Rh3DG101100

zinc ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0002517
Physical Location & Seq
Forward (+)
8066584 .. 8067283
700 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG101100.1

Sequence Viewer

Length: 444 bp
ATGGAGTCTCCAAGCAACATGGACTGTCCCCCTCCACTCTGCGCAAATGGTTGCGGTTTCTTTGGGACTTCGGCAAACAAGAATATGTGCTCAAAGTGCTACACGGAGTATCTGAAAGAAAAGCTTCTTGCTAAGCCTACTGCAGCGCGGGCTGTGGTGGTGGCTTCAGTGGAGAAAACCCTAGACGTCGCTGTTTCTGCTAGCGAGTCGTCTTCAAGTACTGCAACTAATGATCAGCGTCCTTGTGGTGTGACAAAGAATAGGTGCCAGAGCTGCGATTGCAACAAGAAACTTAAGCCGGCTCAAATGACATTAAAATGCCACTGTGGTGGAGTGTTTTGTGCAGAGCATAGGTTGGCTGAATCCCACACATGTAGTGTGAATTACAAGAAGGCTGGACAAGAGCTTTTGACCAAGCAAAACCCAGTTTGCAAGGGTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-A20 zf-AN1

Protein Analysis

147

Amino Acids

15.73

Weight (kDa)

8.64

Isoelectric Point (pI)

37.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-A20 PF01754 13 - 34 5.9e-11 A20-like zinc finger
zf-AN1 PF01428 89 - 129 1.3e-07 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 189
Acc16I TGCGCA 1 cut(s) 43
AccB1I GGYRCC 1 cut(s) 264
AccII CGCG 1 cut(s) 148
AciI CCGC 2 cut(s) 54, 148
AcuI CTGAAG 1 cut(s) 150
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 1 cut(s) 220
AflII CTTAAG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 371
AgsI TTSAA 1 cut(s) 216
AleI CACNNNNGTG 1 cut(s) 327
AluBI AGCT 3 cut(s) 124, 273, 406
AluI AGCT 3 cut(s) 124, 273, 406
Alw21I GWGCWC 1 cut(s) 92
Alw26I GTCTC 1 cut(s) 12
ApeKI GCWGC 2 cut(s) 143, 273
Asp700I GAANNNNTTC 1 cut(s) 123
AspLEI GCGC 2 cut(s) 44, 148
AsuNHI GCTAGC 1 cut(s) 200
BanI GGYRCC 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 92
BbvI GCAGC 2 cut(s) 155, 260
BclI TGATCA 1 cut(s) 232
BcoDI GTCTC 1 cut(s) 12
BfaI CTAG 3 cut(s) 182, 201, 442
BfmI CTRYAG 1 cut(s) 141
BfrI CTTAAG 1 cut(s) 293
BisI GCNGC 2 cut(s) 144, 274
BlpI GCTNAGC 1 cut(s) 132
BlsI GCNGC 2 cut(s) 145, 275
BmcAI AGTACT 1 cut(s) 220
BmiI GGNNCC 1 cut(s) 266
BmrI ACTGGG 1 cut(s) 419
BmtI GCTAGC 1 cut(s) 204
BmuI ACTGGG 1 cut(s) 419
BpiI GAAGAC 1 cut(s) 204
Bpu1102I GCTNAGC 1 cut(s) 132
BsaHI GRCGYC 1 cut(s) 186
Bse118I RCCGGY 1 cut(s) 298
Bse1I ACTGG 1 cut(s) 425
BseNI ACTGG 1 cut(s) 425
BseXI GCAGC 2 cut(s) 155, 260
BsgI GTGCAG 1 cut(s) 363
Bsh1236I CGCG 1 cut(s) 148
BshNI GGYRCC 1 cut(s) 264
BsiHKAI GWGCWC 1 cut(s) 92
BsiSI CCGG 1 cut(s) 299
BslFI GGGAC 2 cut(s) 12, 79
BsmAI GTCTC 1 cut(s) 12
BsmFI GGGAC 2 cut(s) 12, 79
Bsp1286I GDGCHC 1 cut(s) 92
Bsp143I GATC 1 cut(s) 232
Bsp1720I GCTNAGC 1 cut(s) 132
BspACI CCGC 2 cut(s) 54, 148
BspFNI CGCG 1 cut(s) 148
BspLI GGNNCC 1 cut(s) 266
BspMAI CTGCAG 1 cut(s) 145
BspOI GCTAGC 1 cut(s) 204
BspT107I GGYRCC 1 cut(s) 264
BspTI CTTAAG 1 cut(s) 293
BsrFI RCCGGY 1 cut(s) 298
BsrI ACTGG 1 cut(s) 425
BssAI RCCGGY 1 cut(s) 298
BssMI GATC 1 cut(s) 232
BssNI GRCGYC 1 cut(s) 186
Bst4CI ACNGT 2 cut(s) 26, 326
BstACI GRCGYC 1 cut(s) 186
BstAFI CTTAAG 1 cut(s) 293
BstC8I GCNNGC 3 cut(s) 150, 202, 300
BstDEI CTNAG 1 cut(s) 132
BstFNI CGCG 1 cut(s) 148
BstHHI GCGC 2 cut(s) 44, 148
BstKTI GATC 1 cut(s) 235
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 1 cut(s) 232
BstMWI GCNNNNNNNGC 5 cut(s) 96, 149, 197, 273, 279
BstNSI RCATGY 1 cut(s) 375
BstSFI CTRYAG 1 cut(s) 141
BstUI CGCG 1 cut(s) 148
BstV1I GCAGC 2 cut(s) 155, 260
BstV2I GAAGAC 1 cut(s) 204
BstXI CCANNNNNNTGG 1 cut(s) 329
BtsIMutI CAGTG 2 cut(s) 174, 322
Cac8I GCNNGC 3 cut(s) 150, 202, 300
CfoI GCGC 2 cut(s) 44, 148
Cfr10I RCCGGY 1 cut(s) 298
CseI GACGC 1 cut(s) 227
Csp6I GTAC 1 cut(s) 219
CviAII CATG 2 cut(s) 19, 372
CviQI GTAC 1 cut(s) 219
DdeI CTNAG 1 cut(s) 132
DpnI GATC 1 cut(s) 234
DpnII GATC 1 cut(s) 232
Eco57I CTGAAG 1 cut(s) 150
FaeI CATG 2 cut(s) 22, 375
FaiI YATR 4 cut(s) 20, 86, 351, 373
FalI AAGNNNNNCTT 2 cut(s) 108, 140
FaqI GGGAC 2 cut(s) 12, 79
FatI CATG 2 cut(s) 18, 371
FauI CCCGC 1 cut(s) 141
FbaI TGATCA 1 cut(s) 232
Fnu4HI GCNGC 2 cut(s) 144, 274
Fsp4HI GCNGC 2 cut(s) 144, 274
FspBI CTAG 3 cut(s) 182, 201, 442
FspI TGCGCA 1 cut(s) 43
GlaI GCGC 2 cut(s) 43, 147
GluI GCNGC 2 cut(s) 144, 274
HapII CCGG 1 cut(s) 299
HgaI GACGC 1 cut(s) 227
HhaI GCGC 2 cut(s) 44, 148
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 2 cut(s) 22, 375
Hin6I GCGC 2 cut(s) 42, 146
HinP1I GCGC 2 cut(s) 42, 146
HindIII AAGCTT 1 cut(s) 122
HinfI GANTC 3 cut(s) 5, 206, 362
HpaII CCGG 1 cut(s) 299
Hpy188I TCNGA 1 cut(s) 114
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 1 cut(s) 385
HpyCH4III ACNGT 2 cut(s) 26, 326
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 5 cut(s) 143, 224, 282, 344, 432
HpyF10VI GCNNNNNNNGC 5 cut(s) 96, 149, 197, 273, 279
HpyF3I CTNAG 1 cut(s) 132
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 2 cut(s) 22, 375
HspAI GCGC 2 cut(s) 42, 146
KroI GCCGGC 1 cut(s) 298
KroNI GCCGGC 1 cut(s) 300
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 1 cut(s) 232
LpnPI CCDG 4 cut(s) 281, 312, 381, 438
Lsp1109I GCAGC 2 cut(s) 155, 260
MaeI CTAG 3 cut(s) 182, 201, 442
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 2 cut(s) 250, 437
MalI GATC 1 cut(s) 234
MboI GATC 1 cut(s) 232
MboII GAAGA 1 cut(s) 204
MhlI GDGCHC 1 cut(s) 92
MluCI AATT 1 cut(s) 382
MlyI GAGTC 2 cut(s) 14, 215
MnlI CCTC 1 cut(s) 42
MroNI GCCGGC 1 cut(s) 298
MroXI GAANNNNTTC 1 cut(s) 123
MseI TTAA 2 cut(s) 294, 314
MslI CAYNNNNRTG 2 cut(s) 316, 327
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 1 cut(s) 299
MvnI CGCG 1 cut(s) 148
MwoI GCNNNNNNNGC 5 cut(s) 96, 149, 197, 273, 279
NaeI GCCGGC 1 cut(s) 300
NdeII GATC 1 cut(s) 232
NgoMIV GCCGGC 1 cut(s) 298
NheI GCTAGC 1 cut(s) 200
NlaIII CATG 2 cut(s) 22, 375
NlaIV GGNNCC 1 cut(s) 266
NmuCI GTSAC 2 cut(s) 250, 437
NsbI TGCGCA 1 cut(s) 43
NspI RCATGY 1 cut(s) 375
OliI CACNNNNGTG 1 cut(s) 327
PciI ACATGT 1 cut(s) 371
PdiI GCCGGC 1 cut(s) 300
PdmI GAANNNNTTC 1 cut(s) 123
PfeI GAWTC 1 cut(s) 362
PkrI GCNGC 2 cut(s) 145, 275
PleI GAGTC 2 cut(s) 13, 214
PpsI GAGTC 2 cut(s) 13, 214
PscI ACATGT 1 cut(s) 371
PspN4I GGNNCC 1 cut(s) 266
PstI CTGCAG 1 cut(s) 145
RsaI GTAC 1 cut(s) 220
RsaNI GTAC 1 cut(s) 219
RseI CAYNNNNRTG 2 cut(s) 316, 327
SaqAI TTAA 2 cut(s) 294, 314
SatI GCNGC 2 cut(s) 144, 274
Sau3AI GATC 1 cut(s) 232
ScaI AGTACT 1 cut(s) 220
SchI GAGTC 2 cut(s) 14, 215
SduI GDGCHC 1 cut(s) 92
SetI ASST 6 cut(s) 126, 189, 266, 275, 356, 408
SfcI CTRYAG 1 cut(s) 141
SmiMI CAYNNNNRTG 2 cut(s) 316, 327
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
Sse9I AATT 1 cut(s) 382
SsiI CCGC 2 cut(s) 54, 148
SspMI CTAG 3 cut(s) 182, 201, 442
TaaI ACNGT 2 cut(s) 26, 326
TaiI ACGT 1 cut(s) 189
TasI AATT 1 cut(s) 382
TatI WGTACW 1 cut(s) 218
TfiI GAWTC 1 cut(s) 362
Tru1I TTAA 2 cut(s) 294, 314
Tru9I TTAA 2 cut(s) 294, 314
TscAI CASTG 2 cut(s) 174, 329
TseFI GTSAC 2 cut(s) 250, 437
TseI GCWGC 2 cut(s) 143, 273
Tsp45I GTSAC 2 cut(s) 250, 437
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 2 cut(s) 174, 329
Vha464I CTTAAG 1 cut(s) 293
XceI RCATGY 1 cut(s) 375
XmnI GAANNNNTTC 1 cut(s) 123
XspI CTAG 3 cut(s) 182, 201, 442
ZraI GACGTC 1 cut(s) 187
ZrmI AGTACT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.