Rmu_sc0000389.1_g000030

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000389.1
OG0002881
Physical Location & Seq
Forward (+)
134695 .. 137520
2826 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1380 bp
atgtctaaagcaccagctcaagaagaagcagtgcatgcttatggatgggctgctagagattcctcaggaattctttccccctaccatttcactagaagggctaacggggataatgatatcaccatcaagatacaatattgcggaatttgccatggagacattcacctcgtaaagaacaaagtatggtcgaccatatatccgactgtgcccgggattgttcatccttcgggtggctgtccagttgatccttgtgtggatctgtttacctttcgtcctgctttggcttttctggcttctactttggcgatggaagatgttctccgtttcggagatgatctgacatttctggcttcttgtgggtctggaatcgggatattccagtgctctcgtttgagttcgttgttgccttttttcaaacttttcgactctggcctgatggtgttcttcatacttggtgcgaattttattcgctttggcgctaccgctgtgacagcgcatgaaattgttggccaagtgaccaaggttgggctcaacgtcaaaaaattcaaagttggagatgtagccggagtagggtgcatgatgggttcatgtggctcatgcgagaactgcaaacaagacttggaaaattactgccccaaaatgttatggaccttcagtggacaatatgaagatggaacaagagcctttggtggatattcagataagtttgttgtggaagagcactttgccgtccgaatcccaaaagacattccactagcaggtgccgccccgctattgtgtgctgggattaccgtgtatagccccctgaaatatttcggactagtcccccagcccggtagcaagaaacatttgggtgttgttgggcttggaggtgtaggtcatatggccgtgaagtttgccaaagctttcggagcaaaggtcactgtgattagttcctctccgagtaaggaaaaggaagcagtggaacgacttggtgcagattcatttttgatcagtcatgaccatgagcaattggaggctgccttgggcaccatggatggtatcatcgacactgtttctgcttctcactctctcctcccattgattggcttgctgaaaactagtggaaaactagttttggtgggtgctccagtggagccacctgtgcttcccgtcttccctttaattatggggaggaaagttattgctggcagtgcaattgggggaatgaaagagactcaagagatgatagattttgcagcaaagcacaacatcacagcagatgtcgaagttattccaatggattatgtgaacactgctcttgaacgacttgagaagggcgacaatgttaaatatcgctttgtcatcgatgttgccaacacgataaaatctgcaaactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

459

Amino Acids

49.13

Weight (kDa)

6.67

Isoelectric Point (pI)

29.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 162 - 248 2.5e-13 Alcohol dehydrogenase GroES-like domain
2-Hacid_dh_C PF02826 280 - 323 9.9e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
AlaDh_PNT_C PF01262 284 - 354 3.7e-06 Alanine dehydrogenase/PNT, C-terminal domain
ADH_zinc_N PF00107 291 - 414 2e-22 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 749
Acc36I ACCTGC 1 cut(s) 749
AccB1I GGYRCC 2 cut(s) 761, 1028
AccB7I CCANNNNNTGG 1 cut(s) 1085
AccI GTMKAC 1 cut(s) 188
AciI CCGC 4 cut(s) 141, 483, 765, 770
AclWI GGATC 2 cut(s) 239, 264
AcoI YGGCCR 2 cut(s) 508, 885
AcsI RAATTY 4 cut(s) 69, 144, 460, 542
AcuI CTGAAG 1 cut(s) 637
AfiI CCNNNNNNNGG 6 cut(s) 230, 525, 570, 758, 833, 1085
AgsI TTSAA 3 cut(s) 415, 547, 1304
AhlI ACTAGT 3 cut(s) 820, 1100, 1111
AluBI AGCT 2 cut(s) 17, 905
AluI AGCT 2 cut(s) 17, 905
Alw21I GWGCWC 3 cut(s) 386, 723, 1129
Alw26I GTCTC 2 cut(s) 150, 1208
AlwI GGATC 2 cut(s) 239, 264
Ama87I CYCGRG 1 cut(s) 209
AoxI GGCC 3 cut(s) 430, 508, 885
ApeKI GCWGC 3 cut(s) 50, 1019, 1238
ApoI RAATTY 4 cut(s) 69, 144, 460, 542
Asp700I GAANNNNTTC 4 cut(s) 73, 315, 812, 1272
AspLEI GCGC 2 cut(s) 479, 496
AspS9I GGNCC 1 cut(s) 648
AsuC2I CCSGG 3 cut(s) 210, 211, 834
AsuHPI GGTGA 2 cut(s) 112, 155
AvaI CYCGRG 1 cut(s) 209
AvaII GGWCC 1 cut(s) 648
AxyI CCTNAGG 1 cut(s) 64
BaeGI GKGCMC 2 cut(s) 210, 1031
BalI TGGCCA 1 cut(s) 510
BanI GGYRCC 2 cut(s) 761, 1028
BanII GRGCYC 1 cut(s) 531
BbsI GAAGAC 1 cut(s) 1147
Bbv12I GWGCWC 3 cut(s) 386, 723, 1129
BbvI GCAGC 3 cut(s) 37, 1006, 1250
BccI CCATC 7 cut(s) 39, 131, 301, 430, 574, 665, 1031
BceAI ACGGC 2 cut(s) 713, 872
BclI TGATCA 1 cut(s) 990
BcnI CCSGG 3 cut(s) 210, 211, 834
BcoDI GTCTC 2 cut(s) 150, 1208
BcuI ACTAGT 3 cut(s) 820, 1100, 1111
BfaI CTAG 7 cut(s) 54, 93, 755, 821, 1101, 1112, 1378
BfoI RGCGCY 1 cut(s) 480
BfuAI ACCTGC 1 cut(s) 749
BisI GCNGC 4 cut(s) 51, 765, 1020, 1239
BlsI GCNGC 4 cut(s) 52, 766, 1021, 1240
Bme1390I CCNGG 3 cut(s) 210, 211, 834
Bme18I GGWCC 1 cut(s) 648
BmeT110I CYCGRG 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 648
BmiI GGNNCC 3 cut(s) 763, 1030, 1137
BmrFI CCNGG 3 cut(s) 210, 211, 834
BpiI GAAGAC 1 cut(s) 1147
BpmI CTGGAG 1 cut(s) 1113
BpuEI CTTGAG 2 cut(s) 1203, 1331
BpuMI CCSGG 3 cut(s) 210, 211, 834
Bsa29I ATCGAT 1 cut(s) 1347
BsaJI CCNNGG 5 cut(s) 151, 209, 519, 1023, 1032
BsaXI ACNNNNNCTCC 2 cut(s) 903, 933
Bsc4I CCNNNNNNNGG 6 cut(s) 230, 525, 570, 758, 833, 1085
Bse1I ACTGG 3 cut(s) 239, 379, 1130
Bse21I CCTNAGG 1 cut(s) 64
BseCI ATCGAT 1 cut(s) 1347
BseDI CCNNGG 5 cut(s) 151, 209, 519, 1023, 1032
BseGI GGATG 3 cut(s) 50, 220, 1042
BseLI CCNNNNNNNGG 6 cut(s) 230, 525, 570, 758, 833, 1085
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 3 cut(s) 239, 379, 1130
BseRI GAGGAG 1 cut(s) 1064
BseSI GKGCMC 2 cut(s) 210, 1031
BseXI GCAGC 3 cut(s) 37, 1006, 1250
BseYI CCCAGC 2 cut(s) 782, 828
BsgI GTGCAG 1 cut(s) 996
BshFI GGCC 3 cut(s) 432, 510, 887
BshNI GGYRCC 2 cut(s) 761, 1028
BshVI ATCGAT 1 cut(s) 1347
BsiHKAI GWGCWC 3 cut(s) 386, 723, 1129
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 3 cut(s) 210, 564, 834
BslFI GGGAC 1 cut(s) 809
BslI CCNNNNNNNGG 6 cut(s) 230, 525, 570, 758, 833, 1085
BsmAI GTCTC 2 cut(s) 150, 1208
BsmFI GGGAC 1 cut(s) 809
BsnI GGCC 3 cut(s) 432, 510, 887
BsoBI CYCGRG 1 cut(s) 209
Bsp1286I GDGCHC 6 cut(s) 210, 386, 531, 723, 1031, 1129
Bsp143I GATC 4 cut(s) 244, 256, 334, 990
Bsp19I CCATGG 2 cut(s) 151, 1032
BspACI CCGC 4 cut(s) 141, 483, 765, 770
BspANI GGCC 3 cut(s) 432, 510, 887
BspCNI CTCAG 1 cut(s) 77
BspDI ATCGAT 1 cut(s) 1347
BspHI TCATGA 1 cut(s) 997
BspLI GGNNCC 3 cut(s) 763, 1030, 1137
BspMI ACCTGC 1 cut(s) 749
BspPI GGATC 2 cut(s) 239, 264
BspQI GCTCTTC 1 cut(s) 711
BspT107I GGYRCC 2 cut(s) 761, 1028
BsrI ACTGG 3 cut(s) 239, 379, 1130
BssECI CCNNGG 5 cut(s) 151, 209, 519, 1023, 1032
BssMI GATC 4 cut(s) 244, 256, 334, 990
BssT1I CCWWGG 4 cut(s) 151, 519, 1023, 1032
Bst4CI ACNGT 4 cut(s) 205, 793, 925, 1054
Bst6I CTCTTC 1 cut(s) 711
BstAPI GCANNNNNTGC 1 cut(s) 35
BstC8I GCNNGC 3 cut(s) 36, 1091, 1189
BstDEI CTNAG 1 cut(s) 64
BstDSI CCRYGG 2 cut(s) 151, 1032
BstF5I GGATG 3 cut(s) 50, 220, 1042
BstH2I RGCGCY 1 cut(s) 480
BstHHI GCGC 2 cut(s) 479, 496
BstKTI GATC 4 cut(s) 247, 259, 337, 993
BstMAI GTCTC 2 cut(s) 150, 1208
BstMBI GATC 4 cut(s) 244, 256, 334, 990
BstMWI GCNNNNNNNGC 9 cut(s) 35, 147, 290, 491, 606, 764, 911, 1144, 1193
BstNSI RCATGY 1 cut(s) 38
BstSCI CCNGG 3 cut(s) 208, 209, 832
BstSLI GKGCMC 2 cut(s) 210, 1031
BstV1I GCAGC 3 cut(s) 37, 1006, 1250
BstV2I GAAGAC 1 cut(s) 1147
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
Bsu15I ATCGAT 1 cut(s) 1347
Bsu36I CCTNAGG 1 cut(s) 64
BsuRI GGCC 3 cut(s) 432, 510, 887
BsuTUI ATCGAT 1 cut(s) 1347
BtgI CCRYGG 2 cut(s) 151, 1032
BtgZI GCGATG 1 cut(s) 320
BtsCI GGATG 3 cut(s) 50, 220, 1042
BtsI GCAGTG 4 cut(s) 36, 966, 1198, 1293
BtsIMutI CAGTG 9 cut(s) 36, 386, 661, 921, 966, 1050, 1137, 1198, 1293
BveI ACCTGC 1 cut(s) 749
Cac8I GCNNGC 3 cut(s) 36, 1091, 1189
CciI TCATGA 1 cut(s) 997
CfoI GCGC 2 cut(s) 479, 496
Cfr13I GGNCC 1 cut(s) 648
Cfr9I CCCGGG 1 cut(s) 209
ClaI ATCGAT 1 cut(s) 1347
CviAII CATG 9 cut(s) 35, 152, 497, 577, 588, 597, 998, 1004, 1033
DdeI CTNAG 1 cut(s) 64
DpnI GATC 4 cut(s) 246, 258, 336, 992
DpnII GATC 4 cut(s) 244, 256, 334, 990
EaeI YGGCCR 2 cut(s) 508, 885
Eam1104I CTCTTC 1 cut(s) 711
EarI CTCTTC 1 cut(s) 711
Eco130I CCWWGG 4 cut(s) 151, 519, 1023, 1032
Eco24I GRGCYC 1 cut(s) 531
Eco32I GATATC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 648
Eco57I CTGAAG 1 cut(s) 637
Eco81I CCTNAGG 1 cut(s) 64
Eco88I CYCGRG 1 cut(s) 209
EcoRI GAATTC 1 cut(s) 69
EcoRV GATATC 1 cut(s) 118
EcoT14I CCWWGG 4 cut(s) 151, 519, 1023, 1032
EcoT38I GRGCYC 1 cut(s) 531
ErhI CCWWGG 4 cut(s) 151, 519, 1023, 1032
FaeI CATG 9 cut(s) 38, 155, 500, 580, 591, 600, 1001, 1007, 1036
FaqI GGGAC 1 cut(s) 809
FatI CATG 9 cut(s) 34, 151, 496, 576, 587, 596, 997, 1003, 1032
FauI CCCGC 1 cut(s) 777
FauNDI CATATG 1 cut(s) 882
FbaI TGATCA 1 cut(s) 990
FblI GTMKAC 1 cut(s) 188
Fnu4HI GCNGC 4 cut(s) 51, 765, 1020, 1239
FokI GGATG 3 cut(s) 57, 207, 1049
FriOI GRGCYC 1 cut(s) 531
Fsp4HI GCNGC 4 cut(s) 51, 765, 1020, 1239
FspBI CTAG 7 cut(s) 54, 93, 755, 821, 1101, 1112, 1378
GlaI GCGC 2 cut(s) 478, 495
GluI GCNGC 4 cut(s) 51, 765, 1020, 1239
GsaI CCCAGC 2 cut(s) 786, 832
GsuI CTGGAG 1 cut(s) 1113
HaeII RGCGCY 1 cut(s) 480
HaeIII GGCC 3 cut(s) 432, 510, 887
HapII CCGG 3 cut(s) 210, 564, 834
HhaI GCGC 2 cut(s) 479, 496
Hin1II CATG 9 cut(s) 38, 155, 500, 580, 591, 600, 1001, 1007, 1036
Hin6I GCGC 2 cut(s) 477, 494
HinP1I GCGC 2 cut(s) 477, 494
HincII GTYRAC 1 cut(s) 189
HindII GTYRAC 1 cut(s) 189
HindIII AAGCTT 1 cut(s) 903
HinfI GANTC 6 cut(s) 59, 366, 425, 735, 980, 1216
HpaII CCGG 3 cut(s) 210, 564, 834
HphI GGTGA 2 cut(s) 112, 155
Hpy166II GTNNAC 4 cut(s) 189, 264, 659, 1291
Hpy188I TCNGA 8 cut(s) 201, 329, 339, 700, 734, 818, 911, 942
Hpy188III TCNNGA 8 cut(s) 20, 66, 127, 363, 370, 998, 1220, 1301
Hpy8I GTNNAC 4 cut(s) 189, 264, 659, 1291
HpyAV CCTTC 4 cut(s) 90, 234, 661, 1309
HpyCH4III ACNGT 4 cut(s) 205, 793, 925, 1054
HpyCH4IV ACGT 1 cut(s) 534
HpyCH4V TGCA 7 cut(s) 34, 576, 609, 977, 1196, 1238, 1373
HpyF10VI GCNNNNNNNGC 9 cut(s) 35, 147, 290, 491, 606, 764, 911, 1144, 1193
HpyF3I CTNAG 1 cut(s) 64
HpySE526I ACGT 1 cut(s) 534
Hsp92II CATG 9 cut(s) 38, 155, 500, 580, 591, 600, 1001, 1007, 1036
HspAI GCGC 2 cut(s) 477, 494
Ksp22I TGATCA 1 cut(s) 990
Kzo9I GATC 4 cut(s) 244, 256, 334, 990
LguI GCTCTTC 1 cut(s) 711
LmnI GCTCC 3 cut(s) 911, 1132, 1135
Lsp1109I GCAGC 3 cut(s) 37, 1006, 1250
MaeI CTAG 7 cut(s) 54, 93, 755, 821, 1101, 1112, 1378
MaeII ACGT 1 cut(s) 534
MaeIII GTNAC 3 cut(s) 487, 514, 919
MalI GATC 4 cut(s) 246, 258, 336, 992
MboI GATC 4 cut(s) 244, 256, 334, 990
MboII GAAGA 6 cut(s) 35, 323, 436, 680, 728, 1147
MfeI CAATTG 2 cut(s) 1010, 1197
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 6 cut(s) 210, 386, 531, 723, 1031, 1129
MlsI TGGCCA 1 cut(s) 510
MluCI AATT 9 cut(s) 69, 144, 460, 501, 542, 625, 1010, 1164, 1197
MluNI TGGCCA 1 cut(s) 510
MlyI GAGTC 2 cut(s) 419, 1210
MmeI TCCRAC 2 cut(s) 224, 532
MnlI CCTC 7 cut(s) 73, 176, 863, 946, 1009, 1085, 1167
Mox20I TGGCCA 1 cut(s) 510
MroXI GAANNNNTTC 4 cut(s) 73, 315, 812, 1272
MscI TGGCCA 1 cut(s) 510
MseI TTAA 2 cut(s) 1163, 1329
MslI CAYNNNNRTG 3 cut(s) 39, 852, 1002
Msp20I TGGCCA 1 cut(s) 510
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 3 cut(s) 210, 564, 834
MspR9I CCNGG 3 cut(s) 210, 211, 834
MunI CAATTG 2 cut(s) 1010, 1197
MwoI GCNNNNNNNGC 9 cut(s) 35, 147, 290, 491, 606, 764, 911, 1144, 1193
NciI CCSGG 3 cut(s) 210, 211, 834
NcoI CCATGG 2 cut(s) 151, 1032
NdeI CATATG 1 cut(s) 882
NdeII GATC 4 cut(s) 244, 256, 334, 990
NlaIII CATG 9 cut(s) 38, 155, 500, 580, 591, 600, 1001, 1007, 1036
NlaIV GGNNCC 3 cut(s) 763, 1030, 1137
NmuCI GTSAC 3 cut(s) 487, 514, 919
NspI RCATGY 1 cut(s) 38
PaeI GCATGC 1 cut(s) 38
PagI TCATGA 1 cut(s) 997
PaqCI CACCTGC 1 cut(s) 749
PciSI GCTCTTC 1 cut(s) 711
PdmI GAANNNNTTC 4 cut(s) 73, 315, 812, 1272
PfeI GAWTC 4 cut(s) 59, 366, 735, 980
PflMI CCANNNNNTGG 1 cut(s) 1085
PkrI GCNGC 4 cut(s) 52, 766, 1021, 1240
PleI GAGTC 2 cut(s) 419, 1210
PpsI GAGTC 2 cut(s) 419, 1210
PspFI CCCAGC 2 cut(s) 782, 828
PspN4I GGNNCC 3 cut(s) 763, 1030, 1137
PspPI GGNCC 1 cut(s) 648
PsuI RGATCY 1 cut(s) 256
RseI CAYNNNNRTG 3 cut(s) 39, 852, 1002
SalI GTCGAC 1 cut(s) 187
SapI GCTCTTC 1 cut(s) 711
SaqAI TTAA 2 cut(s) 1163, 1329
SatI GCNGC 4 cut(s) 51, 765, 1020, 1239
Sau3AI GATC 4 cut(s) 244, 256, 334, 990
Sau96I GGNCC 1 cut(s) 648
SchI GAGTC 2 cut(s) 419, 1210
ScrFI CCNGG 3 cut(s) 210, 211, 834
SduI GDGCHC 6 cut(s) 210, 386, 531, 723, 1031, 1129
SinI GGWCC 1 cut(s) 648
SmaI CCCGGG 1 cut(s) 211
SmiMI CAYNNNNRTG 3 cut(s) 39, 852, 1002
SmlI CTYRAG 3 cut(s) 18, 1218, 1310
SmoI CTYRAG 3 cut(s) 18, 1218, 1310
SpeI ACTAGT 3 cut(s) 820, 1100, 1111
SphI GCATGC 1 cut(s) 38
Sse9I AATT 9 cut(s) 69, 144, 460, 501, 542, 625, 1010, 1164, 1197
SsiI CCGC 4 cut(s) 141, 483, 765, 770
SspI AATATT 2 cut(s) 137, 812
SspMI CTAG 7 cut(s) 54, 93, 755, 821, 1101, 1112, 1378
StyD4I CCNGG 3 cut(s) 208, 209, 832
StyI CCWWGG 4 cut(s) 151, 519, 1023, 1032
TaaI ACNGT 4 cut(s) 205, 793, 925, 1054
TaiI ACGT 1 cut(s) 537
TaqI TCGA 5 cut(s) 188, 423, 1047, 1266, 1347
TasI AATT 9 cut(s) 69, 144, 460, 501, 542, 625, 1010, 1164, 1197
TauI GCSGC 1 cut(s) 767
TfiI GAWTC 4 cut(s) 59, 366, 735, 980
Tru1I TTAA 2 cut(s) 1163, 1329
Tru9I TTAA 2 cut(s) 1163, 1329
TscAI CASTG 9 cut(s) 36, 386, 661, 928, 966, 1057, 1137, 1198, 1300
TseFI GTSAC 3 cut(s) 487, 514, 919
TseI GCWGC 3 cut(s) 50, 1019, 1238
Tsp45I GTSAC 3 cut(s) 487, 514, 919
TspDTI ATGAA 7 cut(s) 209, 436, 513, 576, 681, 972, 1223
TspGWI ACGGA 1 cut(s) 311
TspMI CCCGGG 1 cut(s) 209
TspRI CASTG 9 cut(s) 36, 386, 661, 928, 966, 1057, 1137, 1198, 1300
Van91I CCANNNNNTGG 1 cut(s) 1085
VpaK11BI GGWCC 1 cut(s) 648
XapI RAATTY 4 cut(s) 69, 144, 460, 542
XceI RCATGY 1 cut(s) 38
XmaI CCCGGG 1 cut(s) 209
XmiI GTMKAC 1 cut(s) 188
XmnI GAANNNNTTC 4 cut(s) 73, 315, 812, 1272
XspI CTAG 7 cut(s) 54, 93, 755, 821, 1101, 1112, 1378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.