Rorug06G0328800

Protein of unknown function (DUF760)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003449
Physical Location & Seq
Forward (+)
48184827 .. 48186997
2171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0328800.1

Sequence Viewer

Length: 1524 bp
ATGTTGCTAAGAAACCTGCGAAGCTCCATCGCCGCCGTCCGCCGTTTCTCGGTGGTGGCGAAGGTGGCGAGCACCGGAGCGACGAAGGAATCGGGCGGTGGAAGAGAGACGCTGGGAAGGAGACTTCTGGGCCTGGTGTATGCAAAACGCAGCGCGGCGGTTACTATAGGGAAATGGAAAGAGGAAGGAGGCACAGTGCGGAAGTATGAGCTTAATCGGATTGTTCGTGAGCTTCGTAAGCTCCGGCGTTATAAGCACGCTCTTGAGATATGCGAATGGATGACTTTACAGAAAGATATCAAGCTACTGGCAGGTGATTATGCTGTTCAGTTGGATTTGATTGCAAAGGTTCGTGGTATGAATAGCGCAGAGAAGTTTTTCGAGGATCTCCCTGAGCAGATGAGAGACGGCACGACCTGTTCAGCTCTTCTGCATACATATGTCCAGAATAAAGATGCTGCCAAAGCGGAAGCTCTGGTGGAGAAGATGAAAGAGTGTGGTTTCGTGAAGAATCCCCTTCCTTACAACCACATGCTGTCTTTGTATGTGTCCGATGGGCAATTAGACAAGATTCCAGAAGTAATTGGGGAGATGAAGGTTCATACTAAACCGGATGTTGTCACTTACAATCTATGGTTAACAGTTTGTGCTTCGCAAAACAATGTTGATAGTGCAGAAAGAGTGTTTCTTGAATTGAAGAAGGCAAAGGTAAATCCGGACTGGGTGACATTTAGCACGCTAACAAACTTGTACATCAAAAGTTCACTCATTGAAAAAGCAGCAGCTACCTTGAAGGAGATGGAGAAAAGGGCATCTCGGAAGCATCGAGTCGCATATTCATCTCTCCTCAGCCTGCATGCAAACATTGGGGACAAGGCTGGTGTTCAGCGAGTTTGGAAGAAACTAAAATCATGTTTCCCCAAAATGAATGATGCTGAGTATACATGTATGATAGCTTCGCTTGTGAAACTCAAGGAGATTGAAGAAGCTGAGAAACTCTATACTGAGTGGGAGTCTGTTTCTGGGACTCATGATCCTAGGGTCTCAAATATAATTCTTGCAGCTTACATCAACAGAAACCAAATGGATGTGGCTGAAACCTTCTGTAGTCGTATGGTGCAAAATAGCATCACCCCCTGTTACAGTACTTGGGAGCTTCTTACGTGGGGCTATTTGAAACAAAAGTATACGAAAAAAGTGCTAGAGTATTTTAAGAAAGCAGTTGGTAGTGTGAAAAAATGGGATCCCGACCAAAGATTGATTGCAGAAGTATTTGGCCGGCTCAATGAGGAAGGCAGTGTTGAAGAGGCAGAGGAACTAGTGGTTTTCCTTCGGAATGCTGGTCATGTGAGTACTGAGACATATAATTCACTTCTACGAACTTATGCTCAAGCTGGTACAATGCCACTTTTGGTTGAAGAACGGATGAAGAAGGACAATGTTGAATTGAATAAGGAGACTCGCTCGCTTATAAAAAGGATCAGCAAAATTTGTCCGAGTGAAGTTCCTAGCCTATGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF760

Protein Analysis

507

Amino Acids

57.61

Weight (kDa)

9.26

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 174 - 216 7.2e-06 Pentatricopeptide repeat domain
PPR_3 PF13812 203 - 251 3e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 204 - 249 3.8e-10 PPR repeat family
PPR_long PF17177 213 - 303 7.7e-06 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 252, 1472
AarI CACCTGC 1 cut(s) 302
Acc36I ACCTGC 2 cut(s) 24, 302
AccI GTMKAC 2 cut(s) 941, 1187
AccII CGCG 1 cut(s) 155
AccIII TCCGGA 1 cut(s) 715
AciI CCGC 7 cut(s) 33, 40, 96, 155, 158, 199, 467
AclWI GGATC 5 cut(s) 393, 1028, 1238, 1251, 1487
AcoI YGGCCR 1 cut(s) 1276
AcsI RAATTY 1 cut(s) 1488
AfaI GTAC 4 cut(s) 752, 1147, 1354, 1399
AfiI CCNNNNNNNGG 1 cut(s) 49
AflIII ACRYGT 1 cut(s) 944
AhlI ACTAGT 1 cut(s) 1318
AjnI CCWGG 1 cut(s) 132
Alw21I GWGCWC 1 cut(s) 74
Alw26I GTCTC 6 cut(s) 101, 115, 399, 1048, 1352, 1451
AlwI GGATC 5 cut(s) 393, 1028, 1238, 1251, 1487
Aor13HI TCCGGA 1 cut(s) 715
AoxI GGCC 2 cut(s) 130, 1276
ApeKI GCWGC 5 cut(s) 150, 458, 779, 782, 1061
ApoI RAATTY 1 cut(s) 1488
Asp700I GAANNNNTTC 1 cut(s) 377
AspA2I CCTAGG 1 cut(s) 1037
AspLEI GCGC 2 cut(s) 155, 368
AspS9I GGNCC 1 cut(s) 130
AsuHPI GGTGA 3 cut(s) 326, 736, 1123
AvrII CCTAGG 1 cut(s) 1037
BamHI GGATCC 1 cut(s) 1243
BarI GAAGNNNNNNTAC 2 cut(s) 940, 972
Bbv12I GWGCWC 1 cut(s) 74
BbvCI CCTCAGC 1 cut(s) 848
BbvI GCAGC 5 cut(s) 162, 445, 791, 794, 1073
BccI CCATC 3 cut(s) 35, 548, 793
BceAI ACGGC 3 cut(s) 20, 27, 424
BcgI CGANNNNNNTGC 2 cut(s) 1180, 1214
BciT130I CCWGG 1 cut(s) 134
BcoDI GTCTC 6 cut(s) 101, 115, 399, 1048, 1352, 1451
BcuI ACTAGT 1 cut(s) 1318
BfaI CTAG 4 cut(s) 1038, 1202, 1319, 1509
BfmI CTRYAG 2 cut(s) 165, 1105
BfuAI ACCTGC 2 cut(s) 24, 302
BisI GCNGC 7 cut(s) 33, 151, 156, 459, 780, 783, 1062
BlnI CCTAGG 1 cut(s) 1037
BlsI GCNGC 7 cut(s) 34, 152, 157, 460, 781, 784, 1063
BmcAI AGTACT 2 cut(s) 1147, 1354
Bme1390I CCNGG 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 130
BmiI GGNNCC 1 cut(s) 1245
BmrFI CCNGG 1 cut(s) 134
BmrI ACTGGG 1 cut(s) 730
BmsI GCATC 5 cut(s) 445, 821, 832, 922, 1137
BmuI ACTGGG 1 cut(s) 730
BplI GAGNNNNNCTC 2 cut(s) 1448, 1480
Bpu10I CCTNAGC 2 cut(s) 393, 848
BpuEI CTTGAG 3 cut(s) 284, 956, 1374
BsaAI YACGTR 1 cut(s) 1164
BsaI GGTCTC 1 cut(s) 1048
BsaJI CCNNGG 1 cut(s) 1037
BsaWI WCCGGW 3 cut(s) 74, 610, 715
BsaXI ACNNNNNCTCC 2 cut(s) 180, 210
Bsc4I CCNNNNNNNGG 1 cut(s) 49
Bse118I RCCGGY 1 cut(s) 1278
Bse1I ACTGG 2 cut(s) 312, 725
BseAI TCCGGA 1 cut(s) 715
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 1 cut(s) 1037
BseGI GGATG 4 cut(s) 285, 619, 1093, 1431
BseLI CCNNNNNNNGG 1 cut(s) 49
BseMII CTCAG 6 cut(s) 384, 862, 927, 981, 996, 1347
BseNI ACTGG 2 cut(s) 312, 725
BseRI GAGGAG 1 cut(s) 836
BseXI GCAGC 5 cut(s) 162, 445, 791, 794, 1073
BseYI CCCAGC 1 cut(s) 112
BsgI GTGCAG 1 cut(s) 693
Bsh1236I CGCG 1 cut(s) 155
BshFI GGCC 2 cut(s) 132, 1278
BsiHKAI GWGCWC 1 cut(s) 74
BsiSI CCGG 5 cut(s) 75, 244, 611, 716, 1279
BslFI GGGAC 2 cut(s) 884, 1039
BslI CCNNNNNNNGG 1 cut(s) 49
BsmAI GTCTC 6 cut(s) 101, 115, 399, 1048, 1352, 1451
BsmBI CGTCTC 2 cut(s) 101, 399
BsmFI GGGAC 2 cut(s) 884, 1039
BsmI GAATGC 1 cut(s) 1342
BsnI GGCC 2 cut(s) 132, 1278
Bso31I GGTCTC 1 cut(s) 1048
Bsp1286I GDGCHC 1 cut(s) 74
Bsp13I TCCGGA 1 cut(s) 715
Bsp1407I TGTACA 1 cut(s) 750
Bsp143I GATC 4 cut(s) 385, 1033, 1243, 1479
BspACI CCGC 7 cut(s) 33, 40, 96, 155, 158, 199, 467
BspANI GGCC 2 cut(s) 132, 1278
BspCNI CTCAG 6 cut(s) 385, 861, 928, 982, 997, 1348
BspEI TCCGGA 1 cut(s) 715
BspFNI CGCG 1 cut(s) 155
BspHI TCATGA 1 cut(s) 1030
BspLI GGNNCC 1 cut(s) 1245
BspMI ACCTGC 2 cut(s) 24, 302
BspPI GGATC 5 cut(s) 393, 1028, 1238, 1251, 1487
BspQI GCTCTTC 1 cut(s) 432
BspTNI GGTCTC 1 cut(s) 1048
BsrFI RCCGGY 1 cut(s) 1278
BsrGI TGTACA 1 cut(s) 750
BsrI ACTGG 2 cut(s) 312, 725
BssAI RCCGGY 1 cut(s) 1278
BssECI CCNNGG 1 cut(s) 1037
BssMI GATC 4 cut(s) 385, 1033, 1243, 1479
BssNAI GTATAC 2 cut(s) 942, 1188
BssT1I CCWWGG 1 cut(s) 1037
Bst1107I GTATAC 2 cut(s) 942, 1188
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 3 cut(s) 196, 643, 1145
Bst6I CTCTTC 3 cut(s) 97, 432, 1299
BstAUI TGTACA 1 cut(s) 750
BstBAI YACGTR 1 cut(s) 1164
BstC8I GCNNGC 7 cut(s) 70, 258, 737, 854, 858, 1280, 1466
BstDEI CTNAG 7 cut(s) 8, 393, 848, 936, 990, 1005, 1356
BstF5I GGATG 4 cut(s) 285, 619, 1093, 1431
BstFNI CGCG 1 cut(s) 155
BstHHI GCGC 2 cut(s) 155, 368
BstKTI GATC 4 cut(s) 388, 1036, 1246, 1482
BstMAI GTCTC 6 cut(s) 101, 115, 399, 1048, 1352, 1451
BstMBI GATC 4 cut(s) 385, 1033, 1243, 1479
BstMWI GCNNNNNNNGC 4 cut(s) 65, 238, 253, 464
BstNI CCWGG 1 cut(s) 134
BstNSI RCATGY 3 cut(s) 535, 860, 948
BstSCI CCNGG 1 cut(s) 132
BstSFI CTRYAG 2 cut(s) 165, 1105
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 5 cut(s) 162, 445, 791, 794, 1073
BstX2I RGATCY 2 cut(s) 385, 1243
BstYI RGATCY 2 cut(s) 385, 1243
BstZ17I GTATAC 2 cut(s) 942, 1188
BsuRI GGCC 2 cut(s) 132, 1278
BtgZI GCGATG 1 cut(s) 13
BtsCI GGATG 4 cut(s) 285, 619, 1093, 1431
BtsI GCAGTG 1 cut(s) 1303
BtsIMutI CAGTG 2 cut(s) 201, 1303
BveI ACCTGC 2 cut(s) 24, 302
Cac8I GCNNGC 7 cut(s) 70, 258, 737, 854, 858, 1280, 1466
CciI TCATGA 1 cut(s) 1030
CfoI GCGC 2 cut(s) 155, 368
Cfr10I RCCGGY 1 cut(s) 1278
Cfr13I GGNCC 1 cut(s) 130
CseI GACGC 1 cut(s) 118
Csp6I GTAC 4 cut(s) 751, 1146, 1353, 1398
CviAII CATG 6 cut(s) 532, 857, 912, 945, 1031, 1346
CviQI GTAC 4 cut(s) 751, 1146, 1353, 1398
DdeI CTNAG 7 cut(s) 8, 393, 848, 936, 990, 1005, 1356
DpnI GATC 4 cut(s) 387, 1035, 1245, 1481
DpnII GATC 4 cut(s) 385, 1033, 1243, 1479
EaeI YGGCCR 1 cut(s) 1276
Eam1104I CTCTTC 3 cut(s) 97, 432, 1299
EarI CTCTTC 3 cut(s) 97, 432, 1299
EciI GGCGGA 1 cut(s) 29
Eco130I CCWWGG 1 cut(s) 1037
Eco31I GGTCTC 1 cut(s) 1048
Eco32I GATATC 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 132
EcoRV GATATC 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 1037
ErhI CCWWGG 1 cut(s) 1037
Esp3I CGTCTC 2 cut(s) 101, 399
FaeI CATG 6 cut(s) 535, 860, 915, 948, 1034, 1349
FaqI GGGAC 2 cut(s) 884, 1039
FatI CATG 6 cut(s) 531, 856, 911, 944, 1030, 1345
FauNDI CATATG 1 cut(s) 439
FblI GTMKAC 2 cut(s) 941, 1187
Fnu4HI GCNGC 7 cut(s) 33, 151, 156, 459, 780, 783, 1062
FokI GGATG 4 cut(s) 292, 626, 1100, 1438
Fsp4HI GCNGC 7 cut(s) 33, 151, 156, 459, 780, 783, 1062
FspBI CTAG 4 cut(s) 1038, 1202, 1319, 1509
GlaI GCGC 2 cut(s) 154, 367
GluI GCNGC 7 cut(s) 33, 151, 156, 459, 780, 783, 1062
GsaI CCCAGC 1 cut(s) 116
HaeIII GGCC 2 cut(s) 132, 1278
HapII CCGG 5 cut(s) 75, 244, 611, 716, 1279
HgaI GACGC 1 cut(s) 118
HhaI GCGC 2 cut(s) 155, 368
Hin1II CATG 6 cut(s) 535, 860, 915, 948, 1034, 1349
Hin6I GCGC 2 cut(s) 153, 366
HinP1I GCGC 2 cut(s) 153, 366
HincII GTYRAC 1 cut(s) 639
HindII GTYRAC 1 cut(s) 639
HinfI GANTC 7 cut(s) 89, 511, 571, 828, 1013, 1027, 1459
HpaI GTTAAC 1 cut(s) 639
HpaII CCGG 5 cut(s) 75, 244, 611, 716, 1279
HphI GGTGA 3 cut(s) 326, 736, 1123
Hpy166II GTNNAC 4 cut(s) 639, 764, 942, 1188
Hpy188I TCNGA 5 cut(s) 219, 553, 819, 1335, 1497
Hpy188III TCNNGA 9 cut(s) 227, 263, 445, 505, 575, 689, 716, 1031, 1247
Hpy8I GTNNAC 4 cut(s) 639, 764, 942, 1188
Hpy99I CGWCG 1 cut(s) 85
HpyCH4III ACNGT 3 cut(s) 196, 643, 1145
HpyCH4IV ACGT 1 cut(s) 1163
HpyCH4V TGCA 9 cut(s) 143, 344, 433, 674, 856, 860, 1061, 1120, 1265
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 238, 253, 464
HpyF3I CTNAG 7 cut(s) 8, 393, 848, 936, 990, 1005, 1356
HpySE526I ACGT 1 cut(s) 1163
Hsp92II CATG 6 cut(s) 535, 860, 915, 948, 1034, 1349
HspAI GCGC 2 cut(s) 153, 366
Kpn2I TCCGGA 1 cut(s) 715
KroI GCCGGC 1 cut(s) 1278
KroNI GCCGGC 1 cut(s) 1280
KspAI GTTAAC 1 cut(s) 639
Kzo9I GATC 4 cut(s) 385, 1033, 1243, 1479
LguI GCTCTTC 1 cut(s) 432
LmnI GCTCC 4 cut(s) 29, 77, 246, 1153
Lsp1109I GCAGC 5 cut(s) 162, 445, 791, 794, 1073
LweI GCATC 5 cut(s) 445, 821, 832, 922, 1137
MaeI CTAG 4 cut(s) 1038, 1202, 1319, 1509
MaeII ACGT 1 cut(s) 1163
MaeIII GTNAC 4 cut(s) 160, 619, 724, 1139
MalI GATC 4 cut(s) 387, 1035, 1245, 1481
MboI GATC 4 cut(s) 385, 1033, 1243, 1479
MflI RGATCY 2 cut(s) 385, 1243
MhlI GDGCHC 1 cut(s) 74
MluCI AATT 7 cut(s) 560, 582, 692, 1053, 1366, 1445, 1488
MlyI GAGTC 4 cut(s) 837, 1021, 1022, 1453
MmeI TCCRAC 1 cut(s) 312
MnlI CCTC 7 cut(s) 175, 182, 376, 857, 1282, 1300, 1306
MroI TCCGGA 1 cut(s) 715
MroNI GCCGGC 1 cut(s) 1278
MroXI GAANNNNTTC 1 cut(s) 377
MseI TTAA 4 cut(s) 213, 638, 1212, 1522
MslI CAYNNNNRTG 1 cut(s) 438
MspI CCGG 5 cut(s) 75, 244, 611, 716, 1279
MspR9I CCNGG 1 cut(s) 134
Mva1269I GAATGC 1 cut(s) 1342
MvaI CCWGG 1 cut(s) 134
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 4 cut(s) 65, 238, 253, 464
NaeI GCCGGC 1 cut(s) 1280
NdeI CATATG 1 cut(s) 439
NdeII GATC 4 cut(s) 385, 1033, 1243, 1479
NgoMIV GCCGGC 1 cut(s) 1278
NlaIII CATG 6 cut(s) 535, 860, 915, 948, 1034, 1349
NlaIV GGNNCC 1 cut(s) 1245
NmuCI GTSAC 2 cut(s) 619, 724
NspI RCATGY 3 cut(s) 535, 860, 948
PaeI GCATGC 1 cut(s) 860
PagI TCATGA 1 cut(s) 1030
PaqCI CACCTGC 1 cut(s) 302
PciI ACATGT 1 cut(s) 944
PciSI GCTCTTC 1 cut(s) 432
PcsI WCGNNNNNNNCGW 4 cut(s) 56, 223, 232, 823
PctI GAATGC 1 cut(s) 1342
PdiI GCCGGC 1 cut(s) 1280
PdmI GAANNNNTTC 1 cut(s) 377
PfeI GAWTC 3 cut(s) 89, 511, 571
PkrI GCNGC 7 cut(s) 34, 152, 157, 460, 781, 784, 1063
PleI GAGTC 4 cut(s) 836, 1021, 1021, 1453
PpsI GAGTC 4 cut(s) 836, 1021, 1021, 1453
Ppu21I YACGTR 1 cut(s) 1164
PscI ACATGT 1 cut(s) 944
PsiI TTATAA 2 cut(s) 252, 1472
Psp6I CCWGG 1 cut(s) 132
PspFI CCCAGC 1 cut(s) 112
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 1245
PspPI GGNCC 1 cut(s) 130
PsuI RGATCY 2 cut(s) 385, 1243
RsaI GTAC 4 cut(s) 752, 1147, 1354, 1399
RsaNI GTAC 4 cut(s) 751, 1146, 1353, 1398
RseI CAYNNNNRTG 1 cut(s) 438
SapI GCTCTTC 1 cut(s) 432
SaqAI TTAA 4 cut(s) 213, 638, 1212, 1522
SatI GCNGC 7 cut(s) 33, 151, 156, 459, 780, 783, 1062
Sau3AI GATC 4 cut(s) 385, 1033, 1243, 1479
Sau96I GGNCC 1 cut(s) 130
ScaI AGTACT 2 cut(s) 1147, 1354
SchI GAGTC 4 cut(s) 837, 1021, 1022, 1453
ScrFI CCNGG 1 cut(s) 134
SduI GDGCHC 1 cut(s) 74
SfaNI GCATC 5 cut(s) 445, 821, 832, 922, 1137
SfcI CTRYAG 2 cut(s) 165, 1105
SmiMI CAYNNNNRTG 1 cut(s) 438
SmlI CTYRAG 3 cut(s) 263, 971, 1389
SmoI CTYRAG 3 cut(s) 263, 971, 1389
SpeI ACTAGT 1 cut(s) 1318
SphI GCATGC 1 cut(s) 860
Sse9I AATT 7 cut(s) 560, 582, 692, 1053, 1366, 1445, 1488
SsiI CCGC 7 cut(s) 33, 40, 96, 155, 158, 199, 467
SspMI CTAG 4 cut(s) 1038, 1202, 1319, 1509
StyD4I CCNGG 1 cut(s) 132
StyI CCWWGG 1 cut(s) 1037
TaaI ACNGT 3 cut(s) 196, 643, 1145
TaiI ACGT 1 cut(s) 1166
TaqI TCGA 2 cut(s) 381, 826
TasI AATT 7 cut(s) 560, 582, 692, 1053, 1366, 1445, 1488
TatI WGTACW 3 cut(s) 750, 1145, 1352
TauI GCSGC 2 cut(s) 35, 158
TfiI GAWTC 3 cut(s) 89, 511, 571
Tru1I TTAA 4 cut(s) 213, 638, 1212, 1522
Tru9I TTAA 4 cut(s) 213, 638, 1212, 1522
TscAI CASTG 2 cut(s) 201, 1303
TseFI GTSAC 2 cut(s) 619, 724
TseI GCWGC 5 cut(s) 150, 458, 779, 782, 1061
Tsp45I GTSAC 2 cut(s) 619, 724
TspDTI ATGAA 7 cut(s) 374, 503, 590, 608, 828, 941, 1442
TspGWI ACGGA 1 cut(s) 1438
TspRI CASTG 2 cut(s) 201, 1303
XapI RAATTY 1 cut(s) 1488
XceI RCATGY 3 cut(s) 535, 860, 948
XmaJI CCTAGG 1 cut(s) 1037
XmiI GTMKAC 2 cut(s) 941, 1187
XmnI GAANNNNTTC 1 cut(s) 377
XspI CTAG 4 cut(s) 1038, 1202, 1319, 1509
ZrmI AGTACT 2 cut(s) 1147, 1354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.