Rmu_sc0000554.1_g000027

zinc ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000554.1
OG0002517
Physical Location & Seq
Reverse (-)
136914 .. 137351
438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000554.1_g000027.1.cds

Sequence Viewer

Length: 438 bp
atggactgtccttcaccactgtgtgtcaaaggttgtggtttctttgggacttcggcaaacaagaatatgtgctcaaagtgctacacagagtatctgaaagaacagcttcttgctaagcctactgcagcgccggccgtggtggcttcagtgaaaaaaaccctagacgtcactgtttctgctagcgagtcgttgtcaactgctacaactactgatcagccatgtggtgcgacaaagaataagtgccagaacgagagctgcaacaagaaacttaacctggtggaaatgatgacgttaaaatgtcgctgtggtggaatgttttgtacgaaggataagtatccttaccactcatgtagtgtgaattacaagaaacctggacgagagcttttggccaagcaaaacccaatttgccaggctgacaagttaaagtatagggtttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-A20 zf-AN1

Protein Analysis

145

Amino Acids

15.87

Weight (kDa)

9.1

Isoelectric Point (pI)

29.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 168
AcoI YGGCCR 2 cut(s) 132, 387
AcuI CTGAAG 1 cut(s) 129
AcyI GRCGYC 1 cut(s) 165
AdeI CACNNNGTG 1 cut(s) 23
AfaI GTAC 1 cut(s) 322
AjnI CCWGG 3 cut(s) 273, 370, 408
AleI CACNNNNGTG 1 cut(s) 19
AluBI AGCT 3 cut(s) 106, 255, 382
AluI AGCT 3 cut(s) 106, 255, 382
Alw21I GWGCWC 1 cut(s) 74
AoxI GGCC 2 cut(s) 132, 387
ApeKI GCWGC 2 cut(s) 125, 255
Asp700I GAANNNNTTC 1 cut(s) 105
AspLEI GCGC 1 cut(s) 130
AsuHPI GGTGA 1 cut(s) 6
AsuNHI GCTAGC 1 cut(s) 179
BalI TGGCCA 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 74
BbvI GCAGC 2 cut(s) 137, 242
BceAI ACGGC 1 cut(s) 119
BciT130I CCWGG 3 cut(s) 275, 372, 410
BciVI GTATCC 1 cut(s) 345
BclI TGATCA 1 cut(s) 211
BfaI CTAG 2 cut(s) 161, 180
BfmI CTRYAG 1 cut(s) 123
BfoI RGCGCY 1 cut(s) 131
BfuI GTATCC 1 cut(s) 345
BglI GCCNNNNNGGC 1 cut(s) 140
BisI GCNGC 2 cut(s) 126, 256
BlpI GCTNAGC 1 cut(s) 114
BlsI GCNGC 2 cut(s) 127, 257
Bme1390I CCNGG 3 cut(s) 275, 372, 410
BmrFI CCNGG 3 cut(s) 275, 372, 410
BmtI GCTAGC 1 cut(s) 183
Bpu1102I GCTNAGC 1 cut(s) 114
BsaBI GATNNNNATC 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 135
Bse118I RCCGGY 1 cut(s) 130
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 3 cut(s) 275, 372, 410
BseDI CCNNGG 1 cut(s) 135
BseJI GATNNNNATC 1 cut(s) 333
BseX3I CGGCCG 1 cut(s) 132
BseXI GCAGC 2 cut(s) 137, 242
Bsh1285I CGRYCG 1 cut(s) 135
BshFI GGCC 2 cut(s) 134, 389
BsiEI CGRYCG 1 cut(s) 135
BsiHKAI GWGCWC 1 cut(s) 74
BsiSI CCGG 1 cut(s) 131
BslFI GGGAC 1 cut(s) 61
BsmFI GGGAC 1 cut(s) 61
BsnI GGCC 2 cut(s) 134, 389
Bsp1286I GDGCHC 1 cut(s) 74
Bsp143I GATC 1 cut(s) 211
Bsp1720I GCTNAGC 1 cut(s) 114
BspANI GGCC 2 cut(s) 134, 389
BspMAI CTGCAG 1 cut(s) 127
BspOI GCTAGC 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 130
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 1 cut(s) 135
BssMI GATC 1 cut(s) 211
BssNI GRCGYC 1 cut(s) 165
Bst2UI CCWGG 3 cut(s) 275, 372, 410
Bst4CI ACNGT 3 cut(s) 8, 21, 172
BstACI GRCGYC 1 cut(s) 165
BstC8I GCNNGC 2 cut(s) 132, 181
BstDEI CTNAG 1 cut(s) 114
BstDSI CCRYGG 1 cut(s) 135
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 1 cut(s) 214
BstMBI GATC 1 cut(s) 211
BstMCI CGRYCG 1 cut(s) 135
BstMWI GCNNNNNNNGC 3 cut(s) 78, 131, 140
BstNI CCWGG 3 cut(s) 275, 372, 410
BstSCI CCNGG 3 cut(s) 273, 370, 408
BstSFI CTRYAG 1 cut(s) 123
BstV1I GCAGC 2 cut(s) 137, 242
BstZI CGGCCG 1 cut(s) 132
BsuI GTATCC 1 cut(s) 345
BsuRI GGCC 2 cut(s) 134, 389
BtgI CCRYGG 1 cut(s) 135
BtsIMutI CAGTG 3 cut(s) 17, 153, 168
Cac8I GCNNGC 2 cut(s) 132, 181
CfoI GCGC 1 cut(s) 130
Cfr10I RCCGGY 1 cut(s) 130
CsiI ACCWGGT 1 cut(s) 273
Csp6I GTAC 1 cut(s) 321
CspCI CAANNNNNGTGG 2 cut(s) 16, 51
CviAII CATG 2 cut(s) 219, 348
CviJI RGCY 9 cut(s) 106, 118, 134, 143, 217, 255, 382, 389, 413
CviKI_1 RGCY 9 cut(s) 106, 118, 134, 143, 217, 255, 382, 389, 413
CviQI GTAC 1 cut(s) 321
DdeI CTNAG 1 cut(s) 114
DpnI GATC 1 cut(s) 213
DpnII GATC 1 cut(s) 211
DraIII CACNNNGTG 1 cut(s) 23
EaeI YGGCCR 2 cut(s) 132, 387
EagI CGGCCG 1 cut(s) 132
EclXI CGGCCG 1 cut(s) 132
Eco52I CGGCCG 1 cut(s) 132
Eco57I CTGAAG 1 cut(s) 129
EcoRII CCWGG 3 cut(s) 273, 370, 408
FaeI CATG 2 cut(s) 222, 351
FaiI YATR 4 cut(s) 68, 220, 349, 429
FalI AAGNNNNNCTT 2 cut(s) 90, 122
FaqI GGGAC 1 cut(s) 61
FatI CATG 2 cut(s) 218, 347
FbaI TGATCA 1 cut(s) 211
Fnu4HI GCNGC 2 cut(s) 126, 256
Fsp4HI GCNGC 2 cut(s) 126, 256
FspBI CTAG 2 cut(s) 161, 180
GlaI GCGC 1 cut(s) 129
GluI GCNGC 2 cut(s) 126, 256
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 2 cut(s) 134, 389
HapII CCGG 1 cut(s) 131
HhaI GCGC 1 cut(s) 130
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 2 cut(s) 222, 351
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HincII GTYRAC 1 cut(s) 195
HindII GTYRAC 1 cut(s) 195
HinfI GANTC 1 cut(s) 185
HpaII CCGG 1 cut(s) 131
HphI GGTGA 1 cut(s) 6
Hpy166II GTNNAC 1 cut(s) 195
Hpy188I TCNGA 1 cut(s) 96
Hpy8I GTNNAC 1 cut(s) 195
HpyAV CCTTC 2 cut(s) 21, 319
HpyCH4III ACNGT 3 cut(s) 8, 21, 172
HpyCH4IV ACGT 2 cut(s) 165, 290
HpyCH4V TGCA 2 cut(s) 125, 258
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 131, 140
HpyF3I CTNAG 1 cut(s) 114
HpySE526I ACGT 2 cut(s) 165, 290
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 2 cut(s) 222, 351
HspAI GCGC 1 cut(s) 128
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 1 cut(s) 211
LpnPI CCDG 8 cut(s) 144, 257, 260, 287, 357, 384, 395, 422
Lsp1109I GCAGC 2 cut(s) 137, 242
MabI ACCWGGT 1 cut(s) 273
MaeI CTAG 2 cut(s) 161, 180
MaeII ACGT 2 cut(s) 165, 290
MaeIII GTNAC 1 cut(s) 166
MalI GATC 1 cut(s) 213
MboI GATC 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 74
MlsI TGGCCA 1 cut(s) 389
MluCI AATT 2 cut(s) 358, 402
MluNI TGGCCA 1 cut(s) 389
MlyI GAGTC 1 cut(s) 194
Mox20I TGGCCA 1 cut(s) 389
MroNI GCCGGC 1 cut(s) 130
MroXI GAANNNNTTC 1 cut(s) 105
MscI TGGCCA 1 cut(s) 389
MseI TTAA 3 cut(s) 270, 293, 422
MslI CAYNNNNRTG 1 cut(s) 19
Msp20I TGGCCA 1 cut(s) 389
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 3 cut(s) 275, 372, 410
MvaI CCWGG 3 cut(s) 275, 372, 410
MwoI GCNNNNNNNGC 3 cut(s) 78, 131, 140
NaeI GCCGGC 1 cut(s) 132
NdeII GATC 1 cut(s) 211
NgoMIV GCCGGC 1 cut(s) 130
NheI GCTAGC 1 cut(s) 179
NlaIII CATG 2 cut(s) 222, 351
NmuCI GTSAC 1 cut(s) 166
OliI CACNNNNGTG 1 cut(s) 19
PdiI GCCGGC 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 105
PkrI GCNGC 2 cut(s) 127, 257
PleI GAGTC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 193
Psp6I CCWGG 3 cut(s) 273, 370, 408
PspGI CCWGG 3 cut(s) 273, 370, 408
PstI CTGCAG 1 cut(s) 127
RsaI GTAC 1 cut(s) 322
RsaNI GTAC 1 cut(s) 321
RseI CAYNNNNRTG 1 cut(s) 19
SaqAI TTAA 3 cut(s) 270, 293, 422
SatI GCNGC 2 cut(s) 126, 256
Sau3AI GATC 1 cut(s) 211
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 3 cut(s) 275, 372, 410
SduI GDGCHC 1 cut(s) 74
SetI ASST 8 cut(s) 34, 108, 168, 257, 276, 293, 373, 384
SexAI ACCWGGT 1 cut(s) 273
SfcI CTRYAG 1 cut(s) 123
SmiMI CAYNNNNRTG 1 cut(s) 19
Sse9I AATT 2 cut(s) 358, 402
SspMI CTAG 2 cut(s) 161, 180
StyD4I CCNGG 3 cut(s) 273, 370, 408
TaaI ACNGT 3 cut(s) 8, 21, 172
TaiI ACGT 2 cut(s) 168, 293
TasI AATT 2 cut(s) 358, 402
Tru1I TTAA 3 cut(s) 270, 293, 422
Tru9I TTAA 3 cut(s) 270, 293, 422
TscAI CASTG 3 cut(s) 24, 153, 175
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 2 cut(s) 125, 255
Tsp45I GTSAC 1 cut(s) 166
TspRI CASTG 3 cut(s) 24, 153, 175
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 2 cut(s) 161, 180
ZraI GACGTC 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.