Rorug01G0234300

Methionine adenosyltransferase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001020
Physical Location & Seq
Reverse (-)
33681945 .. 33683287
1343 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0234300.1

Sequence Viewer

Length: 255 bp
ATGGTTCTAGGCGGTGGAGCAGCTCCCCCAAGAGGAAGTGCCGCAGCTACTGCAAGCATGCGCCGAAGGAGGACGACGAGTGGTGGCGGTGGTGCTTCTGGAGGTGCAGCCGGGACAATGCTCCAGTTCTACACTGACGATGCCCCAGGACTCAAGATCTCCCCGAATGTTGTTCTCATCATGAGCATTGGTTTCATTGCCTTTGTTGCTGTTCTTCATGTCATGGGAAAGCTCTACTTTGTGCGCTCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RmlD_sub_bind

Protein Analysis

84

Amino Acids

8.44

Weight (kDa)

11.38

Isoelectric Point (pI)

56.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec61_beta PF03911 37 - 76 1.8e-18 Sec61beta family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 12, 42, 87
AfiI CCNNNNNNNGG 1 cut(s) 32
AjnI CCWGG 1 cut(s) 145
AluBI AGCT 3 cut(s) 23, 47, 232
AluI AGCT 3 cut(s) 23, 47, 232
AlwNI CAGNNNCTG 1 cut(s) 50
ApeKI GCWGC 3 cut(s) 20, 44, 107
AspLEI GCGC 2 cut(s) 63, 246
AsuC2I CCSGG 1 cut(s) 112
BbvI GCAGC 3 cut(s) 32, 56, 119
BciT130I CCWGG 1 cut(s) 147
BcnI CCSGG 1 cut(s) 112
BfaI CTAG 1 cut(s) 8
BglII AGATCT 1 cut(s) 156
BisI GCNGC 4 cut(s) 21, 42, 45, 108
BlsI GCNGC 4 cut(s) 22, 43, 46, 109
Bme1390I CCNGG 2 cut(s) 112, 147
BmrFI CCNGG 2 cut(s) 112, 147
BmsI GCATC 1 cut(s) 130
BpmI CTGGAG 2 cut(s) 107, 120
BpuEI CTTGAG 1 cut(s) 137
BpuMI CCSGG 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 145
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 124
Bse3DI GCAATG 1 cut(s) 195
BseBI CCWGG 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 145
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMI GCAATG 1 cut(s) 195
BseNI ACTGG 1 cut(s) 124
BseXI GCAGC 3 cut(s) 32, 56, 119
BsgI GTGCAG 1 cut(s) 126
BsiSI CCGG 1 cut(s) 111
BslFI GGGAC 1 cut(s) 127
BslI CCNNNNNNNGG 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 127
Bsp143I GATC 1 cut(s) 156
BspACI CCGC 3 cut(s) 12, 42, 87
BspHI TCATGA 1 cut(s) 180
BsrDI GCAATG 1 cut(s) 195
BsrI ACTGG 1 cut(s) 124
BssECI CCNNGG 1 cut(s) 145
BssMI GATC 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 50
BstC8I GCNNGC 2 cut(s) 55, 59
BstDEI CTNAG 1 cut(s) 252
BstHHI GCGC 2 cut(s) 63, 246
BstKTI GATC 1 cut(s) 159
BstMBI GATC 1 cut(s) 156
BstMWI GCNNNNNNNGC 2 cut(s) 50, 206
BstNI CCWGG 1 cut(s) 147
BstNSI RCATGY 1 cut(s) 61
BstSCI CCNGG 2 cut(s) 110, 145
BstV1I GCAGC 3 cut(s) 32, 56, 119
BstX2I RGATCY 1 cut(s) 156
BstYI RGATCY 1 cut(s) 156
BtsIMutI CAGTG 1 cut(s) 132
Cac8I GCNNGC 2 cut(s) 55, 59
CaiI CAGNNNCTG 1 cut(s) 50
CciI TCATGA 1 cut(s) 180
CfoI GCGC 2 cut(s) 63, 246
CviAII CATG 4 cut(s) 58, 181, 218, 223
CviJI RGCY 4 cut(s) 23, 47, 110, 232
CviKI_1 RGCY 4 cut(s) 23, 47, 110, 232
DdeI CTNAG 1 cut(s) 252
DpnI GATC 1 cut(s) 158
DpnII GATC 1 cut(s) 156
EcoRII CCWGG 1 cut(s) 145
FaeI CATG 4 cut(s) 61, 184, 221, 226
FaiI YATR 4 cut(s) 59, 182, 219, 224
FalI AAGNNNNNCTT 2 cut(s) 221, 253
FaqI GGGAC 1 cut(s) 127
FatI CATG 4 cut(s) 57, 180, 217, 222
Fnu4HI GCNGC 4 cut(s) 21, 42, 45, 108
Fsp4HI GCNGC 4 cut(s) 21, 42, 45, 108
FspBI CTAG 1 cut(s) 8
GlaI GCGC 2 cut(s) 62, 245
GluI GCNGC 4 cut(s) 21, 42, 45, 108
GsuI CTGGAG 2 cut(s) 107, 120
HapII CCGG 1 cut(s) 111
HhaI GCGC 2 cut(s) 63, 246
Hin1II CATG 4 cut(s) 61, 184, 221, 226
Hin6I GCGC 2 cut(s) 61, 244
HinP1I GCGC 2 cut(s) 61, 244
HinfI GANTC 1 cut(s) 150
HpaII CCGG 1 cut(s) 111
Hpy188III TCNNGA 3 cut(s) 99, 154, 181
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 1 cut(s) 60
HpyCH4V TGCA 2 cut(s) 53, 107
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 206
HpyF3I CTNAG 1 cut(s) 252
Hsp92II CATG 4 cut(s) 61, 184, 221, 226
HspAI GCGC 2 cut(s) 61, 244
Kzo9I GATC 1 cut(s) 156
LmnI GCTCC 3 cut(s) 17, 28, 126
LpnPI CCDG 5 cut(s) 84, 124, 132, 137, 159
Lsp1109I GCAGC 3 cut(s) 32, 56, 119
LweI GCATC 1 cut(s) 130
MaeI CTAG 1 cut(s) 8
MalI GATC 1 cut(s) 158
MboI GATC 1 cut(s) 156
MboII GAAGA 1 cut(s) 206
MflI RGATCY 1 cut(s) 156
MlyI GAGTC 1 cut(s) 144
MnlI CCTC 3 cut(s) 26, 63, 95
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 2 cut(s) 112, 147
MvaI CCWGG 1 cut(s) 147
MwoI GCNNNNNNNGC 2 cut(s) 50, 206
NciI CCSGG 1 cut(s) 112
NdeII GATC 1 cut(s) 156
NlaIII CATG 4 cut(s) 61, 184, 221, 226
NspI RCATGY 1 cut(s) 61
PaeI GCATGC 1 cut(s) 61
PagI TCATGA 1 cut(s) 180
PkrI GCNGC 4 cut(s) 22, 43, 46, 109
PleI GAGTC 1 cut(s) 144
PpsI GAGTC 1 cut(s) 144
Psp6I CCWGG 1 cut(s) 145
PspGI CCWGG 1 cut(s) 145
PstNI CAGNNNCTG 1 cut(s) 50
PsuI RGATCY 1 cut(s) 156
SatI GCNGC 4 cut(s) 21, 42, 45, 108
Sau3AI GATC 1 cut(s) 156
SchI GAGTC 1 cut(s) 144
ScrFI CCNGG 2 cut(s) 112, 147
SetI ASST 4 cut(s) 25, 49, 106, 234
SfaNI GCATC 1 cut(s) 130
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
SphI GCATGC 1 cut(s) 61
SsiI CCGC 3 cut(s) 12, 42, 87
SspMI CTAG 1 cut(s) 8
StyD4I CCNGG 2 cut(s) 110, 145
TauI GCSGC 1 cut(s) 44
TscAI CASTG 1 cut(s) 139
TseI GCWGC 3 cut(s) 20, 44, 107
TspDTI ATGAA 2 cut(s) 184, 206
TspRI CASTG 1 cut(s) 139
XceI RCATGY 1 cut(s) 61
XspI CTAG 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.