RLG00000035377

Protein of unknown function (DUF760)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003449
Physical Location & Seq
Forward (+)
66057679 .. 66060093
2415 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035377

Sequence Viewer

Length: 558 bp
ATGGGCTCAACTCCGGTGATTCCAAACCTCCCATCCCAACGGCACTGTGGTACTCTTTCCATTATTGATTCACACTGTGTGCTTTATGGTCACAGTGTCCACAGTTGCCGAAAATCTTCACAACTTATGTACAGTATCATGATGATTGGATATATGTTTCAAAATGCACAGTACAGGTTGGAACTGCAGCAAAGTTTAGAGCCAGTTGCTCTTCCCGAAGGGCAAGAGAAAAAGGATGCACCAGAGTATGCACCTGGTACACAGAAGTCGAATGTGTCAGGGGAAGTTATTAGGTGGAATAATGTTTCTGGCCCTGAGAAAATAGATGCTAAGAAGTACATAGAGTTACTTGAAGCAGAGATTGAGGAATTGAATAATGAAGTTGGTAGGAAATCTGCAAATGGACAAAATGAATTGTTGGAGTATCTCAAGTCTCTCGAGCCCCAAAATTTAAAGGAGTTGACTAGTACTGCTGGAGACGATGTTGTGGTTGCAATGGATACATTCATCAAGCGGCTTTTGGCTGTTTCAGATCCTAGCCAAATGAAGGCTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF760

Protein Analysis

186

Amino Acids

20.56

Weight (kDa)

5.27

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF760 PF05542 40 - 66 8.1e-07 Protein of unknown function (DUF760)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 514
AclWI GGATC 1 cut(s) 527
AcsI RAATTY 1 cut(s) 448
AdeI CACNNNGTG 2 cut(s) 77, 79
AfaI GTAC 6 cut(s) 52, 131, 173, 259, 338, 469
AfiI CCNNNNNNNGG 1 cut(s) 547
AgsI TTSAA 3 cut(s) 161, 353, 373
AhlI ACTAGT 1 cut(s) 464
AjnI CCWGG 1 cut(s) 253
AluBI AGCT 1 cut(s) 555
AluI AGCT 1 cut(s) 555
Alw26I GTCTC 2 cut(s) 438, 471
AlwI GGATC 1 cut(s) 527
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 1 cut(s) 310
ApeKI GCWGC 1 cut(s) 187
ApoI RAATTY 1 cut(s) 448
Asp700I GAANNNNTTC 1 cut(s) 115
AspS9I GGNCC 1 cut(s) 311
AsuHPI GGTGA 1 cut(s) 28
AsuNHI GCTAGC 1 cut(s) 551
AvaI CYCGRG 1 cut(s) 437
BanII GRGCYC 2 cut(s) 8, 444
BbvI GCAGC 1 cut(s) 199
BccI CCATC 1 cut(s) 40
BceAI ACGGC 1 cut(s) 56
BciT130I CCWGG 1 cut(s) 255
BciVI GTATCC 1 cut(s) 493
BcoDI GTCTC 2 cut(s) 438, 471
BcuI ACTAGT 1 cut(s) 464
BfaI CTAG 3 cut(s) 465, 537, 552
BfmI CTRYAG 1 cut(s) 185
BfuI GTATCC 1 cut(s) 493
BisI GCNGC 2 cut(s) 188, 515
BlsI GCNGC 2 cut(s) 189, 516
BmcAI AGTACT 1 cut(s) 469
Bme1390I CCNGG 1 cut(s) 255
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 311
BmrFI CCNGG 1 cut(s) 255
BmsI GCATC 2 cut(s) 226, 316
BmtI GCTAGC 1 cut(s) 555
BpmI CTGGAG 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 413
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 468, 498
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse1I ACTGG 1 cut(s) 203
Bse3DI GCAATG 1 cut(s) 501
BseBI CCWGG 1 cut(s) 255
BseGI GGATG 2 cut(s) 32, 241
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 1 cut(s) 306
BseNI ACTGG 1 cut(s) 203
BseXI GCAGC 1 cut(s) 199
BshFI GGCC 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 1 cut(s) 14
BslI CCNNNNNNNGG 1 cut(s) 547
BsmAI GTCTC 2 cut(s) 438, 471
BsmBI CGTCTC 1 cut(s) 471
BsnI GGCC 1 cut(s) 312
BsoBI CYCGRG 1 cut(s) 437
Bsp1286I GDGCHC 2 cut(s) 8, 444
Bsp1407I TGTACA 1 cut(s) 129
Bsp143I GATC 1 cut(s) 532
BspACI CCGC 1 cut(s) 514
BspANI GGCC 1 cut(s) 312
BspCNI CTCAG 1 cut(s) 307
BspHI TCATGA 1 cut(s) 138
BspMAI CTGCAG 1 cut(s) 189
BspOI GCTAGC 1 cut(s) 555
BspPI GGATC 1 cut(s) 527
BspQI GCTCTTC 1 cut(s) 216
BsrDI GCAATG 1 cut(s) 501
BsrGI TGTACA 1 cut(s) 129
BsrI ACTGG 1 cut(s) 203
BssMI GATC 1 cut(s) 532
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 6 cut(s) 47, 77, 95, 104, 134, 171
Bst6I CTCTTC 1 cut(s) 216
BstAUI TGTACA 1 cut(s) 129
BstC8I GCNNGC 1 cut(s) 553
BstDEI CTNAG 2 cut(s) 315, 330
BstF5I GGATG 2 cut(s) 32, 241
BstKTI GATC 1 cut(s) 535
BstMAI GTCTC 2 cut(s) 438, 471
BstMBI GATC 1 cut(s) 532
BstNI CCWGG 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 253
BstSFI CTRYAG 1 cut(s) 185
BstV1I GCAGC 1 cut(s) 199
BstX2I RGATCY 1 cut(s) 532
BstYI RGATCY 1 cut(s) 532
BsuI GTATCC 1 cut(s) 493
BsuRI GGCC 1 cut(s) 312
BtsCI GGATG 2 cut(s) 32, 241
BtsIMutI CAGTG 3 cut(s) 43, 73, 100
Cac8I GCNNGC 1 cut(s) 553
CciI TCATGA 1 cut(s) 138
Cfr13I GGNCC 1 cut(s) 311
CsiI ACCWGGT 1 cut(s) 253
Csp6I GTAC 6 cut(s) 51, 130, 172, 258, 337, 468
CviAII CATG 1 cut(s) 139
CviJI RGCY 9 cut(s) 6, 202, 312, 442, 517, 524, 540, 551, 555
CviKI_1 RGCY 9 cut(s) 6, 202, 312, 442, 517, 524, 540, 551, 555
CviQI GTAC 6 cut(s) 51, 130, 172, 258, 337, 468
DdeI CTNAG 2 cut(s) 315, 330
DpnI GATC 1 cut(s) 534
DpnII GATC 1 cut(s) 532
DraI TTTAAA 1 cut(s) 453
DraIII CACNNNGTG 2 cut(s) 77, 79
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco24I GRGCYC 2 cut(s) 8, 444
Eco88I CYCGRG 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 253
EcoT38I GRGCYC 2 cut(s) 8, 444
Esp3I CGTCTC 1 cut(s) 471
FaeI CATG 1 cut(s) 142
FaiI YATR 7 cut(s) 87, 128, 140, 153, 155, 249, 341
FatI CATG 1 cut(s) 138
Fnu4HI GCNGC 2 cut(s) 188, 515
FokI GGATG 2 cut(s) 19, 248
FriOI GRGCYC 2 cut(s) 8, 444
Fsp4HI GCNGC 2 cut(s) 188, 515
FspBI CTAG 3 cut(s) 465, 537, 552
GluI GCNGC 2 cut(s) 188, 515
GsuI CTGGAG 1 cut(s) 495
HaeIII GGCC 1 cut(s) 312
HapII CCGG 1 cut(s) 14
Hin1II CATG 1 cut(s) 142
HincII GTYRAC 1 cut(s) 462
HindII GTYRAC 1 cut(s) 462
HinfI GANTC 2 cut(s) 19, 68
HpaII CCGG 1 cut(s) 14
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 3 cut(s) 100, 260, 462
Hpy188I TCNGA 1 cut(s) 532
Hpy188III TCNNGA 3 cut(s) 139, 215, 437
Hpy8I GTNNAC 3 cut(s) 100, 260, 462
HpyAV CCTTC 2 cut(s) 212, 541
HpyCH4III ACNGT 6 cut(s) 47, 77, 95, 104, 134, 171
HpyCH4V TGCA 6 cut(s) 167, 187, 239, 251, 398, 494
HpyF3I CTNAG 2 cut(s) 315, 330
Hsp92II CATG 1 cut(s) 142
Kzo9I GATC 1 cut(s) 532
LguI GCTCTTC 1 cut(s) 216
Lsp1109I GCAGC 1 cut(s) 199
LweI GCATC 2 cut(s) 226, 316
MabI ACCWGGT 1 cut(s) 253
MaeI CTAG 3 cut(s) 465, 537, 552
MaeIII GTNAC 2 cut(s) 89, 345
MalI GATC 1 cut(s) 534
MboI GATC 1 cut(s) 532
MboII GAAGA 2 cut(s) 108, 203
MflI RGATCY 1 cut(s) 532
MhlI GDGCHC 2 cut(s) 8, 444
MluCI AATT 3 cut(s) 368, 413, 448
MmeI TCCRAC 2 cut(s) 159, 399
MnlI CCTC 2 cut(s) 38, 358
MroXI GAANNNNTTC 1 cut(s) 115
MseI TTAA 1 cut(s) 452
MspI CCGG 1 cut(s) 14
MspR9I CCNGG 1 cut(s) 255
MvaI CCWGG 1 cut(s) 255
NdeII GATC 1 cut(s) 532
NheI GCTAGC 1 cut(s) 551
NlaIII CATG 1 cut(s) 142
NmuCI GTSAC 1 cut(s) 89
PaeR7I CTCGAG 1 cut(s) 437
PagI TCATGA 1 cut(s) 138
PciSI GCTCTTC 1 cut(s) 216
PdmI GAANNNNTTC 1 cut(s) 115
PfeI GAWTC 2 cut(s) 19, 68
PkrI GCNGC 2 cut(s) 189, 516
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
PspPI GGNCC 1 cut(s) 311
PstI CTGCAG 1 cut(s) 189
PsuI RGATCY 1 cut(s) 532
RsaI GTAC 6 cut(s) 52, 131, 173, 259, 338, 469
RsaNI GTAC 6 cut(s) 51, 130, 172, 258, 337, 468
SapI GCTCTTC 1 cut(s) 216
SaqAI TTAA 1 cut(s) 452
SatI GCNGC 2 cut(s) 188, 515
Sau3AI GATC 1 cut(s) 532
Sau96I GGNCC 1 cut(s) 311
ScaI AGTACT 1 cut(s) 469
ScrFI CCNGG 1 cut(s) 255
SduI GDGCHC 2 cut(s) 8, 444
SetI ASST 5 cut(s) 30, 179, 256, 296, 557
SexAI ACCWGGT 1 cut(s) 253
SfaNI GCATC 2 cut(s) 226, 316
SfcI CTRYAG 1 cut(s) 185
Sfr274I CTCGAG 1 cut(s) 437
SlaI CTCGAG 1 cut(s) 437
SmlI CTYRAG 2 cut(s) 428, 437
SmoI CTYRAG 2 cut(s) 428, 437
SpeI ACTAGT 1 cut(s) 464
Sse9I AATT 3 cut(s) 368, 413, 448
SsiI CCGC 1 cut(s) 514
SspMI CTAG 3 cut(s) 465, 537, 552
StyD4I CCNGG 1 cut(s) 253
TaaI ACNGT 6 cut(s) 47, 77, 95, 104, 134, 171
TaqI TCGA 2 cut(s) 269, 438
TasI AATT 3 cut(s) 368, 413, 448
TatI WGTACW 4 cut(s) 129, 171, 336, 467
TauI GCSGC 1 cut(s) 517
TfiI GAWTC 2 cut(s) 19, 68
Tru1I TTAA 1 cut(s) 452
Tru9I TTAA 1 cut(s) 452
TscAI CASTG 3 cut(s) 50, 80, 100
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 1 cut(s) 187
Tsp45I GTSAC 1 cut(s) 89
TspDTI ATGAA 3 cut(s) 393, 426, 496
TspRI CASTG 3 cut(s) 50, 80, 100
XapI RAATTY 1 cut(s) 448
XcmI CCANNNNNNNNNTGG 1 cut(s) 44
XhoI CTCGAG 1 cut(s) 437
XmnI GAANNNNTTC 1 cut(s) 115
XspI CTAG 3 cut(s) 465, 537, 552
ZrmI AGTACT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.