Rw1G021460

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001020
Physical Location & Seq
Forward (+)
45714947 .. 45718894
3948 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G021460.1

Sequence Viewer

Length: 354 bp
ATGATGAAATCTGTCTTTAGTCCATTGCATTTGCAAGTCTCAATTGGAAAATCAAGTCAAGAAAGAGAGTTGGTGATCGGAGCAAATGGGAGGAGGCGAGCATGCGCACGGCGAAGGGGCCCACGGCGGAGACTTCAGGGCGAAGGTGTGGAGCATGAGCGGTGGGCCCTACTGCAGGCCCAAGCACTGGAAGCGCAACACGGCCTTCGCCATGTTCGGAGTCTTCCTCATCTGCATTCCCATCGCCATGAAATCTGCCGAGCTCGAGAGGCTAAGCAAAGAAAATTGCTACTGAATGTTGGTGGATCTGACAGGTATCCCGTGTTCAAATGGCTGAGACGGTTGCTGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

117

Amino Acids

13.65

Weight (kDa)

11.74

Isoelectric Point (pI)

77.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 106
AccB7I CCANNNNNTGG 1 cut(s) 187
AccBSI CCGCTC 1 cut(s) 160
AciI CCGC 2 cut(s) 127, 160
AclWI GGATC 1 cut(s) 313
AcuI CTGAAG 1 cut(s) 119
AfiI CCNNNNNNNGG 2 cut(s) 175, 187
AgsI TTSAA 1 cut(s) 328
AluBI AGCT 1 cut(s) 263
AluI AGCT 1 cut(s) 263
Alw21I GWGCWC 1 cut(s) 265
Alw26I GTCTC 3 cut(s) 43, 124, 331
AlwI GGATC 1 cut(s) 313
Ama87I CYCGRG 1 cut(s) 264
AoxI GGCC 4 cut(s) 118, 165, 177, 202
ApaI GGGCCC 2 cut(s) 122, 169
AspLEI GCGC 2 cut(s) 107, 196
AspS9I GGNCC 5 cut(s) 118, 119, 165, 166, 178
AsuHPI GGTGA 1 cut(s) 85
AvaI CYCGRG 1 cut(s) 264
BaeGI GKGCMC 2 cut(s) 122, 169
BanII GRGCYC 3 cut(s) 122, 169, 265
BbsI GAAGAC 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 265
BccI CCATC 1 cut(s) 249
BceAI ACGGC 3 cut(s) 125, 140, 217
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BciVI GTATCC 1 cut(s) 327
BcoDI GTCTC 3 cut(s) 43, 124, 331
BfmI CTRYAG 1 cut(s) 173
BfuI GTATCC 1 cut(s) 327
BlpI GCTNAGC 1 cut(s) 273
BmeT110I CYCGRG 1 cut(s) 264
BmgT120I GGNCC 5 cut(s) 118, 119, 165, 166, 178
BmiI GGNNCC 3 cut(s) 119, 120, 167
BpiI GAAGAC 1 cut(s) 215
BplI GAGNNNNNCTC 2 cut(s) 211, 243
Bpu1102I GCTNAGC 1 cut(s) 273
BsaJI CCNNGG 1 cut(s) 122
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 187
Bse1I ACTGG 1 cut(s) 192
Bse3DI GCAATG 1 cut(s) 23
BseDI CCNNGG 1 cut(s) 122
BseLI CCNNNNNNNGG 2 cut(s) 175, 187
BseMI GCAATG 1 cut(s) 23
BseMII CTCAG 1 cut(s) 326
BseNI ACTGG 1 cut(s) 192
BseRI GAGGAG 1 cut(s) 106
BseSI GKGCMC 2 cut(s) 122, 169
BshFI GGCC 4 cut(s) 120, 167, 179, 204
BsiHKAI GWGCWC 1 cut(s) 265
BsiHKCI CYCGRG 1 cut(s) 264
BslI CCNNNNNNNGG 2 cut(s) 175, 187
BsmAI GTCTC 3 cut(s) 43, 124, 331
BsmBI CGTCTC 1 cut(s) 331
BsmI GAATGC 1 cut(s) 235
BsnI GGCC 4 cut(s) 120, 167, 179, 204
BsoBI CYCGRG 1 cut(s) 264
Bsp120I GGGCCC 2 cut(s) 118, 165
Bsp1286I GDGCHC 3 cut(s) 122, 169, 265
Bsp143I GATC 2 cut(s) 75, 305
Bsp1720I GCTNAGC 1 cut(s) 273
BspACI CCGC 2 cut(s) 127, 160
BspANI GGCC 4 cut(s) 120, 167, 179, 204
BspCNI CTCAG 1 cut(s) 327
BspLI GGNNCC 3 cut(s) 119, 120, 167
BspMAI CTGCAG 1 cut(s) 177
BspPI GGATC 1 cut(s) 313
BsrBI CCGCTC 1 cut(s) 160
BsrDI GCAATG 1 cut(s) 23
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 1 cut(s) 122
BssMI GATC 2 cut(s) 75, 305
Bst4CI ACNGT 1 cut(s) 342
BstC8I GCNNGC 3 cut(s) 99, 103, 177
BstDEI CTNAG 2 cut(s) 273, 335
BstDSI CCRYGG 1 cut(s) 122
BstENI CCTNNNNNAGG 1 cut(s) 173
BstHHI GCGC 2 cut(s) 107, 196
BstKTI GATC 2 cut(s) 78, 308
BstMAI GTCTC 3 cut(s) 43, 124, 331
BstMBI GATC 2 cut(s) 75, 305
BstMWI GCNNNNNNNGC 2 cut(s) 191, 269
BstNSI RCATGY 1 cut(s) 105
BstSFI CTRYAG 1 cut(s) 173
BstSLI GKGCMC 2 cut(s) 122, 169
BstV2I GAAGAC 1 cut(s) 215
BstX2I RGATCY 1 cut(s) 305
BstYI RGATCY 1 cut(s) 305
BsuI GTATCC 1 cut(s) 327
BsuRI GGCC 4 cut(s) 120, 167, 179, 204
BtgI CCRYGG 1 cut(s) 122
BtgZI GCGATG 1 cut(s) 227
BtsIMutI CAGTG 1 cut(s) 185
Cac8I GCNNGC 3 cut(s) 99, 103, 177
CfoI GCGC 2 cut(s) 107, 196
Cfr13I GGNCC 5 cut(s) 118, 119, 165, 166, 178
CviAII CATG 4 cut(s) 102, 155, 212, 248
CviJI RGCY 7 cut(s) 120, 167, 179, 204, 263, 272, 334
CviKI_1 RGCY 7 cut(s) 120, 167, 179, 204, 263, 272, 334
DdeI CTNAG 2 cut(s) 273, 335
DpnI GATC 2 cut(s) 77, 307
DpnII GATC 2 cut(s) 75, 305
EciI GGCGGA 1 cut(s) 142
Ecl136II GAGCTC 1 cut(s) 263
Eco24I GRGCYC 3 cut(s) 122, 169, 265
Eco53kI GAGCTC 1 cut(s) 263
Eco57I CTGAAG 1 cut(s) 119
Eco88I CYCGRG 1 cut(s) 264
EcoICRI GAGCTC 1 cut(s) 263
EcoNI CCTNNNNNAGG 1 cut(s) 173
EcoO109I RGGNCCY 2 cut(s) 118, 166
EcoT38I GRGCYC 3 cut(s) 122, 169, 265
Esp3I CGTCTC 1 cut(s) 331
FaeI CATG 4 cut(s) 105, 158, 215, 251
FaiI YATR 5 cut(s) 103, 156, 213, 249, 352
FatI CATG 4 cut(s) 101, 154, 211, 247
FriOI GRGCYC 3 cut(s) 122, 169, 265
FspAI RTGCGCAY 1 cut(s) 106
FspI TGCGCA 1 cut(s) 106
GlaI GCGC 2 cut(s) 106, 195
HaeIII GGCC 4 cut(s) 120, 167, 179, 204
HhaI GCGC 2 cut(s) 107, 196
Hin1II CATG 4 cut(s) 105, 158, 215, 251
Hin6I GCGC 2 cut(s) 105, 194
HinP1I GCGC 2 cut(s) 105, 194
HinfI GANTC 1 cut(s) 220
HphI GGTGA 1 cut(s) 85
Hpy188I TCNGA 3 cut(s) 80, 219, 310
Hpy188III TCNNGA 2 cut(s) 59, 266
HpyAV CCTTC 3 cut(s) 108, 137, 215
HpyCH4III ACNGT 1 cut(s) 342
HpyCH4V TGCA 4 cut(s) 28, 34, 175, 235
HpyF10VI GCNNNNNNNGC 2 cut(s) 191, 269
HpyF3I CTNAG 2 cut(s) 273, 335
Hsp92II CATG 4 cut(s) 105, 158, 215, 251
HspAI GCGC 2 cut(s) 105, 194
Kzo9I GATC 2 cut(s) 75, 305
LmnI GCTCC 2 cut(s) 80, 151
LpnPI CCDG 4 cut(s) 122, 161, 173, 298
MalI GATC 2 cut(s) 77, 307
MbiI CCGCTC 1 cut(s) 160
MboI GATC 2 cut(s) 75, 305
MboII GAAGA 1 cut(s) 215
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 1 cut(s) 305
MhlI GDGCHC 3 cut(s) 122, 169, 265
MluCI AATT 2 cut(s) 42, 284
MlyI GAGTC 1 cut(s) 229
MnlI CCTC 4 cut(s) 84, 87, 237, 262
MslI CAYNNNNRTG 1 cut(s) 246
MunI CAATTG 1 cut(s) 42
Mva1269I GAATGC 1 cut(s) 235
MwoI GCNNNNNNNGC 2 cut(s) 191, 269
NdeII GATC 2 cut(s) 75, 305
NlaIII CATG 4 cut(s) 105, 158, 215, 251
NlaIV GGNNCC 3 cut(s) 119, 120, 167
NmeAIII GCCGAG 1 cut(s) 284
NsbI TGCGCA 1 cut(s) 106
NspI RCATGY 1 cut(s) 105
PaeI GCATGC 1 cut(s) 105
PaeR7I CTCGAG 1 cut(s) 264
PctI GAATGC 1 cut(s) 235
PflMI CCANNNNNTGG 1 cut(s) 187
PleI GAGTC 1 cut(s) 228
PpsI GAGTC 1 cut(s) 228
Psp124BI GAGCTC 1 cut(s) 265
PspN4I GGNNCC 3 cut(s) 119, 120, 167
PspOMI GGGCCC 2 cut(s) 118, 165
PspPI GGNCC 5 cut(s) 118, 119, 165, 166, 178
PsrI GAACNNNNNNTAC 2 cut(s) 308, 340
PstI CTGCAG 1 cut(s) 177
PsuI RGATCY 1 cut(s) 305
RseI CAYNNNNRTG 1 cut(s) 246
SacI GAGCTC 1 cut(s) 265
Sau3AI GATC 2 cut(s) 75, 305
Sau96I GGNCC 5 cut(s) 118, 119, 165, 166, 178
SchI GAGTC 1 cut(s) 229
SduI GDGCHC 3 cut(s) 122, 169, 265
SetI ASST 3 cut(s) 148, 265, 317
SfcI CTRYAG 1 cut(s) 173
Sfr274I CTCGAG 1 cut(s) 264
SlaI CTCGAG 1 cut(s) 264
SmiMI CAYNNNNRTG 1 cut(s) 246
SmlI CTYRAG 1 cut(s) 264
SmoI CTYRAG 1 cut(s) 264
SphI GCATGC 1 cut(s) 105
Sse9I AATT 2 cut(s) 42, 284
SsiI CCGC 2 cut(s) 127, 160
SstI GAGCTC 1 cut(s) 265
TaaI ACNGT 1 cut(s) 342
TaqI TCGA 1 cut(s) 265
TasI AATT 2 cut(s) 42, 284
TscAI CASTG 1 cut(s) 192
TspDTI ATGAA 2 cut(s) 20, 264
TspRI CASTG 1 cut(s) 192
Van91I CCANNNNNTGG 1 cut(s) 187
XagI CCTNNNNNAGG 1 cut(s) 173
XceI RCATGY 1 cut(s) 105
XhoI CTCGAG 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.