Rroxscaffold_4G00282940

zinc ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002517
Physical Location & Seq
Forward (+)
5020449 .. 5021062
614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00282940.1

Sequence Viewer

Length: 516 bp
ATGAAATACTACTTACGAGCGAAGATCAAAGAAGAAGGAGAATCAATTCAGAAGCAAAAGTATGGACTCGCCAAGAAACATGGACTGTCCTCCACCACTGTGTGCAAGAGGTTGCGGTTTCTTTGGGACTTCGGCAAACAGGAATATGTGCTCAAAGTGCTACACAGACTATCTGAAGGAACAGCTTCTTGCGAAGCCTACTGCAGCGCCGGCCGTGGTGGCTTCAGTGAAAAAACCCTAGACGTCACTGTTTCTGCTAGCGAGTCGTTGTCAACTACTACAACTACTGATCAGCCATGTGGTGCGACGAAGAACAAGTGCCAGGATGAGAGCTGCAAGAAGAAACTTAACCTGGTGGAAATGATGACTTTCAAATGCCGCTGTGGTGGAGTGTTTTGTACGAAGCATAAGTATCCTGAACACTCATGTAGTGTGAATTACAAGAAGGCTGAACGAGAGCTTTTGGCCAAGCAAAACCCAATTTGCAAGGGTGACAAGTTAGAGTATAGGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-A20 zf-AN1

Protein Analysis

171

Amino Acids

19.33

Weight (kDa)

9.26

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-AN1 PF01428 107 - 147 2.2e-07 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 246
AciI CCGC 2 cut(s) 115, 379
AcoI YGGCCR 2 cut(s) 211, 465
AcuI CTGAAG 2 cut(s) 195, 208
AcyI GRCGYC 1 cut(s) 243
AdeI CACNNNGTG 1 cut(s) 102
AfaI GTAC 1 cut(s) 400
AgsI TTSAA 1 cut(s) 373
AjnI CCWGG 2 cut(s) 321, 351
AleI CACNNNNGTG 1 cut(s) 98
AluBI AGCT 3 cut(s) 185, 333, 460
AluI AGCT 3 cut(s) 185, 333, 460
Alw21I GWGCWC 1 cut(s) 153
AoxI GGCC 2 cut(s) 211, 465
ApeKI GCWGC 2 cut(s) 204, 333
Asp700I GAANNNNTTC 2 cut(s) 45, 184
AspLEI GCGC 1 cut(s) 209
AsuHPI GGTGA 1 cut(s) 503
AsuNHI GCTAGC 1 cut(s) 257
BalI TGGCCA 1 cut(s) 467
Bbv12I GWGCWC 1 cut(s) 153
BbvI GCAGC 2 cut(s) 216, 320
BceAI ACGGC 1 cut(s) 198
BciT130I CCWGG 2 cut(s) 323, 353
BciVI GTATCC 1 cut(s) 423
BclI TGATCA 1 cut(s) 289
BfaI CTAG 2 cut(s) 239, 258
BfmI CTRYAG 1 cut(s) 202
BfoI RGCGCY 1 cut(s) 210
BfuI GTATCC 1 cut(s) 423
BglI GCCNNNNNGGC 1 cut(s) 219
BisI GCNGC 3 cut(s) 205, 334, 379
BlsI GCNGC 3 cut(s) 206, 335, 380
Bme1390I CCNGG 2 cut(s) 323, 353
BmrFI CCNGG 2 cut(s) 323, 353
BmtI GCTAGC 1 cut(s) 261
BsaHI GRCGYC 1 cut(s) 243
BsaJI CCNNGG 1 cut(s) 214
Bse118I RCCGGY 1 cut(s) 209
BseBI CCWGG 2 cut(s) 323, 353
BseDI CCNNGG 1 cut(s) 214
BseGI GGATG 1 cut(s) 331
BseX3I CGGCCG 1 cut(s) 211
BseXI GCAGC 2 cut(s) 216, 320
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 2 cut(s) 213, 467
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 153
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 140
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 2 cut(s) 213, 467
Bsp1286I GDGCHC 1 cut(s) 153
Bsp143I GATC 2 cut(s) 24, 289
BspACI CCGC 2 cut(s) 115, 379
BspANI GGCC 2 cut(s) 213, 467
BspMAI CTGCAG 1 cut(s) 206
BspOI GCTAGC 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 209
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 2 cut(s) 24, 289
BssNI GRCGYC 1 cut(s) 243
Bst2UI CCWGG 2 cut(s) 323, 353
Bst4CI ACNGT 3 cut(s) 87, 100, 250
BstACI GRCGYC 1 cut(s) 243
BstC8I GCNNGC 2 cut(s) 211, 259
BstDSI CCRYGG 1 cut(s) 214
BstF5I GGATG 1 cut(s) 331
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 1 cut(s) 209
BstKTI GATC 2 cut(s) 27, 292
BstMBI GATC 2 cut(s) 24, 289
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 3 cut(s) 157, 210, 219
BstNI CCWGG 2 cut(s) 323, 353
BstSCI CCNGG 2 cut(s) 321, 351
BstSFI CTRYAG 1 cut(s) 202
BstV1I GCAGC 2 cut(s) 216, 320
BstZI CGGCCG 1 cut(s) 211
BsuI GTATCC 1 cut(s) 423
BsuRI GGCC 2 cut(s) 213, 467
BtgI CCRYGG 1 cut(s) 214
BtsCI GGATG 1 cut(s) 331
BtsIMutI CAGTG 3 cut(s) 96, 232, 246
Cac8I GCNNGC 2 cut(s) 211, 259
CfoI GCGC 1 cut(s) 209
Cfr10I RCCGGY 1 cut(s) 209
CsiI ACCWGGT 1 cut(s) 351
Csp6I GTAC 1 cut(s) 399
CviAII CATG 3 cut(s) 80, 297, 426
CviJI RGCY 9 cut(s) 185, 197, 213, 222, 295, 333, 449, 460, 467
CviKI_1 RGCY 9 cut(s) 185, 197, 213, 222, 295, 333, 449, 460, 467
CviQI GTAC 1 cut(s) 399
DpnI GATC 2 cut(s) 26, 291
DpnII GATC 2 cut(s) 24, 289
DraIII CACNNNGTG 1 cut(s) 102
EaeI YGGCCR 2 cut(s) 211, 465
EagI CGGCCG 1 cut(s) 211
EclXI CGGCCG 1 cut(s) 211
Eco52I CGGCCG 1 cut(s) 211
Eco57I CTGAAG 2 cut(s) 195, 208
EcoRII CCWGG 2 cut(s) 321, 351
FaeI CATG 3 cut(s) 83, 300, 429
FaiI YATR 7 cut(s) 63, 81, 147, 298, 408, 427, 507
FaqI GGGAC 1 cut(s) 140
FatI CATG 3 cut(s) 79, 296, 425
FbaI TGATCA 1 cut(s) 289
Fnu4HI GCNGC 3 cut(s) 205, 334, 379
FokI GGATG 1 cut(s) 338
Fsp4HI GCNGC 3 cut(s) 205, 334, 379
FspBI CTAG 2 cut(s) 239, 258
GlaI GCGC 1 cut(s) 208
GluI GCNGC 3 cut(s) 205, 334, 379
HaeII RGCGCY 1 cut(s) 210
HaeIII GGCC 2 cut(s) 213, 467
HapII CCGG 1 cut(s) 210
HhaI GCGC 1 cut(s) 209
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 3 cut(s) 83, 300, 429
Hin6I GCGC 1 cut(s) 207
HinP1I GCGC 1 cut(s) 207
HincII GTYRAC 1 cut(s) 273
HindII GTYRAC 1 cut(s) 273
HinfI GANTC 3 cut(s) 41, 66, 263
HpaII CCGG 1 cut(s) 210
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 1 cut(s) 273
Hpy188I TCNGA 2 cut(s) 51, 175
Hpy188III TCNNGA 1 cut(s) 416
Hpy8I GTNNAC 1 cut(s) 273
Hpy99I CGWCG 1 cut(s) 310
HpyAV CCTTC 3 cut(s) 29, 170, 439
HpyCH4III ACNGT 3 cut(s) 87, 100, 250
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 4 cut(s) 105, 204, 336, 486
HpyF10VI GCNNNNNNNGC 3 cut(s) 157, 210, 219
HpySE526I ACGT 1 cut(s) 243
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 3 cut(s) 83, 300, 429
HspAI GCGC 1 cut(s) 207
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Ksp22I TGATCA 1 cut(s) 289
Kzo9I GATC 2 cut(s) 24, 289
LpnPI CCDG 7 cut(s) 125, 223, 308, 335, 338, 365, 429
Lsp1109I GCAGC 2 cut(s) 216, 320
MabI ACCWGGT 1 cut(s) 351
MaeI CTAG 2 cut(s) 239, 258
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 2 cut(s) 244, 491
MalI GATC 2 cut(s) 26, 291
MboI GATC 2 cut(s) 24, 289
MboII GAAGA 4 cut(s) 34, 44, 322, 352
MhlI GDGCHC 1 cut(s) 153
MlsI TGGCCA 1 cut(s) 467
MluCI AATT 3 cut(s) 45, 436, 480
MluNI TGGCCA 1 cut(s) 467
MlyI GAGTC 2 cut(s) 60, 272
MnlI CCTC 2 cut(s) 100, 102
Mox20I TGGCCA 1 cut(s) 467
MroNI GCCGGC 1 cut(s) 209
MroXI GAANNNNTTC 2 cut(s) 45, 184
MscI TGGCCA 1 cut(s) 467
MseI TTAA 1 cut(s) 348
MslI CAYNNNNRTG 1 cut(s) 98
Msp20I TGGCCA 1 cut(s) 467
MspA1I CMGCKG 1 cut(s) 381
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 2 cut(s) 323, 353
MvaI CCWGG 2 cut(s) 323, 353
MwoI GCNNNNNNNGC 3 cut(s) 157, 210, 219
NaeI GCCGGC 1 cut(s) 211
NdeII GATC 2 cut(s) 24, 289
NgoMIV GCCGGC 1 cut(s) 209
NheI GCTAGC 1 cut(s) 257
NlaIII CATG 3 cut(s) 83, 300, 429
NmuCI GTSAC 2 cut(s) 244, 491
OliI CACNNNNGTG 1 cut(s) 98
PdiI GCCGGC 1 cut(s) 211
PdmI GAANNNNTTC 2 cut(s) 45, 184
PfeI GAWTC 1 cut(s) 41
PkrI GCNGC 3 cut(s) 206, 335, 380
PleI GAGTC 2 cut(s) 60, 271
PpsI GAGTC 2 cut(s) 60, 271
Psp6I CCWGG 2 cut(s) 321, 351
PspGI CCWGG 2 cut(s) 321, 351
PstI CTGCAG 1 cut(s) 206
RsaI GTAC 1 cut(s) 400
RsaNI GTAC 1 cut(s) 399
RseI CAYNNNNRTG 1 cut(s) 98
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 3 cut(s) 205, 334, 379
Sau3AI GATC 2 cut(s) 24, 289
SchI GAGTC 2 cut(s) 60, 272
ScrFI CCNGG 2 cut(s) 323, 353
SduI GDGCHC 1 cut(s) 153
SetI ASST 6 cut(s) 113, 187, 246, 335, 354, 462
SexAI ACCWGGT 1 cut(s) 351
SfcI CTRYAG 1 cut(s) 202
SmiMI CAYNNNNRTG 1 cut(s) 98
Sse9I AATT 3 cut(s) 45, 436, 480
SsiI CCGC 2 cut(s) 115, 379
SspMI CTAG 2 cut(s) 239, 258
StyD4I CCNGG 2 cut(s) 321, 351
TaaI ACNGT 3 cut(s) 87, 100, 250
TaiI ACGT 1 cut(s) 246
TasI AATT 3 cut(s) 45, 436, 480
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TscAI CASTG 3 cut(s) 103, 232, 253
TseFI GTSAC 2 cut(s) 244, 491
TseI GCWGC 2 cut(s) 204, 333
Tsp45I GTSAC 2 cut(s) 244, 491
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 103, 232, 253
XmnI GAANNNNTTC 2 cut(s) 45, 184
XspI CTAG 2 cut(s) 239, 258
ZraI GACGTC 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.