Rmu_sc0000389.1_g000036

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000389.1
OG0002881
Physical Location & Seq
Forward (+)
158898 .. 161530
2633 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 837 bp
atgattttgtatagacatgagatcgtcggagaagtgaccgaagtcggaagcaacgtcacgaaattcaaagtaggagacatagccggagtggggtgcatggtggtttcatgtggttcgtgcaataaccgcacacgagacttggaaagttactgccccaatatgatccataacaaacactaccacgacggatccagaacttttgggggatactccgacaaactagtagtcgaggagcatttcgcagttcgaattccagataacttgtcattggaaggtgttgcacctttgctatgtactgggattacggtgtacagccctacgaaacactttggactttttaagcatgattacctcaatctccatcgtcatcgttctgggtctgattgggagggatattgcaaatccggtcagaagggaatcgagttgggttttggggaagaagatgatgggggatgtggaactttgcaagttgcgagttgtgtggcaatcaaacaaaaagaagaagatgatgatggaactgataaagaagaagacgccgagctgaagctgacttccggtacggctcagccgagccggaaagtcggtaagccgaatcttcggcccaaatcggttcggaccggctcagatgtaggaaggaagttaattgctggtagtgcaattgggggaatgaaagagactcaagagatggtagattttgcagcaaagcataacatcacagctgatgttgaagtcattccaatggattatgtgaatactgctcttaaaagagttagtaaggctgatgttaactaccgctttgtcattgatgttacaaacaccttgaattctccatattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

278

Amino Acids

30.45

Weight (kDa)

5.94

Isoelectric Point (pI)

32.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 6 - 86 1.7e-11 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 127, 793
AclWI GGATC 3 cut(s) 157, 183, 196
AcsI RAATTY 3 cut(s) 62, 249, 823
AcuI CTGAAG 1 cut(s) 563
AcyI GRCGYC 1 cut(s) 534
AfaI GTAC 3 cut(s) 295, 311, 559
AfiI CCNNNNNNNGG 1 cut(s) 90
AgsI TTSAA 3 cut(s) 67, 728, 823
AhlI ACTAGT 1 cut(s) 220
AluBI AGCT 3 cut(s) 541, 547, 719
AluI AGCT 3 cut(s) 541, 547, 719
Alw26I GTCTC 3 cut(s) 69, 129, 668
AlwI GGATC 3 cut(s) 157, 183, 196
AoxI GGCC 1 cut(s) 599
ApeKI GCWGC 1 cut(s) 698
ApoI RAATTY 3 cut(s) 62, 249, 823
ArsI GACNNNNNNTTYG 2 cut(s) 210, 242
Asp700I GAANNNNTTC 1 cut(s) 732
AspS9I GGNCC 2 cut(s) 600, 615
AsuII TTCGAA 1 cut(s) 247
AvaII GGWCC 1 cut(s) 615
BamHI GGATCC 1 cut(s) 188
BauI CACGAG 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 537
BbvI GCAGC 1 cut(s) 710
BccI CCATC 4 cut(s) 369, 440, 506, 679
BceAI ACGGC 1 cut(s) 576
BciVI GTATCC 1 cut(s) 200
BcoDI GTCTC 3 cut(s) 69, 129, 668
BcuI ACTAGT 1 cut(s) 220
BfaI CTAG 1 cut(s) 221
BfuI GTATCC 1 cut(s) 200
BisI GCNGC 1 cut(s) 699
BlpI GCTNAGC 1 cut(s) 564
BlsI GCNGC 1 cut(s) 700
Bme18I GGWCC 1 cut(s) 615
BmgT120I GGNCC 2 cut(s) 600, 615
BmiI GGNNCC 1 cut(s) 190
BmrI ACTGGG 1 cut(s) 306
BmuI ACTGGG 1 cut(s) 306
BoxI GACNNNNGTC 1 cut(s) 41
BpiI GAAGAC 1 cut(s) 537
Bpu1102I GCTNAGC 1 cut(s) 564
Bpu14I TTCGAA 1 cut(s) 247
BpuEI CTTGAG 1 cut(s) 663
BsaHI GRCGYC 1 cut(s) 534
BsaWI WCCGGW 2 cut(s) 404, 554
Bsc4I CCNNNNNNNGG 1 cut(s) 90
Bse118I RCCGGY 1 cut(s) 617
Bse1I ACTGG 1 cut(s) 301
BseGI GGATG 1 cut(s) 458
BseLI CCNNNNNNNGG 1 cut(s) 90
BseMII CTCAG 2 cut(s) 578, 636
BseNI ACTGG 1 cut(s) 301
BseRI GAGGAG 1 cut(s) 245
BseXI GCAGC 1 cut(s) 710
BshFI GGCC 1 cut(s) 601
BsiSI CCGG 5 cut(s) 84, 405, 555, 574, 618
BslI CCNNNNNNNGG 1 cut(s) 90
BsmAI GTCTC 3 cut(s) 69, 129, 668
BsnI GGCC 1 cut(s) 601
Bsp119I TTCGAA 1 cut(s) 247
Bsp1407I TGTACA 1 cut(s) 309
Bsp143I GATC 3 cut(s) 21, 162, 188
Bsp1720I GCTNAGC 1 cut(s) 564
BspACI CCGC 2 cut(s) 127, 793
BspANI GGCC 1 cut(s) 601
BspCNI CTCAG 2 cut(s) 577, 635
BspLI GGNNCC 1 cut(s) 190
BspPI GGATC 3 cut(s) 157, 183, 196
BspT104I TTCGAA 1 cut(s) 247
BsrFI RCCGGY 1 cut(s) 617
BsrGI TGTACA 1 cut(s) 309
BsrI ACTGG 1 cut(s) 301
BssAI RCCGGY 1 cut(s) 617
BssMI GATC 3 cut(s) 21, 162, 188
BssNI GRCGYC 1 cut(s) 534
BssSI CACGAG 1 cut(s) 132
Bst2BI CACGAG 1 cut(s) 132
Bst4CI ACNGT 1 cut(s) 307
BstACI GRCGYC 1 cut(s) 534
BstAUI TGTACA 1 cut(s) 309
BstBI TTCGAA 1 cut(s) 247
BstDEI CTNAG 2 cut(s) 564, 622
BstF5I GGATG 1 cut(s) 458
BstKTI GATC 3 cut(s) 24, 165, 191
BstMAI GTCTC 3 cut(s) 69, 129, 668
BstMBI GATC 3 cut(s) 21, 162, 188
BstMWI GCNNNNNNNGC 2 cut(s) 126, 653
BstPAI GACNNNNGTC 1 cut(s) 41
BstV1I GCAGC 1 cut(s) 710
BstV2I GAAGAC 1 cut(s) 537
BstX2I RGATCY 1 cut(s) 188
BstYI RGATCY 1 cut(s) 188
BsuI GTATCC 1 cut(s) 200
BsuRI GGCC 1 cut(s) 601
BtsCI GGATG 1 cut(s) 458
Cfr10I RCCGGY 1 cut(s) 617
Cfr13I GGNCC 2 cut(s) 600, 615
CpoI CGGWCCG 1 cut(s) 615
CseI GACGC 1 cut(s) 542
Csp6I GTAC 3 cut(s) 294, 310, 558
CspI CGGWCCG 1 cut(s) 615
CviAII CATG 4 cut(s) 17, 97, 108, 344
CviQI GTAC 3 cut(s) 294, 310, 558
DdeI CTNAG 2 cut(s) 564, 622
DpnI GATC 3 cut(s) 23, 164, 190
DpnII GATC 3 cut(s) 21, 162, 188
Eco47I GGWCC 1 cut(s) 615
Eco57I CTGAAG 1 cut(s) 563
EcoRI GAATTC 2 cut(s) 249, 823
FaeI CATG 4 cut(s) 20, 100, 111, 347
FatI CATG 4 cut(s) 16, 96, 107, 343
Fnu4HI GCNGC 1 cut(s) 699
FokI GGATG 1 cut(s) 465
Fsp4HI GCNGC 1 cut(s) 699
FspBI CTAG 1 cut(s) 221
GluI GCNGC 1 cut(s) 699
HaeIII GGCC 1 cut(s) 601
HapII CCGG 5 cut(s) 84, 405, 555, 574, 618
HgaI GACGC 1 cut(s) 542
Hin1I GRCGYC 1 cut(s) 534
Hin1II CATG 4 cut(s) 20, 100, 111, 347
HincII GTYRAC 1 cut(s) 787
HindII GTYRAC 1 cut(s) 787
HinfI GANTC 3 cut(s) 417, 592, 676
HpaI GTTAAC 1 cut(s) 787
HpaII CCGG 5 cut(s) 84, 405, 555, 574, 618
Hpy166II GTNNAC 2 cut(s) 310, 787
Hpy188I TCNGA 7 cut(s) 29, 47, 214, 382, 411, 615, 625
Hpy188III TCNNGA 4 cut(s) 58, 192, 254, 680
Hpy8I GTNNAC 2 cut(s) 310, 787
Hpy99I CGWCG 2 cut(s) 29, 188
HpyAV CCTTC 3 cut(s) 266, 406, 627
HpyCH4III ACNGT 1 cut(s) 307
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 7 cut(s) 96, 120, 281, 399, 466, 656, 698
HpyF10VI GCNNNNNNNGC 2 cut(s) 126, 653
HpyF3I CTNAG 2 cut(s) 564, 622
HpySE526I ACGT 1 cut(s) 54
Hsp92I GRCGYC 1 cut(s) 534
Hsp92II CATG 4 cut(s) 20, 100, 111, 347
KspAI GTTAAC 1 cut(s) 787
Kzo9I GATC 3 cut(s) 21, 162, 188
LmnI GCTCC 1 cut(s) 232
Lsp1109I GCAGC 1 cut(s) 710
MaeI CTAG 1 cut(s) 221
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 4 cut(s) 34, 55, 146, 808
MalI GATC 3 cut(s) 23, 164, 190
MboI GATC 3 cut(s) 21, 162, 188
MboII GAAGA 7 cut(s) 449, 452, 512, 515, 539, 542, 587
MfeI CAATTG 1 cut(s) 657
MflI RGATCY 1 cut(s) 188
MluCI AATT 5 cut(s) 62, 249, 642, 657, 823
MlyI GAGTC 1 cut(s) 670
MmeI TCCRAC 3 cut(s) 7, 25, 237
MnlI CCTC 3 cut(s) 223, 362, 382
MroXI GAANNNNTTC 1 cut(s) 732
MseI TTAA 5 cut(s) 339, 641, 762, 786, 835
MslI CAYNNNNRTG 1 cut(s) 737
MspA1I CMGCKG 1 cut(s) 719
MspI CCGG 5 cut(s) 84, 405, 555, 574, 618
MunI CAATTG 1 cut(s) 657
MwoI GCNNNNNNNGC 2 cut(s) 126, 653
NdeII GATC 3 cut(s) 21, 162, 188
NlaIII CATG 4 cut(s) 20, 100, 111, 347
NlaIV GGNNCC 1 cut(s) 190
NmeAIII GCCGAG 2 cut(s) 562, 594
NmuCI GTSAC 2 cut(s) 34, 55
NspV TTCGAA 1 cut(s) 247
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 566
PdmI GAANNNNTTC 1 cut(s) 732
PfeI GAWTC 2 cut(s) 417, 592
PkrI GCNGC 1 cut(s) 700
PleI GAGTC 1 cut(s) 670
PpsI GAGTC 1 cut(s) 670
PshAI GACNNNNGTC 1 cut(s) 41
PspN4I GGNNCC 1 cut(s) 190
PspPI GGNCC 2 cut(s) 600, 615
PsuI RGATCY 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 719
RsaI GTAC 3 cut(s) 295, 311, 559
RsaNI GTAC 3 cut(s) 294, 310, 558
RseI CAYNNNNRTG 1 cut(s) 737
Rsr2I CGGWCCG 1 cut(s) 615
RsrII CGGWCCG 1 cut(s) 615
SaqAI TTAA 5 cut(s) 339, 641, 762, 786, 835
SatI GCNGC 1 cut(s) 699
Sau3AI GATC 3 cut(s) 21, 162, 188
Sau96I GGNCC 2 cut(s) 600, 615
SchI GAGTC 1 cut(s) 670
SetI ASST 8 cut(s) 57, 277, 286, 354, 543, 549, 721, 821
SfuI TTCGAA 1 cut(s) 247
SinI GGWCC 1 cut(s) 615
SmiMI CAYNNNNRTG 1 cut(s) 737
SmlI CTYRAG 1 cut(s) 678
SmoI CTYRAG 1 cut(s) 678
SpeI ACTAGT 1 cut(s) 220
Sse9I AATT 5 cut(s) 62, 249, 642, 657, 823
SsiI CCGC 2 cut(s) 127, 793
SspMI CTAG 1 cut(s) 221
TaaI ACNGT 1 cut(s) 307
TaiI ACGT 1 cut(s) 57
TaqI TCGA 3 cut(s) 228, 247, 420
TaqII GACCGA 1 cut(s) 53
TasI AATT 5 cut(s) 62, 249, 642, 657, 823
TatI WGTACW 2 cut(s) 293, 309
TfiI GAWTC 2 cut(s) 417, 592
Tru1I TTAA 5 cut(s) 339, 641, 762, 786, 835
Tru9I TTAA 5 cut(s) 339, 641, 762, 786, 835
TseFI GTSAC 2 cut(s) 34, 55
TseI GCWGC 1 cut(s) 698
Tsp45I GTSAC 2 cut(s) 34, 55
TspDTI ATGAA 2 cut(s) 96, 683
TspGWI ACGGA 1 cut(s) 201
VpaK11BI GGWCC 1 cut(s) 615
XapI RAATTY 3 cut(s) 62, 249, 823
XmnI GAANNNNTTC 1 cut(s) 732
XspI CTAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.