Rw1G021430

Methionine adenosyltransferase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001020
Physical Location & Seq
Forward (+)
45680868 .. 45683607
2740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G021430.1

Sequence Viewer

Length: 894 bp
ATGAGTAGGAAGCGAGTTTTGATACTTGGAGGAACAGGTTACTTGGGACAGCATGTATTGCAAGGCTTTTCAGAGATTCAAGAAACCACTCCTTGTGATCTGGCATTTACCCACCACTCAAGTCCTCCACCCCAAGCTTTGCTCGATGCATTTCCTAGTGTGATGCCTTTCTCTGTGGATTTGAAATCTGGCAATGGATTTGAAGCCATTTCTCATCGGTTTGGTCCGCCTGATGTGGTAGTAAACTGTGCTGCACTTTTGGTTCCTCATGCCTGTGAAATGGATCCTGCTGCAGCTATGTCAGTTAATGTGCCATCTTCTCTTGTTAATTGGTTATCGAGCCTAGAAGAGAGTAATTATCTTCTGATCCATTTGTCAACTGATCAAGTTTATGAAGGGGTGAAGTCCTTTTACAAGGAAGATGATGAAGTTGTTCCAGTAAATGTTTATGGGAAATCAAAAGTGGCAGCTGAGCAGTTCATTACTGAGAAATGCTCAAACTTTGCTATTTTGAGAAGTAGTATCATGTTTGGGCCACAGCCAATCTCACCGGTTTCAAAATCTCTTCCGATTCAGTGGGTTGATGGTGTCCTCTCCAAAGGAAATACAACGGAATTCTTTCATGATGAGTTTCGATGCCCTGTGTATGTAAAGGATGTCGTAGCAATCATACTTGCTTTGTCCAAGACATTGATATCAGAGGCTTTGCAAAGAAAATTGCTACTGAATGTTGGTGGACCGGACAGGGTATCCCGTGTACAAATGGCTGAGACGGTTGATCGTGGAGTTATGTCTCCTGCTGACATATCCATGGATATAACTAAGCTAGTTCAGACGCTTGGTATTTCTCGTATTTCATTTCGAGTTGGTGTCAGATTGACGCTTGAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RmlD_sub_bind

Protein Analysis

297

Amino Acids

32.48

Weight (kDa)

5.66

Isoelectric Point (pI)

46.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 5 - 259 8.7e-33 RmlD substrate binding domain
Epimerase PF01370 6 - 229 1.3e-17 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 7 - 163 5.5e-06 GDP-mannose 4,6 dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 227
AclWI GGATC 3 cut(s) 278, 291, 361
AcsI RAATTY 1 cut(s) 614
AfaI GTAC 1 cut(s) 759
AgeI ACCGGT 1 cut(s) 550
AgsI TTSAA 5 cut(s) 80, 184, 203, 558, 887
AluBI AGCT 4 cut(s) 137, 296, 470, 826
AluI AGCT 4 cut(s) 137, 296, 470, 826
Alw26I GTCTC 2 cut(s) 764, 798
AlwI GGATC 3 cut(s) 278, 291, 361
AoxI GGCC 1 cut(s) 533
ApeKI GCWGC 4 cut(s) 251, 290, 293, 467
ApoI RAATTY 1 cut(s) 614
AsiGI ACCGGT 1 cut(s) 550
Asp700I GAANNNNTTC 2 cut(s) 432, 618
AspS9I GGNCC 3 cut(s) 224, 533, 737
AsuHPI GGTGA 2 cut(s) 412, 540
AvaII GGWCC 2 cut(s) 224, 737
BamHI GGATCC 1 cut(s) 283
BarI GAAGNNNNNNTAC 2 cut(s) 395, 427
BbvI GCAGC 4 cut(s) 238, 277, 305, 479
BccI CCATC 2 cut(s) 322, 578
BciVI GTATCC 1 cut(s) 760
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 2 cut(s) 764, 798
BfaI CTAG 3 cut(s) 156, 344, 827
BfmI CTRYAG 1 cut(s) 291
BfuI GTATCC 1 cut(s) 760
BisI GCNGC 4 cut(s) 252, 291, 294, 468
BlpI GCTNAGC 1 cut(s) 471
BlsI GCNGC 4 cut(s) 253, 292, 295, 469
Bme18I GGWCC 2 cut(s) 224, 737
BmgT120I GGNCC 3 cut(s) 224, 533, 737
BmiI GGNNCC 2 cut(s) 264, 285
BmsI GCATC 3 cut(s) 136, 153, 626
BplI GAGNNNNNCTC 2 cut(s) 479, 511
Bpu1102I GCTNAGC 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 103
BsaJI CCNNGG 1 cut(s) 810
BsaWI WCCGGW 2 cut(s) 550, 739
Bse118I RCCGGY 1 cut(s) 550
Bse1I ACTGG 1 cut(s) 437
Bse3DI GCAATG 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 810
BseGI GGATG 1 cut(s) 661
BseMI GCAATG 1 cut(s) 199
BseMII CTCAG 3 cut(s) 462, 477, 759
BseNI ACTGG 1 cut(s) 437
BseXI GCAGC 4 cut(s) 238, 277, 305, 479
BsgI GTGCAG 1 cut(s) 237
BshFI GGCC 1 cut(s) 535
BshTI ACCGGT 1 cut(s) 550
BsiSI CCGG 2 cut(s) 551, 740
BslFI GGGAC 1 cut(s) 60
BsmAI GTCTC 2 cut(s) 764, 798
BsmBI CGTCTC 1 cut(s) 764
BsmFI GGGAC 1 cut(s) 60
BsnI GGCC 1 cut(s) 535
Bsp1407I TGTACA 1 cut(s) 757
Bsp143I GATC 5 cut(s) 97, 283, 366, 382, 778
Bsp1720I GCTNAGC 1 cut(s) 471
Bsp19I CCATGG 1 cut(s) 810
BspACI CCGC 1 cut(s) 227
BspANI GGCC 1 cut(s) 535
BspCNI CTCAG 3 cut(s) 463, 478, 760
BspHI TCATGA 1 cut(s) 622
BspLI GGNNCC 2 cut(s) 264, 285
BspMAI CTGCAG 1 cut(s) 295
BspPI GGATC 3 cut(s) 278, 291, 361
BsrDI GCAATG 1 cut(s) 199
BsrFI RCCGGY 1 cut(s) 550
BsrGI TGTACA 1 cut(s) 757
BsrI ACTGG 1 cut(s) 437
BssAI RCCGGY 1 cut(s) 550
BssECI CCNNGG 1 cut(s) 810
BssMI GATC 5 cut(s) 97, 283, 366, 382, 778
BssT1I CCWWGG 1 cut(s) 810
Bst4CI ACNGT 3 cut(s) 248, 775, 891
Bst6I CTCTTC 2 cut(s) 342, 570
BstAPI GCANNNNNTGC 1 cut(s) 58
BstAUI TGTACA 1 cut(s) 757
BstDEI CTNAG 4 cut(s) 471, 486, 768, 822
BstDSI CCRYGG 1 cut(s) 810
BstF5I GGATG 1 cut(s) 661
BstKTI GATC 5 cut(s) 100, 286, 369, 385, 781
BstMAI GTCTC 2 cut(s) 764, 798
BstMBI GATC 5 cut(s) 97, 283, 366, 382, 778
BstMWI GCNNNNNNNGC 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 56
BstSFI CTRYAG 1 cut(s) 291
BstV1I GCAGC 4 cut(s) 238, 277, 305, 479
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BsuI GTATCC 1 cut(s) 760
BsuRI GGCC 1 cut(s) 535
BtgI CCRYGG 1 cut(s) 810
BtsCI GGATG 1 cut(s) 661
BtsIMutI CAGTG 1 cut(s) 581
CciI TCATGA 1 cut(s) 622
Cfr10I RCCGGY 1 cut(s) 550
Cfr13I GGNCC 3 cut(s) 224, 533, 737
CseI GACGC 2 cut(s) 844, 889
Csp6I GTAC 1 cut(s) 758
CspAI ACCGGT 1 cut(s) 550
CviAII CATG 5 cut(s) 53, 269, 526, 623, 811
CviQI GTAC 1 cut(s) 758
DdeI CTNAG 4 cut(s) 471, 486, 768, 822
DpnI GATC 5 cut(s) 99, 285, 368, 384, 780
DpnII GATC 5 cut(s) 97, 283, 366, 382, 778
Eam1104I CTCTTC 2 cut(s) 342, 570
EarI CTCTTC 2 cut(s) 342, 570
EciI GGCGGA 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 810
Eco32I GATATC 1 cut(s) 696
Eco47I GGWCC 2 cut(s) 224, 737
EcoRI GAATTC 1 cut(s) 614
EcoRV GATATC 1 cut(s) 696
EcoT14I CCWWGG 1 cut(s) 810
EcoT22I ATGCAT 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 810
Esp3I CGTCTC 1 cut(s) 764
FaeI CATG 5 cut(s) 56, 272, 529, 626, 814
FaqI GGGAC 1 cut(s) 60
FatI CATG 5 cut(s) 52, 268, 525, 622, 810
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 4 cut(s) 252, 291, 294, 468
FokI GGATG 1 cut(s) 668
Fsp4HI GCNGC 4 cut(s) 252, 291, 294, 468
FspBI CTAG 3 cut(s) 156, 344, 827
GluI GCNGC 4 cut(s) 252, 291, 294, 468
HaeIII GGCC 1 cut(s) 535
HapII CCGG 2 cut(s) 551, 740
HgaI GACGC 2 cut(s) 844, 889
Hin1II CATG 5 cut(s) 56, 272, 529, 626, 814
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HindIII AAGCTT 1 cut(s) 135
HinfI GANTC 2 cut(s) 76, 571
HpaII CCGG 2 cut(s) 551, 740
HphI GGTGA 2 cut(s) 412, 540
Hpy166II GTNNAC 4 cut(s) 244, 378, 737, 758
Hpy188I TCNGA 6 cut(s) 73, 366, 570, 700, 834, 875
Hpy188III TCNNGA 2 cut(s) 80, 623
Hpy8I GTNNAC 4 cut(s) 244, 378, 737, 758
HpyAV CCTTC 1 cut(s) 389
HpyCH4III ACNGT 3 cut(s) 248, 775, 891
HpyCH4V TGCA 5 cut(s) 61, 149, 254, 293, 709
HpyF10VI GCNNNNNNNGC 1 cut(s) 58
HpyF3I CTNAG 4 cut(s) 471, 486, 768, 822
Hsp92II CATG 5 cut(s) 56, 272, 529, 626, 814
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 5 cut(s) 97, 283, 366, 382, 778
Lsp1109I GCAGC 4 cut(s) 238, 277, 305, 479
LweI GCATC 3 cut(s) 136, 153, 626
MaeI CTAG 3 cut(s) 156, 344, 827
MaeIII GTNAC 1 cut(s) 38
MalI GATC 5 cut(s) 99, 285, 368, 384, 780
MboI GATC 5 cut(s) 97, 283, 366, 382, 778
MboII GAAGA 5 cut(s) 309, 353, 359, 431, 557
MflI RGATCY 1 cut(s) 283
MluCI AATT 4 cut(s) 328, 355, 614, 716
MnlI CCTC 5 cut(s) 23, 135, 276, 602, 694
Mph1103I ATGCAT 1 cut(s) 151
MroXI GAANNNNTTC 2 cut(s) 432, 618
MseI TTAA 2 cut(s) 306, 327
MslI CAYNNNNRTG 2 cut(s) 273, 809
MspA1I CMGCKG 1 cut(s) 470
MspI CCGG 2 cut(s) 551, 740
MwoI GCNNNNNNNGC 1 cut(s) 58
NcoI CCATGG 1 cut(s) 810
NdeII GATC 5 cut(s) 97, 283, 366, 382, 778
NlaIII CATG 5 cut(s) 56, 272, 529, 626, 814
NlaIV GGNNCC 2 cut(s) 264, 285
NsiI ATGCAT 1 cut(s) 151
NspI RCATGY 1 cut(s) 56
PagI TCATGA 1 cut(s) 622
PdmI GAANNNNTTC 2 cut(s) 432, 618
PfeI GAWTC 2 cut(s) 76, 571
PinAI ACCGGT 1 cut(s) 550
PkrI GCNGC 4 cut(s) 253, 292, 295, 469
PspN4I GGNNCC 2 cut(s) 264, 285
PspPI GGNCC 3 cut(s) 224, 533, 737
PstI CTGCAG 1 cut(s) 295
PsuI RGATCY 1 cut(s) 283
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 1 cut(s) 759
RsaNI GTAC 1 cut(s) 758
RseI CAYNNNNRTG 2 cut(s) 273, 809
SaqAI TTAA 2 cut(s) 306, 327
SatI GCNGC 4 cut(s) 252, 291, 294, 468
Sau3AI GATC 5 cut(s) 97, 283, 366, 382, 778
Sau96I GGNCC 3 cut(s) 224, 533, 737
SetI ASST 5 cut(s) 40, 139, 298, 472, 828
SfaNI GCATC 3 cut(s) 136, 153, 626
SfcI CTRYAG 1 cut(s) 291
SinI GGWCC 2 cut(s) 224, 737
SmiMI CAYNNNNRTG 2 cut(s) 273, 809
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
Sse9I AATT 4 cut(s) 328, 355, 614, 716
SsiI CCGC 1 cut(s) 227
SspMI CTAG 3 cut(s) 156, 344, 827
StyI CCWWGG 1 cut(s) 810
TaaI ACNGT 3 cut(s) 248, 775, 891
TaqI TCGA 4 cut(s) 144, 338, 634, 862
TasI AATT 4 cut(s) 328, 355, 614, 716
TatI WGTACW 1 cut(s) 757
TfiI GAWTC 2 cut(s) 76, 571
Tru1I TTAA 2 cut(s) 306, 327
Tru9I TTAA 2 cut(s) 306, 327
TscAI CASTG 1 cut(s) 581
TseI GCWGC 4 cut(s) 251, 290, 293, 467
TspDTI ATGAA 5 cut(s) 408, 441, 469, 611, 846
TspGWI ACGGA 1 cut(s) 626
TspRI CASTG 1 cut(s) 581
VpaK11BI GGWCC 2 cut(s) 224, 737
XapI RAATTY 1 cut(s) 614
XceI RCATGY 1 cut(s) 56
XmnI GAANNNNTTC 2 cut(s) 432, 618
XspI CTAG 3 cut(s) 156, 344, 827
Zsp2I ATGCAT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.