TTAA 4 cut(s) 435, 497, 522, 711
MslI CAYNNNNRTG 1 cut(s) 195
MspI CCGG 1 cut(s) 23
MunI CAATTG 1 cut(s) 609
Mva1269I GAATGC 1 cut(s) 620
MwoI GCNNNNNNNGC 3 cut(s) 624, 675, 684
NdeII GATC 3 cut(s) 19, 116, 358
NlaIII CATG 6 cut(s) 56, 239, 290, 326, 605, 798
NspI RCATGY 1 cut(s) 605
NspV TTCGAA 1 cut(s) 69
PaeR7I CTCGAG 2 cut(s) 159, 452
PagI TCATGA 2 cut(s) 235, 286
PciI ACATGT 1 cut(s) 601
PctI GAATGC 1 cut(s) 620
PfeI GAWTC 7 cut(s) 143, 203, 395, 430, 482, 529, 640
PflMI CCANNNNNTGG 1 cut(s) 95
PleI GAGTC 3 cut(s) 456, 465, 703
PpsI GAGTC 3 cut(s) 456, 465, 703
PscI ACATGT 1 cut(s) 601
Psp124BI GAGCTC 1 cut(s) 166
PspFI CCCAGC 1 cut(s) 779
PsuI RGATCY 1 cut(s) 358
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
RseI CAYNNNNRTG 1 cut(s) 195
SacI GAGCTC 1 cut(s) 166
SaqAI TTAA 4 cut(s) 435, 497, 522, 711
Sau3AI GATC 3 cut(s) 19, 116, 358
SchI GAGTC 3 cut(s) 457, 466, 704
SduI GDGCHC 3 cut(s) 49, 131, 166
SetI ASST 6 cut(s) 141, 166, 330, 570, 683, 711
SfaNI GCATC 2 cut(s) 91, 543
Sfr274I CTCGAG 2 cut(s) 159, 452
SfuI TTCGAA 1 cut(s) 69
SlaI CTCGAG 2 cut(s) 159, 452
SmiMI CAYNNNNRTG 1 cut(s) 195
SmlI CTYRAG 3 cut(s) 159, 347, 452
SmoI CTYRAG 3 cut(s) 159, 347, 452
Sse9I AATT 4 cut(s) 498, 609, 768, 831
SsiI CCGC 1 cut(s) 74
SspI AATATT 1 cut(s) 788
SspMI CTAG 1 cut(s) 168
SstI GAGCTC 1 cut(s) 166
TaaI ACNGT 1 cut(s) 805
TaqI TCGA 5 cut(s) 39, 69, 160, 453, 576
TasI AATT 4 cut(s) 498, 609, 768, 831
TfiI GAWTC 7 cut(s) 143, 203, 395, 430, 482, 529, 640
Tru1I TTAA 4 cut(s) 435, 497, 522, 711
Tru9I TTAA 4 cut(s) 435, 497, 522, 711
TspDTI ATGAA 3 cut(s) 41, 216, 408
Van91I CCANNNNNTGG 1 cut(s) 95
XceI RCATGY 1 cut(s) 605
XhoI CTCGAG 2 cut(s) 159, 452
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.