Rmu_sc0000571.1_g000010

N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000571.1
OG0001436
Physical Location & Seq
Forward (+)
50603 .. 54357
3755 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 846 bp
atgaaggagtttcaagatccccaggctagggattccattgaaggtgcatttgagatggcgtggttattaattatctcttctatgattgtaatttggatagcttttctgttcaaaattctccatggtgcatactcaccttccaagggtgctttcgtgcatgattctaataatggtggcagtgccaagaagagaaatttgttgctggttgtttcccacccagatgatgagtccatgttcttcactccaacaataaactacctcacttcgagagggcattatattcacatcttatgctttcgctgcgagattgagttgaagatttcttctgaaggtgtccaactcagtaatccaaaaccagaaagagtacaaatagcagaggaggcagatgataatgatgatggagaaaaagaaaaagatgccgactgttggcacgataagcctgcaagcaagctcaacgaaacaagtccagaaggccggtgtaagattgatctgccgctgcatatttgtgaaatgcttgggatgcttaccatgaagaatgaggaggcatatagtggatgcccttttttaagctacttttctgaagcttttcaagacacatattactatttcaggaggtcaaagcattttatggggcgaattgatgatgggatcatggggagagtgtcgaagccaagttccgcgctttgccaatacggcacagcaaaatacggcactatgacgcaaaaatccgaacgcaaacacggcatattctgtcaatttcattatattgttgggctgattggtgcaaggcagagaacacagaggctacttgaatatttagaagatcagttggttaaagctgcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PIG-L

Protein Analysis

281

Amino Acids

32.07

Weight (kDa)

6.07

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-L PF02585 67 - 99 2.3e-06 GlcNAc-PI de-N-acetylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 680
AciI CCGC 2 cut(s) 494, 678
AclWI GGATC 2 cut(s) 11, 656
AcsI RAATTY 2 cut(s) 114, 193
AcuI CTGAAG 2 cut(s) 348, 600
AfaI GTAC 1 cut(s) 366
AfiI CCNNNNNNNGG 4 cut(s) 27, 28, 143, 426
AgsI TTSAA 6 cut(s) 14, 41, 112, 316, 590, 812
AjnI CCWGG 1 cut(s) 21
AjuI GAANNNNNNNTTGG 2 cut(s) 134, 166
AluBI AGCT 5 cut(s) 101, 451, 570, 584, 839
AluI AGCT 5 cut(s) 101, 451, 570, 584, 839
AlwI GGATC 2 cut(s) 11, 656
AoxI GGCC 1 cut(s) 472
ApeKI GCWGC 3 cut(s) 300, 496, 839
ApoI RAATTY 2 cut(s) 114, 193
AseI ATTAAT 1 cut(s) 68
Asp700I GAANNNNTTC 1 cut(s) 585
AspLEI GCGC 1 cut(s) 682
AsuHPI GGTGA 1 cut(s) 126
BbvI GCAGC 3 cut(s) 287, 483, 826
BccI CCATC 3 cut(s) 49, 392, 638
BceAI ACGGC 3 cut(s) 709, 724, 757
BcgI CGANNNNNNTGC 2 cut(s) 422, 456
BciT130I CCWGG 1 cut(s) 23
BfaI CTAG 1 cut(s) 27
BglI GCCNNNNNGGC 1 cut(s) 693
BisI GCNGC 4 cut(s) 301, 494, 497, 840
BlsI GCNGC 4 cut(s) 302, 495, 498, 841
Bme1390I CCNGG 1 cut(s) 23
BmrFI CCNGG 1 cut(s) 23
BmsI GCATC 3 cut(s) 406, 510, 545
BsaJI CCNNGG 3 cut(s) 21, 121, 141
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 28, 143, 426
Bse118I RCCGGY 1 cut(s) 474
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 3 cut(s) 21, 121, 141
BseGI GGATG 2 cut(s) 525, 560
BseLI CCNNNNNNNGG 4 cut(s) 27, 28, 143, 426
BseMII CTCAG 1 cut(s) 355
BseRI GAGGAG 2 cut(s) 392, 554
BseXI GCAGC 3 cut(s) 287, 483, 826
Bsh1236I CGCG 1 cut(s) 680
BshFI GGCC 1 cut(s) 474
BsiSI CCGG 1 cut(s) 475
BslI CCNNNNNNNGG 4 cut(s) 27, 28, 143, 426
BsnI GGCC 1 cut(s) 474
Bsp143I GATC 4 cut(s) 16, 487, 648, 823
Bsp19I CCATGG 1 cut(s) 121
BspACI CCGC 2 cut(s) 494, 678
BspANI GGCC 1 cut(s) 474
BspCNI CTCAG 1 cut(s) 354
BspFNI CGCG 1 cut(s) 680
BspPI GGATC 2 cut(s) 11, 656
BsrFI RCCGGY 1 cut(s) 474
BssAI RCCGGY 1 cut(s) 474
BssECI CCNNGG 3 cut(s) 21, 121, 141
BssMI GATC 4 cut(s) 16, 487, 648, 823
BssT1I CCWWGG 2 cut(s) 121, 141
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 1 cut(s) 425
Bst6I CTCTTC 2 cut(s) 82, 182
BstC8I GCNNGC 3 cut(s) 441, 445, 449
BstDEI CTNAG 1 cut(s) 341
BstDSI CCRYGG 1 cut(s) 121
BstF5I GGATG 2 cut(s) 525, 560
BstFNI CGCG 1 cut(s) 680
BstHHI GCGC 1 cut(s) 682
BstKTI GATC 4 cut(s) 19, 490, 651, 826
BstMBI GATC 4 cut(s) 16, 487, 648, 823
BstMWI GCNNNNNNNGC 6 cut(s) 300, 380, 436, 520, 693, 741
BstNI CCWGG 1 cut(s) 23
BstSCI CCNGG 1 cut(s) 21
BstUI CGCG 1 cut(s) 680
BstV1I GCAGC 3 cut(s) 287, 483, 826
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
BsuRI GGCC 1 cut(s) 474
BtgI CCRYGG 1 cut(s) 121
BtsCI GGATG 2 cut(s) 525, 560
BtsI GCAGTG 1 cut(s) 184
BtsIMutI CAGTG 1 cut(s) 184
Cac8I GCNNGC 3 cut(s) 441, 445, 449
CfoI GCGC 1 cut(s) 682
Cfr10I RCCGGY 1 cut(s) 474
CseI GACGC 1 cut(s) 727
Csp6I GTAC 1 cut(s) 365
CviAII CATG 6 cut(s) 122, 158, 232, 529, 652, 843
CviQI GTAC 1 cut(s) 365
DdeI CTNAG 1 cut(s) 341
DpnI GATC 4 cut(s) 18, 489, 650, 825
DpnII GATC 4 cut(s) 16, 487, 648, 823
Eam1104I CTCTTC 2 cut(s) 82, 182
EarI CTCTTC 2 cut(s) 82, 182
Eco130I CCWWGG 2 cut(s) 121, 141
Eco57I CTGAAG 2 cut(s) 348, 600
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 2 cut(s) 121, 141
ErhI CCWWGG 2 cut(s) 121, 141
FaeI CATG 6 cut(s) 125, 161, 235, 532, 655, 846
FatI CATG 6 cut(s) 121, 157, 231, 528, 651, 842
Fnu4HI GCNGC 4 cut(s) 301, 494, 497, 840
FokI GGATG 2 cut(s) 532, 567
Fsp4HI GCNGC 4 cut(s) 301, 494, 497, 840
FspBI CTAG 1 cut(s) 27
GlaI GCGC 1 cut(s) 681
GluI GCNGC 4 cut(s) 301, 494, 497, 840
HaeIII GGCC 1 cut(s) 474
HapII CCGG 1 cut(s) 475
HgaI GACGC 1 cut(s) 727
HhaI GCGC 1 cut(s) 682
Hin1II CATG 6 cut(s) 125, 161, 235, 532, 655, 846
Hin6I GCGC 1 cut(s) 680
HinP1I GCGC 1 cut(s) 680
HindIII AAGCTT 1 cut(s) 582
HinfI GANTC 3 cut(s) 32, 161, 227
HpaII CCGG 1 cut(s) 475
HphI GGTGA 1 cut(s) 126
Hpy188I TCNGA 3 cut(s) 328, 580, 730
Hpy188III TCNNGA 5 cut(s) 14, 267, 467, 590, 610
HpyAV CCTTC 4 cut(s) 35, 147, 323, 464
HpyCH4III ACNGT 1 cut(s) 425
HpyCH4V TGCA 7 cut(s) 47, 128, 157, 443, 499, 785, 842
HpyF10VI GCNNNNNNNGC 6 cut(s) 300, 380, 436, 520, 693, 741
HpyF3I CTNAG 1 cut(s) 341
Hsp92II CATG 6 cut(s) 125, 161, 235, 532, 655, 846
HspAI GCGC 1 cut(s) 680
Kzo9I GATC 4 cut(s) 16, 487, 648, 823
LpnPI CCDG 9 cut(s) 8, 35, 188, 231, 369, 453, 480, 488, 595
Lsp1109I GCAGC 3 cut(s) 287, 483, 826
LweI GCATC 3 cut(s) 406, 510, 545
MaeI CTAG 1 cut(s) 27
MalI GATC 4 cut(s) 18, 489, 650, 825
MboI GATC 4 cut(s) 16, 487, 648, 823
MboII GAAGA 7 cut(s) 69, 199, 229, 315, 328, 544, 833
MflI RGATCY 1 cut(s) 16
MluCI AATT 6 cut(s) 69, 90, 114, 193, 636, 755
MlyI GAGTC 1 cut(s) 236
MmeI TCCRAC 2 cut(s) 269, 361
MnlI CCTC 8 cut(s) 263, 269, 370, 373, 532, 535, 606, 795
MroXI GAANNNNTTC 1 cut(s) 585
MseI TTAA 3 cut(s) 68, 566, 834
MslI CAYNNNNRTG 2 cut(s) 219, 504
MspA1I CMGCKG 1 cut(s) 496
MspI CCGG 1 cut(s) 475
MspR9I CCNGG 1 cut(s) 23
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 1 cut(s) 680
MwoI GCNNNNNNNGC 6 cut(s) 300, 380, 436, 520, 693, 741
NcoI CCATGG 1 cut(s) 121
NdeII GATC 4 cut(s) 16, 487, 648, 823
NlaIII CATG 6 cut(s) 125, 161, 235, 532, 655, 846
PdmI GAANNNNTTC 1 cut(s) 585
PfeI GAWTC 2 cut(s) 32, 161
PkrI GCNGC 4 cut(s) 302, 495, 498, 841
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
PshBI ATTAAT 1 cut(s) 68
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PsuI RGATCY 1 cut(s) 16
RsaI GTAC 1 cut(s) 366
RsaNI GTAC 1 cut(s) 365
RseI CAYNNNNRTG 2 cut(s) 219, 504
SaqAI TTAA 3 cut(s) 68, 566, 834
SatI GCNGC 4 cut(s) 301, 494, 497, 840
Sau3AI GATC 4 cut(s) 16, 487, 648, 823
SchI GAGTC 1 cut(s) 236
ScrFI CCNGG 1 cut(s) 23
SfaNI GCATC 3 cut(s) 406, 510, 545
SmiMI CAYNNNNRTG 2 cut(s) 219, 504
Sse9I AATT 6 cut(s) 69, 90, 114, 193, 636, 755
SsiI CCGC 2 cut(s) 494, 678
SspI AATATT 1 cut(s) 815
SspMI CTAG 1 cut(s) 27
StyD4I CCNGG 1 cut(s) 21
StyI CCWWGG 2 cut(s) 121, 141
TaaI ACNGT 1 cut(s) 425
TaqI TCGA 2 cut(s) 266, 665
TasI AATT 6 cut(s) 69, 90, 114, 193, 636, 755
TatI WGTACW 1 cut(s) 364
TauI GCSGC 1 cut(s) 496
TfiI GAWTC 2 cut(s) 32, 161
Tru1I TTAA 3 cut(s) 68, 566, 834
Tru9I TTAA 3 cut(s) 68, 566, 834
TscAI CASTG 1 cut(s) 184
TseI GCWGC 3 cut(s) 300, 496, 839
TspDTI ATGAA 3 cut(s) 17, 545, 749
TspRI CASTG 1 cut(s) 184
VspI ATTAAT 1 cut(s) 68
XapI RAATTY 2 cut(s) 114, 193
XmnI GAANNNNTTC 1 cut(s) 585
XspI CTAG 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.