Rmu_sc0002269.1_g000009
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002269.1
OG0005560
Physical Location & Seq
Reverse (-)
32099 .. 33992
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1092 bp
atggctggaattgaaggtcatgcaatcggaattgatctcggcacaaagtactcatgtgtgggagtttggaagcatgataacaataatgtggaaatcatagtcaacgatcagggaaacaggaccacgccgtcatgtgttgccttcactgatactgagcttttggtaggagaagcagcgtttaatcaggtcgtaagaaaccctgacaactccatcttcgatgcaaagcggttaattggccggaaattcagtgatgcatctgttcaaagtgacaagaagctctggccattcaaggtggaatgcttcccagtttcagaagcttctgatgccatcttggtgcggcgcgtggcggcgcgtcggtggcgacctggagggagtcccccactccccggcgaagatagacctccggattggtcggcaaattgtttgatgagggggagctgcacggagcagaggggacggtctgacggtaacgccgcgtgccggtcgttgtcagtgaggcgtggcctcgggattttccatttggcggacatccccccgccggatgcggcaggaaacagcgtccggatagcaagggaggagttccgctggcggggtttcgctcagcggagacttcgtcacttggaaaatgacaagggagaagttccgctagcgggagaagttccgctggcggtgttcgctcagcggagacttcgttacttggaaaatgacaagggagaagttccgttggcggtgtttcgctcagcggagacctcgtcacttggaaaatgccaagggagaagttccgttggcggtgtttcgctcagcggagacttcgtcacttggaaaatgacaagggagacgttccgctggcggtgtttcgctcagcggagacttcgttacttggaaaatgacaagggagaagttccgctagcggtgtttcactcagcggagacctcgtcacttggaaaatgccaagggagaagttccgttggcggtgtttcgctcagcggagacttcgtcacttggaaaagacaagggagaagttccgctggcggtgtttcgctcagcggagacttcgttacttggaaaatgacaagggagaagttccgctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

363

Amino Acids

40.15

Weight (kDa)

9.62

Isoelectric Point (pI)

52.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 8 - 99 2.4e-34 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 127
AccII CGCG 3 cut(s) 342, 352, 476
AccIII TCCGGA 2 cut(s) 403, 561
AcoI YGGCCR 2 cut(s) 235, 281
AcsI RAATTY 1 cut(s) 242
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 6 cut(s) 386, 480, 523, 538, 589, 650
AgsI TTSAA 3 cut(s) 14, 263, 289
AjnI CCWGG 1 cut(s) 364
AluBI AGCT 4 cut(s) 157, 277, 317, 438
AluI AGCT 4 cut(s) 157, 277, 317, 438
Alw26I GTCTC 9 cut(s) 601, 679, 740, 801, 830, 862, 923, 984, 1044
Ama87I CYCGRG 1 cut(s) 506
Aor13HI TCCGGA 2 cut(s) 403, 561
AoxI GGCC 3 cut(s) 235, 281, 502
ApeKI GCWGC 2 cut(s) 173, 438
ApoI RAATTY 1 cut(s) 242
AspLEI GCGC 2 cut(s) 342, 352
AspS9I GGNCC 1 cut(s) 120
AsuC2I CCSGG 1 cut(s) 387
AsuNHI GCTAGC 2 cut(s) 646, 907
AvaI CYCGRG 1 cut(s) 506
AvaII GGWCC 1 cut(s) 120
BalI TGGCCA 1 cut(s) 283
BbvI GCAGC 2 cut(s) 185, 425
BccI CCATC 2 cut(s) 218, 335
BceAI ACGGC 1 cut(s) 112
BciT130I CCWGG 1 cut(s) 366
BcnI CCSGG 1 cut(s) 387
BcoDI GTCTC 9 cut(s) 601, 679, 740, 801, 830, 862, 923, 984, 1044
BfaI CTAG 3 cut(s) 647, 908, 1090
BisI GCNGC 6 cut(s) 174, 338, 348, 439, 474, 546
BlpI GCTNAGC 7 cut(s) 600, 678, 739, 800, 861, 983, 1043
BlsI GCNGC 6 cut(s) 175, 339, 349, 440, 475, 547
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 2 cut(s) 366, 387
Bme18I GGWCC 1 cut(s) 120
BmeT110I CYCGRG 1 cut(s) 506
BmgT120I GGNCC 1 cut(s) 120
BmrFI CCNGG 2 cut(s) 366, 387
BmrI ACTGGG 1 cut(s) 299
BmsI GCATC 5 cut(s) 208, 241, 263, 313, 532
BmtI GCTAGC 2 cut(s) 650, 911
BmuI ACTGGG 1 cut(s) 299
BpmI CTGGAG 1 cut(s) 387
Bpu1102I GCTNAGC 7 cut(s) 600, 678, 739, 800, 861, 983, 1043
BpuMI CCSGG 1 cut(s) 387
BsaI GGTCTC 2 cut(s) 740, 923
BsaJI CCNNGG 4 cut(s) 385, 505, 769, 952
BsaWI WCCGGW 2 cut(s) 403, 561
Bsc4I CCNNNNNNNGG 6 cut(s) 386, 480, 523, 538, 589, 650
Bse118I RCCGGY 1 cut(s) 480
Bse1I ACTGG 1 cut(s) 305
BseAI TCCGGA 2 cut(s) 403, 561
BseBI CCWGG 1 cut(s) 366
BseDI CCNNGG 4 cut(s) 385, 505, 769, 952
BseGI GGATG 2 cut(s) 528, 547
BseLI CCNNNNNNNGG 6 cut(s) 386, 480, 523, 538, 589, 650
BseMII CTCAG 9 cut(s) 144, 614, 692, 753, 814, 875, 936, 997, 1057
BseNI ACTGG 1 cut(s) 305
BseRI GAGGAG 1 cut(s) 590
BseXI GCAGC 2 cut(s) 185, 425
BsgI GTGCAG 1 cut(s) 424
Bsh1236I CGCG 3 cut(s) 342, 352, 476
Bsh1285I CGRYCG 1 cut(s) 485
BshFI GGCC 3 cut(s) 237, 283, 504
BsiEI CGRYCG 1 cut(s) 485
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 6 cut(s) 238, 387, 404, 481, 539, 562
BslFI GGGAC 2 cut(s) 360, 468
BslI CCNNNNNNNGG 6 cut(s) 386, 480, 523, 538, 589, 650
BsmAI GTCTC 9 cut(s) 601, 679, 740, 801, 830, 862, 923, 984, 1044
BsmBI CGTCTC 1 cut(s) 830
BsmFI GGGAC 2 cut(s) 360, 468
BsmI GAATGC 1 cut(s) 302
BsnI GGCC 3 cut(s) 237, 283, 504
Bso31I GGTCTC 2 cut(s) 740, 923
BsoBI CYCGRG 1 cut(s) 506
Bsp13I TCCGGA 2 cut(s) 403, 561
Bsp143I GATC 2 cut(s) 34, 106
Bsp1720I GCTNAGC 7 cut(s) 600, 678, 739, 800, 861, 983, 1043
BspANI GGCC 3 cut(s) 237, 283, 504
BspCNI CTCAG 9 cut(s) 145, 613, 691, 752, 813, 874, 935, 996, 1056
BspEI TCCGGA 2 cut(s) 403, 561
BspFNI CGCG 3 cut(s) 342, 352, 476
BspOI GCTAGC 2 cut(s) 650, 911
BspTNI GGTCTC 2 cut(s) 740, 923
BsrFI RCCGGY 1 cut(s) 480
BsrI ACTGG 1 cut(s) 305
BssAI RCCGGY 1 cut(s) 480
BssECI CCNNGG 4 cut(s) 385, 505, 769, 952
BssMI GATC 2 cut(s) 34, 106
BssT1I CCWWGG 2 cut(s) 769, 952
Bst2UI CCWGG 1 cut(s) 366
Bst4CI ACNGT 2 cut(s) 459, 467
BstC8I GCNNGC 7 cut(s) 478, 587, 648, 666, 848, 909, 1030
BstDEI CTNAG 9 cut(s) 153, 600, 678, 739, 800, 861, 922, 983, 1043
BstF5I GGATG 2 cut(s) 528, 547
BstFNI CGCG 3 cut(s) 342, 352, 476
BstHHI GCGC 2 cut(s) 342, 352
BstKTI GATC 2 cut(s) 37, 109
BstMAI GTCTC 9 cut(s) 601, 679, 740, 801, 830, 862, 923, 984, 1044
BstMBI GATC 2 cut(s) 34, 106
BstMCI CGRYCG 1 cut(s) 485
BstMWI GCNNNNNNNGC 3 cut(s) 323, 358, 674
BstNI CCWGG 1 cut(s) 366
BstSCI CCNGG 2 cut(s) 364, 385
BstUI CGCG 3 cut(s) 342, 352, 476
BstV1I GCAGC 2 cut(s) 185, 425
BsuRI GGCC 3 cut(s) 237, 283, 504
BtsCI GGATG 2 cut(s) 528, 547
BtsIMutI CAGTG 3 cut(s) 144, 253, 498
Cac8I GCNNGC 7 cut(s) 478, 587, 648, 666, 848, 909, 1030
CfoI GCGC 2 cut(s) 342, 352
Cfr10I RCCGGY 1 cut(s) 480
Cfr13I GGNCC 1 cut(s) 120
CseI GACGC 2 cut(s) 341, 547
Csp6I GTAC 1 cut(s) 49
CviAII CATG 4 cut(s) 20, 54, 74, 132
CviJI RGCY 8 cut(s) 5, 157, 237, 277, 283, 317, 438, 504
CviKI_1 RGCY 8 cut(s) 5, 157, 237, 277, 283, 317, 438, 504
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 9 cut(s) 153, 600, 678, 739, 800, 861, 922, 983, 1043
DpnI GATC 2 cut(s) 36, 108
DpnII GATC 2 cut(s) 34, 106
DrdI GACNNNNNNGTC 1 cut(s) 127
DseDI GACNNNNNNGTC 1 cut(s) 127
EaeI YGGCCR 2 cut(s) 235, 281
EciI GGCGGA 1 cut(s) 539
Eco130I CCWWGG 2 cut(s) 769, 952
Eco31I GGTCTC 2 cut(s) 740, 923
Eco47I GGWCC 1 cut(s) 120
Eco88I CYCGRG 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 364
EcoT14I CCWWGG 2 cut(s) 769, 952
EcoT22I ATGCAT 1 cut(s) 256
ErhI CCWWGG 2 cut(s) 769, 952
Esp3I CGTCTC 1 cut(s) 830
FaeI CATG 4 cut(s) 23, 57, 77, 135
FaiI YATR 5 cut(s) 21, 55, 75, 98, 133
FaqI GGGAC 2 cut(s) 360, 468
FatI CATG 4 cut(s) 19, 53, 73, 131
FauI CCCGC 3 cut(s) 543, 582, 643
Fnu4HI GCNGC 6 cut(s) 174, 338, 348, 439, 474, 546
FokI GGATG 2 cut(s) 515, 554
Fsp4HI GCNGC 6 cut(s) 174, 338, 348, 439, 474, 546
FspBI CTAG 3 cut(s) 647, 908, 1090
GlaI GCGC 2 cut(s) 341, 351
GluI GCNGC 6 cut(s) 174, 338, 348, 439, 474, 546
GsuI CTGGAG 1 cut(s) 387
HaeIII GGCC 3 cut(s) 237, 283, 504
HapII CCGG 6 cut(s) 238, 387, 404, 481, 539, 562
HgaI GACGC 2 cut(s) 341, 547
HhaI GCGC 2 cut(s) 342, 352
Hin1II CATG 4 cut(s) 23, 57, 77, 135
Hin6I GCGC 2 cut(s) 340, 350
HinP1I GCGC 2 cut(s) 340, 350
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 1 cut(s) 373
HpaII CCGG 6 cut(s) 238, 387, 404, 481, 539, 562
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 4 cut(s) 29, 313, 322, 463
Hpy188III TCNNGA 3 cut(s) 404, 508, 562
Hpy8I GTNNAC 1 cut(s) 103
Hpy99I CGWCG 1 cut(s) 357
HpyAV CCTTC 2 cut(s) 8, 151
HpyCH4III ACNGT 2 cut(s) 459, 467
HpyCH4IV ACGT 1 cut(s) 839
HpyCH4V TGCA 4 cut(s) 23, 221, 254, 441
HpyF10VI GCNNNNNNNGC 3 cut(s) 323, 358, 674
HpyF3I CTNAG 9 cut(s) 153, 600, 678, 739, 800, 861, 922, 983, 1043
HpySE526I ACGT 1 cut(s) 839
Hsp92II CATG 4 cut(s) 23, 57, 77, 135
HspAI GCGC 2 cut(s) 340, 350
Kpn2I TCCGGA 2 cut(s) 403, 561
Kzo9I GATC 2 cut(s) 34, 106
LmnI GCTCC 2 cut(s) 435, 445
Lsp1109I GCAGC 2 cut(s) 185, 425
LweI GCATC 5 cut(s) 208, 241, 263, 313, 532
MaeI CTAG 3 cut(s) 647, 908, 1090
MaeII ACGT 1 cut(s) 839
MalI GATC 2 cut(s) 36, 108
MboI GATC 2 cut(s) 34, 106
MboII GAAGA 2 cut(s) 205, 404
MlsI TGGCCA 1 cut(s) 283
MluCI AATT 5 cut(s) 9, 30, 231, 242, 418
MluNI TGGCCA 1 cut(s) 283
MlyI GAGTC 1 cut(s) 382
MnlI CCTC 9 cut(s) 362, 411, 423, 444, 489, 515, 568, 760, 943
Mox20I TGGCCA 1 cut(s) 283
Mph1103I ATGCAT 1 cut(s) 256
MroI TCCGGA 2 cut(s) 403, 561
MscI TGGCCA 1 cut(s) 283
MseI TTAA 2 cut(s) 180, 230
MslI CAYNNNNRTG 1 cut(s) 332
Msp20I TGGCCA 1 cut(s) 283
MspI CCGG 6 cut(s) 238, 387, 404, 481, 539, 562
MspR9I CCNGG 2 cut(s) 366, 387
Mva1269I GAATGC 1 cut(s) 302
MvaI CCWGG 1 cut(s) 366
MvnI CGCG 3 cut(s) 342, 352, 476
MwoI GCNNNNNNNGC 3 cut(s) 323, 358, 674
NciI CCSGG 1 cut(s) 387
NdeII GATC 2 cut(s) 34, 106
NheI GCTAGC 2 cut(s) 646, 907
NlaIII CATG 4 cut(s) 23, 57, 77, 135
NmeAIII GCCGAG 1 cut(s) 18
NmuCI GTSAC 6 cut(s) 266, 614, 753, 814, 936, 997
NsiI ATGCAT 1 cut(s) 256
PctI GAATGC 1 cut(s) 302
PflFI GACNNNGTC 5 cut(s) 612, 751, 812, 934, 995
PkrI GCNGC 6 cut(s) 175, 339, 349, 440, 475, 547
PleI GAGTC 1 cut(s) 381
PpsI GAGTC 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 364
PspGI CCWGG 1 cut(s) 364
PspPI GGNCC 1 cut(s) 120
PsyI GACNNNGTC 5 cut(s) 612, 751, 812, 934, 995
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
RseI CAYNNNNRTG 1 cut(s) 332
SaqAI TTAA 2 cut(s) 180, 230
SatI GCNGC 6 cut(s) 174, 338, 348, 439, 474, 546
Sau3AI GATC 2 cut(s) 34, 106
Sau96I GGNCC 1 cut(s) 120
ScaI AGTACT 1 cut(s) 50
SchI GAGTC 1 cut(s) 382
ScrFI CCNGG 2 cut(s) 366, 387
SfaNI GCATC 5 cut(s) 208, 241, 263, 313, 532
SinI GGWCC 1 cut(s) 120
SmiMI CAYNNNNRTG 1 cut(s) 332
Sse9I AATT 5 cut(s) 9, 30, 231, 242, 418
SspMI CTAG 3 cut(s) 647, 908, 1090
StyD4I CCNGG 2 cut(s) 364, 385
StyI CCWWGG 2 cut(s) 769, 952
TaaI ACNGT 2 cut(s) 459, 467
TaiI ACGT 1 cut(s) 842
TaqI TCGA 1 cut(s) 216
TasI AATT 5 cut(s) 9, 30, 231, 242, 418
TatI WGTACW 1 cut(s) 48
TauI GCSGC 4 cut(s) 340, 350, 476, 548
Tru1I TTAA 2 cut(s) 180, 230
Tru9I TTAA 2 cut(s) 180, 230
TscAI CASTG 3 cut(s) 151, 253, 498
TseFI GTSAC 6 cut(s) 266, 614, 753, 814, 936, 997
TseI GCWGC 2 cut(s) 173, 438
Tsp45I GTSAC 6 cut(s) 266, 614, 753, 814, 936, 997
TspGWI ACGGA 4 cut(s) 458, 711, 772, 955
TspRI CASTG 3 cut(s) 151, 253, 498
Tth111I GACNNNGTC 5 cut(s) 612, 751, 812, 934, 995
VpaK11BI GGWCC 1 cut(s) 120
XapI RAATTY 1 cut(s) 242
XspI CTAG 3 cut(s) 647, 908, 1090
ZrmI AGTACT 1 cut(s) 50
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.