Rh2DG596500

Inner membrane component of T3SS, cytoplasmic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005560
Physical Location & Seq
Reverse (-)
82929418 .. 82936656
7239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG596500.1

Sequence Viewer

Length: 375 bp
ATGTCAACAGGAATGTCCTCAGATGAAGCATTGCATAAAGACATGCAGAAAAATTTCACATTGTTGAGCATACCAGCATCAACAAAAGAAGAAGTACACGAACCCAACACATTCCTCATAATTAAATCCACTATCCCGATTGCTCATATGAAGAAAGAGATACATGCTAGTCGTGCTAAAGACATCTCTCAAGGTGGATTGACTCAAGGGCAACAAACTCAGATCGCTCGAAATGAACAAAGAACAGAACAGATTCTGGAAGAGCTTGAAAATTTGGAAGAGACACTGAATGAGAGTATTCGAGAAAGTTTAGGTGCACGTGTAGGGAAGCTATCTCATGGTAAGAAGAAAGGAGCAATTGAAGAAGAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FHA

Protein Analysis

124

Amino Acids

13.82

Weight (kDa)

5.74

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 52, 271
AcvI CACGTG 1 cut(s) 320
AfaI GTAC 1 cut(s) 96
AflIII ACRYGT 1 cut(s) 319
AgsI TTSAA 2 cut(s) 269, 362
AluBI AGCT 2 cut(s) 265, 331
AluI AGCT 2 cut(s) 265, 331
Alw21I GWGCWC 1 cut(s) 319
Alw26I GTCTC 1 cut(s) 275
Alw44I GTGCAC 1 cut(s) 315
AlwNI CAGNNNCTG 1 cut(s) 256
ApaLI GTGCAC 1 cut(s) 315
ApoI RAATTY 2 cut(s) 52, 271
Asp700I GAANNNNTTC 2 cut(s) 53, 252
BaeGI GKGCMC 1 cut(s) 319
BbrPI CACGTG 1 cut(s) 320
Bbv12I GWGCWC 1 cut(s) 319
BcoDI GTCTC 1 cut(s) 275
BfaI CTAG 1 cut(s) 168
BmsI GCATC 1 cut(s) 86
BpuEI CTTGAG 2 cut(s) 174, 189
BsaAI YACGTR 1 cut(s) 320
Bse3DI GCAATG 1 cut(s) 29
BseMI GCAATG 1 cut(s) 29
BseMII CTCAG 2 cut(s) 33, 233
BseSI GKGCMC 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 319
BsmAI GTCTC 1 cut(s) 275
Bsp1286I GDGCHC 1 cut(s) 319
Bsp143I GATC 1 cut(s) 222
BspCNI CTCAG 2 cut(s) 32, 232
BspQI GCTCTTC 1 cut(s) 255
BsrDI GCAATG 1 cut(s) 29
BssMI GATC 1 cut(s) 222
Bst6I CTCTTC 2 cut(s) 255, 273
BstBAI YACGTR 1 cut(s) 320
BstDEI CTNAG 2 cut(s) 19, 219
BstKTI GATC 1 cut(s) 225
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 1 cut(s) 222
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstNSI RCATGY 2 cut(s) 46, 167
BstSLI GKGCMC 1 cut(s) 319
BtsIMutI CAGTG 1 cut(s) 284
CaiI CAGNNNCTG 1 cut(s) 256
Csp6I GTAC 1 cut(s) 95
CviAII CATG 3 cut(s) 43, 164, 338
CviJI RGCY 2 cut(s) 265, 331
CviKI_1 RGCY 2 cut(s) 265, 331
CviQI GTAC 1 cut(s) 95
DdeI CTNAG 2 cut(s) 19, 219
DpnI GATC 1 cut(s) 224
DpnII GATC 1 cut(s) 222
Eam1104I CTCTTC 2 cut(s) 255, 273
EarI CTCTTC 2 cut(s) 255, 273
Eco72I CACGTG 1 cut(s) 320
FaeI CATG 3 cut(s) 46, 167, 341
FaiI YATR 8 cut(s) 36, 44, 71, 119, 147, 149, 165, 339
FatI CATG 3 cut(s) 42, 163, 337
FauNDI CATATG 1 cut(s) 147
FspBI CTAG 1 cut(s) 168
Hin1II CATG 3 cut(s) 46, 167, 341
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 2 cut(s) 202, 253
Hpy166II GTNNAC 3 cut(s) 6, 97, 317
Hpy188I TCNGA 2 cut(s) 22, 222
Hpy188III TCNNGA 3 cut(s) 136, 257, 302
Hpy8I GTNNAC 3 cut(s) 6, 97, 317
HpyCH4IV ACGT 1 cut(s) 319
HpyCH4V TGCA 3 cut(s) 34, 46, 317
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpyF3I CTNAG 2 cut(s) 19, 219
HpySE526I ACGT 1 cut(s) 319
Hsp92II CATG 3 cut(s) 46, 167, 341
Kzo9I GATC 1 cut(s) 222
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 1 cut(s) 353
LpnPI CCDG 2 cut(s) 87, 242
LweI GCATC 1 cut(s) 86
MaeI CTAG 1 cut(s) 168
MaeII ACGT 1 cut(s) 319
MalI GATC 1 cut(s) 224
MboI GATC 1 cut(s) 222
MboII GAAGA 6 cut(s) 101, 163, 272, 290, 358, 374
MfeI CAATTG 1 cut(s) 357
MhlI GDGCHC 1 cut(s) 319
MluCI AATT 4 cut(s) 52, 120, 271, 357
MlyI GAGTC 1 cut(s) 196
MnlI CCTC 2 cut(s) 28, 125
MroXI GAANNNNTTC 2 cut(s) 53, 252
MseI TTAA 2 cut(s) 123, 373
MunI CAATTG 1 cut(s) 357
MwoI GCNNNNNNNGC 1 cut(s) 173
NdeI CATATG 1 cut(s) 147
NdeII GATC 1 cut(s) 222
NlaIII CATG 3 cut(s) 46, 167, 341
NspI RCATGY 2 cut(s) 46, 167
PciSI GCTCTTC 1 cut(s) 255
PdmI GAANNNNTTC 2 cut(s) 53, 252
PfeI GAWTC 1 cut(s) 253
PleI GAGTC 1 cut(s) 196
PmaCI CACGTG 1 cut(s) 320
PmlI CACGTG 1 cut(s) 320
PpsI GAGTC 1 cut(s) 196
Ppu21I YACGTR 1 cut(s) 320
PspCI CACGTG 1 cut(s) 320
PstNI CAGNNNCTG 1 cut(s) 256
RsaI GTAC 1 cut(s) 96
RsaNI GTAC 1 cut(s) 95
SapI GCTCTTC 1 cut(s) 255
SaqAI TTAA 2 cut(s) 123, 373
Sau3AI GATC 1 cut(s) 222
SchI GAGTC 1 cut(s) 196
SduI GDGCHC 1 cut(s) 319
SetI ASST 5 cut(s) 196, 267, 316, 322, 333
SfaNI GCATC 1 cut(s) 86
SmlI CTYRAG 2 cut(s) 189, 204
SmoI CTYRAG 2 cut(s) 189, 204
Sse9I AATT 4 cut(s) 52, 120, 271, 357
SspMI CTAG 1 cut(s) 168
TaiI ACGT 1 cut(s) 322
TaqI TCGA 2 cut(s) 229, 301
TasI AATT 4 cut(s) 52, 120, 271, 357
TatI WGTACW 1 cut(s) 94
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 2 cut(s) 123, 373
Tru9I TTAA 2 cut(s) 123, 373
TscAI CASTG 1 cut(s) 291
TspDTI ATGAA 3 cut(s) 39, 164, 249
TspRI CASTG 1 cut(s) 291
VneI GTGCAC 1 cut(s) 315
XapI RAATTY 2 cut(s) 52, 271
XceI RCATGY 2 cut(s) 46, 167
XmnI GAANNNNTTC 2 cut(s) 53, 252
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.